100 Water 1 100 Water 1 8 Additive 2 0.2 4.1 Buffer 3 4 0.2 4.6 Buffer 3 4 0.2 5.1 Buffer 3 4 0.2 4.2 Buffer 5 6 0.2 4.7 Buffer 5 6 0.2 5.2 Buffer 5 6 0.2 4.4 Buffer 7 8 0.2 4.9 Buffer 7 8 0.2 5.4 Buffer 7 8 0.2 4.3 Buffer 10 11 0.2 4.8 Buffer 10 11 0.2 5.3 Buffer 10 11 0.2 4.3 Buffer 12 13 0.2 4.8 Buffer 12 13 0.2 5.3 Buffer 12 13 0.2 4.3 Buffer 14 15 0.2 4.8 Buffer 14 15 0.2 5.3 Buffer 14 15 0.2 5.2 Buffer 16 17 0.2 5.7 Buffer 16 17 0.2 6.2 Buffer 16 17 0.2 5.5 Buffer 3 4 0.2 6 Buffer 3 4 0.2 6.5 Buffer 3 4 0.2 5.6 Buffer 7 8 0.2 6.1 Buffer 7 8 0.2 6.6 Buffer 7 9 0.2 5.6 Buffer 18 19 0.2 6.1 Buffer 18 19 0.2 6.6 Buffer 18 19 0.2 5.7 Buffer 20 21 0.2 6.2 Buffer 20 21 0.2 6.7 Buffer 20 21 0.2 5.7 Buffer 22 23 0.2 6.2 Buffer 22 23 0.2 6.7 Buffer 22 23 0.2 6.1 Buffer 24 25 0.2 6.6 Buffer 24 25 0.2 7.1 Buffer 24 25 0.2 6.1 Buffer 26 27 0.2 6.6 Buffer 26 27 0.2 7.1 Buffer 26 27 0.2 6.3 Buffer 28 29 0.2 6.8 Buffer 28 29 0.2 7.3 Buffer 28 29 0.2 6.3 Buffer 30 31 0.2 6.8 Buffer 30 31 0.2 7.3 Buffer 30 31 0.2 6.3 Buffer 32 33 0.2 6.8 Buffer 32 33 0.2 7.3 Buffer 32 33 0.2 6.6 Buffer 34 35 0.2 7.1 Buffer 34 35 0.2 7.6 Buffer 34 35 0.2 6.6 Buffer 36 37 0.2 7.1 Buffer 36 37 0.2 7.6 Buffer 36 37 0.2 6.6 Buffer 38 39 0.2 7.1 Buffer 38 39 0.2 7.6 Buffer 38 39 0.2 7 Buffer 40 41 0.2 7.5 Buffer 40 41 0.2 8 Buffer 40 41 0.2 7.5 Buffer 42 43 0.2 8 Buffer 42 43 0.2 8.5 Buffer 42 43 0.2 7.5 Buffer 44 45 0.2 8 Buffer 44 45 0.2 8.5 Buffer 44 45 0.2 7.7 Buffer 46 47 0.2 8.2 Buffer 46 47 0.2 8.7 Buffer 46 47 0.2 7.7 Buffer 48 49 0.2 8.2 Buffer 48 49 0.2 8.7 Buffer 48 49 0.2 7.9 Buffer 50 51 0.2 8.4 Buffer 50 51 0.2 8.9 Buffer 50 51 0.2 8.5 Buffer 38 39 0.2 9 Buffer 38 39 0.2 9.5 Buffer 38 39 0.2 8.6 Buffer 52 53 0.2 9.1 Buffer 52 53 0.2 9.6 Buffer 52 53 0.2 8.8 Buffer 54 55 0.2 9.3 Buffer 54 55 0.2 9.8 Buffer 54 55 0.2 9.2 Buffer 56 57 0.2 9.7 Buffer 56 57 0.2 10.2 Buffer 56 57 0.2 9.9 Buffer 58 59 0.2 10.4 Buffer 58 59 0.2 10.9 Buffer 58 59 1 100 0 100 v/v false Water Water Water 2 8 0 8 M false 51457 Sigma Additive 57-13-6 Urea Urea 3 1 0 1 M 4 true Jena Bioscience Citric acid, pH has been adjusted to 4.0 using NaOH 4 1 0 1 M 6.5 true CB-Buffer-18 Axygen trisodium citrate buffer BCC ~citric acid sodium citrate ~citric acid-sodium citrate ~Na3 citrate/citric acid ~Sodium Citrate / Citric Acid ~tri-sodium citrate - citric acid ~trisodium Citrate-Citric Acid Buffer 8.1 0 6.68 0.02 6.39 0.04 6.2 0.06 6.06 0.08 5.98 0.1 5.9 0.12 5.82 0.14 5.71 0.16 5.62 0.18 5.55 0.2 5.45 0.22 5.36 0.24 5.28 0.26 5.21 0.28 5.09 0.3 5.02 0.32 4.96 0.34 4.88 0.36 4.81 0.38 4.72 0.4 4.62 0.42 4.55 0.44 4.45 0.46 4.39 0.48 4.32 0.5 4.22 0.52 4.14 0.54 4.08 0.56 4 0.58 3.9 0.6 3.81 0.62 3.74 0.64 3.64 0.66 3.57 0.68 3.51 0.7 3.39 0.72 3.32 0.74 3.27 0.76 3.18 0.78 3.11 0.8 3.04 0.82 2.96 0.84 2.9 0.86 2.83 0.88 2.72 0.9 2.64 0.92 2.55 0.94 2.46 0.96 2.42 0.98 