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Supplemental Information

Screen Description

Clear Strategy I

Molecular Dimensions / Clear Strategy I
Created 2009-11-16.

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Clear Strategy I

    Screen Structure

    Clear Strategy I

    Well Count 96
    Number of distinct ingredients 15
    Internal diversity (score mean dist) 0.829

    Screen Composition

    2025-06-13T21:36:50.756841 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Clear Strategy I
    Buffer 128
    Polymer 144
    Salt 144

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Clear Strategy I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.3 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    100 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    300 μl of water (100 v/v)
    A2
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    300 μl of water (100 v/v)
    A3
    • Magnesium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    300 μl of water (100 v/v)
    A4
    • Potassium bromide 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    50 μl of Potassium bromide (4 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    350 μl of water (100 v/v)
    A5
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    375 μl of water (100 v/v)
    A6
    • Sodium formate 0.8 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5.5
    114 μl of Sodium formate (7 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    285 μl of water (100 v/v)
    A7
    • Sodium acetate 0.3 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    100 μl of Sodium acetate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    A8
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    A9
    • Magnesium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    A10
    • Potassium bromide 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    50 μl of Potassium bromide (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    550 μl of water (100 v/v)
    A11
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    575 μl of water (100 v/v)
    A12
    • Sodium formate 0.8 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    114 μl of Sodium formate (7 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    485 μl of water (100 v/v)
    B1
    • Sodium acetate 0.3 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    100 μl of Sodium acetate (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    400 μl of water (100 v/v)
    B2
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    400 μl of water (100 v/v)
    B3
    • Magnesium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    400 μl of water (100 v/v)
    B4
    • Potassium bromide 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    50 μl of Potassium bromide (4 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    450 μl of water (100 v/v)
    B5
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    475 μl of water (100 v/v)
    B6
    • Sodium formate 0.8 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 5.5
    114 μl of Sodium formate (7 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    385 μl of water (100 v/v)
    B7
    • Sodium acetate 0.3 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    100 μl of Sodium acetate (3 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    453 μl of water (100 v/v)
    B8
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Lithium sulfate (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    453 μl of water (100 v/v)
    B9
    • Magnesium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Magnesium chloride (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    453 μl of water (100 v/v)
    B10
    • Potassium bromide 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of Potassium bromide (4 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    503 μl of water (100 v/v)
    B11
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    528 μl of water (100 v/v)
    B12
    • Sodium formate 0.8 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    114 μl of Sodium formate (7 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5.5 (1 M)
    439 μl of water (100 v/v)
    C1
    • Sodium acetate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    100 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of water (100 v/v)
    C2
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of water (100 v/v)
    C3
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of water (100 v/v)
    C4
    • Potassium bromide 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    350 μl of water (100 v/v)
    C5
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    375 μl of water (100 v/v)
    C6
    • Sodium formate 0.8 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 6.5
    114 μl of Sodium formate (7 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    285 μl of water (100 v/v)
    C7
    • Sodium acetate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    100 μl of Sodium acetate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of water (100 v/v)
    C8
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of water (100 v/v)
    C9
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of water (100 v/v)
    C10
    • Potassium bromide 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    550 μl of water (100 v/v)
    C11
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    575 μl of water (100 v/v)
    C12
    • Sodium formate 0.8 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    114 μl of Sodium formate (7 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    485 μl of water (100 v/v)
    D1
    • Sodium acetate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    100 μl of Sodium acetate (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    D2
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    D3
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    D4
    • Potassium bromide 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    450 μl of water (100 v/v)
    D5
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    475 μl of water (100 v/v)
    D6
    • Sodium formate 0.8 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.5
    114 μl of Sodium formate (7 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    385 μl of water (100 v/v)
    D7
    • Sodium acetate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    100 μl of Sodium acetate (3 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    453 μl of water (100 v/v)
    D8
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Lithium sulfate (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    453 μl of water (100 v/v)
    D9
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Magnesium chloride (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    453 μl of water (100 v/v)
    D10
    • Potassium bromide 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    503 μl of water (100 v/v)
    D11
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    528 μl of water (100 v/v)
    D12
    • Sodium formate 0.8 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    114 μl of Sodium formate (7 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    439 μl of water (100 v/v)
    E1
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    100 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of water (100 v/v)
    E2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of water (100 v/v)
    E3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of water (100 v/v)
    E4
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    50 μl of Potassium bromide (4 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    350 μl of water (100 v/v)
    E5
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    375 μl of water (100 v/v)
    E6
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    114 μl of Sodium formate (7 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    285 μl of water (100 v/v)
    E7
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    100 μl of Sodium acetate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    500 μl of water (100 v/v)
    E8
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    500 μl of water (100 v/v)
    E9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    500 μl of water (100 v/v)
    E10
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    50 μl of Potassium bromide (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    550 μl of water (100 v/v)
    E11
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    575 μl of water (100 v/v)
    E12
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    114 μl of Sodium formate (7 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    485 μl of water (100 v/v)
    F1
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    100 μl of Sodium acetate (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of water (100 v/v)
    F2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of water (100 v/v)
    F3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of water (100 v/v)
    F4
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    50 μl of Potassium bromide (4 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    450 μl of water (100 v/v)
    F5
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    475 μl of water (100 v/v)
    F6
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7.5
    114 μl of Sodium formate (7 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    385 μl of water (100 v/v)
    F7
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    100 μl of Sodium acetate (3 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    453 μl of water (100 v/v)
    F8
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Lithium sulfate (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    453 μl of water (100 v/v)
    F9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Magnesium chloride (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    453 μl of water (100 v/v)
    F10
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of Potassium bromide (4 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    503 μl of water (100 v/v)
    F11
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    528 μl of water (100 v/v)
    F12
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    114 μl of Sodium formate (7 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    439 μl of water (100 v/v)
    G1
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    100 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G4
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    50 μl of Potassium bromide (4 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of water (100 v/v)
    G5
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    375 μl of water (100 v/v)
    G6
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 8.5
    114 μl of Sodium formate (7 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    285 μl of water (100 v/v)
    G7
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    100 μl of Sodium acetate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G8
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G10
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    50 μl of Potassium bromide (4 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    550 μl of water (100 v/v)
    G11
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    575 μl of water (100 v/v)
    G12
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 8.5
    114 μl of Sodium formate (7 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    485 μl of water (100 v/v)
    H1
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    100 μl of Sodium acetate (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H4
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    50 μl of Potassium bromide (4 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    450 μl of water (100 v/v)
    H5
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    475 μl of water (100 v/v)
    H6
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 8.5
    114 μl of Sodium formate (7 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    385 μl of water (100 v/v)
    H7
    • Sodium acetate 0.3 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    100 μl of Sodium acetate (3 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    453 μl of water (100 v/v)
    H8
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Lithium sulfate (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    453 μl of water (100 v/v)
    H9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Magnesium chloride (2 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    453 μl of water (100 v/v)
    H10
    • Potassium bromide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of Potassium bromide (4 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    503 μl of water (100 v/v)
    H11
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    528 μl of water (100 v/v)
    H12
    • Sodium formate 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 20,000 8 w/v -
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8.5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    114 μl of Sodium formate (7 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    439 μl of water (100 v/v)