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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG Rx HT
Barcodes
PEG Rx HT
Screen Structure
PEG Rx HT
Well Count | 96 |
---|---|
Number of distinct ingredients | 49 |
Internal diversity (score mean dist) | 0.951 |
Screen Composition
ingredient type | PEG Rx HT |
---|---|
Buffer | 111 |
Complex ingredient | 4 |
Detergent | 1 |
Organic (non-volatile) | 14 |
Organic (volatile) | 13 |
Polymer | 98 |
Salt | 69 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 340 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 560 μl of water (100 v/v) |
A2 |
| pH 5.5 | 380 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 520 μl of water (100 v/v) |
A3 |
| pH 7.5 | 420 μl of Polyethylene glycol 200 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 480 μl of water (100 v/v) |
A4 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 300 μl of Polyethylene glycol 300 (100 v/v) 600 μl of water (100 v/v) |
A5 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 250 μl of Polyethylene glycol 300 (100 v/v) 650 μl of water (100 v/v) |
A6 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 200 μl of Polyethylene glycol 300 (100 v/v) 700 μl of water (100 v/v) |
A7 |
| pH 4 | 100 μl of Sodium acetate pH 4 (1 M) 150 μl of Polyethylene glycol 400 (100 v/v) 750 μl of water (100 v/v) |
A8 |
| pH 6 | 100 μl of MES pH 6 (1 M) 220 μl of Polyethylene glycol 400 (100 v/v) 680 μl of water (100 v/v) |
A9 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 600 μl of water (100 v/v) |
A10 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 600 μl of water (100 v/v) |
A11 |
| pH 7 | 250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Imidazole pH 7 (1 M) 650 μl of water (100 v/v) |
A12 |
| pH 9 | 200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 700 μl of water (100 v/v) |
B1 |
| pH 4 | 100 μl of Sodium acetate pH 4 (1 M) 200 μl of Jeffamine® M-600 pH 7 (50 v/v) 700 μl of water (100 v/v) |
B2 |
| pH 6 | 100 μl of MES pH 6 (1 M) 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 500 μl of water (100 v/v) |
B3 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 300 μl of water (100 v/v) |
B4 |
| pH 3.5 | 280 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 620 μl of water (100 v/v) |
B5 |
| pH 5.5 | 440 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 460 μl of water (100 v/v) |
B6 |
| pH 7.5 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |
B7 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 600 μl of Polyethylene glycol 1,500 (50 w/v) 300 μl of water (100 v/v) |
B8 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 400 μl of Polyethylene glycol 1,500 (50 w/v) 500 μl of water (100 v/v) |
B9 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 300 μl of Polyethylene glycol 1,500 (50 w/v) 600 μl of water (100 v/v) |
B10 |
| pH 4 | 200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Sodium acetate pH 4 (1 M) 700 μl of water (100 v/v) |
B11 |
| pH 6 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 500 μl of water (100 v/v) |
B12 |
| pH 8 | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of water (100 v/v) |
C1 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 600 μl of Jeffamine® ED-2001 (50 w/v) 300 μl of water (100 v/v) |
C2 |
| pH 7 | 100 μl of Imidazole pH 7 (1 M) 400 μl of Jeffamine® ED-2001 (50 w/v) 500 μl of water (100 v/v) |
C3 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 200 μl of Jeffamine® ED-2001 (50 w/v) 700 μl of water (100 v/v) |
C4 |
| pH 3.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 400 μl of water (100 v/v) |
C5 |
| pH 5.5 | 360 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 540 μl of water (100 v/v) |
C6 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 240 μl of Polyethylene glycol 3,350 (50 w/v) 660 μl of water (100 v/v) |
C7 |
| pH 4 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4 (1 M) 700 μl of water (100 v/v) |
C8 |
| pH 6 | 280 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 620 μl of water (100 v/v) |
C9 |
| pH 8 | 560 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 340 μl of water (100 v/v) |
C10 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 300 μl of water (100 v/v) |
