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Screen Description

Precipitant Synergy 67%

Rigaku / Precipitant Synergy 67%
Created 2009-11-16.

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Precipitant Synergy 67%

    Screen Structure

    Precipitant Synergy 67%

    Well Count 96
    Number of distinct ingredients 24
    Internal diversity (score mean dist) 0.904

    Screen Composition

    2025-06-14T13:51:52.185936 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Precipitant Synergy 67%
    Buffer 66
    Cryoprotectant 2
    Organic (non-volatile) 37
    Organic (volatile) 18
    Polymer 56
    Salt 74

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Precipitant Synergy 67% Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium sulfate 1.34 M -
    • Polyethylene glycol 400 1.34 v/v -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    382 μl of Ammonium sulfate (3.5 M)
    503 μl of water (100 v/v)
    A2
    • Ammonium sulfate 1.34 M -
    • Glycerol 6.7 v/v -
    • Magnesium chloride 0.05 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    100 μl of Imidazole pH 6.5 (1 M)
    67 μl of Glycerol (100 v/v)
    382 μl of Ammonium sulfate (3.5 M)
    25 μl of Magnesium chloride (2 M)
    425 μl of water (100 v/v)
    A3
    • Ammonium sulfate 1.34 M -
    • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    382 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    510 μl of water (100 v/v)
    A4
    • Ammonium sulfate 1.34 M -
    • Polyethylene glycol 400 3.35 v/v -
    • Magnesium sulfate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    25 μl of Magnesium sulfate (2 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    382 μl of Ammonium sulfate (3.5 M)
    458 μl of water (100 v/v)
    A5
    • Sodium chloride 2.61 M -
    • Polyethylene glycol 400 1.34 v/v -
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    522 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    314 μl of water (100 v/v)
    A6
    • Sodium chloride 2.01 M -
    • 2-methyl-2,4-pentanediol BCC 3.35 v/v -
    • Calcium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    33 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    402 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    414 μl of water (100 v/v)
    A7
    • Sodium chloride 2.68 M -
    • 2-Propanol 3.35 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    33 μl of 2-Propanol (100 v/v)
    536 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    330 μl of water (100 v/v)
    A8
    • 2-Propanol 3.35 v/v -
    • Potassium Sodium phosphate 1.68 M pH 5.5
    pH 5.5
    33 μl of 2-Propanol (100 v/v)
    558 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    408 μl of water (100 v/v)
    A9
    • Polyethylene glycol 400 1.34 v/v -
    • Potassium Sodium phosphate 1.34 M pH 6.5
    pH 6.5
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    446 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    539 μl of water (100 v/v)
    A10
    • Glycerol 13.4 v/v -
    • Potassium Sodium phosphate 1.68 M pH 7.5
    pH 7.5
    558 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    134 μl of Glycerol (100 v/v)
    307 μl of water (100 v/v)
    A11
    • 2-methyl-2,4-pentanediol BCC 5.36 v/v -
    • Potassium Sodium phosphate 0.67 M pH 8.5
    pH 8.5
    53 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    335 μl of Potassium Sodium phosphate pH 8.5 (2 M)
    611 μl of water (100 v/v)
    A12
    • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
    • tri-Ammonium citrate 1.34 M pH 4.5
    pH 4.5
    6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    446 μl of tri-Ammonium citrate pH 4.5 (3 M)
    546 μl of water (100 v/v)
    B1
    • 2-Propanol 3.35 v/v -
    • tri-Ammonium citrate 1.34 M pH 6.5
    pH 6.5
    33 μl of 2-Propanol (100 v/v)
    442 μl of tri-Ammonium citrate pH 7.5 (3 M)
    4 μl of tri-Ammonium citrate pH 4.5 (3 M)
    519 μl of water (100 v/v)
    B2
    • Polyethylene glycol 400 3.35 v/v -
    • tri-Ammonium citrate 1.34 M pH 7.5
    pH 7.5
    446 μl of tri-Ammonium citrate pH 7.5 (3 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    519 μl of water (100 v/v)
    B3
    • Lithium sulfate 1.34 M -
    • 2-Propanol 3.35 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    33 μl of 2-Propanol (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    536 μl of Lithium sulfate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    280 μl of water (100 v/v)
    B4
    • Lithium sulfate 1.34 M -
