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Supplemental Information

Screen Description

Clear Strategy I

Molecular Dimensions / Clear Strategy I
Created 2009-11-16.

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Clear Strategy I

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Screen Structure

Clear Strategy I

Well Count 96
Number of distinct ingredients 15
Internal diversity (score mean dist) 0.829

Screen Composition

2025-08-16T14:27:58.962112 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Clear Strategy I
Buffer 128
Polymer 144
Salt 144

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Clear Strategy I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.3 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium acetate (3 M)
300.0 μl of water (100 v/v)
A2
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
300.0 μl of water (100 v/v)
A3
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
300.0 μl of water (100 v/v)
A4
  • Potassium bromide 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
350.0 μl of water (100 v/v)
A5
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
375.0 μl of water (100 v/v)
A6
  • Sodium formate 0.8 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
114.3 μl of Sodium formate (7 M)
285.7 μl of water (100 v/v)
A7
  • Sodium acetate 0.3 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium acetate (3 M)
500.0 μl of water (100 v/v)
A8
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A9
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A10
  • Potassium bromide 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
550.0 μl of water (100 v/v)
A11
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
575.0 μl of water (100 v/v)
A12
  • Sodium formate 0.8 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
114.3 μl of Sodium formate (7 M)
485.7 μl of water (100 v/v)
B1
  • Sodium acetate 0.3 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium acetate (3 M)
400.0 μl of water (100 v/v)
B2
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
B3
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
B4
  • Potassium bromide 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
450.0 μl of water (100 v/v)
B5
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
475.0 μl of water (100 v/v)
B6
  • Sodium formate 0.8 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
114.3 μl of Sodium formate (7 M)
385.7 μl of water (100 v/v)
B7
  • Sodium acetate 0.3 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
100.0 μl of Sodium acetate (3 M)
453.3 μl of water (100 v/v)
B8
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
453.3 μl of water (100 v/v)
B9
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
453.3 μl of water (100 v/v)
B10
  • Potassium bromide 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
503.3 μl of water (100 v/v)
B11
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
528.3 μl of water (100 v/v)
B12
  • Sodium formate 0.8 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
114.3 μl of Sodium formate (7 M)
439.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
C2
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
C3
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
C4
  • Potassium bromide 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
350.0 μl of water (100 v/v)
C5
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
375.0 μl of water (100 v/v)
C6
  • Sodium formate 0.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
114.3 μl of Sodium formate (7 M)
285.7 μl of water (100 v/v)
C7
  • Sodium acetate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
C8
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
C9
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
C10
  • Potassium bromide 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
C11
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
575.0 μl of water (100 v/v)
C12
  • Sodium formate 0.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
114.3 μl of Sodium formate (7 M)
485.7 μl of water (100 v/v)
D1
  • Sodium acetate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
D2
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
D3
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
D4
  • Potassium bromide 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
450.0 μl of water (100 v/v)
D5
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
475.0 μl of water (100 v/v)
D6
  • Sodium formate 0.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
114.3 μl of Sodium formate (7 M)
385.7 μl of water (100 v/v)
D7
  • Sodium acetate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
453.3 μl of water (100 v/v)
D8
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
453.3 μl of water (100 v/v)
D9
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
453.3 μl of water (100 v/v)
D10
  • Potassium bromide 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
503.3 μl of water (100 v/v)
D11
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
528.3 μl of water (100 v/v)
D12
  • Sodium formate 0.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
114.3 μl of Sodium formate (7 M)
439.0 μl of water (100 v/v)
E1
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
E2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
E3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
E4
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
350.0 μl of water (100 v/v)
E5
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
375.0 μl of water (100 v/v)
E6
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
285.7 μl of water (100 v/v)
E7
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
E8
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
E9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
E10
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
550.0 μl of water (100 v/v)
E11
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
575.0 μl of water (100 v/v)
E12
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
485.7 μl of water (100 v/v)
F1
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
F2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
100.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
F3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
F4
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
450.0 μl of water (100 v/v)
F5
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
475.0 μl of water (100 v/v)
F6
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
385.7 μl of water (100 v/v)
F7
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
453.3 μl of water (100 v/v)
F8
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
100.0 μl of Lithium sulfate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
453.3 μl of water (100 v/v)
F9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
100.0 μl of Magnesium chloride (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
453.3 μl of water (100 v/v)
F10
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
503.3 μl of water (100 v/v)
F11
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
528.3 μl of water (100 v/v)
F12
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
439.0 μl of water (100 v/v)
G1
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G4
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
G5
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
375.0 μl of water (100 v/v)
G6
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
285.7 μl of water (100 v/v)
G7
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G8
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G10
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
G11
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
575.0 μl of water (100 v/v)
G12
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
485.7 μl of water (100 v/v)
H1
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H4
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
450.0 μl of water (100 v/v)
H5
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
H6
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
385.7 μl of water (100 v/v)
H7
  • Sodium acetate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
453.3 μl of water (100 v/v)
H8
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
453.3 μl of water (100 v/v)
H9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
453.3 μl of water (100 v/v)
H10
  • Potassium bromide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
503.3 μl of water (100 v/v)
H11
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
528.3 μl of water (100 v/v)
H12
  • Sodium formate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
114.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
439.0 μl of water (100 v/v)