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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG+
Barcodes
JCSG+
Screen Structure
JCSG+
Well Count | 96 |
---|---|
Number of distinct ingredients | 64 |
Internal diversity (score mean dist) | 0.958 |
Screen Composition
ingredient type | JCSG+ |
---|---|
Buffer | 96 |
Cryoprotectant | 6 |
Organic (non-volatile) | 25 |
Organic (volatile) | 6 |
Polymer | 62 |
Salt | 94 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 500 μl of Polyethylene glycol 400 (100 v/v) 50 μl of Sodium acetate pH 4.5 (2 M) 66 μl of Lithium sulfate (3 M) 383 μl of water (100 v/v) |
A2 |
| pH 5.5 | 400 μl of Polyethylene glycol 3,000 (50 w/v) 25 μl of tri-Sodium citrate pH 5.5 (4 M) 575 μl of water (100 v/v) |
A3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of di-Ammonium hydrogen citrate (2.5 M) 520 μl of water (100 v/v) |
A4 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Calcium chloride (1 M) 400 μl of water (100 v/v) |
A5 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium formate (1 M) 400 μl of water (100 v/v) |
A6 |
| pH 4.2 | 400 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tri-Sodium citrate pH 4.2 (1 M) 66 μl of Lithium sulfate (3 M) 433 μl of water (100 v/v) |
A7 |
| pH 9.5 | 100 μl of CHES pH 9.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v) |
A8 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of Ammonium formate (2.5 M) 520 μl of water (100 v/v) |
A9 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium chloride (5 M) 560 μl of water (100 v/v) |
A10 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 14 μl of Potassium formate (14 M) 585 μl of water (100 v/v) |
A11 |
| pH 8.5 | 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 57 μl of di-Ammonium hydrogen phosphate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 342 μl of water (100 v/v) |
A12 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Potassium nitrate (1 M) 400 μl of water (100 v/v) |
B1 |
| pH 4 | 66 μl of Ammonium sulfate (3 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 833 μl of water (100 v/v) |
B2 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 25 μl of Sodium thiocyanate (8 M) 575 μl of water (100 v/v) |
B3 |
| pH 9 | 100 μl of BICINE pH 9 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 500 μl of water (100 v/v) |
B4 |
| pH 7.5 | 80 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v) |
B5 |
| pH 6.5 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 400 μl of water (100 v/v) |
B6 |
| pH 4.2 | 100 μl of Polyethylene glycol 1,000 (50 w/v) 800 μl of Ethanol (50 v/v) 100 μl of tri-Sodium citrate pH 4.2 (1 M) |
B7 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v) |
B8 |
| pH 7 | 33 μl of tris chloride BCC pH 7 (3 M) 200 μl of Magnesium chloride pH 7 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 566 μl of water (100 v/v) |
B9 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 500 μl of water (100 v/v) |
B10 |
| pH 6.5 | 200 μl of Magnesium chloride pH 7 (1 M) 500 μl of Polyethylene glycol 200 (100 v/v) 100 μl of MES pH 6.5 (1 M) 200 μl of water (100 v/v) |
B11 |
| NA | 400 μl of tri-Sodium citrate (4 M) 600 μl of water (100 v/v) |
B12 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of tri-Potassium citrate (2.5 M) 520 μl of water (100 v/v) |
C1 |
| pH 4.2 | 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Sodium chloride (1 M) 300 μl of water (100 v/v) |
C2 |
| pH 4 | 200 μl of Lithium chloride (5 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 4 (1 M) 300 μl of water (100 v/v) |
C3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium nitrate (10 M) 580 μl of water (100 v/v) |
C4 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
C5 |
| pH 7.5 | 160 μl of di-Sodium hydrogen phosphate (5 M) 160 μl of di-Potassium hydrogen phosphate (5 M) 100 μl of HEPES pH 7.5 (1 M) 580 μl of water (100 v/v) |
C6 |
| pH 4.2 | 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 300 μl of Polyethylene glycol 300 (100 v/v) 600 μl of water (100 v/v) |
C7 |
