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Supplemental Information

Screen Description

Clear Strategy II

Molecular Dimensions / Clear Strategy II
Created 2009-11-16.

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Clear Strategy II

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Screen Structure

Clear Strategy II

Well Count 96
Number of distinct ingredients 26
Internal diversity (score mean dist) 0.867

Screen Composition

2025-08-16T09:05:41.694285 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Clear Strategy II
Buffer 128
Organic (non-volatile) 12
Organic (volatile) 8
Polymer 60
Salt 128

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Clear Strategy II Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 1.5 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
471.4 μl of water (100 v/v)
A2
  • Lithium sulfate 0.8 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
400.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A3
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
100.0 μl of Sodium acetate pH 5.5 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
A4
  • Potassium di-hydrogen phosphate 0.5 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
250.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
650.0 μl of water (100 v/v)
A5
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5.5
100.0 μl of Calcium acetate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
300.0 μl of water (100 v/v)
A6
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 15 w/v -
pH 5.5
100.0 μl of Calcium acetate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A7
  • Ammonium sulfate 2.7 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
771.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
128.6 μl of water (100 v/v)
A8
  • Lithium sulfate 1.8 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
900.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
A9
  • Sodium formate 4 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
100.0 μl of Sodium acetate pH 5.5 (1 M)
571.4 μl of Sodium formate (7 M)
328.6 μl of water (100 v/v)
A10
  • Potassium di-hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
500.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
A11
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 5.5
100.0 μl of Calcium acetate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
A12
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 5.5
100.0 μl of Calcium acetate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
453.3 μl of water (100 v/v)
B1
  • Sodium acetate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 5.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 5.5
  • 1,4-Butanediol 40 v/v -
pH 5.5
400.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
B3
  • Cadmium chloride 0.005 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 20 w/v -
pH 5.5
5.0 μl of Cadmium chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
495.0 μl of water (100 v/v)
B4
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 5.5
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
681.2 μl of water (100 v/v)
B5
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 600 20 v/v -
pH 5.5
200.0 μl of Polyethylene glycol 600 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
681.2 μl of water (100 v/v)
B6
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 1,500 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
481.2 μl of water (100 v/v)
B7
  • Sodium acetate 0.1 M pH 5.5
  • 2-Propanol 35 v/v -
pH 5.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
550.0 μl of water (100 v/v)
B8
  • Sodium acetate 0.1 M pH 5.5
  • Jeffamine® M-600 30 v/v -
pH 5.5
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
300.0 μl of water (100 v/v)
B9
  • Nickel (II) chloride 0.005 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 20 w/v -
pH 5.5
5.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
495.0 μl of water (100 v/v)
B10
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 18 w/v -
pH 5.5
225.0 μl of Polyethylene glycol 3,350 (80 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
656.2 μl of water (100 v/v)
B11
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 5.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
521.2 μl of water (100 v/v)
B12
  • Potassium thiocyanate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 6,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
581.2 μl of water (100 v/v)
C1
  • Ammonium sulfate 1.5 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
471.4 μl of water (100 v/v)
C2
  • Lithium sulfate 0.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
400.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
C3
  • Sodium formate 2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
C4
  • Potassium di-hydrogen phosphate 0.5 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
250.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
650.0 μl of water (100 v/v)
C5
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
C6
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
C7
  • Ammonium sulfate 2.7 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
771.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
128.6 μl of water (100 v/v)
C8
  • Lithium sulfate 1.8 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
900.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
C9
  • Sodium formate 4 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
571.4 μl of Sodium formate (7 M)
328.6 μl of water (100 v/v)
C10
  • Potassium di-hydrogen phosphate 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
500.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
C11
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
C12
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 6.5
100.0 μl of Calcium acetate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
453.3 μl of water (100 v/v)
D1
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
D2
  • Sodium cacodylate 0.1 M pH 6.5
  • 1,4-Butanediol 40 v/v -
pH 6.5
400.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
D3
  • Cadmium chloride 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
5.0 μl of Cadmium chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
495.0 μl of water (100 v/v)
D4
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 6.5
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
681.2 μl of water (100 v/v)
D5
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 600 20 v/v -
pH 6.5
200.0 μl of Polyethylene glycol 600 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
681.2 μl of water (100 v/v)
D6
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 1,500 20 w/v -
pH 6.5
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
481.2 μl of water (100 v/v)
D7
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-Propanol 35 v/v -
pH 6.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
D8
  • Sodium cacodylate 0.1 M pH 6.5
  • Jeffamine® M-600 30 v/v -
pH 6.5
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
D9
  • Nickel (II) chloride 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
5.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
495.0 μl of water (100 v/v)
D10
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 3,350 18 w/v -
pH 6.5
225.0 μl of Polyethylene glycol 3,350 (80 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