    • Polyethylene glycol 400 3.35 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium sulfate (2 M)
    536 μl of Lithium sulfate (2.5 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    280 μl of water (100 v/v)
    B5
    • Lithium sulfate 1.14 M -
    • 2-methyl-2,4-pentanediol BCC 4.56 v/v -
    • Imidazole 0.085 M pH 6.5
    pH 6.5
    45 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    85 μl of Imidazole pH 6.5 (1 M)
    455 μl of Lithium sulfate (2.5 M)
    413 μl of water (100 v/v)
    B6
    • Lithium sulfate 1.34 M -
    • Polyethylene glycol 400 1.34 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    536 μl of Lithium sulfate (2.5 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of water (100 v/v)
    B7
    • Lithium sulfate 0.636 M -
    • 2-methyl-2,4-pentanediol BCC 9.55 v/v -
    • Magnesium sulfate 0.095 M -
    • Cadmium chloride 0 M -
    NA
    95 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    0 μl of Cadmium chloride (1 M)
    47 μl of Magnesium sulfate (2 M)
    254 μl of Lithium sulfate (2.5 M)
    602 μl of water (100 v/v)
    B8
    • 2-methyl-2,4-pentanediol BCC 15.1 v/v -
    • tri-Ammonium citrate 0.453 M pH 5.5
    pH 5.5
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    0 μl of tri-Ammonium citrate pH 7.5 (3 M)
    151 μl of tri-Ammonium citrate pH 4.5 (3 M)
    698 μl of water (100 v/v)
    B9
    • Ammonium sulfate 1 M -
    • 2-Propanol 8.04 v/v -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    80 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    287 μl of Ammonium sulfate (3.5 M)
    532 μl of water (100 v/v)
    B10
    • Sodium chloride 0.871 M -
    • 2-Propanol 20.1 v/v -
    • Calcium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 6.5
    pH 6.5
    100 μl of tri-Sodium citrate pH 6.5 (1 M)
    201 μl of 2-Propanol (100 v/v)
    174 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    474 μl of water (100 v/v)
    B11
    • Sodium chloride 2.68 M -
    • Polyethylene glycol 400 6.7 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    67 μl of Polyethylene glycol 400 (100 v/v)
    536 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    297 μl of water (100 v/v)
    B12
    • Polyethylene glycol 400 13.4 v/v -
    • Potassium Sodium phosphate 0.335 M pH 7.5
    pH 7.5
    111 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    134 μl of Polyethylene glycol 400 (100 v/v)
    754 μl of water (100 v/v)
    C1
    • 2-Propanol 10.1 v/v -
    • tri-Ammonium citrate 0.67 M pH 8.5
    pH 8.5
    100 μl of 2-Propanol (100 v/v)
    335 μl of tri-Ammonium citrate pH 8.5 (2 M)
    564 μl of water (100 v/v)
    C2
    • Sodium formate 1.34 M -
    • 2-Propanol 10.1 v/v -
    • Polyethylene glycol 3,350 1.68 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of 2-Propanol (100 v/v)
    33 μl of Polyethylene glycol 3,350 (50 w/v)
    191 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    574 μl of water (100 v/v)
    C3
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 1,500 16.8 w/v -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    335 μl of Polyethylene glycol 1,500 (50 w/v)
    364 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 8,000 10.1 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    201 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Calcium chloride (2 M)
    448 μl of water (100 v/v)
    C5
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 3,350 6.7 w/v -
    • Ammonium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    134 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    507 μl of water (100 v/v)
    C6
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Cadmium chloride 0 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    0 μl of Cadmium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    699 μl of water (100 v/v)
    C7
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • Polyethylene glycol 8,000 5.36 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    107 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Sodium chloride (5 M)
    492 μl of water (100 v/v)
    C8
    • 2-Propanol 20.1 v/v -
    • Polyethylene glycol 3,350 2.68 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    201 μl of 2-Propanol (100 v/v)
    53 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    50 μl of Calcium chloride (2 M)
    595 μl of water (100 v/v)
    C9
    • 2-Propanol 20.1 v/v -
    • Polyethylene glycol 1,500 6.7 w/v -
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    201 μl of 2-Propanol (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    134 μl of Polyethylene glycol 1,500 (50 w/v)
