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Supplemental Information

Screen Description

MacroSol

Molecular Dimensions / MacroSol

Created 2009-11-16.

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MacroSol

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MacroSol

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Screen Structure

MacroSol

Well Count	48
Number of distinct ingredients	22
Internal diversity (score mean dist)	0.904

Screen Composition

More information about screen composition chart.

ingredient type	MacroSol
Buffer	54
Organic (non-volatile)	8
Organic (volatile)	8
Polymer	29
Salt	70

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

MacroSol Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	2-methyl-2,4-pentanediol BCC 10 v/v - Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 790.0 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 20 v/v - Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 690.0 μl of water (100 v/v)
A3	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 30 v/v - Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 590.0 μl of water (100 v/v)
A5	Sodium acetate 1 M - Sodium di-hydrogen phosphate 0.1 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	333.3 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 20.0 μl of Sodium di-hydrogen phosphate (5 M) 546.7 μl of water (100 v/v)
A6	Sodium acetate 1.4 M - Sodium di-hydrogen phosphate 0.1 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	466.7 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 20.0 μl of Sodium di-hydrogen phosphate (5 M) 413.3 μl of water (100 v/v)
A7	Sodium acetate 1.7 M - Sodium di-hydrogen phosphate 0.1 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	566.7 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 20.0 μl of Sodium di-hydrogen phosphate (5 M) 313.3 μl of water (100 v/v)
A8	Sodium acetate 1.7 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	566.7 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 333.3 μl of water (100 v/v)
B1	Polyethylene glycol 3,350 8 w/v - Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of Ammonium acetate (1 M) 160.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 540.0 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 15 w/v - Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of Ammonium acetate (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 400.0 μl of water (100 v/v)
B3	Polyethylene glycol 3,350 20 w/v - Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of Ammonium acetate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 300.0 μl of water (100 v/v)
B4	Polyethylene glycol 3,350 30 w/v - Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of Ammonium acetate (1 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 100.0 μl of water (100 v/v)
B5	Ammonium di-hydrogen phosphate 0.75 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	300.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 637.5 μl of water (100 v/v)
B6	Ammonium di-hydrogen phosphate 1 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	400.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 537.5 μl of water (100 v/v)
B7	Ammonium di-hydrogen phosphate 1.5 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	600.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 337.5 μl of water (100 v/v)
B8	Ammonium di-hydrogen phosphate 2 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	800.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 137.5 μl of water (100 v/v)
C1	2-Propanol 10 v/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 700.0 μl of water (100 v/v)
C2	2-Propanol 15 v/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	150.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 650.0 μl of water (100 v/v)
C3	2-Propanol 20 v/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 600.0 μl of water (100 v/v)
C4	2-Propanol 30 v/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	300.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of water (100 v/v)
C5	Polyethylene glycol 3,350 16 w/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of Lithium sulfate (2 M) 320.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 480.0 μl of water (100 v/v)
C6	Polyethylene glycol 3,350 25 w/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
C7	Polyethylene glycol 3,350 30 w/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of Lithium sulfate (2 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v)
C8	Polyethylene glycol 3,350 25 w/v - Lithium sulfate 0.5 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	250.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 150.0 μl of water (100 v/v)
D1	Polyethylene glycol 8,000 2 w/v - Lithium sulfate 1 M - 🔍	NA	500.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 460.0 μl of water (100 v/v)
D2	Polyethylene glycol 8,000 2 w/v - Lithium sulfate 1 M - Imidazole-Malate 0.1 M pH 5.5 🔍	pH 5.5	50.0 μl of Imidazole-Malate pH 5.5 (2 M) 500.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 410.0 μl of water (100 v/v)
D3	Polyethylene glycol 8,000 2 w/v - Lithium sulfate 1 M - Imidazole-Malate 0.1 M pH 6.5 🔍	pH 6.5	45.5 μl of Imidazole-Malate pH 5.5 (2 M) 4.5 μl of Imidazole-Malate pH 7.5 (2 M) 500.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 410.0 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 2 w/v - Lithium sulfate 1 M - Imidazole-Malate 0.1 M pH 7.5 🔍	pH 7.5	50.0 μl of Imidazole-Malate pH 7.5 (2 M) 500.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 410.0 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 12 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 702.9 μl of water (100 v/v)
D6	Polyethylene glycol 8,000 18 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 582.9 μl of water (100 v/v)
D7	Polyethylene glycol 8,000 24 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 480.0 μl of Polyethylene glycol 8,000 (50 w/v) 462.9 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 30 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 342.9 μl of water (100 v/v)
E1	Polyethylene glycol 4,000 10 w/v - Ammonium sulfate 0.5 M - 🔍	NA	142.9 μl of Ammonium sulfate (3.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 657.1 μl of water (100 v/v)
E2	Polyethylene glycol 4,000 20 w/v - Ammonium sulfate 0.3 M - 🔍	NA	85.7 μl of Ammonium sulfate (3.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 514.3 μl of water (100 v/v)
E3	Polyethylene glycol 4,000 30 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 342.9 μl of water (100 v/v)
E4	Polyethylene glycol 4,000 36 w/v - Ammonium sulfate 0.2 M - 🔍	NA	57.1 μl of Ammonium sulfate (3.5 M) 720.0 μl of Polyethylene glycol 4,000 (50 w/v) 222.9 μl of water (100 v/v)
E5	Ammonium sulfate 1 M - Polyethylene glycol 400 2 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	285.7 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 594.3 μl of water (100 v/v)
E6	Ammonium sulfate 1.5 M - Polypropylene glycol 400 2 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	428.6 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polypropylene glycol 400 (100 v/v) 451.4 μl of water (100 v/v)
E7	Ammonium sulfate 2 M - Polypropylene glycol 400 2 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polypropylene glycol 400 (100 v/v) 308.6 μl of water (100 v/v)
E8	Ammonium sulfate 2 M - Polypropylene glycol 400 5 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Polypropylene glycol 400 (100 v/v) 278.6 μl of water (100 v/v)
F1	Polyethylene glycol 4,000 12 w/v - 2-Propanol 5 v/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	50.0 μl of 2-Propanol (100 v/v) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 647.5 μl of water (100 v/v)
F2	Polyethylene glycol 4,000 16 w/v - 2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	100.0 μl of 2-Propanol (100 v/v) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 517.5 μl of water (100 v/v)
F3	Polyethylene glycol 4,000 20 w/v - 2-Propanol 15 v/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	150.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 387.5 μl of water (100 v/v)
F4	Polyethylene glycol 4,000 20 w/v - 2-Propanol 20 v/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	200.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 337.5 μl of water (100 v/v)
F5	Polyethylene glycol 8,000 9 w/v - Zinc acetate 0.005 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	180.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 5.0 μl of Zinc acetate (1 M) 715.0 μl of water (100 v/v)
F6	Polyethylene glycol 8,000 12 w/v - Zinc acetate 0.005 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	240.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 5.0 μl of Zinc acetate (1 M) 655.0 μl of water (100 v/v)
F7	Polyethylene glycol 8,000 18 w/v - Zinc acetate 0.005 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 5.0 μl of Zinc acetate (1 M) 535.0 μl of water (100 v/v)
F8	Polyethylene glycol 8,000 24 w/v - Zinc acetate 0.005 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	480.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 5.0 μl of Zinc acetate (1 M) 415.0 μl of water (100 v/v)