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Supplemental Information

Screen Description

Precipitant Synergy

Rigaku / Precipitant Synergy
Created 2009-11-16.

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Precipitant Synergy

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Precipitant Synergy

    Screen Structure

    Precipitant Synergy

    Well Count 96
    Number of distinct ingredients 20
    Internal diversity (score mean dist) 0.878

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:38:27.120785 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Precipitant Synergy
    Buffer 99
    Cryoprotectant 6
    Organic (non-volatile) 69
    Organic (volatile) 27
    Polymer 48
    Salt 123

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Precipitant Synergy Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 2 v/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    308 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 400 1.34 v/v -
    • Ammonium sulfate 1.34 M -
    		pH 5.5
    382 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    503 μl of water (100 v/v)
    A3
    • Ammonium sulfate 0.66 M -
    • Polyethylene glycol 400 0.66 v/v -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    188 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    704 μl of water (100 v/v)
    A4
    • Ammonium sulfate 2 M -
    • Glycerol 10 v/v -
    • Magnesium sulfate 0.05 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Glycerol (100 v/v)
    25 μl of Magnesium sulfate (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    203 μl of water (100 v/v)
    A5
    • Ammonium sulfate 1.34 M -
    • Glycerol 6.7 v/v -
    • Magnesium sulfate 0.05 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    382 μl of Ammonium sulfate (3.5 M)
    67 μl of Glycerol (100 v/v)
    25 μl of Magnesium sulfate (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    425 μl of water (100 v/v)
    A6
    • Ammonium sulfate 0.66 M -
    • Glycerol 3.3 v/v -
    • Magnesium sulfate 0.05 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    188 μl of Ammonium sulfate (3.5 M)
    33 μl of Glycerol (100 v/v)
    25 μl of Magnesium sulfate (2 M)
    100 μl of Imidazole pH 6.5 (1 M)
    653 μl of water (100 v/v)
    A7
    • Ammonium sulfate 2 M -
    • 2-methyl-2,4-pentanediol BCC 1 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    571 μl of Ammonium sulfate (3.5 M)
    10 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    318 μl of water (100 v/v)
    A8
    • Ammonium sulfate 1.34 M -
    • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    382 μl of Ammonium sulfate (3.5 M)
    6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    510 μl of water (100 v/v)
    A9
    • Ammonium sulfate 0.66 M -
    • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    188 μl of Ammonium sulfate (3.5 M)
    3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    708 μl of water (100 v/v)
    A10
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 5 v/v -
    • Magnesium sulfate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    571 μl of Ammonium sulfate (3.5 M)
    25 μl of Magnesium sulfate (2 M)
    50 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    253 μl of water (100 v/v)
    A11
    • Ammonium sulfate 1.34 M -
    • Polyethylene glycol 400 3.35 v/v -
    • Magnesium sulfate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    382 μl of Ammonium sulfate (3.5 M)
    25 μl of Magnesium sulfate (2 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    458 μl of water (100 v/v)
    A12
    • Ammonium sulfate 0.66 M -
    • Polyethylene glycol 400 1.65 v/v -
    • Magnesium sulfate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    188 μl of Ammonium sulfate (3.5 M)
    25 μl of Magnesium sulfate (2 M)
    16 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    669 μl of water (100 v/v)
    B1
    • Sodium chloride 3.9 M -
    • Polyethylene glycol 400 2 v/v -
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    780 μl of Sodium chloride (5 M)