2.3 1 68-04-2 77-92-9 tri-Sodium citrate Na3 Cit 5 1 0 1 M 4 true Molecular Dimensions Titrated to pH 4.0 using HCl 6 1 0 1 M 5.5 true Molecular Dimensions Titrated to pH 5.5 using HCl sodium acetate buffer BCC ~acetic acid-sodium acetate ~NaAc/acetic acid ~Sodium Acetate / Acetic Acid ~Sodium Acetate-Acetic Acid Buffer 4.8 127-09-3 Sodium acetate Na Acet 7 1 0 1 M 4.3 true Hampton Research 8 1 0 1 M 6.5 true CSS-307 Jena Bioscience 9 1 0 1 M 7 true 133279 Qiagen butanedioic acid BCC Buffer 5.6 110-15-6 di-Sodium succinate Na2 Succ 10 3 0 3 M 2.9 true Microlytic 11 3 0 3 M 7 true Jena Bioscience DL Malic acid, titrated with NaOH Buffer 10.16 0.332477 6.98 0.33303 6.6 0.333585 6.4 0.334142 6.25 0.3347 6.16 0.335261 6.08 0.335823 6.03 0.336387 5.95 0.336953 5.88 0.337521 5.82 0.338091 5.79 0.338663 5.75 0.339237 5.67 0.34039 5.6 0.341552 5.55 0.342721 5.5 0.343898 5.45 0.345084 5.4 0.346277 5.36 0.347479 5.29 0.349908 5.22 0.352371 5.17 0.354869 5.12 0.357403 5.05 0.360622 4.99 0.363899 4.94 0.367236 4.89 0.370635 4.84 0.374097 4.79 0.377625 4.75 0.38122 4.71 0.384884 4.67 0.388619 4.62 0.392427 4.58 0.396311 4.55 0.400272 4.51 0.404313 4.48 0.408437 4.44 0.412646 4.4 0.416942 4.36 0.421329 4.32 0.425809 4.29 0.430385 4.26 0.435061 4.22 0.43984 4.18 0.444724 4.15 0.449719 4.11 0.454826 4.05 0.463245 3.99 0.47198 3.93 0.481052 3.87 0.490479 3.82 0.500283 3.76 0.510487 3.71 0.521116 3.65 0.532197 3.59 0.543759 3.53 0.555835 3.5 0.563659 3.46 0.571706 3.42 0.579986 3.39 0.58851 3.35 0.597287 3.31 0.606331 3.27 0.615653 3.24 0.625265 3.2 0.635183 3.17 0.645421 3.13 0.655993 3.09 0.666918 3.05 0.678213 3.01 0.689898 2.97 0.701991 2.92 0.714517 2.88 0.727497 2.84 0.740958 2.79 0.754927 2.74 0.769432 2.7 0.781444 2.66 0.793836 2.61 0.806628 2.56 0.81984 2.51 0.833491 2.46 0.847604 2.4 0.862204 2.37 0.869694 2.34 0.877315 2.31 0.885071 2.27 0.892965 2.23 0.901002 2.19 0.909184 6915-15-7 di-Sodium DL-malate Na2 Mal (DL) 12 1 0 1 M 4 true 13 1 0 1 M 5.6 true 133195 Qiagen Buffer 4.2 868-18-8 di-Sodium tartrate Na2 Tart 14 1 0 1 M 4 true 15 1 0 1 M 6 true Propionic Acid Buffer 4.87 Sodium Propionate PropAcid 16 3.4 0 3.4 M 5 true 133243 Qiagen 17 3.4 0 3.4 M 7 true 133245 Qiagen disodium malonate buffer BCC disodium malonate salt BCC ~malonic acid-sodium malonate ~sodium malonate-malonic acid Buffer 5.5 di-Sodium malonate Na2 Malon 18 1 0 1 M 5 true Molecular Dimensions 19 1 0 1 M 7 true 133210 Qiagen MES acid, titrated with NaOH MES BCC ~MES sodium salt ~MES-NaOH ~MES/NaOH ~Na MES ~NaMES ~sodium MES Buffer 6.1 71119-23-8 MES MES 20 1 0 1 M 4.5 true 21 1 0 1 M 7 true Maleic Acid buffer Sodium maleate Buffer 6.26 371-47-1 di-Sodium maleate Na2 Male 22 1 0 1 M 5.5 true Molecular Dimensions 23 1 0 1 M 6.7 true Molecular Dimensions ~cacodylate ~na cacodylate ~Na Cacodylate/HCl ~Na-cacodylate ~sodium dimethyl arsonide Buffer 