C6 - Screen Contents

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Supplemental Information

Screen Description

PEG Rx 2

Hampton Research / PEG Rx 2

Created 2009-11-16.

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PEG Rx 2

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PEG Rx 2

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Screen Structure

PEG Rx 2

Well Count	48
Number of distinct ingredients	46
Internal diversity (score mean dist)	0.952

Screen Composition

More information about screen composition chart.

ingredient type	PEG Rx 2
Buffer	63
Complex ingredient	4
Detergent	1
Organic (non-volatile)	8
Organic (volatile)	13
Polymer	50
Salt	49

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

PEG Rx 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Lithium sulfate 0.8 M - Sodium acetate 0.1 M pH 4 Polyethylene glycol 200 4 v/v - 🔍	pH 4	400.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Sodium acetate pH 4 (1 M) 460.0 μl of water (100 v/v)
A2	Lithium sulfate 0.2 M - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 200 26 v/v - 🔍	pH 5	100.0 μl of Lithium sulfate (2 M) 260.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 540.0 μl of water (100 v/v)
A3	Calcium chloride 0.05 M - MES 0.1 M pH 6 Polyethylene glycol 200 45 v/v - 🔍	pH 6	25.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 450.0 μl of Polyethylene glycol 200 (100 v/v) 425.0 μl of water (100 v/v)
A4	2-Propanol 28 v/v - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 200 3 v/v - 🔍	pH 6.5	280.0 μl of 2-Propanol (100 v/v) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 30.0 μl of Polyethylene glycol 200 (100 v/v) 590.0 μl of water (100 v/v)
A5	Tacsimate 20 v/v pH 7 HEPES 0.1 M pH 7.5 Polyethylene glycol 200 2 v/v - 🔍	NA	100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polyethylene glycol 200 (100 v/v) 200.0 μl of Tacsimate pH 7 (100 v/v) 680.0 μl of water (100 v/v)
A6	2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 400 26 v/v - 🔍	pH 5	100.0 μl of 2-Propanol (100 v/v) 260.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 540.0 μl of water (100 v/v)
A7	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.5 Polyethylene glycol 400 24 v/v - 🔍	pH 5.5	200.0 μl of Ammonium acetate (1 M) 240.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 460.0 μl of water (100 v/v)
A8	Ammonium sulfate 0.2 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 400 18 v/v - 🔍	pH 6.5	57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 180.0 μl of Polyethylene glycol 400 (100 v/v) 662.9 μl of water (100 v/v)
B1	HEPES 0.1 M pH 7.5 Polyethylene glycol 400 40 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 500.0 μl of water (100 v/v)
B2	2-Propanol 6 v/v - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol monomethyl ether 550 26 v/v - 🔍	pH 4.5	60.0 μl of 2-Propanol (100 v/v) 260.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 580.0 μl of water (100 v/v)
B3	Ammonium sulfate 1.8 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 2 v/v - 🔍	pH 6.5	514.3 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 365.7 μl of water (100 v/v)
B4	di-Sodium DL-malate 0.15 M - Imidazole 0.1 M pH 7 Polyethylene glycol monomethyl ether 550 22 v/v - 🔍	pH 7	100.0 μl of Imidazole pH 7 (1 M) 220.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50.0 μl of di-Sodium DL-malate (3 M) 630.0 μl of water (100 v/v)
B5	di-Sodium succinate 0.1 M - BICINE 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 550 30 v/v - 🔍	pH 8.5	100.0 μl of BICINE pH 8.5 (1 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 83.3 μl of di-Sodium succinate (1.2 M) 516.7 μl of water (100 v/v)
B6	Lithium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.5 Polyethylene glycol 1,000 20 w/v - 🔍	pH 5.5	50.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 450.0 μl of water (100 v/v)
B7	di-Sodium malonate 0.1 M - tris chloride BCC 0.1 M pH 8 Polyethylene glycol 1,000 30 w/v - 🔍	pH 8	600.0 μl of Polyethylene glycol 1,000 (50 w/v) 29.4 μl of di-Sodium malonate pH 8 (3.4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 270.6 μl of water (100 v/v)