    25 μl of Magnesium chloride (4 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    75 μl of water (100 v/v)
    B2
    • Sodium chloride 2.61 M -
    • Polyethylene glycol 400 1.34 v/v -
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    522 μl of Sodium chloride (5 M)
    25 μl of Magnesium chloride (4 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    339 μl of water (100 v/v)
    B3
    • Sodium chloride 1.29 M -
    • Polyethylene glycol 400 0.66 v/v -
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    257 μl of Sodium chloride (5 M)
    25 μl of Magnesium chloride (4 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    611 μl of water (100 v/v)
    B4
    • Sodium chloride 3 M -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    600 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    200 μl of water (100 v/v)
    B5
    • Sodium chloride 2.01 M -
    • 2-methyl-2,4-pentanediol BCC 3.35 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    402 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    33 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    414 μl of water (100 v/v)
    B6
    • Sodium chloride 0.99 M -
    • 2-methyl-2,4-pentanediol BCC 1.65 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    198 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 6.5 (1 M)
    16 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    635 μl of water (100 v/v)
    B7
    • Sodium chloride 4 M -
    • 2-Propanol 5 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    800 μl of Sodium chloride (5 M)
    50 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of water (100 v/v)
    B8
    • Sodium chloride 2.68 M -
    • 2-Propanol 3.35 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    536 μl of Sodium chloride (5 M)
    33 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    330 μl of water (100 v/v)
    B9
    • Sodium chloride 1.32 M -
    • 2-Propanol 1.65 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    264 μl of Sodium chloride (5 M)
    16 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    619 μl of water (100 v/v)
    B10
    • 2-Propanol 5 v/v -
    • Potassium Sodium phosphate 2.5 M pH 5.5
    		pH 5.5
    50 μl of 2-Propanol (100 v/v)
    833 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    116 μl of water (100 v/v)
    B11
    • 2-Propanol 3.35 v/v -
    • Potassium Sodium phosphate 1.67 M pH 5.5
    		pH 5.5
    33 μl of 2-Propanol (100 v/v)
    556 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    409 μl of water (100 v/v)
    B12
    • 2-Propanol 1.65 v/v -
    • Potassium Sodium phosphate 0.825 M pH 5.5
    		pH 5.5
    16 μl of 2-Propanol (100 v/v)
    275 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    708 μl of water (100 v/v)
    C1
    • Polyethylene glycol 400 2 v/v -
    • Potassium Sodium phosphate 2 M pH 6.5
    		pH 6.5
    666 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    313 μl of water (100 v/v)
    C2
    • Polyethylene glycol 400 1.34 v/v -
    • Potassium Sodium phosphate 1.34 M pH 6.5
    		pH 6.5
    446 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    13 μl of Polyethylene glycol 400 (100 v/v)
    539 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 0.66 v/v -
    • Potassium Sodium phosphate 0.66 M pH 6.5
    		pH 6.5
    220 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    6 μl of Polyethylene glycol 400 (100 v/v)
    773 μl of water (100 v/v)
    C4
    • Glycerol 20 v/v -
    • Potassium Sodium phosphate 2.5 M pH 7.5
    		pH 7.5
    200 μl of Glycerol (100 v/v)
    625 μl of Potassium Sodium phosphate pH 7.5 (4 M)
    175 μl of water (100 v/v)
    C5
    • Glycerol 13.4 v/v -
    • Potassium Sodium phosphate 1.68 M pH 7.5
    		pH 7.5
    134 μl of Glycerol (100 v/v)
    418 μl of Potassium Sodium phosphate pH 7.5 (4 M)
    447 μl of water (100 v/v)
    C6
    • Glycerol 6.6 v/v -
    • Potassium Sodium phosphate 0.825 M pH 7.5
    		pH 7.5
    66 μl of Glycerol (100 v/v)
    206 μl of Potassium Sodium phosphate pH 7.5 (4 M)
    727 μl of water (100 v/v)
    C7
    • 2-methyl-2,4-pentanediol BCC 8 v/v -