C11 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500 μl of water (100 v/v) |
C12 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 160 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 740 μl of water (100 v/v) |
D1 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
D2 |
| pH 7 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 500 μl of water (100 v/v) |
D3 |
| pH 9 | 600 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 300 μl of water (100 v/v) |
D4 |
| pH 3.5 | 560 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 340 μl of water (100 v/v) |
D5 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 580 μl of water (100 v/v) |
D6 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 80 μl of Polyethylene glycol 8,000 (50 w/v) 820 μl of water (100 v/v) |
D7 |
| pH 4.5 | 200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of Sodium acetate pH 4.5 (1 M) 700 μl of water (100 v/v) |
D8 |
| pH 6.5 | 320 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 580 μl of water (100 v/v) |
D9 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 500 μl of water (100 v/v) |
D10 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 600 μl of Polyethylene glycol 20,000 (30 w/v) 300 μl of water (100 v/v) |
D11 |
| pH 7 | 400 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of Imidazole pH 7 (1 M) 500 μl of water (100 v/v) |
D12 |
| pH 9 | 266 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 633 μl of water (100 v/v) |
E1 |
| pH 4 | 40 μl of Polyethylene glycol 200 (100 v/v) 400 μl of Lithium sulfate (2 M) 100 μl of Sodium acetate pH 4 (1 M) 460 μl of water (100 v/v) |
E2 |
| pH 5 | 260 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 100 μl of Lithium sulfate (2 M) 540 μl of water (100 v/v) |
E3 |
| pH 6 | 450 μl of Polyethylene glycol 200 (100 v/v) 25 μl of Calcium chloride (2 M) 100 μl of MES pH 6 (1 M) 425 μl of water (100 v/v) |
E4 |
| pH 6.5 | 30 μl of Polyethylene glycol 200 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 280 μl of 2-Propanol (100 v/v) 590 μl of water (100 v/v) |
E5 |
| NA | 20 μl of Polyethylene glycol 200 (100 v/v) 200 μl of Tacsimate pH 7 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 680 μl of water (100 v/v) |
E6 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 260 μl of Polyethylene glycol 400 (100 v/v) 100 μl of 2-Propanol (100 v/v) 540 μl of water (100 v/v) |
E7 |
| pH 5.5 | 200 μl of Ammonium acetate (1 M) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 240 μl of Polyethylene glycol 400 (100 v/v) 460 μl of water (100 v/v) |
E8 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 180 μl of Polyethylene glycol 400 (100 v/v) 57 μl of Ammonium sulfate (3.5 M) 662 μl of water (100 v/v) |
E9 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 500 μl of water (100 v/v) |
E10 |
| pH 4.5 | 260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Sodium acetate pH 4.5 (1 M) 60 μl of 2-Propanol (100 v/v) 580 μl of water (100 v/v) |
E11 |
| pH 6.5 | 20 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 514 μl of Ammonium sulfate (3.5 M) 365 μl of water (100 v/v) |
E12 |
| pH 7 | 220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Imidazole pH 7 (1 M) 50 μl of di-Sodium DL-malate (3 M) 630 μl of water (100 v/v) |
F1 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 83 μl of di-Sodium succinate (1.2 M) 516 μl of water (100 v/v) |
F2 |
| pH 5.5 | 400 μl of Polyethylene glycol 1,000 (50 w/v) 50 μl of Lithium sulfate (2 M) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 450 μl of water (100 v/v) |
F3 |
| pH 8 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 29 μl of di-Sodium malonate pH 8 (3.4 M) 270 μl of water (100 v/v) |
F4 |
| pH 3.5 | 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 460 μl of water (100 v/v) |
F5 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 200 μl of L-Proline (1 M) 480 μl of Polyethylene glycol 1,500 (50 w/v) 220 μl of water (100 v/v) |
F6 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 600 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of 2-Propanol (100 v/v) 200 μl of water (100 v/v) |
F7 |
| pH 9 | 20 μl of Sodium chloride (5 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 380 μl of water (100 v/v) |
F8 |
