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Supplemental Information

Screen Description

Cryo 1&2

Rigaku / Cryo 1&2
Created 2009-11-16.

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Cryo 1&2

    Screen Structure

    Cryo 1&2

    Well Count 96
    Number of distinct ingredients 34
    Internal diversity (score mean dist) 0.944

    Screen Composition

    2025-06-14T13:26:05.239411 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Cryo 1&2
    Buffer 105
    Cryoprotectant 17
    Organic (non-volatile) 63
    Organic (volatile) 9
    Polymer 59
    Salt 95

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Cryo 1&2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium phosphate citrate 0.1 M pH 4.2
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.5
    • Ethylene glycol 40 v/v -
    pH 4.5
    400 μl of Ethylene glycol (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    500 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 200 50 v/v -
    pH 5.5
    5 μl of tri-Sodium citrate pH 3 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    400 μl of water (100 v/v)
    A4
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 300 40 v/v -
    pH 7.5
    400 μl of Polyethylene glycol 300 (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    A5
    • Magnesium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    pH 5.5
    100 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1 M)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    400 μl of water (100 v/v)
    A6
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 600 40 v/v -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    400 μl of Polyethylene glycol 600 (100 v/v)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    A7
    • Magnesium chloride 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ethanol 40 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ethanol (100 v/v)
    25 μl of Magnesium chloride (2 M)
    475 μl of water (100 v/v)
    A8
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Ethoxyethanol 35 v/v -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    350 μl of 2-Ethoxyethanol (100 v/v)
    550 μl of water (100 v/v)
    A9
    • Sodium phosphate citrate 0.1 M pH 4.2
    • 2-Propanol 35 v/v -
    pH 4.2
    350 μl of 2-Propanol (100 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    550 μl of water (100 v/v)
    A10
    • Imidazole 0.1 M pH 8
    • Glycerol 45 v/v -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    450 μl of Glycerol (100 v/v)
    450 μl of water (100 v/v)
    A11
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    492 μl of water (100 v/v)
    A12
    • Sodium acetate 0.1 M pH 4.5
    • Ethylene glycol 50 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    pH 4.5
    500 μl of Ethylene glycol (100 v/v)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of water (100 v/v)
    B1
    • MES 0.1 M pH 6
    • Polyethylene glycol 3,000 5 w/v -
    • Polyethylene glycol 200 30 v/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    300 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of water (100 v/v)
    B2
    • Ammonium sulfate 0.2 M -
    • Glycerol 10 v/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 20 v/v -
    pH 4.2
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Glycerol (100 v/v)
    542 μl of water (100 v/v)
    B3
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 400 50 v/v -
    • CHES 0.1 M pH 9.5
    pH 9.5
    500 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of CHES pH 9.5 (1 M)
    360 μl of water (100 v/v)
    B4
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 v/v -
    • Glycerol 10 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 600 (100 v/v)
    300 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • 1,2-Propanediol 40 v/v -
    pH 7.5
    400 μl of 1,2-Propanediol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    B6
    • Imidazole 0.1 M pH 8
    • Calcium acetate 0.05 M -
    • 2-Ethoxyethanol 35 v/v -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    50 μl of Calcium acetate (1 M)
    350 μl of 2-Ethoxyethanol (100 v/v)
    500 μl of water (100 v/v)
    B7
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 35 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of 2-Propanol (100 v/v)
    550 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5.5
    • 1,2-Propanediol 30 v/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 5.5
    300 μl of 1,2-Propanediol (100 v/v)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1 M)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    400 μl of water (100 v/v)
    B9
    • Sodium acetate 0.1 M pH 4.5
    • 1,2-Propanediol 40 v/v -
    • Calcium acetate 0.05 M -
    pH 4.5
    400 μl of 1,2-Propanediol (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    50 μl of Calcium acetate (1 M)
    450 μl of water (100 v/v)
    B10
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Ethylene glycol 40 v/v -
    pH 6.2
    400 μl of Ethylene glycol (100 v/v)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    575 μl of water (100 v/v)
    B11
    • tris chloride BCC 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Ammonium sulfate 0.2 M -
    pH 7
    57 μl of Ammonium sulfate (3.5 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    442 μl of water (100 v/v)
