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Supplemental Information

Screen Description

Precipitant Synergy 67%

Rigaku / Precipitant Synergy 67%
Created 2009-11-16.

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Precipitant Synergy 67%

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Screen Structure

Precipitant Synergy 67%

Well Count 96
Number of distinct ingredients 24
Internal diversity (score mean dist) 0.904

Screen Composition

2025-08-16T09:05:39.370897 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Precipitant Synergy 67%
Buffer 66
Cryoprotectant 2
Organic (non-volatile) 37
Organic (volatile) 18
Polymer 56
Salt 74

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Precipitant Synergy 67% Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 1.34 M -
  • Polyethylene glycol 400 1.34 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
382.9 μl of Ammonium sulfate (3.5 M)
13.4 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
503.7 μl of water (100 v/v)
A2
  • Ammonium sulfate 1.34 M -
  • Glycerol 6.7 v/v -
  • Magnesium chloride 0.05 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
382.9 μl of Ammonium sulfate (3.5 M)
67.0 μl of Glycerol (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
25.0 μl of Magnesium chloride (2 M)
425.1 μl of water (100 v/v)
A3
  • Ammonium sulfate 1.34 M -
  • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
6.7 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
382.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
510.4 μl of water (100 v/v)
A4
  • Ammonium sulfate 1.34 M -
  • Polyethylene glycol 400 3.35 v/v -
  • Magnesium sulfate 0.05 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
382.9 μl of Ammonium sulfate (3.5 M)
25.0 μl of Magnesium sulfate (2 M)
33.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
458.6 μl of water (100 v/v)
A5
  • Sodium chloride 2.61 M -
  • Polyethylene glycol 400 1.34 v/v -
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
50.0 μl of Magnesium chloride (2 M)
13.4 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
522.6 μl of Sodium chloride (5 M)
314.0 μl of water (100 v/v)
A6
  • Sodium chloride 2.01 M -
  • 2-methyl-2,4-pentanediol BCC 3.35 v/v -
  • Calcium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
33.5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of Imidazole pH 6.5 (1 M)
402.0 μl of Sodium chloride (5 M)
414.5 μl of water (100 v/v)
A7
  • Sodium chloride 2.68 M -
  • 2-Propanol 3.35 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
33.5 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
536.0 μl of Sodium chloride (5 M)
330.5 μl of water (100 v/v)
A8
  • 2-Propanol 3.35 v/v -
  • Potassium Sodium phosphate 1.68 M pH 5.5
pH 5.5
33.5 μl of 2-Propanol (100 v/v)
558.3 μl of Potassium Sodium phosphate pH 5.5 (3 M)
408.2 μl of water (100 v/v)
A9
  • Polyethylene glycol 400 1.34 v/v -
  • Potassium Sodium phosphate 1.34 M pH 6.5
pH 6.5
13.4 μl of Polyethylene glycol 400 (100 v/v)
406.1 μl of Potassium Sodium phosphate pH 5.5 (3 M)
40.6 μl of Potassium Sodium phosphate pH 7.5 (3 M)
539.9 μl of water (100 v/v)
A10
  • Glycerol 13.4 v/v -
  • Potassium Sodium phosphate 1.68 M pH 7.5
pH 7.5
134.0 μl of Glycerol (100 v/v)
558.3 μl of Potassium Sodium phosphate pH 7.5 (3 M)
307.7 μl of water (100 v/v)
A11
  • 2-methyl-2,4-pentanediol BCC 5.36 v/v -
  • Potassium Sodium phosphate 0.67 M pH 8.5
pH 8.5
53.6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
335.0 μl of Potassium Sodium phosphate pH 8.5 (2 M)
611.4 μl of water (100 v/v)
A12
  • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
  • tri-Ammonium citrate 1.34 M pH 4.5
pH 4.5
6.7 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
446.7 μl of tri-Ammonium citrate pH 4.5 (3 M)
