C6 - Screen Contents

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Supplemental Information

Screen Description

Nuc-Pro 4

Nuc-Pro / Nuc-Pro 4

Created 2009-11-16.

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Nuc-Pro 4

Barcodes

Nuc-Pro 4

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Screen Structure

Nuc-Pro 4

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.897

Screen Composition

More information about screen composition chart.

ingredient type	Nuc-Pro 4
Buffer	30
Cryoprotectant	1
Organic (non-volatile)	15
Organic (volatile)	9
Polymer	1
Salt	35

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Nuc-Pro 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	1,6-Hexanediol 10 w/v - MES 0.05 M pH 6.5 Magnesium chloride 0.02 M - 🔍	pH 6.5	200.0 μl of 1,6-Hexanediol (50 w/v) 50.0 μl of MES pH 6.5 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 742.0 μl of water (100 v/v)
A2	1,6-Hexanediol 20 w/v - HEPES 0.05 M pH 7 Ammonium chloride 0.05 M - Magnesium chloride 0.01 M - 🔍	pH 7	400.0 μl of 1,6-Hexanediol (50 w/v) 10.0 μl of Ammonium chloride (5 M) 50.0 μl of HEPES pH 7 (1 M) 4.0 μl of Magnesium chloride (2.5 M) 536.0 μl of water (100 v/v)
A3	1,6-Hexanediol 35 w/v - tris chloride BCC 0.05 M pH 8.5 Magnesium sulfate 0.075 M - 🔍	pH 8.5	700.0 μl of 1,6-Hexanediol (50 w/v) 75.0 μl of Magnesium sulfate (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 175.0 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 10 v/v - tris chloride BCC 0.05 M pH 7.5 Ammonium acetate 0.05 M - 🔍	pH 7.5	200.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 50.0 μl of Ammonium acetate (1 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 700.0 μl of water (100 v/v)
A5	2-methyl-2,4-pentanediol BCC 10 v/v - HEPES 0.05 M pH 7 Potassium chloride 0.08 M - Magnesium sulfate 0.01 M - 🔍	pH 7	200.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 50.0 μl of HEPES pH 7 (1 M) 10.0 μl of Magnesium sulfate (1 M) 80.0 μl of Potassium chloride (1 M) 660.0 μl of water (100 v/v)
A6	2-methyl-2,4-pentanediol BCC 15 v/v - ADA 0.05 M pH 6.5 Sodium acetate 0.1 M - 🔍	pH 6.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 50.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Sodium acetate (2 M) 600.0 μl of water (100 v/v)
B1	2-methyl-2,4-pentanediol BCC 15 v/v - di-Sodium succinate 0.05 M pH 5.5 Magnesium acetate 0.01 M - 🔍	pH 5.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 10.0 μl of Magnesium acetate (1 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 673.3 μl of water (100 v/v)
B2	2-methyl-2,4-pentanediol BCC 18 v/v - MES 0.02 M pH 5.8 Magnesium chloride 0.01 M - 🔍	pH 5.8	360.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v) 20.0 μl of MES pH 5.8 (1 M) 4.0 μl of Magnesium chloride (2.5 M) 616.0 μl of water (100 v/v)
B3	2-methyl-2,4-pentanediol BCC 23 v/v - MES 0.05 M pH 6 Sodium chloride 0.1 M - 🔍	pH 6	230.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of MES pH 6 (1 M) 100.0 μl of Sodium chloride (1 M) 620.0 μl of water (100 v/v)
B4	2-methyl-2,4-pentanediol BCC 26 v/v - PIPES 0.05 M pH 7 Magnesium chloride 0.065 M - Cobalt (III) Hexamine chloride 0.001 M - 🔍	pH 7	260.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Cobalt (III) Hexamine chloride (0.1 M) 26.0 μl of Magnesium chloride (2.5 M) 50.0 μl of PIPES pH 7 (1 M) 654.0 μl of water (100 v/v)
