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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Membrane / Membrane 3
Created 2009-11-16.
Rigaku Recipe XML
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Screen Structure
Well Count | 24 |
---|---|
Number of distinct ingredients | 24 |
Internal diversity (score mean dist) | 0.894 |
Screen Composition
ingredient type | Membrane 3 |
---|---|
Buffer | 21 |
Cryoprotectant | 1 |
Organic (non-volatile) | 3 |
Organic (volatile) | 3 |
Polymer | 1 |
Salt | 33 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7.5 | 233.3 μl of Ammonium sulfate (3 M) 500.0 μl of Sodium Potassium phosphate pH 7.5 (2 M) 266.7 μl of water (100 v/v) |
A2 |
| pH 6.5 | 333.3 μl of Ammonium sulfate (3 M) 50.0 μl of MES pH 6.5 (1 M) 50.0 μl of Zinc acetate (2 M) 566.7 μl of water (100 v/v) |
A3 |
| pH 7.5 | 400.0 μl of Ammonium sulfate (3 M) 16.7 μl of tris chloride BCC pH 7.5 (3 M) 583.3 μl of water (100 v/v) |
A4 |
| pH 8.5 | 400.0 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
A5 |
| NA | 80.0 μl of 2-Propanol (50 w/v) 20.0 μl of Ammonium acetate (5 M) 466.7 μl of Ammonium sulfate (3 M) 433.3 μl of water (100 v/v) |
A6 |
| NA | 666.7 μl of Ammonium sulfate (3 M) 333.3 μl of water (100 v/v) |
B1 |
| pH 5.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 233.3 μl of water (100 v/v) |
B2 |
| pH 7.5 | 833.3 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 26.7 μl of water (100 v/v) |
B3 |
| NA | 1000.0 μl of Ammonium sulfate (3 M) |
B4 |
| NA | 700.0 μl of Ammonium sulfate (5 M) 300.0 μl of water (100 v/v) |
B5 |
| pH 7.5 | 700.0 μl of Ammonium sulfate (5 M) 50.0 μl of Sodium chloride (5 M) 25.0 μl of Sodium Potassium phosphate pH 7.5 (2 M) 225.0 μl of water (100 v/v) |
B6 |
| pH 7 | 277.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100.0 μl of BIS-TRIS pH 7 (1 M) 622.2 μl of water (100 v/v) |
C1 |
| pH 5.6 | 277.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 300.0 μl of tri-Sodium citrate pH 5.6 (1 M) 422.2 μl of water (100 v/v) |
C2 |
| NA | 33.3 μl of Ammonium sulfate (3 M) 50.0 μl of Glycine (2 M) 500.0 μl of Triethylene glycol (50 w/v) 416.7 μl of water (100 v/v) |
C3 |
| pH 4.6 | 600.0 μl of 2-Propanol (50 w/v) 40.0 μl of Calcium chloride (5 M) 250.0 μl of Glycerol (80 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 10.0 μl of water (100 v/v) |
C4 |
| pH 8 | 50.0 μl of di-Potassium hydrogen phosphate pH 8 (1 M) 950.0 μl of water (100 v/v) |
C5 |
| pH 4.8 | 100.0 μl of tri-Sodium citrate pH 4.8 (1 M) 900.0 μl of water (100 v/v) |
C6 |
| pH 6.5 | 20.0 μl of 1,4-Dioxane (50 w/v) 500.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M) 480.0 μl of water (100 v/v) |
D1 |
| NA | 500.0 μl of tri-Sodium citrate (2 M) 500.0 μl of water (100 v/v) |
D2 |
| NA | 100.0 μl of Lithium chloride (5 M) 500.0 μl of tri-Sodium citrate (2 M) 400.0 μl of water (100 v/v) |
D3 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 300.0 μl of Sodium chloride (5 M) 600.0 μl of water (100 v/v) |
D4 |
| pH 7 | 750.0 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 250.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Lithium sulfate (3 M) 400.0 μl of water (100 v/v) |
D6 |
| NA | 400.0 μl of Sodium chloride (5 M) 14.3 μl of Sodium formate (7 M) 585.7 μl of water (100 v/v) |