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Supplemental Information

Screen Description

Nuc-Pro HTS

Nuc-Pro / Nuc-Pro HTS
Created 2009-11-16.

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Nuc-Pro HTS

    Screen Structure

    Nuc-Pro HTS

    Well Count 96
    Number of distinct ingredients 56
    Internal diversity (score mean dist) 0.930

    Screen Composition

    2025-06-14T13:23:29.097523 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Nuc-Pro HTS
    Buffer 119
    Cryoprotectant 8
    Organic (non-volatile) 36
    Organic (volatile) 11
    Polymer 52
    Salt 140

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Nuc-Pro HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 200 20 v/v -
    • HEPES 0.05 M pH 7.5
    • Potassium chloride 0.2 M -
    • Magnesium sulfate 0.025 M -
    pH 7.5
    25 μl of Magnesium sulfate (1 M)
    200 μl of Polyethylene glycol 200 (100 v/v)
    40 μl of Potassium chloride (5 M)
    50 μl of HEPES pH 7.5 (1 M)
    685 μl of water (100 v/v)
    A2
    • Polyethylene glycol 200 50 v/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    500 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of water (100 v/v)
    A3
    • Polyethylene glycol 400 5 v/v -
    • PIPES 0.05 M pH 7
    • Magnesium chloride 0.03 M -
    pH 7
    12 μl of Magnesium chloride (2.5 M)
    50 μl of PIPES pH 7 (1 M)
    100 μl of Polyethylene glycol 400 (50 v/v)
    838 μl of water (100 v/v)
    A4
    • Polyethylene glycol 400 5 v/v -
    • MES 0.02 M pH 5.8
    • Magnesium formate 0.015 M -
    • Cobalt (II) chloride 0.002 M -
    pH 5.8
    75 μl of Magnesium formate (0.2 M)
    2 μl of Cobalt (II) chloride (1 M)
    20 μl of MES pH 5.8 (1 M)
    100 μl of Polyethylene glycol 400 (50 v/v)
    803 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 10 v/v -
    • HEPES 0.05 M pH 7
    • Potassium chloride 0.1 M -
    pH 7
    20 μl of Potassium chloride (5 M)
    200 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of HEPES pH 7 (1 M)
    730 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 15 v/v -
    • MES 0.05 M pH 6.5
    • Magnesium acetate 0.08 M -
    • Magnesium chloride 0.015 M -
    pH 6.5
    80 μl of Magnesium acetate (1 M)
    6 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of MES pH 6.5 (1 M)
    564 μl of water (100 v/v)
    A7
    • Polyethylene glycol 400 15 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Calcium chloride 0.08 M -
    • Sodium chloride 0.02 M -
    pH 8
    20 μl of Sodium chloride (1 M)
    80 μl of Calcium chloride (1 M)
    300 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    A8
    • Polyethylene glycol 400 20 v/v -
    • BIS-TRIS propane 0.05 M pH 6.8
    • Magnesium chloride 0.06 M -
    pH 6.8
    24 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of BIS-TRIS propane pH 6.8 (1 M)
    526 μl of water (100 v/v)
    A9
    • Polyethylene glycol 400 25 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.13 M -
    • Magnesium chloride 0.06 M -
    pH 5.6
    130 μl of Sodium chloride (1 M)
    24 μl of Magnesium chloride (2.5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    246 μl of water (100 v/v)
    A10
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    pH 7.5
    40 μl of Calcium chloride (5 M)
    600 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    260 μl of water (100 v/v)
    A11
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.05 M pH 8.5
    • Potassium chloride 0.1 M -
    • Magnesium chloride 0.01 M -
    pH 8.5
    4 μl of Magnesium chloride (2.5 M)
    100 μl of Potassium chloride (1 M)
    600 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    246 μl of water (100 v/v)
    A12