B8	2-methyl-2,4-pentanediol BCC 4 v/v - tri-Sodium citrate 0.1 M pH 3.5 Polyethylene glycol 1,500 20 w/v - 🔍	pH 3.5	40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 460.0 μl of water (100 v/v)
C1	L-Proline 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 1,500 24 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of L-Proline (1 M) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 220.0 μl of water (100 v/v)
C2	2-Propanol 10 v/v - BICINE 0.1 M pH 8.5 Polyethylene glycol 1,500 30 w/v - 🔍	pH 8.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of BICINE pH 8.5 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 200.0 μl of water (100 v/v)
C3	Sodium chloride 0.1 M - BIS-TRIS propane 0.1 M pH 9 Polyethylene glycol 1,500 25 w/v - 🔍	pH 9	100.0 μl of BIS-TRIS propane pH 9 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 20.0 μl of Sodium chloride (5 M) 380.0 μl of water (100 v/v)
C4	Nickel (II) chloride 0.02 M - Magnesium chloride 0.02 M - Cadmium chloride 0.02 M - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol monomethyl ether 2,000 24 w/v - 🔍	pH 4.5	20.0 μl of Cadmium chloride (1 M) 10.0 μl of Magnesium chloride (2 M) 5.0 μl of Nickel (II) chloride (4 M) 480.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 385.0 μl of water (100 v/v)
C5	2-Propanol 20 v/v - MES 0.1 M pH 6 Polyethylene glycol monomethyl ether 2,000 20 w/v - 🔍	pH 6	200.0 μl of 2-Propanol (100 v/v) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 300.0 μl of water (100 v/v)
C6	tri-Ammonium citrate 0.2 M - Imidazole 0.1 M pH 7 Polyethylene glycol monomethyl ether 2,000 20 w/v - 🔍	pH 7	100.0 μl of Imidazole pH 7 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 80.0 μl of tri-Ammonium citrate (2.5 M) 420.0 μl of water (100 v/v)
C7	Potassium formate 4 M - BIS-TRIS propane 0.1 M pH 9 Polyethylene glycol monomethyl ether 2,000 2 w/v - 🔍	pH 9	100.0 μl of BIS-TRIS propane pH 9 (1 M) 40.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 285.7 μl of Potassium formate (14 M) 574.3 μl of water (100 v/v)
C8	Tacsimate 50 v/v pH 4 Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 3,350 1 w/v - 🔍	NA	20.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of Tacsimate pH 4 (100 v/v) 380.0 μl of water (100 v/v)
D1	n-Octyl-ß-D-glucoside 0.1 w/v - tri-Sodium citrate 0.1 M pH 5.5 Polyethylene glycol 3,350 22 w/v - 🔍	pH 5.5	440.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of n-Octyl-ß-D-glucoside (5 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 440.0 μl of water (100 v/v)
D2	Tacsimate 2 v/v pH 7 2-Propanol 5 v/v - Imidazole 0.1 M pH 7 Polyethylene glycol 3,350 8 w/v - 🔍	NA	50.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 7 (1 M) 160.0 μl of Polyethylene glycol 3,350 (50 w/v) 20.0 μl of Tacsimate pH 7 (100 v/v) 670.0 μl of water (100 v/v)
D3	1,4-Dioxane 2 v/v - tris chloride BCC 0.1 M pH 8 Polyethylene glycol 3,350 15 w/v - 🔍	pH 8	20.0 μl of 1,4-Dioxane (100 v/v) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 580.0 μl of water (100 v/v)
D4	2-Propanol 18 v/v - tri-Sodium citrate 0.1 M pH 5.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 5.5	180.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 320.0 μl of water (100 v/v)
D5	Tacsimate 6 v/v pH 6 MES 0.1 M pH 6 Polyethylene glycol 4,000 25 w/v - 🔍	NA	100.0 μl of MES pH 6 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 60.0 μl of Tacsimate pH 6 (100 v/v) 340.0 μl of water (100 v/v)
D6	Magnesium formate 0.2 M - Sodium acetate 0.1 M pH 4 Polyethylene glycol monomethyl ether 5,000 18 w/v - 🔍	pH 4	200.0 μl of Magnesium formate (1 M) 360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of Sodium acetate pH 4 (1 M) 340.0 μl of water (100 v/v)
D7	Polyethylene glycol 400 2 v/v - Imidazole 0.1 M pH 7 Polyethylene glycol monomethyl ether 5,000 24 w/v - 🔍	pH 7	100.0 μl of Imidazole pH 7 (1 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 480.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 400.0 μl of water (100 v/v)
