Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Membrane HTS

Membrane / Membrane HTS
Created 2009-11-16.

Export

Barcodes

Membrane HTS

    Screen Structure

    Membrane HTS

    Well Count 96
    Number of distinct ingredients 52
    Internal diversity (score mean dist) 0.948

    Screen Composition

    2025-06-15T02:22:07.797046 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Membrane HTS
    Buffer 62
    Cryoprotectant 7
    Organic (non-volatile) 17
    Organic (volatile) 4
    Polymer 50
    Salt 85

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Membrane HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    • Glycerol 15 w/v -
    • Polyethylene glycol 400 15 w/v -
    pH 7.5
    300 μl of Glycerol (50 w/v)
    40 μl of Calcium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (50 w/v)
    260 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium chloride 0.02 M -
    • Polyethylene glycol 400 20 w/v -
    • Sodium chloride 0.1 M -
    pH 5.6
    400 μl of Polyethylene glycol 400 (50 w/v)
    100 μl of Sodium chloride (1 M)
    8 μl of Magnesium chloride (2.5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    392 μl of water (100 v/v)
    A3
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 4.6
    • Polyethylene glycol 400 25 w/v -
    pH 4.6
    25 μl of Magnesium acetate (2 M)
    50 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Polyethylene glycol 400 (50 w/v)
    425 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.05 M pH 8.5
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol 400 30 w/v -
    • Sodium sulfate 0.05 M -
    pH 8.5
    50 μl of Sodium sulfate (1 M)
    600 μl of Polyethylene glycol 400 (50 w/v)
    16 μl of Lithium sulfate (3 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    283 μl of water (100 v/v)
    A5
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 48 w/v -
    pH 7.5
    640 μl of Polyethylene glycol 400 (75 w/v)
    200 μl of Calcium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    60 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.01 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 20 w/v -
    pH 7.5
    3 μl of tris chloride BCC pH 7.5 (3 M)
    400 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    596 μl of water (100 v/v)
    A7
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol monomethyl ether 550 30 w/v -
    pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    310 μl of water (100 v/v)
    A8
    • Polyethylene glycol 600 35 w/v -
    NA
    700 μl of Polyethylene glycol 600 (50 w/v)
    300 μl of water (100 v/v)
    A9
    • TRICINE 0.1 M pH 8
    • Sodium chloride 0.35 M -
    • Polyethylene glycol 1,000 28 w/v -
    • Glycerol 10 w/v -
    pH 8
    200 μl of Glycerol (50 w/v)
    70 μl of Sodium chloride (5 M)
    100 μl of TRICINE pH 8 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    70 μl of water (100 v/v)
    A10
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 1,500 10 w/v -
    • Ethanol 5 w/v -
    NA
    75 μl of Ethanol (66 w/v)
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Sodium chloride (1 M)
    40 μl of Magnesium chloride (2.5 M)
    584 μl of water (100 v/v)
    A11
    • Polyethylene glycol 1,500 30 w/v -
    NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A12
    • Polyethylene glycol 2,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 2,000 (50 w/v)
    900 μl of water (100 v/v)
    B1
    • Magnesium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 2,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Magnesium chloride (2.5 M)
    500 μl of water (100 v/v)
    B2
    • Polyethylene glycol 2,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    700 μl of water (100 v/v)
    B3
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 2,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of Lithium chloride (1 M)
    600 μl of water (100 v/v)
    B4
    • tri-Sodium citrate 0.02 M -
    • di-Sodium hydrogen phosphate 0.1 M pH 6.2
    • Polyethylene glycol 2,000 15 w/v -
