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Supplemental Information

Screen Description

Membrane 3

Membrane / Membrane 3
Created 2009-11-16.

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Membrane 3

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Membrane 3

    Screen Structure

    Membrane 3

    Well Count 24
    Number of distinct ingredients 24
    Internal diversity (score mean dist) 0.894

    Screen Composition

    More information about screen composition chart.
    2025-06-15T21:43:37.890743 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Membrane 3
    Buffer 21
    Cryoprotectant 1
    Organic (non-volatile) 3
    Organic (volatile) 3
    Polymer 1
    Salt 33

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Membrane 3 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium Potassium phosphate 1 M pH 7.5
    • Ammonium sulfate 0.7 M -
    		pH 7.5
    233 μl of Ammonium sulfate (3 M)
    500 μl of Sodium Potassium phosphate pH 7.5 (2 M)
    266 μl of water (100 v/v)
    A2
    • Zinc acetate 0.1 M -
    • MES 0.05 M pH 6.5
    • Ammonium sulfate 1 M -
    		pH 6.5
    50 μl of MES pH 6.5 (1 M)
    50 μl of Zinc acetate (2 M)
    333 μl of Ammonium sulfate (3 M)
    566 μl of water (100 v/v)
    A3
    • tris chloride BCC 0.05 M pH 7.5
    • Ammonium sulfate 1.2 M -
    		pH 7.5
    16 μl of tris chloride BCC pH 7.5 (3 M)
    400 μl of Ammonium sulfate (3 M)
    583 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.2 M -
    		pH 8.5
    400 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    A5
    • Ammonium acetate 0.1 M -
    • 2-Propanol 4 w/v -
    • Ammonium sulfate 1.4 M -
    		NA
    20 μl of Ammonium acetate (5 M)
    80 μl of 2-Propanol (50 w/v)
    466 μl of Ammonium sulfate (3 M)
    433 μl of water (100 v/v)
    A6
    • Ammonium sulfate 2 M -
    		NA
    666 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    B2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 2 w/v -
    • Ammonium sulfate 2.5 M -
    		pH 7.5
    833 μl of Ammonium sulfate (3 M)
    40 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    26 μl of water (100 v/v)
    B3
    • Ammonium sulfate 3 M -
    		NA
    1000 μl of Ammonium sulfate (3 M)
    B4
    • Ammonium sulfate 3.5 M -
    		NA
    700 μl of Ammonium sulfate (5 M)
    300 μl of water (100 v/v)
    B5
    • Sodium chloride 0.25 M -
    • Sodium Potassium phosphate 0.05 M pH 7.5
    • Ammonium sulfate 3.5 M -
    		pH 7.5
    700 μl of Ammonium sulfate (5 M)
    25 μl of Sodium Potassium phosphate pH 7.5 (2 M)
    50 μl of Sodium chloride (5 M)
    225 μl of water (100 v/v)
    B6
    • BIS-TRIS 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 25 w/v -
    		pH 7
    100 μl of BIS-TRIS pH 7 (1 M)
    277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    622 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.3 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 25 w/v -
    		pH 5.6
    300 μl of tri-Sodium citrate pH 5.6 (1 M)
    277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    422 μl of water (100 v/v)
    C2
    • Glycine 0.1 M -
    • Ammonium sulfate 0.1 M -
    • Triethylene glycol 25 w/v -
    		NA
    50 μl of Glycine (2 M)
    33 μl of Ammonium sulfate (3 M)
    500 μl of Triethylene glycol (50 w/v)
    416 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.2 M -
    • 2-Propanol 30 w/v -
    • Glycerol 20 w/v -
    		pH 4.6
    600 μl of 2-Propanol (50 w/v)
    40 μl of Calcium chloride (5 M)
    250 μl of Glycerol (80 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    10 μl of water (100 v/v)
    C4
    • di-Potassium hydrogen phosphate 0.05 M pH 8
    		pH 8
    50 μl of di-Potassium hydrogen phosphate pH 8 (1 M)
    950 μl of water (100 v/v)
    C5
    • tri-Sodium citrate 0.1 M pH 4.8
    		pH 4.8
    100 μl of tri-Sodium citrate pH 4.8 (1 M)
    900 μl of water (100 v/v)
    C6
    • 1,4-Dioxane 1 w/v -
    • di-Potassium hydrogen phosphate 1 M pH 6.5
    		pH 6.5
    20 μl of 1,4-Dioxane (50 w/v)
    500 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    480 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 1 M -
    		NA
    500 μl of tri-Sodium citrate (2 M)
    500 μl of water (100 v/v)
    D2
    • Lithium chloride 0.5 M -
    • tri-Sodium citrate 1 M -
    		NA
    500 μl of tri-Sodium citrate (2 M)
    100 μl of Lithium chloride (5 M)
    400 μl of water (100 v/v)
    D3
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 1.5 M -
    		pH 4.6
    300 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of water (100 v/v)
    D4
    • di-Potassium hydrogen phosphate 1.5 M pH 7
    		pH 7
    750 μl of di-Potassium hydrogen phosphate pH 7 (2 M)
    250 μl of water (100 v/v)
    D5
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    		pH 7.5
    500 μl of Lithium sulfate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    D6
    • Sodium formate 0.1 M -
    • Sodium chloride 2 M -
    		NA
    14 μl of Sodium formate (7 M)
    400 μl of Sodium chloride (5 M)
    585 μl of water (100 v/v)