Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Membrane HTS
Barcodes
Membrane HTS
Screen Structure
Membrane HTS
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.948 |
Screen Composition
ingredient type | Membrane HTS |
---|---|
Buffer | 62 |
Cryoprotectant | 7 |
Organic (non-volatile) | 17 |
Organic (volatile) | 4 |
Polymer | 50 |
Salt | 85 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7.5 | 300 μl of Glycerol (50 w/v) 40 μl of Calcium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of Polyethylene glycol 400 (50 w/v) 260 μl of water (100 v/v) |
A2 |
| pH 5.6 | 400 μl of Polyethylene glycol 400 (50 w/v) 100 μl of Sodium chloride (1 M) 8 μl of Magnesium chloride (2.5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 392 μl of water (100 v/v) |
A3 |
| pH 4.6 | 25 μl of Magnesium acetate (2 M) 50 μl of Sodium acetate pH 4.6 (1 M) 500 μl of Polyethylene glycol 400 (50 w/v) 425 μl of water (100 v/v) |
A4 |
| pH 8.5 | 50 μl of Sodium sulfate (1 M) 600 μl of Polyethylene glycol 400 (50 w/v) 16 μl of Lithium sulfate (3 M) 50 μl of tris chloride BCC pH 8.5 (1 M) 283 μl of water (100 v/v) |
A5 |
| pH 7.5 | 640 μl of Polyethylene glycol 400 (75 w/v) 200 μl of Calcium chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 60 μl of water (100 v/v) |
A6 |
| pH 7.5 | 3 μl of tris chloride BCC pH 7.5 (3 M) 400 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 596 μl of water (100 v/v) |
A7 |
| pH 8.5 | 50 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 40 μl of Magnesium chloride (2.5 M) 310 μl of water (100 v/v) |
A8 |
| NA | 700 μl of Polyethylene glycol 600 (50 w/v) 300 μl of water (100 v/v) |
A9 |
| pH 8 | 200 μl of Glycerol (50 w/v) 70 μl of Sodium chloride (5 M) 100 μl of TRICINE pH 8 (1 M) 560 μl of Polyethylene glycol 1,000 (50 w/v) 70 μl of water (100 v/v) |
A10 |
| NA | 75 μl of Ethanol (66 w/v) 200 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of Sodium chloride (1 M) 40 μl of Magnesium chloride (2.5 M) 584 μl of water (100 v/v) |
A11 |
| NA | 600 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v) |
A12 |
| NA | 100 μl of Polyethylene glycol 2,000 (50 w/v) 900 μl of water (100 v/v) |
B1 |
| pH 8.5 | 200 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Magnesium chloride (2.5 M) 500 μl of water (100 v/v) |
B2 |
| NA | 300 μl of Polyethylene glycol 2,000 (50 w/v) 700 μl of water (100 v/v) |
B3 |
| NA | 300 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of Lithium chloride (1 M) 600 μl of water (100 v/v) |
B4 |
| pH 6.2 | 300 μl of Polyethylene glycol 2,000 (50 w/v) 10 μl of tri-Sodium citrate (2 M) 100 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M) 590 μl of water (100 v/v) |
B5 |
| pH 6.8 | 300 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M) 500 μl of Sodium chloride (1 M) 100 μl of water (100 v/v) |
B6 |
| pH 7 | 300 μl of Polyethylene glycol 2,000 (50 w/v) 20 μl of BIS-TRIS pH 7 (1 M) 680 μl of water (100 v/v) |
B7 |
| pH 7.5 | 300 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of HEPES pH 7.5 (1 M) 40 μl of Magnesium chloride (2.5 M) 610 μl of water (100 v/v) |
B8 |
| pH 8 | 400 μl of Polyethylene glycol 2,000 (50 w/v) 22 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of Magnesium nitrate (1 M) 177 μl of water (100 v/v) |
B9 |
| pH 9 | 500 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 187 μl of Glycerol (80 w/v) 120 μl of Magnesium chloride (2.5 M) 92 μl of water (100 v/v) |
B10 |
| pH 6.2 | 600 μl of Polyethylene glycol 2,000 (50 w/v) 200 μl of di-Sodium hydrogen phosphate pH 6.2 (1 M) 100 μl of Sodium chloride (5 M) 100 μl of water (100 v/v) |
