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Supplemental Information

Screen Description

Nuc-Pro HTS

Nuc-Pro / Nuc-Pro HTS
Created 2009-11-16.

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Nuc-Pro HTS

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Screen Structure

Nuc-Pro HTS

Well Count 96
Number of distinct ingredients 56
Internal diversity (score mean dist) 0.930

Screen Composition

2025-08-16T16:27:05.959167 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Nuc-Pro HTS
Buffer 119
Cryoprotectant 8
Organic (non-volatile) 36
Organic (volatile) 11
Polymer 52
Salt 140

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Nuc-Pro HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 200 20 v/v -
  • HEPES 0.05 M pH 7.5
  • Potassium chloride 0.2 M -
  • Magnesium sulfate 0.025 M -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
25.0 μl of Magnesium sulfate (1 M)
200.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Potassium chloride (5 M)
685.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 200 50 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 400 5 v/v -
  • PIPES 0.05 M pH 7
  • Magnesium chloride 0.03 M -
pH 7
12.0 μl of Magnesium chloride (2.5 M)
50.0 μl of PIPES pH 7 (1 M)
100.0 μl of Polyethylene glycol 400 (50 v/v)
838.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 400 5 v/v -
  • MES 0.02 M pH 5.8
  • Magnesium formate 0.015 M -
  • Cobalt (II) chloride 0.002 M -
pH 5.8
2.0 μl of Cobalt (II) chloride (1 M)
20.0 μl of MES pH 5.8 (1 M)
75.0 μl of Magnesium formate (0.2 M)
100.0 μl of Polyethylene glycol 400 (50 v/v)
803.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 400 10 v/v -
  • HEPES 0.05 M pH 7
  • Potassium chloride 0.1 M -
pH 7
50.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 400 (50 v/v)
20.0 μl of Potassium chloride (5 M)
730.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 400 15 v/v -
  • MES 0.05 M pH 6.5
  • Magnesium acetate 0.08 M -
  • Magnesium chloride 0.015 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium acetate (1 M)
6.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (50 v/v)
564.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 400 15 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Calcium chloride 0.08 M -
  • Sodium chloride 0.02 M -
pH 8
80.0 μl of Calcium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (50 v/v)
20.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 400 20 v/v -
  • BIS-TRIS propane 0.05 M pH 6.8
  • Magnesium chloride 0.06 M -
pH 6.8
50.0 μl of BIS-TRIS propane pH 6.8 (1 M)
24.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 400 (50 v/v)
526.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 400 25 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.13 M -
  • Magnesium chloride 0.06 M -
pH 5.6
24.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 400 (50 v/v)
130.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
246.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 0.2 M -
pH 7.5
40.0 μl of Calcium chloride (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 400 (50 v/v)
260.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.05 M pH 8.5
  • Potassium chloride 0.1 M -
  • Magnesium chloride 0.01 M -
pH 8.5
4.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of Potassium chloride (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
246.0 μl of water (100 v/v)
A12
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • HEPES 0.05 M pH 7
  • Magnesium chloride 0.01 M -
pH 7
50.0 μl of HEPES pH 7 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
446.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 1,000 20 w/v -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.2 M -
  • Sodium chloride 0.1 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
370.0 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.2 M -
pH 4.6
200.0 μl of Ammonium sulfate (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 10 w/v -
  • MES 0.1 M pH 6.5
  • Calcium chloride 0.1 M -
  • Glycerol 13 v/v -
pH 6.5
100.0 μl of Calcium chloride (1 M)
260.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
340.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 25 w/v -
  • MES 0.05 M pH 6
  • Sodium formate 0.2 M -
  • Glycerol 10 v/v -
pH 6
200.0 μl of Glycerol (50 v/v)
50.0 μl of MES pH 6 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium formate (5 M)
210.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 30 w/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Sodium chloride 0.1 M -
pH 5.5
