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Supplemental Information

Screen Description

pHAT

Rigaku / pHAT
Created 2009-11-16.

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pHAT

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pHAT

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Screen Structure

pHAT

Well Count 96
Number of distinct ingredients 12
Internal diversity (score mean dist) 0.926

Screen Composition

More information about screen composition chart.
2025-08-16T09:05:38.461432 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type pHAT
Buffer 96
Salt 16

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
pHAT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium lactate 1 M pH 2.4
pH 2.4
1000.0 μl of Sodium lactate pH 2.4 (1 M)
A2
  • Sodium acetate 1 M pH 3.4
pH 3.4
1000.0 μl of Sodium acetate pH 3.4 (1 M)
A3
  • MES 1 M pH 4.6
pH 4.6
1000.0 μl of MES pH 4.6 (1 M)
A4
  • BIS-TRIS 1 M pH 5.2
pH 5.2
1000.0 μl of BIS-TRIS pH 5.2 (1 M)
A5
  • Imidazole 1 M pH 5.4
pH 5.4
1000.0 μl of Imidazole pH 5.4 (1 M)
A6
  • Sodium Potassium phosphate 1 M pH 5.8
pH 5.8
253.9 μl of Sodium Potassium phosphate pH 5 (3 M)
79.4 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
A7
  • HEPES 1 M pH 6
pH 6
1000.0 μl of HEPES pH 6 (1 M)
A8
  • tris chloride BCC 1 M pH 6.6
pH 6.6
1000.0 μl of tris chloride BCC pH 6.6 (1 M)
A9
  • BICINE 1 M pH 7
pH 7
1000.0 μl of BICINE pH 7 (1 M)
A10
  • CHES 0.5 M pH 8
pH 8
500.0 μl of CHES pH 8 (1 M)
500.0 μl of water (100 v/v)
A11
  • Glycine 1 M pH 8.2
pH 8.2
500.0 μl of Glycine pH 8.2 (2 M)
500.0 μl of water (100 v/v)
A12
  • CAPS 0.5 M pH 8.8
pH 8.8
500.0 μl of CAPS pH 8.8 (1 M)
500.0 μl of water (100 v/v)
B1
  • Sodium lactate 1 M pH 2.8
pH 2.8
953.4 μl of Sodium lactate pH 2.4 (1 M)
46.6 μl of Sodium lactate pH 5.2 (1 M)
B2
  • Sodium acetate 1 M pH 3.8
pH 3.8
822.0 μl of Sodium acetate pH 3.4 (1 M)
178.0 μl of Sodium acetate pH 4.5 (1 M)
B3
  • MES 1 M pH 5
pH 5
937.3 μl of MES pH 4.6 (1 M)
62.7 μl of MES pH 6.5 (1 M)
B4
  • BIS-TRIS 1 M pH 5.6
pH 5.6
968.7 μl of BIS-TRIS pH 5.5 (1 M)
31.3 μl of BIS-TRIS pH 7 (1 M)
B5
  • Imidazole 1 M pH 5.8
pH 5.8
689.7 μl of Imidazole pH 5.4 (1 M)
310.3 μl of Imidazole pH 6.2 (1 M)
B6
  • Sodium Potassium phosphate 1 M pH 6.2
pH 6.2
205.1 μl of Sodium Potassium phosphate pH 5 (3 M)
128.3 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
B7
  • HEPES 1 M pH 6.4
pH 6.4
471.0 μl of HEPES pH 6 (1 M)
529.0 μl of HEPES pH 6.6 (1 M)
B8
  • tris chloride BCC 1 M pH 7
pH 7
1000.0 μl of tris chloride BCC pH 7 (1 M)
B9
  • BICINE 1 M pH 7.4
pH 7.4
1000.0 μl of BICINE pH 7.4 (1 M)
B10
  • CHES 0.5 M pH 8.4