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    • HEPES 0.05 M pH 7
    • Magnesium chloride 0.01 M -
    pH 7
    4 μl of Magnesium chloride (2.5 M)
    50 μl of HEPES pH 7 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    446 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,000 20 w/v -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.2 M -
    • Sodium chloride 0.1 M -
    pH 6.5
    100 μl of Sodium chloride (1 M)
    80 μl of Magnesium chloride (2.5 M)
    50 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    370 μl of water (100 v/v)
    B2
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    pH 4.6
    200 μl of Ammonium sulfate (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,350 10 w/v -
    • MES 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    • Glycerol 13 v/v -
    pH 6.5
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Calcium chloride (1 M)
    260 μl of Glycerol (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    340 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 25 w/v -
    • MES 0.05 M pH 6
    • Sodium formate 0.2 M -
    • Glycerol 10 v/v -
    pH 6
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Glycerol (50 v/v)
    40 μl of Sodium formate (5 M)
    50 μl of MES pH 6 (1 M)
    210 μl of water (100 v/v)
    B5
    • Polyethylene glycol 3,350 30 w/v -
    • di-Sodium succinate 0.05 M pH 5.5
    • Sodium chloride 0.1 M -
    pH 5.5
    100 μl of Sodium chloride (1 M)
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    16 μl of di-Sodium succinate pH 5.5 (3 M)
    283 μl of water (100 v/v)
    B6
    • Polyethylene glycol 3,350 35 w/v -
    • tris chloride BCC 0.05 M pH 7.5
    • Potassium chloride 0.05 M -
    pH 7.5
    700 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Potassium chloride (1 M)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    200 μl of water (100 v/v)
    B7
    • Polyethylene glycol 4,000 5 w/v -
    • HEPES 0.05 M pH 7
    • Ammonium sulfate 0.2 M -
    • Magnesium acetate 0.02 M -
    pH 7
    20 μl of Magnesium acetate (1 M)
    200 μl of Ammonium sulfate (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    630 μl of water (100 v/v)
    B8
    • Polyethylene glycol 4,000 5 w/v -
    • MES 0.05 M pH 6
    • Magnesium sulfate 0.005 M -
    pH 6
    5 μl of Magnesium sulfate (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of MES pH 6 (1 M)
    845 μl of water (100 v/v)
    B9
    • Polyethylene glycol 4,000 10 w/v -
    • MES 0.05 M pH 6.5
    • Ammonium acetate 0.2 M -
    • Calcium chloride 0.01 M -
    pH 6.5
    10 μl of Calcium chloride (1 M)
    200 μl of Ammonium acetate (1 M)
    50 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    540 μl of water (100 v/v)
    B10
    • Polyethylene glycol 4,000 10 w/v -
    • Imidazole 0.05 M pH 7.2
    • Zinc sulfate 0.02 M -
    pH 7.2
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Imidazole pH 7.2 (1 M)
    20 μl of Zinc sulfate (1 M)
    730 μl of water (100 v/v)
    B11
    • Polyethylene glycol 4,000 15 w/v -
    • tris chloride BCC 0.05 M pH 7.5
    • Potassium chloride 0.15 M -
    • Magnesium chloride 0.02 M -
    pH 7.5
    8 μl of Magnesium chloride (2.5 M)
    150 μl of Potassium chloride (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    492 μl of water (100 v/v)
    B12
    • Polyethylene glycol 4,000 15 w/v -
    • tri-Sodium citrate 0.05 M pH 5
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 0.02 M -
    pH 5
    100 μl of Sodium chloride (1 M)
    50 μl of tri-Sodium citrate pH 5 (1 M)
    20 μl of Ammonium sulfate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    530 μl of water (100 v/v)
    C1
    • Polyethylene glycol 4,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Potassium chloride 0.1 M -
