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Supplemental Information

Screen Description

MemPlus

Created 2009-11-16.

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MemPlus

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Screen Structure

MemPlus

Well Count 48
Number of distinct ingredients 54
Internal diversity (score mean dist) 0.959

Screen Composition

2025-08-16T06:25:41.186549 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemPlus
Buffer 56
Organic (non-volatile) 12
Organic (volatile) 2
Polymer 37
Salt 57

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemPlus Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 5
  • Polyethylene glycol 300 30 v/v -
pH 5
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
A2
  • Cadmium chloride 0.2 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 15 v/v -
pH 7
200.0 μl of Cadmium chloride (1 M)
100.0 μl of HEPES pH 7 (1 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 1.65 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 2 v/v -
pH 8
471.4 μl of Ammonium sulfate (3.5 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
408.6 μl of water (100 v/v)
A4
  • Sodium formate 1.5 M -
  • Sodium cacodylate 0.05 M pH 5.5
  • Polyethylene glycol 400 30 v/v -
pH 5.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium cacodylate pH 5.5 (1 M)
214.3 μl of Sodium formate (7 M)
435.7 μl of water (100 v/v)
A5
  • Calcium chloride 0.2 M -
  • Glycine 0.05 M pH 9
  • Polyethylene glycol 400 30 v/v -
pH 9
100.0 μl of Calcium chloride (2 M)
50.0 μl of Glycine pH 9 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.05 M pH 4.3
  • Polyethylene glycol monomethyl ether 550 33 v/v -
pH 4.3
330.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
39.1 μl of Sodium acetate pH 4 (1 M)
10.9 μl of Sodium acetate pH 5 (1 M)
620.0 μl of water (100 v/v)
A7
  • Potassium chloride 0.2 M -
  • tris chloride BCC 0.02 M pH 7.5
  • Polyethylene glycol 600 30 v/v -
  • Calcium chloride 0.01 M -
pH 7.5
5.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
50.0 μl of Potassium chloride (4 M)
20.0 μl of tris chloride BCC pH 7.5 (1 M)
625.0 μl of water (100 v/v)
A8
  • Lithium sulfate 1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 20 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 600 (100 v/v)
200.0 μl of water (100 v/v)
B1
  • Lithium chloride 0.3 M -
  • tris chloride BCC 0.02 M pH 6.8
  • Polyethylene glycol 600 35 v/v -
pH 6.8
30.0 μl of Lithium chloride (10 M)
350.0 μl of Polyethylene glycol 600 (100 v/v)
10.5 μl of tris chloride BCC pH 6.5 (1 M)
9.5 μl of tris chloride BCC pH 7 (1 M)
600.0 μl of water (100 v/v)
B2
  • HEPES 0.06 M pH 7
  • Polyethylene glycol 1,000 28 w/v -
  • tris chloride BCC 0.04 M pH 7
NA
60.0 μl of HEPES pH 7 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
40.0 μl of tris chloride BCC pH 7 (1 M)
340.0 μl of water (100 v/v)
B3
  • Sodium chloride 0.35 M -
  • TRICINE 0.1 M pH 8
  • Polyethylene glycol 1,000 31 w/v -
pH 8
620.0 μl of Polyethylene glycol 1,000 (50 w/v)
70.0 μl of Sodium chloride (5 M)
58.6 μl of TRICINE pH 7.4 (1 M)
41.4 μl of TRICINE pH 8.8 (1 M)
210.0 μl of water (100 v/v)
B4
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 1,000 9 w/v -
pH 4
100.0 μl of Lithium sulfate (2 M)
180.0 μl of Polyethylene glycol 1,000 (50 w/v)
39.4 μl of tri-Sodium citrate pH 3 (1.6 M)
23.1 μl of tri-Sodium citrate pH 6.5 (1.6 M)
657.5 μl of water (100 v/v)
B5
  • Sodium chloride 0.35 M -
  • MOPS 0.0125 M pH 7
  • Polyethylene glycol 1,000 28 w/v -
pH 7
8.4 μl of MOPS pH 6.5 (1 M)
4.1 μl of MOPS pH 7.9 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
70.0 μl of Sodium chloride (5 M)
357.5 μl of water (100 v/v)
B6
  • tris chloride BCC 0.02 M pH 7.5
  • Polyethylene glycol 1,500 33 w/v -
pH 7.5
660.0 μl of Polyethylene glycol 1,500 (50 w/v)
20.0 μl of tris chloride BCC pH 7.5 (1 M)
320.0 μl of water (100 v/v)
B7
  • Sodium chloride 0.1 M -
  • EPPS 0.1 M pH 8
  • Polyethylene glycol 1,500 33 w/v -
pH 8
100.0 μl of EPPS pH 8 (1 M)
660.0 μl of Polyethylene glycol 1,500 (50 w/v)
20.0 μl of Sodium chloride (5 M)
220.0 μl of water (100 v/v)
B8
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 2,000 12 w/v -
pH 6.5
240.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
660.0 μl of water (100 v/v)
C1
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 12.5 w/v -
pH 6.5
250.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
650.0 μl of water (100 v/v)
C2
  • tris chloride BCC 0.02 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 12.5 w/v -
pH 7.5
250.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
20.0 μl of tris chloride BCC pH 7.5 (1 M)
730.0 μl of water (100 v/v)
C3
  • Sodium chloride 0.4 M -
  • tris chloride BCC 0.02 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 12.5 w/v -
  • Magnesium chloride 0.025 M -
pH 7.5
12.5 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
80.0 μl of Sodium chloride (5 M)
20.0 μl of tris chloride BCC pH 7.5 (1 M)
637.5 μl of water (100 v/v)
C4
  • Ammonium di-hydrogen phosphate 0.2 M -
  • PIPES 0.05 M pH 7
  • Polyethylene glycol 2,000 20 w/v -
pH 7
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
50.0 μl of PIPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
470.0 μl of water (100 v/v)
C5
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.025 M pH 8
  • Polyethylene glycol 2,000 28 w/v -
pH 8
560.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
13.2 μl of tris chloride BCC pH 7.5 (1 M)
11.8 μl of tris chloride BCC pH 8.5 (1 M)
315.0 μl of water (100 v/v)
C6
  • Magnesium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 2,000 15 w/v -
pH 8.5
250.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C7
  • Magnesium chloride 0.3 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 2,000 28 w/v -
pH 9
9.5 μl of BICINE pH 7.4 (1 M)
90.5 μl of BICINE pH 9.3 (1 M)
150.0 μl of Magnesium chloride (2 M)
560.0 μl of Polyethylene glycol 2,000 (50 w/v)
190.0 μl of water (100 v/v)
C8
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.6
  • Polyethylene glycol 3,000 13 w/v -
pH 6.6
41.8 μl of ADA pH 6 (1 M)
58.2 μl of ADA pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
260.0 μl of Polyethylene glycol 3,000 (50 w/v)
590.0 μl of water (100 v/v)
D1
  • Calcium acetate 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,000 3 w/v -
pH 8.5
10.0 μl of Calcium acetate (1 M)
60.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
830.0 μl of water (100 v/v)
D2
  • Magnesium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 3,350 10 w/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
200.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D3
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.05 M pH 6.5
  • Polyethylene glycol 3,350 11 w/v -
pH 6.5
200.0 μl of Magnesium acetate (1 M)
220.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium cacodylate pH 6.5 (1 M)
530.0 μl of water (100 v/v)
D4
  • Magnesium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 11 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
200.0 μl of Magnesium acetate (1 M)
220.0 μl of Polyethylene glycol 3,350 (50 w/v)
480.0 μl of water (100 v/v)
D5
  • Lithium chloride 0.1 M -
  • tris chloride BCC 0.01 M pH 7.5
  • Polyethylene glycol 4,000 14 w/v -
  • Magnesium chloride 0.025 M -
pH 7.5
10.0 μl of Lithium chloride (10 M)
12.5 μl of Magnesium chloride (2 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.0 μl of tris chloride BCC pH 7.5 (1 M)
687.5 μl of water (100 v/v)
D6
  • Ammonium sulfate 0.2 M -
  • CHES 0.1 M pH 10
  • Polyethylene glycol 4,000 14 w/v -
pH 10
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of CHES pH 10 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
562.9 μl of water (100 v/v)
D7
  • Potassium Sodium tartrate 0.15 M -
  • ADA 0.05 M pH 6.6
  • Polyethylene glycol 4,000 20 w/v -
pH 6.6
20.9 μl of ADA pH 6 (1 M)
29.1 μl of ADA pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Potassium Sodium tartrate (1.5 M)
450.0 μl of water (100 v/v)
D8
  • Sodium chloride 0.7 M -