B11 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 740 μl of water (100 v/v) |
B12 |
| pH 5.6 | 200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 60 μl of Polyethylene glycol 200 (50 w/v) 250 μl of Glycerol (80 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 390 μl of water (100 v/v) |
C1 |
| pH 8.5 | 100 μl of Sodium chloride (5 M) 240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50 μl of tris chloride BCC pH 8.5 (1 M) 610 μl of water (100 v/v) |
C2 |
| pH 5.6 | 200 μl of Polyethylene glycol 3,350 (50 w/v) 150 μl of Sodium chloride (1 M) 50 μl of tri-Sodium citrate pH 5.6 (1 M) 600 μl of water (100 v/v) |
C3 |
| pH 7.5 | 40 μl of Polyethylene glycol 4,000 (50 w/v) 16 μl of tris chloride BCC pH 7.5 (3 M) 943 μl of water (100 v/v) |
C4 |
| NA | 100 μl of Polyethylene glycol 4,000 (50 w/v) 900 μl of water (100 v/v) |
C5 |
| NA | 100 μl of Polyethylene glycol 4,000 (50 w/v) 20 μl of Potassium chloride (5 M) 880 μl of water (100 v/v) |
C6 |
| pH 6.5 | 200 μl of Glycerol (50 w/v) 100 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of MES pH 6.5 (1 M) 100 μl of Sodium chloride (1 M) 550 μl of water (100 v/v) |
C7 |
| pH 6.7 | 100 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of di-Sodium hydrogen phosphate pH 6.7 (1 M) 850 μl of water (100 v/v) |
C8 |
| pH 8.5 | 100 μl of Sodium chloride (5 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of tris chloride BCC pH 8.5 (1 M) 650 μl of water (100 v/v) |
C9 |
| pH 6.5 | 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of ADA pH 6.5 (1 M) 33 μl of Lithium sulfate (3 M) 626 μl of water (100 v/v) |
C10 |
| pH 6.8 | 50 μl of di-Sodium hydrogen phosphate pH 6.8 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 710 μl of water (100 v/v) |
C11 |
| pH 7 | 50 μl of MOPS pH 7 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Potassium chloride (5 M) 250 μl of Glycerol (80 w/v) 360 μl of water (100 v/v) |
C12 |
| pH 7.5 | 10 μl of tris chloride BCC pH 7.5 (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 20 μl of Lithium chloride (5 M) 670 μl of water (100 v/v) |
D1 |
| pH 7 | 100 μl of BIS-TRIS pH 7 (1 M) 100 μl of Sodium chloride (5 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of water (100 v/v) |
D2 |
| pH 7 | 100 μl of Sodium chloride (5 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of di-Sodium hydrogen phosphate pH 7 (1 M) 400 μl of water (100 v/v) |
D3 |
| pH 7.5 | 50 μl of Zinc chloride (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 16 μl of tris chloride BCC pH 7.5 (3 M) 75 μl of Zinc acetate (2 M) 458 μl of water (100 v/v) |
D4 |
| pH 8 | 440 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of TRICINE pH 8 (1 M) 510 μl of water (100 v/v) |
D5 |
| pH 8.5 | 100 μl of Sodium chloride (5 M) 440 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of tris chloride BCC pH 8.5 (1 M) 410 μl of water (100 v/v) |
D6 |
| NA | 600 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of water (100 v/v) |
D7 |
| pH 5.6 | 50 μl of Magnesium acetate (2 M) 200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 650 μl of water (100 v/v) |
D8 |
| NA | 100 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Magnesium sulfate (1 M) 800 μl of water (100 v/v) |
D9 |
| pH 7.5 | 50 μl of Zinc chloride (1 M) 16 μl of tris chloride BCC pH 7.5 (3 M) 75 μl of Zinc acetate (2 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 658 μl of water (100 v/v) |
D10 |
