Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Cryo 1&2

Rigaku / Cryo 1&2
Created 2009-11-16.

Export

Barcodes

Cryo 1&2

No barcodes found.

Screen Structure

Cryo 1&2

Well Count 96
Number of distinct ingredients 34
Internal diversity (score mean dist) 0.944

Screen Composition

2025-08-16T16:27:05.701196 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Cryo 1&2
Buffer 105
Cryoprotectant 17
Organic (non-volatile) 63
Organic (volatile) 9
Polymer 59
Salt 95

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Cryo 1&2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium phosphate citrate 0.1 M pH 4.2
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 4.2
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.5
  • Ethylene glycol 40 v/v -
pH 4.5
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
A3
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 200 50 v/v -
pH 5.5
500.0 μl of Polyethylene glycol 200 (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
400.0 μl of water (100 v/v)
A4
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 300 40 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
A5
  • Magnesium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
400.0 μl of water (100 v/v)
A6
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 600 40 v/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 600 (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
300.0 μl of water (100 v/v)
A7
  • Magnesium chloride 0.05 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ethanol 40 v/v -
pH 8.5
400.0 μl of Ethanol (100 v/v)
25.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
A8
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Ethoxyethanol 35 v/v -
pH 6.5
350.0 μl of 2-Ethoxyethanol (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
550.0 μl of water (100 v/v)
A9
  • Sodium phosphate citrate 0.1 M pH 4.2
  • 2-Propanol 35 v/v -
pH 4.2
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
550.0 μl of water (100 v/v)
A10
  • Imidazole 0.1 M pH 8
  • Glycerol 45 v/v -
pH 8
450.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
450.0 μl of water (100 v/v)
A11
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 8.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
492.9 μl of water (100 v/v)
A12
  • Sodium acetate 0.1 M pH 4.5
  • Ethylene glycol 50 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 4.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • MES 0.1 M pH 6
  • Polyethylene glycol 3,000 5 w/v -
  • Polyethylene glycol 200 30 v/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of water (100 v/v)
B2
  • Ammonium sulfate 0.2 M -
  • Glycerol 10 v/v -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 20 v/v -
pH 4.2
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
542.9 μl of water (100 v/v)
B3
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 400 50 v/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
B4
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 v/v -
  • Glycerol 10 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 6
100.0 μl of Glycerol (100 v/v)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
300.0 μl of Polyethylene glycol 600 (100 v/v)
300.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • 1,2-Propanediol 40 v/v -
pH 7.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of water (100 v/v)
B6
  • Imidazole 0.1 M pH 8
  • Calcium acetate 0.05 M -
  • 2-Ethoxyethanol 35 v/v -
pH 8
350.0 μl of 2-Ethoxyethanol (100 v/v)
50.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of water (100 v/v)
B7
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 35 v/v -
pH 8.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 5.5
  • 1,2-Propanediol 30 v/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5.5
300.0 μl of 1,2-Propanediol (100 v/v)
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
400.0 μl of water (100 v/v)
B9
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
  • Calcium acetate 0.05 M -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
50.0 μl of Calcium acetate (1 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
450.0 μl of water (100 v/v)
B10
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Ethylene glycol 40 v/v -
pH 6.2
400.0 μl of Ethylene glycol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
575.0 μl of water (100 v/v)
B11
  • tris chloride BCC 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Ammonium sulfate 0.2 M -
pH 7
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
442.9 μl of water (100 v/v)
B12
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 400 40 v/v -
  • Sodium chloride 0.2 M -
pH 6.2
400.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
40.0 μl of Sodium chloride (5 M)
535.0 μl of water (100 v/v)
C1
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 200 30 v/v -
  • di-Ammonium hydrogen phosphate 0.2 M -
pH 8.5
300.0 μl of Polyethylene glycol 200 (100 v/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
542.9 μl of water (100 v/v)
C2
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 300 40 v/v -
  • Sodium chloride 0.2 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
C3
  • CAPS 0.1 M pH 10
  • Polyethylene glycol 400 30 v/v -
  • Glycerol 10 v/v -
  • Ammonium sulfate 0.5 M -
pH 10
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
357.1 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 30 v/v -
  • Glycerol 10 v/v -
  • Lithium sulfate 0.05 M -
pH 7.5
100.0 μl of Glycerol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Lithium sulfate (2.5 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
480.0 μl of water (100 v/v)
C5
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 300 40 v/v -
  • tri-Sodium citrate 0.2 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
375.0 μl of water (100 v/v)
C6
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-Ethoxyethanol 35 v/v -
pH 5.5
350.0 μl of 2-Ethoxyethanol (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
550.0 μl of water (100 v/v)