    485 μl of water (100 v/v)
    C10
    • 2-Propanol 26.8 v/v -
    • Polyethylene glycol 8,000 10.1 w/v -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    268 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    201 μl of Polyethylene glycol 8,000 (50 w/v)
    431 μl of water (100 v/v)
    C11
    • 2-Propanol 13.4 v/v -
    • Polyethylene glycol 3,350 10.1 w/v -
    • tri-Ammonium citrate 0.2 M pH 7.5
    pH 7.5
    134 μl of 2-Propanol (100 v/v)
    201 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of tri-Ammonium citrate pH 7.5 (3 M)
    598 μl of water (100 v/v)
    C12
    • 2-Propanol 20.1 v/v -
    • Polyethylene glycol 3,350 20.1 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    201 μl of 2-Propanol (100 v/v)
    402 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    297 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 26.8 v/v -
    • Polyethylene glycol 8,000 13.4 w/v -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    268 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    268 μl of Polyethylene glycol 400 (100 v/v)
    364 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 26.8 v/v -
    • Polyethylene glycol 3,350 3.35 w/v -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    67 μl of Polyethylene glycol 3,350 (50 w/v)
    268 μl of Polyethylene glycol 400 (100 v/v)
    565 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 13.4 v/v -
    • Polyethylene glycol 1,000 10.1 w/v -
    • Potassium Sodium phosphate 0.15 M pH 6.5
    pH 6.5
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    134 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    201 μl of Polyethylene glycol 1,000 (50 w/v)
    615 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 26.8 v/v -
    • Polyethylene glycol 8,000 5.36 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    107 μl of Polyethylene glycol 8,000 (50 w/v)
    268 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    524 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 16.8 v/v -
    • Polyethylene glycol 3,350 13.4 w/v -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    268 μl of Polyethylene glycol 3,350 (50 w/v)
    167 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    414 μl of water (100 v/v)
    D6
    • Polyethylene glycol 1,500 20.1 w/v -
    • 2-methyl-2,4-pentanediol BCC 2.01 v/v -
    • Magnesium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    20 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Magnesium sulfate (2 M)
    402 μl of Polyethylene glycol 1,500 (50 w/v)
    377 μl of water (100 v/v)
    D7
    • Polyethylene glycol 1,500 20.1 w/v -
    • 2-Propanol 6.7 v/v -
    • Calcium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    67 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    402 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Calcium chloride (2 M)
    381 μl of water (100 v/v)
    D8
    • Polyethylene glycol 1,500 20.1 w/v -
    • Polyethylene glycol 400 13.4 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    134 μl of Polyethylene glycol 400 (100 v/v)
    402 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    364 μl of water (100 v/v)
    D9
    • Polyethylene glycol 1,500 20.1 w/v -
    • 2-methyl-2,4-pentanediol BCC 5.36 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    53 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    402 μl of Polyethylene glycol 1,500 (50 w/v)
    444 μl of water (100 v/v)
    D10
    • Polyethylene glycol 3,350 16.8 w/v -
    • 2-Propanol 10.1 v/v -
    • tri-Ammonium citrate 0.2 M pH 4.5
    pH 4.5
    100 μl of 2-Propanol (100 v/v)
    335 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of tri-Ammonium citrate pH 4.5 (3 M)
    497 μl of water (100 v/v)
    D11
    • Polyethylene glycol 3,350 16.8 w/v -
    • Polyethylene glycol 400 3.35 v/v -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    335 μl of Polyethylene glycol 3,350 (50 w/v)
    33 μl of Polyethylene glycol 400 (100 v/v)
    531 μl of water (100 v/v)
    D12
    • Polyethylene glycol 3,350 16.8 w/v -
    • 2-methyl-2,4-pentanediol BCC 10.1 v/v -
    • Lithium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    335 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    384 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,350 16.8 w/v -
    • 2-Propanol 2.68 v/v -
    • Calcium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    26 μl of 2-Propanol (100 v/v)
    335 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Calcium chloride (2 M)
