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Supplemental Information

Screen Description

PEG Rx 1

Hampton Research / PEG Rx 1
Created 2009-11-16.

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PEG Rx 1

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Screen Structure

PEG Rx 1

Well Count 48
Number of distinct ingredients 25
Internal diversity (score mean dist) 0.948

Screen Composition

2025-08-12T11:04:36.065754 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PEG Rx 1
Buffer 48
Organic (non-volatile) 6
Polymer 48
Salt 20

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG Rx 1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 200 34 v/v -
pH 3.5
340.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
560.0 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 200 38 v/v -
pH 5.5
380.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
520.0 μl of water (100 v/v)
A3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 200 42 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
420.0 μl of Polyethylene glycol 200 (100 v/v)
480.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 300 30 v/v -
pH 4.5
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
600.0 μl of water (100 v/v)
A5
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 300 25 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
250.0 μl of Polyethylene glycol 300 (100 v/v)
650.0 μl of water (100 v/v)
A6
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 300 20 v/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
200.0 μl of Polyethylene glycol 300 (100 v/v)
700.0 μl of water (100 v/v)
A7
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 400 15 v/v -
pH 4
150.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
750.0 μl of water (100 v/v)
A8
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 22 v/v -
pH 6
100.0 μl of MES pH 6 (1 M)
220.0 μl of Polyethylene glycol 400 (100 v/v)
680.0 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
pH 8
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
600.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 5
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
600.0 μl of water (100 v/v)
B3
  • Imidazole 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 7
100.0 μl of Imidazole pH 7 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
650.0 μl of water (100 v/v)
B4
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
700.0 μl of water (100 v/v)
B5
  • Sodium acetate 0.1 M pH 4
  • Jeffamine® M-600 10 v/v -
pH 4
200.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
B6
  • MES 0.1 M pH 6
  • Jeffamine® M-600 20 v/v -
pH 6
400.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of MES pH 6 (1 M)
500.0 μl of water (100 v/v)
B7
  • tris chloride BCC 0.1 M pH 8
  • Jeffamine® M-600 30 v/v -
pH 8
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 1,000 14 w/v -
pH 3.5
280.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
620.0 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 22 w/v -
pH 5.5
440.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
460.0 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 30 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
300.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,500 30 w/v -
pH 4.5
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
C4
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 1,500 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
C5
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 1,500 15 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
600.0 μl of water (100 v/v)
C6
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 4
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
C7
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
C8
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 8
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M pH 5
  • Jeffamine® ED-2001 30 w/v -
pH 5
600.0 μl of Jeffamine® ED-2001 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
D2
  • Imidazole 0.1 M pH 7
  • Jeffamine® ED-2001 20 w/v -
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Jeffamine® ED-2001 (50 w/v)
500.0 μl of water (100 v/v)
D3
  • BIS-TRIS propane 0.1 M pH 9
  • Jeffamine® ED-2001 10 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
200.0 μl of Jeffamine® ED-2001 (50 w/v)
700.0 μl of water (100 v/v)
D4
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 3,350 25 w/v -
pH 3.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 18 w/v -
pH 5.5
360.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
540.0 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
660.0 μl of water (100 v/v)
D7
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 4,000 10 w/v -
pH 4
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
D8
  • MES 0.1 M pH 6
  • Polyethylene glycol 4,000 14 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
620.0 μl of water (100 v/v)
E1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 28 w/v -
pH 8
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
E2
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 4.5
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
E3
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
E4
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 8 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
740.0 μl of water (100 v/v)
E5
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
E6
  • Imidazole 0.1 M pH 7
  • Polyethylene glycol 6,000 20 w/v -
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
E7
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 6,000 30 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
E8
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 8,000 28 w/v -
pH 3.5
560.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
340.0 μl of water (100 v/v)
F1
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 16 w/v -
pH 5.5
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
F2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 4 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol 8,000 (50 w/v)
820.0 μl of water (100 v/v)
F3
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 10 w/v -
pH 4.5
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
700.0 μl of water (100 v/v)
F4
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 10,000 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 10,000 (50 w/v)
580.0 μl of water (100 v/v)
F5
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 10,000 20 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
F6
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 20,000 18 w/v -
pH 5
600.0 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
F7
  • Imidazole 0.1 M pH 7
  • Polyethylene glycol 20,000 12 w/v -
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
F8
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 20,000 8 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
633.3 μl of water (100 v/v)