C6 - Membrane 2 - Screen Contents

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Supplemental Information

Screen Description

Membrane / Membrane 2

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	27
Internal diversity (score mean dist)	0.859

Screen Composition

More information about screen composition chart.

ingredient type	Membrane 2
Buffer	22
Cryoprotectant	2
Organic (non-volatile)	3
Polymer	24
Salt	26

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Membrane 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 0.5 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol monomethyl ether 2,000 12 w/v - 🔍	pH 8.5	240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 210.0 μl of water (100 v/v)
A2	Sodium chloride 0.15 M - tri-Sodium citrate 0.05 M pH 5.6 Polyethylene glycol 3,350 10 w/v - 🔍	pH 5.6	200.0 μl of Polyethylene glycol 3,350 (50 w/v) 150.0 μl of Sodium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 5.6 (1 M) 600.0 μl of water (100 v/v)
A3	tris chloride BCC 0.05 M pH 7.5 Polyethylene glycol 4,000 2 w/v - 🔍	pH 7.5	40.0 μl of Polyethylene glycol 4,000 (50 w/v) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 943.3 μl of water (100 v/v)
A4	Polyethylene glycol 4,000 5 w/v - 🔍	NA	100.0 μl of Polyethylene glycol 4,000 (50 w/v) 900.0 μl of water (100 v/v)
A5	Potassium chloride 0.1 M - Polyethylene glycol 4,000 5 w/v - 🔍	NA	100.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Potassium chloride (5 M) 880.0 μl of water (100 v/v)
A6	MES 0.05 M pH 6.5 Sodium chloride 0.1 M - Polyethylene glycol 4,000 5 w/v - Glycerol 10 w/v - 🔍	pH 6.5	200.0 μl of Glycerol (50 w/v) 50.0 μl of MES pH 6.5 (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 550.0 μl of water (100 v/v)
B1	di-Sodium hydrogen phosphate 0.05 M pH 6.7 Polyethylene glycol 4,000 5 w/v - 🔍	pH 6.7	50.0 μl of di-Sodium hydrogen phosphate pH 6.7 (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 850.0 μl of water (100 v/v)
B2	Sodium chloride 0.5 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 8.5	200.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v)
B3	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol 4,000 12 w/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Lithium sulfate (3 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 626.7 μl of water (100 v/v)
B4	di-Sodium hydrogen phosphate 0.05 M pH 6.8 Polyethylene glycol 4,000 12 w/v - 🔍	pH 6.8	50.0 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 710.0 μl of water (100 v/v)
B5	MOPS 0.05 M pH 7 Potassium chloride 0.5 M - Polyethylene glycol 4,000 12 w/v - Glycerol 20 w/v - 🔍	pH 7	250.0 μl of Glycerol (80 w/v) 50.0 μl of MOPS pH 7 (1 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Potassium chloride (5 M) 360.0 μl of water (100 v/v)
B6	Lithium chloride 0.1 M - tris chloride BCC 0.01 M pH 7.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 7.5	20.0 μl of Lithium chloride (5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 670.0 μl of water (100 v/v)
C1	Sodium chloride 0.5 M - BIS-TRIS 0.1 M pH 7 Polyethylene glycol 4,000 20 w/v - 🔍	pH 7	100.0 μl of BIS-TRIS pH 7 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M)
C2	Sodium chloride 0.5 M - di-Sodium hydrogen phosphate 0.1 M pH 7 Polyethylene glycol 4,000 20 w/v - 🔍	pH 7	100.0 μl of di-Sodium hydrogen phosphate pH 7 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M)
C3	tris chloride BCC 0.05 M pH 7.5 Zinc chloride 0.05 M - Polyethylene glycol 4,000 20 w/v - Zinc acetate 0.15 M - 🔍	pH 7.5	400.0 μl of Polyethylene glycol 4,000 (50 w/v) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 75.0 μl of Zinc acetate (2 M) 50.0 μl of Zinc chloride (1 M) 458.3 μl of water (100 v/v)
C4	TRICINE 0.05 M pH 8 Polyethylene glycol 4,000 22 w/v - 🔍	pH 8	440.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of TRICINE pH 8 (1 M) 510.0 μl of water (100 v/v)
C5	Sodium chloride 0.5 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol 4,000 22 w/v - 🔍	pH 8.5	440.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 10.0 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 30 w/v - 🔍	NA	600.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v)
D1	Magnesium acetate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol monomethyl ether 5,000 10 w/v - 🔍	pH 5.6	50.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 650.0 μl of water (100 v/v)
D2	Magnesium sulfate 0.1 M - Polyethylene glycol 6,000 5 w/v - 🔍	NA	100.0 μl of Magnesium sulfate (1 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 800.0 μl of water (100 v/v)
D3	tris chloride BCC 0.05 M pH 7.5 Zinc chloride 0.05 M - Polyethylene glycol 6,000 10 w/v - Zinc acetate 0.15 M - 🔍	pH 7.5	200.0 μl of Polyethylene glycol 6,000 (50 w/v) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 75.0 μl of Zinc acetate (2 M) 50.0 μl of Zinc chloride (1 M) 658.3 μl of water (100 v/v)
D4	di-Sodium succinate 0.05 M pH 6.5 Polyethylene glycol 6,000 15 w/v - 🔍	pH 6.5	50.0 μl of di-Sodium succinate pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 650.0 μl of water (100 v/v)
D5	Potassium di-hydrogen phosphate 0.025 M - 2-methyl-2,4-pentanediol BCC 10 w/v - Polyethylene glycol 8,000 12 w/v - 🔍	NA	111.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 12.5 μl of Potassium di-hydrogen phosphate (2 M) 636.4 μl of water (100 v/v)
D6	Magnesium acetate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 10,000 8 w/v - 🔍	pH 5.6	50.0 μl of Magnesium acetate (2 M) 160.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 690.0 μl of water (100 v/v)