pH 8.4
143.7 μl of CHES pH 8 (1 M)
356.3 μl of CHES pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B11
  • Glycine 1 M pH 8.6
pH 8.6
481.0 μl of Glycine pH 8.2 (2 M)
19.0 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
B12
  • CAPS 0.5 M pH 9.2
pH 9.2
302.6 μl of CAPS pH 8.8 (1 M)
197.4 μl of CAPS pH 9.5 (1 M)
500.0 μl of water (100 v/v)
C1
  • Sodium lactate 1 M pH 3.2
pH 3.2
852.8 μl of Sodium lactate pH 2.4 (1 M)
147.2 μl of Sodium lactate pH 5.2 (1 M)
C2
  • Sodium acetate 1 M pH 4.2
pH 4.2
450.3 μl of Sodium acetate pH 3.4 (1 M)
549.7 μl of Sodium acetate pH 4.5 (1 M)
C3
  • MES 1 M pH 5.4
pH 5.4
801.8 μl of MES pH 4.6 (1 M)
198.2 μl of MES pH 6.5 (1 M)
C4
  • BIS-TRIS 1 M pH 6
pH 6
1000.0 μl of BIS-TRIS pH 6 (1 M)
C5
  • Imidazole 1 M pH 6.2
pH 6.2
1000.0 μl of Imidazole pH 6.2 (1 M)
C6
  • Sodium Potassium phosphate 1 M pH 6.6
pH 6.6
142.8 μl of Sodium Potassium phosphate pH 5 (3 M)
190.5 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
C7
  • HEPES 1 M pH 6.8
pH 6.8
575.5 μl of HEPES pH 6.6 (1 M)
424.5 μl of HEPES pH 7 (1 M)
C8
  • tris chloride BCC 1 M pH 7.4
pH 7.4
748.7 μl of tris chloride BCC pH 7 (1 M)
251.3 μl of tris chloride BCC pH 8 (1 M)
C9
  • BICINE 1 M pH 7.8
pH 7.8
822.1 μl of BICINE pH 7.4 (1 M)
177.9 μl of BICINE pH 9 (1 M)
C10
  • CHES 0.5 M pH 8.8
pH 8.8
407.8 μl of CHES pH 8.5 (1 M)
92.2 μl of CHES pH 9.5 (1 M)
500.0 μl of water (100 v/v)
C11
  • Glycine 1 M pH 9
pH 9
438.7 μl of Glycine pH 8.2 (2 M)
61.3 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
C12
  • CAPS 0.5 M pH 9.6
pH 9.6
474.4 μl of CAPS pH 9.5 (1 M)
25.6 μl of CAPS pH 10 (1 M)
500.0 μl of water (100 v/v)
D1
  • Sodium lactate 1 M pH 3.6
pH 3.6
671.0 μl of Sodium lactate pH 2.4 (1 M)
329.0 μl of Sodium lactate pH 5.2 (1 M)
D2
  • Sodium acetate 1 M pH 4.6
pH 4.6
1000.0 μl of Sodium acetate pH 4.6 (1 M)
D3
  • MES 1 M pH 5.8
pH 5.8
557.1 μl of MES pH 4.6 (1 M)
442.9 μl of MES pH 6.5 (1 M)
D4
  • BIS-TRIS 1 M pH 6.4
pH 6.4
610.3 μl of BIS-TRIS pH 6 (1 M)
389.7 μl of BIS-TRIS pH 7 (1 M)
D5
  • Imidazole 1 M pH 6.6
pH 6.6
808.4 μl of Imidazole pH 6.2 (1 M)
191.6 μl of Imidazole pH 8 (1 M)
D6
  • Sodium Potassium phosphate 1 M pH 7
pH 7
90.9 μl of Sodium Potassium phosphate pH 5 (3 M)
242.5 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
D7
  • HEPES 1 M pH 7.2
pH 7.2
428.0 μl of HEPES pH 6.6 (1 M)
572.0 μl of HEPES pH 7.5 (1 M)
D8
  • tris chloride BCC 1 M pH 7.8
pH 7.8
294.8 μl of tris chloride BCC pH 7 (1 M)
705.2 μl of tris chloride BCC pH 8 (1 M)
D9
  • BICINE 1 M pH 8.2
pH 8.2
541.6 μl of BICINE pH 7.4 (1 M)
458.4 μl of BICINE pH 9 (1 M)
D10
  • CHES 0.5 M pH 9.2
pH 9.2
