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Supplemental Information

Screen Description

MacroSol

Created 2009-11-16.

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MacroSol

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MacroSol

    Screen Structure

    MacroSol

    Well Count 48
    Number of distinct ingredients 22
    Internal diversity (score mean dist) 0.904

    Screen Composition

    More information about screen composition chart.
    2025-06-14T12:57:24.307584 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MacroSol
    Buffer 54
    Organic (non-volatile) 8
    Organic (volatile) 8
    Polymer 29
    Salt 70

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MacroSol Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    10 μl of Calcium chloride (2 M)
    58 μl of Sodium acetate pH 4 (1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    41 μl of Sodium acetate pH 5 (1 M)
    790 μl of water (100 v/v)
    A2
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    10 μl of Calcium chloride (2 M)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    41 μl of Sodium acetate pH 5 (1 M)
    690 μl of water (100 v/v)
    A3
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    58 μl of Sodium acetate pH 4 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    41 μl of Sodium acetate pH 5 (1 M)
    600 μl of water (100 v/v)
    A4
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    10 μl of Calcium chloride (2 M)
    58 μl of Sodium acetate pH 4 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    41 μl of Sodium acetate pH 5 (1 M)
    590 μl of water (100 v/v)
    A5
    • Sodium acetate 1 M -
    • Sodium di-hydrogen phosphate 0.1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    333 μl of Sodium acetate (3 M)
    20 μl of Sodium di-hydrogen phosphate (5 M)
    546 μl of water (100 v/v)
    A6
    • Sodium acetate 1.4 M -
    • Sodium di-hydrogen phosphate 0.1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    466 μl of Sodium acetate (3 M)
    20 μl of Sodium di-hydrogen phosphate (5 M)
    413 μl of water (100 v/v)
    A7
    • Sodium acetate 1.7 M -
    • Sodium di-hydrogen phosphate 0.1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    566 μl of Sodium acetate (3 M)
    20 μl of Sodium di-hydrogen phosphate (5 M)
    313 μl of water (100 v/v)
    A8
    • Sodium acetate 1.7 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    566 μl of Sodium acetate (3 M)
    333 μl of water (100 v/v)
    B1
    • Polyethylene glycol 3,350 8 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of Ammonium acetate (1 M)
    41 μl of Sodium acetate pH 5 (1 M)
    540 μl of water (100 v/v)
    B2
    • Polyethylene glycol 3,350 15 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of Ammonium acetate (1 M)
    41 μl of Sodium acetate pH 5 (1 M)
    400 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of Ammonium acetate (1 M)
    41 μl of Sodium acetate pH 5 (1 M)
    300 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 30 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of Ammonium acetate (1 M)
    41 μl of Sodium acetate pH 5 (1 M)
    100 μl of water (100 v/v)
    B5
    • Ammonium di-hydrogen phosphate 0.75 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    300 μl of Ammonium di-hydrogen phosphate (2.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    637 μl of water (100 v/v)
    B6
    • Ammonium di-hydrogen phosphate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    537 μl of water (100 v/v)
    B7
    • Ammonium di-hydrogen phosphate 1.5 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    600 μl of Ammonium di-hydrogen phosphate (2.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    337 μl of water (100 v/v)
    B8
    • Ammonium di-hydrogen phosphate 2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    137 μl of water (100 v/v)
    C1
    • 2-Propanol 10 v/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    700 μl of water (100 v/v)
    C2
    • 2-Propanol 15 v/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of 2-Propanol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    650 μl of water (100 v/v)
    C3
    • 2-Propanol 20 v/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of 2-Propanol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    600 μl of water (100 v/v)
    C4
    • 2-Propanol 30 v/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    100 μl of Magnesium chloride (2 M)
    500 μl of water (100 v/v)
    C5
    • Polyethylene glycol 3,350 16 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    480 μl of water (100 v/v)
    C6
    • Polyethylene glycol 3,350 25 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    C7