    • Potassium Sodium phosphate 1 M pH 8.5
    		pH 8.5
    500 μl of Potassium Sodium phosphate pH 8.5 (2 M)
    80 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    420 μl of water (100 v/v)
    C8
    • 2-methyl-2,4-pentanediol BCC 5.36 v/v -
    • Potassium Sodium phosphate 0.67 M pH 8.5
    		pH 8.5
    335 μl of Potassium Sodium phosphate pH 8.5 (2 M)
    53 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    611 μl of water (100 v/v)
    C9
    • 2-methyl-2,4-pentanediol BCC 2.64 v/v -
    • Potassium Sodium phosphate 0.33 M pH 8.5
    		pH 8.5
    165 μl of Potassium Sodium phosphate pH 8.5 (2 M)
    26 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    808 μl of water (100 v/v)
    C10
    • 2-methyl-2,4-pentanediol BCC 1 v/v -
    • tri-Ammonium citrate 2 M pH 4.5
    		pH 4.5
    10 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    666 μl of tri-Ammonium citrate pH 4.5 (3 M)
    323 μl of water (100 v/v)
    C11
    • 2-methyl-2,4-pentanediol BCC 0.67 v/v -
    • tri-Ammonium citrate 1.34 M pH 4.5
    		pH 4.5
    6 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    446 μl of tri-Ammonium citrate pH 4.5 (3 M)
    546 μl of water (100 v/v)
    C12
    • 2-methyl-2,4-pentanediol BCC 0.33 v/v -
    • tri-Ammonium citrate 0.66 M pH 4.5
    		pH 4.5
    3 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    220 μl of tri-Ammonium citrate pH 4.5 (3 M)
    776 μl of water (100 v/v)
    D1
    • 2-Propanol 5 v/v -
    • tri-Ammonium citrate 2 M pH 6.5
    		pH 6.5
    50 μl of 2-Propanol (100 v/v)
    6 μl of tri-Ammonium citrate pH 4.5 (3 M)
    660 μl of tri-Ammonium citrate pH 7.5 (3 M)
    283 μl of water (100 v/v)
    D2
    • 2-Propanol 3.35 v/v -
    • tri-Ammonium citrate 1.34 M pH 6.5
    		pH 6.5
    33 μl of 2-Propanol (100 v/v)
    4 μl of tri-Ammonium citrate pH 4.5 (3 M)
    442 μl of tri-Ammonium citrate pH 7.5 (3 M)
    519 μl of water (100 v/v)
    D3
    • 2-Propanol 1.65 v/v -
    • tri-Ammonium citrate 0.66 M pH 6.5
    		pH 6.5
    16 μl of 2-Propanol (100 v/v)
    2 μl of tri-Ammonium citrate pH 4.5 (3 M)
    217 μl of tri-Ammonium citrate pH 7.5 (3 M)
    763 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 5 v/v -
    • tri-Ammonium citrate 2 M pH 7.5
    		pH 7.5
    50 μl of Polyethylene glycol 400 (100 v/v)
    666 μl of tri-Ammonium citrate pH 7.5 (3 M)
    283 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 3.35 v/v -
    • tri-Ammonium citrate 1.34 M pH 7.5
    		pH 7.5
    33 μl of Polyethylene glycol 400 (100 v/v)
    446 μl of tri-Ammonium citrate pH 7.5 (3 M)
    519 μl of water (100 v/v)
    D6
    • Polyethylene glycol 400 1.65 v/v -
    • tri-Ammonium citrate 0.66 M pH 7.5
    		pH 7.5
    16 μl of Polyethylene glycol 400 (100 v/v)
    220 μl of tri-Ammonium citrate pH 7.5 (3 M)
    763 μl of water (100 v/v)
    D7
    • Lithium sulfate 2 M -
    • 2-Propanol 5 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    666 μl of Lithium sulfate (3 M)
    133 μl of water (100 v/v)
    D8
    • Lithium sulfate 1.34 M -
    • 2-Propanol 3.35 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    33 μl of 2-Propanol (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    446 μl of Lithium sulfate (3 M)
    369 μl of water (100 v/v)
    D9
    • Lithium sulfate 0.66 M -
    • 2-Propanol 1.65 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    16 μl of 2-Propanol (100 v/v)
    50 μl of Magnesium sulfate (2 M)
    220 μl of Lithium sulfate (3 M)
    613 μl of water (100 v/v)
    D10
    • Lithium sulfate 2 M -
    • Polyethylene glycol 400 5 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium sulfate (2 M)
    50 μl of Polyethylene glycol 400 (100 v/v)
    666 μl of Lithium sulfate (3 M)
    133 μl of water (100 v/v)
    D11
    • Lithium sulfate 1.34 M -
    • Polyethylene glycol 400 3.35 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium sulfate (2 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    446 μl of Lithium sulfate (3 M)
    369 μl of water (100 v/v)
    D12
    • Lithium sulfate 0.66 M -
    • Polyethylene glycol 400 1.65 v/v -
