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Supplemental Information

Screen Description

NR-LBD

Created 2009-11-16.

Export

Barcodes

NR-LBD

No barcodes found.

Screen Structure

NR-LBD

Well Count 96
Number of distinct ingredients 21
Internal diversity (score mean dist) 0.887

Screen Composition

2025-08-16T16:27:05.706161 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type NR-LBD
Buffer 147
Organic (non-volatile) 3
Polymer 51
Salt 119

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
NR-LBD Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
A2
  • Sodium chloride 0.9 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 8,000 6 w/v -
pH 8
120.0 μl of Polyethylene glycol 8,000 (50 w/v)
180.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
600.0 μl of water (100 v/v)
A3
  • Sodium chloride 0.5 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 19 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
A4
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 13 w/v -
pH 8
200.0 μl of Ammonium acetate (1 M)
260.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
440.0 μl of water (100 v/v)
A5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 8,000 9 w/v -
pH 8
180.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
720.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 24 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
380.0 μl of water (100 v/v)
A7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 12 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
660.0 μl of water (100 v/v)
A8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 18 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 6,000 (50 w/v)
540.0 μl of water (100 v/v)
A9
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 8,000 7 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
140.0 μl of Polyethylene glycol 8,000 (50 w/v)
760.0 μl of water (100 v/v)
A10
  • Ammonium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 18 w/v -
pH 7.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
440.0 μl of water (100 v/v)
A11
  • Ammonium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 8 w/v -
pH 7.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
640.0 μl of water (100 v/v)
A12
  • Sodium chloride 0.3 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 27 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
540.0 μl of Polyethylene glycol 4,000 (50 w/v)
60.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
B1
  • Sodium chloride 0.3 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
60.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
B2
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 23 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
460.0 μl of Polyethylene glycol 4,000 (50 w/v)
440.0 μl of water (100 v/v)
B3
  • Ammonium acetate 1.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 14 w/v -
pH 8.5
400.0 μl of Ammonium acetate (3 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
B4
  • Sodium chloride 0.4 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 19 w/v -
pH 8
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
440.0 μl of water (100 v/v)
B5
  • Sodium chloride 0.6 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 7 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
140.0 μl of Polyethylene glycol 4,000 (50 w/v)
120.0 μl of Sodium chloride (5 M)
640.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 15 w/v -
pH 8
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
600.0 μl of water (100 v/v)
B7
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 8,000 8 w/v -
pH 8
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
740.0 μl of water (100 v/v)
B8
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 22 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
460.0 μl of water (100 v/v)
B9
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 10 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
B10
  • Sodium chloride 0.5 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 6,000 14 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
280.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
B11
  • Sodium chloride 0.4 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
B12
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 17 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
560.0 μl of water (100 v/v)
C1
  • PIPES 0.1 M pH 7
  • Sodium acetate 1 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
333.3 μl of Sodium acetate (3 M)
566.7 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
812.5 μl of tri-Sodium citrate (1.6 M)
87.5 μl of water (100 v/v)
C3
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium formate 3 M -
pH 8.5
66.7 μl of Ammonium acetate (3 M)
428.6 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
404.8 μl of water (100 v/v)
C4
  • Ammonium acetate 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 1 M -
pH 8
33.3 μl of Ammonium acetate (3 M)
625.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
241.7 μl of water (100 v/v)
C5
  • Ammonium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1.6 M -
pH 7.5
33.3 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
533.3 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
C6
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.2 M -
pH 7.5
66.7 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of tri-Sodium citrate (1.6 M)
83.3 μl of water (100 v/v)
C7
  • Sodium chloride 0.4 M -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Sodium chloride (5 M)
812.5 μl of tri-Sodium citrate (1.6 M)
7.5 μl of water (100 v/v)
C8
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1.6 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
533.3 μl of Sodium acetate (3 M)
366.7 μl of water (100 v/v)
C9
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8
  • Sodium acetate 0.9 M -
pH 8
300.0 μl of Sodium acetate (3 M)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
C10
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
33.3 μl of Ammonium acetate (3 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
241.7 μl of water (100 v/v)
C11
  • Sodium chloride 0.3 M -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Sodium acetate (3 M)
60.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
C12
  • Sodium chloride 0.3 M -
  • PIPES 0.1 M pH 7
  • Sodium formate 2.6 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
60.0 μl of Sodium chloride (5 M)
371.4 μl of Sodium formate (7 M)
468.6 μl of water (100 v/v)
D1
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
466.7 μl of Sodium acetate (3 M)
433.3 μl of water (100 v/v)
D2
  • PIPES 0.1 M pH 7
  • tri-Sodium citrate 1.5 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
500.0 μl of tri-Sodium citrate (3 M)
400.0 μl of water (100 v/v)
D3
  • Sodium chloride 0.8 M -
  • PIPES 0.1 M pH 7
  • tri-Sodium citrate 1.2 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
160.0 μl of Sodium chloride (5 M)
400.0 μl of tri-Sodium citrate (3 M)
340.0 μl of water (100 v/v)
D4
