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Supplemental Information

Screen Description

PACT 2

PACT / PACT 2
Created 2009-11-16.

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PACT 2

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Screen Structure

PACT 2

Well Count 24
Number of distinct ingredients 12
Internal diversity (score mean dist) 0.761

Screen Composition

2025-08-16T06:25:33.646172 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PACT 2
Buffer 26
Polymer 24
Salt 12

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PACT 2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 4
pH 4
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 4 (1 M)
400.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 5
pH 5
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 5 (1 M)
400.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 6
pH 6
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 6 (1 M)
400.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 7
pH 7
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 7 (1 M)
400.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 8
pH 8
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 8 (1 M)
400.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 1,500 25 w/v -
  • TBG Buffer 0.1 M pH 9
pH 9
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of TBG Buffer pH 9 (1 M)
400.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Ammonium chloride 0.2 M -
pH 7
40.0 μl of Ammonium chloride (5 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
460.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Lithium chloride 0.2 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
40.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
460.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Magnesium chloride 0.2 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
420.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Calcium chloride 0.2 M -
pH 7
200.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Zinc chloride 0.01 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of Zinc chloride (1 M)
490.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 4
pH 4
100.0 μl of MMT buffer pH 4 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 5
pH 5
100.0 μl of MMT buffer pH 5 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 6
pH 6
100.0 μl of MMT buffer pH 6 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 7
pH 7
100.0 μl of MMT buffer pH 7 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 8
pH 8
100.0 μl of MMT buffer pH 8 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 9
pH 9
100.0 μl of MMT buffer pH 9 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.2 M -
pH 8
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
460.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Ammonium chloride 0.2 M -
pH 8
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
460.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Lithium chloride 0.2 M -
pH 8
40.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
460.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium chloride 0.2 M -
pH 8
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
420.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Calcium chloride 0.2 M -
pH 8
200.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Zinc chloride 0.01 M -
pH 8
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of Zinc chloride (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
490.0 μl of water (100 v/v)