C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard II

Rigaku / Wizard II

Created 2009-11-16.

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Wizard II

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Wizard II

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Screen Structure

Wizard II

Well Count	48
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.929

Screen Composition

More information about screen composition chart.

ingredient type	Wizard II
Buffer	56
Organic (non-volatile)	6
Organic (volatile)	8
Polymer	19
Salt	72

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard II Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,000 10 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	200.0 μl of Polyethylene glycol 3,000 (50 w/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 200.0 μl of Zinc acetate (1 M) 566.7 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 35 v/v - MES 0.1 M pH 6 Lithium sulfate 0.2 M - 🔍	pH 6	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Lithium sulfate (2.5 M) 117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 370.0 μl of water (100 v/v)
A3	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
A4	Ammonium sulfate 2 M - Sodium cacodylate 0.1 M pH 6.5 Sodium chloride 0.2 M - 🔍	pH 6.5	571.4 μl of Ammonium sulfate (3.5 M) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 40.0 μl of Sodium chloride (5 M) 288.6 μl of water (100 v/v)
A5	1,4-Butanediol 20 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	200.0 μl of 1,4-Butanediol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 660.0 μl of water (100 v/v)
A6	2-Propanol 10 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Lithium sulfate 0.2 M - 🔍	pH 4.2	100.0 μl of 2-Propanol (100 v/v) 80.0 μl of Lithium sulfate (2.5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 720.0 μl of water (100 v/v)
A7	Polyethylene glycol 3,000 30 w/v - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	600.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 260.0 μl of water (100 v/v)
A8	Polyethylene glycol 8,000 10 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	200.0 μl of Polyethylene glycol 8,000 (50 w/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40.0 μl of Sodium chloride (5 M) 735.0 μl of water (100 v/v)
B1	Ammonium sulfate 2 M - Sodium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 328.6 μl of water (100 v/v)
B2	di-Ammonium hydrogen phosphate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 614.3 μl of water (100 v/v)
B3	2-Propanol 10 v/v - Sodium cacodylate 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	100.0 μl of 2-Propanol (100 v/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 200.0 μl of Zinc acetate (1 M) 600.0 μl of water (100 v/v)
B4	Polyethylene glycol 400 30 v/v - Sodium cacodylate 0.1 M pH 6.5 Lithium sulfate 0.2 M - 🔍	pH 6.5	80.0 μl of Lithium sulfate (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 520.0 μl of water (100 v/v)
B5	Ethanol 15 v/v - tri-Sodium citrate 0.1 M pH 5.5 Lithium sulfate 0.2 M - 🔍	pH 5.5	150.0 μl of Ethanol (100 v/v) 80.0 μl of Lithium sulfate (2.5 M) 25.0 μl of tri-Sodium citrate pH 5.5 (4 M) 745.0 μl of water (100 v/v)
B6	Polyethylene glycol 1,000 20 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	400.0 μl of Polyethylene glycol 1,000 (50 w/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40.0 μl of Sodium chloride (5 M) 535.0 μl of water (100 v/v)
B7	Ammonium sulfate 1.26 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	360.0 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 540.0 μl of water (100 v/v)
B8	tri-Sodium citrate 1 M - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v)
C1	Sodium chloride 2.5 M - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	100.0 μl of Magnesium chloride (2 M) 500.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 300.0 μl of water (100 v/v)
C2	Polyethylene glycol 3,000 20 w/v - tris chloride BCC 0.1 M pH 7 Calcium acetate 0.2 M - 🔍	pH 7	200.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 300.0 μl of water (100 v/v)
C3	Sodium di-hydrogen phosphate 1.6 M - Sodium phosphate citrate 0.1 M pH 4.2 di-Potassium hydrogen phosphate 0.4 M - 🔍	pH 4.2	320.0 μl of Sodium di-hydrogen phosphate (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 133.3 μl of di-Potassium hydrogen phosphate (3 M) 446.7 μl of water (100 v/v)
C4	Ethanol 15 v/v - MES 0.1 M pH 6 Zinc acetate 0.2 M - 🔍	pH 6	150.0 μl of Ethanol (100 v/v) 117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 200.0 μl of Zinc acetate (1 M) 450.0 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 616.7 μl of water (100 v/v)
