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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Phosphatase 3
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Phosphatase 3
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Screen Structure
Phosphatase 3
Well Count | 24 |
---|---|
Number of distinct ingredients | 31 |
Internal diversity (score mean dist) | 0.881 |
Screen Composition
ingredient type | Phosphatase 3 |
---|---|
Buffer | 29 |
Cryoprotectant | 1 |
Detergent | 1 |
Organic (non-volatile) | 2 |
Organic (volatile) | 1 |
Polymer | 26 |
Salt | 25 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 100.0 μl of ß-Mercaptoethanol (2 v/v) 690.0 μl of water (100 v/v) |
A2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
A3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Potassium chloride (5 M) 610.0 μl of water (100 v/v) |
A4 |
| pH 8.5 | 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 650.0 μl of water (100 v/v) |
A5 |
| pH 5 | 60.0 μl of Ammonium acetate (5 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 16.7 μl of Sodium acetate pH 5 (3 M) 683.3 μl of water (100 v/v) |
A6 |
| pH 6.3 | 66.7 μl of Lithium sulfate (3 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 33.3 μl of tris chloride BCC pH 6.3 (3 M) 660.0 μl of water (100 v/v) |
B1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 580.0 μl of water (100 v/v) |
B2 |
| pH 7 | 166.7 μl of Ammonium sulfate (3 M) 150.0 μl of Glycerol (100 v/v) 100.0 μl of MOPS pH 7 (1 M) 260.0 μl of Polyethylene glycol 8,000 (50 w/v) 323.3 μl of water (100 v/v) |
B3 |
| pH 4.6 | 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 650.0 μl of water (100 v/v) |
B4 |
| pH 4.6 | 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 25.0 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M) 675.0 μl of water (100 v/v) |
B5 |
| pH 5.5 | 83.3 μl of Lithium sulfate (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 33.3 μl of di-Sodium succinate pH 5.5 (3 M) 583.3 μl of water (100 v/v) |
B6 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Potassium di-hydrogen phosphate pH 7 (1 M) 440.0 μl of water (100 v/v) |
C1 |
| NA | 66.7 μl of Lithium sulfate (3 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 573.3 μl of water (100 v/v) |
C2 |
| pH 8 | 100.0 μl of 1,4-Dioxane (50 v/v) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 190.0 μl of water (100 v/v) |
C3 |
| pH 6 | 100.0 μl of Jeffamine® M-600 (50 v/v) 30.0 μl of MES pH 6 (3 M) 45.0 μl of Magnesium acetate (2 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 325.0 μl of water (100 v/v) |
C4 |
| pH 8.5 | 100.0 μl of Magnesium sulfate (2 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
C5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 100.0 μl of Polyethylene glycol 10,000 (50 w/v) 720.0 μl of water (100 v/v) |
C6 |
| pH 5 | 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 16.7 μl of Sodium acetate pH 5 (3 M) 783.3 μl of water (100 v/v) |
D1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 700.0 μl of water (100 v/v) |
D2 |
| pH 5.5 | 20.0 μl of Ammonium acetate (5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 50.0 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM) 530.0 μl of water (100 v/v) |
D3 |
| pH 9 | 30.0 μl of Glycine pH 9 (2 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 10.0 μl of tri-Sodium citrate (2 M) 660.0 μl of water (100 v/v) |
D4 |
| pH 5.6 | 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 50.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 450.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 40.0 μl of Magnesium chloride pH 7 (2.5 M) 340.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v) |