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Supplemental Information

Screen Description

Precipitant Synergy 33%

Rigaku / Precipitant Synergy 33%
Created 2009-11-16.

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Precipitant Synergy 33%

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Screen Structure

Precipitant Synergy 33%

Well Count 96
Number of distinct ingredients 23
Internal diversity (score mean dist) 0.894

Screen Composition

2025-08-16T09:05:39.082477 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Precipitant Synergy 33%
Buffer 66
Cryoprotectant 2
Organic (non-volatile) 38
Organic (volatile) 18
Polymer 57
Salt 73

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Precipitant Synergy 33% Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 0.66 M -
  • Polyethylene glycol 400 0.66 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
188.6 μl of Ammonium sulfate (3.5 M)
6.6 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
704.8 μl of water (100 v/v)
A2
  • Ammonium sulfate 0.66 M -
  • Glycerol 3.3 v/v -
  • Magnesium sulfate 0 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
188.6 μl of Ammonium sulfate (3.5 M)
33.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
0.0 μl of Magnesium sulfate (2 M)
678.4 μl of water (100 v/v)
A3
  • Ammonium sulfate 0.66 M -
  • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
3.3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
188.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
708.1 μl of water (100 v/v)
A4
  • Ammonium sulfate 0.66 M -
  • Polyethylene glycol 400 1.65 v/v -
  • Magnesium sulfate 0.05 M -
NA
188.6 μl of Ammonium sulfate (3.5 M)
25.0 μl of Magnesium sulfate (2 M)
16.5 μl of Polyethylene glycol 400 (100 v/v)
769.9 μl of water (100 v/v)
A5
  • Sodium chloride 1.29 M -
  • Polyethylene glycol 400 0.66 v/v -
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
50.0 μl of Magnesium chloride (2 M)
6.6 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
257.4 μl of Sodium chloride (5 M)
586.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.99 M -
  • 2-methyl-2,4-pentanediol BCC 1.65 v/v -
  • Calcium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
16.5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of Imidazole pH 6.5 (1 M)
198.0 μl of Sodium chloride (5 M)
635.5 μl of water (100 v/v)
A7
  • Sodium chloride 1.32 M -
  • 2-Propanol 1.65 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
16.5 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
264.0 μl of Sodium chloride (5 M)
619.5 μl of water (100 v/v)
A8
  • 2-Propanol 1.65 v/v -
  • Potassium Sodium phosphate 0.825 M pH 5.5
pH 5.5
16.5 μl of 2-Propanol (100 v/v)
275.0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
708.5 μl of water (100 v/v)
A9
  • Polyethylene glycol 400 0.66 v/v -
  • Potassium Sodium phosphate 0.66 M pH 6.5
pH 6.5
6.6 μl of Polyethylene glycol 400 (100 v/v)
200.0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
20.0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
773.4 μl of water (100 v/v)
A10
  • Glycerol 6.6 v/v -
  • Potassium Sodium phosphate 0.825 M pH 7.5
pH 7.5
66.0 μl of Glycerol (100 v/v)
275.0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
659.0 μl of water (100 v/v)
A11
  • 2-methyl-2,4-pentanediol BCC 2.64 v/v -
  • Potassium Sodium phosphate 0.33 M pH 8.5
pH 8.5
26.4 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
165.0 μl of Potassium Sodium phosphate pH 8.5 (2 M)
808.6 μl of water (100 v/v)
A12
  • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
  • tri-Ammonium citrate 0.66 M pH 4.5
pH 4.5
3.3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
220.0 μl of tri-Ammonium citrate pH 4.5 (3 M)
776.7 μl of water (100 v/v)
B1
  • 2-Propanol 1.65 v/v -
  • tri-Ammonium citrate 0.66 M pH 6.5
pH 6.5
16.5 μl of 2-Propanol (100 v/v)
20.0 μl of tri-Ammonium citrate pH 4.5 (3 M)
200.0 μl of tri-Ammonium citrate pH 7.5 (3 M)
763.5 μl of water (100 v/v)
B2
  • Polyethylene glycol 400 1.65 v/v -
  • tri-Ammonium citrate 0.66 M pH 7.5
pH 7.5
16.5 μl of Polyethylene glycol 400 (100 v/v)
220.0 μl of tri-Ammonium citrate pH 7.5 (3 M)
763.5 μl of water (100 v/v)
B3
  • Lithium sulfate 0.66 M -
  • 2-Propanol 1.65 v/v -
  • Magnesium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
16.5 μl of 2-Propanol (100 v/v)
264.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
569.5 μl of water (100 v/v)
B4
  • Lithium sulfate 0.66 M -
  • Polyethylene glycol 400 1.65 v/v -
  • Magnesium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
264.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium sulfate (2 M)
16.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