C7
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-Propanol 35 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 5.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
450.0 μl of water (100 v/v)
C8
  • CHES 0.1 M pH 9.5
  • 1,2-Propanediol 40 v/v -
  • tri-Sodium citrate 0.2 M -
pH 9.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of CHES pH 9.5 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
375.0 μl of water (100 v/v)
C9
  • Imidazole 0.1 M pH 8
  • 1,2-Propanediol 25 v/v -
  • Zinc acetate 0.2 M -
  • Glycerol 10 v/v -
pH 8
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Zinc acetate (1 M)
350.0 μl of water (100 v/v)
C10
  • Imidazole 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Magnesium chloride 0.2 M -
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
100.0 μl of Magnesium chloride (2 M)
400.0 μl of water (100 v/v)
C11
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 7.5
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of water (100 v/v)
C12
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 200 50 v/v -
  • Lithium sulfate 0.05 M -
pH 7
20.0 μl of Lithium sulfate (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
380.0 μl of water (100 v/v)
D1
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
300.0 μl of water (100 v/v)
D2
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 40 v/v -
  • Lithium sulfate 0.2 M -
pH 8.5
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D3
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 600 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
D4
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Ethanol 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 4.2
400.0 μl of Ethanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
D5
  • Sodium phosphate citrate 0.1 M pH 4.2
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 4.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
450.0 μl of water (100 v/v)
D6
  • tris chloride BCC 0.1 M pH 7
  • Ethylene glycol 40 v/v -
pH 7
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
D7
  • tris chloride BCC 0.1 M pH 8.5
  • Ethylene glycol 50 v/v -
  • Magnesium chloride 0.2 M -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D8
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 200 50 v/v -
  • Zinc acetate 0.2 M -
pH 6.5
500.0 μl of Polyethylene glycol 200 (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
200.0 μl of Zinc acetate (1 M)
200.0 μl of water (100 v/v)
D9
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 300 20 v/v -
  • Glycerol 10 v/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 8.5
100.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
D10
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
D11
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 50 v/v -
  • Lithium sulfate 0.2 M -
pH 4.5
80.0 μl of Lithium sulfate (2.5 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
320.0 μl of water (100 v/v)
D12
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 600 40 v/v -
  • Zinc acetate 0.2 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 600 (100 v/v)
200.0 μl of Zinc acetate (1 M)
300.0 μl of water (100 v/v)
E1
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
400.0 μl of water (100 v/v)
E2
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 200 50 v/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of water (100 v/v)
E3
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Ethylene glycol 40 v/v -
  • Ammonium sulfate 0.2 M -
pH 4.2
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
442.9 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 40 v/v -
  • Calcium acetate 0.2 M -
pH 7.5
200.0 μl of Calcium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 300 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
pH 7
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
400.0 μl of water (100 v/v)
E6
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 600 30 v/v -
  • Glycerol 10 v/v -
  • Sodium chloride 1 M -
pH 6.5
100.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 600 (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
200.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
E7
  • tris chloride BCC 0.1 M pH 7
  • Ethanol 40 v/v -
pH 7
400.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
500.0 μl of water (100 v/v)
E8
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • 2-Ethoxyethanol 35 v/v -
  • Sodium chloride 0.2 M -
pH 6.2
350.0 μl of 2-Ethoxyethanol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
40.0 μl of Sodium chloride (5 M)
585.0 μl of water (100 v/v)
E9
  • Imidazole 0.1 M pH 8
  • 2-Propanol 35 v/v -
  • Zinc acetate 0.05 M -
pH 8
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
50.0 μl of Zinc acetate (1 M)
500.0 μl of water (100 v/v)
E10
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
E11
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
pH 6.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
625.0 μl of water (100 v/v)
E12
  • tri-Sodium citrate 0.1 M pH 5.5
  • 1,2-Propanediol 40 v/v -
  • Sodium chloride 0.2 M -
pH 5.5
400.0 μl of 1,2-Propanediol (100 v/v)
40.0 μl of Sodium chloride (5 M)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
460.0 μl of water (100 v/v)
F1
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Zinc acetate 0.05 M -
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
50.0 μl of Zinc acetate (1 M)
500.0 μl of water (100 v/v)
F2
  • Imidazole 0.1 M pH 8
  • Ethylene glycol 40 v/v -
  • Calcium acetate 0.2 M -
pH 8
200.0 μl of Calcium acetate (1 M)
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
300.0 μl of water (100 v/v)
F3
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
pH 6.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
40.0 μl of Sodium chloride (5 M)
435.0 μl of water (100 v/v)
F4
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 300 20 v/v -
  • Glycerol 10 v/v -
  • Ammonium sulfate 1 M -
pH 8