    B12
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 400 40 v/v -
    • Sodium chloride 0.2 M -
    pH 6.2
    400 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Sodium chloride (5 M)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    535 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 200 30 v/v -
    • di-Ammonium hydrogen phosphate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    542 μl of water (100 v/v)
    C2
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 300 40 v/v -
    • Sodium chloride 0.2 M -
    pH 9.5
    400 μl of Polyethylene glycol 300 (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of CHES pH 9.5 (1 M)
    460 μl of water (100 v/v)
    C3
    • CAPS 0.1 M pH 10
    • Polyethylene glycol 400 30 v/v -
    • Glycerol 10 v/v -
    • Ammonium sulfate 0.5 M -
    pH 10
    142 μl of Ammonium sulfate (3.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Glycerol (100 v/v)
    100 μl of CAPS pH 10 (1 M)
    357 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 600 30 v/v -
    • Glycerol 10 v/v -
    • Lithium sulfate 0.05 M -
    pH 7.5
    100 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 600 (100 v/v)
    20 μl of Lithium sulfate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of water (100 v/v)
    C5
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 300 40 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 9.5
    400 μl of Polyethylene glycol 300 (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of CHES pH 9.5 (1 M)
    375 μl of water (100 v/v)
    C6
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-Ethoxyethanol 35 v/v -
    pH 5.5
    5 μl of tri-Sodium citrate pH 3 (1 M)
    350 μl of 2-Ethoxyethanol (100 v/v)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    550 μl of water (100 v/v)
    C7
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-Propanol 35 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    pH 5.5
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    350 μl of 2-Propanol (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1 M)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    450 μl of water (100 v/v)
    C8
    • CHES 0.1 M pH 9.5
    • 1,2-Propanediol 40 v/v -
    • tri-Sodium citrate 0.2 M -
    pH 9.5
    400 μl of 1,2-Propanediol (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    100 μl of CHES pH 9.5 (1 M)
    375 μl of water (100 v/v)
    C9
    • Imidazole 0.1 M pH 8
    • 1,2-Propanediol 25 v/v -
    • Zinc acetate 0.2 M -
    • Glycerol 10 v/v -
    pH 8
    250 μl of 1,2-Propanediol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    100 μl of Glycerol (100 v/v)
    200 μl of Zinc acetate (1 M)
    350 μl of water (100 v/v)
    C10
    • Imidazole 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Magnesium chloride 0.2 M -
    pH 8
    100 μl of Magnesium chloride (2 M)
    100 μl of Imidazole pH 8 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    C11
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 40 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    pH 7.5
    400 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    400 μl of water (100 v/v)
    C12
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 200 50 v/v -
    • Lithium sulfate 0.05 M -
    pH 7
    20 μl of Lithium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    380 μl of water (100 v/v)
    D1
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 300 40 v/v -
    • Calcium acetate 0.2 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    D2
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 40 v/v -
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    420 μl of water (100 v/v)
    D3
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 600 40 v/v -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 600 (100 v/v)
    500 μl of water (100 v/v)
    D4
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Ethanol 40 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    pH 4.2
    400 μl of Ethanol (100 v/v)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of water (100 v/v)
    D5
    • Sodium phosphate citrate 0.1 M pH 4.2
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    pH 4.2
    250 μl of 1,2-Propanediol (100 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Glycerol (100 v/v)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    450 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 7
    • Ethylene glycol 40 v/v -
    pH 7
    400 μl of Ethylene glycol (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of water (100 v/v)
    D7
    • tris chloride BCC 0.1 M pH 8.5
    • Ethylene glycol 50 v/v -
    • Magnesium chloride 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ethylene glycol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    D8
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 200 50 v/v -
    • Zinc acetate 0.2 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    200 μl of Zinc acetate (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    200 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 300 20 v/v -
    • Glycerol 10 v/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Glycerol (100 v/v)
    500 μl of water (100 v/v)
    D10
