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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG+ HTS
Barcodes
JCSG+ HTS
Screen Structure
JCSG+ HTS
Well Count | 96 |
---|---|
Number of distinct ingredients | 70 |
Internal diversity (score mean dist) | 0.961 |
Screen Composition
ingredient type | JCSG+ HTS |
---|---|
Buffer | 102 |
Cryoprotectant | 6 |
Organic (non-volatile) | 25 |
Organic (volatile) | 6 |
Polymer | 65 |
Salt | 97 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 500 μl of Polyethylene glycol 400 (100 v/v) 58 μl of Sodium acetate pH 4 (1 M) 41 μl of Sodium acetate pH 5 (1 M) 100 μl of Lithium sulfate (2 M) 300 μl of water (100 v/v) |
A2 |
| pH 5.5 | 3 μl of tri-Sodium citrate pH 3 (1.6 M) 58 μl of tri-Sodium citrate pH 6.5 (1.6 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 537 μl of water (100 v/v) |
A3 |
| NA | 80 μl of di-Ammonium hydrogen citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v) |
A4 |
| pH 4.6 | 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 10 μl of Calcium chloride (2 M) 590 μl of water (100 v/v) |
A5 |
| NA | 200 μl of Magnesium formate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
A6 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 100 μl of Lithium sulfate (2 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
A7 |
| pH 9.5 | 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of CHES pH 9.5 (1 M) 500 μl of water (100 v/v) |
A8 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium formate (10 M) 580 μl of water (100 v/v) |
A9 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium chloride (5 M) 560 μl of water (100 v/v) |
A10 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 14 μl of Potassium formate (14 M) 585 μl of water (100 v/v) |
A11 |
| pH 8.5 | 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Ammonium di-hydrogen phosphate (2.5 M) 320 μl of water (100 v/v) |
A12 |
| NA | 66 μl of Potassium nitrate (3 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 533 μl of water (100 v/v) |
B1 |
| pH 4 | 10 μl of tri-Sodium citrate pH 3 (1.6 M) 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 228 μl of Ammonium sulfate (3.5 M) 708 μl of water (100 v/v) |
B2 |
| NA | 25 μl of Sodium thiocyanate (8 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 575 μl of water (100 v/v) |
B3 |
| pH 9 | 9 μl of BICINE pH 7.4 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 90 μl of BICINE pH 9.3 (1 M) 500 μl of water (100 v/v) |
B4 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Ethylene glycol (100 v/v) 620 μl of water (100 v/v) |
B5 |
| pH 6.5 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 100 μl of Polyethylene glycol 8,000 (50 w/v) 400 μl of water (100 v/v) |
B6 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Ethanol (100 v/v) 100 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
B7 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v) |
B8 |
| pH 7 | 100 μl of Magnesium chloride (2 M) 100 μl of tris chloride BCC pH 7 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v) |
B9 |
| pH 5 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 5 μl of tri-Sodium citrate pH 3 (1.6 M) 56 μl of tri-Sodium citrate pH 6.5 (1.6 M) 537 μl of water (100 v/v) |
B10 |
| pH 6.5 | 500 μl of Polyethylene glycol 200 (100 v/v) 100 μl of Magnesium chloride (2 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of water (100 v/v) |
B11 |
| NA | 1000 μl of tri-Sodium citrate (1.6 M) |
B12 |
| NA | 80 μl of tri-Potassium citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v) |
C1 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 40 μl of Sodium chloride (5 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v) |
C2 |
| pH 4 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 10 μl of tri-Sodium citrate pH 3 (1.6 M) 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 100 μl of Lithium chloride (10 M) 437 μl of water (100 v/v) |
C3 |
| NA | 100 μl of Ammonium nitrate (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of water (100 v/v) |
C4 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
C5 |
| pH 7.5 | 160 μl of Sodium di-hydrogen phosphate (5 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of Potassium di-hydrogen phosphate (2 M) 340 μl of water (100 v/v) |
C6 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 500 μl of water (100 v/v) |