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium chloride (1 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
283.3 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 35 w/v -
  • tris chloride BCC 0.05 M pH 7.5
  • Potassium chloride 0.05 M -
pH 7.5
700.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (1 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
200.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 4,000 5 w/v -
  • HEPES 0.05 M pH 7
  • Ammonium sulfate 0.2 M -
  • Magnesium acetate 0.02 M -
pH 7
200.0 μl of Ammonium sulfate (1 M)
50.0 μl of HEPES pH 7 (1 M)
20.0 μl of Magnesium acetate (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
630.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 4,000 5 w/v -
  • MES 0.05 M pH 6
  • Magnesium sulfate 0.005 M -
pH 6
50.0 μl of MES pH 6 (1 M)
5.0 μl of Magnesium sulfate (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
845.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 4,000 10 w/v -
  • MES 0.05 M pH 6.5
  • Ammonium acetate 0.2 M -
  • Calcium chloride 0.01 M -
pH 6.5
200.0 μl of Ammonium acetate (1 M)
10.0 μl of Calcium chloride (1 M)
50.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
540.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 4,000 10 w/v -
  • Imidazole 0.05 M pH 7.2
  • Zinc sulfate 0.02 M -
pH 7.2
50.0 μl of Imidazole pH 7.2 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Zinc sulfate (1 M)
730.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 4,000 15 w/v -
  • tris chloride BCC 0.05 M pH 7.5
  • Potassium chloride 0.15 M -
  • Magnesium chloride 0.02 M -
pH 7.5
8.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
150.0 μl of Potassium chloride (1 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
492.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 4,000 15 w/v -
  • tri-Sodium citrate 0.05 M pH 5
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 0.02 M -
pH 5
20.0 μl of Ammonium sulfate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 5 (1 M)
530.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 4,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Potassium chloride 0.1 M -
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
375.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 4,000 20 w/v -
  • MOPS 0.05 M pH 7
  • Sodium chloride 0.1 M -
pH 7
50.0 μl of MOPS pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
450.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 4,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 4,000 24 w/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Magnesium chloride 0.06 M -
  • Sodium chloride 0.3 M -
pH 5.5
24.0 μl of Magnesium chloride (2.5 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of Sodium chloride (1 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
179.3 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 25 w/v -
  • tri-Sodium citrate 0.05 M pH 5
  • Ammonium sulfate 0.05 M -
pH 5
50.0 μl of Ammonium sulfate (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tri-Sodium citrate pH 5 (1 M)
400.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 30 w/v -
  • MES 0.05 M pH 6.5
  • Magnesium acetate 0.08 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
270.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.05 M pH 8.5
  • Ammonium chloride 0.15 M -
  • Calcium chloride 0.01 M -
pH 8.5
30.0 μl of Ammonium chloride (5 M)
10.0 μl of Calcium chloride (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
310.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 4,000 32 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 5 v/v -
pH 8.5
50.0 μl of Glycerol (100 v/v)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
210.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 4,000 36 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
720.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 5 (1 M)
230.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 6,000 5 w/v -
  • BIS-TRIS 0.02 M pH 6
  • di-Ammonium hydrogen citrate 0.06 M -
  • Magnesium chloride 0.02 M -
pH 6
20.0 μl of BIS-TRIS pH 6 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
60.0 μl of di-Ammonium hydrogen citrate (1 M)
812.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 6,000 10 w/v -
  • tri-Sodium citrate 0.05 M pH 4
  • Sodium chloride 0.1 M -
pH 4
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 4 (1 M)
650.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.05 M pH 7
  • Ammonium acetate 0.2 M -
  • Magnesium acetate 0.15 M -
pH 7
200.0 μl of Ammonium acetate (1 M)
50.0 μl of HEPES pH 7 (1 M)
150.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 6,000 15 w/v -
