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Supplemental Information

Screen Description

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 70
Internal diversity (Score mean dist)0.96
Internal diversity (Ion mean dist)0.94
Internal diversity (PEG mean dist)0.93
Internal diversity (Known pH mean dist)0.87
Internal diversity (Penalty mean dist)0.94

Screen Composition

Chemical classes bar chart
ingredient type JCSG+ HTS
Buffer 102
Cryoprotectant 6
Organic (non-volatile) 25
Organic (volatile) 6
Polymer 65
Salt 97

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG+ HTS Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Polyethylene glycol 400 50 v/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
300.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 3,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
13.12 μl of tri-Sodium citrate pH 3 (1.6 M)
49.38 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
A4
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
590.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 1,000 20 w/v -
  • Lithium sulfate 0.2 M -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.29 μl of Potassium formate (14 M)
585.71 μl of water (100 v/v)
A11
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.67 μl of Potassium nitrate (3 M)
533.33 μl of water (100 v/v)
B1
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
228.57 μl of Ammonium sulfate (3.5 M)
36.25 μl of tri-Sodium citrate pH 3 (1.6 M)
26.25 μl of tri-Sodium citrate pH 6.5 (1.6 M)
708.93 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
9.46 μl of BICINE pH 7.4 (1 M)
90.54 μl of BICINE pH 9.3 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
B5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B6
  • Ethanol 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Ethanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
740.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 8,000 10 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
600.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
20.42 μl of tri-Sodium citrate pH 3 (1.6 M)
42.08 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
B10
  • Polyethylene glycol 200 50 v/v -
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
B11
  • tri-Sodium citrate 1.6 M -
NA
1000.0 μl of tri-Sodium citrate (1.6 M)
B12
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate (2.5 M)
520.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
36.25 μl of tri-Sodium citrate pH 3 (1.6 M)
26.25 μl of tri-Sodium citrate pH 6.5 (1.6 M)
437.5 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
NA
100.0 μl of Ammonium nitrate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C5
  • Sodium di-hydrogen phosphate 0.8 M -
  • Potassium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium di-hydrogen phosphate (2 M)
160.0 μl of Sodium di-hydrogen phosphate (5 M)
340.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 300 40 v/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
C7
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 3,000 10 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
200.0 μl of Zinc acetate (1 M)
500.0 μl of water (100 v/v)
C8
  • Ethanol 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Ethanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
C9
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
33.33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
616.67 μl of water (100 v/v)
C10
  • Polyethylene glycol 20,000 10 w/v -
  • 1,4-Dioxane 2 v/v -
  • BICINE 0.1 M pH 9
pH 9
20.0 μl of 1,4-Dioxane (100 v/v)
9.46 μl of BICINE pH 7.4 (1 M)
90.54 μl of BICINE pH 9.3 (1 M)
333.33 μl of Polyethylene glycol 20,000 (30 w/v)
546.67 μl of water (100 v/v)
C11
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
571.43 μl of Ammonium sulfate (3.5 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
328.57 μl of water (100 v/v)
C12
  • Polyethylene glycol 8,000 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 30 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
33.33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
426.67 μl of water (100 v/v)
D4
  • Polyethylene glycol 8,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
200.0 μl of water (100 v/v)
D5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 400 40 v/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
52.98 μl of tris chloride BCC pH 7.5 (1 M)
47.02 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium sulfate 0.17 M -
  • Glycerol 15 v/v -
NA
48.57 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
291.43 μl of water (100 v/v)
D10
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
D11
  • Glycerol 30 v/v -
  • Calcium chloride 0.14 M -
  • 2-Propanol 14 v/v -
  • Sodium acetate 0.07 M pH 4.6
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
70.0 μl of Calcium chloride (2 M)
300.0 μl of Glycerol (100 v/v)
33.25 μl of Sodium acetate pH 4 (1 M)
36.75 μl of Sodium acetate pH 5 (1 M)
420.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 8,000 16 w/v -
  • Potassium di-hydrogen phosphate 0.04 M -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Potassium di-hydrogen phosphate (2 M)
460.0 μl of water (100 v/v)
E1
  • tri-Sodium citrate 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
E2
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
288.57 μl of water (100 v/v)
E3
  • 2-Propanol 10 v/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 1.26 M -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
440.0 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • CAPS 0.1 M pH 10.5
