C6 - Nuc-Pro 2 - Screen Contents

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Supplemental Information

Screen Description

Nuc-Pro / Nuc-Pro 2

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.923

Screen Composition

More information about screen composition chart.

ingredient type	Nuc-Pro 2
Buffer	30
Cryoprotectant	3
Organic (non-volatile)	5
Organic (volatile)	1
Polymer	24
Salt	28

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Nuc-Pro 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 4,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.6 Potassium chloride 0.1 M - 2-Propanol 10 v/v - 🔍	pH 5.6	100.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Potassium chloride (4 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 375.0 μl of water (100 v/v)
A2	Polyethylene glycol 4,000 20 w/v - MOPS 0.05 M pH 7 Sodium chloride 0.1 M - 🔍	pH 7	50.0 μl of MOPS pH 7 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 450.0 μl of water (100 v/v)
A3	Polyethylene glycol 4,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v)
A4	Polyethylene glycol 4,000 24 w/v - di-Sodium succinate 0.05 M pH 5.5 Magnesium chloride 0.06 M - Sodium chloride 0.3 M - 🔍	pH 5.5	16.7 μl of di-Sodium succinate pH 5.5 (3 M) 24.0 μl of Magnesium chloride (2.5 M) 480.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of Sodium chloride (1 M) 179.3 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 25 w/v - tri-Sodium citrate 0.05 M pH 5 Ammonium sulfate 0.05 M - 🔍	pH 5	50.0 μl of Ammonium sulfate (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tri-Sodium citrate pH 5 (1 M) 400.0 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 30 w/v - MES 0.05 M pH 6.5 Magnesium acetate 0.08 M - 🔍	pH 6.5	80.0 μl of Magnesium acetate (1 M) 50.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 270.0 μl of water (100 v/v)
B1	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.05 M pH 8.5 Ammonium chloride 0.15 M - Calcium chloride 0.01 M - 🔍	pH 8.5	30.0 μl of Ammonium chloride (5 M) 10.0 μl of Calcium chloride (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 310.0 μl of water (100 v/v)
B2	Polyethylene glycol 4,000 32 w/v - tris chloride BCC 0.1 M pH 8.5 Glycerol 5 v/v - 🔍	pH 8.5	50.0 μl of Glycerol (100 v/v) 640.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 210.0 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 36 w/v - Sodium acetate 0.05 M pH 5 🔍	pH 5	720.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 5 (1 M) 230.0 μl of water (100 v/v)
B4	Polyethylene glycol 6,000 5 w/v - BIS-TRIS 0.02 M pH 6 di-Ammonium hydrogen citrate 0.06 M - Magnesium chloride 0.02 M - 🔍	pH 6	20.0 μl of BIS-TRIS pH 6 (1 M) 60.0 μl of di-Ammonium hydrogen citrate (1 M) 8.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 812.0 μl of water (100 v/v)
B5	Polyethylene glycol 6,000 10 w/v - tri-Sodium citrate 0.05 M pH 4 Sodium chloride 0.1 M - 🔍	pH 4	200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 4 (1 M) 650.0 μl of water (100 v/v)
B6	Polyethylene glycol 6,000 10 w/v - HEPES 0.05 M pH 7 Ammonium acetate 0.2 M - Magnesium acetate 0.15 M - 🔍	pH 7	200.0 μl of Ammonium acetate (1 M) 50.0 μl of HEPES pH 7 (1 M) 150.0 μl of Magnesium acetate (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of water (100 v/v)
C1	Polyethylene glycol 6,000 15 w/v - tris chloride BCC 0.01 M pH 7.5 🔍	pH 7.5	300.0 μl of Polyethylene glycol 6,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 690.0 μl of water (100 v/v)
C2	Polyethylene glycol 6,000 18 w/v - Sodium acetate 0.05 M pH 5 🔍	pH 5	360.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium acetate pH 5 (1 M) 590.0 μl of water (100 v/v)
C3	Polyethylene glycol 6,000 20 w/v - BIS-TRIS propane 0.05 M pH 7 2-methyl-2,4-pentanediol BCC 7 v/v - 2-Methyl-2-propanol 5 v/v - 🔍	pH 7	140.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 100.0 μl of 2-Methyl-2-propanol (50 v/v) 50.0 μl of BIS-TRIS propane pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 310.0 μl of water (100 v/v)
C4	Polyethylene glycol 6,000 20 w/v - di-Potassium L-Malate 0.05 M pH 5 Calcium chloride 0.03 M - 🔍	pH 5	30.0 μl of Calcium chloride (1 M) 50.0 μl of di-Potassium L-Malate pH 5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 520.0 μl of water (100 v/v)
C5	Polyethylene glycol 8,000 5 w/v - HEPES 0.05 M pH 7.5 Magnesium chloride 0.02 M - 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 842.0 μl of water (100 v/v)
C6	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 8 Glycerol 10 v/v - Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M - 🔍	pH 8	200.0 μl of Glycerol (50 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M) 499.0 μl of water (100 v/v)
D1	Polyethylene glycol 8,000 10 w/v - MES 0.05 M pH 6.5 Potassium chloride 0.2 M - Magnesium acetate 0.1 M - 🔍	pH 6.5	100.0 μl of Magnesium acetate (1 M) 50.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of Potassium chloride (1 M) 450.0 μl of water (100 v/v)
D2	Polyethylene glycol 8,000 15 w/v - BIS-TRIS propane 0.05 M pH 6.8 Ammonium sulfate 0.1 M - Glycerol 10 v/v - 🔍	pH 6.8	100.0 μl of Ammonium sulfate (1 M) 50.0 μl of BIS-TRIS propane pH 6.8 (1 M) 200.0 μl of Glycerol (50 v/v) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 350.0 μl of water (100 v/v)
D3	Polyethylene glycol 8,000 15 w/v - MES 0.1 M pH 6.5 Calcium acetate 0.2 M - 🔍	pH 6.5	200.0 μl of Calcium acetate (1 M) 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 18 w/v - tris chloride BCC 0.1 M pH 8 Magnesium formate 0.2 M - 🔍	pH 8	200.0 μl of Magnesium formate (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 340.0 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.01 M pH 7.5 Calcium chloride 0.01 M - 🔍	pH 7.5	10.0 μl of Calcium chloride (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 580.0 μl of water (100 v/v)
D6	Polyethylene glycol 20,000 15 w/v - MES 0.1 M pH 6.5 Manganese (II) chloride 0.08 M - 🔍	pH 6.5	80.0 μl of Manganese (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 20,000 (50 w/v) 520.0 μl of water (100 v/v)