C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard III

Rigaku / Wizard III

Created 2009-11-16.

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Wizard III

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Wizard III

Screen Structure

Wizard III

Well Count	48
Number of distinct ingredients	45
Internal diversity (score mean dist)	0.954

Screen Composition

More information about screen composition chart.

ingredient type	Wizard III
Buffer	45
Cryoprotectant	6
Organic (non-volatile)	17
Organic (volatile)	5
Polymer	34
Salt	42

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard III Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,350 20 w/v - di-Ammonium hydrogen citrate 0.2 M - 🔍	NA	80 μl of di-Ammonium hydrogen citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.02 M - 🔍	pH 4.6	10 μl of Calcium chloride (2 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 19 μl of Sodium acetate pH 3.6 (3 M) 13 μl of Sodium acetate pH 5.6 (3 M) 656 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - Magnesium formate 0.2 M - 🔍	NA	200 μl of Magnesium formate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v)
A4	Polyethylene glycol 3,350 20 w/v - Ammonium formate 0.2 M - 🔍	NA	20 μl of Ammonium formate (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v)
A5	Polyethylene glycol 3,350 20 w/v - Ammonium chloride 0.2 M - 🔍	NA	40 μl of Ammonium chloride (5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 560 μl of water (100 v/v)
A6	Polyethylene glycol 3,350 20 w/v - Potassium formate 0.2 M - 🔍	NA	14 μl of Potassium formate (14 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 585 μl of water (100 v/v)
A7	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 0.2 M - 🔍	pH 8.5	500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Ammonium di-hydrogen phosphate (2.5 M) 320 μl of water (100 v/v)
A8	Polyethylene glycol 3,350 20 w/v - Potassium nitrate 0.2 M - 🔍	NA	66 μl of Potassium nitrate (3 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 533 μl of water (100 v/v)
B1	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	228 μl of Ammonium sulfate (3.5 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 671 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 20 w/v - Sodium thiocyanate 0.2 M - 🔍	NA	25 μl of Sodium thiocyanate (8 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 575 μl of water (100 v/v)
B3	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 500 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 10 w/v - HEPES 0.1 M pH 7.5 Ethylene glycol 8 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 80 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 620 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 8 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	19 μl of Sodium acetate pH 3.6 (3 M) 13 μl of Sodium acetate pH 5.6 (3 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 806 μl of water (100 v/v)
B6	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 500 μl of water (100 v/v)
B7	tri-Sodium citrate 1.6 M - 🔍	NA	400 μl of tri-Sodium citrate (4 M) 600 μl of water (100 v/v)
B8	Polyethylene glycol 3,350 20 w/v - tri-Potassium citrate 0.2 M - 🔍	NA	133 μl of tri-Potassium citrate (1.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 466 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 2-Propanol 10 v/v - 🔍	pH 5.5	100 μl of 2-Propanol (100 v/v) 188 μl of tri-Sodium citrate pH 6 (0.5 M) 11 μl of tri-Sodium citrate pH 3 (0.5 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 300 μl of water (100 v/v)
C2	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 4 Lithium chloride 1 M - 🔍	pH 4	100 μl of Lithium chloride (10 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 400 μl of water (100 v/v)
C3	Polyethylene glycol 3,350 20 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20 μl of Ammonium nitrate (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v)
C4	Polyethylene glycol 6,000 10 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v)
C5	Sodium Potassium phosphate 1.6 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 533 μl of Sodium Potassium phosphate (3 M) 366 μl of water (100 v/v)
C6	Ethanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	200 μl of Ethanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 700 μl of water (100 v/v)
