C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard III

Rigaku / Wizard III

Created 2009-11-16.

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Wizard III

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Wizard III

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Screen Structure

Wizard III

Well Count	48
Number of distinct ingredients	45
Internal diversity (score mean dist)	0.954

Screen Composition

More information about screen composition chart.

ingredient type	Wizard III
Buffer	45
Cryoprotectant	6
Organic (non-volatile)	17
Organic (volatile)	5
Polymer	34
Salt	42

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard III Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,350 20 w/v - di-Ammonium hydrogen citrate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of di-Ammonium hydrogen citrate (2.5 M) 520.0 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.02 M - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 19.8 μl of Sodium acetate pH 3.6 (3 M) 13.6 μl of Sodium acetate pH 5.6 (3 M) 656.7 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - Magnesium formate 0.2 M - 🔍	NA	200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
A4	Polyethylene glycol 3,350 20 w/v - Ammonium formate 0.2 M - 🔍	NA	20.0 μl of Ammonium formate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v)
A5	Polyethylene glycol 3,350 20 w/v - Ammonium chloride 0.2 M - 🔍	NA	40.0 μl of Ammonium chloride (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v)
A6	Polyethylene glycol 3,350 20 w/v - Potassium formate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 14.3 μl of Potassium formate (14 M) 585.7 μl of water (100 v/v)
A7	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 0.2 M - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
A8	Polyethylene glycol 3,350 20 w/v - Potassium nitrate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate (3 M) 533.3 μl of water (100 v/v)
B1	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	228.6 μl of Ammonium sulfate (3.5 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 671.4 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 20 w/v - Sodium thiocyanate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate (8 M) 575.0 μl of water (100 v/v)
B3	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 10 w/v - HEPES 0.1 M pH 7.5 Ethylene glycol 8 v/v - 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 8 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	160.0 μl of Polyethylene glycol 4,000 (50 w/v) 19.8 μl of Sodium acetate pH 3.6 (3 M) 13.6 μl of Sodium acetate pH 5.6 (3 M) 806.7 μl of water (100 v/v)
B6	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v)
B7	tri-Sodium citrate 1.6 M - 🔍	NA	400.0 μl of tri-Sodium citrate (4 M) 600.0 μl of water (100 v/v)
B8	Polyethylene glycol 3,350 20 w/v - tri-Potassium citrate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of tri-Potassium citrate (1.5 M) 466.7 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 2-Propanol 10 v/v - 🔍	pH 5.5	100.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 72.6 μl of tri-Sodium citrate pH 3 (0.5 M) 127.4 μl of tri-Sodium citrate pH 6 (0.5 M) 300.0 μl of water (100 v/v)
C2	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 4 Lithium chloride 1 M - 🔍	pH 4	100.0 μl of Lithium chloride (10 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 400.0 μl of water (100 v/v)
C3	Polyethylene glycol 3,350 20 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20.0 μl of Ammonium nitrate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v)
C4	Polyethylene glycol 6,000 10 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v)
C5	Sodium Potassium phosphate 1.6 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 533.3 μl of Sodium Potassium phosphate (3 M) 366.7 μl of water (100 v/v)
C6	Ethanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	200.0 μl of Ethanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v)
C7	Polyethylene glycol 20,000 10 w/v - BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - 🔍	pH 9	20.0 μl of 1,4-Dioxane (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 546.7 μl of water (100 v/v)
C8	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	571.4 μl of Ammonium sulfate (3.5 M) 19.8 μl of Sodium acetate pH 3.6 (3 M) 13.6 μl of Sodium acetate pH 5.6 (3 M) 395.2 μl of water (100 v/v)
D1	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)
D2	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v)
D3	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 500.0 μl of water (100 v/v)
D4	2-methyl-2,4-pentanediol BCC 70 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v)
D5	2-methyl-2,4-pentanediol BCC 40 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 25.5 w/v - Ammonium sulfate 0.17 M - Glycerol 15 v/v - 🔍	NA	48.6 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 291.4 μl of water (100 v/v)
D7	2-Propanol 14 v/v - Sodium acetate 0.07 M pH 4.6 Calcium chloride 0.14 M - Glycerol 30 v/v - 🔍	pH 4.6	140.0 μl of 2-Propanol (100 v/v) 70.0 μl of Calcium chloride (2 M) 300.0 μl of Glycerol (100 v/v) 13.8 μl of Sodium acetate pH 3.6 (3 M) 9.5 μl of Sodium acetate pH 5.6 (3 M) 466.7 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 16 w/v - Potassium di-hydrogen phosphate 0.04 M - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 26.7 μl of Potassium di-hydrogen phosphate (1.5 M) 453.3 μl of water (100 v/v)
E1	Magnesium sulfate 1.6 M - MES 0.1 M pH 6.5 🔍	pH 6.5	48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 800.0 μl of Magnesium sulfate (2 M)
E2	Polyethylene glycol 6,000 10 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v)
E3	Polyethylene glycol 8,000 14.4 w/v - Sodium cacodylate 0.08 M pH 6.5 Calcium acetate 0.16 M - Glycerol 20 v/v - 🔍	pH 6.5	160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 24.9 μl of Sodium cacodylate pH 5 (1 M) 55.1 μl of Sodium cacodylate pH 7.4 (1 M) 272.0 μl of water (100 v/v)
E4	Jeffamine® M-600 30 v/v - MES 0.1 M pH 6.5 Cesium chloride 0.05 M - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 300.0 μl of Jeffamine® M-600 (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 550.0 μl of water (100 v/v)
E5	Ammonium sulfate 3.2 M - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v)
E6	Polyethylene glycol 10,000 15 w/v - tri-Sodium citrate 0.1 M pH 5.5 1,4-Dioxane 2 v/v - 🔍	pH 5.5	20.0 μl of 1,4-Dioxane (100 v/v) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 72.6 μl of tri-Sodium citrate pH 3 (0.5 M) 127.4 μl of tri-Sodium citrate pH 6 (0.5 M) 480.0 μl of water (100 v/v)
E7	Jeffamine® M-600 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Jeffamine® M-600 (100 v/v) 700.0 μl of water (100 v/v)
E8	2-methyl-2,4-pentanediol BCC 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of water (100 v/v)
F1	Polyethylene glycol 400 28 v/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v)
F2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 30 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 342.9 μl of water (100 v/v)
F4	Polyethylene glycol monomethyl ether 5,000 30 w/v - tris chloride BCC 0.1 M pH 8 Lithium sulfate 0.2 M - 🔍	pH 8	80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 220.0 μl of water (100 v/v)
F5	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Glycerol 12 v/v - 🔍	pH 8.5	428.6 μl of Ammonium sulfate (3.5 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 351.4 μl of water (100 v/v)
F6	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium chloride 200 mM - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400.0 μl of Ammonium chloride (500 mM) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
F7	Polyethylene glycol monomethyl ether 5,000 30 w/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.2 M - 🔍	pH 6.5	57.1 μl of Ammonium sulfate (3.5 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 142.9 μl of water (100 v/v)
F8	Polyethylene glycol 10,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v)