C6 - Screen Contents - Phosphatase 2

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Supplemental Information

Screen Description

Phosphatase 2

Phosphatase / Phosphatase 2

Created 2009-11-16.

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Phosphatase 2

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Phosphatase 2

Screen Structure

Phosphatase 2

Well Count	24
Number of distinct ingredients	32
Internal diversity (score mean dist)	0.894

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase 2
Buffer	30
Cryoprotectant	2
Organic (non-volatile)	4
Organic (volatile)	4
Polymer	25
Salt	24

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 4,000 8 w/v - HEPES 0.05 M pH 7 Lithium sulfate 0.022 M - ß-Mercaptoethanol 0.1 v/v - 🔍	pH 7	50 μl of HEPES pH 7 (1 M) 7 μl of Lithium sulfate (3 M) 50 μl of ß-Mercaptoethanol (2 v/v) 160 μl of Polyethylene glycol 4,000 (50 w/v) 732 μl of water (100 v/v)
A2	Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.05 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 10 μl of Ammonium acetate (5 M) 690 μl of water (100 v/v)
A3	Polyethylene glycol 4,000 10 w/v - Glycerol 10 v/v - di-Sodium succinate 0.05 M pH 5.5 Magnesium chloride 0.003 M - TRIS-Phosphate 0.05 M - 🔍	pH 5.5	100 μl of Glycerol (100 v/v) 1 μl of Magnesium chloride pH 7 (2.5 M) 16 μl of di-Sodium succinate pH 5.5 (3 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of TRIS-Phosphate (2 M) 657 μl of water (100 v/v)
A4	Polyethylene glycol 4,000 12 w/v - tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - 🔍	pH 5.6	25 μl of tri-Sodium citrate pH 5.6 (4 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of 2-Propanol (100 v/v) 635 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 12 w/v - Imidazole-Malate 0.2 M pH 6 Zinc acetate 0.002 M - 🔍	pH 6	100 μl of Imidazole-Malate pH 6 (2 M) 1 μl of Zinc acetate (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 659 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 15 w/v - HEPES 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 80 μl of Magnesium chloride pH 7 (2.5 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 520 μl of water (100 v/v)
B1	Polyethylene glycol 4,000 15 w/v - Polyethylene glycol 400 15 v/v - MES 0.05 M pH 6.5 Magnesium sulfate 0.2 M - Ammonium sulfate 0.05 M - 🔍	pH 6.5	50 μl of MES pH 6.5 (1 M) 200 μl of Magnesium sulfate (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 150 μl of Polyethylene glycol 400 (100 v/v) 16 μl of Ammonium sulfate (3 M) 283 μl of water (100 v/v)
B2	Polyethylene glycol 4,000 15 w/v - MES 0.085 M pH 6.5 Sodium acetate 0.17 M - 🔍	pH 6.5	85 μl of MES pH 6.5 (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 85 μl of Sodium acetate (2 M) 530 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 20 w/v - BICINE 0.1 M pH 9 2-Propanol 5 v/v - 🔍	pH 9	400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 50 μl of 2-Propanol (100 v/v) 450 μl of water (100 v/v)
B4	Polyethylene glycol 4,000 22 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 2-Propanol 10 v/v - ß-Mercaptoethanol 0.5 v/v - 🔍	pH 8.5	66 μl of Lithium sulfate (3 M) 250 μl of ß-Mercaptoethanol (2 v/v) 440 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of 2-Propanol (100 v/v) 43 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 25 w/v - BIS-TRIS propane 0.1 M pH 6.5 di-Ammonium tartrate 0.2 M - 🔍	pH 6.5	100 μl of BIS-TRIS propane pH 6.5 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of di-Ammonium tartrate (2 M) 300 μl of water (100 v/v)
B6	Polyethylene glycol 4,000 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 30 w/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.2 M - 🔍	pH 5.6	600 μl of Polyethylene glycol 4,000 (50 w/v) 40 μl of Ammonium acetate (5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 260 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 7.5 Cesium chloride 0.1 M - Lithium sulfate 0.25 M - 🔍	pH 7.5	100 μl of Cesium chloride (1 M) 83 μl of Lithium sulfate (3 M) 33 μl of tris chloride BCC pH 7.5 (3 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 183 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 35 w/v - MES 0.1 M pH 6 🔍	pH 6	700 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 200 μl of water (100 v/v)
C4	Polyethylene glycol 4,000 42 w/v - tris chloride BCC 0.05 M pH 8 Sodium perchlorate 0.1 M - 🔍	pH 8	840 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium perchlorate (2 M) 50 μl of tris chloride BCC pH 8 (1 M) 60 μl of water (100 v/v)
C5	Polyethylene glycol 6,000 8 w/v - tri-Sodium citrate 0.1 M pH 4 Ammonium di-hydrogen phosphate 0.05 M - 2-Propanol 10 v/v - 🔍	pH 4	50 μl of Ammonium di-hydrogen phosphate (1 M) 160 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 4 (1 M) 100 μl of 2-Propanol (100 v/v) 590 μl of water (100 v/v)
C6	Polyethylene glycol 6,000 10 w/v - 2-methyl-2,4-pentanediol BCC 4 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 660 μl of water (100 v/v)
D1	Polyethylene glycol 6,000 10 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v)
D2	Polyethylene glycol 6,000 12 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	240 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 660 μl of water (100 v/v)
D3	Polyethylene glycol 6,000 12 w/v - TRIS-Acetate 0.1 M pH 8 🔍	pH 8	50 μl of TRIS-Acetate pH 8 (2 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 710 μl of water (100 v/v)
D4	Polyethylene glycol 6,000 12 w/v - Glycerol 2 v/v - di-Sodium hydrogen phosphate 0.025 M - 🔍	NA	20 μl of Glycerol (100 v/v) 240 μl of Polyethylene glycol 6,000 (50 w/v) 5 μl of di-Sodium hydrogen phosphate (5 M) 735 μl of water (100 v/v)
D5	Polyethylene glycol 6,000 15 w/v - Sodium di-hydrogen phosphate 0.05 M pH 7 Potassium chloride 0.025 M - Magnesium sulfate 0.005 M - 🔍	pH 7	300 μl of Polyethylene glycol 6,000 (50 w/v) 5 μl of Magnesium sulfate (1 M) 5 μl of Potassium chloride (5 M) 12 μl of Sodium di-hydrogen phosphate pH 7 (4 M) 677 μl of water (100 v/v)
D6	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 500 μl of water (100 v/v)