| pH 4.5 | 480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 10 μl of Magnesium chloride (2 M) 100 μl of Sodium acetate pH 4.5 (1 M) 5 μl of Nickel (II) chloride (4 M) 20 μl of Cadmium chloride (1 M) 385 μl of water (100 v/v) |
F9 |
| pH 6 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 200 μl of 2-Propanol (100 v/v) 300 μl of water (100 v/v) |
F10 |
| pH 7 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 80 μl of tri-Ammonium citrate (2.5 M) 100 μl of Imidazole pH 7 (1 M) 420 μl of water (100 v/v) |
F11 |
| pH 9 | 40 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 285 μl of Potassium formate (14 M) 100 μl of BIS-TRIS propane pH 9 (1 M) 574 μl of water (100 v/v) |
F12 |
| NA | 100 μl of Sodium acetate pH 4.5 (1 M) 20 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of Tacsimate pH 4 (100 v/v) 380 μl of water (100 v/v) |
G1 |
| pH 5.5 | 20 μl of n-Octyl-ß-D-glucoside (5 w/v) 440 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 440 μl of water (100 v/v) |
G2 |
| NA | 20 μl of Tacsimate pH 7 (100 v/v) 160 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 50 μl of 2-Propanol (100 v/v) 670 μl of water (100 v/v) |
G3 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of 1,4-Dioxane (100 v/v) 580 μl of water (100 v/v) |
G4 |
| pH 5.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 180 μl of 2-Propanol (100 v/v) 320 μl of water (100 v/v) |
G5 |
| NA | 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 60 μl of Tacsimate pH 6 (100 v/v) 340 μl of water (100 v/v) |
G6 |
| pH 4 | 200 μl of Magnesium formate (1 M) 100 μl of Sodium acetate pH 4 (1 M) 360 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 340 μl of water (100 v/v) |
G7 |
| pH 7 | 100 μl of Imidazole pH 7 (1 M) 480 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 20 μl of Polyethylene glycol 400 (100 v/v) 400 μl of water (100 v/v) |
G8 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 28 μl of Sodium formate (7 M) 471 μl of water (100 v/v) |
G9 |
| pH 9 | 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 40 μl of 2-Propanol (100 v/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 460 μl of water (100 v/v) |
G10 |
| pH 3.5 | 60 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 640 μl of water (100 v/v) |
G11 |
| pH 3.5 | 360 μl of Polyethylene glycol 6,000 (50 w/v) 75 μl of Lithium sulfate (2 M) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 465 μl of water (100 v/v) |
G12 |
| pH 4 | 440 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Sodium acetate pH 4 (1 M) 100 μl of 2-Propanol (100 v/v) 360 μl of water (100 v/v) |
H1 |
| pH 4 | 40 μl of Sodium chloride (5 M) 100 μl of Sodium acetate pH 4 (1 M) 440 μl of Polyethylene glycol 8,000 (50 w/v) 420 μl of water (100 v/v) |
H2 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 100 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v) |
H3 |
| pH 9 | 100 μl of Polyethylene glycol 200 (100 v/v) 360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 440 μl of water (100 v/v) |
H4 |
| pH 5 | 200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 150 μl of 2-Propanol (100 v/v) 550 μl of water (100 v/v) |
H5 |
| pH 6 | 117 μl of di-Sodium malonate pH 6 (3.4 M) 10 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of MES pH 6 (1 M) 772 μl of water (100 v/v) |
H6 |
| pH 6.5 | 133 μl of Potassium Sodium tartrate (1.5 M) 200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 566 μl of water (100 v/v) |
H7 |
| pH 7.5 | 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 650 μl of water (100 v/v) |
H8 |
| pH 8 | 320 μl of Polyethylene glycol 10,000 (50 w/v) 200 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8 (1 M) 380 μl of water (100 v/v) |
H9 |
| pH 3.5 | 200 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 50 μl of 2-Propanol (100 v/v) 650 μl of water (100 v/v) |
H10 |
| pH 4.5 | 66 μl of Polyethylene glycol 20,000 (30 w/v) 294 μl of di-Sodium malonate pH 5 (3.4 M) 100 μl of Sodium acetate pH 4.5 (1 M) 539 μl of water (100 v/v) |
H11 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of Magnesium chloride (2 M) 466 μl of water (100 v/v) |
H12 |
| pH 8.5 | 100 μl of BICINE pH 8.5 (1 M) 100 μl of Dextran sulfate sodium salt 5,000 (30 w/v) 500 μl of Polyethylene glycol 20,000 (30 w/v) 300 μl of water (100 v/v) |