Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Nuc-Pro 2
Barcodes
Nuc-Pro 2
No barcodes found.
Screen Structure
Nuc-Pro 2
Well Count | 24 |
---|---|
Number of distinct ingredients | 31 |
Internal diversity (score mean dist) | 0.923 |
Screen Composition
ingredient type | Nuc-Pro 2 |
---|---|
Buffer | 30 |
Cryoprotectant | 3 |
Organic (non-volatile) | 5 |
Organic (volatile) | 1 |
Polymer | 24 |
Salt | 28 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.6 | 100.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Potassium chloride (4 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 375.0 μl of water (100 v/v) |
A2 |
| pH 7 | 50.0 μl of MOPS pH 7 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 450.0 μl of water (100 v/v) |
A3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v) |
A4 |
| pH 5.5 | 24.0 μl of Magnesium chloride (2.5 M) 480.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of Sodium chloride (1 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 179.3 μl of water (100 v/v) |
A5 |
| pH 5 | 50.0 μl of Ammonium sulfate (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tri-Sodium citrate pH 5 (1 M) 400.0 μl of water (100 v/v) |
A6 |
| pH 6.5 | 50.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium acetate (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 270.0 μl of water (100 v/v) |
B1 |
| pH 8.5 | 30.0 μl of Ammonium chloride (5 M) 10.0 μl of Calcium chloride (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 310.0 μl of water (100 v/v) |
B2 |
| pH 8.5 | 50.0 μl of Glycerol (100 v/v) 640.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 210.0 μl of water (100 v/v) |
B3 |
| pH 5 | 720.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 5 (1 M) 230.0 μl of water (100 v/v) |
B4 |
| pH 6 | 20.0 μl of BIS-TRIS pH 6 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 60.0 μl of di-Ammonium hydrogen citrate (1 M) 812.0 μl of water (100 v/v) |
B5 |
| pH 4 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 4 (1 M) 650.0 μl of water (100 v/v) |
B6 |
| pH 7 | 200.0 μl of Ammonium acetate (1 M) 50.0 μl of HEPES pH 7 (1 M) 150.0 μl of Magnesium acetate (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of water (100 v/v) |
C1 |
| pH 7.5 | 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 690.0 μl of water (100 v/v) |
C2 |
| pH 5 | 360.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium acetate pH 5 (1 M) 590.0 μl of water (100 v/v) |
C3 |
| pH 7 | 100.0 μl of 2-Methyl-2-propanol (50 v/v) 140.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 50.0 μl of BIS-TRIS propane pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 310.0 μl of water (100 v/v) |
C4 |
| pH 5 | 30.0 μl of Calcium chloride (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of di-Potassium L-Malate pH 5 (1 M) 520.0 μl of water (100 v/v) |
C5 |
| pH 7.5 | 50.0 μl of HEPES pH 7.5 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 842.0 μl of water (100 v/v) |
C6 |
| pH 8 | 200.0 μl of Glycerol (50 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 499.0 μl of water (100 v/v) |
D1 |
| pH 6.5 | 50.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of Potassium chloride (1 M) 450.0 μl of water (100 v/v) |
D2 |
| pH 6.8 | 100.0 μl of Ammonium sulfate (1 M) 50.0 μl of BIS-TRIS propane pH 6.8 (1 M) 200.0 μl of Glycerol (50 v/v) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 350.0 μl of water (100 v/v) |
D3 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v) |
D4 |
| pH 8 | 200.0 μl of Magnesium formate (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 340.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 10.0 μl of Calcium chloride (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 10.0 μl of tris chloride BCC pH 7.5 (1 M) 580.0 μl of water (100 v/v) |
D6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Manganese (II) chloride (1 M) 300.0 μl of Polyethylene glycol 20,000 (50 w/v) 520.0 μl of water (100 v/v) |