    • Polyethylene glycol 3,350 30 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    C8
    • Polyethylene glycol 3,350 25 w/v -
    • Lithium sulfate 0.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    D1
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    		NA
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of Lithium sulfate (2 M)
    460 μl of water (100 v/v)
    D2
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    • Imidazole-Malate 0.1 M pH 5.5
    		pH 5.5
    50 μl of Imidazole-Malate pH 5.5 (2 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of Lithium sulfate (2 M)
    410 μl of water (100 v/v)
    D3
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    • Imidazole-Malate 0.1 M pH 6.5
    		pH 6.5
    50 μl of Imidazole-Malate pH 5.5 (2 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    0 μl of Imidazole-Malate pH 7.5 (2 M)
    500 μl of Lithium sulfate (2 M)
    410 μl of water (100 v/v)
    D4
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    • Imidazole-Malate 0.1 M pH 7.5
    		pH 7.5
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Imidazole-Malate pH 7.5 (2 M)
    500 μl of Lithium sulfate (2 M)
    410 μl of water (100 v/v)
    D5
    • Polyethylene glycol 8,000 12 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    702 μl of water (100 v/v)
    D6
    • Polyethylene glycol 8,000 18 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    582 μl of water (100 v/v)
    D7
    • Polyethylene glycol 8,000 24 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    480 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    462 μl of water (100 v/v)
    D8
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    342 μl of water (100 v/v)
    E1
    • Polyethylene glycol 4,000 10 w/v -
    • Ammonium sulfate 0.5 M -
    		NA
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    142 μl of Ammonium sulfate (3.5 M)
    657 μl of water (100 v/v)
    E2
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium sulfate 0.3 M -
    		NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of Ammonium sulfate (3.5 M)
    514 μl of water (100 v/v)
    E3
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    342 μl of water (100 v/v)
    E4
    • Polyethylene glycol 4,000 36 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    720 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    222 μl of water (100 v/v)
    E5
    • Ammonium sulfate 1 M -
    • Polyethylene glycol 400 2 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    594 μl of water (100 v/v)
    E6
    • Ammonium sulfate 1.5 M -
    • Polypropylene glycol 400 2 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    20 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    451 μl of water (100 v/v)
    E7
    • Ammonium sulfate 2 M -
    • Polypropylene glycol 400 2 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    20 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    308 μl of water (100 v/v)
    E8
    • Ammonium sulfate 2 M -
    • Polypropylene glycol 400 5 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    50 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    278 μl of water (100 v/v)
    F1
    • Polyethylene glycol 4,000 12 w/v -
    • 2-Propanol 5 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    50 μl of 2-Propanol (100 v/v)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    647 μl of water (100 v/v)
    F2
    • Polyethylene glycol 4,000 16 w/v -
    • 2-Propanol 10 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    100 μl of 2-Propanol (100 v/v)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    517 μl of water (100 v/v)
    F3
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 15 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    150 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    387 μl of water (100 v/v)
    F4
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    200 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    337 μl of water (100 v/v)
    F5
    • Polyethylene glycol 8,000 9 w/v -
    • Zinc acetate 0.005 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    5 μl of Zinc acetate (1 M)
    715 μl of water (100 v/v)
    F6
    • Polyethylene glycol 8,000 12 w/v -
    • Zinc acetate 0.005 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    5 μl of Zinc acetate (1 M)
    655 μl of water (100 v/v)
    F7
    • Polyethylene glycol 8,000 18 w/v -
    • Zinc acetate 0.005 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    5 μl of Zinc acetate (1 M)
    535 μl of water (100 v/v)
    F8
    • Polyethylene glycol 8,000 24 w/v -
    • Zinc acetate 0.005 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    480 μl of Polyethylene glycol 8,000 (50 w/v)
    5 μl of Zinc acetate (1 M)
    415 μl of water (100 v/v)