569.5 μl of water (100 v/v)
B5
  • Lithium sulfate 0.561 M -
  • 2-methyl-2,4-pentanediol BCC 2.24 v/v -
  • Imidazole 0.085 M pH 6.5
pH 6.5
22.4 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
85.0 μl of Imidazole pH 6.5 (1 M)
224.4 μl of Lithium sulfate (2.5 M)
668.2 μl of water (100 v/v)
B6
  • Lithium sulfate 0.66 M -
  • Polyethylene glycol 400 0.66 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
264.0 μl of Lithium sulfate (2.5 M)
6.6 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
629.4 μl of water (100 v/v)
B7
  • Lithium sulfate 0.314 M -
  • 2-methyl-2,4-pentanediol BCC 4.7 v/v -
  • Magnesium sulfate 0.095 M -
  • Sodium acetate 0.095 M pH 4.5
pH 4.5
47.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
125.6 μl of Lithium sulfate (2.5 M)
47.5 μl of Magnesium sulfate (2 M)
95.0 μl of Sodium acetate pH 4.5 (1 M)
684.9 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 7.42 v/v -
  • tri-Ammonium citrate 0.223 M pH 5.5
pH 5.5
74.2 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
67.6 μl of tri-Ammonium citrate pH 4.5 (3 M)
6.8 μl of tri-Ammonium citrate pH 7.5 (3 M)
851.4 μl of water (100 v/v)
B9
  • Ammonium sulfate 0.495 M -
  • 2-Propanol 3.96 v/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
39.6 μl of 2-Propanol (100 v/v)
141.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Imidazole pH 6.5 (1 M)
719.0 μl of water (100 v/v)
B10
  • Sodium chloride 0.429 M -
  • 2-Propanol 9.9 v/v -
  • Calcium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
99.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of Imidazole pH 6.5 (1 M)
85.8 μl of Sodium chloride (5 M)
665.2 μl of water (100 v/v)
B11
  • Sodium chloride 1.32 M -
  • Polyethylene glycol 400 3.3 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
33.0 μl of Polyethylene glycol 400 (100 v/v)
264.0 μl of Sodium chloride (5 M)
603.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 400 6.6 v/v -
  • Potassium Sodium phosphate 0.165 M pH 7.5
pH 7.5
66.0 μl of Polyethylene glycol 400 (100 v/v)
55.0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
879.0 μl of water (100 v/v)
C1
  • 2-Propanol 4.95 v/v -
  • tri-Ammonium citrate 0.33 M pH 8.5
pH 8.5
49.5 μl of 2-Propanol (100 v/v)
165.0 μl of tri-Ammonium citrate pH 8.5 (2 M)
785.5 μl of water (100 v/v)
C2
  • Sodium formate 0.66 M -
  • 2-Propanol 4.95 v/v -
  • Polyethylene glycol 3,350 0.825 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
49.5 μl of 2-Propanol (100 v/v)
16.5 μl of Polyethylene glycol 3,350 (50 w/v)
94.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
739.7 μl of water (100 v/v)
C3
  • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
  • Polyethylene glycol 1,500 8.25 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
99.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
165.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
636.0 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
  • Polyethylene glycol 8,000 4.95 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
99.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Calcium chloride (2 M)
99.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
652.0 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
  • Polyethylene glycol 3,350 3.3 w/v -
  • Ammonium sulfate 0.2 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
99.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Imidazole pH 6.5 (1 M)
66.0 μl of Polyethylene glycol 3,350 (50 w/v)
677.9 μl of water (100 v/v)
C6
  • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
  • Polyethylene glycol 3,350 1.32 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
99.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
26.4 μl of Polyethylene glycol 3,350 (50 w/v)
774.6 μl of water (100 v/v)
C7
  • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
  • Polyethylene glycol 8,000 2.64 w/v -
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
99.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
52.8 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
648.2 μl of water (100 v/v)
C8
  • 2-Propanol 9.9 v/v -
  • Polyethylene glycol 3,350 1.32 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
99.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
26.4 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
724.6 μl of water (100 v/v)
C9
  • 2-Propanol 9.9 v/v -
  • Polyethylene glycol 1,500 3.3 w/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
99.0 μl of 2-Propanol (100 v/v)
80.0 μl of Lithium sulfate (2.5 M)
66.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
655.0 μl of water (100 v/v)
C10
  • 2-Propanol 13.2 v/v -
  • Polyethylene glycol 8,000 4.95 w/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