B5	2-methyl-2,4-pentanediol BCC 27 v/v - MES 0.02 M pH 5.8 Sodium chloride 0.4 M - Calcium chloride 0.12 M - 🔍	pH 5.8	270.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 120.0 μl of Calcium chloride (1 M) 20.0 μl of MES pH 5.8 (1 M) 400.0 μl of Sodium chloride (1 M) 190.0 μl of water (100 v/v)
B6	2-methyl-2,4-pentanediol BCC 35 v/v - BIS-TRIS 0.02 M pH 6 Sodium chloride 0.05 M - Calcium chloride 0.01 M - 🔍	pH 6	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20.0 μl of BIS-TRIS pH 6 (1 M) 10.0 μl of Calcium chloride (1 M) 50.0 μl of Sodium chloride (1 M) 570.0 μl of water (100 v/v)
C1	2-methyl-2,4-pentanediol BCC 50 v/v - tri-Sodium citrate 0.1 M pH 5.6 Magnesium chloride 0.01 M - 🔍	pH 5.6	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 4.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 396.0 μl of water (100 v/v)
C2	2-Propanol 5 v/v - tris chloride BCC 0.05 M pH 7.5 Magnesium chloride 0.01 M - 🔍	pH 7.5	50.0 μl of 2-Propanol (100 v/v) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 896.0 μl of water (100 v/v)
C3	2-Propanol 5 v/v - MES 0.05 M pH 6.5 Calcium acetate 0.1 M - 🔍	pH 6.5	50.0 μl of 2-Propanol (100 v/v) 100.0 μl of Calcium acetate (1 M) 50.0 μl of MES pH 6.5 (1 M) 800.0 μl of water (100 v/v)
C4	2-Propanol 9 v/v - Imidazole 0.05 M pH 7.2 Magnesium acetate 0.015 M - Magnesium chloride 0.015 M - 🔍	pH 7.2	90.0 μl of 2-Propanol (100 v/v) 50.0 μl of Imidazole pH 7.2 (1 M) 15.0 μl of Magnesium acetate (1 M) 6.0 μl of Magnesium chloride (2.5 M) 839.0 μl of water (100 v/v)
C5	2-Propanol 10 v/v - MES 0.05 M pH 6.5 Ammonium acetate 0.08 M - 🔍	pH 6.5	100.0 μl of 2-Propanol (100 v/v) 80.0 μl of Ammonium acetate (1 M) 50.0 μl of MES pH 6.5 (1 M) 770.0 μl of water (100 v/v)
C6	2-Propanol 10 v/v - di-Sodium succinate 0.05 M pH 5.5 Cobalt (II) chloride 0.002 M - 🔍	pH 5.5	100.0 μl of 2-Propanol (100 v/v) 2.0 μl of Cobalt (II) chloride (1 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 881.3 μl of water (100 v/v)
D1	2-Propanol 13 v/v - MOPS 0.05 M pH 7 Potassium chloride 0.2 M - Cobalt (III) Hexamine chloride 0.006 M - 🔍	pH 7	130.0 μl of 2-Propanol (100 v/v) 60.0 μl of Cobalt (III) Hexamine chloride (0.1 M) 50.0 μl of MOPS pH 7 (1 M) 200.0 μl of Potassium chloride (1 M) 560.0 μl of water (100 v/v)
D2	2-Propanol 15 v/v - MES 0.05 M pH 6 Magnesium chloride 0.02 M - 🔍	pH 6	150.0 μl of 2-Propanol (100 v/v) 50.0 μl of MES pH 6 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 792.0 μl of water (100 v/v)
D3	1,4-Dioxane 10 v/v - HEPES 0.05 M pH 7.5 🔍	pH 7.5	200.0 μl of 1,4-Dioxane (50 v/v) 50.0 μl of HEPES pH 7.5 (1 M) 750.0 μl of water (100 v/v)
D4	Ethanol 10 v/v - MES 0.05 M pH 6.5 Magnesium chloride 0.02 M - Cobalt (II) chloride 0.001 M - 🔍	pH 6.5	1.0 μl of Cobalt (II) chloride (1 M) 200.0 μl of Ethanol (50 v/v) 50.0 μl of MES pH 6.5 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 741.0 μl of water (100 v/v)
D5	Ethylene glycol 20 v/v - Polyethylene glycol 3,350 5 w/v - Magnesium chloride 0.02 M - 🔍	NA	400.0 μl of Ethylene glycol (50 v/v) 8.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 3,350 (50 w/v) 492.0 μl of water (100 v/v)
D6	Glycerol 15 v/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.2 M - 🔍	pH 4.6	300.0 μl of Glycerol (50 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)