C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard II

Rigaku / Wizard II

Created 2009-11-16.

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Wizard II

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Wizard II

Screen Structure

Wizard II

Well Count	48
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.929

Screen Composition

More information about screen composition chart.

ingredient type	Wizard II
Buffer	56
Organic (non-volatile)	6
Organic (volatile)	8
Polymer	19
Salt	72

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard II Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,000 10 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	200 μl of Zinc acetate (1 M) 33 μl of Sodium acetate pH 4.5 (3 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 566 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 35 v/v - MES 0.1 M pH 6 Lithium sulfate 0.2 M - 🔍	pH 6	117 μl of MES pH 5.2 (0.5 M) 80 μl of Lithium sulfate (2.5 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 83 μl of MES pH 7.1 (0.5 M) 370 μl of water (100 v/v)
A3	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium chloride (2 M) 400 μl of water (100 v/v)
A4	Ammonium sulfate 2 M - Sodium cacodylate 0.1 M pH 6.5 Sodium chloride 0.2 M - 🔍	pH 6.5	31 μl of Sodium cacodylate pH 5 (1 M) 40 μl of Sodium chloride (5 M) 571 μl of Ammonium sulfate (3.5 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 288 μl of water (100 v/v)
A5	1,4-Butanediol 20 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Sodium chloride (5 M) 200 μl of 1,4-Butanediol (100 v/v) 660 μl of water (100 v/v)
A6	2-Propanol 10 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Lithium sulfate 0.2 M - 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 100 μl of 2-Propanol (100 v/v) 80 μl of Lithium sulfate (2.5 M) 720 μl of water (100 v/v)
A7	Polyethylene glycol 3,000 30 w/v - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	100 μl of tris chloride BCC pH 7 (1 M) 600 μl of Polyethylene glycol 3,000 (50 w/v) 40 μl of Sodium chloride (5 M) 260 μl of water (100 v/v)
A8	Polyethylene glycol 8,000 10 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	200 μl of Polyethylene glycol 8,000 (50 w/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40 μl of Sodium chloride (5 M) 735 μl of water (100 v/v)
B1	Ammonium sulfate 2 M - Sodium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v)
B2	di-Ammonium hydrogen phosphate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 614 μl of water (100 v/v)
B3	2-Propanol 10 v/v - Sodium cacodylate 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	200 μl of Zinc acetate (1 M) 100 μl of 2-Propanol (100 v/v) 31 μl of Sodium cacodylate pH 5 (1 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 600 μl of water (100 v/v)
B4	Polyethylene glycol 400 30 v/v - Sodium cacodylate 0.1 M pH 6.5 Lithium sulfate 0.2 M - 🔍	pH 6.5	300 μl of Polyethylene glycol 400 (100 v/v) 80 μl of Lithium sulfate (2.5 M) 31 μl of Sodium cacodylate pH 5 (1 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 520 μl of water (100 v/v)
B5	Ethanol 15 v/v - tri-Sodium citrate 0.1 M pH 5.5 Lithium sulfate 0.2 M - 🔍	pH 5.5	150 μl of Ethanol (100 v/v) 80 μl of Lithium sulfate (2.5 M) 25 μl of tri-Sodium citrate pH 5.5 (4 M) 745 μl of water (100 v/v)
B6	Polyethylene glycol 1,000 20 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	400 μl of Polyethylene glycol 1,000 (50 w/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40 μl of Sodium chloride (5 M) 535 μl of water (100 v/v)
B7	Ammonium sulfate 1.26 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 360 μl of Ammonium sulfate (3.5 M) 540 μl of water (100 v/v)
B8	tri-Sodium citrate 1 M - CHES 0.1 M pH 9.5 🔍	pH 9.5	625 μl of tri-Sodium citrate (1.6 M) 100 μl of CHES pH 9.5 (1 M) 275 μl of water (100 v/v)
C1	Sodium chloride 2.5 M - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	100 μl of tris chloride BCC pH 7 (1 M) 500 μl of Sodium chloride (5 M) 100 μl of Magnesium chloride (2 M) 300 μl of water (100 v/v)
C2	Polyethylene glycol 3,000 20 w/v - tris chloride BCC 0.1 M pH 7 Calcium acetate 0.2 M - 🔍	pH 7	200 μl of Calcium acetate (1 M) 100 μl of tris chloride BCC pH 7 (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 300 μl of water (100 v/v)
C3	Sodium di-hydrogen phosphate 1.6 M - Sodium phosphate citrate 0.1 M pH 4.2 di-Potassium hydrogen phosphate 0.4 M - 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 320 μl of Sodium di-hydrogen phosphate (5 M) 133 μl of di-Potassium hydrogen phosphate (3 M) 446 μl of water (100 v/v)
C4	Ethanol 15 v/v - MES 0.1 M pH 6 Zinc acetate 0.2 M - 🔍	pH 6	200 μl of Zinc acetate (1 M) 150 μl of Ethanol (100 v/v) 117 μl of MES pH 5.2 (0.5 M) 83 μl of MES pH 7.1 (0.5 M) 450 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 616 μl of water (100 v/v)