    • 2-Propanol 10 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of 2-Propanol (100 v/v)
    25 μl of Potassium chloride (4 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    375 μl of water (100 v/v)
    C2
    • Polyethylene glycol 4,000 20 w/v -
    • MOPS 0.05 M pH 7
    • Sodium chloride 0.1 M -
    pH 7
    100 μl of Sodium chloride (1 M)
    50 μl of MOPS pH 7 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    450 μl of water (100 v/v)
    C3
    • Polyethylene glycol 4,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    C4
    • Polyethylene glycol 4,000 24 w/v -
    • di-Sodium succinate 0.05 M pH 5.5
    • Magnesium chloride 0.06 M -
    • Sodium chloride 0.3 M -
    pH 5.5
    300 μl of Sodium chloride (1 M)
    24 μl of Magnesium chloride (2.5 M)
    16 μl of di-Sodium succinate pH 5.5 (3 M)
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    179 μl of water (100 v/v)
    C5
    • Polyethylene glycol 4,000 25 w/v -
    • tri-Sodium citrate 0.05 M pH 5
    • Ammonium sulfate 0.05 M -
    pH 5
    50 μl of tri-Sodium citrate pH 5 (1 M)
    50 μl of Ammonium sulfate (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Polyethylene glycol 4,000 30 w/v -
    • MES 0.05 M pH 6.5
    • Magnesium acetate 0.08 M -
    pH 6.5
    80 μl of Magnesium acetate (1 M)
    50 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    270 μl of water (100 v/v)
    C7
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.05 M pH 8.5
    • Ammonium chloride 0.15 M -
    • Calcium chloride 0.01 M -
    pH 8.5
    30 μl of Ammonium chloride (5 M)
    10 μl of Calcium chloride (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    310 μl of water (100 v/v)
    C8
    • Polyethylene glycol 4,000 32 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 5 v/v -
    pH 8.5
    50 μl of Glycerol (100 v/v)
    640 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    210 μl of water (100 v/v)
    C9
    • Polyethylene glycol 4,000 36 w/v -
    • Sodium acetate 0.05 M pH 5
    pH 5
    50 μl of Sodium acetate pH 5 (1 M)
    720 μl of Polyethylene glycol 4,000 (50 w/v)
    230 μl of water (100 v/v)
    C10
    • Polyethylene glycol 6,000 5 w/v -
    • BIS-TRIS 0.02 M pH 6
    • di-Ammonium hydrogen citrate 0.06 M -
    • Magnesium chloride 0.02 M -
    pH 6
    8 μl of Magnesium chloride (2.5 M)
    20 μl of BIS-TRIS pH 6 (1 M)
    60 μl of di-Ammonium hydrogen citrate (1 M)
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    812 μl of water (100 v/v)
    C11
    • Polyethylene glycol 6,000 10 w/v -
    • tri-Sodium citrate 0.05 M pH 4
    • Sodium chloride 0.1 M -
    pH 4
    100 μl of Sodium chloride (1 M)
    50 μl of tri-Sodium citrate pH 4 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    650 μl of water (100 v/v)
    C12
    • Polyethylene glycol 6,000 10 w/v -
    • HEPES 0.05 M pH 7
    • Ammonium acetate 0.2 M -
    • Magnesium acetate 0.15 M -
    pH 7
    150 μl of Magnesium acetate (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    400 μl of water (100 v/v)
    D1
    • Polyethylene glycol 6,000 15 w/v -
    • tris chloride BCC 0.01 M pH 7.5
    pH 7.5
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    10 μl of tris chloride BCC pH 7.5 (1 M)
    690 μl of water (100 v/v)
    D2
    • Polyethylene glycol 6,000 18 w/v -
    • Sodium acetate 0.05 M pH 5
    pH 5
    50 μl of Sodium acetate pH 5 (1 M)
    360 μl of Polyethylene glycol 6,000 (50 w/v)
    590 μl of water (100 v/v)
    D3
    • Polyethylene glycol 6,000 20 w/v -
    • BIS-TRIS propane 0.05 M pH 7
    • 2-methyl-2,4-pentanediol BCC 7 v/v -
    • 2-Methyl-2-propanol 5 v/v -
    pH 7
    50 μl of BIS-TRIS propane pH 7 (1 M)