    488 μl of water (100 v/v)
    E2
    • Polyethylene glycol 8,000 13.4 w/v -
    • Polyethylene glycol 400 6.7 v/v -
    • Sodium chloride 0.5 M -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    268 μl of Polyethylene glycol 8,000 (50 w/v)
    67 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (5 M)
    465 μl of water (100 v/v)
    E3
    • Polyethylene glycol 8,000 13.4 w/v -
    • 2-methyl-2,4-pentanediol BCC 2.01 v/v -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    20 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    268 μl of Polyethylene glycol 8,000 (50 w/v)
    611 μl of water (100 v/v)
    E4
    • Polyethylene glycol 8,000 13.4 w/v -
    • 2-Propanol 6.7 v/v -
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    67 μl of 2-Propanol (100 v/v)
    268 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    507 μl of water (100 v/v)
    E5
    • Polyethylene glycol 8,000 13.4 w/v -
    • Polyethylene glycol 400 13.4 v/v -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    268 μl of Polyethylene glycol 8,000 (50 w/v)
    134 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    448 μl of water (100 v/v)
    E6
    • Sodium formate 1.41 M -
    • Polyethylene glycol 3,350 16.8 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    335 μl of Polyethylene glycol 3,350 (50 w/v)
    201 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    50 μl of Calcium chloride (2 M)
    314 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.502 M -
    • Polyethylene glycol 3,350 5.03 w/v -
    • 2-Propanol 3.35 v/v -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    33 μl of 2-Propanol (100 v/v)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    143 μl of Ammonium sulfate (3.5 M)
    622 μl of water (100 v/v)
    E8
    • Polyethylene glycol 4,000 0.67 w/v -
    • tri-Ammonium citrate 0.67 M pH 5.5
    pH 5.5
    0 μl of tri-Ammonium citrate pH 7.5 (3 M)
    13 μl of Polyethylene glycol 4,000 (50 w/v)
    223 μl of tri-Ammonium citrate pH 4.5 (3 M)
    763 μl of water (100 v/v)
    E9
    • Sodium chloride 1.68 M -
    • Polyethylene glycol 1,500 8.04 w/v -
    • 2-methyl-2,4-pentanediol BCC 1 v/v -
    • Sodium acetate 0.1 M pH 5.5
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    10 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    335 μl of Sodium chloride (5 M)
    160 μl of Polyethylene glycol 1,500 (50 w/v)
    394 μl of water (100 v/v)
    E10
    • Sodium chloride 1.34 M -
    • Polyethylene glycol 3,350 13.4 w/v -
    • Magnesium chloride 0.1 M -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    268 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    268 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    314 μl of water (100 v/v)
    E11
    • Sodium formate 2.01 M -
    • Polyethylene glycol 8,000 2.68 w/v -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    100 μl of Imidazole pH 6.5 (1 M)
    53 μl of Polyethylene glycol 8,000 (50 w/v)
    287 μl of Sodium formate (7 M)
    559 μl of water (100 v/v)
    E12
    • Polyethylene glycol 4,000 0.34 w/v -
    • Potassium Sodium phosphate 0.67 M pH 7.5
    pH 7.5
    223 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    6 μl of Polyethylene glycol 4,000 (50 w/v)
    769 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 7.82 w/v -
    • Potassium Sodium phosphate 0.335 M pH 7.5
    pH 7.5
    156 μl of Polyethylene glycol 3,350 (50 w/v)
    111 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    731 μl of water (100 v/v)
    F2
    • Polyethylene glycol 8,000 1.34 w/v -
    • tri-Ammonium citrate 0.335 M pH 8.5
    pH 8.5
    26 μl of Polyethylene glycol 8,000 (50 w/v)
    167 μl of tri-Ammonium citrate pH 8.5 (2 M)
    805 μl of water (100 v/v)
    F3
    • Sodium chloride 1.34 M -
    • Polyethylene glycol 4,000 3.35 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    67 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    268 μl of Sodium chloride (5 M)
    565 μl of water (100 v/v)
    F4
    • Polyethylene glycol 8,000 10.1 w/v -
    • tri-Ammonium citrate 0.335 M pH 8.5
    pH 8.5
    201 μl of Polyethylene glycol 8,000 (50 w/v)
    167 μl of tri-Ammonium citrate pH 8.5 (2 M)
    631 μl of water (100 v/v)
    F5NA
    F6NA
    F7NA
    F8NA
    F9NA
    F10NA
    F11NA
    F12NA
    G1NA
    G2NA
    G3NA
    G4NA
    G5NA
    G6NA
    G7NA
    G8NA
    G9NA
    G10NA
    G11NA
    G12NA
    H1NA
    H2NA
    H3NA
    H4NA
    H5NA
    H6NA
    H7NA
    H8NA
    H9NA
    H10NA
    H11NA
    H12NA