| pH 4.6 | 100 μl of Zinc acetate (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 600 μl of water (100 v/v) |
C8 |
| pH 8.5 | 400 μl of Ethanol (50 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v) |
C9 |
| pH 6.2 | 100 μl of Glycerol (100 v/v) 250 μl of 1,2-Propanediol (100 v/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 625 μl of water (100 v/v) |
C10 |
| pH 9 | 40 μl of 1,4-Dioxane (50 v/v) 200 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 660 μl of water (100 v/v) |
C11 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 666 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v) |
C12 |
| NA | 200 μl of Polyethylene glycol 1,000 (50 w/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v) |
D1 |
| NA | 200 μl of Glycerol (100 v/v) 480 μl of Polyethylene glycol 1,500 (50 w/v) 320 μl of water (100 v/v) |
D2 |
| pH 7.5 | 300 μl of Polyethylene glycol 400 (100 v/v) 80 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 520 μl of water (100 v/v) |
D3 |
| pH 6.2 | 200 μl of Sodium chloride (1 M) 500 μl of Polyethylene glycol 200 (100 v/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 275 μl of water (100 v/v) |
D4 |
| pH 4.5 | 33 μl of Sodium acetate pH 4.5 (3 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 300 μl of water (100 v/v) |
D5 |
| pH 7.5 | 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v) |
D6 |
| pH 8.5 | 80 μl of Magnesium chloride pH 7 (2.5 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 420 μl of water (100 v/v) |
D7 |
| pH 8.5 | 400 μl of Polyethylene glycol 400 (100 v/v) 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 433 μl of water (100 v/v) |
D8 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500 μl of water (100 v/v) |
D9 |
| NA | 150 μl of Glycerol (100 v/v) 56 μl of Ammonium sulfate (3 M) 510 μl of Polyethylene glycol 4,000 (50 w/v) 283 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100 μl of Calcium acetate (2 M) 100 μl of MES pH 6.5 (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 400 μl of water (100 v/v) |
D11 |
| pH 4.6 | 70 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Glycerol (100 v/v) 140 μl of 2-Propanol (100 v/v) 140 μl of Calcium chloride (1 M) 350 μl of water (100 v/v) |
D12 |
| NA | 200 μl of Glycerol (100 v/v) 320 μl of Polyethylene glycol 8,000 (50 w/v) 8 μl of di-Potassium hydrogen phosphate (5 M) 472 μl of water (100 v/v) |
E1 |
| pH 6.5 | 250 μl of tri-Sodium citrate (4 M) 100 μl of MES pH 6.5 (1 M) 650 μl of water (100 v/v) |
E2 |
| pH 6.5 | 666 μl of Ammonium sulfate (3 M) 200 μl of Sodium chloride (1 M) 100 μl of MES pH 6.5 (1 M) 33 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Sodium chloride (1 M) 600 μl of water (100 v/v) |
E4 |
| pH 8.5 | 420 μl of Ammonium sulfate (3 M) 66 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 413 μl of water (100 v/v) |
E5 |
| pH 10 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of CAPS pH 10 (1 M) 500 μl of water (100 v/v) |
E6 |
| pH 8 | 100 μl of Imidazole pH 8 (1 M) 100 μl of Zinc acetate (2 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 400 μl of water (100 v/v) |
E7 |
| pH 6.5 | 100 μl of Zinc acetate (2 M) 100 μl of 2-Propanol (100 v/v) 100 μl of MES pH 6.5 (1 M) 700 μl of water (100 v/v) |
E8 |
| pH 4.5 | 33 μl of Sodium acetate pH 4.5 (3 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 681 μl of water (100 v/v) |
E9 |
| pH 6.5 | 800 μl of Magnesium sulfate (2 M) 100 μl of MES pH 6.5 (1 M) 100 μl of water (100 v/v) |
E10 |
| pH 9 | 100 μl of BICINE pH 9 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
E11 |
| pH 6.5 | 80 μl of Calcium acetate (2 M) 200 μl of Glycerol (100 v/v) 288 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of MES pH 6.5 (1 M) 352 μl of water (100 v/v) |
E12 |
| pH 8 | 100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v) |
F1 |
| pH 6.5 | 50 μl of Cesium chloride (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of MES pH 6.5 (1 M) 250 μl of water (100 v/v) |
F2 |
| pH 5 | 100 μl of tri-Sodium citrate pH 5 (1 M) 630 μl of Ammonium sulfate (5 M) 270 μl of water (100 v/v) |