    pH 6.2
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    10 μl of tri-Sodium citrate (2 M)
    100 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M)
    590 μl of water (100 v/v)
    B5
    • Sodium chloride 0.5 M -
    • di-Sodium hydrogen phosphate 0.1 M pH 6.8
    • Polyethylene glycol 2,000 15 w/v -
    pH 6.8
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M)
    500 μl of Sodium chloride (1 M)
    100 μl of water (100 v/v)
    B6
    • BIS-TRIS 0.02 M pH 7
    • Polyethylene glycol 2,000 15 w/v -
    pH 7
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    20 μl of BIS-TRIS pH 7 (1 M)
    680 μl of water (100 v/v)
    B7
    • Magnesium chloride 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 2,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    50 μl of HEPES pH 7.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    610 μl of water (100 v/v)
    B8
    • tris chloride BCC 0.1 M pH 8
    • Magnesium nitrate 0.3 M -
    • Polyethylene glycol 2,000 20 w/v -
    • 2-methyl-2,4-pentanediol BCC 2 w/v -
    pH 8
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    22 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Magnesium nitrate (1 M)
    177 μl of water (100 v/v)
    B9
    • BICINE 0.1 M pH 9
    • Magnesium chloride 0.3 M -
    • Polyethylene glycol 2,000 25 w/v -
    • Glycerol 15 w/v -
    pH 9
    500 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    187 μl of Glycerol (80 w/v)
    120 μl of Magnesium chloride (2.5 M)
    92 μl of water (100 v/v)
    B10
    • Sodium chloride 0.5 M -
    • di-Sodium hydrogen phosphate 0.2 M pH 6.2
    • Polyethylene glycol 2,000 30 w/v -
    pH 6.2
    600 μl of Polyethylene glycol 2,000 (50 w/v)
    200 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M)
    100 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    B11
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 8 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    740 μl of water (100 v/v)
    B12
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 200 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Glycerol 20 w/v -
    pH 5.6
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    60 μl of Polyethylene glycol 200 (50 w/v)
    250 μl of Glycerol (80 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    390 μl of water (100 v/v)
    C1
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    pH 8.5
    100 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    610 μl of water (100 v/v)
    C2
    • Sodium chloride 0.15 M -
    • tri-Sodium citrate 0.05 M pH 5.6
    • Polyethylene glycol 3,350 10 w/v -
    pH 5.6
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    150 μl of Sodium chloride (1 M)
    50 μl of tri-Sodium citrate pH 5.6 (1 M)
    600 μl of water (100 v/v)
    C3
    • tris chloride BCC 0.05 M pH 7.5
    • Polyethylene glycol 4,000 2 w/v -
    pH 7.5
    40 μl of Polyethylene glycol 4,000 (50 w/v)
    16 μl of tris chloride BCC pH 7.5 (3 M)
    943 μl of water (100 v/v)
    C4
    • Polyethylene glycol 4,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    900 μl of water (100 v/v)
    C5
    • Potassium chloride 0.1 M -
    • Polyethylene glycol 4,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Potassium chloride (5 M)
    880 μl of water (100 v/v)
    C6
    • MES 0.05 M pH 6.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 5 w/v -
    • Glycerol 10 w/v -
    pH 6.5
    200 μl of Glycerol (50 w/v)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of MES pH 6.5 (1 M)
    100 μl of Sodium chloride (1 M)
    550 μl of water (100 v/v)
    C7
    • di-Sodium hydrogen phosphate 0.05 M pH 6.7
    • Polyethylene glycol 4,000 5 w/v -
    pH 6.7
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of di-Sodium hydrogen phosphate pH 6.7 (1 M)
    850 μl of water (100 v/v)
    C8
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 8.5
    100 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    C9
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of ADA pH 6.5 (1 M)