656.2 μl of water (100 v/v)
D11
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
521.2 μl of water (100 v/v)
D12
  • Potassium thiocyanate 0.15 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 6,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
581.2 μl of water (100 v/v)
E1
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
471.4 μl of water (100 v/v)
E2
  • Lithium sulfate 0.8 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
E3
  • Sodium formate 2 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
285.7 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
614.3 μl of water (100 v/v)
E4
  • Potassium di-hydrogen phosphate 0.5 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
250.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
650.0 μl of water (100 v/v)
E5
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
100.0 μl of Calcium acetate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
E6
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
100.0 μl of Calcium acetate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
E7
  • Ammonium sulfate 2.7 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
771.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
128.6 μl of water (100 v/v)
E8
  • Lithium sulfate 1.8 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
900.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
E9
  • Sodium formate 4 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
328.6 μl of water (100 v/v)
E10
  • Potassium di-hydrogen phosphate 1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
E11
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7.5
100.0 μl of Calcium acetate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
400.0 μl of water (100 v/v)
E12
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 7.5
100.0 μl of Calcium acetate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
453.3 μl of water (100 v/v)
F1
  • tris chloride BCC 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 7.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
F2
  • tris chloride BCC 0.1 M pH 7.5
  • 1,4-Butanediol 40 v/v -
pH 7.5
400.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
500.0 μl of water (100 v/v)
F3
  • Cadmium chloride 0.005 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
5.0 μl of Cadmium chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
495.0 μl of water (100 v/v)
F4
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 7.5
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
681.2 μl of water (100 v/v)
F5
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 600 20 v/v -
pH 7.5
200.0 μl of Polyethylene glycol 600 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
681.2 μl of water (100 v/v)
F6
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 1,500 20 w/v -
pH 7.5
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
481.2 μl of water (100 v/v)
F7
  • tris chloride BCC 0.1 M pH 7.5
  • 2-Propanol 35 v/v -
pH 7.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
550.0 μl of water (100 v/v)
F8
  • tris chloride BCC 0.1 M pH 7.5
  • Jeffamine® M-600 30 v/v -
pH 7.5
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
F9
  • Nickel (II) chloride 0.005 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
5.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
495.0 μl of water (100 v/v)
F10
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 3,350 18 w/v -
pH 7.5
225.0 μl of Polyethylene glycol 3,350 (80 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
656.2 μl of water (100 v/v)
F11
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 7.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
521.2 μl of water (100 v/v)
F12
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 6,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
581.2 μl of water (100 v/v)
G1
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
G2
  • Lithium sulfate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G3
  • Sodium formate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
285.7 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
614.3 μl of water (100 v/v)
G4
  • Potassium di-hydrogen phosphate 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
250.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
G5
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 8.5
100.0 μl of Calcium acetate (2 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G6
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 15 w/v -
pH 8.5
100.0 μl of Calcium acetate (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G7
  • Ammonium sulfate 2.7 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
771.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
128.6 μl of water (100 v/v)
G8
  • Lithium sulfate 1.8 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
900.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
G9
  • Sodium formate 4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G10
  • Potassium di-hydrogen phosphate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G11
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 1 w/v -
pH 8.5
100.0 μl of Calcium acetate (2 M)
20.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
G12
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
pH 8.5
100.0 μl of Calcium acetate (2 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
453.3 μl of water (100 v/v)
H1
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 8.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H2
  • tris chloride BCC 0.1 M pH 8.5
  • 1,4-Butanediol 40 v/v -
pH 8.5
400.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H3
  • Cadmium chloride 0.005 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
5.0 μl of Cadmium chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
495.0 μl of water (100 v/v)
H4
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 8.5
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
681.2 μl of water (100 v/v)
H5
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 600 20 v/v -
pH 8.5
200.0 μl of Polyethylene glycol 600 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
681.2 μl of water (100 v/v)
H6
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,500 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
481.2 μl of water (100 v/v)
H7
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 35 v/v -
pH 8.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8.5
  • Jeffamine® M-600 30 v/v -
pH 8.5
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H9
  • Nickel (II) chloride 0.005 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
5.0 μl of Nickel (II) chloride (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
495.0 μl of water (100 v/v)
H10
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 18 w/v -
pH 8.5
225.0 μl of Polyethylene glycol 3,350 (80 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
656.2 μl of water (100 v/v)
H11
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 8.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
521.2 μl of water (100 v/v)
H12
  • Potassium thiocyanate 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 15 w/v -
pH 8.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
18.8 μl of Potassium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
581.2 μl of water (100 v/v)