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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG Rx 2
Barcodes
PEG Rx 2
Screen Structure
PEG Rx 2
Well Count | 48 |
---|---|
Number of distinct ingredients | 46 |
Internal diversity (score mean dist) | 0.952 |
Screen Composition
ingredient type | PEG Rx 2 |
---|---|
Buffer | 63 |
Complex ingredient | 4 |
Detergent | 1 |
Organic (non-volatile) | 8 |
Organic (volatile) | 13 |
Polymer | 50 |
Salt | 49 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4 | 100 μl of Sodium acetate pH 4 (1 M) 40 μl of Polyethylene glycol 200 (100 v/v) 400 μl of Lithium sulfate (2 M) 460 μl of water (100 v/v) |
A2 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 260 μl of Polyethylene glycol 200 (100 v/v) 100 μl of Lithium sulfate (2 M) 540 μl of water (100 v/v) |
A3 |
| pH 6 | 450 μl of Polyethylene glycol 200 (100 v/v) 25 μl of Calcium chloride (2 M) 100 μl of MES pH 6 (1 M) 425 μl of water (100 v/v) |
A4 |
| pH 6.5 | 30 μl of Polyethylene glycol 200 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 280 μl of 2-Propanol (100 v/v) 590 μl of water (100 v/v) |
A5 |
| NA | 200 μl of Tacsimate pH 7 (100 v/v) 20 μl of Polyethylene glycol 200 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 680 μl of water (100 v/v) |
A6 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 100 μl of 2-Propanol (100 v/v) 260 μl of Polyethylene glycol 400 (100 v/v) 540 μl of water (100 v/v) |
A7 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 240 μl of Polyethylene glycol 400 (100 v/v) 460 μl of water (100 v/v) |
A8 |
| pH 6.5 | 100 μl of BIS-TRIS pH 6.5 (1 M) 57 μl of Ammonium sulfate (3.5 M) 180 μl of Polyethylene glycol 400 (100 v/v) 662 μl of water (100 v/v) |
B1 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 500 μl of water (100 v/v) |
B2 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 60 μl of 2-Propanol (100 v/v) 580 μl of water (100 v/v) |
B3 |
| pH 6.5 | 20 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 514 μl of Ammonium sulfate (3.5 M) 365 μl of water (100 v/v) |
B4 |
| pH 7 | 220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Imidazole pH 7 (1 M) 50 μl of di-Sodium DL-malate (3 M) 630 μl of water (100 v/v) |
B5 |
| pH 8.5 | 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 83 μl of di-Sodium succinate (1.2 M) 100 μl of BICINE pH 8.5 (1 M) 516 μl of water (100 v/v) |
B6 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 50 μl of Lithium sulfate (2 M) 450 μl of water (100 v/v) |
B7 |
| pH 8 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 29 μl of di-Sodium malonate pH 8 (3.4 M) 100 μl of tris chloride BCC pH 8 (1 M) 270 μl of water (100 v/v) |
B8 |
| pH 3.5 | 400 μl of Polyethylene glycol 1,500 (50 w/v) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 460 μl of water (100 v/v) |
C1 |
| pH 7.5 | 200 μl of L-Proline (1 M) 480 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 220 μl of water (100 v/v) |
C2 |
| pH 8.5 | 600 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of 2-Propanol (100 v/v) 100 μl of BICINE pH 8.5 (1 M) 200 μl of water (100 v/v) |
C3 |
| pH 9 | 20 μl of Sodium chloride (5 M) 100 μl of BIS-TRIS propane pH 9 (1 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 380 μl of water (100 v/v) |
C4 |
| pH 4.5 | 100 μl of Sodium acetate pH 4.5 (1 M) 10 μl of Magnesium chloride (2 M) 480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 20 μl of Cadmium chloride (1 M) 5 μl of Nickel (II) chloride (4 M) 385 μl of water (100 v/v) |
C5 |
| pH 6 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 100 μl of MES pH 6 (1 M) 300 μl of water (100 v/v) |
C6 |
| pH 7 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 80 μl of tri-Ammonium citrate (2.5 M) 420 μl of water (100 v/v) |
C7 |
| pH 9 | 40 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 285 μl of Potassium formate (14 M) 574 μl of water (100 v/v) |
C8 |
