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Supplemental Information

Screen Description

PEG Rx 2

Hampton Research / PEG Rx 2
Created 2009-11-16.

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PEG Rx 2

    Screen Structure

    PEG Rx 2

    Well Count 48
    Number of distinct ingredients 46
    Internal diversity (score mean dist) 0.952

    Screen Composition

    2025-06-13T21:33:17.064725 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Rx 2
    Buffer 63
    Complex ingredient 4
    Detergent 1
    Organic (non-volatile) 8
    Organic (volatile) 13
    Polymer 50
    Salt 49

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Rx 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium sulfate 0.8 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 200 4 v/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    40 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of Lithium sulfate (2 M)
    460 μl of water (100 v/v)
    A2
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 26 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    260 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    540 μl of water (100 v/v)
    A3
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 45 v/v -
    pH 6
    450 μl of Polyethylene glycol 200 (100 v/v)
    25 μl of Calcium chloride (2 M)
    100 μl of MES pH 6 (1 M)
    425 μl of water (100 v/v)
    A4
    • 2-Propanol 28 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 200 3 v/v -
    pH 6.5
    30 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    280 μl of 2-Propanol (100 v/v)
    590 μl of water (100 v/v)
    A5
    • Tacsimate 20 v/v pH 7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 2 v/v -
    NA
    200 μl of Tacsimate pH 7 (100 v/v)
    20 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    680 μl of water (100 v/v)
    A6
    • 2-Propanol 10 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 26 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    260 μl of Polyethylene glycol 400 (100 v/v)
    540 μl of water (100 v/v)
    A7
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 24 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    460 μl of water (100 v/v)
    A8
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 18 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    180 μl of Polyethylene glycol 400 (100 v/v)
    662 μl of water (100 v/v)
    B1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 40 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    B2
    • 2-Propanol 6 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    60 μl of 2-Propanol (100 v/v)
    580 μl of water (100 v/v)
    B3
    • Ammonium sulfate 1.8 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 2 v/v -
    pH 6.5
    20 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    365 μl of water (100 v/v)
    B4
    • di-Sodium DL-malate 0.15 M -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 7
    220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Imidazole pH 7 (1 M)
    50 μl of di-Sodium DL-malate (3 M)
    630 μl of water (100 v/v)
    B5
    • di-Sodium succinate 0.1 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    83 μl of di-Sodium succinate (1.2 M)
    100 μl of BICINE pH 8.5 (1 M)
    516 μl of water (100 v/v)
    B6
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 20 w/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of Lithium sulfate (2 M)
    450 μl of water (100 v/v)
    B7
    • di-Sodium malonate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 1,000 30 w/v -
    pH 8
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    29 μl of di-Sodium malonate pH 8 (3.4 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    270 μl of water (100 v/v)
    B8
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 1,500 20 w/v -
    pH 3.5
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    460 μl of water (100 v/v)
    C1
    • L-Proline 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,500 24 w/v -
    pH 7.5
    200 μl of L-Proline (1 M)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    220 μl of water (100 v/v)
    C2
    • 2-Propanol 10 v/v -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 1,500 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    100 μl of BICINE pH 8.5 (1 M)
    200 μl of water (100 v/v)
    C3
    • Sodium chloride 0.1 M -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 1,500 25 w/v -
    pH 9
    20 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    380 μl of water (100 v/v)
    C4
    • Nickel (II) chloride 0.02 M -
    • Magnesium chloride 0.02 M -
    • Cadmium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 2,000 24 w/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    10 μl of Magnesium chloride (2 M)
    480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    20 μl of Cadmium chloride (1 M)
    5 μl of Nickel (II) chloride (4 M)
    385 μl of water (100 v/v)
    C5
    • 2-Propanol 20 v/v -
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    100 μl of MES pH 6 (1 M)
    300 μl of water (100 v/v)
    C6
    • tri-Ammonium citrate 0.2 M -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    80 μl of tri-Ammonium citrate (2.5 M)
    420 μl of water (100 v/v)
    C7
    • Potassium formate 4 M -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 2,000 2 w/v -
    pH 9
    40 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    285 μl of Potassium formate (14 M)
    574 μl of water (100 v/v)
    C8