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 300 (100 v/v)
314.3 μl of water (100 v/v)
F5
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 50 v/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
F6
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
F7
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 600 40 v/v -
  • Magnesium chloride 0.2 M -
pH 4.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
400.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
420.0 μl of water (100 v/v)
F8
  • CHES 0.1 M pH 9.5
  • Ethylene glycol 50 v/v -
  • Potassium Sodium tartrate 0.5 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
500.0 μl of Ethylene glycol (100 v/v)
333.3 μl of Potassium Sodium tartrate (1.5 M)
66.7 μl of water (100 v/v)
F9
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Ethoxyethanol 35 v/v -
  • Lithium sulfate 0.2 M -
pH 8.5
350.0 μl of 2-Ethoxyethanol (100 v/v)
80.0 μl of Lithium sulfate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
470.0 μl of water (100 v/v)
F10
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 35 v/v -
  • Magnesium chloride 0.2 M -
pH 6.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
450.0 μl of water (100 v/v)
F11
  • HEPES 0.1 M pH 7.5
  • 1,2-Propanediol 30 v/v -
  • Polyethylene glycol 400 20 v/v -
pH 7.5
300.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
F12
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 10 v/v -
  • 1,2-Propanediol 25 v/v -
  • Magnesium chloride 0.2 M -
pH 8.5
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
450.0 μl of water (100 v/v)
G1
  • CAPS 0.1 M pH 10
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 10
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of CAPS pH 10 (1 M)
500.0 μl of water (100 v/v)
G2
  • MES 0.1 M pH 6
  • Ethylene glycol 40 v/v -
  • Zinc acetate 0.2 M -
pH 6
400.0 μl of Ethylene glycol (100 v/v)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
200.0 μl of Zinc acetate (1 M)
200.0 μl of water (100 v/v)
G3
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 200 50 v/v -
pH 7
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
400.0 μl of water (100 v/v)
G4
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 300 40 v/v -
  • Zinc acetate 0.2 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
200.0 μl of Zinc acetate (1 M)
300.0 μl of water (100 v/v)
G5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
  • Glycerol 10 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 7.5
100.0 μl of Glycerol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
G6
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 600 40 v/v -
pH 5.5
400.0 μl of Polyethylene glycol 600 (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
500.0 μl of water (100 v/v)
G7
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 600 40 v/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 600 (100 v/v)
500.0 μl of water (100 v/v)
G8
  • Sodium acetate 0.1 M pH 4.5
  • 2-Propanol 35 v/v -
pH 4.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
550.0 μl of water (100 v/v)
G9
  • Sodium cacodylate 0.1 M pH 6.5
  • Glycerol 45 v/v -
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
450.0 μl of Glycerol (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
250.0 μl of water (100 v/v)
G10
  • tris chloride BCC 0.1 M pH 7
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
  • Ammonium sulfate 0.2 M -
pH 7
250.0 μl of 1,2-Propanediol (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
492.9 μl of water (100 v/v)
G11
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 5.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
36.3 μl of tri-Sodium citrate pH 3 (1 M)
63.7 μl of tri-Sodium citrate pH 6.2 (1 M)
500.0 μl of water (100 v/v)
G12
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 200 50 v/v -
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
31.1 μl of Sodium cacodylate pH 5 (1 M)
68.9 μl of Sodium cacodylate pH 7.4 (1 M)
300.0 μl of water (100 v/v)
H1
  • Imidazole 0.1 M pH 8
  • Ethylene glycol 50 v/v -
pH 8
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of water (100 v/v)
H2
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 40 v/v -
pH 4.5
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
H3
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
  • Glycerol 10 v/v -
  • Ammonium sulfate 0.5 M -
pH 7
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
357.1 μl of water (100 v/v)
H4
  • CHES 0.1 M pH 9.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 9.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of CHES pH 9.5 (1 M)
500.0 μl of water (100 v/v)
H5
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 50 v/v -
  • Lithium sulfate 0.2 M -
pH 7.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Lithium sulfate (2.5 M)
320.0 μl of water (100 v/v)
H6
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 200 30 v/v -
  • Sodium chloride 0.1 M -
pH 4.5
300.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
20.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
H7
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 400 40 v/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
H8
  • Sodium acetate 0.1 M pH 4.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Glycerol 10 v/v -
pH 4.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
450.0 μl of water (100 v/v)
H9
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 300 40 v/v -
  • Sodium chloride 0.2 M -
pH 4.5
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
H10
  • CAPS 0.1 M pH 10
  • Polyethylene glycol 200 30 v/v -
  • Ammonium sulfate 0.2 M -
pH 10
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10 (1 M)
300.0 μl of Polyethylene glycol 200 (100 v/v)
542.9 μl of water (100 v/v)
H11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 200 50 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of water (100 v/v)
H12
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
pH 4.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
360.0 μl of water (100 v/v)