Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Phosphatase 1
Barcodes
Phosphatase 1
No barcodes found.
Screen Structure
Phosphatase 1
Well Count | 24 |
---|---|
Number of distinct ingredients | 39 |
Internal diversity (score mean dist) | 0.907 |
Screen Composition
ingredient type | Phosphatase 1 |
---|---|
Buffer | 28 |
Cryoprotectant | 1 |
Organic (non-volatile) | 5 |
Organic (volatile) | 1 |
Polymer | 24 |
Salt | 29 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.6 | 66.7 μl of Ammonium sulfate (3 M) 150.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of di-Sodium tartrate (2 M) 25.0 μl of tri-Sodium citrate pH 5.6 (2 M) 658.3 μl of water (100 v/v) |
A2 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 220.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 613.3 μl of water (100 v/v) |
A3 |
| pH 7.5 | 200.0 μl of Cesium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 450.0 μl of water (100 v/v) |
A4 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 10.0 μl of Zinc sulfate (1 M) 640.0 μl of water (100 v/v) |
A5 |
| pH 7 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Imidazole pH 7 (1 M) 540.0 μl of Polyethylene glycol 1,500 (50 w/v) 31.4 μl of Sodium nitrate (7 M) 318.6 μl of water (100 v/v) |
A6 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 66.7 μl of Potassium fluoride (6 M) 233.3 μl of water (100 v/v) |
B1 |
| pH 7 | 10.0 μl of Nickel (II) chloride pH 7 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 3.3 μl of tris chloride BCC pH 7 (3 M) 826.7 μl of water (100 v/v) |
B2 |
| pH 4.6 | 133.3 μl of Ammonium sulfate (3 M) 320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 446.7 μl of water (100 v/v) |
B3 |
| NA | 160.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Sodium acetate (2 M) 790.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 40.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 660.0 μl of water (100 v/v) |
B5 |
| pH 7.5 | 10.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 15.0 μl of Sodium chloride (1 M) 675.0 μl of water (100 v/v) |
B6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 10.0 μl of Zinc acetate (2 M) 690.0 μl of water (100 v/v) |
C1 |
| NA | 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 450.0 μl of water (100 v/v) |
C2 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 25.0 μl of Glycine (2 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium formate (7 M) 546.4 μl of water (100 v/v) |
C3 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 50.0 μl of Glycerol (100 v/v) 200.0 μl of Magnesium formate (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
C4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of Potassium fluoride (6 M) 566.7 μl of water (100 v/v) |
C5 |
| pH 6.5 | 20.0 μl of Ammonium chloride (5 M) 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 480.0 μl of water (100 v/v) |
C6 |
| pH 8 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of Potassium sulfate (0.5 M) 10.0 μl of tris chloride BCC pH 8 (1 M) 190.0 μl of water (100 v/v) |
D1 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of tri-Sodium citrate (2 M) 550.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 40.0 μl of Calcium chloride (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 440.0 μl of Polyethylene glycol 3,350 (50 w/v) 420.0 μl of water (100 v/v) |
D3 |
| pH 8.5 | 80.0 μl of Magnesium chloride pH 7 (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
D4 |
| pH 4.8 | 100.0 μl of 2-Propanol (100 v/v) 20.0 μl of Lithium chloride (5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4.8 (1 M) 280.0 μl of water (100 v/v) |
D5 |
| NA | 20.0 μl of Ammonium nitrate (10 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 380.0 μl of water (100 v/v) |
D6 |
| NA | 66.7 μl of Ammonium di-hydrogen phosphate (3 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |