C6 - Screen Contents

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Supplemental Information

Screen Description

MemPlus

Molecular Dimensions / MemPlus

Created 2009-11-16.

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MemPlus

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MemPlus

Screen Structure

MemPlus

Well Count	48
Number of distinct ingredients	54
Internal diversity (score mean dist)	0.959

Screen Composition

More information about screen composition chart.

ingredient type	MemPlus
Buffer	56
Organic (non-volatile)	12
Organic (volatile)	2
Polymer	37
Salt	57

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

MemPlus Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.1 M pH 5 Polyethylene glycol 300 30 v/v - 🔍	pH 5	300 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium acetate pH 5 (1 M) 600 μl of water (100 v/v)
A2	Cadmium chloride 0.2 M - HEPES 0.1 M pH 7 Polyethylene glycol 400 15 v/v - 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 150 μl of Polyethylene glycol 400 (100 v/v) 200 μl of Cadmium chloride (1 M) 550 μl of water (100 v/v)
A3	Ammonium sulfate 1.65 M - tris chloride BCC 0.1 M pH 8 Polyethylene glycol 400 2 v/v - 🔍	pH 8	47 μl of tris chloride BCC pH 8.5 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 53 μl of tris chloride BCC pH 7.5 (1 M) 471 μl of Ammonium sulfate (3.5 M) 408 μl of water (100 v/v)
A4	Sodium formate 1.5 M - Sodium cacodylate 0.05 M pH 5.5 Polyethylene glycol 400 30 v/v - 🔍	pH 5.5	300 μl of Polyethylene glycol 400 (100 v/v) 50 μl of Sodium cacodylate pH 5.5 (1 M) 214 μl of Sodium formate (7 M) 435 μl of water (100 v/v)
A5	Calcium chloride 0.2 M - Glycine 0.05 M pH 9 Polyethylene glycol 400 30 v/v - 🔍	pH 9	50 μl of Glycine pH 9 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Calcium chloride (2 M) 550 μl of water (100 v/v)
A6	Sodium acetate 0.05 M pH 4.3 Polyethylene glycol monomethyl ether 550 33 v/v - 🔍	pH 4.3	10 μl of Sodium acetate pH 5 (1 M) 330 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 39 μl of Sodium acetate pH 4 (1 M) 620 μl of water (100 v/v)
A7	Potassium chloride 0.2 M - tris chloride BCC 0.02 M pH 7.5 Polyethylene glycol 600 30 v/v - Calcium chloride 0.01 M - 🔍	pH 7.5	50 μl of Potassium chloride (4 M) 20 μl of tris chloride BCC pH 7.5 (1 M) 5 μl of Calcium chloride (2 M) 300 μl of Polyethylene glycol 600 (100 v/v) 625 μl of water (100 v/v)
A8	Lithium sulfate 1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 600 20 v/v - 🔍	pH 7.5	500 μl of Lithium sulfate (2 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 600 (100 v/v) 200 μl of water (100 v/v)
B1	Lithium chloride 0.3 M - tris chloride BCC 0.02 M pH 6.8 Polyethylene glycol 600 35 v/v - 🔍	pH 6.8	9 μl of tris chloride BCC pH 7 (1 M) 30 μl of Lithium chloride (10 M) 10 μl of tris chloride BCC pH 6.5 (1 M) 350 μl of Polyethylene glycol 600 (100 v/v) 600 μl of water (100 v/v)
B2	HEPES 0.06 M pH 7 Polyethylene glycol 1,000 28 w/v - tris chloride BCC 0.04 M pH 7 🔍	NA	560 μl of Polyethylene glycol 1,000 (50 w/v) 40 μl of tris chloride BCC pH 7 (1 M) 60 μl of HEPES pH 7 (1 M) 340 μl of water (100 v/v)
B3	Sodium chloride 0.35 M - TRICINE 0.1 M pH 8 Polyethylene glycol 1,000 31 w/v - 🔍	pH 8	620 μl of Polyethylene glycol 1,000 (50 w/v) 41 μl of TRICINE pH 8.8 (1 M) 70 μl of Sodium chloride (5 M) 58 μl of TRICINE pH 7.4 (1 M) 210 μl of water (100 v/v)
B4	Lithium sulfate 0.2 M - tri-Sodium citrate 0.1 M pH 4 Polyethylene glycol 1,000 9 w/v - 🔍	pH 4	100 μl of Lithium sulfate (2 M) 180 μl of Polyethylene glycol 1,000 (50 w/v) 10 μl of tri-Sodium citrate pH 3 (1.6 M) 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 657 μl of water (100 v/v)
B5	Sodium chloride 0.35 M - MOPS 0.0125 M pH 7 Polyethylene glycol 1,000 28 w/v - 🔍	pH 7	560 μl of Polyethylene glycol 1,000 (50 w/v) 8 μl of MOPS pH 6.5 (1 M) 70 μl of Sodium chloride (5 M) 4 μl of MOPS pH 7.9 (1 M) 357 μl of water (100 v/v)
B6	tris chloride BCC 0.02 M pH 7.5 Polyethylene glycol 1,500 33 w/v - 🔍	pH 7.5	20 μl of tris chloride BCC pH 7.5 (1 M) 660 μl of Polyethylene glycol 1,500 (50 w/v) 320 μl of water (100 v/v)
B7	Sodium chloride 0.1 M - EPPS 0.1 M pH 8 Polyethylene glycol 1,500 33 w/v - 🔍	pH 8	20 μl of Sodium chloride (5 M) 100 μl of EPPS pH 8 (1 M) 660 μl of Polyethylene glycol 1,500 (50 w/v) 220 μl of water (100 v/v)
B8	Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 2,000 12 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 240 μl of Polyethylene glycol 2,000 (50 w/v) 660 μl of water (100 v/v)
C1	Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 2,000 12.5 w/v - 🔍	pH 6.5	250 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 650 μl of water (100 v/v)
C2	tris chloride BCC 0.02 M pH 7.5 Polyethylene glycol monomethyl ether 2,000 12.5 w/v - 🔍	pH 7.5	250 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 20 μl of tris chloride BCC pH 7.5 (1 M) 730 μl of water (100 v/v)
C3	Sodium chloride 0.4 M - tris chloride BCC 0.02 M pH 7.5 Polyethylene glycol monomethyl ether 2,000 12.5 w/v - Magnesium chloride 0.025 M - 🔍	pH 7.5	250 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12 μl of Magnesium chloride (2 M) 80 μl of Sodium chloride (5 M) 20 μl of tris chloride BCC pH 7.5 (1 M) 637 μl of water (100 v/v)
C4	Ammonium di-hydrogen phosphate 0.2 M - PIPES 0.05 M pH 7 Polyethylene glycol 2,000 20 w/v - 🔍	pH 7	50 μl of PIPES pH 7 (1 M) 400 μl of Polyethylene glycol 2,000 (50 w/v) 80 μl of Ammonium di-hydrogen phosphate (2.5 M) 470 μl of water (100 v/v)
C5	Sodium chloride 0.5 M - tris chloride BCC 0.025 M pH 8 Polyethylene glycol 2,000 28 w/v - 🔍	pH 8	11 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Sodium chloride (5 M) 560 μl of Polyethylene glycol 2,000 (50 w/v) 13 μl of tris chloride BCC pH 7.5 (1 M) 315 μl of water (100 v/v)
C6	Magnesium chloride 0.5 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol 2,000 15 w/v - 🔍	pH 8.5	50 μl of tris chloride BCC pH 8.5 (1 M) 250 μl of Magnesium chloride (2 M) 300 μl of Polyethylene glycol 2,000 (50 w/v) 400 μl of water (100 v/v)
C7	Magnesium chloride 0.3 M - BICINE 0.1 M pH 9 Polyethylene glycol 2,000 28 w/v - 🔍	pH 9	150 μl of Magnesium chloride (2 M) 90 μl of BICINE pH 9.3 (1 M) 560 μl of Polyethylene glycol 2,000 (50 w/v) 9 μl of BICINE pH 7.4 (1 M) 190 μl of water (100 v/v)
C8	Lithium sulfate 0.1 M - ADA 0.1 M pH 6.6 Polyethylene glycol 3,000 13 w/v - 🔍	pH 6.6	50 μl of Lithium sulfate (2 M) 58 μl of ADA pH 7 (1 M) 41 μl of ADA pH 6 (1 M) 260 μl of Polyethylene glycol 3,000 (50 w/v) 590 μl of water (100 v/v)
D1	Calcium acetate 0.01 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,000 3 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 60 μl of Polyethylene glycol 3,000 (50 w/v) 10 μl of Calcium acetate (1 M) 830 μl of water (100 v/v)
D2	Magnesium acetate 0.2 M - MES 0.1 M pH 6 Polyethylene glycol 3,350 10 w/v - 🔍	pH 6	83 μl of MES pH 7.1 (0.5 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 117 μl of MES pH 5.2 (0.5 M) 200 μl of Magnesium acetate (1 M) 400 μl of water (100 v/v)
D3	Magnesium acetate 0.2 M - Sodium cacodylate 0.05 M pH 6.5 Polyethylene glycol 3,350 11 w/v - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 220 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium acetate (1 M) 530 μl of water (100 v/v)
D4	Magnesium acetate 0.2 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 3,350 11 w/v - 🔍	pH 6.5	100 μl of BIS-TRIS pH 6.5 (1 M) 220 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium acetate (1 M) 480 μl of water (100 v/v)
D5	Lithium chloride 0.1 M - tris chloride BCC 0.01 M pH 7.5 Polyethylene glycol 4,000 14 w/v - Magnesium chloride 0.025 M - 🔍	pH 7.5	12 μl of Magnesium chloride (2 M) 10 μl of Lithium chloride (10 M) 10 μl of tris chloride BCC pH 7.5 (1 M) 280 μl of Polyethylene glycol 4,000 (50 w/v) 687 μl of water (100 v/v)
D6	Ammonium sulfate 0.2 M - CHES 0.1 M pH 10 Polyethylene glycol 4,000 14 w/v - 🔍	pH 10	100 μl of CHES pH 10 (1 M) 57 μl of Ammonium sulfate (3.5 M) 280 μl of Polyethylene glycol 4,000 (50 w/v) 562 μl of water (100 v/v)
D7	Potassium Sodium tartrate 0.15 M - ADA 0.05 M pH 6.6 Polyethylene glycol 4,000 20 w/v - 🔍	pH 6.6	29 μl of ADA pH 7 (1 M) 20 μl of ADA pH 6 (1 M) 100 μl of Potassium Sodium tartrate (1.5 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 450 μl of water (100 v/v)
D8	Sodium chloride 0.7 M - Sodium di-hydrogen phosphate 0.1 M pH 7 Polyethylene glycol 4,000 25 w/v - 🔍	pH 7	8 μl of Sodium di-hydrogen phosphate pH 8 (5 M) 11 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M) 140 μl of Sodium chloride (5 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 340 μl of water (100 v/v)
E1	Zinc acetate 0.15 M - tris chloride BCC 0.05 M pH 7.5 Polyethylene glycol 6,000 13 w/v - Zinc chloride 0.05 M - 🔍	pH 7.5	260 μl of Polyethylene glycol 6,000 (50 w/v) 25 μl of Zinc chloride (2 M) 50 μl of tris chloride BCC pH 7.5 (1 M) 150 μl of Zinc acetate (1 M) 515 μl of water (100 v/v)
E2	Ammonium chloride 0.2 M - TRICINE 0.15 M pH 8 Polyethylene glycol 6,000 15 w/v - 🔍	pH 8	300 μl of Polyethylene glycol 6,000 (50 w/v) 62 μl of TRICINE pH 8.8 (1 M) 40 μl of Ammonium chloride (5 M) 87 μl of TRICINE pH 7.4 (1 M) 510 μl of water (100 v/v)
E3	Potassium Sodium phosphate 0.025 M pH 5.1 Polyethylene glycol 8,000 13 w/v - 🔍	pH 5.1	8 μl of Potassium Sodium phosphate pH 5.1 (3 M) 260 μl of Polyethylene glycol 8,000 (50 w/v) 731 μl of water (100 v/v)
E4	Zinc acetate 0.15 M - Sodium cacodylate 0.08 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	80 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 150 μl of Zinc acetate (1 M) 470 μl of water (100 v/v)
E5	Magnesium acetate 0.1 M - PIPES 0.1 M pH 6.8 Polyethylene glycol 8,000 30 w/v - 🔍	pH 6.8	76 μl of PIPES pH 7 (1 M) 23 μl of PIPES pH 6 (1 M) 100 μl of Magnesium acetate (1 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of water (100 v/v)
E6	Ammonium sulfate 1.4 M - 2-Propanol 4 v/v - Ammonium acetate 0.1 M - 🔍	NA	100 μl of Ammonium acetate (1 M) 40 μl of 2-Propanol (100 v/v) 400 μl of Ammonium sulfate (3.5 M) 460 μl of water (100 v/v)
E7	Sodium acetate 0.5 M - Imidazole 0.1 M pH 8 2-methyl-2,4-pentanediol BCC 25 v/v - tris chloride BCC 0 M pH 8 🔍	NA	0 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Imidazole pH 8 (1 M) 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 0 μl of tris chloride BCC pH 7.5 (1 M) 166 μl of Sodium acetate (3 M) 483 μl of water (100 v/v)
E8	Calcium chloride 0.001 M - BIS-TRIS 0.1 M pH 6 2-methyl-2,4-pentanediol BCC 27 v/v - 🔍	pH 6	36 μl of BIS-TRIS pH 6.5 (1 M) 270 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 0 μl of Calcium chloride (2 M) 63 μl of BIS-TRIS pH 5.5 (1 M) 629 μl of water (100 v/v)
F1	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 5.5	200 μl of Ammonium acetate (1 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 58 μl of tri-Sodium citrate pH 6.5 (1.6 M) 437 μl of water (100 v/v)
F2	Calcium chloride 0.2 M - 2-Propanol 30 v/v - 🔍	NA	300 μl of 2-Propanol (100 v/v) 100 μl of Calcium chloride (2 M) 600 μl of water (100 v/v)
F3	Ammonium sulfite 1.3 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 650 μl of Ammonium sulfite (2 M) 250 μl of water (100 v/v)
F4	Sodium chloride 0.5 M - tri-Sodium citrate 0.1 M pH 4.5 2-methyl-2,4-pentanediol BCC 28 v/v - 🔍	pH 4.5	8 μl of tri-Sodium citrate pH 3 (1.6 M) 100 μl of Sodium chloride (5 M) 280 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 54 μl of tri-Sodium citrate pH 6.5 (1.6 M) 557 μl of water (100 v/v)
F5	Sodium chloride 0.2 M - HEPES 0.1 M pH 7 2-methyl-2,4-pentanediol BCC 35 v/v - 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 40 μl of Sodium chloride (5 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 510 μl of water (100 v/v)
F6	Calcium chloride 0.3 M - PIPES 0.1 M pH 6.5 🔍	pH 6.5	41 μl of PIPES pH 7 (1 M) 58 μl of PIPES pH 6 (1 M) 150 μl of Calcium chloride (2 M) 750 μl of water (100 v/v)
F7	Ammonium sulfate 0.1 M - Glycine 0.1 M pH 3.8 Triethylene glycol 25 w/v - 🔍	pH 3.8	50 μl of Glycine pH 3.8 (2 M) 250 μl of Triethylene glycol (100 w/v) 28 μl of Ammonium sulfate (3.5 M) 671 μl of water (100 v/v)
F8	Magnesium chloride 0.15 M - EPPS 0.05 M pH 8 Barium chloride 14 w/v - 🔍	pH 8	466 μl of Barium chloride (30 w/v) 75 μl of Magnesium chloride (2 M) 50 μl of EPPS pH 8 (1 M) 408 μl of water (100 v/v)