Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Nuc-Pro 2

Nuc-Pro / Nuc-Pro 2
Created 2009-11-16.

Export

Barcodes

Nuc-Pro 2

No barcodes found.

Screen Structure

Nuc-Pro 2

Well Count 24
Number of distinct ingredients 31
Internal diversity (score mean dist) 0.923

Screen Composition

2025-08-16T14:27:59.074040 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Nuc-Pro 2
Buffer 30
Cryoprotectant 3
Organic (non-volatile) 5
Organic (volatile) 1
Polymer 24
Salt 28

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Nuc-Pro 2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 4,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Potassium chloride 0.1 M -
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
375.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 4,000 20 w/v -
  • MOPS 0.05 M pH 7
  • Sodium chloride 0.1 M -
pH 7
50.0 μl of MOPS pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
450.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 4,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 4,000 24 w/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Magnesium chloride 0.06 M -
  • Sodium chloride 0.3 M -
pH 5.5
24.0 μl of Magnesium chloride (2.5 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of Sodium chloride (1 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
179.3 μl of water (100 v/v)
A5
  • Polyethylene glycol 4,000 25 w/v -
  • tri-Sodium citrate 0.05 M pH 5
  • Ammonium sulfate 0.05 M -
pH 5
50.0 μl of Ammonium sulfate (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tri-Sodium citrate pH 5 (1 M)
400.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 4,000 30 w/v -
  • MES 0.05 M pH 6.5
  • Magnesium acetate 0.08 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
270.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.05 M pH 8.5
  • Ammonium chloride 0.15 M -
  • Calcium chloride 0.01 M -
pH 8.5
30.0 μl of Ammonium chloride (5 M)
10.0 μl of Calcium chloride (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
310.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 4,000 32 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 5 v/v -
pH 8.5
50.0 μl of Glycerol (100 v/v)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
210.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 4,000 36 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
720.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 5 (1 M)
230.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 6,000 5 w/v -
  • BIS-TRIS 0.02 M pH 6
  • di-Ammonium hydrogen citrate 0.06 M -
  • Magnesium chloride 0.02 M -
pH 6
20.0 μl of BIS-TRIS pH 6 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
60.0 μl of di-Ammonium hydrogen citrate (1 M)
812.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 6,000 10 w/v -
  • tri-Sodium citrate 0.05 M pH 4
  • Sodium chloride 0.1 M -
pH 4
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 4 (1 M)
650.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.05 M pH 7
  • Ammonium acetate 0.2 M -
  • Magnesium acetate 0.15 M -
pH 7
200.0 μl of Ammonium acetate (1 M)
50.0 μl of HEPES pH 7 (1 M)
150.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 6,000 15 w/v -
  • tris chloride BCC 0.01 M pH 7.5
pH 7.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
690.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 6,000 18 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
360.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium acetate pH 5 (1 M)
590.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 6,000 20 w/v -
  • BIS-TRIS propane 0.05 M pH 7
  • 2-methyl-2,4-pentanediol BCC 7 v/v -
  • 2-Methyl-2-propanol 5 v/v -
pH 7
100.0 μl of 2-Methyl-2-propanol (50 v/v)
140.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
50.0 μl of BIS-TRIS propane pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
310.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 6,000 20 w/v -
  • di-Potassium L-Malate 0.05 M pH 5
  • Calcium chloride 0.03 M -
pH 5
30.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of di-Potassium L-Malate pH 5 (1 M)
520.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 8,000 5 w/v -
  • HEPES 0.05 M pH 7.5
  • Magnesium chloride 0.02 M -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
842.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Glycerol 10 v/v -
  • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
pH 8
200.0 μl of Glycerol (50 v/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
499.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 8,000 10 w/v -
  • MES 0.05 M pH 6.5
  • Potassium chloride 0.2 M -
  • Magnesium acetate 0.1 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Potassium chloride (1 M)
450.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 8,000 15 w/v -
  • BIS-TRIS propane 0.05 M pH 6.8
  • Ammonium sulfate 0.1 M -
  • Glycerol 10 v/v -
pH 6.8
100.0 μl of Ammonium sulfate (1 M)
50.0 μl of BIS-TRIS propane pH 6.8 (1 M)
200.0 μl of Glycerol (50 v/v)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
350.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 8,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 8,000 18 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium formate 0.2 M -
pH 8
200.0 μl of Magnesium formate (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.01 M pH 7.5
  • Calcium chloride 0.01 M -
pH 7.5
10.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
580.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 20,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Manganese (II) chloride 0.08 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Manganese (II) chloride (1 M)
300.0 μl of Polyethylene glycol 20,000 (50 w/v)
520.0 μl of water (100 v/v)