C6 - Screen Contents - Phosphatase 1

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Supplemental Information

Screen Description

Phosphatase 1

Phosphatase / Phosphatase 1

Created 2009-11-16.

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Phosphatase 1

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Phosphatase 1

Screen Structure

Phosphatase 1

Well Count	24
Number of distinct ingredients	39
Internal diversity (score mean dist)	0.907

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase 1
Buffer	28
Cryoprotectant	1
Organic (non-volatile)	5
Organic (volatile)	1
Polymer	24
Salt	29

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 400 15 v/v - tri-Sodium citrate 0.05 M pH 5.6 Ammonium sulfate 0.2 M - di-Sodium tartrate 0.2 M - 🔍	pH 5.6	100 μl of di-Sodium tartrate (2 M) 25 μl of tri-Sodium citrate pH 5.6 (2 M) 150 μl of Polyethylene glycol 400 (100 v/v) 66 μl of Ammonium sulfate (3 M) 658 μl of water (100 v/v)
A2	Polyethylene glycol 400 22 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Lithium sulfate (3 M) 220 μl of Polyethylene glycol 400 (100 v/v) 613 μl of water (100 v/v)
A3	Polyethylene glycol 400 25 v/v - HEPES 0.1 M pH 7.5 Cesium chloride 0.2 M - 🔍	pH 7.5	200 μl of Cesium chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 250 μl of Polyethylene glycol 400 (100 v/v) 450 μl of water (100 v/v)
A4	Polyethylene glycol monomethyl ether 550 25 v/v - MES 0.1 M pH 6.5 Zinc sulfate 0.01 M - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 10 μl of Zinc sulfate (1 M) 250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 640 μl of water (100 v/v)
A5	Polyethylene glycol 1,500 27 w/v - 2-methyl-2,4-pentanediol BCC 10 v/v - Imidazole 0.01 M pH 7 Sodium nitrate 0.22 M - 🔍	pH 7	31 μl of Sodium nitrate (7 M) 10 μl of Imidazole pH 7 (1 M) 540 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 318 μl of water (100 v/v)
A6	Polyethylene glycol 1,500 30 w/v - BIS-TRIS propane 0.1 M pH 6.5 Potassium fluoride 0.4 M - 🔍	pH 6.5	66 μl of Potassium fluoride (6 M) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 600 μl of Polyethylene glycol 1,500 (50 w/v) 233 μl of water (100 v/v)
B1	Polyethylene glycol monomethyl ether 2,000 8 w/v - tris chloride BCC 0.01 M pH 7 Nickel (II) chloride 0.01 M - 🔍	pH 7	10 μl of Nickel (II) chloride pH 7 (1 M) 160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 3 μl of tris chloride BCC pH 7 (3 M) 826 μl of water (100 v/v)
B2	Polyethylene glycol monomethyl ether 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 0.4 M - 🔍	pH 4.6	320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 133 μl of Ammonium sulfate (3 M) 446 μl of water (100 v/v)
B3	Polyethylene glycol 3,350 8 w/v - Sodium acetate 0.1 M - 🔍	NA	160 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Sodium acetate (2 M) 790 μl of water (100 v/v)
B4	Polyethylene glycol 3,350 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Ammonium acetate (5 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 660 μl of water (100 v/v)
B5	Polyethylene glycol 3,350 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.05 M - Sodium chloride 0.015 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 10 μl of Ammonium acetate (5 M) 15 μl of Sodium chloride (1 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 675 μl of water (100 v/v)
B6	Polyethylene glycol 3,350 10 w/v - MES 0.1 M pH 6.5 Zinc acetate 0.02 M - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 10 μl of Zinc acetate (2 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 690 μl of water (100 v/v)
C1	Polyethylene glycol 3,350 15 w/v - Sodium fluoride 0.2 M - 🔍	NA	250 μl of Sodium fluoride (0.8 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 450 μl of water (100 v/v)
C2	Polyethylene glycol 3,350 15 w/v - BIS-TRIS propane 0.1 M pH 6.5 Glycine 0.05 M - Sodium formate 0.2 M - 🔍	pH 6.5	28 μl of Sodium formate (7 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 25 μl of Glycine (2 M) 546 μl of water (100 v/v)
C3	Polyethylene glycol 3,350 15 w/v - Glycerol 5 v/v - BIS-TRIS propane 0.1 M pH 7 Magnesium formate 0.2 M - 🔍	pH 7	200 μl of Magnesium formate (1 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS propane pH 7 (1 M) 50 μl of Glycerol (100 v/v) 350 μl of water (100 v/v)
C4	Polyethylene glycol 3,350 20 w/v - Potassium fluoride 0.2 M - 🔍	NA	33 μl of Potassium fluoride (6 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 566 μl of water (100 v/v)
C5	Polyethylene glycol 3,350 20 w/v - MES 0.1 M pH 6.5 Ammonium chloride 0.1 M - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium chloride (5 M) 480 μl of water (100 v/v)
C6	Polyethylene glycol 3,350 20 w/v - tris chloride BCC 0.01 M pH 8 Potassium sulfate 0.2 M - 🔍	pH 8	400 μl of Polyethylene glycol 3,350 (50 w/v) 10 μl of tris chloride BCC pH 8 (1 M) 400 μl of Potassium sulfate (0.5 M) 190 μl of water (100 v/v)
D1	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.1 M - 🔍	NA	50 μl of tri-Sodium citrate (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 550 μl of water (100 v/v)
D2	Polyethylene glycol 3,350 22 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Calcium chloride (5 M) 440 μl of Polyethylene glycol 3,350 (50 w/v) 420 μl of water (100 v/v)
D3	Polyethylene glycol 3,350 25 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of Magnesium chloride pH 7 (2.5 M) 320 μl of water (100 v/v)
D4	Polyethylene glycol 3,350 25 w/v - 2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 4.8 Lithium chloride 0.1 M - 🔍	pH 4.8	100 μl of 2-Propanol (100 v/v) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 4.8 (1 M) 20 μl of Lithium chloride (5 M) 280 μl of water (100 v/v)
D5	Polyethylene glycol 3,350 30 w/v - Ammonium nitrate 0.2 M - 🔍	NA	600 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium nitrate (10 M) 380 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 30 w/v - Ammonium di-hydrogen phosphate 0.2 M - 🔍	NA	600 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of Ammonium di-hydrogen phosphate (3 M) 333 μl of water (100 v/v)