    33 μl of Lithium sulfate (3 M)
    626 μl of water (100 v/v)
    C10
    • di-Sodium hydrogen phosphate 0.05 M pH 6.8
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.8
    50 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    710 μl of water (100 v/v)
    C11
    • MOPS 0.05 M pH 7
    • Potassium chloride 0.5 M -
    • Polyethylene glycol 4,000 12 w/v -
    • Glycerol 20 w/v -
    pH 7
    50 μl of MOPS pH 7 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Potassium chloride (5 M)
    250 μl of Glycerol (80 w/v)
    360 μl of water (100 v/v)
    C12
    • Lithium chloride 0.1 M -
    • tris chloride BCC 0.01 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    10 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Lithium chloride (5 M)
    670 μl of water (100 v/v)
    D1
    • Sodium chloride 0.5 M -
    • BIS-TRIS 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    100 μl of BIS-TRIS pH 7 (1 M)
    100 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    D2
    • Sodium chloride 0.5 M -
    • di-Sodium hydrogen phosphate 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    100 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of di-Sodium hydrogen phosphate pH 7 (1 M)
    400 μl of water (100 v/v)
    D3
    • tris chloride BCC 0.05 M pH 7.5
    • Zinc chloride 0.05 M -
    • Polyethylene glycol 4,000 20 w/v -
    • Zinc acetate 0.15 M -
    pH 7.5
    50 μl of Zinc chloride (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    16 μl of tris chloride BCC pH 7.5 (3 M)
    75 μl of Zinc acetate (2 M)
    458 μl of water (100 v/v)
    D4
    • TRICINE 0.05 M pH 8
    • Polyethylene glycol 4,000 22 w/v -
    pH 8
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of TRICINE pH 8 (1 M)
    510 μl of water (100 v/v)
    D5
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 8.5
    100 μl of Sodium chloride (5 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    410 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    D7
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol monomethyl ether 5,000 10 w/v -
    pH 5.6
    50 μl of Magnesium acetate (2 M)
    200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    650 μl of water (100 v/v)
    D8
    • Magnesium sulfate 0.1 M -
    • Polyethylene glycol 6,000 5 w/v -
    NA
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Magnesium sulfate (1 M)
    800 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.05 M pH 7.5
    • Zinc chloride 0.05 M -
    • Polyethylene glycol 6,000 10 w/v -
    • Zinc acetate 0.15 M -
    pH 7.5
    50 μl of Zinc chloride (1 M)
    16 μl of tris chloride BCC pH 7.5 (3 M)
    75 μl of Zinc acetate (2 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    658 μl of water (100 v/v)
    D10
    • di-Sodium succinate 0.05 M pH 6.5
    • Polyethylene glycol 6,000 15 w/v -
    pH 6.5
    50 μl of di-Sodium succinate pH 6.5 (1 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    650 μl of water (100 v/v)
    D11
    • Potassium di-hydrogen phosphate 0.025 M -
    • 2-methyl-2,4-pentanediol BCC 10 w/v -
    • Polyethylene glycol 8,000 12 w/v -
    NA
    25 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
    111 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    623 μl of water (100 v/v)
    D12
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 10,000 8 w/v -
    pH 5.6
    50 μl of Magnesium acetate (2 M)
    160 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    690 μl of water (100 v/v)
    E1
    • Sodium Potassium phosphate 1 M pH 7.5
    • Ammonium sulfate 0.7 M -
    pH 7.5
    233 μl of Ammonium sulfate (3 M)
    500 μl of Sodium Potassium phosphate pH 7.5 (2 M)
    266 μl of water (100 v/v)
    E2
    • Zinc acetate 0.1 M -
    • MES 0.05 M pH 6.5
    • Ammonium sulfate 1 M -
    pH 6.5
    333 μl of Ammonium sulfate (3 M)
    50 μl of MES pH 6.5 (1 M)
    50 μl of Zinc acetate (2 M)
    566 μl of water (100 v/v)
    E3
    • tris chloride BCC 0.05 M pH 7.5
    • Ammonium sulfate 1.2 M -
