C6 - Screen Contents - Phosphatase 3

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Supplemental Information

Screen Description

Phosphatase 3

Phosphatase / Phosphatase 3

Created 2009-11-16.

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Phosphatase 3

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Phosphatase 3

Screen Structure

Phosphatase 3

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.881

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase 3
Buffer	29
Cryoprotectant	1
Detergent	1
Organic (non-volatile)	2
Organic (volatile)	1
Polymer	26
Salt	25

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 8 w/v - Potassium di-hydrogen phosphate 0.1 M - ß-Mercaptoethanol 0.2 v/v - 🔍	NA	50 μl of Potassium di-hydrogen phosphate (2 M) 100 μl of ß-Mercaptoethanol (2 v/v) 160 μl of Polyethylene glycol 8,000 (50 w/v) 690 μl of water (100 v/v)
A2	Polyethylene glycol 8,000 10 w/v - HEPES 0.1 M pH 7.5 Magnesium acetate 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium acetate (2 M) 600 μl of water (100 v/v)
A3	Polyethylene glycol 8,000 10 w/v - MES 0.1 M pH 6.5 Potassium chloride 0.2 M - Magnesium acetate 0.1 M - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 40 μl of Potassium chloride (5 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Magnesium acetate (2 M) 610 μl of water (100 v/v)
A4	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.05 M - 🔍	pH 8.5	50 μl of Sodium chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 650 μl of water (100 v/v)
A5	Polyethylene glycol 8,000 12 w/v - Sodium acetate 0.05 M pH 5 Ammonium acetate 0.3 M - 🔍	pH 5	60 μl of Ammonium acetate (5 M) 16 μl of Sodium acetate pH 5 (3 M) 240 μl of Polyethylene glycol 8,000 (50 w/v) 683 μl of water (100 v/v)
A6	Polyethylene glycol 8,000 12 w/v - tris chloride BCC 0.1 M pH 6.3 Lithium sulfate 0.2 M - 🔍	pH 6.3	33 μl of tris chloride BCC pH 6.3 (3 M) 66 μl of Lithium sulfate (3 M) 240 μl of Polyethylene glycol 8,000 (50 w/v) 660 μl of water (100 v/v)
B1	Polyethylene glycol 8,000 12 w/v - HEPES 0.1 M pH 7.5 Sodium acetate 0.2 M - 🔍	pH 7.5	80 μl of Sodium acetate (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 240 μl of Polyethylene glycol 8,000 (50 w/v) 580 μl of water (100 v/v)
B2	Polyethylene glycol 8,000 13 w/v - Glycerol 15 v/v - MOPS 0.1 M pH 7 Ammonium sulfate 0.5 M - 🔍	pH 7	150 μl of Glycerol (100 v/v) 100 μl of MOPS pH 7 (1 M) 260 μl of Polyethylene glycol 8,000 (50 w/v) 166 μl of Ammonium sulfate (3 M) 323 μl of water (100 v/v)
B3	Polyethylene glycol 8,000 15 w/v - Potassium di-hydrogen phosphate 0.1 M pH 4.6 🔍	pH 4.6	50 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 650 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 15 w/v - Sodium di-hydrogen phosphate 0.1 M pH 4.6 🔍	pH 4.6	25 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 675 μl of water (100 v/v)
B5	Polyethylene glycol 8,000 15 w/v - di-Sodium succinate 0.1 M pH 5.5 Lithium sulfate 0.25 M - 🔍	pH 5.5	33 μl of di-Sodium succinate pH 5.5 (3 M) 83 μl of Lithium sulfate (3 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 583 μl of water (100 v/v)
B6	Polyethylene glycol 8,000 18 w/v - HEPES 0.1 M pH 7 Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 100 μl of Potassium di-hydrogen phosphate pH 7 (1 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 440 μl of water (100 v/v)
C1	Polyethylene glycol 8,000 18 w/v - Lithium sulfate 0.2 M - 🔍	NA	66 μl of Lithium sulfate (3 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 573 μl of water (100 v/v)
C2	Polyethylene glycol 8,000 18 w/v - 1,4-Dioxane 5 v/v - tris chloride BCC 0.1 M pH 8 Sodium fluoride 0.2 M - 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 250 μl of Sodium fluoride (0.8 M) 100 μl of 1,4-Dioxane (50 v/v) 360 μl of Polyethylene glycol 8,000 (50 w/v) 190 μl of water (100 v/v)
C3	Polyethylene glycol 8,000 25 w/v - Jeffamine® M-600 5 v/v - MES 0.09 M pH 6 Magnesium acetate 0.09 M - 🔍	pH 6	30 μl of MES pH 6 (3 M) 100 μl of Jeffamine® M-600 (50 v/v) 500 μl of Polyethylene glycol 8,000 (50 w/v) 45 μl of Magnesium acetate (2 M) 325 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 25 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium sulfate 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium sulfate (2 M) 500 μl of Polyethylene glycol 8,000 (50 w/v) 300 μl of water (100 v/v)
C5	Polyethylene glycol 10,000 5 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	100 μl of Polyethylene glycol 10,000 (50 w/v) 80 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 720 μl of water (100 v/v)
C6	Polyethylene glycol 10,000 10 w/v - Sodium acetate 0.05 M pH 5 🔍	pH 5	200 μl of Polyethylene glycol 10,000 (50 w/v) 16 μl of Sodium acetate pH 5 (3 M) 783 μl of water (100 v/v)
D1	Polyethylene glycol 10,000 10 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 700 μl of water (100 v/v)
D2	Polyethylene glycol 10,000 15 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 0.1 M - n-Dodecyl-N,N-dimethylamine-N-oxide 5 mM - 🔍	pH 5.5	50 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM) 300 μl of Polyethylene glycol 10,000 (50 w/v) 20 μl of Ammonium acetate (5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 530 μl of water (100 v/v)
D3	Polyethylene glycol 10,000 15 w/v - Glycine 0.06 M pH 9 tri-Sodium citrate 0.02 M - 🔍	pH 9	300 μl of Polyethylene glycol 10,000 (50 w/v) 30 μl of Glycine pH 9 (2 M) 10 μl of tri-Sodium citrate (2 M) 660 μl of water (100 v/v)
D4	Polyethylene glycol 10,000 20 w/v - Polyethylene glycol monomethyl ether 550 5 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	50 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 400 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 450 μl of water (100 v/v)
D5	Polyethylene glycol 20,000 12 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 240 μl of Polyethylene glycol 20,000 (50 w/v) 660 μl of water (100 v/v)
D6	Polyethylene glycol 20,000 17 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.1 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 340 μl of Polyethylene glycol 20,000 (50 w/v) 40 μl of Magnesium chloride pH 7 (2.5 M) 520 μl of water (100 v/v)