546.6 μl of water (100 v/v)
B1
  • 2-Propanol 3.35 v/v -
  • tri-Ammonium citrate 1.34 M pH 6.5
pH 6.5
33.5 μl of 2-Propanol (100 v/v)
40.6 μl of tri-Ammonium citrate pH 4.5 (3 M)
406.1 μl of tri-Ammonium citrate pH 7.5 (3 M)
519.8 μl of water (100 v/v)
B2
  • Polyethylene glycol 400 3.35 v/v -
  • tri-Ammonium citrate 1.34 M pH 7.5
pH 7.5
33.5 μl of Polyethylene glycol 400 (100 v/v)
446.7 μl of tri-Ammonium citrate pH 7.5 (3 M)
519.8 μl of water (100 v/v)
B3
  • Lithium sulfate 1.34 M -
  • 2-Propanol 3.35 v/v -
  • Magnesium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
33.5 μl of 2-Propanol (100 v/v)
536.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
280.5 μl of water (100 v/v)
B4
  • Lithium sulfate 1.34 M -
  • Polyethylene glycol 400 3.35 v/v -
  • Magnesium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
536.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium sulfate (2 M)
33.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
280.5 μl of water (100 v/v)
B5
  • Lithium sulfate 1.14 M -
  • 2-methyl-2,4-pentanediol BCC 4.56 v/v -
  • Imidazole 0.085 M pH 6.5
pH 6.5
45.6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
85.0 μl of Imidazole pH 6.5 (1 M)
455.6 μl of Lithium sulfate (2.5 M)
413.8 μl of water (100 v/v)
B6
  • Lithium sulfate 1.34 M -
  • Polyethylene glycol 400 1.34 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
536.0 μl of Lithium sulfate (2.5 M)
13.4 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.6 μl of water (100 v/v)
B7
  • Lithium sulfate 0.636 M -
  • 2-methyl-2,4-pentanediol BCC 9.55 v/v -
  • Magnesium sulfate 0.095 M -
  • Cadmium chloride 0 M -
NA
95.5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
0.0 μl of Cadmium chloride (1 M)
254.6 μl of Lithium sulfate (2.5 M)
47.5 μl of Magnesium sulfate (2 M)
602.4 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 15.1 v/v -
  • tri-Ammonium citrate 0.453 M pH 5.5
pH 5.5
150.8 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
137.3 μl of tri-Ammonium citrate pH 4.5 (3 M)
13.7 μl of tri-Ammonium citrate pH 7.5 (3 M)
698.2 μl of water (100 v/v)
B9
  • Ammonium sulfate 1 M -
  • 2-Propanol 8.04 v/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
80.4 μl of 2-Propanol (100 v/v)
287.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Imidazole pH 6.5 (1 M)
532.5 μl of water (100 v/v)
B10
  • Sodium chloride 0.871 M -
  • 2-Propanol 20.1 v/v -
  • Calcium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 6.5
pH 6.5
201.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
174.2 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 6.5 (1 M)
474.8 μl of water (100 v/v)
B11
  • Sodium chloride 2.68 M -
  • Polyethylene glycol 400 6.7 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
67.0 μl of Polyethylene glycol 400 (100 v/v)
536.0 μl of Sodium chloride (5 M)
297.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 400 13.4 v/v -
  • Potassium Sodium phosphate 0.335 M pH 7.5
pH 7.5
134.0 μl of Polyethylene glycol 400 (100 v/v)
111.7 μl of Potassium Sodium phosphate pH 7.5 (3 M)
754.3 μl of water (100 v/v)
C1
  • 2-Propanol 10.1 v/v -
  • tri-Ammonium citrate 0.67 M pH 8.5
pH 8.5
100.5 μl of 2-Propanol (100 v/v)
335.0 μl of tri-Ammonium citrate pH 8.5 (2 M)
564.5 μl of water (100 v/v)
C2
  • Sodium formate 1.34 M -
  • 2-Propanol 10.1 v/v -
  • Polyethylene glycol 3,350 1.68 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.5 μl of 2-Propanol (100 v/v)
33.5 μl of Polyethylene glycol 3,350 (50 w/v)
191.4 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
574.6 μl of water (100 v/v)
C3
  • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
  • Polyethylene glycol 1,500 16.8 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
201.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
