C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Membrane 1

Membrane / Membrane 1

Created 2009-11-16.

Export

Membrane 1

Barcodes

Membrane 1

No barcodes found.

Screen Structure

Membrane 1

Well Count	24
Number of distinct ingredients	28
Internal diversity (score mean dist)	0.916

Screen Composition

More information about screen composition chart.

ingredient type	Membrane 1
Buffer	19
Cryoprotectant	4
Organic (non-volatile)	11
Organic (volatile)	1
Polymer	25
Salt	26

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Membrane 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - Glycerol 15 w/v - Polyethylene glycol 400 15 w/v - 🔍	pH 7.5	200.0 μl of Calcium chloride (1 M) 300.0 μl of Glycerol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of water (100 v/v)
A2	tri-Sodium citrate 0.1 M pH 5.6 Magnesium chloride 0.02 M - Polyethylene glycol 400 20 w/v - Sodium chloride 0.1 M - 🔍	pH 5.6	8.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 400 (50 w/v) 100.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 392.0 μl of water (100 v/v)
A3	Magnesium acetate 0.05 M - Sodium acetate 0.05 M pH 4.6 Polyethylene glycol 400 25 w/v - 🔍	pH 4.6	25.0 μl of Magnesium acetate (2 M) 500.0 μl of Polyethylene glycol 400 (50 w/v) 50.0 μl of Sodium acetate pH 4.6 (1 M) 425.0 μl of water (100 v/v)
A4	tris chloride BCC 0.05 M pH 8.5 Lithium sulfate 0.05 M - Polyethylene glycol 400 30 w/v - Sodium sulfate 0.05 M - 🔍	pH 8.5	16.7 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 50.0 μl of Sodium sulfate (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 283.3 μl of water (100 v/v)
A5	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 48 w/v - 🔍	pH 7.5	200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 640.0 μl of Polyethylene glycol 400 (75 w/v) 60.0 μl of water (100 v/v)
A6	tris chloride BCC 0.01 M pH 7.5 Polyethylene glycol monomethyl ether 550 20 w/v - 🔍	pH 7.5	400.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 3.3 μl of tris chloride BCC pH 7.5 (3 M) 596.7 μl of water (100 v/v)
B1	Magnesium chloride 0.1 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol monomethyl ether 550 30 w/v - 🔍	pH 8.5	40.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 310.0 μl of water (100 v/v)
B2	Polyethylene glycol 600 35 w/v - 🔍	NA	700.0 μl of Polyethylene glycol 600 (50 w/v) 300.0 μl of water (100 v/v)
B3	TRICINE 0.1 M pH 8 Sodium chloride 0.35 M - Polyethylene glycol 1,000 28 w/v - Glycerol 10 w/v - 🔍	pH 8	200.0 μl of Glycerol (50 w/v) 560.0 μl of Polyethylene glycol 1,000 (50 w/v) 70.0 μl of Sodium chloride (5 M) 100.0 μl of TRICINE pH 8 (1 M) 70.0 μl of water (100 v/v)
B4	Magnesium chloride 0.1 M - Sodium chloride 0.1 M - Polyethylene glycol 1,500 10 w/v - Ethanol 5 w/v - 🔍	NA	75.8 μl of Ethanol (66 w/v) 40.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of Sodium chloride (1 M) 584.2 μl of water (100 v/v)
B5	Polyethylene glycol 1,500 30 w/v - 🔍	NA	600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
B6	Polyethylene glycol 2,000 5 w/v - 🔍	NA	100.0 μl of Polyethylene glycol 2,000 (50 w/v) 900.0 μl of water (100 v/v)
C1	Magnesium chloride 0.5 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 2,000 10 w/v - 🔍	pH 8.5	200.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
C2	Polyethylene glycol 2,000 15 w/v - 🔍	NA	300.0 μl of Polyethylene glycol 2,000 (50 w/v) 700.0 μl of water (100 v/v)
C3	Lithium chloride 0.1 M - Polyethylene glycol 2,000 15 w/v - 🔍	NA	20.0 μl of Lithium chloride (5 M) 300.0 μl of Polyethylene glycol 2,000 (50 w/v) 680.0 μl of water (100 v/v)
C4	tri-Sodium citrate 0.02 M - di-Sodium hydrogen phosphate 0.1 M pH 6.2 Polyethylene glycol 2,000 15 w/v - 🔍	pH 6.2	300.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M) 10.0 μl of tri-Sodium citrate (2 M) 590.0 μl of water (100 v/v)
C5	Sodium chloride 0.5 M - di-Sodium hydrogen phosphate 0.1 M pH 6.8 Polyethylene glycol 2,000 15 w/v - 🔍	pH 6.8	300.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M) 500.0 μl of water (100 v/v)
C6	BIS-TRIS 0.02 M pH 7 Polyethylene glycol 2,000 15 w/v - 🔍	pH 7	20.0 μl of BIS-TRIS pH 7 (1 M) 300.0 μl of Polyethylene glycol 2,000 (50 w/v) 680.0 μl of water (100 v/v)
D1	Magnesium chloride 0.1 M - HEPES 0.05 M pH 7.5 Polyethylene glycol 2,000 15 w/v - 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 2,000 (50 w/v) 610.0 μl of water (100 v/v)
D2	tris chloride BCC 0.1 M pH 8 Magnesium nitrate 0.3 M - Polyethylene glycol 2,000 20 w/v - 2-methyl-2,4-pentanediol BCC 2 w/v - 🔍	pH 8	22.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 300.0 μl of Magnesium nitrate (1 M) 400.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 177.8 μl of water (100 v/v)
D3	BICINE 0.1 M pH 9 Magnesium chloride 0.3 M - Polyethylene glycol 2,000 25 w/v - Glycerol 15 w/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 187.5 μl of Glycerol (80 w/v) 120.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 2,000 (50 w/v) 92.5 μl of water (100 v/v)
D4	Sodium chloride 0.5 M - di-Sodium hydrogen phosphate 0.2 M pH 6.2 Polyethylene glycol 2,000 30 w/v - 🔍	pH 6.2	600.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium chloride (5 M) 200.0 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M) 100.0 μl of water (100 v/v)
D5	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 2,000 8 w/v - 🔍	pH 4.6	160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v)
D6	tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 200 3 w/v - Polyethylene glycol monomethyl ether 2,000 10 w/v - Glycerol 20 w/v - 🔍	pH 5.6	250.0 μl of Glycerol (80 w/v) 60.0 μl of Polyethylene glycol 200 (50 w/v) 200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 390.0 μl of water (100 v/v)