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Supplemental Information

Screen Description

PACT HTS

PACT / PACT HTS
Created 2009-11-16.

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PACT HTS

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PACT HTS

    Screen Structure

    PACT HTS

    Well Count 96
    Number of distinct ingredients 30
    Internal diversity (score mean dist) 0.847

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:23:21.360065 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PACT HTS
    Buffer 107
    Polymer 96
    Salt 77

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PACT HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 4
    		pH 4
    100 μl of SPG buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A2
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 5
    		pH 5
    100 μl of SPG buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A3
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 6
    		pH 6
    100 μl of SPG buffer pH 6 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A4
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 7
    		pH 7
    100 μl of SPG buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A5
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 8
    		pH 8
    100 μl of SPG buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A6
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 9
    		pH 9
    100 μl of SPG buffer pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    A7
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Sodium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    40 μl of Sodium chloride (5 M)
    526 μl of water (100 v/v)
    A8
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Ammonium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    40 μl of Ammonium chloride (5 M)
    526 μl of water (100 v/v)
    A9
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Lithium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    40 μl of Lithium chloride (5 M)
    526 μl of water (100 v/v)
    A10
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Magnesium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    80 μl of Magnesium chloride (2.5 M)
    486 μl of water (100 v/v)
    A11
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Calcium chloride 0.2 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    40 μl of Calcium chloride (5 M)
    526 μl of water (100 v/v)
    A12
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 5
    • Zinc chloride 0.01 M -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of Sodium acetate pH 5 (3 M)
    10 μl of Zinc chloride (1 M)
    556 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 4
    		pH 4
    100 μl of MIB buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B2
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 5
    		pH 5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MIB buffer pH 5 (1 M)
    400 μl of water (100 v/v)
    B3
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 6
    		pH 6
    100 μl of MIB buffer pH 6 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B4
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 7
    		pH 7
    100 μl of MIB buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B5
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 8
    		pH 8
    100 μl of MIB buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B6
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 9
    		pH 9
    100 μl of MIB buffer pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B7
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Sodium chloride 0.2 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    33 μl of MES pH 6 (3 M)
    526 μl of water (100 v/v)
    B8
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Ammonium chloride 0.2 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Ammonium chloride (5 M)
    33 μl of MES pH 6 (3 M)
    526 μl of water (100 v/v)
    B9
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Lithium chloride 0.2 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Lithium chloride (5 M)
    33 μl of MES pH 6 (3 M)
    526 μl of water (100 v/v)
    B10
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Magnesium chloride 0.2 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    33 μl of MES pH 6 (3 M)
    486 μl of water (100 v/v)
    B11
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Calcium chloride 0.2 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Calcium chloride (5 M)
    33 μl of MES pH 6 (3 M)
    526 μl of water (100 v/v)
    B12
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Zinc chloride 0.01 M -
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    10 μl of Zinc chloride (1 M)
    33 μl of MES pH 6 (3 M)
    556 μl of water (100 v/v)
    C1
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 4
    		pH 4
    100 μl of TBG Buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C2
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 5
    		pH 5
    100 μl of TBG Buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C3
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 6
    		pH 6
    100 μl of TBG Buffer pH 6 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C4
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 7
    		pH 7
    100 μl of TBG Buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C5
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 8
    		pH 8
    100 μl of TBG Buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Polyethylene glycol 1,500 25 w/v -
    • TBG Buffer 0.1 M pH 9
    		pH 9
    100 μl of TBG Buffer pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    C7
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    C8
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Ammonium chloride 0.2 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    40 μl of Ammonium chloride (5 M)
    460 μl of water (100 v/v)
    C9
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Lithium chloride 0.2 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    40 μl of Lithium chloride (5 M)
    460 μl of water (100 v/v)
    C10
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7 (1 M)
    420 μl of water (100 v/v)
    C11
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.2 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    200 μl of Calcium chloride (1 M)
    300 μl of water (100 v/v)
    C12
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Zinc chloride 0.01 M -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    10 μl of Zinc chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    490 μl of water (100 v/v)
    D1
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 4
    		pH 4
    100 μl of MMT buffer pH 4 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D2
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 5
    		pH 5
    100 μl of MMT buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D3
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 6
    		pH 6
    100 μl of MMT buffer pH 6 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D4
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 7
    		pH 7
    100 μl of MMT buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 8
    		pH 8
    100 μl of MMT buffer pH 8 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D6
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 9
    		pH 9
    100 μl of MMT buffer pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    D7
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    460 μl of water (100 v/v)
    D8
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium chloride 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    40 μl of Ammonium chloride (5 M)
    460 μl of water (100 v/v)
    D9
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium chloride 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Lithium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    460 μl of water (100 v/v)
    D10
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Magnesium chloride 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    420 μl of water (100 v/v)
    D11
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Calcium chloride 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Calcium chloride (1 M)
    300 μl of water (100 v/v)
    D12
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Zinc chloride 0.01 M -
    		pH 8