335.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
364.0 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
  • Polyethylene glycol 8,000 10.1 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
201.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Calcium chloride (2 M)
201.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
448.0 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
  • Polyethylene glycol 3,350 6.7 w/v -
  • Ammonium sulfate 0.2 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
201.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Imidazole pH 6.5 (1 M)
134.0 μl of Polyethylene glycol 3,350 (50 w/v)
507.9 μl of water (100 v/v)
C6
  • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
  • Cadmium chloride 0 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
201.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
0.0 μl of Cadmium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
699.0 μl of water (100 v/v)
C7
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • Polyethylene glycol 8,000 5.36 w/v -
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
107.2 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
492.8 μl of water (100 v/v)
C8
  • 2-Propanol 20.1 v/v -
  • Polyethylene glycol 3,350 2.68 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
201.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
53.6 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
595.4 μl of water (100 v/v)
C9
  • 2-Propanol 20.1 v/v -
  • Polyethylene glycol 1,500 6.7 w/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
201.0 μl of 2-Propanol (100 v/v)
80.0 μl of Lithium sulfate (2.5 M)
134.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
485.0 μl of water (100 v/v)
C10
  • 2-Propanol 26.8 v/v -
  • Polyethylene glycol 8,000 10.1 w/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
268.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
201.0 μl of Polyethylene glycol 8,000 (50 w/v)
431.0 μl of water (100 v/v)
C11
  • 2-Propanol 13.4 v/v -
  • Polyethylene glycol 3,350 10.1 w/v -
  • tri-Ammonium citrate 0.2 M pH 7.5
pH 7.5
134.0 μl of 2-Propanol (100 v/v)
201.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 7.5 (3 M)
598.3 μl of water (100 v/v)
C12
  • 2-Propanol 20.1 v/v -
  • Polyethylene glycol 3,350 20.1 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
201.0 μl of 2-Propanol (100 v/v)
402.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
297.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 400 26.8 v/v -
  • Polyethylene glycol 8,000 13.4 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
268.0 μl of Polyethylene glycol 400 (100 v/v)
268.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
364.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 26.8 v/v -
  • Polyethylene glycol 3,350 3.35 w/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
67.0 μl of Polyethylene glycol 3,350 (50 w/v)
268.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
565.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 400 13.4 v/v -
  • Polyethylene glycol 1,000 10.1 w/v -
  • Potassium Sodium phosphate 0.15 M pH 6.5
pH 6.5
201.0 μl of Polyethylene glycol 1,000 (50 w/v)
134.0 μl of Polyethylene glycol 400 (100 v/v)
45.5 μl of Potassium Sodium phosphate pH 5.5 (3 M)
4.5 μl of Potassium Sodium phosphate pH 7.5 (3 M)
615.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 400 26.8 v/v -
  • Polyethylene glycol 8,000 5.36 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
268.0 μl of Polyethylene glycol 400 (100 v/v)
107.2 μl of Polyethylene glycol 8,000 (50 w/v)
524.8 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 16.8 v/v -
  • Polyethylene glycol 3,350 13.4 w/v -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of Magnesium chloride (2 M)
