Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Nuc-Pro / Nuc-Pro 3
Created 2009-11-16.
Rigaku Recipe XML
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Barcodes
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Screen Structure
Well Count | 24 |
---|---|
Number of distinct ingredients | 25 |
Internal diversity (score mean dist) | 0.890 |
Screen Composition
ingredient type | Nuc-Pro 3 |
---|---|
Buffer | 27 |
Cryoprotectant | 2 |
Organic (non-volatile) | 5 |
Organic (volatile) | 1 |
Polymer | 3 |
Salt | 39 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7.5 | 240.0 μl of Ammonium sulfate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol 400 (50 w/v) 620.0 μl of water (100 v/v) |
A2 |
| pH 8 | 20.0 μl of Calcium chloride (1 M) 100.0 μl of Glycine pH 8 (1 M) 880.0 μl of water (100 v/v) |
A3 |
| pH 6 | 200.0 μl of Calcium acetate (1 M) 1.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Lithium chloride (5 M) 50.0 μl of MES pH 6 (1 M) 349.0 μl of water (100 v/v) |
A4 |
| pH 6 | 600.0 μl of Lithium sulfate (1 M) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6 (1 M) 346.0 μl of water (100 v/v) |
A5 |
| pH 7.5 | 50.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Lithium sulfate (2.5 M) 160.0 μl of Polyethylene glycol 400 (50 w/v) 390.0 μl of water (100 v/v) |
A6 |
| NA | 60.0 μl of 2-Propanol (50 w/v) 400.0 μl of Lithium sulfate (2.5 M) 25.0 μl of tri-Sodium citrate (2 M) 515.0 μl of water (100 v/v) |
B1 |
| pH 6.5 | 480.0 μl of Lithium sulfate (2.5 M) 12.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 458.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 2.0 μl of Cobalt (II) chloride (1 M) 480.0 μl of Lithium sulfate (2.5 M) 20.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 448.0 μl of water (100 v/v) |
B3 |
| pH 8.5 | 100.0 μl of Glycerol (50 w/v) 600.0 μl of Lithium sulfate (2.5 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 50.0 μl of HEPES pH 7.5 (1 M) 640.0 μl of Lithium sulfate (2.5 M) 40.0 μl of Polyethylene glycol 1,000 (50 w/v) 270.0 μl of water (100 v/v) |
B5 |
| pH 7 | 50.0 μl of HEPES pH 7 (1 M) 680.0 μl of Lithium sulfate (2.5 M) 50.0 μl of Magnesium sulfate (1 M) 220.0 μl of water (100 v/v) |
B6 |
| NA | 200.0 μl of Glycerol (50 w/v) 680.0 μl of Lithium sulfate (2.5 M) 120.0 μl of water (100 v/v) |
C1 |
| NA | 33.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 800.0 μl of Lithium sulfate (2.5 M) 166.7 μl of water (100 v/v) |
C2 |
| pH 6.5 | 2.5 μl of Cobalt (II) chloride (1 M) 2.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 945.5 μl of water (100 v/v) |
C3 |
| pH 6 | 16.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6 (1 M) 934.0 μl of water (100 v/v) |
C4 |
| pH 7.5 | 50.0 μl of HEPES pH 7.5 (1 M) 32.0 μl of Magnesium chloride (2.5 M) 918.0 μl of water (100 v/v) |
C5 |
| pH 7 | 50.0 μl of BIS-TRIS propane pH 7 (1 M) 200.0 μl of Sodium chloride (1 M) 750.0 μl of water (100 v/v) |
C6 |
| pH 6.8 | 50.0 μl of BIS-TRIS propane pH 6.8 (1 M) 400.0 μl of Sodium chloride (1 M) 550.0 μl of water (100 v/v) |
D1 |
| pH 6 | 100.0 μl of Ammonium acetate (1 M) 5.0 μl of Magnesium sulfate (1 M) 50.0 μl of MES pH 6 (1 M) 120.0 μl of Sodium chloride (5 M) 725.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Sodium chloride (5 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 370.0 μl of water (100 v/v) |
D3 |
| pH 8 | 360.0 μl of Sodium formate (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 540.0 μl of water (100 v/v) |
D4 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium formate (5 M) 500.0 μl of water (100 v/v) |
D5 |
| NA | 20.0 μl of di-Sodium hydrogen phosphate (5 M) 80.0 μl of Sodium chloride (1 M) 900.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 500.0 μl of di-Sodium tartrate (2 M) 12.0 μl of Magnesium chloride (2.5 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 438.0 μl of water (100 v/v) |