C6 - Screen Contents - Phosphatase 2

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Screen Description

Phosphatase 2

Phosphatase / Phosphatase 2

Created 2009-11-16.

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Phosphatase 2

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Phosphatase 2

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Screen Structure

Phosphatase 2

Well Count	24
Number of distinct ingredients	32
Internal diversity (score mean dist)	0.894

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase 2
Buffer	30
Cryoprotectant	2
Organic (non-volatile)	4
Organic (volatile)	4
Polymer	25
Salt	24

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 4,000 8 w/v - HEPES 0.05 M pH 7 Lithium sulfate 0.022 M - ß-Mercaptoethanol 0.1 v/v - 🔍	pH 7	50.0 μl of HEPES pH 7 (1 M) 7.3 μl of Lithium sulfate (3 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of ß-Mercaptoethanol (2 v/v) 732.7 μl of water (100 v/v)
A2	Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.05 M - 🔍	pH 7.5	10.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 690.0 μl of water (100 v/v)
A3	Polyethylene glycol 4,000 10 w/v - Glycerol 10 v/v - di-Sodium succinate 0.05 M pH 5.5 Magnesium chloride 0.003 M - TRIS-Phosphate 0.05 M - 🔍	pH 5.5	100.0 μl of Glycerol (100 v/v) 1.2 μl of Magnesium chloride pH 7 (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of TRIS-Phosphate (2 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 657.1 μl of water (100 v/v)
A4	Polyethylene glycol 4,000 12 w/v - tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - 🔍	pH 5.6	100.0 μl of 2-Propanol (100 v/v) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 635.0 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 12 w/v - Imidazole-Malate 0.2 M pH 6 Zinc acetate 0.002 M - 🔍	pH 6	100.0 μl of Imidazole-Malate pH 6 (2 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 1.0 μl of Zinc acetate (2 M) 659.0 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 15 w/v - HEPES 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 520.0 μl of water (100 v/v)
B1	Polyethylene glycol 4,000 15 w/v - Polyethylene glycol 400 15 v/v - MES 0.05 M pH 6.5 Magnesium sulfate 0.2 M - Ammonium sulfate 0.05 M - 🔍	pH 6.5	16.7 μl of Ammonium sulfate (3 M) 50.0 μl of MES pH 6.5 (1 M) 200.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of Polyethylene glycol 400 (100 v/v) 283.3 μl of water (100 v/v)
B2	Polyethylene glycol 4,000 15 w/v - MES 0.085 M pH 6.5 Sodium acetate 0.17 M - 🔍	pH 6.5	85.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of Sodium acetate (2 M) 530.0 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 20 w/v - BICINE 0.1 M pH 9 2-Propanol 5 v/v - 🔍	pH 9	50.0 μl of 2-Propanol (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 450.0 μl of water (100 v/v)
B4	Polyethylene glycol 4,000 22 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 2-Propanol 10 v/v - ß-Mercaptoethanol 0.5 v/v - 🔍	pH 8.5	100.0 μl of 2-Propanol (100 v/v) 66.7 μl of Lithium sulfate (3 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of ß-Mercaptoethanol (2 v/v) 43.3 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 25 w/v - BIS-TRIS propane 0.1 M pH 6.5 di-Ammonium tartrate 0.2 M - 🔍	pH 6.5	100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of di-Ammonium tartrate (2 M) 300.0 μl of water (100 v/v)
B6	Polyethylene glycol 4,000 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 300.0 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 30 w/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.2 M - 🔍	pH 5.6	40.0 μl of Ammonium acetate (5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 260.0 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 7.5 Cesium chloride 0.1 M - Lithium sulfate 0.25 M - 🔍	pH 7.5	100.0 μl of Cesium chloride (1 M) 83.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 33.3 μl of tris chloride BCC pH 7.5 (3 M) 183.3 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 35 w/v - MES 0.1 M pH 6 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 700.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v)
C4	Polyethylene glycol 4,000 42 w/v - tris chloride BCC 0.05 M pH 8 Sodium perchlorate 0.1 M - 🔍	pH 8	840.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium perchlorate (2 M) 50.0 μl of tris chloride BCC pH 8 (1 M) 60.0 μl of water (100 v/v)
C5	Polyethylene glycol 6,000 8 w/v - tri-Sodium citrate 0.1 M pH 4 Ammonium di-hydrogen phosphate 0.05 M - 2-Propanol 10 v/v - 🔍	pH 4	100.0 μl of 2-Propanol (100 v/v) 50.0 μl of Ammonium di-hydrogen phosphate (1 M) 160.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 590.0 μl of water (100 v/v)
C6	Polyethylene glycol 6,000 10 w/v - 2-methyl-2,4-pentanediol BCC 4 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 660.0 μl of water (100 v/v)
D1	Polyethylene glycol 6,000 10 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v)
D2	Polyethylene glycol 6,000 12 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	240.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 660.0 μl of water (100 v/v)
D3	Polyethylene glycol 6,000 12 w/v - TRIS-Acetate 0.1 M pH 8 🔍	pH 8	240.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of TRIS-Acetate pH 8 (2 M) 710.0 μl of water (100 v/v)
D4	Polyethylene glycol 6,000 12 w/v - Glycerol 2 v/v - di-Sodium hydrogen phosphate 0.025 M - 🔍	NA	20.0 μl of Glycerol (100 v/v) 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of di-Sodium hydrogen phosphate (5 M) 735.0 μl of water (100 v/v)
D5	Polyethylene glycol 6,000 15 w/v - Sodium di-hydrogen phosphate 0.05 M pH 7 Potassium chloride 0.025 M - Magnesium sulfate 0.005 M - 🔍	pH 7	5.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of Potassium chloride (5 M) 12.5 μl of Sodium di-hydrogen phosphate pH 7 (4 M) 677.5 μl of water (100 v/v)
D6	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)