    100 μl of 2-Methyl-2-propanol (50 v/v)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    140 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    310 μl of water (100 v/v)
    D4
    • Polyethylene glycol 6,000 20 w/v -
    • di-Potassium L-Malate 0.05 M pH 5
    • Calcium chloride 0.03 M -
    pH 5
    30 μl of Calcium chloride (1 M)
    50 μl of di-Potassium L-Malate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    520 μl of water (100 v/v)
    D5
    • Polyethylene glycol 8,000 5 w/v -
    • HEPES 0.05 M pH 7.5
    • Magnesium chloride 0.02 M -
    pH 7.5
    8 μl of Magnesium chloride (2.5 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of HEPES pH 7.5 (1 M)
    842 μl of water (100 v/v)
    D6
    • Polyethylene glycol 8,000 10 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Glycerol 10 v/v -
    • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
    pH 8
    200 μl of Glycerol (50 v/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    1 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
    499 μl of water (100 v/v)
    D7
    • Polyethylene glycol 8,000 10 w/v -
    • MES 0.05 M pH 6.5
    • Potassium chloride 0.2 M -
    • Magnesium acetate 0.1 M -
    pH 6.5
    100 μl of Magnesium acetate (1 M)
    200 μl of Potassium chloride (1 M)
    50 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    450 μl of water (100 v/v)
    D8
    • Polyethylene glycol 8,000 15 w/v -
    • BIS-TRIS propane 0.05 M pH 6.8
    • Ammonium sulfate 0.1 M -
    • Glycerol 10 v/v -
    pH 6.8
    100 μl of Ammonium sulfate (1 M)
    200 μl of Glycerol (50 v/v)
    50 μl of BIS-TRIS propane pH 6.8 (1 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    350 μl of water (100 v/v)
    D9
    • Polyethylene glycol 8,000 15 w/v -
    • MES 0.1 M pH 6.5
    • Calcium acetate 0.2 M -
    pH 6.5
    200 μl of Calcium acetate (1 M)
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of water (100 v/v)
    D10
    • Polyethylene glycol 8,000 18 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Magnesium formate 0.2 M -
    pH 8
    200 μl of Magnesium formate (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    340 μl of water (100 v/v)
    D11
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.01 M pH 7.5
    • Calcium chloride 0.01 M -
    pH 7.5
    10 μl of Calcium chloride (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    10 μl of tris chloride BCC pH 7.5 (1 M)
    580 μl of water (100 v/v)
    D12
    • Polyethylene glycol 20,000 15 w/v -
    • MES 0.1 M pH 6.5
    • Manganese (II) chloride 0.08 M -
    pH 6.5
    80 μl of Manganese (II) chloride (1 M)
    300 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    520 μl of water (100 v/v)
    E1
    • Ammonium sulfate 1.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 w/v -
    pH 7.5
    40 μl of Polyethylene glycol 400 (50 w/v)
    240 μl of Ammonium sulfate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    E2
    • Calcium chloride 0.02 M -
    • Glycine 0.1 M pH 8
    pH 8
    20 μl of Calcium chloride (1 M)
    100 μl of Glycine pH 8 (1 M)
    880 μl of water (100 v/v)
    E3
    • Lithium chloride 2 M -
    • MES 0.05 M pH 6
    • Calcium acetate 0.2 M -
    • Cobalt (II) chloride 0.001 M -
    pH 6
    200 μl of Calcium acetate (1 M)
    1 μl of Cobalt (II) chloride (1 M)
    400 μl of Lithium chloride (5 M)
    50 μl of MES pH 6 (1 M)
    349 μl of water (100 v/v)
    E4
    • Lithium sulfate 0.6 M -
    • MES 0.05 M pH 6
    • Magnesium chloride 0.01 M -
    pH 6
    4 μl of Magnesium chloride (2.5 M)
    600 μl of Lithium sulfate (1 M)
    50 μl of MES pH 6 (1 M)
    346 μl of water (100 v/v)
    E5
    • Lithium sulfate 1 M -
    • Polyethylene glycol 400 8 w/v -
