C6 - Nuc-Pro 3 - Screen Contents

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Supplemental Information

Screen Description

Nuc-Pro / Nuc-Pro 3

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	25
Internal diversity (score mean dist)	0.890

Screen Composition

More information about screen composition chart.

ingredient type	Nuc-Pro 3
Buffer	27
Cryoprotectant	2
Organic (non-volatile)	5
Organic (volatile)	1
Polymer	3
Salt	39

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Nuc-Pro 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ammonium sulfate 1.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 2 w/v - 🔍	pH 7.5	240.0 μl of Ammonium sulfate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol 400 (50 w/v) 620.0 μl of water (100 v/v)
A2	Calcium chloride 0.02 M - Glycine 0.1 M pH 8 🔍	pH 8	20.0 μl of Calcium chloride (1 M) 100.0 μl of Glycine pH 8 (1 M) 880.0 μl of water (100 v/v)
A3	Lithium chloride 2 M - MES 0.05 M pH 6 Calcium acetate 0.2 M - Cobalt (II) chloride 0.001 M - 🔍	pH 6	200.0 μl of Calcium acetate (1 M) 1.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Lithium chloride (5 M) 50.0 μl of MES pH 6 (1 M) 349.0 μl of water (100 v/v)
A4	Lithium sulfate 0.6 M - MES 0.05 M pH 6 Magnesium chloride 0.01 M - 🔍	pH 6	600.0 μl of Lithium sulfate (1 M) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6 (1 M) 346.0 μl of water (100 v/v)
A5	Lithium sulfate 1 M - Polyethylene glycol 400 8 w/v - HEPES 0.05 M pH 7.5 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Lithium sulfate (2.5 M) 160.0 μl of Polyethylene glycol 400 (50 w/v) 390.0 μl of water (100 v/v)
A6	Lithium sulfate 1 M - tri-Sodium citrate 0.05 M - 2-Propanol 3 w/v - 🔍	NA	60.0 μl of 2-Propanol (50 w/v) 400.0 μl of Lithium sulfate (2.5 M) 25.0 μl of tri-Sodium citrate (2 M) 515.0 μl of water (100 v/v)
B1	Lithium sulfate 1.2 M - MES 0.05 M pH 6.5 Magnesium chloride 0.03 M - 🔍	pH 6.5	480.0 μl of Lithium sulfate (2.5 M) 12.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 458.0 μl of water (100 v/v)
B2	Lithium sulfate 1.2 M - MES 0.05 M pH 6.5 Magnesium chloride 0.05 M - Cobalt (II) chloride 0.002 M - 🔍	pH 6.5	2.0 μl of Cobalt (II) chloride (1 M) 480.0 μl of Lithium sulfate (2.5 M) 20.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 448.0 μl of water (100 v/v)
B3	Lithium sulfate 1.5 M - tris chloride BCC 0.05 M pH 8.5 Glycerol 5 w/v - 🔍	pH 8.5	100.0 μl of Glycerol (50 w/v) 600.0 μl of Lithium sulfate (2.5 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v)
B4	Lithium sulfate 1.6 M - Polyethylene glycol 1,000 2 w/v - HEPES 0.05 M pH 7.5 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 640.0 μl of Lithium sulfate (2.5 M) 40.0 μl of Polyethylene glycol 1,000 (50 w/v) 270.0 μl of water (100 v/v)
B5	Lithium sulfate 1.7 M - HEPES 0.05 M pH 7 Magnesium sulfate 0.05 M - 🔍	pH 7	50.0 μl of HEPES pH 7 (1 M) 680.0 μl of Lithium sulfate (2.5 M) 50.0 μl of Magnesium sulfate (1 M) 220.0 μl of water (100 v/v)
B6	Lithium sulfate 1.7 M - Glycerol 10 w/v - 🔍	NA	200.0 μl of Glycerol (50 w/v) 680.0 μl of Lithium sulfate (2.5 M) 120.0 μl of water (100 v/v)
C1	Lithium sulfate 2 M - 2-methyl-2,4-pentanediol BCC 3 w/v - 🔍	NA	33.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 800.0 μl of Lithium sulfate (2.5 M) 166.7 μl of water (100 v/v)
C2	Magnesium chloride 0.005 M - MES 0.05 M pH 6.5 Cobalt (II) chloride 0.0025 M - 🔍	pH 6.5	2.5 μl of Cobalt (II) chloride (1 M) 2.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 945.5 μl of water (100 v/v)
C3	Magnesium chloride 0.04 M - MES 0.05 M pH 6 🔍	pH 6	16.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6 (1 M) 934.0 μl of water (100 v/v)
C4	Magnesium chloride 0.08 M - HEPES 0.05 M pH 7.5 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 32.0 μl of Magnesium chloride (2.5 M) 918.0 μl of water (100 v/v)
C5	Sodium chloride 0.2 M - BIS-TRIS propane 0.05 M pH 7 🔍	pH 7	50.0 μl of BIS-TRIS propane pH 7 (1 M) 200.0 μl of Sodium chloride (1 M) 750.0 μl of water (100 v/v)
C6	Sodium chloride 0.4 M - BIS-TRIS propane 0.05 M pH 6.8 🔍	pH 6.8	50.0 μl of BIS-TRIS propane pH 6.8 (1 M) 400.0 μl of Sodium chloride (1 M) 550.0 μl of water (100 v/v)
D1	Sodium chloride 0.6 M - MES 0.05 M pH 6 Ammonium acetate 0.1 M - Magnesium sulfate 0.005 M - 🔍	pH 6	100.0 μl of Ammonium acetate (1 M) 5.0 μl of Magnesium sulfate (1 M) 50.0 μl of MES pH 6 (1 M) 120.0 μl of Sodium chloride (5 M) 725.0 μl of water (100 v/v)
D2	Sodium chloride 2.5 M - tris chloride BCC 0.05 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Sodium chloride (5 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 370.0 μl of water (100 v/v)
D3	Sodium formate 1.8 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	360.0 μl of Sodium formate (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 540.0 μl of water (100 v/v)
D4	Sodium formate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium formate (5 M) 500.0 μl of water (100 v/v)
D5	di-Sodium hydrogen phosphate 0.1 M - Sodium chloride 0.08 M - 🔍	NA	20.0 μl of di-Sodium hydrogen phosphate (5 M) 80.0 μl of Sodium chloride (1 M) 900.0 μl of water (100 v/v)
D6	di-Sodium tartrate 1 M - tris chloride BCC 0.05 M pH 7.5 Magnesium chloride 0.03 M - 🔍	pH 7.5	500.0 μl of di-Sodium tartrate (2 M) 12.0 μl of Magnesium chloride (2.5 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 438.0 μl of water (100 v/v)