  • Sodium di-hydrogen phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 25 w/v -
pH 7
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
140.0 μl of Sodium chloride (5 M)
11.5 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
8.5 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
340.0 μl of water (100 v/v)
E1
  • Zinc acetate 0.15 M -
  • tris chloride BCC 0.05 M pH 7.5
  • Polyethylene glycol 6,000 13 w/v -
  • Zinc chloride 0.05 M -
pH 7.5
260.0 μl of Polyethylene glycol 6,000 (50 w/v)
150.0 μl of Zinc acetate (1 M)
25.0 μl of Zinc chloride (2 M)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
515.0 μl of water (100 v/v)
E2
  • Ammonium chloride 0.2 M -
  • TRICINE 0.15 M pH 8
  • Polyethylene glycol 6,000 15 w/v -
pH 8
40.0 μl of Ammonium chloride (5 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
87.9 μl of TRICINE pH 7.4 (1 M)
62.1 μl of TRICINE pH 8.8 (1 M)
510.0 μl of water (100 v/v)
E3
  • Potassium Sodium phosphate 0.025 M pH 5.1
  • Polyethylene glycol 8,000 13 w/v -
pH 5.1
260.0 μl of Polyethylene glycol 8,000 (50 w/v)
8.3 μl of Potassium Sodium phosphate pH 5.1 (3 M)
731.7 μl of water (100 v/v)
E4
  • Zinc acetate 0.15 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 15 w/v -
pH 6.5
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
150.0 μl of Zinc acetate (1 M)
470.0 μl of water (100 v/v)
E5
  • Magnesium acetate 0.1 M -
  • PIPES 0.1 M pH 6.8
  • Polyethylene glycol 8,000 30 w/v -
pH 6.8
100.0 μl of Magnesium acetate (1 M)
23.8 μl of PIPES pH 6 (1 M)
76.2 μl of PIPES pH 7 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of water (100 v/v)
E6
  • Ammonium sulfate 1.4 M -
  • 2-Propanol 4 v/v -
  • Ammonium acetate 0.1 M -
NA
40.0 μl of 2-Propanol (100 v/v)
100.0 μl of Ammonium acetate (1 M)
400.0 μl of Ammonium sulfate (3.5 M)
460.0 μl of water (100 v/v)
E7
  • Sodium acetate 0.5 M -
  • Imidazole 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • tris chloride BCC 0 M pH 8
NA
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
166.7 μl of Sodium acetate (3 M)
0.0 μl of tris chloride BCC pH 7.5 (1 M)
0.0 μl of tris chloride BCC pH 8.5 (1 M)
483.3 μl of water (100 v/v)
E8
  • Calcium chloride 0.001 M -
  • BIS-TRIS 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 27 v/v -
pH 6
270.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
63.5 μl of BIS-TRIS pH 5.5 (1 M)
36.5 μl of BIS-TRIS pH 6.5 (1 M)
0.5 μl of Calcium chloride (2 M)
629.5 μl of water (100 v/v)
F1
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
437.5 μl of water (100 v/v)
F2
  • Calcium chloride 0.2 M -
  • 2-Propanol 30 v/v -
NA
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium chloride (2 M)
600.0 μl of water (100 v/v)
F3
  • Ammonium sulfite 1.3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
650.0 μl of Ammonium sulfite (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
F4
  • Sodium chloride 0.5 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • 2-methyl-2,4-pentanediol BCC 28 v/v -
pH 4.5
280.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium chloride (5 M)
35.3 μl of tri-Sodium citrate pH 3 (1.6 M)
27.2 μl of tri-Sodium citrate pH 6.5 (1.6 M)
557.5 μl of water (100 v/v)
F5
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 7
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7 (1 M)
40.0 μl of Sodium chloride (5 M)
510.0 μl of water (100 v/v)
F6
  • Calcium chloride 0.3 M -
  • PIPES 0.1 M pH 6.5
pH 6.5
150.0 μl of Calcium chloride (2 M)
58.6 μl of PIPES pH 6 (1 M)
41.4 μl of PIPES pH 7 (1 M)
750.0 μl of water (100 v/v)
F7
  • Ammonium sulfate 0.1 M -
  • Glycine 0.1 M pH 3.8
  • Triethylene glycol 25 w/v -
pH 3.8
28.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of Glycine pH 3.8 (2 M)
250.0 μl of Triethylene glycol (100 w/v)
671.4 μl of water (100 v/v)
F8
  • Magnesium chloride 0.15 M -
  • EPPS 0.05 M pH 8
  • Barium chloride 14 w/v -
pH 8
466.7 μl of Barium chloride (30 w/v)
50.0 μl of EPPS pH 8 (1 M)
75.0 μl of Magnesium chloride (2 M)
408.3 μl of water (100 v/v)