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Supplemental Information

Screen Description

PEG Rx HT

Hampton Research / PEG Rx HT
Created 2009-11-16.

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PEG Rx HT

    Screen Structure

    PEG Rx HT

    Well Count 96
    Number of distinct ingredients 49
    Internal diversity (score mean dist) 0.951

    Screen Composition

    2025-06-14T13:43:10.266217 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Rx HT
    Buffer 111
    Complex ingredient 4
    Detergent 1
    Organic (non-volatile) 14
    Organic (volatile) 13
    Polymer 98
    Salt 69

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Rx HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 200 34 v/v -
    pH 3.5
    340 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    560 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 200 38 v/v -
    pH 5.5
    380 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    520 μl of water (100 v/v)
    A3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 42 v/v -
    pH 7.5
    420 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 30 v/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    600 μl of water (100 v/v)
    A5
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 300 25 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    250 μl of Polyethylene glycol 300 (100 v/v)
    650 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 300 20 v/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    700 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 400 15 v/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    750 μl of water (100 v/v)
    A8
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 22 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    680 μl of water (100 v/v)
    A9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    A10
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    600 μl of water (100 v/v)
    A11
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    pH 7
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Imidazole pH 7 (1 M)
    650 μl of water (100 v/v)
    A12
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 9
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    700 μl of water (100 v/v)
    B1
    • Sodium acetate 0.1 M pH 4
    • Jeffamine® M-600 10 v/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    200 μl of Jeffamine® M-600 pH 7 (50 v/v)
    700 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6
    • Jeffamine® M-600 20 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    400 μl of Jeffamine® M-600 pH 7 (50 v/v)
    500 μl of water (100 v/v)
    B3
    • tris chloride BCC 0.1 M pH 8
    • Jeffamine® M-600 30 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    300 μl of water (100 v/v)
    B4
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 1,000 14 w/v -
    pH 3.5
    280 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    620 μl of water (100 v/v)
    B5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 22 w/v -
    pH 5.5
    440 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    460 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 30 w/v -
    pH 7.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    B7
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,500 30 w/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of water (100 v/v)
    B8
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,500 20 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    500 μl of water (100 v/v)
    B9
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 1,500 15 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    600 μl of water (100 v/v)
    B10
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 4
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    700 μl of water (100 v/v)
    B11
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    500 μl of water (100 v/v)
    B12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 8
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 5
    • Jeffamine® ED-2001 30 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Jeffamine® ED-2001 (50 w/v)
    300 μl of water (100 v/v)
    C2
    • Imidazole 0.1 M pH 7
    • Jeffamine® ED-2001 20 w/v -
    pH 7
    100 μl of Imidazole pH 7 (1 M)
    400 μl of Jeffamine® ED-2001 (50 w/v)
    500 μl of water (100 v/v)
    C3
    • BIS-TRIS propane 0.1 M pH 9
    • Jeffamine® ED-2001 10 w/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    200 μl of Jeffamine® ED-2001 (50 w/v)
    700 μl of water (100 v/v)
    C4
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 3.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    400 μl of water (100 v/v)
    C5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 18 w/v -
    pH 5.5
    360 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    540 μl of water (100 v/v)
    C6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 12 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    660 μl of water (100 v/v)
    C7
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 4,000 10 w/v -
    pH 4
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    700 μl of water (100 v/v)
    C8
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 14 w/v -
    pH 6
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    620 μl of water (100 v/v)
    C9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 28 w/v -
    pH 8
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    340 μl of water (100 v/v)
    C10
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    300 μl of water (100 v/v)
    C11
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    500 μl of water (100 v/v)
    C12
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 8 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    160 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    740 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    D2
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    500 μl of water (100 v/v)
