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Supplemental Information

Screen Description

MemStart

Created 2009-11-16.

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MemStart

    Screen Structure

    MemStart

    Well Count 48
    Number of distinct ingredients 25
    Internal diversity (score mean dist) 0.923

    Screen Composition

    2025-06-13T21:31:49.588546 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemStart
    Buffer 53
    Organic (non-volatile) 8
    Polymer 30
    Salt 65

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemStart Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    A2
    • ADA 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    pH 6.5
    100 μl of ADA pH 6.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    614 μl of water (100 v/v)
    A3
    • Ammonium sulfate 2 M -
    NA
    571 μl of Ammonium sulfate (3.5 M)
    428 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    pH 7.5
    750 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1 M -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    400 μl of Magnesium sulfate (2.5 M)
    500 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.1 M pH 5.6
    • Magnesium sulfate 1 M -
    pH 5.6
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Magnesium sulfate (2.5 M)
    537 μl of water (100 v/v)
    A8
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Magnesium sulfate 1 M -
    pH 6.5
    50 μl of Lithium sulfate (2 M)
    100 μl of ADA pH 6.5 (1 M)
    400 μl of Magnesium sulfate (2.5 M)
    450 μl of water (100 v/v)
    B1
    • Ammonium di-hydrogen phosphate 0.1 M pH 6.5
    pH 6.5
    40 μl of Ammonium di-hydrogen phosphate pH 6.5 (2.5 M)
    960 μl of water (100 v/v)
    B2
    • Ammonium sulfite 0.1 M -
    • Potassium di-hydrogen phosphate 0.5 M pH 7.5
    • Sodium di-hydrogen phosphate 0.5 M pH 7.5
    NA
    50 μl of Ammonium sulfite (2 M)
    23 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    250 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
    76 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    600 μl of water (100 v/v)
    B3
    • Lithium sulfate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium di-hydrogen phosphate 1 M -
    pH 4.6
    50 μl of Lithium sulfate (2 M)
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    450 μl of water (100 v/v)
    B4
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 1 M -
    pH 5.6
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    537 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 2 M -
    pH 8.5
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of water (100 v/v)
    B6
    • Sodium formate 2 M -
    NA
    285 μl of Sodium formate (7 M)
    714 μl of water (100 v/v)
    B7
    • Sodium formate 4 M -
    NA
    571 μl of Sodium formate (7 M)
    428 μl of water (100 v/v)
    B8
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    466 μl of Sodium acetate (3 M)
    333 μl of water (100 v/v)
    C1
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    875 μl of tri-Sodium citrate (1.6 M)
    25 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 1 M -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    666 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    233 μl of water (100 v/v)
    C3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 2 v/v -
    • Ammonium sulfate 2 M -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    571 μl of Ammonium sulfate (3.5 M)
    308 μl of water (100 v/v)
    C4
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    C5
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    pH 5.6
    20 μl of Sodium chloride (5 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    617 μl of water (100 v/v)
    C6
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    pH 5.6
    50 μl of Lithium sulfate (2 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    587 μl of water (100 v/v)
    C7
    • Lithium sulfate 0.3 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    150 μl of Lithium sulfate (2 M)
    100 μl of ADA pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    C8
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    50 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    D1
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    28 μl of Ammonium sulfate (3.5 M)
    571 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    125 μl of tri-Sodium citrate (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    475 μl of water (100 v/v)
    D3
    • Zinc acetate 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Zinc acetate (1 M)
    560 μl of water (100 v/v)
    D4
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    602 μl of water (100 v/v)
    D5
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    660 μl of water (100 v/v)
    D6
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 5.6
    50 μl of Lithium sulfate (2 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    647 μl of water (100 v/v)
    D7
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 12 w/v -
    pH 5.6
    20 μl of Sodium chloride (5 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    677 μl of water (100 v/v)
    D8
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    50 μl of Lithium sulfate (2 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of ADA pH 6.5 (1 M)
    610 μl of water (100 v/v)
    E1
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    20 μl of Sodium chloride (5 M)
    0 μl of HEPES pH 5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    640 μl of water (100 v/v)
    E2
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    0 μl of HEPES pH 5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    631 μl of water (100 v/v)
    E3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    100 μl of Magnesium chloride (2 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of water (100 v/v)
    E4
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    100 μl of Lithium sulfate (2 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of water (100 v/v)
    E5
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 12 w/v -
    NA
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    702 μl of water (100 v/v)
    E6
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    pH 4.6
    20 μl of Sodium chloride (5 M)
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    640 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 6,000 12 w/v -
    pH 4.6
    47 μl of Sodium acetate pH 4 (1 M)
    52 μl of Sodium acetate pH 5 (1 M)
    50 μl of Magnesium chloride (2 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    610 μl of water (100 v/v)
    E8
    • Magnesium chloride 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 6,000 12 w/v -
    pH 6.5
    50 μl of Magnesium chloride (2 M)
    100 μl of ADA pH 6.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    610 μl of water (100 v/v)
    F1
    • Ammonium di-hydrogen phosphate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 12 w/v -
    pH 8.5
    40 μl of Ammonium di-hydrogen phosphate (2.5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    620 μl of water (100 v/v)
    F2
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 2 w/v -
    NA
    500 μl of Lithium sulfate (2 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    F3
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    66 μl of Sodium acetate (3 M)
    533 μl of water (100 v/v)
    F4
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Zinc acetate (1 M)
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    400 μl of water (100 v/v)
    F5
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    400 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    0 μl of tris chloride BCC pH 6 (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    F7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    742 μl of water (100 v/v)
    F8
    • Lithium sulfate 0.5 M -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    250 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    550 μl of water (100 v/v)