    • HEPES 0.05 M pH 7.5
    pH 7.5
    160 μl of Polyethylene glycol 400 (50 w/v)
    400 μl of Lithium sulfate (2.5 M)
    50 μl of HEPES pH 7.5 (1 M)
    390 μl of water (100 v/v)
    E6
    • Lithium sulfate 1 M -
    • tri-Sodium citrate 0.05 M -
    • 2-Propanol 3 w/v -
    NA
    400 μl of Lithium sulfate (2.5 M)
    25 μl of tri-Sodium citrate (2 M)
    60 μl of 2-Propanol (50 w/v)
    515 μl of water (100 v/v)
    E7
    • Lithium sulfate 1.2 M -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.03 M -
    pH 6.5
    12 μl of Magnesium chloride (2.5 M)
    480 μl of Lithium sulfate (2.5 M)
    50 μl of MES pH 6.5 (1 M)
    458 μl of water (100 v/v)
    E8
    • Lithium sulfate 1.2 M -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.05 M -
    • Cobalt (II) chloride 0.002 M -
    pH 6.5
    20 μl of Magnesium chloride (2.5 M)
    2 μl of Cobalt (II) chloride (1 M)
    480 μl of Lithium sulfate (2.5 M)
    50 μl of MES pH 6.5 (1 M)
    448 μl of water (100 v/v)
    E9
    • Lithium sulfate 1.5 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Glycerol 5 w/v -
    pH 8.5
    600 μl of Lithium sulfate (2.5 M)
    100 μl of Glycerol (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of water (100 v/v)
    E10
    • Lithium sulfate 1.6 M -
    • Polyethylene glycol 1,000 2 w/v -
    • HEPES 0.05 M pH 7.5
    pH 7.5
    640 μl of Lithium sulfate (2.5 M)
    40 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of HEPES pH 7.5 (1 M)
    270 μl of water (100 v/v)
    E11
    • Lithium sulfate 1.7 M -
    • HEPES 0.05 M pH 7
    • Magnesium sulfate 0.05 M -
    pH 7
    50 μl of Magnesium sulfate (1 M)
    680 μl of Lithium sulfate (2.5 M)
    50 μl of HEPES pH 7 (1 M)
    220 μl of water (100 v/v)
    E12
    • Lithium sulfate 1.7 M -
    • Glycerol 10 w/v -
    NA
    680 μl of Lithium sulfate (2.5 M)
    200 μl of Glycerol (50 w/v)
    120 μl of water (100 v/v)
    F1
    • Lithium sulfate 2 M -
    • 2-methyl-2,4-pentanediol BCC 3 w/v -
    NA
    800 μl of Lithium sulfate (2.5 M)
    33 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    166 μl of water (100 v/v)
    F2
    • Magnesium chloride 0.005 M -
    • MES 0.05 M pH 6.5
    • Cobalt (II) chloride 0.0025 M -
    pH 6.5
    2 μl of Magnesium chloride (2.5 M)
    2 μl of Cobalt (II) chloride (1 M)
    50 μl of MES pH 6.5 (1 M)
    945 μl of water (100 v/v)
    F3
    • Magnesium chloride 0.04 M -
    • MES 0.05 M pH 6
    pH 6
    16 μl of Magnesium chloride (2.5 M)
    50 μl of MES pH 6 (1 M)
    934 μl of water (100 v/v)
    F4
    • Magnesium chloride 0.08 M -
    • HEPES 0.05 M pH 7.5
    pH 7.5
    32 μl of Magnesium chloride (2.5 M)
    50 μl of HEPES pH 7.5 (1 M)
    918 μl of water (100 v/v)
    F5
    • Sodium chloride 0.2 M -
    • BIS-TRIS propane 0.05 M pH 7
    pH 7
    200 μl of Sodium chloride (1 M)
    50 μl of BIS-TRIS propane pH 7 (1 M)
    750 μl of water (100 v/v)
    F6
    • Sodium chloride 0.4 M -
    • BIS-TRIS propane 0.05 M pH 6.8
    pH 6.8
    400 μl of Sodium chloride (1 M)
    50 μl of BIS-TRIS propane pH 6.8 (1 M)
    550 μl of water (100 v/v)
    F7
    • Sodium chloride 0.6 M -
    • MES 0.05 M pH 6
    • Ammonium acetate 0.1 M -
    • Magnesium sulfate 0.005 M -
    pH 6
    5 μl of Magnesium sulfate (1 M)
    120 μl of Sodium chloride (5 M)
    100 μl of Ammonium acetate (1 M)
    50 μl of MES pH 6 (1 M)
    725 μl of water (100 v/v)
    F8
    • Sodium chloride 2.5 M -
    • tris chloride BCC 0.05 M pH 7.5
    • Magnesium chloride 0.2 M -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Sodium chloride (5 M)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    370 μl of water (100 v/v)
    F9
