Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

PEG Rx 1

Hampton Research / PEG Rx 1
Created 2009-11-16.

Export

Barcodes

PEG Rx 1

    Screen Structure

    PEG Rx 1

    Well Count 48
    Number of distinct ingredients 25
    Internal diversity (score mean dist) 0.948

    Screen Composition

    2025-06-13T21:33:17.397465 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Rx 1
    Buffer 48
    Organic (non-volatile) 6
    Polymer 48
    Salt 20

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Rx 1 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 200 34 v/v -
    pH 3.5
    340 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    560 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 200 38 v/v -
    pH 5.5
    380 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    520 μl of water (100 v/v)
    A3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 42 v/v -
    pH 7.5
    420 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 30 v/v -
    pH 4.5
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    600 μl of water (100 v/v)
    A5
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 300 25 v/v -
    pH 6.5
    250 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    650 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 300 20 v/v -
    pH 8.5
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BICINE pH 8.5 (1 M)
    700 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 400 15 v/v -
    pH 4
    150 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4 (1 M)
    750 μl of water (100 v/v)
    A8
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 22 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    680 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    600 μl of water (100 v/v)
    B3
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    pH 7
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Imidazole pH 7 (1 M)
    650 μl of water (100 v/v)
    B4
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    700 μl of water (100 v/v)
    B5
    • Sodium acetate 0.1 M pH 4
    • Jeffamine® M-600 10 v/v -
    pH 4
    200 μl of Jeffamine® M-600 pH 7 (50 v/v)
    100 μl of Sodium acetate pH 4 (1 M)
    700 μl of water (100 v/v)
    B6
    • MES 0.1 M pH 6
    • Jeffamine® M-600 20 v/v -
    pH 6
    400 μl of Jeffamine® M-600 pH 7 (50 v/v)
    100 μl of MES pH 6 (1 M)
    500 μl of water (100 v/v)
    B7
    • tris chloride BCC 0.1 M pH 8
    • Jeffamine® M-600 30 v/v -
    pH 8
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 1,000 14 w/v -
    pH 3.5
    280 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    620 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 22 w/v -
    pH 5.5
    440 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    460 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 30 w/v -
    pH 7.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,500 30 w/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of water (100 v/v)
    C4
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,500 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of water (100 v/v)
    C5
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 1,500 15 w/v -
    pH 8.5
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    600 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 4
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    700 μl of water (100 v/v)
    C7
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    500 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 8
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 5
    • Jeffamine® ED-2001 30 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Jeffamine® ED-2001 (50 w/v)
    300 μl of water (100 v/v)
    D2
    • Imidazole 0.1 M pH 7
    • Jeffamine® ED-2001 20 w/v -
    pH 7
    400 μl of Jeffamine® ED-2001 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    500 μl of water (100 v/v)
    D3
    • BIS-TRIS propane 0.1 M pH 9
    • Jeffamine® ED-2001 10 w/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    200 μl of Jeffamine® ED-2001 (50 w/v)
    700 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 3.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    400 μl of water (100 v/v)
    D5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 18 w/v -
    pH 5.5
    360 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    540 μl of water (100 v/v)
    D6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 12 w/v -
    pH 7.5
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    660 μl of water (100 v/v)
    D7
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 4,000 10 w/v -
    pH 4
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    700 μl of water (100 v/v)
    D8
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 14 w/v -
    pH 6
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    620 μl of water (100 v/v)
    E1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 28 w/v -
    pH 8
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    340 μl of water (100 v/v)
    E2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 4.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of water (100 v/v)
    E3
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of water (100 v/v)
    E4
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 8 w/v -
    pH 8.5
    160 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    740 μl of water (100 v/v)
    E5
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    E6
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    500 μl of water (100 v/v)
    E7
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 6,000 30 w/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    E8
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 8,000 28 w/v -
    pH 3.5
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    560 μl of Polyethylene glycol 8,000 (50 w/v)
    340 μl of water (100 v/v)
    F1
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 16 w/v -
    pH 5.5
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    580 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 4 w/v -
    pH 7.5
    80 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    F3
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 4.5
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    700 μl of water (100 v/v)
    F4
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 10,000 16 w/v -
    pH 6.5
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    580 μl of water (100 v/v)
    F5
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 10,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    500 μl of water (100 v/v)
    F6
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 20,000 18 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    600 μl of Polyethylene glycol 20,000 (30 w/v)
    300 μl of water (100 v/v)
    F7
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 20,000 12 w/v -
    pH 7
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Imidazole pH 7 (1 M)
    500 μl of water (100 v/v)
    F8
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 20,000 8 w/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    633 μl of water (100 v/v)