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Supplemental Information

Screen Description

PACT 2

PACT / PACT 2
Created 2009-11-16.

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PACT 2

    Screen Structure

    PACT 2

    Well Count 24
    Number of distinct ingredients 12
    Internal diversity (score mean dist) 0.761

    Screen Composition

    2025-06-14T13:41:23.866104 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PACT 2
    Buffer 26
    Polymer 24
    Salt 12

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PACT 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 4
    pH 4
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of TBG Buffer pH 4 (1 M)
    400 μl of water (100 v/v)
    A2
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 5
    pH 5
    100 μl of TBG Buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A3
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 6
    pH 6
    100 μl of TBG Buffer pH 6 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A4
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 7
    pH 7
    100 μl of TBG Buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A5
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 8
    pH 8
    100 μl of TBG Buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A6
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 9
    pH 9
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of TBG Buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    B1
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    B2
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Ammonium chloride 0.2 M -
    pH 7
    40 μl of Ammonium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    B3
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Lithium chloride 0.2 M -
    pH 7
    40 μl of Lithium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    B4
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    420 μl of water (100 v/v)
    B5
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.2 M -
    pH 7
    200 μl of Calcium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    B6
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Zinc chloride 0.01 M -
    pH 7
    10 μl of Zinc chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    490 μl of water (100 v/v)
    C1
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 4
    pH 4
    100 μl of MMT buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C2
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 5
    pH 5
    100 μl of MMT buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C3
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 6
    pH 6
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 6 (1 M)
    400 μl of water (100 v/v)
    C4
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 7
    pH 7
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 7 (1 M)
    400 μl of water (100 v/v)
    C5
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 8
    pH 8
    100 μl of MMT buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 9
    pH 9
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    D1
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.2 M -
    pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    D2
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium chloride 0.2 M -
    pH 8
    40 μl of Ammonium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    D3
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium chloride 0.2 M -
    pH 8
    40 μl of Lithium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    D4
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Magnesium chloride 0.2 M -
    pH 8
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    420 μl of water (100 v/v)
    D5
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Calcium chloride 0.2 M -
    pH 8
    200 μl of Calcium chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    D6
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Zinc chloride 0.01 M -
    pH 8
    10 μl of Zinc chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    490 μl of water (100 v/v)