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Supplemental Information

Screen Description

MemStart

Created 2009-11-16.

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MemStart

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Screen Structure

MemStart

Well Count 48
Number of distinct ingredients 25
Internal diversity (score mean dist) 0.923

Screen Composition

2025-08-16T09:05:42.199382 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemStart
Buffer 53
Organic (non-volatile) 8
Polymer 30
Salt 65

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemStart Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
571.4 μl of Ammonium sulfate (3.5 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
328.6 μl of water (100 v/v)
A2
  • ADA 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
285.7 μl of Ammonium sulfate (3.5 M)
614.3 μl of water (100 v/v)
A3
  • Ammonium sulfate 2 M -
NA
571.4 μl of Ammonium sulfate (3.5 M)
428.6 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium sulfate 1 M -
pH 4.6
400.0 μl of Magnesium sulfate (2.5 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
500.0 μl of water (100 v/v)
A7
  • tri-Sodium citrate 0.1 M pH 5.6
  • Magnesium sulfate 1 M -
pH 5.6
400.0 μl of Magnesium sulfate (2.5 M)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
A8
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Magnesium sulfate 1 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
400.0 μl of Magnesium sulfate (2.5 M)
450.0 μl of water (100 v/v)
B1
  • Ammonium di-hydrogen phosphate 0.1 M pH 6.5
pH 6.5
40.0 μl of Ammonium di-hydrogen phosphate pH 6.5 (2.5 M)
960.0 μl of water (100 v/v)
B2
  • Ammonium sulfite 0.1 M -
  • Potassium di-hydrogen phosphate 0.5 M pH 7.5
  • Sodium di-hydrogen phosphate 0.5 M pH 7.5
NA
50.0 μl of Ammonium sulfite (2 M)
250.0 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
23.9 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
76.1 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
600.0 μl of water (100 v/v)
B3
  • Lithium sulfate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 4.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
50.0 μl of Lithium sulfate (2 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
450.0 μl of water (100 v/v)
B4
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
B5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
B6
  • Sodium formate 2 M -
NA
285.7 μl of Sodium formate (7 M)
714.3 μl of water (100 v/v)
B7
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
B8
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
466.7 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
C1
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 1 M -
pH 7.5
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
666.7 μl of Potassium Sodium tartrate (1.5 M)
233.3 μl of water (100 v/v)
C3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
  • Ammonium sulfate 2 M -
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
308.6 μl of water (100 v/v)
C4
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
pH 4.6
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
550.0 μl of water (100 v/v)
C5
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 30 v/v -
pH 5.6
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
C6
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 400 30 v/v -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
587.5 μl of water (100 v/v)
C7
  • Lithium sulfate 0.3 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
150.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
C8
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
D1
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
571.4 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
D3
  • Zinc acetate 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
100.0 μl of Zinc acetate (1 M)
560.0 μl of water (100 v/v)
D4
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
602.9 μl of water (100 v/v)
D5
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
660.0 μl of water (100 v/v)
D6
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
647.5 μl of water (100 v/v)
D7
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 12 w/v -
pH 5.6
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
677.5 μl of water (100 v/v)
D8
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 12 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
610.0 μl of water (100 v/v)
E1
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
E2
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 12 w/v -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
-0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
631.4 μl of water (100 v/v)
E3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
E4
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
E5
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 12 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
702.9 μl of water (100 v/v)
E6
  • Sodium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
20.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
E7
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 6,000 12 w/v -
pH 4.6
50.0 μl of Magnesium chloride (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
610.0 μl of water (100 v/v)
E8
  • Magnesium chloride 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 6,000 12 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
610.0 μl of water (100 v/v)
F1
  • Ammonium di-hydrogen phosphate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 12 w/v -
pH 8.5
40.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
620.0 μl of water (100 v/v)
F2
  • Lithium sulfate 1 M -
  • Polyethylene glycol 8,000 2 w/v -
NA
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
460.0 μl of water (100 v/v)
F3
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
533.3 μl of water (100 v/v)
F4
  • Zinc acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Zinc acetate (1 M)
400.0 μl of water (100 v/v)
F5
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
F6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
0.0 μl of tris chloride BCC pH 6 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
F7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
742.9 μl of water (100 v/v)
F8
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
250.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
550.0 μl of water (100 v/v)