Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG Rx 1
Barcodes
PEG Rx 1
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Screen Structure
PEG Rx 1
Well Count | 48 |
---|---|
Number of distinct ingredients | 25 |
Internal diversity (score mean dist) | 0.948 |
Screen Composition
ingredient type | PEG Rx 1 |
---|---|
Buffer | 48 |
Organic (non-volatile) | 6 |
Polymer | 48 |
Salt | 20 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 340.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 560.0 μl of water (100 v/v) |
A2 |
| pH 5.5 | 380.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 520.0 μl of water (100 v/v) |
A3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 420.0 μl of Polyethylene glycol 200 (100 v/v) 480.0 μl of water (100 v/v) |
A4 |
| pH 4.5 | 300.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 600.0 μl of water (100 v/v) |
A5 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 250.0 μl of Polyethylene glycol 300 (100 v/v) 650.0 μl of water (100 v/v) |
A6 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 200.0 μl of Polyethylene glycol 300 (100 v/v) 700.0 μl of water (100 v/v) |
A7 |
| pH 4 | 150.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4 (1 M) 750.0 μl of water (100 v/v) |
A8 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 220.0 μl of Polyethylene glycol 400 (100 v/v) 680.0 μl of water (100 v/v) |
B1 |
| pH 8 | 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 600.0 μl of water (100 v/v) |
B2 |
| pH 5 | 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 600.0 μl of water (100 v/v) |
B3 |
| pH 7 | 100.0 μl of Imidazole pH 7 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 650.0 μl of water (100 v/v) |
B4 |
| pH 9 | 100.0 μl of BIS-TRIS propane pH 9 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 700.0 μl of water (100 v/v) |
B5 |
| pH 4 | 200.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of Sodium acetate pH 4 (1 M) 700.0 μl of water (100 v/v) |
B6 |
| pH 6 | 400.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of MES pH 6 (1 M) 500.0 μl of water (100 v/v) |
B7 |
| pH 8 | 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
B8 |
| pH 3.5 | 280.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 620.0 μl of water (100 v/v) |
C1 |
| pH 5.5 | 440.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 460.0 μl of water (100 v/v) |
C2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 1,000 (50 w/v) 300.0 μl of water (100 v/v) |
C3 |
| pH 4.5 | 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 300.0 μl of water (100 v/v) |
C4 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
C5 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 300.0 μl of Polyethylene glycol 1,500 (50 w/v) 600.0 μl of water (100 v/v) |
C6 |
| pH 4 | 200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4 (1 M) 700.0 μl of water (100 v/v) |
C7 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of water (100 v/v) |
C8 |
| pH 8 | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
D1 |
| pH 5 | 600.0 μl of Jeffamine® ED-2001 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
D2 |
| pH 7 | 100.0 μl of Imidazole pH 7 (1 M) 400.0 μl of Jeffamine® ED-2001 (50 w/v) 500.0 μl of water (100 v/v) |
D3 |
| pH 9 | 100.0 μl of BIS-TRIS propane pH 9 (1 M) 200.0 μl of Jeffamine® ED-2001 (50 w/v) 700.0 μl of water (100 v/v) |
D4 |
| pH 3.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 400.0 μl of water (100 v/v) |
D5 |
| pH 5.5 | 360.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 540.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 660.0 μl of water (100 v/v) |
D7 |
| pH 4 | 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4 (1 M) 700.0 μl of water (100 v/v) |
D8 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 280.0 μl of Polyethylene glycol 4,000 (50 w/v) 620.0 μl of water (100 v/v) |
E1 |
| pH 8 | 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 340.0 μl of water (100 v/v) |
E2 |
| pH 4.5 | 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 300.0 μl of water (100 v/v) |
E3 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 740.0 μl of water (100 v/v) |
E5 |
| pH 5 | 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 700.0 μl of water (100 v/v) |
E6 |
| pH 7 | 100.0 μl of Imidazole pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
E7 |
| pH 9 | 100.0 μl of BIS-TRIS propane pH 9 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
E8 |
| pH 3.5 | 560.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 340.0 μl of water (100 v/v) |
F1 |
| pH 5.5 | 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 580.0 μl of water (100 v/v) |
F2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Polyethylene glycol 8,000 (50 w/v) 820.0 μl of water (100 v/v) |
F3 |
| pH 4.5 | 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 700.0 μl of water (100 v/v) |
F4 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 320.0 μl of Polyethylene glycol 10,000 (50 w/v) 580.0 μl of water (100 v/v) |
F5 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v) |
F6 |
| pH 5 | 600.0 μl of Polyethylene glycol 20,000 (30 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
F7 |
| pH 7 | 100.0 μl of Imidazole pH 7 (1 M) 400.0 μl of Polyethylene glycol 20,000 (30 w/v) 500.0 μl of water (100 v/v) |
F8 |
| pH 9 | 100.0 μl of BIS-TRIS propane pH 9 (1 M) 266.7 μl of Polyethylene glycol 20,000 (30 w/v) 633.3 μl of water (100 v/v) |