C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard I

Rigaku / Wizard I

Created 2009-11-16.

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Wizard I

Barcodes

Wizard I

Screen Structure

Wizard I

Well Count	48
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.943

Screen Composition

More information about screen composition chart.

ingredient type	Wizard I
Buffer	58
Organic (non-volatile)	6
Organic (volatile)	8
Polymer	22
Salt	58

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard I Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 20 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v)
A2	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	40 μl of Sodium chloride (5 M) 100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 760 μl of water (100 v/v)
A3	Ethanol 15 v/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	150 μl of Ethanol (100 v/v) 100 μl of CHES pH 9.5 (1 M) 750 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 35 v/v - Imidazole 0.1 M pH 8 Magnesium chloride 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of Magnesium chloride (2 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 450 μl of water (100 v/v)
A5	Polyethylene glycol 400 30 v/v - CAPS 0.1 M pH 10 🔍	pH 10	300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of CAPS pH 10 (1 M) 600 μl of water (100 v/v)
A6	Polyethylene glycol 3,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	25 μl of tri-Sodium citrate pH 5.5 (4 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 575 μl of water (100 v/v)
A7	Polyethylene glycol 8,000 10 w/v - MES 0.1 M pH 6 Zinc acetate 0.2 M - 🔍	pH 6	83 μl of MES pH 7.1 (0.5 M) 117 μl of MES pH 5.2 (0.5 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Zinc acetate (1 M) 400 μl of water (100 v/v)
A8	Ammonium sulfate 2 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	571 μl of Ammonium sulfate (3.5 M) 25 μl of tri-Sodium citrate pH 5.5 (4 M) 403 μl of water (100 v/v)
B1	di-Ammonium hydrogen phosphate 1 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	285 μl of di-Ammonium hydrogen phosphate (3.5 M) 33 μl of Sodium acetate pH 4.5 (3 M) 681 μl of water (100 v/v)
B2	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 7 (1 M) 500 μl of water (100 v/v)
B3	1,4-Butanediol 20 v/v - MES 0.1 M pH 6 Lithium sulfate 0.2 M - 🔍	pH 6	83 μl of MES pH 7.1 (0.5 M) 200 μl of 1,4-Butanediol (100 v/v) 117 μl of MES pH 5.2 (0.5 M) 80 μl of Lithium sulfate (2.5 M) 520 μl of water (100 v/v)
B4	Polyethylene glycol 1,000 20 w/v - Imidazole 0.1 M pH 8 Calcium acetate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 200 μl of Calcium acetate (1 M) 300 μl of water (100 v/v)
B5	Ammonium sulfate 1.26 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	360 μl of Ammonium sulfate (3.5 M) 31 μl of Sodium cacodylate pH 5 (1 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 540 μl of water (100 v/v)
B6	tri-Sodium citrate 1 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	31 μl of Sodium cacodylate pH 5 (1 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 625 μl of tri-Sodium citrate (1.6 M) 275 μl of water (100 v/v)
B7	Polyethylene glycol 3,000 10 w/v - Imidazole 0.1 M pH 8 Lithium sulfate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 80 μl of Lithium sulfate (2.5 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 620 μl of water (100 v/v)
B8	Sodium chloride 2.5 M - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	500 μl of Sodium chloride (5 M) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 475 μl of water (100 v/v)
C1	Polyethylene glycol 8,000 30 w/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80 μl of Lithium sulfate (2.5 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of Sodium acetate pH 4.5 (3 M) 286 μl of water (100 v/v)
C2	Potassium Sodium tartrate 1 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	40 μl of Sodium chloride (5 M) 100 μl of Imidazole pH 8 (1 M) 666 μl of Potassium Sodium tartrate (1.5 M) 193 μl of water (100 v/v)
C3	Polyethylene glycol 1,000 20 w/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	100 μl of tris chloride BCC pH 7 (1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 500 μl of water (100 v/v)
C4	Sodium di-hydrogen phosphate 0.4 M - di-Potassium hydrogen phosphate 1.6 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	40 μl of Sodium chloride (5 M) 100 μl of Imidazole pH 8 (1 M) 80 μl of Sodium di-hydrogen phosphate (5 M) 533 μl of di-Potassium hydrogen phosphate (3 M) 246 μl of water (100 v/v)
C5	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v)
C6	2-Propanol 10 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of 2-Propanol (100 v/v) 800 μl of water (100 v/v)
