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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Phosphatase 4
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Phosphatase 4
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Screen Structure
Phosphatase 4
Well Count | 24 |
---|---|
Number of distinct ingredients | 25 |
Internal diversity (score mean dist) | 0.852 |
Screen Composition
ingredient type | Phosphatase 4 |
---|---|
Buffer | 29 |
Cryoprotectant | 1 |
Organic (non-volatile) | 9 |
Polymer | 4 |
Salt | 36 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7.5 | 166.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 66.7 μl of Lithium sulfate (3 M) 666.7 μl of water (100 v/v) |
A2 |
| pH 6.5 | 333.3 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 20.0 μl of Potassium chloride (5 M) 546.7 μl of water (100 v/v) |
A3 |
| pH 7 | 333.3 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7 (1 M) 160.0 μl of Potassium chloride (5 M) 406.7 μl of water (100 v/v) |
A4 |
| pH 9.5 | 433.3 μl of Ammonium sulfate (3 M) 100.0 μl of CHES pH 9.5 (1 M) 200.0 μl of Sodium chloride (1 M) 266.7 μl of water (100 v/v) |
A5 |
| pH 5.5 | 433.3 μl of Ammonium sulfate (3 M) 33.3 μl of Sodium acetate pH 5.5 (3 M) 533.3 μl of water (100 v/v) |
A6 |
| pH 6.5 | 433.3 μl of Ammonium sulfate (3 M) 50.0 μl of Glycerol (100 v/v) 0.4 μl of Magnesium chloride pH 7 (2.5 M) 5.0 μl of Zinc chloride (1 M) 100.0 μl of di-Sodium maleate pH 6.5 (1 M) 411.3 μl of water (100 v/v) |
B1 |
| pH 8.5 | 500.0 μl of Ammonium sulfate (3 M) 66.7 μl of Lithium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v) |
B2 |
| pH 6 | 566.7 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6 (3 M) 400.0 μl of water (100 v/v) |
B3 |
| pH 7.5 | 40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 566.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 293.3 μl of water (100 v/v) |
B4 |
| pH 7.5 | 566.7 μl of Ammonium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of tris chloride BCC pH 7.5 (3 M) 380.0 μl of water (100 v/v) |
B5 |
| pH 8.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
B6 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 50.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Zinc sulfate (1 M) 133.3 μl of water (100 v/v) |
C1 |
| pH 5.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Potassium Sodium tartrate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 133.3 μl of water (100 v/v) |
C2 |
| pH 6.5 | 733.3 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 60.0 μl of Polypropylene glycol 400 (100 v/v) 106.7 μl of water (100 v/v) |
C3 |
| pH 7 | 800.0 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7 (1 M) 100.0 μl of water (100 v/v) |
C4 |
| pH 8 | 800.0 μl of Ammonium sulfate (3 M) 10.0 μl of Magnesium chloride pH 7 (1 M) 5.0 μl of Zinc sulfate (1 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 85.0 μl of water (100 v/v) |
C5 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 166.7 μl of Lithium sulfate (3 M) 100.0 μl of Polyethylene glycol 400 (100 v/v) 633.3 μl of water (100 v/v) |
C6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Lithium sulfate (3 M) 400.0 μl of water (100 v/v) |
D1 |
| pH 8 | 666.7 μl of Lithium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of TRIS-Acetate pH 8 (2 M) 50.0 μl of ß-Mercaptoethanol (2 v/v) 213.3 μl of water (100 v/v) |
D2 |
| pH 6.5 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 700.0 μl of water (100 v/v) |
D3 |
| pH 4.6 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of water (100 v/v) |
D4 |
| pH 6 | 33.3 μl of MES pH 6 (3 M) 200.0 μl of Sodium formate (7 M) 766.7 μl of water (100 v/v) |
D5 |
| pH 8 | 428.6 μl of Sodium formate (7 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 471.4 μl of water (100 v/v) |
D6 |
| pH 8.5 | 500.0 μl of di-Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |