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Supplemental Information

Screen Description

Membrane 1

Membrane / Membrane 1
Created 2009-11-16.

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Membrane 1

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Membrane 1

    Screen Structure

    Membrane 1

    Well Count 24
    Number of distinct ingredients 28
    Internal diversity (score mean dist) 0.916

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:09:36.453114 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Membrane 1
    Buffer 19
    Cryoprotectant 4
    Organic (non-volatile) 11
    Organic (volatile) 1
    Polymer 25
    Salt 26

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Membrane 1 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    • Glycerol 15 w/v -
    • Polyethylene glycol 400 15 w/v -
    		pH 7.5
    300 μl of Glycerol (50 w/v)
    200 μl of Calcium chloride (1 M)
    300 μl of Polyethylene glycol 400 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium chloride 0.02 M -
    • Polyethylene glycol 400 20 w/v -
    • Sodium chloride 0.1 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    8 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium chloride (1 M)
    400 μl of Polyethylene glycol 400 (50 w/v)
    392 μl of water (100 v/v)
    A3
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 4.6
    • Polyethylene glycol 400 25 w/v -
    		pH 4.6
    25 μl of Magnesium acetate (2 M)
    50 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Polyethylene glycol 400 (50 w/v)
    425 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.05 M pH 8.5
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol 400 30 w/v -
    • Sodium sulfate 0.05 M -
    		pH 8.5
    50 μl of Sodium sulfate (1 M)
    600 μl of Polyethylene glycol 400 (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    16 μl of Lithium sulfate (3 M)
    283 μl of water (100 v/v)
    A5
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 48 w/v -
    		pH 7.5
    640 μl of Polyethylene glycol 400 (75 w/v)
    200 μl of Calcium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    60 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.01 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 20 w/v -
    		pH 7.5
    400 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    3 μl of tris chloride BCC pH 7.5 (3 M)
    596 μl of water (100 v/v)
    B1
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol monomethyl ether 550 30 w/v -
    		pH 8.5
    600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    310 μl of water (100 v/v)
    B2
    • Polyethylene glycol 600 35 w/v -
    		NA
    700 μl of Polyethylene glycol 600 (50 w/v)
    300 μl of water (100 v/v)
    B3
    • TRICINE 0.1 M pH 8
    • Sodium chloride 0.35 M -
    • Polyethylene glycol 1,000 28 w/v -
    • Glycerol 10 w/v -
    		pH 8
    200 μl of Glycerol (50 w/v)
    100 μl of TRICINE pH 8 (1 M)
    70 μl of Sodium chloride (5 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    70 μl of water (100 v/v)
    B4
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 1,500 10 w/v -
    • Ethanol 5 w/v -
    		NA
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium chloride (1 M)
    75 μl of Ethanol (66 w/v)
    584 μl of water (100 v/v)
    B5
    • Polyethylene glycol 1,500 30 w/v -
    		NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • Polyethylene glycol 2,000 5 w/v -
    		NA
    100 μl of Polyethylene glycol 2,000 (50 w/v)
    900 μl of water (100 v/v)
    C1
    • Magnesium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 2,000 10 w/v -
    		pH 8.5
    200 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C2
    • Polyethylene glycol 2,000 15 w/v -
    		NA
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    700 μl of water (100 v/v)
    C3
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 2,000 15 w/v -
    		NA
    20 μl of Lithium chloride (5 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    680 μl of water (100 v/v)
    C4
    • tri-Sodium citrate 0.02 M -
    • di-Sodium hydrogen phosphate 0.1 M pH 6.2
    • Polyethylene glycol 2,000 15 w/v -
    		pH 6.2
    10 μl of tri-Sodium citrate (2 M)
    100 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    590 μl of water (100 v/v)
    C5
    • Sodium chloride 0.5 M -
    • di-Sodium hydrogen phosphate 0.1 M pH 6.8
    • Polyethylene glycol 2,000 15 w/v -
    		pH 6.8
    100 μl of Sodium chloride (5 M)
    100 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C6
    • BIS-TRIS 0.02 M pH 7
    • Polyethylene glycol 2,000 15 w/v -
    		pH 7
    20 μl of BIS-TRIS pH 7 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    680 μl of water (100 v/v)
    D1
    • Magnesium chloride 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 2,000 15 w/v -
    		pH 7.5
    40 μl of Magnesium chloride (2.5 M)
    50 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    610 μl of water (100 v/v)
    D2
    • tris chloride BCC 0.1 M pH 8
    • Magnesium nitrate 0.3 M -
    • Polyethylene glycol 2,000 20 w/v -
    • 2-methyl-2,4-pentanediol BCC 2 w/v -
    		pH 8
    300 μl of Magnesium nitrate (1 M)
    22 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    177 μl of water (100 v/v)
    D3
    • BICINE 0.1 M pH 9
    • Magnesium chloride 0.3 M -
    • Polyethylene glycol 2,000 25 w/v -
    • Glycerol 15 w/v -
    		pH 9
    120 μl of Magnesium chloride (2.5 M)
    187 μl of Glycerol (80 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of Polyethylene glycol 2,000 (50 w/v)
    92 μl of water (100 v/v)
    D4
    • Sodium chloride 0.5 M -
    • di-Sodium hydrogen phosphate 0.2 M pH 6.2
    • Polyethylene glycol 2,000 30 w/v -
    		pH 6.2
    200 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M)
    100 μl of Sodium chloride (5 M)
    600 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of water (100 v/v)
    D5
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 8 w/v -
    		pH 4.6
    160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    D6
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 200 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Glycerol 20 w/v -
    		pH 5.6
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    60 μl of Polyethylene glycol 200 (50 w/v)
    250 μl of Glycerol (80 w/v)
    390 μl of water (100 v/v)