F3 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 700 μl of water (100 v/v) |
F4 |
| pH 7.5 | 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
F5 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 500 μl of Ethylene glycol (100 v/v) 80 μl of Magnesium chloride pH 7 (2.5 M) 320 μl of water (100 v/v) |
F6 |
| pH 9 | 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BICINE pH 9 (1 M) 800 μl of water (100 v/v) |
F7 |
| NA | 266 μl of Cadmium chloride (3 M) 733 μl of water (100 v/v) |
F8 |
| NA | 700 μl of Cadmium chloride (3 M) 300 μl of water (100 v/v) |
F9 |
| NA | 800 μl of di-Sodium malonate (3 M) 200 μl of water (100 v/v) |
F10 |
| pH 7 | 366 μl of di-Sodium malonate (3 M) 10 μl of Cadmium chloride (50 w/v) 100 μl of HEPES pH 7 (1 M) 523 μl of water (100 v/v) |
F11 |
| pH 7 | 20 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 880 μl of water (100 v/v) |
F12 |
| pH 7 | 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7 (1 M) 300 μl of water (100 v/v) |
G1 |
| pH 7 | 600 μl of Cadmium chloride (50 w/v) 100 μl of HEPES pH 7 (1 M) 300 μl of water (100 v/v) |
G2 |
| pH 7.5 | 200 μl of Magnesium chloride pH 7 (1 M) 100 μl of HEPES pH 7.5 (1 M) 440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 260 μl of water (100 v/v) |
G3 |
| pH 8.5 | 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 100 μl of Cobalt (II) chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
G4 |
| pH 8.5 | 400 μl of Polyethylene glycol 1,000 (50 w/v) 200 μl of Trimethylamine N-oxide (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
G5 |
| pH 7.5 | 50 μl of Cadmium chloride (1 M) 240 μl of Polyethylene glycol 3,350 (50 w/v) 10 μl of Magnesium chloride (500 mM) 100 μl of HEPES pH 7.5 (1 M) 5 μl of Nickel (II) chloride pH 7 (1 M) 50 μl of Cobalt (II) chloride (1 M) 545 μl of water (100 v/v) |
G6 |
| pH 7.5 | 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of di-Sodium malonate (2 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v) |
G7 |
| NA | 300 μl of Polyethylene glycol 3,350 (50 w/v) 33 μl of Cadmium chloride (3 M) 666 μl of water (100 v/v) |
G8 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Cadmium chloride (3 M) 550 μl of water (100 v/v) |
G9 |
| NA | 600 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
G10 |
| NA | 600 μl of Polyethylene glycol 1,000 (50 w/v) 12 μl of Potassium thiocyanate (8 M) 387 μl of water (100 v/v) |
G11 |
| pH 5.5 | 666 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 233 μl of water (100 v/v) |
G12 |
| pH 5.5 | 600 μl of Sodium chloride (5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of water (100 v/v) |
H1 |
| pH 5.5 | 300 μl of Magnesium formate (1 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 600 μl of water (100 v/v) |
H2 |
| pH 5.5 | 20 μl of Polyethylene glycol 3,350 (50 w/v) 333 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 546 μl of water (100 v/v) |
H3 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 400 μl of water (100 v/v) |
H4 |
| pH 5.5 | 200 μl of Cadmium chloride (1 M) 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 450 μl of water (100 v/v) |
H5 |
| pH 5.5 | 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 40 μl of Ammonium acetate (5 M) 610 μl of water (100 v/v) |
H6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 20 μl of Ammonium acetate (5 M) 880 μl of water (100 v/v) |
H7 |
| pH 5.5 | 66 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 493 μl of water (100 v/v) |
H8 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Sodium chloride (1 M) 200 μl of water (100 v/v) |
H9 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 66 μl of Lithium sulfate (3 M) 333 μl of water (100 v/v) |
H10 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 40 μl of Ammonium acetate (5 M) 360 μl of water (100 v/v) |
H11 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 320 μl of water (100 v/v) |
H12 |
| pH 7.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 40 μl of Ammonium acetate (5 M) 410 μl of water (100 v/v) |