203.1 μl of CHES pH 8.5 (1 M)
296.9 μl of CHES pH 9.5 (1 M)
500.0 μl of water (100 v/v)
D11
  • Glycine 1 M pH 9.4
pH 9.4
358.0 μl of Glycine pH 8.2 (2 M)
142.0 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
D12
  • CAPS 0.5 M pH 10
pH 10
317.5 μl of CAPS pH 9.5 (1 M)
182.5 μl of CAPS pH 10 (1 M)
500.0 μl of water (100 v/v)
E1
  • Sodium lactate 1 M pH 4
pH 4
428.6 μl of Sodium lactate pH 2.4 (1 M)
571.4 μl of Sodium lactate pH 5.2 (1 M)
E2
  • Sodium acetate 1 M pH 5
pH 5
493.6 μl of Sodium acetate pH 4.6 (1 M)
506.4 μl of Sodium acetate pH 5.5 (1 M)
E3
  • MES 1 M pH 6.2
pH 6.2
230.7 μl of MES pH 4.6 (1 M)
769.3 μl of MES pH 6.5 (1 M)
E4
  • BIS-TRIS 1 M pH 6.8
pH 6.8
360.4 μl of BIS-TRIS pH 6.5 (1 M)
639.6 μl of BIS-TRIS pH 7 (1 M)
E5
  • Imidazole 1 M pH 7
pH 7
529.7 μl of Imidazole pH 6.2 (1 M)
470.3 μl of Imidazole pH 8 (1 M)
E6
  • Sodium Potassium phosphate 1 M pH 7.4
pH 7.4
48.4 μl of Sodium Potassium phosphate pH 5 (3 M)
284.9 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
E7
  • HEPES 1 M pH 7.6
pH 7.6
441.2 μl of HEPES pH 6.6 (1 M)
558.8 μl of HEPES pH 8.5 (1 M)
E8
  • tris chloride BCC 1 M pH 8.2
pH 8.2
246.1 μl of tris chloride BCC pH 7 (1 M)
753.9 μl of tris chloride BCC pH 8.5 (1 M)
E9
  • BICINE 1 M pH 8.6
pH 8.6
232.1 μl of BICINE pH 7.4 (1 M)
767.9 μl of BICINE pH 9 (1 M)
E10
  • CHES 0.5 M pH 9.6
pH 9.6
215.0 μl of CHES pH 8.5 (1 M)
285.0 μl of CHES pH 10 (1 M)
500.0 μl of water (100 v/v)
E11
  • Glycine 1 M pH 9.8
pH 9.8
240.5 μl of Glycine pH 8.2 (2 M)
259.5 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
E12
  • CAPS 0.5 M pH 10
pH 10
70.0 μl of CAPS pH 9.5 (1 M)
430.0 μl of CAPS pH 10 (1 M)
500.0 μl of water (100 v/v)
F1
  • Sodium lactate 1 M pH 4.4
pH 4.4
208.9 μl of Sodium lactate pH 2.4 (1 M)
791.1 μl of Sodium lactate pH 5.2 (1 M)
F2
  • Sodium acetate 1 M pH 5.4
pH 5.4
77.0 μl of Sodium acetate pH 4.6 (1 M)
923.0 μl of Sodium acetate pH 5.5 (1 M)
F3
  • MES 1 M pH 6.6
pH 6.6
812.3 μl of MES pH 6.5 (1 M)
187.7 μl of MES pH 7.4 (1 M)
F4
  • BIS-TRIS 1 M pH 7.2
pH 7.2
647.3 μl of BIS-TRIS pH 7 (1 M)
352.7 μl of BIS-TRIS pH 8 (1 M)
F5
  • Imidazole 1 M pH 7.4
pH 7.4
251.0 μl of Imidazole pH 6.2 (1 M)
749.0 μl of Imidazole pH 8 (1 M)
F6
  • Sodium Potassium phosphate 1 M pH 7.8
pH 7.8
24.7 μl of Sodium Potassium phosphate pH 5 (3 M)
308.7 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
F7
  • HEPES 1 M pH 8
pH 8
187.3 μl of HEPES pH 6.6 (1 M)
812.7 μl of HEPES pH 8.5 (1 M)
F8
  • tris chloride BCC 1 M pH 8.6
pH 8.6
157.7 μl of tris chloride BCC pH 7 (1 M)
842.3 μl of tris chloride BCC pH 9 (1 M)
F9
  • BICINE 1 M pH 9
pH 9
1000.0 μl of BICINE pH 9 (1 M)