C7 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 41 μl of Sodium acetate pH 5 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 200 μl of Zinc acetate (1 M) 500 μl of water (100 v/v) |
C8 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Ethanol (100 v/v) 700 μl of water (100 v/v) |
C9 |
| pH 6.2 | 100 μl of Glycerol (100 v/v) 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 250 μl of 1,2-Propanediol (100 v/v) 616 μl of water (100 v/v) |
C10 |
| pH 9 | 9 μl of BICINE pH 7.4 (1 M) 90 μl of BICINE pH 9.3 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 20 μl of 1,4-Dioxane (100 v/v) 546 μl of water (100 v/v) |
C11 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v) |
C12 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v) |
D1 |
| NA | 200 μl of Glycerol (100 v/v) 480 μl of Polyethylene glycol 1,500 (50 w/v) 320 μl of water (100 v/v) |
D2 |
| pH 7.5 | 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
D3 |
| pH 6.2 | 500 μl of Polyethylene glycol 200 (100 v/v) 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40 μl of Sodium chloride (5 M) 426 μl of water (100 v/v) |
D4 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 41 μl of Sodium acetate pH 5 (1 M) 100 μl of Lithium sulfate (2 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of water (100 v/v) |
D5 |
| pH 7.5 | 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 0 μl of HEPES pH 5 (1 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v) |
D6 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 400 μl of water (100 v/v) |
D7 |
| pH 8.5 | 400 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Lithium sulfate (2 M) 400 μl of water (100 v/v) |
D8 |
| pH 8 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 47 μl of tris chloride BCC pH 8.5 (1 M) 53 μl of tris chloride BCC pH 7.5 (1 M) 500 μl of water (100 v/v) |
D9 |
| NA | 510 μl of Polyethylene glycol 4,000 (50 w/v) 150 μl of Glycerol (100 v/v) 48 μl of Ammonium sulfate (3.5 M) 291 μl of water (100 v/v) |
D10 |
| pH 6.5 | 400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Calcium acetate (1 M) 300 μl of water (100 v/v) |
D11 |
| pH 4.6 | 33 μl of Sodium acetate pH 4 (1 M) 36 μl of Sodium acetate pH 5 (1 M) 300 μl of Glycerol (100 v/v) 140 μl of 2-Propanol (100 v/v) 70 μl of Calcium chloride (2 M) 420 μl of water (100 v/v) |
D12 |
| NA | 200 μl of Glycerol (100 v/v) 320 μl of Polyethylene glycol 8,000 (50 w/v) 20 μl of Potassium di-hydrogen phosphate (2 M) 460 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 625 μl of tri-Sodium citrate (1.6 M) 275 μl of water (100 v/v) |
E2 |
| pH 6.5 | 40 μl of Sodium chloride (5 M) 571 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 288 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100 μl of 2-Propanol (100 v/v) 40 μl of Sodium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 760 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Lithium sulfate (2 M) 360 μl of Ammonium sulfate (3.5 M) 440 μl of water (100 v/v) |
E5 |
| pH 10 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of CAPS pH 12 (1 M) 50 μl of CAPS pH 9.5 (1 M) 500 μl of water (100 v/v) |
E6 |
| pH 8 | 400 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of Imidazole pH 8 (1 M) 200 μl of Zinc acetate (1 M) 300 μl of water (100 v/v) |
E7 |
| pH 6.5 | 100 μl of 2-Propanol (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Zinc acetate (1 M) 600 μl of water (100 v/v) |
E8 |
| pH 4.5 | 58 μl of Sodium acetate pH 4 (1 M) 41 μl of Sodium acetate pH 5 (1 M) 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 500 μl of water (100 v/v) |
E9 |
| pH 6.5 | 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 640 μl of Magnesium sulfate (2.5 M) 160 μl of water (100 v/v) |
E10 |
| pH 9 | 9 μl of BICINE pH 7.4 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 90 μl of BICINE pH 9.3 (1 M) 700 μl of water (100 v/v) |
E11 |
| pH 6.5 | 200 μl of Glycerol (100 v/v) 80 μl of Sodium cacodylate pH 6.5 (1 M) 288 μl of Polyethylene glycol 8,000 (50 w/v) 160 μl of Calcium acetate (1 M) 272 μl of water (100 v/v) |
E12 |
| pH 8 | 100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v) |
F1 |
| pH 6.5 | 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 50 μl of Cesium chloride (1 M) 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 150 μl of water (100 v/v) |
F2 |
