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Supplemental Information

Screen Description

Precipitant Synergy 33%

Rigaku / Precipitant Synergy 33%
Created 2009-11-16.

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Precipitant Synergy 33%

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Precipitant Synergy 33%

    Screen Structure

    Precipitant Synergy 33%

    Well Count 96
    Number of distinct ingredients 23
    Internal diversity (score mean dist) 0.894

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:51:51.628411 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Precipitant Synergy 33%
    Buffer 66
    Cryoprotectant 2
    Organic (non-volatile) 38
    Organic (volatile) 18
    Polymer 57
    Salt 73

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Precipitant Synergy 33% Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium sulfate 0.66 M -
    • Polyethylene glycol 400 0.66 v/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    188 μl of Ammonium sulfate (3.5 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    704 μl of water (100 v/v)
    A2
    • Ammonium sulfate 0.66 M -
    • Glycerol 3.3 v/v -
    • Magnesium sulfate 0 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    188 μl of Ammonium sulfate (3.5 M)
    33 μl of Glycerol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    0 μl of Magnesium sulfate (2 M)
    678 μl of water (100 v/v)
    A3
    • Ammonium sulfate 0.66 M -
    • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    188 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    708 μl of water (100 v/v)
    A4
    • Ammonium sulfate 0.66 M -
    • Polyethylene glycol 400 1.65 v/v -
    • Magnesium sulfate 0.05 M -
    		NA
    188 μl of Ammonium sulfate (3.5 M)
    16 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of Magnesium sulfate (2 M)
    769 μl of water (100 v/v)
    A5
    • Sodium chloride 1.29 M -
    • Polyethylene glycol 400 0.66 v/v -
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    50 μl of Magnesium chloride (2 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    257 μl of Sodium chloride (5 M)
    586 μl of water (100 v/v)
    A6
    • Sodium chloride 0.99 M -
    • 2-methyl-2,4-pentanediol BCC 1.65 v/v -
    • Calcium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    50 μl of Calcium chloride (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    16 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    198 μl of Sodium chloride (5 M)
    635 μl of water (100 v/v)
    A7
    • Sodium chloride 1.32 M -
    • 2-Propanol 1.65 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    16 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    264 μl of Sodium chloride (5 M)
    619 μl of water (100 v/v)
    A8
    • 2-Propanol 1.65 v/v -
    • Potassium Sodium phosphate 0.825 M pH 5.5
    		pH 5.5
    16 μl of 2-Propanol (100 v/v)
    275 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    708 μl of water (100 v/v)
    A9
    • Polyethylene glycol 400 0.66 v/v -
    • Potassium Sodium phosphate 0.66 M pH 6.5
    		pH 6.5
    220 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    773 μl of water (100 v/v)
    A10
    • Glycerol 6.6 v/v -
    • Potassium Sodium phosphate 0.825 M pH 7.5
    		pH 7.5
    66 μl of Glycerol (100 v/v)
    275 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    659 μl of water (100 v/v)
    A11
    • 2-methyl-2,4-pentanediol BCC 2.64 v/v -
    • Potassium Sodium phosphate 0.33 M pH 8.5
    		pH 8.5
    165 μl of Potassium Sodium phosphate pH 8.5 (2 M)
    26 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    808 μl of water (100 v/v)
    A12
    • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
    • tri-Ammonium citrate 0.66 M pH 4.5
    		pH 4.5
    3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    220 μl of tri-Ammonium citrate pH 4.5 (3 M)
    776 μl of water (100 v/v)
    B1
    • 2-Propanol 1.65 v/v -
    • tri-Ammonium citrate 0.66 M pH 6.5
    		pH 6.5
    16 μl of 2-Propanol (100 v/v)
    217 μl of tri-Ammonium citrate pH 7.5 (3 M)
    2 μl of tri-Ammonium citrate pH 4.5 (3 M)
    763 μl of water (100 v/v)
    B2
    • Polyethylene glycol 400 1.65 v/v -
    • tri-Ammonium citrate 0.66 M pH 7.5
    		pH 7.5
    220 μl of tri-Ammonium citrate pH 7.5 (3 M)
    16 μl of Polyethylene glycol 400 (100 v/v)
    763 μl of water (100 v/v)
    B3
    • Lithium sulfate 0.66 M -
    • 2-Propanol 1.65 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    16 μl of 2-Propanol (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    264 μl of Lithium sulfate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    569 μl of water (100 v/v)
    B4
    • Lithium sulfate 0.66 M -
    • Polyethylene glycol 400 1.65 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    16 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    264 μl of Lithium sulfate (2.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    569 μl of water (100 v/v)
    B5
    • Lithium sulfate 0.561 M -