| NA | 100 μl of Sodium acetate pH 4.5 (1 M) 20 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of Tacsimate pH 4 (100 v/v) 380 μl of water (100 v/v) |
D1 |
| pH 5.5 | 20 μl of n-Octyl-ß-D-glucoside (5 w/v) 440 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 440 μl of water (100 v/v) |
D2 |
| NA | 20 μl of Tacsimate pH 7 (100 v/v) 160 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 50 μl of 2-Propanol (100 v/v) 670 μl of water (100 v/v) |
D3 |
| pH 8 | 300 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of 1,4-Dioxane (100 v/v) 100 μl of tris chloride BCC pH 8 (1 M) 580 μl of water (100 v/v) |
D4 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 180 μl of 2-Propanol (100 v/v) 400 μl of Polyethylene glycol 4,000 (50 w/v) 320 μl of water (100 v/v) |
D5 |
| NA | 60 μl of Tacsimate pH 6 (100 v/v) 100 μl of MES pH 6 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 340 μl of water (100 v/v) |
D6 |
| pH 4 | 200 μl of Magnesium formate (1 M) 100 μl of Sodium acetate pH 4 (1 M) 360 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 340 μl of water (100 v/v) |
D7 |
| pH 7 | 480 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of Imidazole pH 7 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 400 μl of water (100 v/v) |
D8 |
| pH 8.5 | 28 μl of Sodium formate (7 M) 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of BICINE pH 8.5 (1 M) 471 μl of water (100 v/v) |
E1 |
| pH 9 | 100 μl of BIS-TRIS propane pH 9 (1 M) 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 40 μl of 2-Propanol (100 v/v) 460 μl of water (100 v/v) |
E2 |
| pH 3.5 | 60 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 640 μl of water (100 v/v) |
E3 |
| pH 3.5 | 360 μl of Polyethylene glycol 6,000 (50 w/v) 75 μl of Lithium sulfate (2 M) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 465 μl of water (100 v/v) |
E4 |
| pH 4 | 100 μl of Sodium acetate pH 4 (1 M) 440 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of 2-Propanol (100 v/v) 360 μl of water (100 v/v) |
E5 |
| pH 4 | 100 μl of Sodium acetate pH 4 (1 M) 40 μl of Sodium chloride (5 M) 440 μl of Polyethylene glycol 8,000 (50 w/v) 420 μl of water (100 v/v) |
E6 |
| pH 8 | 100 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 200 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v) |
E7 |
| pH 9 | 100 μl of Polyethylene glycol 200 (100 v/v) 100 μl of BIS-TRIS propane pH 9 (1 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 440 μl of water (100 v/v) |
E8 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 200 μl of Polyethylene glycol 10,000 (50 w/v) 150 μl of 2-Propanol (100 v/v) 550 μl of water (100 v/v) |
F1 |
| pH 6 | 10 μl of Polyethylene glycol 10,000 (50 w/v) 117 μl of di-Sodium malonate pH 6 (3.4 M) 100 μl of MES pH 6 (1 M) 772 μl of water (100 v/v) |
F2 |
| pH 6.5 | 133 μl of Potassium Sodium tartrate (1.5 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 200 μl of Polyethylene glycol 10,000 (50 w/v) 566 μl of water (100 v/v) |
F3 |
| pH 7.5 | 200 μl of Polyethylene glycol 10,000 (50 w/v) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 650 μl of water (100 v/v) |
F4 |
| pH 8 | 200 μl of Ammonium acetate (1 M) 320 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 380 μl of water (100 v/v) |
F5 |
| pH 3.5 | 200 μl of Polyethylene glycol 20,000 (30 w/v) 50 μl of 2-Propanol (100 v/v) 100 μl of tri-Sodium citrate pH 3.5 (1 M) 650 μl of water (100 v/v) |
F6 |
| pH 4.5 | 294 μl of di-Sodium malonate pH 5 (3.4 M) 100 μl of Sodium acetate pH 4.5 (1 M) 66 μl of Polyethylene glycol 20,000 (30 w/v) 539 μl of water (100 v/v) |
F7 |
| pH 5 | 100 μl of Magnesium chloride (2 M) 100 μl of tri-Sodium citrate pH 5 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 466 μl of water (100 v/v) |
F8 |
| pH 8.5 | 500 μl of Polyethylene glycol 20,000 (30 w/v) 100 μl of Dextran sulfate sodium salt 5,000 (30 w/v) 100 μl of BICINE pH 8.5 (1 M) 300 μl of water (100 v/v) |