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    10 μl of Zinc chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    490 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of Sodium fluoride (0.8 M)
    350 μl of water (100 v/v)
    E2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium bromide 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium bromide (5 M)
    560 μl of water (100 v/v)
    E3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium iodide 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium iodide (2 M)
    500 μl of water (100 v/v)
    E4
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium thiocyanate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    575 μl of water (100 v/v)
    E5
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium nitrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium nitrate (7 M)
    571 μl of water (100 v/v)
    E6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium formate (5 M)
    560 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.2 M -
    		NA
    100 μl of Sodium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium sulfate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    400 μl of water (100 v/v)
    E9
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    		NA
    200 μl of Potassium Sodium tartrate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    E10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium Potassium phosphate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium Potassium phosphate (1 M)
    400 μl of water (100 v/v)
    E11
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate (2 M)
    500 μl of water (100 v/v)
    E12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Sodium malonate (2 M)
    500 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium fluoride 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    250 μl of Sodium fluoride (0.8 M)
    250 μl of water (100 v/v)
    F2
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium bromide 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium bromide (5 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    460 μl of water (100 v/v)
    F3
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium iodide 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium iodide (2 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of water (100 v/v)
    F4
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Potassium thiocyanate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    475 μl of water (100 v/v)
    F5
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium nitrate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    F6
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium formate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    40 μl of Sodium formate (5 M)
    460 μl of water (100 v/v)
    F7
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium acetate 0.2 M -
    		pH 6.5
    100 μl of Sodium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of water (100 v/v)
    F8
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium sulfate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F9
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Potassium Sodium tartrate 0.2 M -
    		pH 6.5
    200 μl of Potassium Sodium tartrate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F10
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium Potassium phosphate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium Potassium phosphate (1 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F11
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • tri-Sodium citrate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate (2 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of water (100 v/v)
    F12
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • di-Sodium malonate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    100 μl of di-Sodium malonate (2 M)
    400 μl of water (100 v/v)
    G1
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium fluoride 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    250 μl of Sodium fluoride (0.8 M)
    250 μl of water (100 v/v)
    G2
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium bromide 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium bromide (5 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    460 μl of water (100 v/v)
    G3
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium iodide 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium iodide (2 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of water (100 v/v)
    G4
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Potassium thiocyanate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    475 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium nitrate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    G6
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium formate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium formate (7 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    471 μl of water (100 v/v)
    G7
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium acetate 0.2 M -
    		pH 7.5
    100 μl of Sodium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of water (100 v/v)
    G8
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Sodium sulfate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Sodium sulfate (1 M)
    300 μl of water (100 v/v)
    G9
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.2 M -
    		pH 7.5
    200 μl of Potassium Sodium tartrate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    300 μl of water (100 v/v)
    G10
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    500 μl of water (100 v/v)
    G11
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • tri-Sodium citrate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate (2 M)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    400 μl of water (100 v/v)
    G12
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • di-Sodium malonate 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    100 μl of di-Sodium malonate (2 M)
    400 μl of water (100 v/v)
    H1
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium fluoride 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    250 μl of Sodium fluoride (0.8 M)
    250 μl of water (100 v/v)
    H2
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium bromide 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    40 μl of Sodium bromide (5 M)
    460 μl of water (100 v/v)
    H3
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium iodide 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium iodide (2 M)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H4
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Potassium thiocyanate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    475 μl of water (100 v/v)
    H5
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium nitrate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    28 μl of Sodium nitrate (7 M)
    471 μl of water (100 v/v)
    H6
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium formate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    40 μl of Sodium formate (5 M)
    460 μl of water (100 v/v)
    H7
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium acetate 0.2 M -
    		pH 8.5
    100 μl of Sodium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H8
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium sulfate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    200 μl of Sodium sulfate (1 M)
    300 μl of water (100 v/v)
    H9
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Potassium Sodium tartrate 0.2 M -
    		pH 8.5
    200 μl of Potassium Sodium tartrate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H10
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • Sodium Potassium phosphate 0.02 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Sodium Potassium phosphate (1 M)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    480 μl of water (100 v/v)
    H11
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • tri-Sodium citrate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate (2 M)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H12
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • di-Sodium malonate 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    100 μl of di-Sodium malonate (2 M)
    400 μl of water (100 v/v)