  • tris chloride BCC 0.01 M pH 7.5
pH 7.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
690.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 6,000 18 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
360.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium acetate pH 5 (1 M)
590.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 6,000 20 w/v -
  • BIS-TRIS propane 0.05 M pH 7
  • 2-methyl-2,4-pentanediol BCC 7 v/v -
  • 2-Methyl-2-propanol 5 v/v -
pH 7
100.0 μl of 2-Methyl-2-propanol (50 v/v)
140.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
50.0 μl of BIS-TRIS propane pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
310.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 6,000 20 w/v -
  • di-Potassium L-Malate 0.05 M pH 5
  • Calcium chloride 0.03 M -
pH 5
30.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of di-Potassium L-Malate pH 5 (1 M)
520.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 8,000 5 w/v -
  • HEPES 0.05 M pH 7.5
  • Magnesium chloride 0.02 M -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
842.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Glycerol 10 v/v -
  • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
pH 8
200.0 μl of Glycerol (50 v/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
499.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 8,000 10 w/v -
  • MES 0.05 M pH 6.5
  • Potassium chloride 0.2 M -
  • Magnesium acetate 0.1 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Potassium chloride (1 M)
450.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 8,000 15 w/v -
  • BIS-TRIS propane 0.05 M pH 6.8
  • Ammonium sulfate 0.1 M -
  • Glycerol 10 v/v -
pH 6.8
100.0 μl of Ammonium sulfate (1 M)
50.0 μl of BIS-TRIS propane pH 6.8 (1 M)
200.0 μl of Glycerol (50 v/v)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
350.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 8,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 8,000 18 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium formate 0.2 M -
pH 8
200.0 μl of Magnesium formate (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.01 M pH 7.5
  • Calcium chloride 0.01 M -
pH 7.5
10.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
580.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 20,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Manganese (II) chloride 0.08 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Manganese (II) chloride (1 M)
300.0 μl of Polyethylene glycol 20,000 (50 w/v)
520.0 μl of water (100 v/v)
E1
  • Ammonium sulfate 1.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 w/v -
pH 7.5
240.0 μl of Ammonium sulfate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 400 (50 w/v)
620.0 μl of water (100 v/v)
E2
  • Calcium chloride 0.02 M -
  • Glycine 0.1 M pH 8
pH 8
20.0 μl of Calcium chloride (1 M)
100.0 μl of Glycine pH 8 (1 M)
880.0 μl of water (100 v/v)
E3
  • Lithium chloride 2 M -
  • MES 0.05 M pH 6
  • Calcium acetate 0.2 M -
  • Cobalt (II) chloride 0.001 M -
pH 6
200.0 μl of Calcium acetate (1 M)
1.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Lithium chloride (5 M)
50.0 μl of MES pH 6 (1 M)
349.0 μl of water (100 v/v)
E4
  • Lithium sulfate 0.6 M -
  • MES 0.05 M pH 6
  • Magnesium chloride 0.01 M -
pH 6
600.0 μl of Lithium sulfate (1 M)
50.0 μl of MES pH 6 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
346.0 μl of water (100 v/v)
E5
  • Lithium sulfate 1 M -
  • Polyethylene glycol 400 8 w/v -
  • HEPES 0.05 M pH 7.5
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Lithium sulfate (2.5 M)
160.0 μl of Polyethylene glycol 400 (50 w/v)
390.0 μl of water (100 v/v)
E6
  • Lithium sulfate 1 M -
  • tri-Sodium citrate 0.05 M -
  • 2-Propanol 3 w/v -
NA
60.0 μl of 2-Propanol (50 w/v)
400.0 μl of Lithium sulfate (2.5 M)
25.0 μl of tri-Sodium citrate (2 M)
515.0 μl of water (100 v/v)
E7
  • Lithium sulfate 1.2 M -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.03 M -
pH 6.5
480.0 μl of Lithium sulfate (2.5 M)
50.0 μl of MES pH 6.5 (1 M)
12.0 μl of Magnesium chloride (2.5 M)
458.0 μl of water (100 v/v)
E8
  • Lithium sulfate 1.2 M -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.05 M -
  • Cobalt (II) chloride 0.002 M -
pH 6.5
2.0 μl of Cobalt (II) chloride (1 M)
480.0 μl of Lithium sulfate (2.5 M)
50.0 μl of MES pH 6.5 (1 M)
20.0 μl of Magnesium chloride (2.5 M)
448.0 μl of water (100 v/v)
E9
  • Lithium sulfate 1.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Glycerol 5 w/v -
pH 8.5
100.0 μl of Glycerol (50 w/v)
600.0 μl of Lithium sulfate (2.5 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