D8	Sodium formate 0.2 M - BICINE 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 5,000 20 w/v - 🔍	pH 8.5	100.0 μl of BICINE pH 8.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 28.6 μl of Sodium formate (7 M) 471.4 μl of water (100 v/v)
E1	2-Propanol 4 v/v - BIS-TRIS propane 0.1 M pH 9 Polyethylene glycol monomethyl ether 5,000 20 w/v - 🔍	pH 9	40.0 μl of 2-Propanol (100 v/v) 100.0 μl of BIS-TRIS propane pH 9 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 460.0 μl of water (100 v/v)
E2	Ethylene glycol 6 v/v - tri-Sodium citrate 0.1 M pH 3.5 Polyethylene glycol 6,000 10 w/v - 🔍	pH 3.5	60.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 640.0 μl of water (100 v/v)
E3	Lithium sulfate 0.15 M - tri-Sodium citrate 0.1 M pH 3.5 Polyethylene glycol 6,000 18 w/v - 🔍	pH 3.5	75.0 μl of Lithium sulfate (2 M) 360.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 465.0 μl of water (100 v/v)
E4	2-Propanol 10 v/v - Sodium acetate 0.1 M pH 4 Polyethylene glycol 6,000 22 w/v - 🔍	pH 4	100.0 μl of 2-Propanol (100 v/v) 440.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium acetate pH 4 (1 M) 360.0 μl of water (100 v/v)
E5	Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4 Polyethylene glycol 8,000 22 w/v - 🔍	pH 4	440.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4 (1 M) 40.0 μl of Sodium chloride (5 M) 420.0 μl of water (100 v/v)
E6	2-Propanol 20 v/v - tris chloride BCC 0.1 M pH 8 Polyethylene glycol 8,000 5 w/v - 🔍	pH 8	200.0 μl of 2-Propanol (100 v/v) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 600.0 μl of water (100 v/v)
E7	Polyethylene glycol 200 10 v/v - BIS-TRIS propane 0.1 M pH 9 Polyethylene glycol 8,000 18 w/v - 🔍	pH 9	100.0 μl of BIS-TRIS propane pH 9 (1 M) 100.0 μl of Polyethylene glycol 200 (100 v/v) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 440.0 μl of water (100 v/v)
E8	2-Propanol 15 v/v - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 10,000 10 w/v - 🔍	pH 5	150.0 μl of 2-Propanol (100 v/v) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 550.0 μl of water (100 v/v)
F1	di-Sodium malonate 0.4 M - MES 0.1 M pH 6 Polyethylene glycol 10,000 0.5 w/v - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 10.0 μl of Polyethylene glycol 10,000 (50 w/v) 117.6 μl of di-Sodium malonate pH 6 (3.4 M) 772.4 μl of water (100 v/v)
F2	Potassium Sodium tartrate 0.2 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 10,000 10 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 133.3 μl of Potassium Sodium tartrate (1.5 M) 566.7 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 5 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 10 w/v - 🔍	pH 7.5	50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 650.0 μl of water (100 v/v)
F4	Ammonium acetate 0.2 M - tris chloride BCC 0.1 M pH 8 Polyethylene glycol 10,000 16 w/v - 🔍	pH 8	200.0 μl of Ammonium acetate (1 M) 320.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 380.0 μl of water (100 v/v)
F5	2-Propanol 5 v/v - tri-Sodium citrate 0.1 M pH 3.5 Polyethylene glycol 20,000 6 w/v - 🔍	pH 3.5	50.0 μl of 2-Propanol (100 v/v) 200.0 μl of Polyethylene glycol 20,000 (30 w/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 650.0 μl of water (100 v/v)
F6	di-Sodium malonate 1 M - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 20,000 2 w/v - 🔍	pH 4.5	66.7 μl of Polyethylene glycol 20,000 (30 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 294.1 μl of di-Sodium malonate pH 5 (3.4 M) 539.2 μl of water (100 v/v)
F7	Magnesium chloride 0.2 M - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 20,000 10 w/v - 🔍	pH 5	100.0 μl of Magnesium chloride (2 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 466.7 μl of water (100 v/v)
F8	Dextran sulfate sodium salt 5,000 3 w/v - BICINE 0.1 M pH 8.5 Polyethylene glycol 20,000 15 w/v - 🔍	pH 8.5	100.0 μl of BICINE pH 8.5 (1 M) 100.0 μl of Dextran sulfate sodium salt 5,000 (30 w/v) 500.0 μl of Polyethylene glycol 20,000 (30 w/v) 300.0 μl of water (100 v/v)