C6 - Screen Contents

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Supplemental Information

Screen Description

Nuc-Pro 3

Nuc-Pro / Nuc-Pro 3

Created 2009-11-16.

Export

Nuc-Pro 3

Barcodes

Nuc-Pro 3

Screen Structure

Nuc-Pro 3

Well Count	24
Number of distinct ingredients	25
Internal diversity (score mean dist)	0.890

Screen Composition

More information about screen composition chart.

ingredient type	Nuc-Pro 3
Buffer	27
Cryoprotectant	2
Organic (non-volatile)	5
Organic (volatile)	1
Polymer	3
Salt	39

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Nuc-Pro 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ammonium sulfate 1.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 2 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 240 μl of Ammonium sulfate (5 M) 40 μl of Polyethylene glycol 400 (50 w/v) 620 μl of water (100 v/v)
A2	Calcium chloride 0.02 M - Glycine 0.1 M pH 8 🔍	pH 8	100 μl of Glycine pH 8 (1 M) 20 μl of Calcium chloride (1 M) 880 μl of water (100 v/v)
A3	Lithium chloride 2 M - MES 0.05 M pH 6 Calcium acetate 0.2 M - Cobalt (II) chloride 0.001 M - 🔍	pH 6	400 μl of Lithium chloride (5 M) 200 μl of Calcium acetate (1 M) 50 μl of MES pH 6 (1 M) 1 μl of Cobalt (II) chloride (1 M) 349 μl of water (100 v/v)
A4	Lithium sulfate 0.6 M - MES 0.05 M pH 6 Magnesium chloride 0.01 M - 🔍	pH 6	4 μl of Magnesium chloride (2.5 M) 600 μl of Lithium sulfate (1 M) 50 μl of MES pH 6 (1 M) 346 μl of water (100 v/v)
A5	Lithium sulfate 1 M - Polyethylene glycol 400 8 w/v - HEPES 0.05 M pH 7.5 🔍	pH 7.5	50 μl of HEPES pH 7.5 (1 M) 400 μl of Lithium sulfate (2.5 M) 160 μl of Polyethylene glycol 400 (50 w/v) 390 μl of water (100 v/v)
A6	Lithium sulfate 1 M - tri-Sodium citrate 0.05 M - 2-Propanol 3 w/v - 🔍	NA	25 μl of tri-Sodium citrate (2 M) 400 μl of Lithium sulfate (2.5 M) 60 μl of 2-Propanol (50 w/v) 515 μl of water (100 v/v)
B1	Lithium sulfate 1.2 M - MES 0.05 M pH 6.5 Magnesium chloride 0.03 M - 🔍	pH 6.5	12 μl of Magnesium chloride (2.5 M) 50 μl of MES pH 6.5 (1 M) 480 μl of Lithium sulfate (2.5 M) 458 μl of water (100 v/v)
B2	Lithium sulfate 1.2 M - MES 0.05 M pH 6.5 Magnesium chloride 0.05 M - Cobalt (II) chloride 0.002 M - 🔍	pH 6.5	20 μl of Magnesium chloride (2.5 M) 50 μl of MES pH 6.5 (1 M) 480 μl of Lithium sulfate (2.5 M) 2 μl of Cobalt (II) chloride (1 M) 448 μl of water (100 v/v)
B3	Lithium sulfate 1.5 M - tris chloride BCC 0.05 M pH 8.5 Glycerol 5 w/v - 🔍	pH 8.5	50 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Lithium sulfate (2.5 M) 100 μl of Glycerol (50 w/v) 250 μl of water (100 v/v)
B4	Lithium sulfate 1.6 M - Polyethylene glycol 1,000 2 w/v - HEPES 0.05 M pH 7.5 🔍	pH 7.5	40 μl of Polyethylene glycol 1,000 (50 w/v) 50 μl of HEPES pH 7.5 (1 M) 640 μl of Lithium sulfate (2.5 M) 270 μl of water (100 v/v)