6.2 124-65-2 Sodium cacodylate Na Cacod 24 1 0 1 M 6 true Molecular Dimensions 25 1 0 1 M 7.5 true HR2-255-20 Hampton Research Buffer 6.6 26239-55-4 ADA ADA 26 1 0 1 M 5.5 true Molecular Dimensions 27 1 0 1 M 8 true Molecular Dimensions bis-tris buffer BCC ~bis tris chloride ~bis-tris chloride Buffer 6.5 6976-37-0 BIS-TRIS BIS-TRIS 28 1 0 1 M 6.1 true Sigma 29 1 0 1 M 7.5 true Sigma Buffer 6.8 7365-82-4 N-(2-Acetamido)-2-aminoethanesulfonic acid ACES 30 3 0 3 M 6.2 true 133253 Qiagen 31 3 0 3 M 7.5 true Buffer 7.2 7558-80-7 Sodium di-hydrogen phosphate NaH2PO4 32 1 0 1 M 6 true Molecular Dimensions 33 1 0 1 M 7.5 true Hampton Research Piperazine-1,4-bis(2-ethanesulfonic acid) Buffer 6.8 100037-69-2 PIPES PIPES 34 1 0 1 M 6.2 true Molecular Dimensions pH has been adjusted to 6.2 using HCl 35 1 0 1 M 8 true Molecular Dimensions Buffer 7 288-32-4 Imidazole Imidazole 36 1 0 1 M 6.5 true Molecular Dimensions 37 1 0 1 M 7.9 true Molecular Dimensions MOPS BCC Buffer 7.2 71119-22-7 MOPS MOPS 38 1 0 1 M 6.5 true Molecular Dimensions 39 1 0 1 M 9.7 true HR2-833 Hampton Research 1,3-Bis[tris(hydroxymethyl)methylamino]propane Buffer 6.8 64431-96-5 BIS-TRIS propane BIS-TRIS prop 40 1 0 1 M 6.5 true Molecular Dimensions 41 1 0 1 M 8.5 true Molecular Dimensions HEPES BCC ~HEPES - Na ~HEPES - Sodium ~HEPES sodium ~HEPES sodium salt ~HEPES-na ~HEPES-NaOH ~HEPES/NaOH ~Na HEPES ~na-HEPES Buffer 7.5 75277-39-3 HEPES HEPES 42 1 0 1 M 7.4 true Molecular Dimensions 43 1 0 1 M 8.8 true Molecular Dimensions Buffer 8.1 5704-04-1 TRICINE TRICINE 44 1 0 1 M 7.3 true Sigma 45 1 0 1 M 8.7 true Sigma 3-[4-(2-Hydroxyethyl)-1-piperazine]propanesulfonic 4-(2-Hydroxyethyl)-1-Piperazinepropanesulfonic aci Buffer 8 16052-06-5 EPPS EPPS 46 1 0 1 M 7.5 true Molecular Dimensions 47 1 0 1 M 9 true Molecular Dimensions TRIS Tris chloride tris chlorides BCC Tris-chloride ~Tris -HCl ~Tris base/HCl ~Tris HCl ~Tris- HCl ~tris-hcl ~tris-hydrochloride ~tris.hcl ~trishcl ~Trizma base ~~tris hydrochloride Buffer 8.1 1185-53-1 tris chloride BCC BCCTris-HCl 48 1 0 1 M 7.4 true Molecular Dimensions 49 1 0 1 M 9.3 true Molecular Dimensions Buffer 8.3 150-25-4 BICINE BICINE 50 1 0 1 M 7.7 true Sigma 51 1 0 1 M 9 true Fluidigm N-[Tris(hydroxymethyl)methyl]-3-amino propanesul Buffer 8.4 29915-38-6 TAPS TAPS 52 1 0 1 M 8.5 true Molecular Dimensions 53 1 0 1 M 11 true C3sodium tetraborate na borate Sodium borate Sodium tetraborate decahydrate Buffer 9.1 12007-42-0 1330-43-4 di-Sodium tetraborate Na2B4O7 54 1 0 1 M 8 true Emerald 55 1 0 1 M 10 true Molecular Dimensions ~CHES/NaOH ~sodium CHES ~~2-(Cyclohexylamino)ethanesulfonic acid Buffer 9.5 103-47-9 CHES CHES 56 1 0 1 M 9 true Molecular Dimensions 57 1 0 1 M 10.5 true Qiagen Buffer 9.8 56-40-6 Glycine Gly 58 1 0 1 M 9.5 true Molecular Dimensions 59 1 0 1 M 11.5 true Molecular Dimensions Buffer 10.5 1135-40-6 CAPS CAPS