C6	2-Propanol 10 v/v - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 800.0 μl of water (100 v/v)
C7	Ethanol 15 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	150.0 μl of Ethanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 650.0 μl of water (100 v/v)
C8	Polyethylene glycol 8,000 30 w/v - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 260.0 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 35 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 510.0 μl of water (100 v/v)
D2	Polyethylene glycol 400 30 v/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 600.0 μl of water (100 v/v)
D3	Polyethylene glycol 3,000 10 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	100.0 μl of Magnesium chloride (2 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 600.0 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 20 w/v - MES 0.1 M pH 6 Calcium acetate 0.2 M - 🔍	pH 6	200.0 μl of Calcium acetate (1 M) 117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of water (100 v/v)
D5	Ammonium sulfate 1.26 M - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	360.0 μl of Ammonium sulfate (3.5 M) 100.0 μl of CHES pH 9.5 (1 M) 40.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
D6	1,4-Butanediol 20 v/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	200.0 μl of 1,4-Butanediol (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Zinc acetate (1 M) 500.0 μl of water (100 v/v)
D7	tri-Sodium citrate 1 M - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	40.0 μl of Sodium chloride (5 M) 625.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 235.0 μl of water (100 v/v)
D8	Polyethylene glycol 1,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
E1	di-Ammonium hydrogen phosphate 1 M - tri-Sodium citrate 0.1 M pH 5.5 Sodium chloride 0.2 M - 🔍	pH 5.5	40.0 μl of Sodium chloride (5 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 25.0 μl of tri-Sodium citrate pH 5.5 (4 M) 649.3 μl of water (100 v/v)
E2	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v)
E3	Sodium di-hydrogen phosphate 0.8 M - Potassium di-hydrogen phosphate 1.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	800.0 μl of Potassium di-hydrogen phosphate (1.5 M) 33.3 μl of Sodium acetate pH 4.5 (3 M) 160.0 μl of Sodium di-hydrogen phosphate (5 M) 6.7 μl of water (100 v/v)
E4	Polyethylene glycol 3,000 10 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	200.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 660.0 μl of water (100 v/v)
E5	Potassium Sodium tartrate 1 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	80.0 μl of Lithium sulfate (2.5 M) 666.7 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 153.3 μl of water (100 v/v)
E6	Sodium chloride 2.5 M - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80.0 μl of Lithium sulfate (2.5 M) 33.3 μl of Sodium acetate pH 4.5 (3 M) 500.0 μl of Sodium chloride (5 M) 386.7 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 20 w/v - CAPS 0.1 M pH 10 Sodium chloride 0.2 M - 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 460.0 μl of water (100 v/v)
E8	Polyethylene glycol 3,000 20 w/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 200.0 μl of Zinc acetate (1 M) 300.0 μl of water (100 v/v)
F1	Ammonium sulfate 2 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	571.4 μl of Ammonium sulfate (3.5 M) 80.0 μl of Lithium sulfate (2.5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 248.6 μl of water (100 v/v)
F2	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 40.0 μl of Sodium chloride (5 M) 560.0 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	100.0 μl of Magnesium chloride (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 600.0 μl of water (100 v/v)
F4	Polyethylene glycol 1,000 20 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 400.0 μl of water (100 v/v)
F5	Ammonium sulfate 1.26 M - MES 0.1 M pH 6 🔍	pH 6	360.0 μl of Ammonium sulfate (3.5 M) 117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 440.0 μl of water (100 v/v)
F6	di-Ammonium hydrogen phosphate 1 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 40.0 μl of Sodium chloride (5 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 574.3 μl of water (100 v/v)
F7	Sodium chloride 2.5 M - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium chloride (5 M) 200.0 μl of Zinc acetate (1 M) 200.0 μl of water (100 v/v)
F8	Potassium Sodium tartrate 1 M - MES 0.1 M pH 6 🔍	pH 6	117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 666.7 μl of Potassium Sodium tartrate (1.5 M) 133.3 μl of water (100 v/v)