268.0 μl of Polyethylene glycol 3,350 (50 w/v)
167.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
414.5 μl of water (100 v/v)
D6
  • Polyethylene glycol 1,500 20.1 w/v -
  • 2-methyl-2,4-pentanediol BCC 2.01 v/v -
  • Magnesium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
20.1 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Magnesium sulfate (2 M)
402.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
377.9 μl of water (100 v/v)
D7
  • Polyethylene glycol 1,500 20.1 w/v -
  • 2-Propanol 6.7 v/v -
  • Calcium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
67.0 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of Imidazole pH 6.5 (1 M)
402.0 μl of Polyethylene glycol 1,500 (50 w/v)
381.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 1,500 20.1 w/v -
  • Polyethylene glycol 400 13.4 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
402.0 μl of Polyethylene glycol 1,500 (50 w/v)
134.0 μl of Polyethylene glycol 400 (100 v/v)
364.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 1,500 20.1 w/v -
  • 2-methyl-2,4-pentanediol BCC 5.36 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
53.6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
402.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
444.4 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 16.8 w/v -
  • 2-Propanol 10.1 v/v -
  • tri-Ammonium citrate 0.2 M pH 4.5
pH 4.5
100.5 μl of 2-Propanol (100 v/v)
335.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 4.5 (3 M)
497.8 μl of water (100 v/v)
D11
  • Polyethylene glycol 3,350 16.8 w/v -
  • Polyethylene glycol 400 3.35 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
335.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.5 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
531.5 μl of water (100 v/v)
D12
  • Polyethylene glycol 3,350 16.8 w/v -
  • 2-methyl-2,4-pentanediol BCC 10.1 v/v -
  • Lithium sulfate 0.2 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.5 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
80.0 μl of Lithium sulfate (2.5 M)
335.0 μl of Polyethylene glycol 3,350 (50 w/v)
384.5 μl of water (100 v/v)
E1
  • Polyethylene glycol 3,350 16.8 w/v -
  • 2-Propanol 2.68 v/v -
  • Calcium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
26.8 μl of 2-Propanol (100 v/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
335.0 μl of Polyethylene glycol 3,350 (50 w/v)
488.2 μl of water (100 v/v)
E2
  • Polyethylene glycol 8,000 13.4 w/v -
  • Polyethylene glycol 400 6.7 v/v -
  • Sodium chloride 0.5 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
67.0 μl of Polyethylene glycol 400 (100 v/v)
268.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium chloride (5 M)
465.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 8,000 13.4 w/v -
  • 2-methyl-2,4-pentanediol BCC 2.01 v/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
20.1 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
268.0 μl of Polyethylene glycol 8,000 (50 w/v)
611.9 μl of water (100 v/v)
E4
  • Polyethylene glycol 8,000 13.4 w/v -
  • 2-Propanol 6.7 v/v -
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
67.0 μl of 2-Propanol (100 v/v)
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
268.0 μl of Polyethylene glycol 8,000 (50 w/v)
507.9 μl of water (100 v/v)
E5
  • Polyethylene glycol 8,000 13.4 w/v -
  • Polyethylene glycol 400 13.4 v/v -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
50.0 μl of Magnesium chloride (2 M)
134.0 μl of Polyethylene glycol 400 (100 v/v)
268.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
448.0 μl of water (100 v/v)
E6
  • Sodium formate 1.41 M -
  • Polyethylene glycol 3,350 16.8 w/v -
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
