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Supplemental Information

Screen Description

Membrane 3

Membrane / Membrane 3
Created 2009-11-16.

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Membrane 3

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Membrane 3

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Screen Structure

Membrane 3

Well Count 24
Number of distinct ingredients 24
Internal diversity (score mean dist) 0.894

Screen Composition

More information about screen composition chart.
2025-08-16T11:21:04.213130 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Membrane 3
Buffer 21
Cryoprotectant 1
Organic (non-volatile) 3
Organic (volatile) 3
Polymer 1
Salt 33

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Membrane 3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium Potassium phosphate 1 M pH 7.5
  • Ammonium sulfate 0.7 M -
pH 7.5
233.3 μl of Ammonium sulfate (3 M)
500.0 μl of Sodium Potassium phosphate pH 7.5 (2 M)
266.7 μl of water (100 v/v)
A2
  • Zinc acetate 0.1 M -
  • MES 0.05 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
333.3 μl of Ammonium sulfate (3 M)
50.0 μl of MES pH 6.5 (1 M)
50.0 μl of Zinc acetate (2 M)
566.7 μl of water (100 v/v)
A3
  • tris chloride BCC 0.05 M pH 7.5
  • Ammonium sulfate 1.2 M -
pH 7.5
400.0 μl of Ammonium sulfate (3 M)
16.7 μl of tris chloride BCC pH 7.5 (3 M)
583.3 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.2 M -
pH 8.5
400.0 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
A5
  • Ammonium acetate 0.1 M -
  • 2-Propanol 4 w/v -
  • Ammonium sulfate 1.4 M -
NA
80.0 μl of 2-Propanol (50 w/v)
20.0 μl of Ammonium acetate (5 M)
466.7 μl of Ammonium sulfate (3 M)
433.3 μl of water (100 v/v)
A6
  • Ammonium sulfate 2 M -
NA
666.7 μl of Ammonium sulfate (3 M)
333.3 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
233.3 μl of water (100 v/v)
B2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 5,000 2 w/v -
  • Ammonium sulfate 2.5 M -
pH 7.5
833.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
26.7 μl of water (100 v/v)
B3
  • Ammonium sulfate 3 M -
NA
1000.0 μl of Ammonium sulfate (3 M)
B4
  • Ammonium sulfate 3.5 M -
NA
700.0 μl of Ammonium sulfate (5 M)
300.0 μl of water (100 v/v)
B5
  • Sodium chloride 0.25 M -
  • Sodium Potassium phosphate 0.05 M pH 7.5
  • Ammonium sulfate 3.5 M -
pH 7.5
700.0 μl of Ammonium sulfate (5 M)
25.0 μl of Sodium Potassium phosphate pH 7.5 (2 M)
50.0 μl of Sodium chloride (5 M)
225.0 μl of water (100 v/v)
B6
  • BIS-TRIS 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 25 w/v -
pH 7
277.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of BIS-TRIS pH 7 (1 M)
622.2 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.3 M pH 5.6
  • 2-methyl-2,4-pentanediol BCC 25 w/v -
pH 5.6
277.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
300.0 μl of tri-Sodium citrate pH 5.6 (1 M)
422.2 μl of water (100 v/v)
C2
  • Glycine 0.1 M -
  • Ammonium sulfate 0.1 M -
  • Triethylene glycol 25 w/v -
NA
33.3 μl of Ammonium sulfate (3 M)
50.0 μl of Glycine (2 M)
500.0 μl of Triethylene glycol (50 w/v)
416.7 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.2 M -
  • 2-Propanol 30 w/v -
  • Glycerol 20 w/v -
pH 4.6
600.0 μl of 2-Propanol (50 w/v)
40.0 μl of Calcium chloride (5 M)
250.0 μl of Glycerol (80 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
10.0 μl of water (100 v/v)
C4
  • di-Potassium hydrogen phosphate 0.05 M pH 8
pH 8
50.0 μl of di-Potassium hydrogen phosphate pH 8 (1 M)
950.0 μl of water (100 v/v)
C5
  • tri-Sodium citrate 0.1 M pH 4.8
pH 4.8
100.0 μl of tri-Sodium citrate pH 4.8 (1 M)
900.0 μl of water (100 v/v)
C6
  • 1,4-Dioxane 1 w/v -
  • di-Potassium hydrogen phosphate 1 M pH 6.5
pH 6.5
20.0 μl of 1,4-Dioxane (50 w/v)
500.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
480.0 μl of water (100 v/v)
D1
  • tri-Sodium citrate 1 M -
NA
500.0 μl of tri-Sodium citrate (2 M)
500.0 μl of water (100 v/v)
D2
  • Lithium chloride 0.5 M -
  • tri-Sodium citrate 1 M -
NA
100.0 μl of Lithium chloride (5 M)
500.0 μl of tri-Sodium citrate (2 M)
400.0 μl of water (100 v/v)
D3
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 1.5 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
D4
  • di-Potassium hydrogen phosphate 1.5 M pH 7
pH 7
750.0 μl of di-Potassium hydrogen phosphate pH 7 (2 M)
250.0 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Lithium sulfate (3 M)
400.0 μl of water (100 v/v)
D6
  • Sodium formate 0.1 M -
  • Sodium chloride 2 M -
NA
400.0 μl of Sodium chloride (5 M)
14.3 μl of Sodium formate (7 M)
585.7 μl of water (100 v/v)