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 40 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    300 μl of water (100 v/v)
    D11
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 50 v/v -
    • Lithium sulfate 0.2 M -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    500 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    320 μl of water (100 v/v)
    D12
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 600 40 v/v -
    • Zinc acetate 0.2 M -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Polyethylene glycol 600 (100 v/v)
    200 μl of Zinc acetate (1 M)
    300 μl of water (100 v/v)
    E1
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    400 μl of water (100 v/v)
    E2
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 200 50 v/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of water (100 v/v)
    E3
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Ethylene glycol 40 v/v -
    • Ammonium sulfate 0.2 M -
    pH 4.2
    400 μl of Ethylene glycol (100 v/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    442 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 40 v/v -
    • Calcium acetate 0.2 M -
    pH 7.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    E5
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 300 40 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    pH 7
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of water (100 v/v)
    E6
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 600 30 v/v -
    • Glycerol 10 v/v -
    • Sodium chloride 1 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    100 μl of Glycerol (100 v/v)
    200 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    300 μl of water (100 v/v)
    E7
    • tris chloride BCC 0.1 M pH 7
    • Ethanol 40 v/v -
    pH 7
    400 μl of Ethanol (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of water (100 v/v)
    E8
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • 2-Ethoxyethanol 35 v/v -
    • Sodium chloride 0.2 M -
    pH 6.2
    350 μl of 2-Ethoxyethanol (100 v/v)
    40 μl of Sodium chloride (5 M)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    585 μl of water (100 v/v)
    E9
    • Imidazole 0.1 M pH 8
    • 2-Propanol 35 v/v -
    • Zinc acetate 0.05 M -
    pH 8
    350 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    50 μl of Zinc acetate (1 M)
    500 μl of water (100 v/v)
    E10
    • Sodium acetate 0.1 M pH 4.5
    • 1,2-Propanediol 40 v/v -
    pH 4.5
    400 μl of 1,2-Propanediol (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    500 μl of water (100 v/v)
    E11
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    pH 6.2
    250 μl of 1,2-Propanediol (100 v/v)
    100 μl of Glycerol (100 v/v)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    625 μl of water (100 v/v)
    E12
    • tri-Sodium citrate 0.1 M pH 5.5
    • 1,2-Propanediol 40 v/v -
    • Sodium chloride 0.2 M -
    pH 5.5
    400 μl of 1,2-Propanediol (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1 M)
    40 μl of Sodium chloride (5 M)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    460 μl of water (100 v/v)
    F1
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Zinc acetate 0.05 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    50 μl of Zinc acetate (1 M)
    500 μl of water (100 v/v)
    F2
    • Imidazole 0.1 M pH 8
    • Ethylene glycol 40 v/v -
    • Calcium acetate 0.2 M -
    pH 8
    400 μl of Ethylene glycol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    F3
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 200 50 v/v -
    • Sodium chloride 0.2 M -
    pH 6.2
    40 μl of Sodium chloride (5 M)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    435 μl of water (100 v/v)
    F4
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 300 20 v/v -
    • Glycerol 10 v/v -
    • Ammonium sulfate 1 M -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Glycerol (100 v/v)
    314 μl of water (100 v/v)
    F5
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 50 v/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    500 μl of Polyethylene glycol 400 (100 v/v)
    300 μl of water (100 v/v)
    F6
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    500 μl of water (100 v/v)
    F7
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 600 40 v/v -
    • Magnesium chloride 0.2 M -
    pH 4.5
    80 μl of Magnesium chloride pH 7 (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of Polyethylene glycol 600 (100 v/v)
    420 μl of water (100 v/v)
    F8
    • CHES 0.1 M pH 9.5
    • Ethylene glycol 50 v/v -
    • Potassium Sodium tartrate 0.5 M -
    pH 9.5
    500 μl of Ethylene glycol (100 v/v)
    333 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of CHES pH 9.5 (1 M)
    66 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Ethoxyethanol 35 v/v -
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of 2-Ethoxyethanol (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    470 μl of water (100 v/v)
    F10
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-Propanol 35 v/v -
    • Magnesium chloride 0.2 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    100 μl of Magnesium chloride (2 M)
    350 μl of 2-Propanol (100 v/v)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    450 μl of water (100 v/v)
