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Supplemental Information

Screen Description

Phosphatase 1

Phosphatase / Phosphatase 1
Created 2009-11-16.

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Phosphatase 1

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Screen Structure

Phosphatase 1

Well Count 24
Number of distinct ingredients 39
Internal diversity (score mean dist) 0.907

Screen Composition

2025-08-16T09:05:43.410705 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Phosphatase 1
Buffer 28
Cryoprotectant 1
Organic (non-volatile) 5
Organic (volatile) 1
Polymer 24
Salt 29

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Phosphatase 1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 15 v/v -
  • tri-Sodium citrate 0.05 M pH 5.6
  • Ammonium sulfate 0.2 M -
  • di-Sodium tartrate 0.2 M -
pH 5.6
66.7 μl of Ammonium sulfate (3 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Sodium tartrate (2 M)
25.0 μl of tri-Sodium citrate pH 5.6 (2 M)
658.3 μl of water (100 v/v)
A2
  • Polyethylene glycol 400 22 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
220.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
613.3 μl of water (100 v/v)
A3
  • Polyethylene glycol 400 25 v/v -
  • HEPES 0.1 M pH 7.5
  • Cesium chloride 0.2 M -
pH 7.5
200.0 μl of Cesium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
A4
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • MES 0.1 M pH 6.5
  • Zinc sulfate 0.01 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
10.0 μl of Zinc sulfate (1 M)
640.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 1,500 27 w/v -
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • Imidazole 0.01 M pH 7
  • Sodium nitrate 0.22 M -
pH 7
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Imidazole pH 7 (1 M)
540.0 μl of Polyethylene glycol 1,500 (50 w/v)
31.4 μl of Sodium nitrate (7 M)
318.6 μl of water (100 v/v)
A6
  • Polyethylene glycol 1,500 30 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Potassium fluoride 0.4 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
66.7 μl of Potassium fluoride (6 M)
233.3 μl of water (100 v/v)
B1
  • Polyethylene glycol monomethyl ether 2,000 8 w/v -
  • tris chloride BCC 0.01 M pH 7
  • Nickel (II) chloride 0.01 M -
pH 7
10.0 μl of Nickel (II) chloride pH 7 (1 M)
160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
3.3 μl of tris chloride BCC pH 7 (3 M)
826.7 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.4 M -
pH 4.6
133.3 μl of Ammonium sulfate (3 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
446.7 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 8 w/v -
  • Sodium acetate 0.1 M -
NA
160.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate (2 M)
790.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.2 M -
pH 7.5
40.0 μl of Ammonium acetate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
660.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.05 M -
  • Sodium chloride 0.015 M -
pH 7.5
10.0 μl of Ammonium acetate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
15.0 μl of Sodium chloride (1 M)
675.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 10 w/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.02 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Zinc acetate (2 M)
690.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 3,350 15 w/v -
  • Sodium fluoride 0.2 M -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
450.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 3,350 15 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Glycine 0.05 M -
  • Sodium formate 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
25.0 μl of Glycine (2 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
546.4 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 15 w/v -
  • Glycerol 5 v/v -
  • BIS-TRIS propane 0.1 M pH 7
  • Magnesium formate 0.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
50.0 μl of Glycerol (100 v/v)
200.0 μl of Magnesium formate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Potassium fluoride (6 M)
566.7 μl of water (100 v/v)
C5
  • Polyethylene glycol 3,350 20 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium chloride 0.1 M -
pH 6.5
20.0 μl of Ammonium chloride (5 M)
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
480.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 3,350 20 w/v -
  • tris chloride BCC 0.01 M pH 8
  • Potassium sulfate 0.2 M -
pH 8
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium sulfate (0.5 M)
10.0 μl of tris chloride BCC pH 8 (1 M)
190.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.1 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of tri-Sodium citrate (2 M)
550.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 3,350 22 w/v -
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 0.2 M -
pH 7.5
40.0 μl of Calcium chloride (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol 3,350 (50 w/v)
420.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 3,350 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 25 w/v -
  • 2-Propanol 10 v/v -
  • tri-Sodium citrate 0.1 M pH 4.8
  • Lithium chloride 0.1 M -
pH 4.8
100.0 μl of 2-Propanol (100 v/v)
20.0 μl of Lithium chloride (5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.8 (1 M)
280.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,350 30 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate (10 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
380.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 30 w/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
NA
66.7 μl of Ammonium di-hydrogen phosphate (3 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)