C6 - Screen Contents - Phosphatase 4

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Supplemental Information

Screen Description

Phosphatase 4

Phosphatase / Phosphatase 4

Created 2009-11-16.

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Phosphatase 4

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Phosphatase 4

Screen Structure

Phosphatase 4

Well Count	24
Number of distinct ingredients	25
Internal diversity (score mean dist)	0.852

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase 4
Buffer	29
Cryoprotectant	1
Organic (non-volatile)	9
Polymer	4
Salt	36

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 Lithium sulfate 0.2 M - 🔍	pH 7.5	66 μl of Lithium sulfate (3 M) 100 μl of HEPES pH 7.5 (1 M) 166 μl of Ammonium sulfate (3 M) 666 μl of water (100 v/v)
A2	Ammonium sulfate 1 M - MES 0.1 M pH 6.5 Potassium chloride 0.1 M - 🔍	pH 6.5	20 μl of Potassium chloride (5 M) 333 μl of Ammonium sulfate (3 M) 100 μl of MES pH 6.5 (1 M) 546 μl of water (100 v/v)
A3	Ammonium sulfate 1 M - Potassium chloride 0.8 M - HEPES 0.1 M pH 7 🔍	pH 7	160 μl of Potassium chloride (5 M) 333 μl of Ammonium sulfate (3 M) 100 μl of HEPES pH 7 (1 M) 406 μl of water (100 v/v)
A4	Ammonium sulfate 1.3 M - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	433 μl of Ammonium sulfate (3 M) 100 μl of CHES pH 9.5 (1 M) 200 μl of Sodium chloride (1 M) 266 μl of water (100 v/v)
A5	Ammonium sulfate 1.3 M - Sodium acetate 0.1 M pH 5.5 🔍	pH 5.5	433 μl of Ammonium sulfate (3 M) 33 μl of Sodium acetate pH 5.5 (3 M) 533 μl of water (100 v/v)
A6	Ammonium sulfate 1.3 M - Glycerol 5 v/v - di-Sodium maleate 0.1 M pH 6.5 Magnesium chloride 0.001 M - Zinc chloride 0.005 M - 🔍	pH 6.5	433 μl of Ammonium sulfate (3 M) 50 μl of Glycerol (100 v/v) 0 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of di-Sodium maleate pH 6.5 (1 M) 5 μl of Zinc chloride (1 M) 411 μl of water (100 v/v)
B1	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	66 μl of Lithium sulfate (3 M) 500 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 333 μl of water (100 v/v)
B2	Ammonium sulfate 1.7 M - MES 0.1 M pH 6 🔍	pH 6	566 μl of Ammonium sulfate (3 M) 33 μl of MES pH 6 (3 M) 400 μl of water (100 v/v)
B3	Ammonium sulfate 1.7 M - 2-methyl-2,4-pentanediol BCC 4 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 566 μl of Ammonium sulfate (3 M) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 293 μl of water (100 v/v)
B4	Ammonium sulfate 1.7 M - Polyethylene glycol 400 2 v/v - tris chloride BCC 0.1 M pH 7.5 🔍	pH 7.5	20 μl of Polyethylene glycol 400 (100 v/v) 566 μl of Ammonium sulfate (3 M) 33 μl of tris chloride BCC pH 7.5 (3 M) 380 μl of water (100 v/v)
B5	Ammonium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	666 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)
B6	Ammonium sulfate 2 M - Polyethylene glycol 400 10 v/v - HEPES 0.05 M pH 7.5 Zinc sulfate 0.05 M - 🔍	pH 7.5	50 μl of Zinc sulfate (1 M) 100 μl of Polyethylene glycol 400 (100 v/v) 50 μl of HEPES pH 7.5 (1 M) 666 μl of Ammonium sulfate (3 M) 133 μl of water (100 v/v)
C1	Ammonium sulfate 2 M - tri-Sodium citrate 0.1 M pH 5.6 Potassium Sodium tartrate 0.2 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 100 μl of Potassium Sodium tartrate (2 M) 666 μl of Ammonium sulfate (3 M) 133 μl of water (100 v/v)
C2	Ammonium sulfate 2.2 M - Polypropylene glycol 400 6 v/v - BIS-TRIS propane 0.1 M pH 6.5 🔍	pH 6.5	60 μl of Polypropylene glycol 400 (100 v/v) 733 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 106 μl of water (100 v/v)
C3	Ammonium sulfate 2.4 M - HEPES 0.1 M pH 7 🔍	pH 7	800 μl of Ammonium sulfate (3 M) 100 μl of HEPES pH 7 (1 M) 100 μl of water (100 v/v)
C4	Ammonium sulfate 2.4 M - tris chloride BCC 0.1 M pH 8 Magnesium chloride 0.01 M - Zinc sulfate 0.005 M - 🔍	pH 8	5 μl of Zinc sulfate (1 M) 800 μl of Ammonium sulfate (3 M) 10 μl of Magnesium chloride pH 7 (1 M) 100 μl of tris chloride BCC pH 8 (1 M) 85 μl of water (100 v/v)
C5	Lithium sulfate 0.5 M - Polyethylene glycol 400 10 v/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Polyethylene glycol 400 (100 v/v) 166 μl of Lithium sulfate (3 M) 100 μl of Imidazole pH 8 (1 M) 633 μl of water (100 v/v)
C6	Lithium sulfate 1.5 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	500 μl of Lithium sulfate (3 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v)
D1	Lithium sulfate 2 M - Polyethylene glycol 400 2 v/v - TRIS-Acetate 0.1 M pH 8 ß-Mercaptoethanol 0.1 v/v - 🔍	pH 8	20 μl of Polyethylene glycol 400 (100 v/v) 666 μl of Lithium sulfate (3 M) 50 μl of TRIS-Acetate pH 8 (2 M) 50 μl of ß-Mercaptoethanol (2 v/v) 213 μl of water (100 v/v)
D2	2-methyl-2,4-pentanediol BCC 20 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 700 μl of water (100 v/v)
D3	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 550 μl of water (100 v/v)
D4	Sodium formate 1.4 M - MES 0.1 M pH 6 🔍	pH 6	200 μl of Sodium formate (7 M) 33 μl of MES pH 6 (3 M) 766 μl of water (100 v/v)
D5	Sodium formate 3 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	428 μl of Sodium formate (7 M) 100 μl of tris chloride BCC pH 8 (1 M) 471 μl of water (100 v/v)
D6	di-Sodium tartrate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of di-Sodium tartrate (2 M) 400 μl of water (100 v/v)