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Supplemental Information

Screen Description

Hampton Research / PEG Rx 1
Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count 48
Number of distinct ingredients 25
Internal diversity (Score mean dist)0.95
Internal diversity (Ion mean dist)0.95
Internal diversity (PEG mean dist)0.94
Internal diversity (Known pH mean dist)0.79
Internal diversity (Penalty mean dist)0.92

Screen Composition

Chemical classes bar chart
ingredient type PEG Rx 1
Buffer 48
Organic (non-volatile) 6
Polymer 48
Salt 20

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG Rx 1 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Polyethylene glycol 200 34 v/v -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
340.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
560.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 200 38 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
380.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
520.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 200 42 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
420.0 μl of Polyethylene glycol 200 (100 v/v)
480.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 300 30 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
600.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 300 25 v/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
250.0 μl of Polyethylene glycol 300 (100 v/v)
650.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 300 20 v/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
200.0 μl of Polyethylene glycol 300 (100 v/v)
700.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 400 15 v/v -
  • Sodium acetate 0.1 M pH 4
pH 4
150.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
750.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 400 22 v/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of MES pH 6 (1 M)
220.0 μl of Polyethylene glycol 400 (100 v/v)
680.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
600.0 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
600.0 μl of water (100 v/v)
B3
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Imidazole pH 7 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
650.0 μl of water (100 v/v)
B4
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
700.0 μl of water (100 v/v)
B5
  • Jeffamine® M-600 10 v/v -
  • Sodium acetate 0.1 M pH 4
pH 4
200.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
B6
  • Jeffamine® M-600 20 v/v -
  • MES 0.1 M pH 6
pH 6
400.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of MES pH 6 (1 M)
500.0 μl of water (100 v/v)
B7
  • Jeffamine® M-600 30 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 1,000 14 w/v -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
280.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
620.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 1,000 22 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
440.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
460.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 1,000 30 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
300.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 1,500 30 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 1,500 20 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 1,500 15 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
600.0 μl of water (100 v/v)
C6
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
  • Sodium acetate 0.1 M pH 4
pH 4
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
C7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
C8
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D1
  • Jeffamine® ED-2001 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
600.0 μl of Jeffamine® ED-2001 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
D2
  • Jeffamine® ED-2001 20 w/v -
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Jeffamine® ED-2001 (50 w/v)
500.0 μl of water (100 v/v)
D3
  • Jeffamine® ED-2001 10 w/v -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
200.0 μl of Jeffamine® ED-2001 (50 w/v)
700.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 25 w/v -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,350 18 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
360.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
540.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 12 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
660.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 4,000 10 w/v -
  • Sodium acetate 0.1 M pH 4
pH 4
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
700.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 4,000 14 w/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of MES pH 6 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
620.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 4,000 28 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
E2
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
E3
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
E4
  • Polyethylene glycol monomethyl ether 5,000 8 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
740.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 6,000 10 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
700.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 6,000 20 w/v -
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 6,000 30 w/v -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 8,000 28 w/v -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
560.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
340.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 8,000 16 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 8,000 4 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol 8,000 (50 w/v)
820.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 10,000 10 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
700.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 10,000 16 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 10,000 (50 w/v)
580.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 10,000 20 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 20,000 18 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
600.0 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 20,000 12 w/v -
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Imidazole pH 7 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 20,000 8 w/v -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
266.67 μl of Polyethylene glycol 20,000 (30 w/v)
633.33 μl of water (100 v/v)