E10
  • Lithium sulfate 1.6 M -
  • Polyethylene glycol 1,000 2 w/v -
  • HEPES 0.05 M pH 7.5
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
640.0 μl of Lithium sulfate (2.5 M)
40.0 μl of Polyethylene glycol 1,000 (50 w/v)
270.0 μl of water (100 v/v)
E11
  • Lithium sulfate 1.7 M -
  • HEPES 0.05 M pH 7
  • Magnesium sulfate 0.05 M -
pH 7
50.0 μl of HEPES pH 7 (1 M)
680.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium sulfate (1 M)
220.0 μl of water (100 v/v)
E12
  • Lithium sulfate 1.7 M -
  • Glycerol 10 w/v -
NA
200.0 μl of Glycerol (50 w/v)
680.0 μl of Lithium sulfate (2.5 M)
120.0 μl of water (100 v/v)
F1
  • Lithium sulfate 2 M -
  • 2-methyl-2,4-pentanediol BCC 3 w/v -
NA
33.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
800.0 μl of Lithium sulfate (2.5 M)
166.7 μl of water (100 v/v)
F2
  • Magnesium chloride 0.005 M -
  • MES 0.05 M pH 6.5
  • Cobalt (II) chloride 0.0025 M -
pH 6.5
2.5 μl of Cobalt (II) chloride (1 M)
50.0 μl of MES pH 6.5 (1 M)
2.0 μl of Magnesium chloride (2.5 M)
945.5 μl of water (100 v/v)
F3
  • Magnesium chloride 0.04 M -
  • MES 0.05 M pH 6
pH 6
50.0 μl of MES pH 6 (1 M)
16.0 μl of Magnesium chloride (2.5 M)
934.0 μl of water (100 v/v)
F4
  • Magnesium chloride 0.08 M -
  • HEPES 0.05 M pH 7.5
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
32.0 μl of Magnesium chloride (2.5 M)
918.0 μl of water (100 v/v)
F5
  • Sodium chloride 0.2 M -
  • BIS-TRIS propane 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS propane pH 7 (1 M)
200.0 μl of Sodium chloride (1 M)
750.0 μl of water (100 v/v)
F6
  • Sodium chloride 0.4 M -
  • BIS-TRIS propane 0.05 M pH 6.8
pH 6.8
50.0 μl of BIS-TRIS propane pH 6.8 (1 M)
400.0 μl of Sodium chloride (1 M)
550.0 μl of water (100 v/v)
F7
  • Sodium chloride 0.6 M -
  • MES 0.05 M pH 6
  • Ammonium acetate 0.1 M -
  • Magnesium sulfate 0.005 M -
pH 6
100.0 μl of Ammonium acetate (1 M)
50.0 μl of MES pH 6 (1 M)
5.0 μl of Magnesium sulfate (1 M)
120.0 μl of Sodium chloride (5 M)
725.0 μl of water (100 v/v)
F8
  • Sodium chloride 2.5 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Sodium chloride (5 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
370.0 μl of water (100 v/v)
F9
  • Sodium formate 1.8 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
360.0 μl of Sodium formate (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
540.0 μl of water (100 v/v)
F10
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium formate (5 M)
500.0 μl of water (100 v/v)
F11
  • di-Sodium hydrogen phosphate 0.1 M -
  • Sodium chloride 0.08 M -
NA
80.0 μl of Sodium chloride (1 M)
20.0 μl of di-Sodium hydrogen phosphate (5 M)
900.0 μl of water (100 v/v)
F12
  • di-Sodium tartrate 1 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Magnesium chloride 0.03 M -
pH 7.5
12.0 μl of Magnesium chloride (2.5 M)
500.0 μl of di-Sodium tartrate (2 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
438.0 μl of water (100 v/v)
G1
  • 1,6-Hexanediol 10 w/v -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.02 M -
pH 6.5
200.0 μl of 1,6-Hexanediol (50 w/v)
50.0 μl of MES pH 6.5 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
742.0 μl of water (100 v/v)
G2
  • 1,6-Hexanediol 20 w/v -
  • HEPES 0.05 M pH 7
  • Ammonium chloride 0.05 M -
  • Magnesium chloride 0.01 M -
pH 7
400.0 μl of 1,6-Hexanediol (50 w/v)
10.0 μl of Ammonium chloride (5 M)
50.0 μl of HEPES pH 7 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
536.0 μl of water (100 v/v)
G3
  • 1,6-Hexanediol 35 w/v -
  • tris chloride BCC 0.05 M pH 8.5
  • Magnesium sulfate 0.075 M -
pH 8.5
700.0 μl of 1,6-Hexanediol (50 w/v)
75.0 μl of Magnesium sulfate (1 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
175.0 μl of water (100 v/v)
G4
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • tris chloride BCC 0.05 M pH 7.5
  • Ammonium acetate 0.05 M -
pH 7.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
50.0 μl of Ammonium acetate (1 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
700.0 μl of water (100 v/v)
G5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • HEPES 0.05 M pH 7
  • Potassium chloride 0.08 M -
  • Magnesium sulfate 0.01 M -
pH 7
200.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
50.0 μl of HEPES pH 7 (1 M)
10.0 μl of Magnesium sulfate (1 M)
80.0 μl of Potassium chloride (1 M)
660.0 μl of water (100 v/v)
G6
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • ADA 0.05 M pH 6.5
  • Sodium acetate 0.1 M -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
50.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Sodium acetate (2 M)