| pH 5 | 5 μl of tri-Sodium citrate pH 3 (1.6 M) 56 μl of tri-Sodium citrate pH 6.5 (1.6 M) 914 μl of Ammonium sulfate (3.5 M) 23 μl of water (100 v/v) |
F3 |
| pH 8 | 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 47 μl of tris chloride BCC pH 8.5 (1 M) 53 μl of tris chloride BCC pH 7.5 (1 M) 700 μl of water (100 v/v) |
F4 |
| pH 7.5 | 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
F5 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 500 μl of Ethylene glycol (100 v/v) 300 μl of water (100 v/v) |
F6 |
| pH 9 | 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 9 μl of BICINE pH 7.4 (1 M) 90 μl of BICINE pH 9.3 (1 M) 800 μl of water (100 v/v) |
F7 |
| NA | 266 μl of di-Sodium succinate pH 7 (3 M) 733 μl of water (100 v/v) |
F8 |
| NA | 700 μl of di-Sodium DL-malate pH 7 (3 M) 300 μl of water (100 v/v) |
F9 |
| NA | 800 μl of di-Sodium malonate pH 7 (3 M) 200 μl of water (100 v/v) |
F10 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 10 μl of Jeffamine® ED-2001 (50 v/v) 366 μl of di-Sodium malonate pH 7 (3 M) 523 μl of water (100 v/v) |
F11 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 333 μl of di-Sodium succinate pH 7 (3 M) 20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 546 μl of water (100 v/v) |
F12 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 300 μl of water (100 v/v) |
G1 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 600 μl of Jeffamine® ED-2001 (50 v/v) 300 μl of water (100 v/v) |
G2 |
| pH 7.5 | 10 μl of Magnesium chloride (2 M) 440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v) |
G3 |
| pH 8.5 | 10 μl of Cobalt (II) chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 490 μl of water (100 v/v) |
G4 |
| pH 8.5 | 200 μl of Trimethylamine N-oxide (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 300 μl of water (100 v/v) |
G5 |
| pH 7.5 | 5 μl of Cobalt (II) chloride (1 M) 1 μl of Nickel (II) chloride (4 M) 2 μl of Magnesium chloride (2 M) 240 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 5 μl of Cadmium chloride (1 M) 646 μl of water (100 v/v) |
G6 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of di-Sodium malonate pH 7 (3 M) 533 μl of water (100 v/v) |
G7 |
| NA | 33 μl of di-Sodium succinate pH 7 (3 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 666 μl of water (100 v/v) |
G8 |
| NA | 50 μl of di-Sodium DL-malate pH 7 (3 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 550 μl of water (100 v/v) |
G9 |
| NA | 12 μl of Potassium thiocyanate (8 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 387 μl of water (100 v/v) |
G10 |
| NA | 37 μl of Potassium bromide (4 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 362 μl of water (100 v/v) |
G11 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v) |
G12 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 600 μl of Sodium chloride (5 M) 300 μl of water (100 v/v) |
H1 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of Magnesium formate (1 M) 600 μl of water (100 v/v) |
H2 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 285 μl of Ammonium sulfate (3.5 M) 20 μl of Polyethylene glycol 3,350 (50 w/v) 594 μl of water (100 v/v) |
H3 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
H4 |
| pH 5.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Calcium chloride (2 M) 350 μl of water (100 v/v) |
H5 |
| pH 5.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 250 μl of water (100 v/v) |
H6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Ammonium acetate (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 460 μl of water (100 v/v) |
H7 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 57 μl of Ammonium sulfate (3.5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 342 μl of water (100 v/v) |
H8 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 40 μl of Sodium chloride (5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 360 μl of water (100 v/v) |
H9 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Lithium sulfate (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 300 μl of water (100 v/v) |
H10 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of water (100 v/v) |
H11 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 300 μl of water (100 v/v) |
H12 |
| pH 7.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Ammonium acetate (1 M) 100 μl of HEPES pH 7.5 (1 M) 250 μl of water (100 v/v) |