    D3
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 6,000 30 w/v -
    pH 9
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    300 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 8,000 28 w/v -
    pH 3.5
    560 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    340 μl of water (100 v/v)
    D5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 16 w/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    580 μl of water (100 v/v)
    D6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 4 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 8,000 (50 w/v)
    820 μl of water (100 v/v)
    D7
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 4.5
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    700 μl of water (100 v/v)
    D8
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 10,000 16 w/v -
    pH 6.5
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    580 μl of water (100 v/v)
    D9
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 10,000 20 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    D10
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 20,000 18 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Polyethylene glycol 20,000 (30 w/v)
    300 μl of water (100 v/v)
    D11
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 20,000 12 w/v -
    pH 7
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Imidazole pH 7 (1 M)
    500 μl of water (100 v/v)
    D12
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 20,000 8 w/v -
    pH 9
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    633 μl of water (100 v/v)
    E1
    • Lithium sulfate 0.8 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 200 4 v/v -
    pH 4
    40 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of Lithium sulfate (2 M)
    100 μl of Sodium acetate pH 4 (1 M)
    460 μl of water (100 v/v)
    E2
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 26 v/v -
    pH 5
    260 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Lithium sulfate (2 M)
    540 μl of water (100 v/v)
    E3
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 45 v/v -
    pH 6
    450 μl of Polyethylene glycol 200 (100 v/v)
    25 μl of Calcium chloride (2 M)
    100 μl of MES pH 6 (1 M)
    425 μl of water (100 v/v)
    E4
    • 2-Propanol 28 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 200 3 v/v -
    pH 6.5
    30 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    280 μl of 2-Propanol (100 v/v)
    590 μl of water (100 v/v)
    E5
    • Tacsimate 20 v/v pH 7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 2 v/v -
    NA
    20 μl of Polyethylene glycol 200 (100 v/v)
    200 μl of Tacsimate pH 7 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    680 μl of water (100 v/v)
    E6
    • 2-Propanol 10 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 26 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of 2-Propanol (100 v/v)
    540 μl of water (100 v/v)
    E7
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 24 v/v -
    pH 5.5
    200 μl of Ammonium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    460 μl of water (100 v/v)
    E8
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 18 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    180 μl of Polyethylene glycol 400 (100 v/v)
    57 μl of Ammonium sulfate (3.5 M)
    662 μl of water (100 v/v)
    E9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 40 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    E10
    • 2-Propanol 6 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 4.5
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    60 μl of 2-Propanol (100 v/v)
    580 μl of water (100 v/v)
    E11
    • Ammonium sulfate 1.8 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 2 v/v -
    pH 6.5
    20 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    365 μl of water (100 v/v)
    E12
    • di-Sodium DL-malate 0.15 M -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 7
    220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Imidazole pH 7 (1 M)
    50 μl of di-Sodium DL-malate (3 M)
    630 μl of water (100 v/v)
    F1
    • di-Sodium succinate 0.1 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    83 μl of di-Sodium succinate (1.2 M)
    516 μl of water (100 v/v)
    F2
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 20 w/v -
    pH 5.5
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    450 μl of water (100 v/v)
    F3
    • di-Sodium malonate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 1,000 30 w/v -
    pH 8
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    29 μl of di-Sodium malonate pH 8 (3.4 M)
    270 μl of water (100 v/v)
    F4
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 1,500 20 w/v -
    pH 3.5
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    460 μl of water (100 v/v)
    F5
    • L-Proline 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,500 24 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of L-Proline (1 M)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    220 μl of water (100 v/v)
    F6
    • 2-Propanol 10 v/v -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 1,500 30 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    200 μl of water (100 v/v)
    F7
    • Sodium chloride 0.1 M -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 1,500 25 w/v -
    pH 9
    20 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    380 μl of water (100 v/v)
    F8
    • Nickel (II) chloride 0.02 M -
    • Magnesium chloride 0.02 M -
    • Cadmium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 2,000 24 w/v -
    pH 4.5
    480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    10 μl of Magnesium chloride (2 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    5 μl of Nickel (II) chloride (4 M)
    20 μl of Cadmium chloride (1 M)
    385 μl of water (100 v/v)
    F9
    • 2-Propanol 20 v/v -
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    F10