C6	2-Propanol 10 v/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of 2-Propanol (100 v/v) 800 μl of water (100 v/v)
C7	Ethanol 15 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	150 μl of Ethanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Magnesium chloride (2 M) 650 μl of water (100 v/v)
C8	Polyethylene glycol 8,000 30 w/v - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Sodium chloride (5 M) 260 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 35 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Sodium chloride (5 M) 510 μl of water (100 v/v)
D2	Polyethylene glycol 400 30 v/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of CHES pH 9.5 (1 M) 600 μl of water (100 v/v)
D3	Polyethylene glycol 3,000 10 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	31 μl of Sodium cacodylate pH 5 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of Magnesium chloride (2 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 600 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 20 w/v - MES 0.1 M pH 6 Calcium acetate 0.2 M - 🔍	pH 6	117 μl of MES pH 5.2 (0.5 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Calcium acetate (1 M) 83 μl of MES pH 7.1 (0.5 M) 200 μl of water (100 v/v)
D5	Ammonium sulfate 1.26 M - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	40 μl of Sodium chloride (5 M) 360 μl of Ammonium sulfate (3.5 M) 100 μl of CHES pH 9.5 (1 M) 500 μl of water (100 v/v)
D6	1,4-Butanediol 20 v/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	200 μl of Zinc acetate (1 M) 100 μl of Imidazole pH 8 (1 M) 200 μl of 1,4-Butanediol (100 v/v) 500 μl of water (100 v/v)
D7	tri-Sodium citrate 1 M - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	625 μl of tri-Sodium citrate (1.6 M) 100 μl of tris chloride BCC pH 7 (1 M) 40 μl of Sodium chloride (5 M) 235 μl of water (100 v/v)
D8	Polyethylene glycol 1,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
E1	di-Ammonium hydrogen phosphate 1 M - tri-Sodium citrate 0.1 M pH 5.5 Sodium chloride 0.2 M - 🔍	pH 5.5	40 μl of Sodium chloride (5 M) 25 μl of tri-Sodium citrate pH 5.5 (4 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 649 μl of water (100 v/v)
E2	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v)
E3	Sodium di-hydrogen phosphate 0.8 M - Potassium di-hydrogen phosphate 1.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 800 μl of Potassium di-hydrogen phosphate (1.5 M) 160 μl of Sodium di-hydrogen phosphate (5 M) 6 μl of water (100 v/v)
E4	Polyethylene glycol 3,000 10 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 40 μl of Sodium chloride (5 M) 660 μl of water (100 v/v)
E5	Potassium Sodium tartrate 1 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	666 μl of Potassium Sodium tartrate (1.5 M) 80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 7 (1 M) 153 μl of water (100 v/v)
E6	Sodium chloride 2.5 M - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 80 μl of Lithium sulfate (2.5 M) 500 μl of Sodium chloride (5 M) 386 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 20 w/v - CAPS 0.1 M pH 10 Sodium chloride 0.2 M - 🔍	pH 10	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of CAPS pH 10 (1 M) 40 μl of Sodium chloride (5 M) 460 μl of water (100 v/v)
E8	Polyethylene glycol 3,000 20 w/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	200 μl of Zinc acetate (1 M) 100 μl of Imidazole pH 8 (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 300 μl of water (100 v/v)
F1	Ammonium sulfate 2 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 7 (1 M) 571 μl of Ammonium sulfate (3.5 M) 248 μl of water (100 v/v)
F2	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 40 μl of Sodium chloride (5 M) 560 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 7 (1 M) 100 μl of Magnesium chloride (2 M) 600 μl of water (100 v/v)
F4	Polyethylene glycol 1,000 20 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	400 μl of Polyethylene glycol 1,000 (50 w/v) 31 μl of Sodium cacodylate pH 5 (1 M) 100 μl of Magnesium chloride (2 M) 68 μl of Sodium cacodylate pH 7.4 (1 M) 400 μl of water (100 v/v)
F5	Ammonium sulfate 1.26 M - MES 0.1 M pH 6 🔍	pH 6	117 μl of MES pH 5.2 (0.5 M) 360 μl of Ammonium sulfate (3.5 M) 83 μl of MES pH 7.1 (0.5 M) 440 μl of water (100 v/v)
F6	di-Ammonium hydrogen phosphate 1 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 40 μl of Sodium chloride (5 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 574 μl of water (100 v/v)
F7	Sodium chloride 2.5 M - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	200 μl of Zinc acetate (1 M) 100 μl of Imidazole pH 8 (1 M) 500 μl of Sodium chloride (5 M) 200 μl of water (100 v/v)
F8	Potassium Sodium tartrate 1 M - MES 0.1 M pH 6 🔍	pH 6	666 μl of Potassium Sodium tartrate (1.5 M) 117 μl of MES pH 5.2 (0.5 M) 83 μl of MES pH 7.1 (0.5 M) 133 μl of water (100 v/v)