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Supplemental Information

Screen Description

Membrane 2

Membrane / Membrane 2
Created 2009-11-16.

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Membrane 2

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Screen Structure

Membrane 2

Well Count 24
Number of distinct ingredients 27
Internal diversity (score mean dist) 0.859

Screen Composition

2025-08-16T04:27:16.018087 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Membrane 2
Buffer 22
Cryoprotectant 2
Organic (non-volatile) 3
Polymer 24
Salt 26

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Membrane 2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
pH 8.5
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of Sodium chloride (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
210.0 μl of water (100 v/v)
A2
  • Sodium chloride 0.15 M -
  • tri-Sodium citrate 0.05 M pH 5.6
  • Polyethylene glycol 3,350 10 w/v -
pH 5.6
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
150.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 5.6 (1 M)
600.0 μl of water (100 v/v)
A3
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol 4,000 2 w/v -
pH 7.5
40.0 μl of Polyethylene glycol 4,000 (50 w/v)
16.7 μl of tris chloride BCC pH 7.5 (3 M)
943.3 μl of water (100 v/v)
A4
  • Polyethylene glycol 4,000 5 w/v -
NA
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
900.0 μl of water (100 v/v)
A5
  • Potassium chloride 0.1 M -
  • Polyethylene glycol 4,000 5 w/v -
NA
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Potassium chloride (5 M)
880.0 μl of water (100 v/v)
A6
  • MES 0.05 M pH 6.5
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 4,000 5 w/v -
  • Glycerol 10 w/v -
pH 6.5
200.0 μl of Glycerol (50 w/v)
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
550.0 μl of water (100 v/v)
B1
  • di-Sodium hydrogen phosphate 0.05 M pH 6.7
  • Polyethylene glycol 4,000 5 w/v -
pH 6.7
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of di-Sodium hydrogen phosphate pH 6.7 (1 M)
850.0 μl of water (100 v/v)
B2
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 4,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of Sodium chloride (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
B3
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
33.3 μl of Lithium sulfate (3 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
626.7 μl of water (100 v/v)
B4
  • di-Sodium hydrogen phosphate 0.05 M pH 6.8
  • Polyethylene glycol 4,000 12 w/v -
pH 6.8
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M)
710.0 μl of water (100 v/v)
B5
  • MOPS 0.05 M pH 7
  • Potassium chloride 0.5 M -
  • Polyethylene glycol 4,000 12 w/v -
  • Glycerol 20 w/v -
pH 7
250.0 μl of Glycerol (80 w/v)
50.0 μl of MOPS pH 7 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Potassium chloride (5 M)
360.0 μl of water (100 v/v)
B6
  • Lithium chloride 0.1 M -
  • tris chloride BCC 0.01 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
20.0 μl of Lithium chloride (5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
670.0 μl of water (100 v/v)
C1
  • Sodium chloride 0.5 M -
  • BIS-TRIS 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
100.0 μl of BIS-TRIS pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of Sodium chloride (1 M)
C2
  • Sodium chloride 0.5 M -
  • di-Sodium hydrogen phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of Sodium chloride (1 M)
100.0 μl of di-Sodium hydrogen phosphate pH 7 (1 M)
C3
  • tris chloride BCC 0.05 M pH 7.5
  • Zinc chloride 0.05 M -
  • Polyethylene glycol 4,000 20 w/v -
  • Zinc acetate 0.15 M -
pH 7.5
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
75.0 μl of Zinc acetate (2 M)
50.0 μl of Zinc chloride (1 M)
16.7 μl of tris chloride BCC pH 7.5 (3 M)
458.3 μl of water (100 v/v)
C4
  • TRICINE 0.05 M pH 8
  • Polyethylene glycol 4,000 22 w/v -
pH 8
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of TRICINE pH 8 (1 M)
510.0 μl of water (100 v/v)
C5
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 4,000 22 w/v -
pH 8.5
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of Sodium chloride (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
10.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
D1
  • Magnesium acetate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol monomethyl ether 5,000 10 w/v -
pH 5.6
50.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
650.0 μl of water (100 v/v)
D2
  • Magnesium sulfate 0.1 M -
  • Polyethylene glycol 6,000 5 w/v -
NA
100.0 μl of Magnesium sulfate (1 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
800.0 μl of water (100 v/v)
D3
  • tris chloride BCC 0.05 M pH 7.5
  • Zinc chloride 0.05 M -
  • Polyethylene glycol 6,000 10 w/v -
  • Zinc acetate 0.15 M -
pH 7.5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
75.0 μl of Zinc acetate (2 M)
50.0 μl of Zinc chloride (1 M)
16.7 μl of tris chloride BCC pH 7.5 (3 M)
658.3 μl of water (100 v/v)
D4
  • di-Sodium succinate 0.05 M pH 6.5
  • Polyethylene glycol 6,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of di-Sodium succinate pH 6.5 (1 M)
650.0 μl of water (100 v/v)
D5
  • Potassium di-hydrogen phosphate 0.025 M -
  • 2-methyl-2,4-pentanediol BCC 10 w/v -
  • Polyethylene glycol 8,000 12 w/v -
NA
111.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
12.5 μl of Potassium di-hydrogen phosphate (2 M)
636.4 μl of water (100 v/v)
D6
  • Magnesium acetate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 10,000 8 w/v -
pH 5.6
50.0 μl of Magnesium acetate (2 M)
160.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)