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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
MemStart
Barcodes
MemStart
Screen Structure
MemStart
Well Count | 48 |
---|---|
Number of distinct ingredients | 25 |
Internal diversity (score mean dist) | 0.923 |
Screen Composition
ingredient type | MemStart |
---|---|
Buffer | 53 |
Organic (non-volatile) | 8 |
Polymer | 30 |
Salt | 65 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v) |
A2 |
| pH 6.5 | 100 μl of ADA pH 6.5 (1 M) 285 μl of Ammonium sulfate (3.5 M) 614 μl of water (100 v/v) |
A3 |
| NA | 571 μl of Ammonium sulfate (3.5 M) 428 μl of water (100 v/v) |
A4 |
| pH 8.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 328 μl of water (100 v/v) |
A5 |
| pH 7.5 | 750 μl of Lithium sulfate (2 M) 100 μl of HEPES pH 7.5 (1 M) 150 μl of water (100 v/v) |
A6 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 400 μl of Magnesium sulfate (2.5 M) 500 μl of water (100 v/v) |
A7 |
| pH 5.6 | 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 400 μl of Magnesium sulfate (2.5 M) 537 μl of water (100 v/v) |
A8 |
| pH 6.5 | 50 μl of Lithium sulfate (2 M) 100 μl of ADA pH 6.5 (1 M) 400 μl of Magnesium sulfate (2.5 M) 450 μl of water (100 v/v) |
B1 |
| pH 6.5 | 40 μl of Ammonium di-hydrogen phosphate pH 6.5 (2.5 M) 960 μl of water (100 v/v) |
B2 |
| NA | 50 μl of Ammonium sulfite (2 M) 23 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M) 250 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M) 76 μl of Sodium di-hydrogen phosphate pH 8 (5 M) 600 μl of water (100 v/v) |
B3 |
| pH 4.6 | 50 μl of Lithium sulfate (2 M) 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 450 μl of water (100 v/v) |
B4 |
| pH 5.6 | 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 537 μl of water (100 v/v) |
B5 |
| pH 8.5 | 800 μl of Ammonium di-hydrogen phosphate (2.5 M) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v) |
B6 |
| NA | 285 μl of Sodium formate (7 M) 714 μl of water (100 v/v) |
B7 |
| NA | 571 μl of Sodium formate (7 M) 428 μl of water (100 v/v) |
B8 |
| pH 6.5 | 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 466 μl of Sodium acetate (3 M) 333 μl of water (100 v/v) |
C1 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 100 μl of HEPES pH 7.5 (1 M) 875 μl of tri-Sodium citrate (1.6 M) 25 μl of water (100 v/v) |
C2 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 666 μl of Potassium Sodium tartrate (1.5 M) 100 μl of HEPES pH 7.5 (1 M) 233 μl of water (100 v/v) |
C3 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 100 μl of HEPES pH 7.5 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 571 μl of Ammonium sulfate (3.5 M) 308 μl of water (100 v/v) |
C4 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 50 μl of Magnesium chloride (2 M) 300 μl of Polyethylene glycol 400 (100 v/v) 550 μl of water (100 v/v) |
C5 |
| pH 5.6 | 20 μl of Sodium chloride (5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 300 μl of Polyethylene glycol 400 (100 v/v) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 617 μl of water (100 v/v) |
C6 |
| pH 5.6 | 50 μl of Lithium sulfate (2 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 300 μl of Polyethylene glycol 400 (100 v/v) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 587 μl of water (100 v/v) |
C7 |
| pH 6.5 | 150 μl of Lithium sulfate (2 M) 100 μl of ADA pH 6.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 450 μl of water (100 v/v) |
C8 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 50 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 550 μl of water (100 v/v) |
D1 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 28 μl of Ammonium sulfate (3.5 M) 571 μl of water (100 v/v) |
D2 |
| pH 8.5 | 125 μl of tri-Sodium citrate (1.6 M) 300 μl of Polyethylene glycol 400 (100 v/v) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 475 μl of water (100 v/v) |
D3 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Zinc acetate (1 M) 560 μl of water (100 v/v) |
D4 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 602 μl of water (100 v/v) |
D5 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 660 μl of water (100 v/v) |
D6 |
| pH 5.6 | 50 μl of Lithium sulfate (2 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 647 μl of water (100 v/v) |
D7 |
| pH 5.6 | 20 μl of Sodium chloride (5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 677 μl of water (100 v/v) |
D8 |
| pH 6.5 | 50 μl of Lithium sulfate (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of ADA pH 6.5 (1 M) 610 μl of water (100 v/v) |
E1 |
| pH 7.5 | 20 μl of Sodium chloride (5 M) 0 μl of HEPES pH 5 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 640 μl of water (100 v/v) |
E2 |
| pH 7.5 | 0 μl of HEPES pH 5 (1 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 28 μl of Ammonium sulfate (3.5 M) 631 μl of water (100 v/v) |
E3 |
| pH 8.5 | 100 μl of Magnesium chloride (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 560 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100 μl of Lithium sulfate (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 560 μl of water (100 v/v) |
E5 |
| NA | 240 μl of Polyethylene glycol 4,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 702 μl of water (100 v/v) |
E6 |
| pH 4.6 | 20 μl of Sodium chloride (5 M) 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 640 μl of water (100 v/v) |
E7 |
| pH 4.6 | 47 μl of Sodium acetate pH 4 (1 M) 52 μl of Sodium acetate pH 5 (1 M) 50 μl of Magnesium chloride (2 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 610 μl of water (100 v/v) |
E8 |
| pH 6.5 | 50 μl of Magnesium chloride (2 M) 100 μl of ADA pH 6.5 (1 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 610 μl of water (100 v/v) |
F1 |
| pH 8.5 | 40 μl of Ammonium di-hydrogen phosphate (2.5 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 620 μl of water (100 v/v) |
F2 |
| NA | 500 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v) |
F3 |
| pH 6.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 66 μl of Sodium acetate (3 M) 533 μl of water (100 v/v) |
F4 |
| pH 6.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Zinc acetate (1 M) 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 400 μl of water (100 v/v) |
F5 |
| pH 6.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Calcium acetate (1 M) 151 μl of MES pH 7.1 (0.5 M) 48 μl of MES pH 5.2 (0.5 M) 400 μl of water (100 v/v) |
F6 |
| pH 8.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 0 μl of tris chloride BCC pH 6 (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 700 μl of water (100 v/v) |
F7 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 742 μl of water (100 v/v) |
F8 |
| NA | 250 μl of Lithium sulfate (2 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 550 μl of water (100 v/v) |