    • Magnesium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium sulfate (2 M)
    16 μl of Polyethylene glycol 400 (100 v/v)
    220 μl of Lithium sulfate (3 M)
    613 μl of water (100 v/v)
    E1
    • Lithium sulfate 1.7 M -
    • 2-methyl-2,4-pentanediol BCC 6.8 v/v -
    • Imidazole 0.085 M pH 6.5
    		pH 6.5
    85 μl of Imidazole pH 6.5 (1 M)
    68 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    566 μl of Lithium sulfate (3 M)
    280 μl of water (100 v/v)
    E2
    • Lithium sulfate 1.14 M -
    • 2-methyl-2,4-pentanediol BCC 4.56 v/v -
    • Imidazole 0.085 M pH 6.5
    		pH 6.5
    85 μl of Imidazole pH 6.5 (1 M)
    45 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    379 μl of Lithium sulfate (3 M)
    489 μl of water (100 v/v)
    E3
    • Lithium sulfate 0.56 M -
    • 2-methyl-2,4-pentanediol BCC 2.24 v/v -
    • Imidazole 0.085 M pH 6.5
    		pH 6.5
    85 μl of Imidazole pH 6.5 (1 M)
    22 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    186 μl of Lithium sulfate (3 M)
    705 μl of water (100 v/v)
    E4
    • Lithium sulfate 2 M -
    • Polyethylene glycol 400 2 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    666 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    213 μl of water (100 v/v)
    E5
    • Lithium sulfate 1.34 M -
    • Polyethylene glycol 400 1.34 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    13 μl of Polyethylene glycol 400 (100 v/v)
    446 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    439 μl of water (100 v/v)
    E6
    • Lithium sulfate 0.66 M -
    • Polyethylene glycol 400 0.66 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    6 μl of Polyethylene glycol 400 (100 v/v)
    220 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    673 μl of water (100 v/v)
    E7
    • Lithium sulfate 0.95 M -
    • 2-methyl-2,4-pentanediol BCC 14.2 v/v -
    • Magnesium sulfate 0.095 M -
    • Sodium acetate 0.095 M pH 4.5
    		pH 4.5
    95 μl of Sodium acetate pH 4.5 (1 M)
    47 μl of Magnesium sulfate (2 M)
    142 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    316 μl of Lithium sulfate (3 M)
    398 μl of water (100 v/v)
    E8
    • Lithium sulfate 0.636 M -
    • 2-methyl-2,4-pentanediol BCC 9.55 v/v -
    • Magnesium sulfate 0.095 M -
    • Sodium acetate 0.095 M pH 4.5
    		pH 4.5
    95 μl of Sodium acetate pH 4.5 (1 M)
    47 μl of Magnesium sulfate (2 M)
    95 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    212 μl of Lithium sulfate (3 M)
    549 μl of water (100 v/v)
    E9
    • Lithium sulfate 0.314 M -
    • 2-methyl-2,4-pentanediol BCC 4.7 v/v -
    • Magnesium sulfate 0.095 M -
    • Sodium acetate 0.095 M pH 4.5
    		pH 4.5
    95 μl of Sodium acetate pH 4.5 (1 M)
    47 μl of Magnesium sulfate (2 M)
    47 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    104 μl of Lithium sulfate (3 M)
    705 μl of water (100 v/v)
    E10
    • 2-methyl-2,4-pentanediol BCC 22.5 v/v -
    • Sodium acetate 0.675 M pH 5.5
    		pH 5.5
    675 μl of Sodium acetate pH 5.5 (1 M)
    225 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of water (100 v/v)
    E11
    • 2-methyl-2,4-pentanediol BCC 15.1 v/v -
    • Sodium acetate 0.453 M pH 5.5
    		pH 5.5
    453 μl of Sodium acetate pH 5.5 (1 M)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    396 μl of water (100 v/v)
    E12
    • 2-methyl-2,4-pentanediol BCC 7.42 v/v -
    • Sodium acetate 0.223 M pH 5.5
    		pH 5.5
    223 μl of Sodium acetate pH 5.5 (1 M)
    74 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    702 μl of water (100 v/v)
    F1
    • Ammonium sulfate 1.5 M -
    • 2-Propanol 12 v/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    428 μl of Ammonium sulfate (3.5 M)
    120 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    351 μl of water (100 v/v)
    F2
    • Ammonium sulfate 1 M -
    • 2-Propanol 8.04 v/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    287 μl of Ammonium sulfate (3.5 M)
    80 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    532 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.495 M -
