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Supplemental Information

Screen Description

Pentaerythritol HTS

Pentaerythritol / Pentaerythritol HTS
Created 2009-11-16.

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Pentaerythritol HTS

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Pentaerythritol HTS

    Screen Structure

    Pentaerythritol HTS

    Well Count 96
    Number of distinct ingredients 11
    Internal diversity (score mean dist) 0.857

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:38:33.119780 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Pentaerythritol HTS
    Buffer 112
    Polymer 48
    Salt 72

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Pentaerythritol HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    483 μl of water (100 v/v)
    A2
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    316 μl of water (100 v/v)
    A3
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    150 μl of water (100 v/v)
    A4
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of MES pH 6.5 (1 M)
    483 μl of water (100 v/v)
    A5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of MES pH 6.5 (1 M)
    316 μl of water (100 v/v)
    A6
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of MES pH 6.5 (1 M)
    150 μl of water (100 v/v)
    A7
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    483 μl of water (100 v/v)
    A8
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    316 μl of water (100 v/v)
    A9
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of water (100 v/v)
    A10
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    483 μl of water (100 v/v)
    A11
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    316 μl of water (100 v/v)
    A12
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    B1
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.05 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    463 μl of water (100 v/v)
    B2
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    B3
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Potassium chloride 0.4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of Potassium chloride (4 M)
    50 μl of water (100 v/v)
    B4
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.05 M -
    		pH 6.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of MES pH 6.5 (1 M)
    463 μl of water (100 v/v)
    B5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of MES pH 6.5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    B6
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    • Potassium chloride 0.4 M -
    		pH 6.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of Potassium chloride (4 M)
    100 μl of MES pH 6.5 (1 M)
    50 μl of water (100 v/v)
    B7
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.05 M -
    		pH 7.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    463 μl of water (100 v/v)
    B8
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    		pH 7.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    B9
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    • Potassium chloride 0.4 M -
    		pH 7.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of Potassium chloride (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of water (100 v/v)
    B10
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.05 M -
    		pH 8.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    463 μl of water (100 v/v)
    B11
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 w/v -
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    583 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    B12
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.4 M -
    		pH 8.5
    750 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of Potassium chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of water (100 v/v)
    C1
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    483 μl of water (100 v/v)
    C2
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    316 μl of water (100 v/v)
    C3
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    150 μl of water (100 v/v)
    C4
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    483 μl of water (100 v/v)
    C5
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    316 μl of water (100 v/v)
    C6
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    150 μl of water (100 v/v)
    C7
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    483 μl of water (100 v/v)
    C8
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    316 μl of water (100 v/v)
    C9
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of water (100 v/v)
    C10
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    483 μl of water (100 v/v)
    C11
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    316 μl of water (100 v/v)
    C12
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    D1
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.05 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    463 μl of water (100 v/v)
    D2
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    250 μl of water (100 v/v)
    D3
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Potassium chloride 0.3 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    60 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    90 μl of water (100 v/v)
    D4
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.05 M -
    		pH 6.5
    20 μl of Magnesium chloride (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    463 μl of water (100 v/v)
    D5
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    250 μl of water (100 v/v)
    D6
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    • Potassium chloride 0.3 M -
    		pH 6.5
    60 μl of Potassium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    90 μl of water (100 v/v)
    D7
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.05 M -
    		pH 7.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    463 μl of water (100 v/v)
    D8
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    		pH 7.5
    66 μl of Ammonium sulfate (3 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    250 μl of water (100 v/v)
    D9
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    • Potassium chloride 0.3 M -
    		pH 7.5
    60 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    90 μl of water (100 v/v)
    D10
    • Pentaerythritol propoxylate (17/8 PO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.05 M -
    		pH 8.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    463 μl of water (100 v/v)
    D11
    • Pentaerythritol propoxylate (17/8 PO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.2 M -
    		pH 8.5
    66 μl of Ammonium sulfate (3 M)
    583 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of water (100 v/v)
    D12
    • Pentaerythritol propoxylate (17/8 PO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.3 M -
    		pH 8.5
    60 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol propoxylate (17/8 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    90 μl of water (100 v/v)
    E1
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    E2
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    E3
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    E4
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    E5
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    E6
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    E7
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    E8
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    E9
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    E10
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    E11
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    E12
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    F1
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.05 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    F2
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    250 μl of water (100 v/v)
    F3
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Potassium chloride 0.4 M -
    		pH 4.6
    100 μl of Potassium chloride (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    50 μl of water (100 v/v)
    F4
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.05 M -
    		pH 6.5
    20 μl of Magnesium chloride (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    F5
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    250 μl of water (100 v/v)
    F6
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    • Potassium chloride 0.4 M -
    		pH 6.5
    100 μl of Potassium chloride (4 M)
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    50 μl of water (100 v/v)
    F7
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.05 M -
    		pH 7.5
    20 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    F8
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    		pH 7.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    250 μl of water (100 v/v)
    F9
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    • Potassium chloride 0.4 M -
    		pH 7.5
    100 μl of Potassium chloride (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    50 μl of water (100 v/v)
    F10
    • Pentaerythritol ethoxylate (3/4 EO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.05 M -
    		pH 8.5
    20 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    F11
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.2 M -
    		pH 8.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    250 μl of water (100 v/v)
    F12
    • Pentaerythritol ethoxylate (3/4 EO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.4 M -
    		pH 8.5
    100 μl of Potassium chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (60 w/v)
    50 μl of water (100 v/v)
    G1
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    G2
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    G3
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    G4
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    483 μl of water (100 v/v)
    G5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    316 μl of water (100 v/v)
    G6
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    150 μl of water (100 v/v)
    G7
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    483 μl of water (100 v/v)
    G8
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    316 μl of water (100 v/v)
    G9
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of water (100 v/v)
    G10
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    483 μl of water (100 v/v)
    G11
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    316 μl of water (100 v/v)
    G12
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    H1
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.05 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    H2
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    H3
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Potassium chloride 0.4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    80 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    70 μl of water (100 v/v)
    H4
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.05 M -
    		pH 6.5
    20 μl of Magnesium chloride (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    463 μl of water (100 v/v)
    H5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    H6
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • MES 0.1 M pH 6.5
    • Potassium chloride 0.4 M -
    		pH 6.5
    80 μl of Potassium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    70 μl of water (100 v/v)
    H7
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.05 M -
    		pH 7.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    463 μl of water (100 v/v)
    H8
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    		pH 7.5
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    H9
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • HEPES 0.1 M pH 7.5
    • Potassium chloride 0.4 M -
    		pH 7.5
    80 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    70 μl of water (100 v/v)
    H10
    • Pentaerythritol ethoxylate (15/4 EO/OH) 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.05 M -
    		pH 8.5
    20 μl of Magnesium chloride (2.5 M)
    416 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    463 μl of water (100 v/v)
    H11
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.2 M -
    		pH 8.5
    583 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    116 μl of water (100 v/v)
    H12
    • Pentaerythritol ethoxylate (15/4 EO/OH) 45 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.4 M -
    		pH 8.5
    80 μl of Potassium chloride (5 M)
    750 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    70 μl of water (100 v/v)