    • 2-methyl-2,4-pentanediol BCC 2.24 v/v -
    • Imidazole 0.085 M pH 6.5
    		pH 6.5
    85 μl of Imidazole pH 6.5 (1 M)
    22 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    224 μl of Lithium sulfate (2.5 M)
    668 μl of water (100 v/v)
    B6
    • Lithium sulfate 0.66 M -
    • Polyethylene glycol 400 0.66 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    6 μl of Polyethylene glycol 400 (100 v/v)
    264 μl of Lithium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    629 μl of water (100 v/v)
    B7
    • Lithium sulfate 0.314 M -
    • 2-methyl-2,4-pentanediol BCC 4.7 v/v -
    • Magnesium sulfate 0.095 M -
    • Sodium acetate 0.095 M pH 4.5
    		pH 4.5
    47 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    47 μl of Magnesium sulfate (2 M)
    125 μl of Lithium sulfate (2.5 M)
    95 μl of Sodium acetate pH 4.5 (1 M)
    684 μl of water (100 v/v)
    B8
    • 2-methyl-2,4-pentanediol BCC 7.42 v/v -
    • tri-Ammonium citrate 0.223 M pH 5.5
    		pH 5.5
    0 μl of tri-Ammonium citrate pH 7.5 (3 M)
    74 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    74 μl of tri-Ammonium citrate pH 4.5 (3 M)
    851 μl of water (100 v/v)
    B9
    • Ammonium sulfate 0.495 M -
    • 2-Propanol 3.96 v/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    39 μl of 2-Propanol (100 v/v)
    141 μl of Ammonium sulfate (3.5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    719 μl of water (100 v/v)
    B10
    • Sodium chloride 0.429 M -
    • 2-Propanol 9.9 v/v -
    • Calcium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    99 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    85 μl of Sodium chloride (5 M)
    665 μl of water (100 v/v)
    B11
    • Sodium chloride 1.32 M -
    • Polyethylene glycol 400 3.3 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    264 μl of Sodium chloride (5 M)
    603 μl of water (100 v/v)
    B12
    • Polyethylene glycol 400 6.6 v/v -
    • Potassium Sodium phosphate 0.165 M pH 7.5
    		pH 7.5
    66 μl of Polyethylene glycol 400 (100 v/v)
    55 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    879 μl of water (100 v/v)
    C1
    • 2-Propanol 4.95 v/v -
    • tri-Ammonium citrate 0.33 M pH 8.5
    		pH 8.5
    49 μl of 2-Propanol (100 v/v)
    165 μl of tri-Ammonium citrate pH 8.5 (2 M)
    785 μl of water (100 v/v)
    C2
    • Sodium formate 0.66 M -
    • 2-Propanol 4.95 v/v -
    • Polyethylene glycol 3,350 0.825 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    49 μl of 2-Propanol (100 v/v)
    16 μl of Polyethylene glycol 3,350 (50 w/v)
    94 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    739 μl of water (100 v/v)
    C3
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 1,500 8.25 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    165 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    636 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 8,000 4.95 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    50 μl of Calcium chloride (2 M)
    99 μl of Polyethylene glycol 8,000 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    652 μl of water (100 v/v)
    C5
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 3,350 3.3 w/v -
    • Ammonium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    66 μl of Polyethylene glycol 3,350 (50 w/v)
    677 μl of water (100 v/v)
    C6
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 3,350 1.32 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    26 μl of Polyethylene glycol 3,350 (50 w/v)
    774 μl of water (100 v/v)
    C7
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 8,000 2.64 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    52 μl of Polyethylene glycol 8,000 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    648 μl of water (100 v/v)
    C8
    • 2-Propanol 9.9 v/v -
    • Polyethylene glycol 3,350 1.32 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    99 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    26 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    724 μl of water (100 v/v)
    C9
    • 2-Propanol 9.9 v/v -
    • Polyethylene glycol 1,500 3.3 w/v -
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    99 μl of 2-Propanol (100 v/v)
    66 μl of Polyethylene glycol 1,500 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    655 μl of water (100 v/v)
    C10
    • 2-Propanol 13.2 v/v -
    • Polyethylene glycol 8,000 4.95 w/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    132 μl of 2-Propanol (100 v/v)
    99 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    669 μl of water (100 v/v)
    C11
    • 2-Propanol 6.6 v/v -
    • Polyethylene glycol 3,350 4.95 w/v -
    • tri-Ammonium citrate 0.2 M pH 7.5
    		pH 7.5
    66 μl of 2-Propanol (100 v/v)
    66 μl of tri-Ammonium citrate pH 7.5 (3 M)
    99 μl of Polyethylene glycol 3,350 (50 w/v)
    768 μl of water (100 v/v)
    C12
    • 2-Propanol 9.9 v/v -
    • Polyethylene glycol 3,350 9.9 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    99 μl of 2-Propanol (100 v/v)