F10
  • CHES 0.5 M pH 10
pH 10
91.3 μl of CHES pH 8.5 (1 M)
408.7 μl of CHES pH 10 (1 M)
500.0 μl of water (100 v/v)
F11
  • Glycine 1 M pH 10
pH 10
123.0 μl of Glycine pH 8.2 (2 M)
377.0 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
F12
  • CAPS 0.5 M pH 11
pH 11
148.5 μl of CAPS pH 9.5 (1 M)
351.5 μl of CAPS pH 12 (1 M)
500.0 μl of water (100 v/v)
G1
  • Sodium lactate 1 M pH 4.8
pH 4.8
69.5 μl of Sodium lactate pH 2.4 (1 M)
930.5 μl of Sodium lactate pH 5.2 (1 M)
G2
  • Sodium acetate 1 M pH 5.8
pH 5.8
411.0 μl of Sodium acetate pH 5.5 (1 M)
589.0 μl of Sodium acetate pH 6.2 (1 M)
G3
  • MES 1 M pH 7
pH 7
270.4 μl of MES pH 6.5 (1 M)
729.6 μl of MES pH 7.4 (1 M)
G4
  • BIS-TRIS 1 M pH 7.6
pH 7.6
204.8 μl of BIS-TRIS pH 7 (1 M)
795.2 μl of BIS-TRIS pH 8 (1 M)
G5
  • Imidazole 1 M pH 7.8
pH 7.8
59.4 μl of Imidazole pH 6.2 (1 M)
940.6 μl of Imidazole pH 8 (1 M)
G6
  • Sodium Potassium phosphate 1 M pH 8.2
pH 8.2
11.3 μl of Sodium Potassium phosphate pH 5 (3 M)
322.1 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
G7
  • HEPES 1 M pH 8.4
pH 8.4
26.2 μl of HEPES pH 6.6 (1 M)
973.8 μl of HEPES pH 8.5 (1 M)
G8
  • tris chloride BCC 1 M pH 9
pH 9
1000.0 μl of tris chloride BCC pH 9 (1 M)
G9
  • BICINE 1 M pH 9.4
pH 9.4
50.1 μl of BICINE pH 7.4 (1 M)
949.9 μl of BICINE pH 9.8 (1 M)
G10
  • CHES 0.5 M pH 10
pH 10
12.8 μl of CHES pH 8.5 (1 M)
487.2 μl of CHES pH 10 (1 M)
500.0 μl of water (100 v/v)
G11
  • Glycine 1 M pH 11
pH 11
42.3 μl of Glycine pH 8.2 (2 M)
457.7 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
G12
  • CAPS 0.5 M pH 11
pH 11
46.1 μl of CAPS pH 9.5 (1 M)
453.9 μl of CAPS pH 12 (1 M)
500.0 μl of water (100 v/v)
H1
  • Sodium lactate 1 M pH 5.2
pH 5.2
1000.0 μl of Sodium lactate pH 5.2 (1 M)
H2
  • Sodium acetate 1 M pH 6.2
pH 6.2
1000.0 μl of Sodium acetate pH 6.2 (1 M)
H3
  • MES 1 M pH 7.4
pH 7.4
1000.0 μl of MES pH 7.4 (1 M)
H4
  • BIS-TRIS 1 M pH 8
pH 8
1000.0 μl of BIS-TRIS pH 8 (1 M)
H5
  • Imidazole 1 M pH 8.2
pH 8.2
1000.0 μl of Imidazole pH 8.2 (1 M)
H6
  • Sodium Potassium phosphate 1 M pH 8.6
pH 8.6
333.3 μl of Sodium Potassium phosphate pH 8.6 (3 M)
666.7 μl of water (100 v/v)
H7
  • HEPES 1 M pH 8.8
pH 8.8
1000.0 μl of HEPES pH 8.8 (1 M)
H8
  • tris chloride BCC 1 M pH 9.4
pH 9.4
1000.0 μl of tris chloride BCC pH 9.4 (1 M)
H9
  • BICINE 1 M pH 9.8
pH 9.8
1000.0 μl of BICINE pH 9.8 (1 M)
H10
  • CHES 0.5 M pH 11
pH 11
500.0 μl of CHES pH 11 (1 M)
500.0 μl of water (100 v/v)
H11
  • Glycine 1 M pH 11
pH 11
500.0 μl of Glycine pH 11 (2 M)
500.0 μl of water (100 v/v)
H12
  • CAPS 0.5 M pH 12
pH 12
500.0 μl of CAPS pH 12 (1 M)
500.0 μl of water (100 v/v)