    • 2-Propanol 3.96 v/v -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    141 μl of Ammonium sulfate (3.5 M)
    39 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    719 μl of water (100 v/v)
    F4
    • Sodium chloride 1.3 M -
    • 2-Propanol 30 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    260 μl of Sodium chloride (5 M)
    300 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    50 μl of Calcium chloride (2 M)
    290 μl of water (100 v/v)
    F5
    • Sodium chloride 0.871 M -
    • 2-Propanol 20.1 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    174 μl of Sodium chloride (5 M)
    201 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    50 μl of Calcium chloride (2 M)
    474 μl of water (100 v/v)
    F6
    • Sodium chloride 0.429 M -
    • 2-Propanol 9.9 v/v -
    • Imidazole 0.1 M pH 6.5
    • Calcium chloride 0.1 M -
    		pH 6.5
    85 μl of Sodium chloride (5 M)
    99 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    50 μl of Calcium chloride (2 M)
    665 μl of water (100 v/v)
    F7
    • Sodium chloride 4 M -
    • Polyethylene glycol 400 10 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    800 μl of Sodium chloride (5 M)
    100 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    F8
    • Sodium chloride 2.68 M -
    • Polyethylene glycol 400 6.7 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    536 μl of Sodium chloride (5 M)
    67 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    297 μl of water (100 v/v)
    F9
    • Sodium chloride 1.32 M -
    • Polyethylene glycol 400 3.3 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    264 μl of Sodium chloride (5 M)
    33 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    603 μl of water (100 v/v)
    F10
    • Polyethylene glycol 400 20 v/v -
    • Potassium Sodium phosphate 0.5 M pH 7.5
    		pH 7.5
    166 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    633 μl of water (100 v/v)
    F11
    • Polyethylene glycol 400 13.4 v/v -
    • Potassium Sodium phosphate 0.335 M pH 7.5
    		pH 7.5
    111 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    134 μl of Polyethylene glycol 400 (100 v/v)
    754 μl of water (100 v/v)
    F12
    • Polyethylene glycol 400 6.6 v/v -
    • Potassium Sodium phosphate 0.165 M pH 7.5
    		pH 7.5
    55 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    66 μl of Polyethylene glycol 400 (100 v/v)
    879 μl of water (100 v/v)
    G1
    • 2-Propanol 15 v/v -
    • tri-Ammonium citrate 1 M pH 8.5
    		pH 8.5
    150 μl of 2-Propanol (100 v/v)
    500 μl of tri-Ammonium citrate pH 8.5 (2 M)
    350 μl of water (100 v/v)
    G2
    • 2-Propanol 10.1 v/v -
    • tri-Ammonium citrate 0.67 M pH 8.5
    		pH 8.5
    100 μl of 2-Propanol (100 v/v)
    335 μl of tri-Ammonium citrate pH 8.5 (2 M)
    564 μl of water (100 v/v)
    G3
    • 2-Propanol 4.95 v/v -
    • tri-Ammonium citrate 0.33 M pH 8.5
    		pH 8.5
    49 μl of 2-Propanol (100 v/v)
    165 μl of tri-Ammonium citrate pH 8.5 (2 M)
    785 μl of water (100 v/v)
    G4
    • Sodium formate 2 M -
    • 2-Propanol 15 v/v -
    • Polyethylene glycol 3,350 2.5 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    285 μl of Sodium formate (7 M)
    50 μl of Polyethylene glycol 3,350 (50 w/v)
    150 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    414 μl of water (100 v/v)
    G5
    • Sodium formate 1.34 M -
    • 2-Propanol 10.1 v/v -
    • Polyethylene glycol 3,350 1.68 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    191 μl of Sodium formate (7 M)
    33 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    574 μl of water (100 v/v)
    G6
    • Sodium formate 0.66 M -
    • 2-Propanol 4.95 v/v -
    • Polyethylene glycol 3,350 0.825 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    94 μl of Sodium formate (7 M)
    16 μl of Polyethylene glycol 3,350 (50 w/v)
    49 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    739 μl of water (100 v/v)
    G7
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Polyethylene glycol 1,500 25 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of water (100 v/v)