C7	Ethanol 15 v/v - Imidazole 0.1 M pH 8 Magnesium chloride 0.2 M - 🔍	pH 8	150 μl of Ethanol (100 v/v) 100 μl of Imidazole pH 8 (1 M) 100 μl of Magnesium chloride (2 M) 650 μl of water (100 v/v)
C8	2-methyl-2,4-pentanediol BCC 35 v/v - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	40 μl of Sodium chloride (5 M) 100 μl of tris chloride BCC pH 7 (1 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 510 μl of water (100 v/v)
D1	Polyethylene glycol 400 30 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Magnesium chloride (2 M) 500 μl of water (100 v/v)
D2	Polyethylene glycol 3,000 10 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 700 μl of water (100 v/v)
D3	Sodium di-hydrogen phosphate 1.2 M - di-Potassium hydrogen phosphate 0.8 M - CAPS 0.1 M pH 10 Lithium sulfate 0.2 M - 🔍	pH 10	80 μl of Lithium sulfate (2.5 M) 100 μl of CAPS pH 10 (1 M) 240 μl of Sodium di-hydrogen phosphate (5 M) 266 μl of di-Potassium hydrogen phosphate (3 M) 313 μl of water (100 v/v)
D4	Polyethylene glycol 3,000 20 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	40 μl of Sodium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 460 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 10 w/v - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	40 μl of Sodium chloride (5 M) 100 μl of CHES pH 9.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 660 μl of water (100 v/v)
D6	Ammonium sulfate 1.26 M - Sodium acetate 0.1 M pH 4.5 Sodium chloride 0.2 M - 🔍	pH 4.5	40 μl of Sodium chloride (5 M) 360 μl of Ammonium sulfate (3.5 M) 33 μl of Sodium acetate pH 4.5 (3 M) 566 μl of water (100 v/v)
D7	Polyethylene glycol 8,000 20 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	40 μl of Sodium chloride (5 M) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v)
D8	Polyethylene glycol 3,000 10 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 775 μl of water (100 v/v)
E1	Ammonium sulfate 2 M - CAPS 0.1 M pH 10 Lithium sulfate 0.2 M - 🔍	pH 10	571 μl of Ammonium sulfate (3.5 M) 80 μl of Lithium sulfate (2.5 M) 100 μl of CAPS pH 10 (1 M) 248 μl of water (100 v/v)
E2	di-Ammonium hydrogen phosphate 1 M - Imidazole 0.1 M pH 8 🔍	pH 8	285 μl of di-Ammonium hydrogen phosphate (3.5 M) 100 μl of Imidazole pH 8 (1 M) 614 μl of water (100 v/v)
E3	1,4-Butanediol 20 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200 μl of 1,4-Butanediol (100 v/v) 33 μl of Sodium acetate pH 4.5 (3 M) 766 μl of water (100 v/v)
E4	tri-Sodium citrate 1 M - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 625 μl of tri-Sodium citrate (1.6 M) 275 μl of water (100 v/v)
E5	Sodium chloride 2.5 M - Imidazole 0.1 M pH 8 🔍	pH 8	500 μl of Sodium chloride (5 M) 100 μl of Imidazole pH 8 (1 M) 400 μl of water (100 v/v)
E6	Potassium Sodium tartrate 1 M - CHES 0.1 M pH 9.5 Lithium sulfate 0.2 M - 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 80 μl of Lithium sulfate (2.5 M) 666 μl of Potassium Sodium tartrate (1.5 M) 153 μl of water (100 v/v)
E7	Polyethylene glycol 1,000 20 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Lithium sulfate 0.2 M - 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 80 μl of Lithium sulfate (2.5 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 420 μl of water (100 v/v)
E8	2-Propanol 10 v/v - MES 0.1 M pH 6 Calcium acetate 0.2 M - 🔍	pH 6	83 μl of MES pH 7.1 (0.5 M) 100 μl of 2-Propanol (100 v/v) 117 μl of MES pH 5.2 (0.5 M) 200 μl of Calcium acetate (1 M) 500 μl of water (100 v/v)
F1	Polyethylene glycol 3,000 30 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 600 μl of Polyethylene glycol 3,000 (50 w/v) 300 μl of water (100 v/v)
F2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	150 μl of Ethanol (100 v/v) 100 μl of tris chloride BCC pH 7 (1 M) 750 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 625 μl of water (100 v/v)
F4	Polyethylene glycol 400 30 v/v - Sodium acetate 0.1 M pH 4.5 Calcium acetate 0.2 M - 🔍	pH 4.5	300 μl of Polyethylene glycol 400 (100 v/v) 33 μl of Sodium acetate pH 4.5 (3 M) 200 μl of Calcium acetate (1 M) 466 μl of water (100 v/v)
F5	Polyethylene glycol 3,000 20 w/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 566 μl of water (100 v/v)
F6	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 Calcium acetate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Calcium acetate (1 M) 500 μl of water (100 v/v)
F7	Ammonium sulfate 1.26 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 360 μl of Ammonium sulfate (3.5 M) 80 μl of Lithium sulfate (2.5 M) 460 μl of water (100 v/v)
F8	Polyethylene glycol 1,000 20 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	400 μl of Polyethylene glycol 1,000 (50 w/v) 33 μl of Sodium acetate pH 4.5 (3 M) 200 μl of Zinc acetate (1 M) 366 μl of water (100 v/v)