    pH 7.5
    16 μl of tris chloride BCC pH 7.5 (3 M)
    240 μl of Ammonium sulfate (5 M)
    743 μl of water (100 v/v)
    E4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    240 μl of Ammonium sulfate (5 M)
    660 μl of water (100 v/v)
    E5
    • Ammonium acetate 0.1 M -
    • 2-Propanol 4 w/v -
    • Ammonium sulfate 1.4 M -
    NA
    20 μl of Ammonium acetate (5 M)
    80 μl of 2-Propanol (50 w/v)
    280 μl of Ammonium sulfate (5 M)
    620 μl of water (100 v/v)
    E6
    • Ammonium sulfate 2 M -
    NA
    400 μl of Ammonium sulfate (5 M)
    600 μl of water (100 v/v)
    E7
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    pH 5.6
    400 μl of Ammonium sulfate (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 2 w/v -
    • Ammonium sulfate 2.5 M -
    pH 7.5
    40 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Ammonium sulfate (5 M)
    360 μl of water (100 v/v)
    E9
    • Ammonium sulfate 3 M -
    NA
    600 μl of Ammonium sulfate (5 M)
    400 μl of water (100 v/v)
    E10
    • Ammonium sulfate 3.5 M -
    NA
    700 μl of Ammonium sulfate (5 M)
    300 μl of water (100 v/v)
    E11
    • Sodium chloride 0.25 M -
    • Sodium Potassium phosphate 0.05 M pH 7.5
    • Ammonium sulfate 3.5 M -
    pH 7.5
    50 μl of Sodium chloride (5 M)
    25 μl of Sodium Potassium phosphate pH 7.5 (2 M)
    700 μl of Ammonium sulfate (5 M)
    225 μl of water (100 v/v)
    E12
    • BIS-TRIS 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 25 w/v -
    pH 7
    100 μl of BIS-TRIS pH 7 (1 M)
    277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    622 μl of water (100 v/v)
    F1
    • tri-Sodium citrate 0.3 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 25 w/v -
    pH 5.6
    277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    300 μl of tri-Sodium citrate pH 5.6 (1 M)
    422 μl of water (100 v/v)
    F2
    • Glycine 0.1 M -
    • Ammonium sulfate 0.1 M -
    • Triethylene glycol 25 w/v -
    NA
    50 μl of Glycine (2 M)
    33 μl of Ammonium sulfate (3 M)
    500 μl of Triethylene glycol (50 w/v)
    416 μl of water (100 v/v)
    F3
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.2 M -
    • 2-Propanol 30 w/v -
    • Glycerol 20 w/v -
    pH 4.6
    40 μl of Calcium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of 2-Propanol (50 w/v)
    250 μl of Glycerol (80 w/v)
    10 μl of water (100 v/v)
    F4
    • di-Potassium hydrogen phosphate 0.05 M pH 8
    pH 8
    50 μl of di-Potassium hydrogen phosphate pH 8 (1 M)
    950 μl of water (100 v/v)
    F5
    • tri-Sodium citrate 0.1 M pH 4.8
    pH 4.8
    100 μl of tri-Sodium citrate pH 4.8 (1 M)
    900 μl of water (100 v/v)
    F6
    • 1,4-Dioxane 1 w/v -
    • di-Potassium hydrogen phosphate 1 M pH 6.5
    pH 6.5
    20 μl of 1,4-Dioxane (50 w/v)
    500 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    480 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 1 M -
    NA
    500 μl of tri-Sodium citrate (2 M)
    500 μl of water (100 v/v)
    F8
    • Lithium chloride 0.5 M -
    • tri-Sodium citrate 1 M -
    NA
    500 μl of tri-Sodium citrate (2 M)
    100 μl of Lithium chloride (5 M)
    400 μl of water (100 v/v)
    F9
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 1.5 M -
    pH 4.6
    300 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of water (100 v/v)
    F10
    • di-Potassium hydrogen phosphate 1.5 M pH 7
    pH 7
    750 μl of di-Potassium hydrogen phosphate pH 7 (2 M)
    250 μl of water (100 v/v)
    F11
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Lithium sulfate (3 M)
    400 μl of water (100 v/v)
    F12
    • Sodium formate 0.1 M -
    • Sodium chloride 2 M -
    NA
    400 μl of Sodium chloride (5 M)
    14 μl of Sodium formate (7 M)
    585 μl of water (100 v/v)
    G1NA
    G2NA
    G3NA
    G4NA
    G5NA
    G6NA
    G7NA
    G8NA
    G9NA
    G10NA
    G11NA
    G12NA
    H1NA
    H2NA
    H3NA
    H4NA
    H5NA
    H6NA
    H7NA
    H8NA
    H9NA
    H10NA
    H11NA
    H12NA