    G8
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 1,500 16.8 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    335 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    364 μl of water (100 v/v)
    G9
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 1,500 8.25 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    165 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    636 μl of water (100 v/v)
    G10
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Polyethylene glycol 8,000 15 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    250 μl of water (100 v/v)
    G11
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 8,000 10.1 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    201 μl of Polyethylene glycol 8,000 (50 w/v)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    448 μl of water (100 v/v)
    G12
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 8,000 4.95 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 5.5
    		pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    99 μl of Polyethylene glycol 8,000 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Calcium chloride (2 M)
    652 μl of water (100 v/v)
    H1
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Polyethylene glycol 3,350 10 w/v -
    • Ammonium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    342 μl of water (100 v/v)
    H2
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 3,350 6.7 w/v -
    • Ammonium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    134 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    507 μl of water (100 v/v)
    H3
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 3,350 3.3 w/v -
    • Ammonium sulfate 0.2 M -
    • Imidazole 0.1 M pH 6.5
    		pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    66 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    677 μl of water (100 v/v)
    H4
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Polyethylene glycol 1,500 4 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    80 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    H5
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 1,500 2.68 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    53 μl of Polyethylene glycol 1,500 (50 w/v)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    645 μl of water (100 v/v)
    H6
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 1,500 1.32 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    26 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    774 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Polyethylene glycol 8,000 8 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of Sodium chloride (5 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    340 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 20.1 v/v -
    • Polyethylene glycol 8,000 5.36 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of Sodium chloride (5 M)
    107 μl of Polyethylene glycol 8,000 (50 w/v)
    201 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    491 μl of water (100 v/v)
    H9
    • 2-methyl-2,4-pentanediol BCC 9.9 v/v -
    • Polyethylene glycol 8,000 2.64 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of Sodium chloride (5 M)
    52 μl of Polyethylene glycol 8,000 (50 w/v)
    99 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    648 μl of water (100 v/v)
    H10
    • 2-Propanol 30 v/v -
    • Polyethylene glycol 3,350 4 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    470 μl of water (100 v/v)
    H11
    • 2-Propanol 20.1 v/v -
    • Polyethylene glycol 3,350 2.68 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    53 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    201 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    595 μl of water (100 v/v)
    H12
    • 2-Propanol 9.9 v/v -
    • Polyethylene glycol 3,350 1.32 w/v -
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    26 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    99 μl of 2-Propanol (100 v/v)
    50 μl of Calcium chloride (2 M)
    724 μl of water (100 v/v)