Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Membrane 2
Barcodes
Membrane 2
No barcodes found.
Screen Structure
Membrane 2
Well Count | 24 |
---|---|
Number of distinct ingredients | 27 |
Internal diversity (score mean dist) | 0.859 |
Screen Composition
ingredient type | Membrane 2 |
---|---|
Buffer | 22 |
Cryoprotectant | 2 |
Organic (non-volatile) | 3 |
Polymer | 24 |
Salt | 26 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8.5 | 240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 210.0 μl of water (100 v/v) |
A2 |
| pH 5.6 | 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 150.0 μl of Sodium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 5.6 (1 M) 600.0 μl of water (100 v/v) |
A3 |
| pH 7.5 | 40.0 μl of Polyethylene glycol 4,000 (50 w/v) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 943.3 μl of water (100 v/v) |
A4 |
| NA | 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 900.0 μl of water (100 v/v) |
A5 |
| NA | 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Potassium chloride (5 M) 880.0 μl of water (100 v/v) |
A6 |
| pH 6.5 | 200.0 μl of Glycerol (50 w/v) 50.0 μl of MES pH 6.5 (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 550.0 μl of water (100 v/v) |
B1 |
| pH 6.7 | 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of di-Sodium hydrogen phosphate pH 6.7 (1 M) 850.0 μl of water (100 v/v) |
B2 |
| pH 8.5 | 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
B3 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Lithium sulfate (3 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 626.7 μl of water (100 v/v) |
B4 |
| pH 6.8 | 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M) 710.0 μl of water (100 v/v) |
B5 |
| pH 7 | 250.0 μl of Glycerol (80 w/v) 50.0 μl of MOPS pH 7 (1 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Potassium chloride (5 M) 360.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 20.0 μl of Lithium chloride (5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 670.0 μl of water (100 v/v) |
C1 |
| pH 7 | 100.0 μl of BIS-TRIS pH 7 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) |
C2 |
| pH 7 | 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 100.0 μl of di-Sodium hydrogen phosphate pH 7 (1 M) |
C3 |
| pH 7.5 | 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 75.0 μl of Zinc acetate (2 M) 50.0 μl of Zinc chloride (1 M) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 458.3 μl of water (100 v/v) |
C4 |
| pH 8 | 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of TRICINE pH 8 (1 M) 510.0 μl of water (100 v/v) |
C5 |
| pH 8.5 | 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 10.0 μl of water (100 v/v) |
C6 |
| NA | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
D1 |
| pH 5.6 | 50.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 650.0 μl of water (100 v/v) |
D2 |
| NA | 100.0 μl of Magnesium sulfate (1 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 800.0 μl of water (100 v/v) |
D3 |
| pH 7.5 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 75.0 μl of Zinc acetate (2 M) 50.0 μl of Zinc chloride (1 M) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 658.3 μl of water (100 v/v) |
D4 |
| pH 6.5 | 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of di-Sodium succinate pH 6.5 (1 M) 650.0 μl of water (100 v/v) |
D5 |
| NA | 111.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 12.5 μl of Potassium di-hydrogen phosphate (2 M) 636.4 μl of water (100 v/v) |
D6 |
| pH 5.6 | 50.0 μl of Magnesium acetate (2 M) 160.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 690.0 μl of water (100 v/v) |