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Screen Description

Phosphatase 4

Phosphatase / Phosphatase 4
Created 2009-11-16.

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Phosphatase 4

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Screen Structure

Phosphatase 4

Well Count 24
Number of distinct ingredients 25
Internal diversity (score mean dist) 0.852

Screen Composition

2025-08-16T09:05:43.311619 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Phosphatase 4
Buffer 29
Cryoprotectant 1
Organic (non-volatile) 9
Polymer 4
Salt 36

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Phosphatase 4 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 0.5 M -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.2 M -
pH 7.5
166.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
66.7 μl of Lithium sulfate (3 M)
666.7 μl of water (100 v/v)
A2
  • Ammonium sulfate 1 M -
  • MES 0.1 M pH 6.5
  • Potassium chloride 0.1 M -
pH 6.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
20.0 μl of Potassium chloride (5 M)
546.7 μl of water (100 v/v)
A3
  • Ammonium sulfate 1 M -
  • Potassium chloride 0.8 M -
  • HEPES 0.1 M pH 7
pH 7
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7 (1 M)
160.0 μl of Potassium chloride (5 M)
406.7 μl of water (100 v/v)
A4
  • Ammonium sulfate 1.3 M -
  • CHES 0.1 M pH 9.5
  • Sodium chloride 0.2 M -
pH 9.5
433.3 μl of Ammonium sulfate (3 M)
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Sodium chloride (1 M)
266.7 μl of water (100 v/v)
A5
  • Ammonium sulfate 1.3 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
433.3 μl of Ammonium sulfate (3 M)
33.3 μl of Sodium acetate pH 5.5 (3 M)
533.3 μl of water (100 v/v)
A6
  • Ammonium sulfate 1.3 M -
  • Glycerol 5 v/v -
  • di-Sodium maleate 0.1 M pH 6.5
  • Magnesium chloride 0.001 M -
  • Zinc chloride 0.005 M -
pH 6.5
433.3 μl of Ammonium sulfate (3 M)
50.0 μl of Glycerol (100 v/v)
0.4 μl of Magnesium chloride pH 7 (2.5 M)
5.0 μl of Zinc chloride (1 M)
100.0 μl of di-Sodium maleate pH 6.5 (1 M)
411.3 μl of water (100 v/v)
B1
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
66.7 μl of Lithium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
B2
  • Ammonium sulfate 1.7 M -
  • MES 0.1 M pH 6
pH 6
566.7 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6 (3 M)
400.0 μl of water (100 v/v)
B3
  • Ammonium sulfate 1.7 M -
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
566.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
293.3 μl of water (100 v/v)
B4
  • Ammonium sulfate 1.7 M -
  • Polyethylene glycol 400 2 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
566.7 μl of Ammonium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of tris chloride BCC pH 7.5 (3 M)
380.0 μl of water (100 v/v)
B5
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B6
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 10 v/v -
  • HEPES 0.05 M pH 7.5
  • Zinc sulfate 0.05 M -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
50.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Zinc sulfate (1 M)
133.3 μl of water (100 v/v)
C1
  • Ammonium sulfate 2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Potassium Sodium tartrate 0.2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
133.3 μl of water (100 v/v)
C2
  • Ammonium sulfate 2.2 M -
  • Polypropylene glycol 400 6 v/v -
  • BIS-TRIS propane 0.1 M pH 6.5
pH 6.5
733.3 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
60.0 μl of Polypropylene glycol 400 (100 v/v)
106.7 μl of water (100 v/v)
C3
  • Ammonium sulfate 2.4 M -
  • HEPES 0.1 M pH 7
pH 7
800.0 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7 (1 M)
100.0 μl of water (100 v/v)
C4
  • Ammonium sulfate 2.4 M -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium chloride 0.01 M -
  • Zinc sulfate 0.005 M -
pH 8
800.0 μl of Ammonium sulfate (3 M)
10.0 μl of Magnesium chloride pH 7 (1 M)
5.0 μl of Zinc sulfate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
85.0 μl of water (100 v/v)
C5
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 400 10 v/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
166.7 μl of Lithium sulfate (3 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
633.3 μl of water (100 v/v)
C6
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Lithium sulfate (3 M)
400.0 μl of water (100 v/v)
D1
  • Lithium sulfate 2 M -
  • Polyethylene glycol 400 2 v/v -
  • TRIS-Acetate 0.1 M pH 8
  • ß-Mercaptoethanol 0.1 v/v -
pH 8
666.7 μl of Lithium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of TRIS-Acetate pH 8 (2 M)
50.0 μl of ß-Mercaptoethanol (2 v/v)
213.3 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • MES 0.1 M pH 6.5
pH 6.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
D3
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
D4
  • Sodium formate 1.4 M -
  • MES 0.1 M pH 6
pH 6
33.3 μl of MES pH 6 (3 M)
200.0 μl of Sodium formate (7 M)
766.7 μl of water (100 v/v)
D5
  • Sodium formate 3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
428.6 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
471.4 μl of water (100 v/v)
D6
  • di-Sodium tartrate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of di-Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)