50.0 μl of Calcium chloride (2 M)
335.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
201.0 μl of Sodium formate (7 M)
314.0 μl of water (100 v/v)
E7
  • Ammonium sulfate 0.502 M -
  • Polyethylene glycol 3,350 5.03 w/v -
  • 2-Propanol 3.35 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
33.5 μl of 2-Propanol (100 v/v)
143.6 μl of Ammonium sulfate (3.5 M)
100.5 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
622.4 μl of water (100 v/v)
E8
  • Polyethylene glycol 4,000 0.67 w/v -
  • tri-Ammonium citrate 0.67 M pH 5.5
pH 5.5
13.4 μl of Polyethylene glycol 4,000 (50 w/v)
203.0 μl of tri-Ammonium citrate pH 4.5 (3 M)
20.3 μl of tri-Ammonium citrate pH 7.5 (3 M)
763.3 μl of water (100 v/v)
E9
  • Sodium chloride 1.68 M -
  • Polyethylene glycol 1,500 8.04 w/v -
  • 2-methyl-2,4-pentanediol BCC 1 v/v -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
10.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
160.8 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
335.0 μl of Sodium chloride (5 M)
394.1 μl of water (100 v/v)
E10
  • Sodium chloride 1.34 M -
  • Polyethylene glycol 3,350 13.4 w/v -
  • Magnesium chloride 0.1 M -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
268.0 μl of Polyethylene glycol 3,350 (50 w/v)
268.0 μl of Sodium chloride (5 M)
314.0 μl of water (100 v/v)
E11
  • Sodium formate 2.01 M -
  • Polyethylene glycol 8,000 2.68 w/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
53.6 μl of Polyethylene glycol 8,000 (50 w/v)
287.1 μl of Sodium formate (7 M)
559.3 μl of water (100 v/v)
E12
  • Polyethylene glycol 4,000 0.34 w/v -
  • Potassium Sodium phosphate 0.67 M pH 7.5
pH 7.5
6.8 μl of Polyethylene glycol 4,000 (50 w/v)
223.3 μl of Potassium Sodium phosphate pH 7.5 (3 M)
769.9 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 7.82 w/v -
  • Potassium Sodium phosphate 0.335 M pH 7.5
pH 7.5
156.4 μl of Polyethylene glycol 3,350 (50 w/v)
111.7 μl of Potassium Sodium phosphate pH 7.5 (3 M)
731.9 μl of water (100 v/v)
F2
  • Polyethylene glycol 8,000 1.34 w/v -
  • tri-Ammonium citrate 0.335 M pH 8.5
pH 8.5
26.8 μl of Polyethylene glycol 8,000 (50 w/v)
167.5 μl of tri-Ammonium citrate pH 8.5 (2 M)
805.7 μl of water (100 v/v)
F3
  • Sodium chloride 1.34 M -
  • Polyethylene glycol 4,000 3.35 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
67.0 μl of Polyethylene glycol 4,000 (50 w/v)
268.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
565.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 8,000 10.1 w/v -
  • tri-Ammonium citrate 0.335 M pH 8.5
pH 8.5
201.0 μl of Polyethylene glycol 8,000 (50 w/v)
167.5 μl of tri-Ammonium citrate pH 8.5 (2 M)
631.5 μl of water (100 v/v)
F5NA
1000.0 μl of water (100 v/v)
F6NA
1000.0 μl of water (100 v/v)
F7NA
1000.0 μl of water (100 v/v)
F8NA
1000.0 μl of water (100 v/v)
F9NA
1000.0 μl of water (100 v/v)
F10NA
1000.0 μl of water (100 v/v)
F11NA
1000.0 μl of water (100 v/v)
F12NA
1000.0 μl of water (100 v/v)
G1NA
1000.0 μl of water (100 v/v)
G2NA
1000.0 μl of water (100 v/v)
G3NA
1000.0 μl of water (100 v/v)
G4NA
1000.0 μl of water (100 v/v)
G5NA
1000.0 μl of water (100 v/v)
G6NA
1000.0 μl of water (100 v/v)
G7NA
1000.0 μl of water (100 v/v)
G8NA
1000.0 μl of water (100 v/v)
G9NA
1000.0 μl of water (100 v/v)
G10NA
1000.0 μl of water (100 v/v)
G11NA
1000.0 μl of water (100 v/v)
G12NA
1000.0 μl of water (100 v/v)
H1NA
1000.0 μl of water (100 v/v)
H2NA
1000.0 μl of water (100 v/v)
H3NA
1000.0 μl of water (100 v/v)
H4NA
1000.0 μl of water (100 v/v)
H5NA
1000.0 μl of water (100 v/v)
H6NA
1000.0 μl of water (100 v/v)
H7NA
1000.0 μl of water (100 v/v)
H8NA
1000.0 μl of water (100 v/v)
H9NA
1000.0 μl of water (100 v/v)
H10NA
1000.0 μl of water (100 v/v)
H11NA
1000.0 μl of water (100 v/v)
H12NA
1000.0 μl of water (100 v/v)