C7	Polyethylene glycol 20,000 10 w/v - BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 20 μl of 1,4-Dioxane (100 v/v) 546 μl of water (100 v/v)
C8	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	571 μl of Ammonium sulfate (3.5 M) 19 μl of Sodium acetate pH 3.6 (3 M) 13 μl of Sodium acetate pH 5.6 (3 M) 395 μl of water (100 v/v)
D1	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v)
D2	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	480 μl of Polyethylene glycol 1,500 (50 w/v) 200 μl of Glycerol (100 v/v) 320 μl of water (100 v/v)
D3	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Magnesium chloride (2 M) 500 μl of water (100 v/v)
D4	2-methyl-2,4-pentanediol BCC 70 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of water (100 v/v)
D5	2-methyl-2,4-pentanediol BCC 40 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8 (1 M) 500 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 25.5 w/v - Ammonium sulfate 0.17 M - Glycerol 15 v/v - 🔍	NA	48 μl of Ammonium sulfate (3.5 M) 510 μl of Polyethylene glycol 4,000 (50 w/v) 150 μl of Glycerol (100 v/v) 291 μl of water (100 v/v)
D7	2-Propanol 14 v/v - Sodium acetate 0.07 M pH 4.6 Calcium chloride 0.14 M - Glycerol 30 v/v - 🔍	pH 4.6	70 μl of Calcium chloride (2 M) 140 μl of 2-Propanol (100 v/v) 13 μl of Sodium acetate pH 3.6 (3 M) 9 μl of Sodium acetate pH 5.6 (3 M) 300 μl of Glycerol (100 v/v) 466 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 16 w/v - Potassium di-hydrogen phosphate 0.04 M - Glycerol 20 v/v - 🔍	NA	26 μl of Potassium di-hydrogen phosphate (1.5 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 453 μl of water (100 v/v)
E1	Magnesium sulfate 1.6 M - MES 0.1 M pH 6.5 🔍	pH 6.5	48 μl of MES pH 5.2 (0.5 M) 800 μl of Magnesium sulfate (2 M) 151 μl of MES pH 7.1 (0.5 M)
E2	Polyethylene glycol 6,000 10 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v)
E3	Polyethylene glycol 8,000 14.4 w/v - Sodium cacodylate 0.08 M pH 6.5 Calcium acetate 0.16 M - Glycerol 20 v/v - 🔍	pH 6.5	24 μl of Sodium cacodylate pH 5 (1 M) 288 μl of Polyethylene glycol 8,000 (50 w/v) 160 μl of Calcium acetate (1 M) 55 μl of Sodium cacodylate pH 7.4 (1 M) 200 μl of Glycerol (100 v/v) 272 μl of water (100 v/v)
E4	Jeffamine® M-600 30 v/v - MES 0.1 M pH 6.5 Cesium chloride 0.05 M - 🔍	pH 6.5	300 μl of Jeffamine® M-600 (100 v/v) 50 μl of Cesium chloride (1 M) 100 μl of MES pH 6.5 (1 M) 550 μl of water (100 v/v)
E5	Ammonium sulfate 3.2 M - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	800 μl of Ammonium sulfate (4 M) 100 μl of tri-Sodium citrate pH 5 (1 M) 100 μl of water (100 v/v)
E6	Polyethylene glycol 10,000 15 w/v - tri-Sodium citrate 0.1 M pH 5.5 1,4-Dioxane 2 v/v - 🔍	pH 5.5	20 μl of 1,4-Dioxane (100 v/v) 300 μl of Polyethylene glycol 10,000 (50 w/v) 188 μl of tri-Sodium citrate pH 6 (0.5 M) 11 μl of tri-Sodium citrate pH 3 (0.5 M) 480 μl of water (100 v/v)
E7	Jeffamine® M-600 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Jeffamine® M-600 (100 v/v) 700 μl of water (100 v/v)
E8	2-methyl-2,4-pentanediol BCC 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 800 μl of water (100 v/v)
F1	Polyethylene glycol 400 28 v/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 100 μl of Calcium chloride (2 M) 280 μl of Polyethylene glycol 400 (100 v/v) 520 μl of water (100 v/v)
F2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 220 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 30 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 342 μl of water (100 v/v)
F4	Polyethylene glycol monomethyl ether 5,000 30 w/v - tris chloride BCC 0.1 M pH 8 Lithium sulfate 0.2 M - 🔍	pH 8	80 μl of Lithium sulfate (2.5 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of tris chloride BCC pH 8 (1 M) 220 μl of water (100 v/v)
F5	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Glycerol 12 v/v - 🔍	pH 8.5	428 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 120 μl of Glycerol (100 v/v) 351 μl of water (100 v/v)
F6	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium chloride 200 mM - 🔍	pH 8.5	500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Ammonium chloride (500 mM)
F7	Polyethylene glycol monomethyl ether 5,000 30 w/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.2 M - 🔍	pH 6.5	600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 142 μl of water (100 v/v)
F8	Polyethylene glycol 10,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 500 μl of water (100 v/v)