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Supplemental Information

Screen Description

Phosphatase 3

Phosphatase / Phosphatase 3
Created 2009-11-16.

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Phosphatase 3

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Screen Structure

Phosphatase 3

Well Count 24
Number of distinct ingredients 31
Internal diversity (score mean dist) 0.881

Screen Composition

2025-08-16T14:27:59.868248 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Phosphatase 3
Buffer 29
Cryoprotectant 1
Detergent 1
Organic (non-volatile) 2
Organic (volatile) 1
Polymer 26
Salt 25

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Phosphatase 3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 8,000 8 w/v -
  • Potassium di-hydrogen phosphate 0.1 M -
  • ß-Mercaptoethanol 0.2 v/v -
NA
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of ß-Mercaptoethanol (2 v/v)
690.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium acetate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 8,000 10 w/v -
  • MES 0.1 M pH 6.5
  • Potassium chloride 0.2 M -
  • Magnesium acetate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Potassium chloride (5 M)
610.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.05 M -
pH 8.5
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 8,000 12 w/v -
  • Sodium acetate 0.05 M pH 5
  • Ammonium acetate 0.3 M -
pH 5
60.0 μl of Ammonium acetate (5 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
16.7 μl of Sodium acetate pH 5 (3 M)
683.3 μl of water (100 v/v)
A6
  • Polyethylene glycol 8,000 12 w/v -
  • tris chloride BCC 0.1 M pH 6.3
  • Lithium sulfate 0.2 M -
pH 6.3
66.7 μl of Lithium sulfate (3 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of tris chloride BCC pH 6.3 (3 M)
660.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 8,000 12 w/v -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
580.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 8,000 13 w/v -
  • Glycerol 15 v/v -
  • MOPS 0.1 M pH 7
  • Ammonium sulfate 0.5 M -
pH 7
166.7 μl of Ammonium sulfate (3 M)
150.0 μl of Glycerol (100 v/v)
100.0 μl of MOPS pH 7 (1 M)
260.0 μl of Polyethylene glycol 8,000 (50 w/v)
323.3 μl of water (100 v/v)
B3
  • Polyethylene glycol 8,000 15 w/v -
  • Potassium di-hydrogen phosphate 0.1 M pH 4.6
pH 4.6
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M)
650.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 8,000 15 w/v -
  • Sodium di-hydrogen phosphate 0.1 M pH 4.6
pH 4.6
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M)
675.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 8,000 15 w/v -
  • di-Sodium succinate 0.1 M pH 5.5
  • Lithium sulfate 0.25 M -
pH 5.5
83.3 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of di-Sodium succinate pH 5.5 (3 M)
583.3 μl of water (100 v/v)
B6
  • Polyethylene glycol 8,000 18 w/v -
  • HEPES 0.1 M pH 7
  • Potassium di-hydrogen phosphate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
440.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 8,000 18 w/v -
  • Lithium sulfate 0.2 M -
NA
66.7 μl of Lithium sulfate (3 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
573.3 μl of water (100 v/v)
C2
  • Polyethylene glycol 8,000 18 w/v -
  • 1,4-Dioxane 5 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium fluoride 0.2 M -
pH 8
100.0 μl of 1,4-Dioxane (50 v/v)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
190.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 8,000 25 w/v -
  • Jeffamine® M-600 5 v/v -
  • MES 0.09 M pH 6
  • Magnesium acetate 0.09 M -
pH 6
100.0 μl of Jeffamine® M-600 (50 v/v)
30.0 μl of MES pH 6 (3 M)
45.0 μl of Magnesium acetate (2 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
325.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 8,000 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium sulfate 0.2 M -
pH 8.5
100.0 μl of Magnesium sulfate (2 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 10,000 5 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
100.0 μl of Polyethylene glycol 10,000 (50 w/v)
720.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 10,000 10 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
16.7 μl of Sodium acetate pH 5 (3 M)
783.3 μl of water (100 v/v)
D1
  • Polyethylene glycol 10,000 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
700.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 10,000 15 w/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium acetate 0.1 M -
  • n-Dodecyl-N,N-dimethylamine-N-oxide 5 mM -
pH 5.5
20.0 μl of Ammonium acetate (5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
50.0 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM)
530.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 10,000 15 w/v -
  • Glycine 0.06 M pH 9
  • tri-Sodium citrate 0.02 M -
pH 9
30.0 μl of Glycine pH 9 (2 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
10.0 μl of tri-Sodium citrate (2 M)
660.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 10,000 20 w/v -
  • Polyethylene glycol monomethyl ether 550 5 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
50.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
450.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 20,000 12 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 20,000 17 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.1 M -
pH 8.5
40.0 μl of Magnesium chloride pH 7 (2.5 M)
340.0 μl of Polyethylene glycol 20,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)