600.0 μl of water (100 v/v)
G7
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Magnesium acetate 0.01 M -
pH 5.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
10.0 μl of Magnesium acetate (1 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
673.3 μl of water (100 v/v)
G8
  • 2-methyl-2,4-pentanediol BCC 18 v/v -
  • MES 0.02 M pH 5.8
  • Magnesium chloride 0.01 M -
pH 5.8
360.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
20.0 μl of MES pH 5.8 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
616.0 μl of water (100 v/v)
G9
  • 2-methyl-2,4-pentanediol BCC 23 v/v -
  • MES 0.05 M pH 6
  • Sodium chloride 0.1 M -
pH 6
230.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of MES pH 6 (1 M)
100.0 μl of Sodium chloride (1 M)
620.0 μl of water (100 v/v)
G10
  • 2-methyl-2,4-pentanediol BCC 26 v/v -
  • PIPES 0.05 M pH 7
  • Magnesium chloride 0.065 M -
  • Cobalt (III) Hexamine chloride 0.001 M -
pH 7
260.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
26.0 μl of Magnesium chloride (2.5 M)
50.0 μl of PIPES pH 7 (1 M)
654.0 μl of water (100 v/v)
G11
  • 2-methyl-2,4-pentanediol BCC 27 v/v -
  • MES 0.02 M pH 5.8
  • Sodium chloride 0.4 M -
  • Calcium chloride 0.12 M -
pH 5.8
270.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
120.0 μl of Calcium chloride (1 M)
20.0 μl of MES pH 5.8 (1 M)
400.0 μl of Sodium chloride (1 M)
190.0 μl of water (100 v/v)
G12
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • BIS-TRIS 0.02 M pH 6
  • Sodium chloride 0.05 M -
  • Calcium chloride 0.01 M -
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of BIS-TRIS pH 6 (1 M)
10.0 μl of Calcium chloride (1 M)
50.0 μl of Sodium chloride (1 M)
570.0 μl of water (100 v/v)
H1
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Magnesium chloride 0.01 M -
pH 5.6
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
396.0 μl of water (100 v/v)
H2
  • 2-Propanol 5 v/v -
  • tris chloride BCC 0.05 M pH 7.5
  • Magnesium chloride 0.01 M -
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
4.0 μl of Magnesium chloride (2.5 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
896.0 μl of water (100 v/v)
H3
  • 2-Propanol 5 v/v -
  • MES 0.05 M pH 6.5
  • Calcium acetate 0.1 M -
pH 6.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium acetate (1 M)
50.0 μl of MES pH 6.5 (1 M)
800.0 μl of water (100 v/v)
H4
  • 2-Propanol 9 v/v -
  • Imidazole 0.05 M pH 7.2
  • Magnesium acetate 0.015 M -
  • Magnesium chloride 0.015 M -
pH 7.2
90.0 μl of 2-Propanol (100 v/v)
50.0 μl of Imidazole pH 7.2 (1 M)
15.0 μl of Magnesium acetate (1 M)
6.0 μl of Magnesium chloride (2.5 M)
839.0 μl of water (100 v/v)
H5
  • 2-Propanol 10 v/v -
  • MES 0.05 M pH 6.5
  • Ammonium acetate 0.08 M -
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
80.0 μl of Ammonium acetate (1 M)
50.0 μl of MES pH 6.5 (1 M)
770.0 μl of water (100 v/v)
H6
  • 2-Propanol 10 v/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Cobalt (II) chloride 0.002 M -
pH 5.5
100.0 μl of 2-Propanol (100 v/v)
2.0 μl of Cobalt (II) chloride (1 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
881.3 μl of water (100 v/v)
H7
  • 2-Propanol 13 v/v -
  • MOPS 0.05 M pH 7
  • Potassium chloride 0.2 M -
  • Cobalt (III) Hexamine chloride 0.006 M -
pH 7
130.0 μl of 2-Propanol (100 v/v)
60.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
50.0 μl of MOPS pH 7 (1 M)
200.0 μl of Potassium chloride (1 M)
560.0 μl of water (100 v/v)
H8
  • 2-Propanol 15 v/v -
  • MES 0.05 M pH 6
  • Magnesium chloride 0.02 M -
pH 6
150.0 μl of 2-Propanol (100 v/v)
50.0 μl of MES pH 6 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
792.0 μl of water (100 v/v)
H9
  • 1,4-Dioxane 10 v/v -
  • HEPES 0.05 M pH 7.5
pH 7.5
200.0 μl of 1,4-Dioxane (50 v/v)
50.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of water (100 v/v)
H10
  • Ethanol 10 v/v -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.02 M -
  • Cobalt (II) chloride 0.001 M -
pH 6.5
1.0 μl of Cobalt (II) chloride (1 M)
200.0 μl of Ethanol (50 v/v)
50.0 μl of MES pH 6.5 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
741.0 μl of water (100 v/v)
H11
  • Ethylene glycol 20 v/v -
  • Polyethylene glycol 3,350 5 w/v -
  • Magnesium chloride 0.02 M -
NA
400.0 μl of Ethylene glycol (50 v/v)
8.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
492.0 μl of water (100 v/v)
H12
  • Glycerol 15 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 0.2 M -
pH 4.6
300.0 μl of Glycerol (50 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
200.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)