Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG Rx 1
Barcodes
PEG Rx 1
Screen Structure
PEG Rx 1
Well Count | 48 |
---|---|
Number of distinct ingredients | 25 |
Internal diversity (score mean dist) | 0.948 |
Screen Composition
ingredient type | PEG Rx 1 |
---|---|
Buffer | 48 |
Organic (non-volatile) | 6 |
Polymer | 48 |
Salt | 20 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 340 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 560 μl of water (100 v/v) |
A2 |
| pH 5.5 | 380 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 520 μl of water (100 v/v) |
A3 |
| pH 7.5 | 420 μl of Polyethylene glycol 200 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 480 μl of water (100 v/v) |
A4 |
| pH 4.5 | 300 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium acetate pH 4.5 (1 M) 600 μl of water (100 v/v) |
A5 |
| pH 6.5 | 250 μl of Polyethylene glycol 300 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 650 μl of water (100 v/v) |
A6 |
| pH 8.5 | 200 μl of Polyethylene glycol 300 (100 v/v) 100 μl of BICINE pH 8.5 (1 M) 700 μl of water (100 v/v) |
A7 |
| pH 4 | 150 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Sodium acetate pH 4 (1 M) 750 μl of water (100 v/v) |
A8 |
| pH 6 | 100 μl of MES pH 6 (1 M) 220 μl of Polyethylene glycol 400 (100 v/v) 680 μl of water (100 v/v) |
B1 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 600 μl of water (100 v/v) |
B2 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 600 μl of water (100 v/v) |
B3 |
| pH 7 | 250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Imidazole pH 7 (1 M) 650 μl of water (100 v/v) |
B4 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 700 μl of water (100 v/v) |
B5 |
| pH 4 | 200 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of Sodium acetate pH 4 (1 M) 700 μl of water (100 v/v) |
B6 |
| pH 6 | 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of MES pH 6 (1 M) 500 μl of water (100 v/v) |
B7 |
| pH 8 | 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of water (100 v/v) |
B8 |
| pH 3.5 | 280 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 620 μl of water (100 v/v) |
C1 |
| pH 5.5 | 440 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 460 μl of water (100 v/v) |
C2 |
| pH 7.5 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |
C3 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 600 μl of Polyethylene glycol 1,500 (50 w/v) 300 μl of water (100 v/v) |
C4 |
| pH 6.5 | 400 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of water (100 v/v) |
C5 |
| pH 8.5 | 300 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of BICINE pH 8.5 (1 M) 600 μl of water (100 v/v) |
C6 |
| pH 4 | 200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Sodium acetate pH 4 (1 M) 700 μl of water (100 v/v) |
C7 |
| pH 6 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 500 μl of water (100 v/v) |
C8 |
| pH 8 | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of water (100 v/v) |
D1 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 600 μl of Jeffamine® ED-2001 (50 w/v) 300 μl of water (100 v/v) |
D2 |
| pH 7 | 400 μl of Jeffamine® ED-2001 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 500 μl of water (100 v/v) |
D3 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 200 μl of Jeffamine® ED-2001 (50 w/v) 700 μl of water (100 v/v) |
D4 |
| pH 3.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 400 μl of water (100 v/v) |
D5 |
| pH 5.5 | 360 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 540 μl of water (100 v/v) |
D6 |
| pH 7.5 | 240 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 660 μl of water (100 v/v) |
D7 |
| pH 4 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4 (1 M) 700 μl of water (100 v/v) |
D8 |
| pH 6 | 280 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 620 μl of water (100 v/v) |
E1 |
| pH 8 | 560 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 340 μl of water (100 v/v) |
E2 |
| pH 4.5 | 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of Sodium acetate pH 4.5 (1 M) 300 μl of water (100 v/v) |
E3 |
| pH 6.5 | 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of water (100 v/v) |
E4 |
| pH 8.5 | 160 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of BICINE pH 8.5 (1 M) 740 μl of water (100 v/v) |
E5 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
E6 |
| pH 7 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 500 μl of water (100 v/v) |
E7 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 600 μl of Polyethylene glycol 6,000 (50 w/v) 300 μl of water (100 v/v) |
E8 |
| pH 3.5 | 100 μl of tri-Sodium citrate pH 3.5 (1 M) 560 μl of Polyethylene glycol 8,000 (50 w/v) 340 μl of water (100 v/v) |
F1 |
| pH 5.5 | 320 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 580 μl of water (100 v/v) |
F2 |
| pH 7.5 | 80 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 820 μl of water (100 v/v) |
F3 |
| pH 4.5 | 200 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of Sodium acetate pH 4.5 (1 M) 700 μl of water (100 v/v) |
F4 |
| pH 6.5 | 320 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 580 μl of water (100 v/v) |
F5 |
| pH 8.5 | 400 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of BICINE pH 8.5 (1 M) 500 μl of water (100 v/v) |
F6 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 600 μl of Polyethylene glycol 20,000 (30 w/v) 300 μl of water (100 v/v) |
F7 |
| pH 7 | 400 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of Imidazole pH 7 (1 M) 500 μl of water (100 v/v) |
F8 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 266 μl of Polyethylene glycol 20,000 (30 w/v) 633 μl of water (100 v/v) |