    198 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    603 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 13.2 v/v -
    • Polyethylene glycol 8,000 6.6 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    132 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    636 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 13.2 v/v -
    • Polyethylene glycol 3,350 1.65 w/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    132 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    735 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 6.6 v/v -
    • Polyethylene glycol 1,000 4.95 w/v -
    • Potassium Sodium phosphate 0.15 M pH 6.5
    		pH 6.5
    50 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    66 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    99 μl of Polyethylene glycol 1,000 (50 w/v)
    785 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 13.2 v/v -
    • Polyethylene glycol 8,000 2.64 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    52 μl of Polyethylene glycol 8,000 (50 w/v)
    132 μl of Polyethylene glycol 400 (100 v/v)
    715 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 8.25 v/v -
    • Polyethylene glycol 3,350 6.6 w/v -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    50 μl of Magnesium chloride (2 M)
    82 μl of Polyethylene glycol 400 (100 v/v)
    132 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    635 μl of water (100 v/v)
    D6
    • Polyethylene glycol 1,500 9.9 w/v -
    • 2-methyl-2,4-pentanediol BCC 0.99 v/v -
    • Magnesium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    198 μl of Polyethylene glycol 1,500 (50 w/v)
    9 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Magnesium sulfate (2 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    592 μl of water (100 v/v)
    D7
    • Polyethylene glycol 1,500 9.9 w/v -
    • 2-Propanol 3.3 v/v -
    • Calcium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    33 μl of 2-Propanol (100 v/v)
    198 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Calcium chloride (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    619 μl of water (100 v/v)
    D8
    • Polyethylene glycol 1,500 9.9 w/v -
    • Polyethylene glycol 400 6.6 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    198 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Polyethylene glycol 400 (100 v/v)
    636 μl of water (100 v/v)
    D9
    • Polyethylene glycol 1,500 9.9 w/v -
    • 2-methyl-2,4-pentanediol BCC 2.64 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    198 μl of Polyethylene glycol 1,500 (50 w/v)
    26 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    675 μl of water (100 v/v)
    D10
    • Polyethylene glycol 3,350 8.25 w/v -
    • 2-Propanol 4.95 v/v -
    • tri-Ammonium citrate 0.2 M pH 4.5
    		pH 4.5
    49 μl of 2-Propanol (100 v/v)
    165 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of tri-Ammonium citrate pH 4.5 (3 M)
    718 μl of water (100 v/v)
    D11
    • Polyethylene glycol 3,350 8.25 w/v -
    • Polyethylene glycol 400 1.65 v/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    16 μl of Polyethylene glycol 400 (100 v/v)
    165 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    718 μl of water (100 v/v)
    D12
    • Polyethylene glycol 3,350 8.25 w/v -
    • 2-methyl-2,4-pentanediol BCC 4.95 v/v -
    • Lithium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    100 μl of Imidazole pH 6.5 (1 M)
    49 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    165 μl of Polyethylene glycol 3,350 (50 w/v)
    605 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,350 8.25 w/v -
    • 2-Propanol 1.32 v/v -
    • Calcium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    13 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    165 μl of Polyethylene glycol 3,350 (50 w/v)
    671 μl of water (100 v/v)
    E2
    • Polyethylene glycol 8,000 6.6 w/v -
    • Polyethylene glycol 400 3.3 v/v -
    • Sodium chloride 0.5 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    33 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    100 μl of Sodium chloride (5 M)
    635 μl of water (100 v/v)
    E3
    • Polyethylene glycol 8,000 6.6 w/v -
    • 2-methyl-2,4-pentanediol BCC 0.99 v/v -
    • tri-Sodium citrate 0.1 M pH 6.5
    		pH 6.5
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 6.5 (1 M)
    9 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    758 μl of water (100 v/v)
    E4
    • Polyethylene glycol 8,000 6.6 w/v -
    • 2-Propanol 3.3 v/v -
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    33 μl of 2-Propanol (100 v/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    677 μl of water (100 v/v)
    E5
    • Polyethylene glycol 8,000 6.6 w/v -
    • Polyethylene glycol 400 6.6 v/v -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    50 μl of Magnesium chloride (2 M)
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    652 μl of water (100 v/v)
    E6
