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Supplemental Information

Screen Description

MemSys

Created 2009-11-16.

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MemSys

    Screen Structure

    MemSys

    Well Count 48
    Number of distinct ingredients 16
    Internal diversity (score mean dist) 0.777

    Screen Composition

    2025-06-15T02:22:17.371901 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemSys
    Buffer 52
    Organic (non-volatile) 20
    Polymer 40
    Salt 78

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemSys Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 5.5
    • Ammonium sulfate 2.5 M -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    714 μl of Ammonium sulfate (3.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    223 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 3.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 3.5
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    13 μl of tri-Sodium citrate pH 3 (1.6 M)
    49 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    567 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 4.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    71 μl of Sodium acetate pH 4 (1 M)
    28 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    530 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    617 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 5.5
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    567 μl of water (100 v/v)
    A6
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    50 μl of Magnesium chloride (2 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    567 μl of water (100 v/v)
    A7
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 2.5 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    714 μl of Ammonium sulfate (3.5 M)
    85 μl of water (100 v/v)
    A8
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    500 μl of water (100 v/v)
    B1
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    20 μl of Sodium chloride (5 M)
    480 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    20 μl of Sodium chloride (5 M)
    430 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    430 μl of water (100 v/v)
    B4
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 30 v/v -
    pH 7
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of MOPS pH 7.9 (1 M)
    67 μl of MOPS pH 6.5 (1 M)
    600 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2.5 M -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    714 μl of Ammonium sulfate (3.5 M)
    185 μl of water (100 v/v)
    B6
    • MOPS 0.1 M pH 7
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 7
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of MOPS pH 7.9 (1 M)
    20 μl of Sodium chloride (5 M)
    67 μl of MOPS pH 6.5 (1 M)
    580 μl of water (100 v/v)
    B7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    51 μl of HEPES pH 6.6 (1 M)
    600 μl of water (100 v/v)
    B8
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    51 μl of HEPES pH 6.6 (1 M)
    20 μl of Sodium chloride (5 M)
    580 μl of water (100 v/v)
    C1
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    50 μl of Lithium sulfate (2 M)
    48 μl of HEPES pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    51 μl of HEPES pH 6.6 (1 M)
    20 μl of Sodium chloride (5 M)
    530 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0 M -
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    51 μl of HEPES pH 6.6 (1 M)
    0 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    580 μl of water (100 v/v)
    C3
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1.5 M -
    pH 8.5
    750 μl of Lithium sulfate (2 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    150 μl of water (100 v/v)
    C4
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    580 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    530 μl of water (100 v/v)
    C6
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    530 μl of water (100 v/v)
    C7
    • CAPSO 0.1 M pH 9.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 9.5
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    117 μl of CAPSO pH 8.9 (0.5 M)
    20 μl of Sodium chloride (5 M)
    82 μl of CAPSO pH 10 (0.5 M)
    430 μl of water (100 v/v)
    C8
    • CAPSO 0.1 M pH 9.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 30 v/v -
    pH 9.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    117 μl of CAPSO pH 8.9 (0.5 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    82 μl of CAPSO pH 10 (0.5 M)
    430 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 5.5
    • di-Sodium hydrogen phosphate 1.5 M -
    pH 5.5
    375 μl of di-Sodium hydrogen phosphate (4 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    562 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M pH 3.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 3.5
    13 μl of tri-Sodium citrate pH 3 (1.6 M)
    50 μl of Magnesium chloride (2 M)
    49 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    627 μl of water (100 v/v)
    D3
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 4.5
    50 μl of Lithium sulfate (2 M)
    71 μl of Sodium acetate pH 4 (1 M)
    28 μl of Sodium acetate pH 5.5 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    590 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    677 μl of water (100 v/v)
    D5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 5.5
    50 μl of Lithium sulfate (2 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    627 μl of water (100 v/v)
    D6
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    50 μl of Magnesium chloride (2 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    627 μl of water (100 v/v)
    D7
    • MES 0.1 M pH 6.5
    • Sodium di-hydrogen phosphate 1.5 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    300 μl of Sodium di-hydrogen phosphate (5 M)
    500 μl of water (100 v/v)
    D8
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    560 μl of water (100 v/v)
    E1
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    540 μl of water (100 v/v)
    E2
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    50 μl of Lithium sulfate (2 M)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    490 μl of water (100 v/v)
    E3
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    490 μl of water (100 v/v)
    E4
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 4,000 12 w/v -
    pH 7
    33 μl of MOPS pH 7.9 (1 M)
    67 μl of MOPS pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    660 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 1.5 M -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    750 μl of Potassium di-hydrogen phosphate (2 M)
    150 μl of water (100 v/v)
    E6
    • MOPS 0.1 M pH 7
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 7
    33 μl of MOPS pH 7.9 (1 M)
    20 μl of Sodium chloride (5 M)
    67 μl of MOPS pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    640 μl of water (100 v/v)
    E7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    660 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    640 μl of water (100 v/v)
    F1
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    50 μl of Lithium sulfate (2 M)
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    590 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 7.5
    48 μl of HEPES pH 8.5 (1 M)
    51 μl of HEPES pH 6.6 (1 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    590 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium di-hydrogen phosphate 1.5 M -
    pH 8.5
    750 μl of Potassium di-hydrogen phosphate (2 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    150 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    78 μl of tris chloride BCC pH 9 (1 M)
    640 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    50 μl of Lithium sulfate (2 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    78 μl of tris chloride BCC pH 9 (1 M)
    590 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.5
    50 μl of Magnesium chloride (2 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    78 μl of tris chloride BCC pH 9 (1 M)
    590 μl of water (100 v/v)
    F7
    • CAPSO 0.1 M pH 9.5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 9.5
    50 μl of Lithium sulfate (2 M)
    117 μl of CAPSO pH 8.9 (0.5 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    82 μl of CAPSO pH 10 (0.5 M)
    490 μl of water (100 v/v)
    F8
    • CAPSO 0.1 M pH 9.5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 4,000 12 w/v -
    pH 9.5
    117 μl of CAPSO pH 8.9 (0.5 M)
    50 μl of Magnesium chloride (2 M)
    20 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    82 μl of CAPSO pH 10 (0.5 M)
    490 μl of water (100 v/v)