pH 10.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of CAPS pH 9.5 (1 M)
50.0 μl of CAPS pH 11.5 (1 M)
500.0 μl of water (100 v/v)
E6
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 3,000 20 w/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
200.0 μl of Zinc acetate (1 M)
300.0 μl of water (100 v/v)
E7
  • Zinc acetate 0.2 M -
  • 2-Propanol 10 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of Zinc acetate (1 M)
600.0 μl of water (100 v/v)
E8
  • Ammonium di-hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
58.63 μl of Sodium acetate pH 4 (1 M)
41.37 μl of Sodium acetate pH 5 (1 M)
500.0 μl of water (100 v/v)
E9
  • Magnesium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
640.0 μl of Magnesium sulfate (2.5 M)
48.62 μl of MES pH 5.2 (0.5 M)
151.38 μl of MES pH 7.1 (0.5 M)
160.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 6,000 10 w/v -
  • BICINE 0.1 M pH 9
pH 9
9.46 μl of BICINE pH 7.4 (1 M)
90.54 μl of BICINE pH 9.3 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 8,000 14.4 w/v -
  • Calcium acetate 0.16 M -
  • Glycerol 20 v/v -
  • Sodium cacodylate 0.08 M pH 6.5
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
272.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
F1
  • Jeffamine® M-600 30 v/v -
  • Cesium chloride 0.05 M -
  • MES 0.1 M pH 6.5
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
48.62 μl of MES pH 5.2 (0.5 M)
151.38 μl of MES pH 7.1 (0.5 M)
150.0 μl of water (100 v/v)
F2
  • Ammonium sulfate 3.2 M -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
914.29 μl of Ammonium sulfate (3.5 M)
20.42 μl of tri-Sodium citrate pH 3 (1.6 M)
42.08 μl of tri-Sodium citrate pH 6.5 (1.6 M)
23.21 μl of water (100 v/v)
F3
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
52.98 μl of tris chloride BCC pH 7.5 (1 M)
47.02 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
F4
  • Jeffamine® M-600 20 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
500.0 μl of water (100 v/v)
F5
  • Ethylene glycol 50 v/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F6
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
9.46 μl of BICINE pH 7.4 (1 M)
90.54 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
F7
  • di-Sodium succinate 0.8 M -
NA
266.67 μl of di-Sodium succinate pH 7 (3 M)
733.33 μl of water (100 v/v)
F8
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate pH 7 (3 M)
300.0 μl of water (100 v/v)
F9
  • di-Sodium malonate 2.4 M -
NA
800.0 μl of di-Sodium malonate pH 7 (3 M)
200.0 μl of water (100 v/v)
F10
  • di-Sodium malonate 1.1 M -
  • Jeffamine® ED-2001 0.5 v/v -
  • HEPES 0.1 M pH 7
pH 7
366.67 μl of di-Sodium malonate pH 7 (3 M)
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Jeffamine® ED-2001 (50 v/v)
523.33 μl of water (100 v/v)
F11
  • di-Sodium succinate 1 M -
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
  • HEPES 0.1 M pH 7
pH 7
333.33 μl of di-Sodium succinate pH 7 (3 M)
100.0 μl of HEPES pH 7 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
546.67 μl of water (100 v/v)
F12
  • Jeffamine® M-600 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
G1
  • Jeffamine® ED-2001 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® ED-2001 (50 v/v)
300.0 μl of water (100 v/v)
G2
  • Poly(acrylic acid sodium salt) 5,100 22 w/v -
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Magnesium chloride (2 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
450.0 μl of water (100 v/v)
G3
  • Polyvinylpyrrolidone K15 20 w/v -
  • Cobalt (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
10.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
G4
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,350 12 w/v -
  • Cobalt (II) chloride 0.005 M -
  • Cadmium chloride 0.005 M -
  • Magnesium chloride 0.005 M -
  • Nickel (II) chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
5.0 μl of Cadmium chloride (1 M)
5.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
2.5 μl of Magnesium chloride (2 M)
1.25 μl of Nickel (II) chloride (4 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
646.25 μl of water (100 v/v)
G6
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.2 M -
NA
66.67 μl of di-Sodium malonate pH 7 (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
533.33 μl of water (100 v/v)
G7
  • Polyethylene glycol 3,350 15 w/v -
  • di-Sodium succinate 0.1 M -
NA
33.33 μl of di-Sodium succinate pH 7 (3 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
666.67 μl of water (100 v/v)
G8
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium DL-malate 0.15 M -
NA
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
G9
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium thiocyanate 0.1 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
G10
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
G11
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
328.57 μl of water (100 v/v)
G12
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
H1
  • Magnesium formate 0.3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Magnesium formate (1 M)
600.0 μl of water (100 v/v)
H2
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 3,350 1 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
285.71 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
594.29 μl of water (100 v/v)
H3
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
H4
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 10,000 17 w/v -
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
57.14 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.86 μl of water (100 v/v)
H8
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of water (100 v/v)