    • Sodium formate 1.8 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    360 μl of Sodium formate (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    540 μl of water (100 v/v)
    F10
    • Sodium formate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    400 μl of Sodium formate (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    F11
    • di-Sodium hydrogen phosphate 0.1 M -
    • Sodium chloride 0.08 M -
    NA
    80 μl of Sodium chloride (1 M)
    20 μl of di-Sodium hydrogen phosphate (5 M)
    900 μl of water (100 v/v)
    F12
    • di-Sodium tartrate 1 M -
    • tris chloride BCC 0.05 M pH 7.5
    • Magnesium chloride 0.03 M -
    pH 7.5
    12 μl of Magnesium chloride (2.5 M)
    500 μl of di-Sodium tartrate (2 M)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    438 μl of water (100 v/v)
    G1
    • 1,6-Hexanediol 10 w/v -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.02 M -
    pH 6.5
    8 μl of Magnesium chloride (2.5 M)
    200 μl of 1,6-Hexanediol (50 w/v)
    50 μl of MES pH 6.5 (1 M)
    742 μl of water (100 v/v)
    G2
    • 1,6-Hexanediol 20 w/v -
    • HEPES 0.05 M pH 7
    • Ammonium chloride 0.05 M -
    • Magnesium chloride 0.01 M -
    pH 7
    4 μl of Magnesium chloride (2.5 M)
    10 μl of Ammonium chloride (5 M)
    400 μl of 1,6-Hexanediol (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    536 μl of water (100 v/v)
    G3
    • 1,6-Hexanediol 35 w/v -
    • tris chloride BCC 0.05 M pH 8.5
    • Magnesium sulfate 0.075 M -
    pH 8.5
    75 μl of Magnesium sulfate (1 M)
    700 μl of 1,6-Hexanediol (50 w/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    175 μl of water (100 v/v)
    G4
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • tris chloride BCC 0.05 M pH 7.5
    • Ammonium acetate 0.05 M -
    pH 7.5
    50 μl of Ammonium acetate (1 M)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    700 μl of water (100 v/v)
    G5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • HEPES 0.05 M pH 7
    • Potassium chloride 0.08 M -
    • Magnesium sulfate 0.01 M -
    pH 7
    10 μl of Magnesium sulfate (1 M)
    80 μl of Potassium chloride (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    50 μl of HEPES pH 7 (1 M)
    660 μl of water (100 v/v)
    G6
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • ADA 0.05 M pH 6.5
    • Sodium acetate 0.1 M -
    pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    50 μl of Sodium acetate (2 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    600 μl of water (100 v/v)
    G7
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • di-Sodium succinate 0.05 M pH 5.5
    • Magnesium acetate 0.01 M -
    pH 5.5
    10 μl of Magnesium acetate (1 M)
    16 μl of di-Sodium succinate pH 5.5 (3 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    673 μl of water (100 v/v)
    G8
    • 2-methyl-2,4-pentanediol BCC 18 v/v -
    • MES 0.02 M pH 5.8
    • Magnesium chloride 0.01 M -
    pH 5.8
    4 μl of Magnesium chloride (2.5 M)
    20 μl of MES pH 5.8 (1 M)
    360 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    616 μl of water (100 v/v)
    G9
    • 2-methyl-2,4-pentanediol BCC 23 v/v -
    • MES 0.05 M pH 6
    • Sodium chloride 0.1 M -
    pH 6
    100 μl of Sodium chloride (1 M)
    50 μl of MES pH 6 (1 M)
    230 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    620 μl of water (100 v/v)
    G10
    • 2-methyl-2,4-pentanediol BCC 26 v/v -
    • PIPES 0.05 M pH 7
    • Magnesium chloride 0.065 M -
    • Cobalt (III) Hexamine chloride 0.001 M -
    pH 7
    10 μl of Cobalt (III) Hexamine chloride (0.1 M)
    26 μl of Magnesium chloride (2.5 M)
    50 μl of PIPES pH 7 (1 M)
    260 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    654 μl of water (100 v/v)