B5	Lithium sulfate 1.7 M - HEPES 0.05 M pH 7 Magnesium sulfate 0.05 M - 🔍	pH 7	50 μl of HEPES pH 7 (1 M) 680 μl of Lithium sulfate (2.5 M) 50 μl of Magnesium sulfate (1 M) 220 μl of water (100 v/v)
B6	Lithium sulfate 1.7 M - Glycerol 10 w/v - 🔍	NA	680 μl of Lithium sulfate (2.5 M) 200 μl of Glycerol (50 w/v) 120 μl of water (100 v/v)
C1	Lithium sulfate 2 M - 2-methyl-2,4-pentanediol BCC 3 w/v - 🔍	NA	800 μl of Lithium sulfate (2.5 M) 33 μl of 2-methyl-2,4-pentanediol BCC (90 w/v) 166 μl of water (100 v/v)
C2	Magnesium chloride 0.005 M - MES 0.05 M pH 6.5 Cobalt (II) chloride 0.0025 M - 🔍	pH 6.5	2 μl of Magnesium chloride (2.5 M) 50 μl of MES pH 6.5 (1 M) 2 μl of Cobalt (II) chloride (1 M) 945 μl of water (100 v/v)
C3	Magnesium chloride 0.04 M - MES 0.05 M pH 6 🔍	pH 6	16 μl of Magnesium chloride (2.5 M) 50 μl of MES pH 6 (1 M) 934 μl of water (100 v/v)
C4	Magnesium chloride 0.08 M - HEPES 0.05 M pH 7.5 🔍	pH 7.5	32 μl of Magnesium chloride (2.5 M) 50 μl of HEPES pH 7.5 (1 M) 918 μl of water (100 v/v)
C5	Sodium chloride 0.2 M - BIS-TRIS propane 0.05 M pH 7 🔍	pH 7	50 μl of BIS-TRIS propane pH 7 (1 M) 200 μl of Sodium chloride (1 M) 750 μl of water (100 v/v)
C6	Sodium chloride 0.4 M - BIS-TRIS propane 0.05 M pH 6.8 🔍	pH 6.8	50 μl of BIS-TRIS propane pH 6.8 (1 M) 400 μl of Sodium chloride (1 M) 550 μl of water (100 v/v)
D1	Sodium chloride 0.6 M - MES 0.05 M pH 6 Ammonium acetate 0.1 M - Magnesium sulfate 0.005 M - 🔍	pH 6	120 μl of Sodium chloride (5 M) 100 μl of Ammonium acetate (1 M) 50 μl of MES pH 6 (1 M) 5 μl of Magnesium sulfate (1 M) 725 μl of water (100 v/v)
D2	Sodium chloride 2.5 M - tris chloride BCC 0.05 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	80 μl of Magnesium chloride (2.5 M) 500 μl of Sodium chloride (5 M) 50 μl of tris chloride BCC pH 7.5 (1 M) 370 μl of water (100 v/v)
D3	Sodium formate 1.8 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	360 μl of Sodium formate (5 M) 100 μl of tris chloride BCC pH 8 (1 M) 540 μl of water (100 v/v)
D4	Sodium formate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	400 μl of Sodium formate (5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 500 μl of water (100 v/v)
D5	di-Sodium hydrogen phosphate 0.1 M - Sodium chloride 0.08 M - 🔍	NA	20 μl of di-Sodium hydrogen phosphate (5 M) 80 μl of Sodium chloride (1 M) 900 μl of water (100 v/v)
D6	di-Sodium tartrate 1 M - tris chloride BCC 0.05 M pH 7.5 Magnesium chloride 0.03 M - 🔍	pH 7.5	12 μl of Magnesium chloride (2.5 M) 50 μl of tris chloride BCC pH 7.5 (1 M) 500 μl of di-Sodium tartrate (2 M) 438 μl of water (100 v/v)