    • tri-Ammonium citrate 0.2 M -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    80 μl of tri-Ammonium citrate (2.5 M)
    100 μl of Imidazole pH 7 (1 M)
    420 μl of water (100 v/v)
    F11
    • Potassium formate 4 M -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 2,000 2 w/v -
    pH 9
    40 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    285 μl of Potassium formate (14 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    574 μl of water (100 v/v)
    F12
    • Tacsimate 50 v/v pH 4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 1 w/v -
    NA
    100 μl of Sodium acetate pH 4.5 (1 M)
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of Tacsimate pH 4 (100 v/v)
    380 μl of water (100 v/v)
    G1
    • n-Octyl-ß-D-glucoside 0.1 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 22 w/v -
    pH 5.5
    20 μl of n-Octyl-ß-D-glucoside (5 w/v)
    440 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    440 μl of water (100 v/v)
    G2
    • Tacsimate 2 v/v pH 7
    • 2-Propanol 5 v/v -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 3,350 8 w/v -
    NA
    20 μl of Tacsimate pH 7 (100 v/v)
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    50 μl of 2-Propanol (100 v/v)
    670 μl of water (100 v/v)
    G3
    • 1,4-Dioxane 2 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 3,350 15 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of 1,4-Dioxane (100 v/v)
    580 μl of water (100 v/v)
    G4
    • 2-Propanol 18 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    180 μl of 2-Propanol (100 v/v)
    320 μl of water (100 v/v)
    G5
    • Tacsimate 6 v/v pH 6
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 25 w/v -
    NA
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    60 μl of Tacsimate pH 6 (100 v/v)
    340 μl of water (100 v/v)
    G6
    • Magnesium formate 0.2 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 5,000 18 w/v -
    pH 4
    200 μl of Magnesium formate (1 M)
    100 μl of Sodium acetate pH 4 (1 M)
    360 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    340 μl of water (100 v/v)
    G7
    • Polyethylene glycol 400 2 v/v -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 5,000 24 w/v -
    pH 7
    100 μl of Imidazole pH 7 (1 M)
    480 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    20 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    G8
    • Sodium formate 0.2 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    28 μl of Sodium formate (7 M)
    471 μl of water (100 v/v)
    G9
    • 2-Propanol 4 v/v -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 9
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    40 μl of 2-Propanol (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    460 μl of water (100 v/v)
    G10
    • Ethylene glycol 6 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 6,000 10 w/v -
    pH 3.5
    60 μl of Ethylene glycol (100 v/v)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    640 μl of water (100 v/v)
    G11
    • Lithium sulfate 0.15 M -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 6,000 18 w/v -
    pH 3.5
    360 μl of Polyethylene glycol 6,000 (50 w/v)
    75 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    465 μl of water (100 v/v)
    G12
    • 2-Propanol 10 v/v -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 6,000 22 w/v -
    pH 4
    440 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    100 μl of 2-Propanol (100 v/v)
    360 μl of water (100 v/v)
    H1
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 8,000 22 w/v -
    pH 4
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4 (1 M)
    440 μl of Polyethylene glycol 8,000 (50 w/v)
    420 μl of water (100 v/v)
    H2
    • 2-Propanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 5 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    H3
    • Polyethylene glycol 200 10 v/v -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 8,000 18 w/v -
    pH 9
    100 μl of Polyethylene glycol 200 (100 v/v)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    440 μl of water (100 v/v)
    H4
    • 2-Propanol 15 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 10,000 10 w/v -
    pH 5
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    150 μl of 2-Propanol (100 v/v)
    550 μl of water (100 v/v)
    H5
    • di-Sodium malonate 0.4 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 10,000 0.5 w/v -
    pH 6
    117 μl of di-Sodium malonate pH 6 (3.4 M)
    10 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    772 μl of water (100 v/v)
    H6
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 6.5
    133 μl of Potassium Sodium tartrate (1.5 M)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    566 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 7.5
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    H8
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 10,000 16 w/v -
    pH 8
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    380 μl of water (100 v/v)
    H9
    • 2-Propanol 5 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 20,000 6 w/v -
    pH 3.5
    200 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    650 μl of water (100 v/v)
    H10
    • di-Sodium malonate 1 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 20,000 2 w/v -
    pH 4.5
    66 μl of Polyethylene glycol 20,000 (30 w/v)
    294 μl of di-Sodium malonate pH 5 (3.4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    539 μl of water (100 v/v)
    H11
    • Magnesium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 20,000 10 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Magnesium chloride (2 M)
    466 μl of water (100 v/v)
    H12
    • Dextran sulfate sodium salt 5,000 3 w/v -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 20,000 15 w/v -
    pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    100 μl of Dextran sulfate sodium salt 5,000 (30 w/v)
    500 μl of Polyethylene glycol 20,000 (30 w/v)
    300 μl of water (100 v/v)