    • Sodium formate 0.693 M -
    • Polyethylene glycol 3,350 8.25 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    50 μl of Calcium chloride (2 M)
    165 μl of Polyethylene glycol 3,350 (50 w/v)
    99 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    586 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.247 M -
    • Polyethylene glycol 3,350 2.48 w/v -
    • 2-Propanol 1.65 v/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    16 μl of 2-Propanol (100 v/v)
    70 μl of Ammonium sulfate (3.5 M)
    49 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    763 μl of water (100 v/v)
    E8
    • Polyethylene glycol 400 0.33 v/v -
    • tri-Ammonium citrate 0.33 M pH 5.5
    		pH 5.5
    0 μl of tri-Ammonium citrate pH 7.5 (3 M)
    3 μl of Polyethylene glycol 400 (100 v/v)
    110 μl of tri-Ammonium citrate pH 4.5 (3 M)
    886 μl of water (100 v/v)
    E9
    • Sodium chloride 0.825 M -
    • Polyethylene glycol 1,500 3.96 w/v -
    • 2-methyl-2,4-pentanediol BCC 0.495 v/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    79 μl of Polyethylene glycol 1,500 (50 w/v)
    5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    165 μl of Sodium chloride (5 M)
    650 μl of water (100 v/v)
    E10
    • Sodium chloride 0.66 M -
    • Polyethylene glycol 3,350 6.6 w/v -
    • Magnesium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    50 μl of Magnesium chloride (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    132 μl of Polyethylene glycol 3,350 (50 w/v)
    132 μl of Sodium chloride (5 M)
    586 μl of water (100 v/v)
    E11
    • Sodium formate 0.99 M -
    • Polyethylene glycol 8,000 1.32 w/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    26 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    141 μl of Sodium formate (7 M)
    732 μl of water (100 v/v)
    E12
    • Polyethylene glycol 4,000 0.17 w/v -
    • Potassium Sodium phosphate 0.33 M pH 7.5
    		pH 7.5
    110 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    3 μl of Polyethylene glycol 4,000 (50 w/v)
    886 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 3.85 w/v -
    • Potassium Sodium phosphate 0.165 M pH 7.5
    		pH 7.5
    55 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    77 μl of Polyethylene glycol 3,350 (50 w/v)
    868 μl of water (100 v/v)
    F2
    • Polyethylene glycol 8,000 0.66 w/v -
    • tri-Ammonium citrate 0.165 M pH 8.5
    		pH 8.5
    13 μl of Polyethylene glycol 8,000 (50 w/v)
    82 μl of tri-Ammonium citrate pH 8.5 (2 M)
    904 μl of water (100 v/v)
    F3
    • Sodium chloride 0.66 M -
    • Polyethylene glycol 4,000 1.65 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    33 μl of Polyethylene glycol 4,000 (50 w/v)
    132 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    735 μl of water (100 v/v)
    F4
    • Polyethylene glycol 8,000 4.95 w/v -
    • tri-Ammonium citrate 0.165 M pH 8.5
    		pH 8.5
    99 μl of Polyethylene glycol 8,000 (50 w/v)
    82 μl of tri-Ammonium citrate pH 8.5 (2 M)
    818 μl of water (100 v/v)
    F5NA
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    F6NA
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    F7NA
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    F8NA
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    F9NA
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    F10NA
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    F11NA
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    F12NA
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    G1NA
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    G2NA
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    G3NA
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    G4NA
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    G5NA
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    G6NA
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    G7NA
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    G8NA
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    G9NA
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    G10NA
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    G11NA
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    G12NA
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    H1NA
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    H2NA
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    H3NA
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    H4NA
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    H5NA
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    H6NA
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    H7NA
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    H8NA
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    H9NA
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    H10NA
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    H11NA
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    H12NA
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