    G11
    • 2-methyl-2,4-pentanediol BCC 27 v/v -
    • MES 0.02 M pH 5.8
    • Sodium chloride 0.4 M -
    • Calcium chloride 0.12 M -
    pH 5.8
    400 μl of Sodium chloride (1 M)
    120 μl of Calcium chloride (1 M)
    20 μl of MES pH 5.8 (1 M)
    270 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    190 μl of water (100 v/v)
    G12
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • BIS-TRIS 0.02 M pH 6
    • Sodium chloride 0.05 M -
    • Calcium chloride 0.01 M -
    pH 6
    50 μl of Sodium chloride (1 M)
    20 μl of BIS-TRIS pH 6 (1 M)
    10 μl of Calcium chloride (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    570 μl of water (100 v/v)
    H1
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium chloride 0.01 M -
    pH 5.6
    4 μl of Magnesium chloride (2.5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    396 μl of water (100 v/v)
    H2
    • 2-Propanol 5 v/v -
    • tris chloride BCC 0.05 M pH 7.5
    • Magnesium chloride 0.01 M -
    pH 7.5
    4 μl of Magnesium chloride (2.5 M)
    50 μl of 2-Propanol (100 v/v)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    896 μl of water (100 v/v)
    H3
    • 2-Propanol 5 v/v -
    • MES 0.05 M pH 6.5
    • Calcium acetate 0.1 M -
    pH 6.5
    50 μl of 2-Propanol (100 v/v)
    100 μl of Calcium acetate (1 M)
    50 μl of MES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    H4
    • 2-Propanol 9 v/v -
    • Imidazole 0.05 M pH 7.2
    • Magnesium acetate 0.015 M -
    • Magnesium chloride 0.015 M -
    pH 7.2
    15 μl of Magnesium acetate (1 M)
    6 μl of Magnesium chloride (2.5 M)
    90 μl of 2-Propanol (100 v/v)
    50 μl of Imidazole pH 7.2 (1 M)
    839 μl of water (100 v/v)
    H5
    • 2-Propanol 10 v/v -
    • MES 0.05 M pH 6.5
    • Ammonium acetate 0.08 M -
    pH 6.5
    100 μl of 2-Propanol (100 v/v)
    80 μl of Ammonium acetate (1 M)
    50 μl of MES pH 6.5 (1 M)
    770 μl of water (100 v/v)
    H6
    • 2-Propanol 10 v/v -
    • di-Sodium succinate 0.05 M pH 5.5
    • Cobalt (II) chloride 0.002 M -
    pH 5.5
    100 μl of 2-Propanol (100 v/v)
    2 μl of Cobalt (II) chloride (1 M)
    16 μl of di-Sodium succinate pH 5.5 (3 M)
    881 μl of water (100 v/v)
    H7
    • 2-Propanol 13 v/v -
    • MOPS 0.05 M pH 7
    • Potassium chloride 0.2 M -
    • Cobalt (III) Hexamine chloride 0.006 M -
    pH 7
    60 μl of Cobalt (III) Hexamine chloride (0.1 M)
    50 μl of MOPS pH 7 (1 M)
    200 μl of Potassium chloride (1 M)
    130 μl of 2-Propanol (100 v/v)
    560 μl of water (100 v/v)
    H8
    • 2-Propanol 15 v/v -
    • MES 0.05 M pH 6
    • Magnesium chloride 0.02 M -
    pH 6
    8 μl of Magnesium chloride (2.5 M)
    150 μl of 2-Propanol (100 v/v)
    50 μl of MES pH 6 (1 M)
    792 μl of water (100 v/v)
    H9
    • 1,4-Dioxane 10 v/v -
    • HEPES 0.05 M pH 7.5
    pH 7.5
    200 μl of 1,4-Dioxane (50 v/v)
    50 μl of HEPES pH 7.5 (1 M)
    750 μl of water (100 v/v)
    H10
    • Ethanol 10 v/v -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.02 M -
    • Cobalt (II) chloride 0.001 M -
    pH 6.5
    8 μl of Magnesium chloride (2.5 M)
    200 μl of Ethanol (50 v/v)
    1 μl of Cobalt (II) chloride (1 M)
    50 μl of MES pH 6.5 (1 M)
    741 μl of water (100 v/v)
    H11
    • Ethylene glycol 20 v/v -
    • Polyethylene glycol 3,350 5 w/v -
    • Magnesium chloride 0.02 M -
    NA
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Ethylene glycol (50 v/v)
    8 μl of Magnesium chloride (2.5 M)
    492 μl of water (100 v/v)
    H12
    • Glycerol 15 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 0.2 M -
    pH 4.6
    200 μl of Sodium chloride (1 M)
    300 μl of Glycerol (50 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)