132.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
99.0 μl of Polyethylene glycol 8,000 (50 w/v)
669.0 μl of water (100 v/v)
C11
  • 2-Propanol 6.6 v/v -
  • Polyethylene glycol 3,350 4.95 w/v -
  • tri-Ammonium citrate 0.2 M pH 7.5
pH 7.5
66.0 μl of 2-Propanol (100 v/v)
99.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 7.5 (3 M)
768.3 μl of water (100 v/v)
C12
  • 2-Propanol 9.9 v/v -
  • Polyethylene glycol 3,350 9.9 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
99.0 μl of 2-Propanol (100 v/v)
198.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
603.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 400 13.2 v/v -
  • Polyethylene glycol 8,000 6.6 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
132.0 μl of Polyethylene glycol 400 (100 v/v)
132.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
636.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 13.2 v/v -
  • Polyethylene glycol 3,350 1.65 w/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
33.0 μl of Polyethylene glycol 3,350 (50 w/v)
132.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
735.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 400 6.6 v/v -
  • Polyethylene glycol 1,000 4.95 w/v -
  • Potassium Sodium phosphate 0.15 M pH 6.5
pH 6.5
99.0 μl of Polyethylene glycol 1,000 (50 w/v)
66.0 μl of Polyethylene glycol 400 (100 v/v)
45.5 μl of Potassium Sodium phosphate pH 5.5 (3 M)
4.5 μl of Potassium Sodium phosphate pH 7.5 (3 M)
785.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 400 13.2 v/v -
  • Polyethylene glycol 8,000 2.64 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
132.0 μl of Polyethylene glycol 400 (100 v/v)
52.8 μl of Polyethylene glycol 8,000 (50 w/v)
715.2 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 8.25 v/v -
  • Polyethylene glycol 3,350 6.6 w/v -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of Magnesium chloride (2 M)
132.0 μl of Polyethylene glycol 3,350 (50 w/v)
82.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
635.5 μl of water (100 v/v)
D6
  • Polyethylene glycol 1,500 9.9 w/v -
  • 2-methyl-2,4-pentanediol BCC 0.99 v/v -
  • Magnesium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
9.9 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Magnesium sulfate (2 M)
198.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
592.1 μl of water (100 v/v)
D7
  • Polyethylene glycol 1,500 9.9 w/v -
  • 2-Propanol 3.3 v/v -
  • Calcium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
33.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of Imidazole pH 6.5 (1 M)
198.0 μl of Polyethylene glycol 1,500 (50 w/v)
619.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 1,500 9.9 w/v -
  • Polyethylene glycol 400 6.6 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
198.0 μl of Polyethylene glycol 1,500 (50 w/v)
66.0 μl of Polyethylene glycol 400 (100 v/v)
636.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 1,500 9.9 w/v -
  • 2-methyl-2,4-pentanediol BCC 2.64 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
26.4 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
198.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
675.6 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 8.25 w/v -
  • 2-Propanol 4.95 v/v -
  • tri-Ammonium citrate 0.2 M pH 4.5
pH 4.5
49.5 μl of 2-Propanol (100 v/v)
165.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 4.5 (3 M)
718.8 μl of water (100 v/v)
D11
  • Polyethylene glycol 3,350 8.25 w/v -
  • Polyethylene glycol 400 1.65 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
165.0 μl of Polyethylene glycol 3,350 (50 w/v)
16.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
718.5 μl of water (100 v/v)
D12
  • Polyethylene glycol 3,350 8.25 w/v -
  • 2-methyl-2,4-pentanediol BCC 4.95 v/v -
  • Lithium sulfate 0.2 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
49.5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
80.0 μl of Lithium sulfate (2.5 M)
165.0 μl of Polyethylene glycol 3,350 (50 w/v)
605.5 μl of water (100 v/v)
E1
  • Polyethylene glycol 3,350 8.25 w/v -
  • 2-Propanol 1.32 v/v -
  • Calcium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
13.2 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
165.0 μl of Polyethylene glycol 3,350 (50 w/v)
671.8 μl of water (100 v/v)
E2
  • Polyethylene glycol 8,000 6.6 w/v -
  • Polyethylene glycol 400 3.3 v/v -
  • Sodium chloride 0.5 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
33.0 μl of Polyethylene glycol 400 (100 v/v)
132.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium chloride (5 M)
635.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 8,000 6.6 w/v -