| pH 6.5 | 50 μl of di-Sodium succinate pH 6.5 (1 M) 300 μl of Polyethylene glycol 6,000 (50 w/v) 650 μl of water (100 v/v) |
D11 |
| NA | 25 μl of Potassium di-hydrogen phosphate pH 7 (1 M) 111 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 240 μl of Polyethylene glycol 8,000 (50 w/v) 623 μl of water (100 v/v) |
D12 |
| pH 5.6 | 50 μl of Magnesium acetate (2 M) 160 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 690 μl of water (100 v/v) |
E1 |
| pH 7.5 | 233 μl of Ammonium sulfate (3 M) 500 μl of Sodium Potassium phosphate pH 7.5 (2 M) 266 μl of water (100 v/v) |
E2 |
| pH 6.5 | 333 μl of Ammonium sulfate (3 M) 50 μl of MES pH 6.5 (1 M) 50 μl of Zinc acetate (2 M) 566 μl of water (100 v/v) |
E3 |
| pH 7.5 | 16 μl of tris chloride BCC pH 7.5 (3 M) 240 μl of Ammonium sulfate (5 M) 743 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 240 μl of Ammonium sulfate (5 M) 660 μl of water (100 v/v) |
E5 |
| NA | 20 μl of Ammonium acetate (5 M) 80 μl of 2-Propanol (50 w/v) 280 μl of Ammonium sulfate (5 M) 620 μl of water (100 v/v) |
E6 |
| NA | 400 μl of Ammonium sulfate (5 M) 600 μl of water (100 v/v) |
E7 |
| pH 5.6 | 400 μl of Ammonium sulfate (5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 500 μl of water (100 v/v) |
E8 |
| pH 7.5 | 40 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of Ammonium sulfate (5 M) 360 μl of water (100 v/v) |
E9 |
| NA | 600 μl of Ammonium sulfate (5 M) 400 μl of water (100 v/v) |
E10 |
| NA | 700 μl of Ammonium sulfate (5 M) 300 μl of water (100 v/v) |
E11 |
| pH 7.5 | 50 μl of Sodium chloride (5 M) 25 μl of Sodium Potassium phosphate pH 7.5 (2 M) 700 μl of Ammonium sulfate (5 M) 225 μl of water (100 v/v) |
E12 |
| pH 7 | 100 μl of BIS-TRIS pH 7 (1 M) 277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 622 μl of water (100 v/v) |
F1 |
| pH 5.6 | 277 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 300 μl of tri-Sodium citrate pH 5.6 (1 M) 422 μl of water (100 v/v) |
F2 |
| NA | 50 μl of Glycine (2 M) 33 μl of Ammonium sulfate (3 M) 500 μl of Triethylene glycol (50 w/v) 416 μl of water (100 v/v) |
F3 |
| pH 4.6 | 40 μl of Calcium chloride (5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 600 μl of 2-Propanol (50 w/v) 250 μl of Glycerol (80 w/v) 10 μl of water (100 v/v) |
F4 |
| pH 8 | 50 μl of di-Potassium hydrogen phosphate pH 8 (1 M) 950 μl of water (100 v/v) |
F5 |
| pH 4.8 | 100 μl of tri-Sodium citrate pH 4.8 (1 M) 900 μl of water (100 v/v) |
F6 |
| pH 6.5 | 20 μl of 1,4-Dioxane (50 w/v) 500 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M) 480 μl of water (100 v/v) |
F7 |
| NA | 500 μl of tri-Sodium citrate (2 M) 500 μl of water (100 v/v) |
F8 |
| NA | 500 μl of tri-Sodium citrate (2 M) 100 μl of Lithium chloride (5 M) 400 μl of water (100 v/v) |
F9 |
| pH 4.6 | 300 μl of Sodium chloride (5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 600 μl of water (100 v/v) |
F10 |
| pH 7 | 750 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 250 μl of water (100 v/v) |
F11 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 500 μl of Lithium sulfate (3 M) 400 μl of water (100 v/v) |
F12 |
| NA | 400 μl of Sodium chloride (5 M) 14 μl of Sodium formate (7 M) 585 μl of water (100 v/v) |
G1 | NA | ||
G2 | NA | ||
G3 | NA | ||
G4 | NA | ||
G5 | NA | ||
G6 | NA | ||
G7 | NA | ||
G8 | NA | ||
G9 | NA | ||
G10 | NA | ||
G11 | NA | ||
G12 | NA | ||
H1 | NA | ||
H2 | NA | ||
H3 | NA | ||
H4 | NA | ||
H5 | NA | ||
H6 | NA | ||
H7 | NA | ||
H8 | NA | ||
H9 | NA | ||
H10 | NA | ||
H11 | NA | ||
H12 | NA |