    F11
    • HEPES 0.1 M pH 7.5
    • 1,2-Propanediol 30 v/v -
    • Polyethylene glycol 400 20 v/v -
    pH 7.5
    300 μl of 1,2-Propanediol (100 v/v)
    200 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 10 v/v -
    • 1,2-Propanediol 25 v/v -
    • Magnesium chloride 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of 1,2-Propanediol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of Glycerol (100 v/v)
    450 μl of water (100 v/v)
    G1
    • CAPS 0.1 M pH 10
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    pH 10
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of CAPS pH 10 (1 M)
    500 μl of water (100 v/v)
    G2
    • MES 0.1 M pH 6
    • Ethylene glycol 40 v/v -
    • Zinc acetate 0.2 M -
    pH 6
    400 μl of Ethylene glycol (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    200 μl of Zinc acetate (1 M)
    200 μl of water (100 v/v)
    G3
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 200 50 v/v -
    pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of water (100 v/v)
    G4
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 300 40 v/v -
    • Zinc acetate 0.2 M -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    200 μl of Zinc acetate (1 M)
    300 μl of water (100 v/v)
    G5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    • Glycerol 10 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Glycerol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    400 μl of water (100 v/v)
    G6
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 600 40 v/v -
    pH 5.5
    5 μl of tri-Sodium citrate pH 3 (1 M)
    400 μl of Polyethylene glycol 600 (100 v/v)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    500 μl of water (100 v/v)
    G7
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 600 40 v/v -
    pH 9.5
    400 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    G8
    • Sodium acetate 0.1 M pH 4.5
    • 2-Propanol 35 v/v -
    pH 4.5
    350 μl of 2-Propanol (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    550 μl of water (100 v/v)
    G9
    • Sodium cacodylate 0.1 M pH 6.5
    • Glycerol 45 v/v -
    • Calcium acetate 0.2 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    450 μl of Glycerol (100 v/v)
    200 μl of Calcium acetate (1 M)
    250 μl of water (100 v/v)
    G10
    • tris chloride BCC 0.1 M pH 7
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    • Ammonium sulfate 0.2 M -
    pH 7
    250 μl of 1,2-Propanediol (100 v/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    492 μl of water (100 v/v)
    G11
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    pH 5.5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1 M)
    94 μl of tri-Sodium citrate pH 6.2 (1 M)
    500 μl of water (100 v/v)
    G12
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 200 50 v/v -
    • Magnesium chloride 0.2 M -
    pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    100 μl of Magnesium chloride (2 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    300 μl of water (100 v/v)
    H1
    • Imidazole 0.1 M pH 8
    • Ethylene glycol 50 v/v -
    pH 8
    500 μl of Ethylene glycol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    400 μl of water (100 v/v)
    H2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 40 v/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    H3
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    • Glycerol 10 v/v -
    • Ammonium sulfate 0.5 M -
    pH 7
    142 μl of Ammonium sulfate (3.5 M)
    100 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    357 μl of water (100 v/v)
    H4
    • CHES 0.1 M pH 9.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    pH 9.5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    H5
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 50 v/v -
    • Lithium sulfate 0.2 M -
    pH 7.5
    500 μl of Ethylene glycol (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of water (100 v/v)
    H6
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 200 30 v/v -
    • Sodium chloride 0.1 M -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    580 μl of water (100 v/v)
    H7
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 400 40 v/v -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    H8
    • Sodium acetate 0.1 M pH 4.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Glycerol 10 v/v -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Glycerol (100 v/v)
    450 μl of water (100 v/v)
    H9
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 40 v/v -
    • Sodium chloride 0.2 M -
    pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    H10
    • CAPS 0.1 M pH 10
    • Polyethylene glycol 200 30 v/v -
    • Ammonium sulfate 0.2 M -
    pH 10
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of CAPS pH 10 (1 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    542 μl of water (100 v/v)
    H11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 200 50 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of water (100 v/v)
    H12
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 200 50 v/v -
    • Sodium chloride 0.2 M -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    40 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    360 μl of water (100 v/v)