    • Tacsimate 50 v/v pH 4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 1 w/v -
    NA
    100 μl of Sodium acetate pH 4.5 (1 M)
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of Tacsimate pH 4 (100 v/v)
    380 μl of water (100 v/v)
    D1
    • n-Octyl-ß-D-glucoside 0.1 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 22 w/v -
    pH 5.5
    20 μl of n-Octyl-ß-D-glucoside (5 w/v)
    440 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    440 μl of water (100 v/v)
    D2
    • Tacsimate 2 v/v pH 7
    • 2-Propanol 5 v/v -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol 3,350 8 w/v -
    NA
    20 μl of Tacsimate pH 7 (100 v/v)
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    50 μl of 2-Propanol (100 v/v)
    670 μl of water (100 v/v)
    D3
    • 1,4-Dioxane 2 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 3,350 15 w/v -
    pH 8
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of 1,4-Dioxane (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    580 μl of water (100 v/v)
    D4
    • 2-Propanol 18 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    180 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    320 μl of water (100 v/v)
    D5
    • Tacsimate 6 v/v pH 6
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 25 w/v -
    NA
    60 μl of Tacsimate pH 6 (100 v/v)
    100 μl of MES pH 6 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    D6
    • Magnesium formate 0.2 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 5,000 18 w/v -
    pH 4
    200 μl of Magnesium formate (1 M)
    100 μl of Sodium acetate pH 4 (1 M)
    360 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    340 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 2 v/v -
    • Imidazole 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 5,000 24 w/v -
    pH 7
    480 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of Imidazole pH 7 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    D8
    • Sodium formate 0.2 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 8.5
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    471 μl of water (100 v/v)
    E1
    • 2-Propanol 4 v/v -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    40 μl of 2-Propanol (100 v/v)
    460 μl of water (100 v/v)
    E2
    • Ethylene glycol 6 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 6,000 10 w/v -
    pH 3.5
    60 μl of Ethylene glycol (100 v/v)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    640 μl of water (100 v/v)
    E3
    • Lithium sulfate 0.15 M -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 6,000 18 w/v -
    pH 3.5
    360 μl of Polyethylene glycol 6,000 (50 w/v)
    75 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    465 μl of water (100 v/v)
    E4
    • 2-Propanol 10 v/v -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 6,000 22 w/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    440 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    360 μl of water (100 v/v)
    E5
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 8,000 22 w/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    40 μl of Sodium chloride (5 M)
    440 μl of Polyethylene glycol 8,000 (50 w/v)
    420 μl of water (100 v/v)
    E6
    • 2-Propanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 5 w/v -
    pH 8
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    E7
    • Polyethylene glycol 200 10 v/v -
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 8,000 18 w/v -
    pH 9
    100 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    440 μl of water (100 v/v)
    E8
    • 2-Propanol 15 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 10,000 10 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    150 μl of 2-Propanol (100 v/v)
    550 μl of water (100 v/v)
    F1
    • di-Sodium malonate 0.4 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 10,000 0.5 w/v -
    pH 6
    10 μl of Polyethylene glycol 10,000 (50 w/v)
    117 μl of di-Sodium malonate pH 6 (3.4 M)
    100 μl of MES pH 6 (1 M)
    772 μl of water (100 v/v)
    F2
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 6.5
    133 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    566 μl of water (100 v/v)
    F3
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    F4
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 10,000 16 w/v -
    pH 8
    200 μl of Ammonium acetate (1 M)
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    380 μl of water (100 v/v)
    F5
    • 2-Propanol 5 v/v -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 20,000 6 w/v -
    pH 3.5
    200 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of 2-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    650 μl of water (100 v/v)
    F6
    • di-Sodium malonate 1 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 20,000 2 w/v -
    pH 4.5
    294 μl of di-Sodium malonate pH 5 (3.4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    66 μl of Polyethylene glycol 20,000 (30 w/v)
    539 μl of water (100 v/v)
    F7
    • Magnesium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 20,000 10 w/v -
    pH 5
    100 μl of Magnesium chloride (2 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    466 μl of water (100 v/v)
    F8
    • Dextran sulfate sodium salt 5,000 3 w/v -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 20,000 15 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Dextran sulfate sodium salt 5,000 (30 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    300 μl of water (100 v/v)