  • 2-methyl-2,4-pentanediol BCC 0.99 v/v -
  • tri-Sodium citrate 0.1 M pH 6.5
pH 6.5
9.9 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
132.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 6.5 (1 M)
758.1 μl of water (100 v/v)
E4
  • Polyethylene glycol 8,000 6.6 w/v -
  • 2-Propanol 3.3 v/v -
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
33.0 μl of 2-Propanol (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
132.0 μl of Polyethylene glycol 8,000 (50 w/v)
677.9 μl of water (100 v/v)
E5
  • Polyethylene glycol 8,000 6.6 w/v -
  • Polyethylene glycol 400 6.6 v/v -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of Magnesium chloride (2 M)
66.0 μl of Polyethylene glycol 400 (100 v/v)
132.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
652.0 μl of water (100 v/v)
E6
  • Sodium formate 0.693 M -
  • Polyethylene glycol 3,350 8.25 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
50.0 μl of Calcium chloride (2 M)
165.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
99.0 μl of Sodium formate (7 M)
586.0 μl of water (100 v/v)
E7
  • Ammonium sulfate 0.247 M -
  • Polyethylene glycol 3,350 2.48 w/v -
  • 2-Propanol 1.65 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
16.5 μl of 2-Propanol (100 v/v)
70.7 μl of Ammonium sulfate (3.5 M)
49.6 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
763.2 μl of water (100 v/v)
E8
  • Polyethylene glycol 400 0.33 v/v -
  • tri-Ammonium citrate 0.33 M pH 5.5
pH 5.5
3.3 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Ammonium citrate pH 4.5 (3 M)
10.0 μl of tri-Ammonium citrate pH 7.5 (3 M)
886.7 μl of water (100 v/v)
E9
  • Sodium chloride 0.825 M -
  • Polyethylene glycol 1,500 3.96 w/v -
  • 2-methyl-2,4-pentanediol BCC 0.495 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
5.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
79.2 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
165.0 μl of Sodium chloride (5 M)
650.9 μl of water (100 v/v)
E10
  • Sodium chloride 0.66 M -
  • Polyethylene glycol 3,350 6.6 w/v -
  • Magnesium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
132.0 μl of Polyethylene glycol 3,350 (50 w/v)
132.0 μl of Sodium chloride (5 M)
586.0 μl of water (100 v/v)
E11
  • Sodium formate 0.99 M -
  • Polyethylene glycol 8,000 1.32 w/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
26.4 μl of Polyethylene glycol 8,000 (50 w/v)
141.4 μl of Sodium formate (7 M)
732.2 μl of water (100 v/v)
E12
  • Polyethylene glycol 4,000 0.17 w/v -
  • Potassium Sodium phosphate 0.33 M pH 7.5
pH 7.5
3.4 μl of Polyethylene glycol 4,000 (50 w/v)
110.0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
886.6 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 3.85 w/v -
  • Potassium Sodium phosphate 0.165 M pH 7.5
pH 7.5
77.0 μl of Polyethylene glycol 3,350 (50 w/v)
55.0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
868.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 8,000 0.66 w/v -
  • tri-Ammonium citrate 0.165 M pH 8.5
pH 8.5
13.2 μl of Polyethylene glycol 8,000 (50 w/v)
82.5 μl of tri-Ammonium citrate pH 8.5 (2 M)
904.3 μl of water (100 v/v)
F3
  • Sodium chloride 0.66 M -
  • Polyethylene glycol 4,000 1.65 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
33.0 μl of Polyethylene glycol 4,000 (50 w/v)
132.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
735.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 8,000 4.95 w/v -
  • tri-Ammonium citrate 0.165 M pH 8.5
pH 8.5
99.0 μl of Polyethylene glycol 8,000 (50 w/v)
82.5 μl of tri-Ammonium citrate pH 8.5 (2 M)
818.5 μl of water (100 v/v)
F5NA
1000.0 μl of water (100 v/v)
F6NA
1000.0 μl of water (100 v/v)
F7NA
1000.0 μl of water (100 v/v)
F8NA
1000.0 μl of water (100 v/v)
F9NA
1000.0 μl of water (100 v/v)
F10NA
1000.0 μl of water (100 v/v)
F11NA
1000.0 μl of water (100 v/v)
F12NA
1000.0 μl of water (100 v/v)
G1NA
1000.0 μl of water (100 v/v)
G2NA
1000.0 μl of water (100 v/v)
G3NA
1000.0 μl of water (100 v/v)
G4NA
1000.0 μl of water (100 v/v)
G5NA
1000.0 μl of water (100 v/v)
G6NA
1000.0 μl of water (100 v/v)
G7NA
1000.0 μl of water (100 v/v)
G8NA
1000.0 μl of water (100 v/v)
G9NA
1000.0 μl of water (100 v/v)
G10NA
1000.0 μl of water (100 v/v)
G11NA
1000.0 μl of water (100 v/v)
G12NA
1000.0 μl of water (100 v/v)
H1NA
1000.0 μl of water (100 v/v)
H2NA
1000.0 μl of water (100 v/v)
H3NA
1000.0 μl of water (100 v/v)
H4NA
1000.0 μl of water (100 v/v)
H5NA
1000.0 μl of water (100 v/v)
H6NA
1000.0 μl of water (100 v/v)
H7NA
1000.0 μl of water (100 v/v)
H8NA
1000.0 μl of water (100 v/v)
H9NA
1000.0 μl of water (100 v/v)
H10NA
1000.0 μl of water (100 v/v)
H11NA
1000.0 μl of water (100 v/v)
H12NA
1000.0 μl of water (100 v/v)