  • Ammonium acetate 0.1 M -
  • PIPES 0.1 M pH 7
  • Sodium formate 2.5 M -
pH 7
33.3 μl of Ammonium acetate (3 M)
100.0 μl of PIPES pH 7 (1 M)
357.1 μl of Sodium formate (7 M)
509.5 μl of water (100 v/v)
D5
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium formate 2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
20.0 μl of Sodium chloride (5 M)
285.7 μl of Sodium formate (7 M)
594.3 μl of water (100 v/v)
D6
  • Sodium chloride 0.4 M -
  • BIS-TRIS 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Sodium chloride (5 M)
333.3 μl of tri-Sodium citrate (3 M)
486.7 μl of water (100 v/v)
D7
  • Ammonium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1 M -
pH 7.5
33.3 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
333.3 μl of tri-Sodium citrate (3 M)
533.3 μl of water (100 v/v)
D8
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 1.3 M -
pH 8.5
433.3 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
466.7 μl of water (100 v/v)
D9
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
266.7 μl of Sodium acetate (3 M)
633.3 μl of water (100 v/v)
D10
  • tris chloride BCC 0.1 M pH 8
  • Sodium formate 3.5 M -
pH 8
500.0 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
D11
  • Ammonium acetate 0.2 M -
  • PIPES 0.1 M pH 7
  • Sodium formate 2.7 M -
pH 7
66.7 μl of Ammonium acetate (3 M)
100.0 μl of PIPES pH 7 (1 M)
385.7 μl of Sodium formate (7 M)
447.6 μl of water (100 v/v)
D12
  • Sodium chloride 0.5 M -
  • HEPES 0.1 M pH 7.5
  • Sodium formate 2.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Sodium chloride (5 M)
314.3 μl of Sodium formate (7 M)
485.7 μl of water (100 v/v)
E1
  • Ammonium acetate 0.1 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 400 21 v/v -
pH 7
33.3 μl of Ammonium acetate (3 M)
100.0 μl of PIPES pH 7 (1 M)
210.0 μl of Polyethylene glycol 400 (100 v/v)
656.7 μl of water (100 v/v)
E2
  • Sodium chloride 0.3 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 25 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
60.0 μl of Sodium chloride (5 M)
590.0 μl of water (100 v/v)
E3
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 400 28 v/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
562.9 μl of water (100 v/v)
E4
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
E5
  • Sodium chloride 0.1 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
440.0 μl of water (100 v/v)
E6
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 19 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
380.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
480.0 μl of water (100 v/v)
E7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
460.0 μl of water (100 v/v)
E8
  • Ammonium sulfate 0.3 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 11 w/v -
pH 7
85.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of PIPES pH 7 (1 M)
220.0 μl of Polyethylene glycol 4,000 (50 w/v)
594.3 μl of water (100 v/v)
E9
  • Ammonium sulfate 0.5 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 12 w/v -
pH 7
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of PIPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
517.1 μl of water (100 v/v)
E10
  • Sodium thiocyanate 0.4 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 13 w/v -
pH 8.5
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
590.0 μl of water (100 v/v)
E11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 15 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
E12
  • Magnesium chloride 0.2 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 4,000 22 w/v -
pH 7
100.0 μl of Magnesium chloride (2 M)
100.0 μl of PIPES pH 7 (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
360.0 μl of water (100 v/v)
F1
  • Sodium thiocyanate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 23 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
460.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
415.0 μl of water (100 v/v)
F2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 5,000 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
460.0 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 25 w/v -
pH 8.5
85.7 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
314.3 μl of water (100 v/v)
F4
  • Ammonium acetate 0.2 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 5,000 26 w/v -
pH 7
66.7 μl of Ammonium acetate (3 M)
100.0 μl of PIPES pH 7 (1 M)
520.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
313.3 μl of water (100 v/v)
F5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 7 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
140.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
F6
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 11 w/v -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
220.0 μl of Polyethylene glycol 6,000 (50 w/v)
622.9 μl of water (100 v/v)
F7
  • Sodium thiocyanate 0.4 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 6,000 17 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
340.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium thiocyanate (8 M)
510.0 μl of water (100 v/v)
F8
  • Sodium thiocyanate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 8,000 10 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
675.0 μl of water (100 v/v)
F9
  • Magnesium chloride 0.2 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 8,000 12 w/v -
pH 7
100.0 μl of Magnesium chloride (2 M)
100.0 μl of PIPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
560.0 μl of water (100 v/v)
F10
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 8,000 14 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
280.0 μl of Polyethylene glycol 8,000 (50 w/v)
562.9 μl of water (100 v/v)
F11
  • Sodium thiocyanate 0.1 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 8,000 15 w/v -
pH 7
100.0 μl of PIPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
12.5 μl of Sodium thiocyanate (8 M)
587.5 μl of water (100 v/v)
F12
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 18 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
490.0 μl of water (100 v/v)
G1
  • Sodium thiocyanate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 10,000 9 w/v -
pH 8.5
180.0 μl of Polyethylene glycol 10,000 (50 w/v)
12.5 μl of Sodium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
707.5 μl of water (100 v/v)
G2
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 12 w/v -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 10,000 (50 w/v)
631.4 μl of water (100 v/v)
G3
  • Ammonium acetate 0.2 M -
  • PIPES 0.1 M pH 7
  • Polyethylene glycol 10,000 16 w/v -
pH 7
66.7 μl of Ammonium acetate (3 M)
100.0 μl of PIPES pH 7 (1 M)
320.0 μl of Polyethylene glycol 10,000 (50 w/v)
513.3 μl of water (100 v/v)
G4
  • Sodium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 1 M -
pH 8.5
333.3 μl of Sodium acetate (3 M)
25.0 μl of Sodium thiocyanate (8 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
541.7 μl of water (100 v/v)
G5
  • Ammonium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 1.1 M -
pH 8
28.6 μl of Ammonium sulfate (3.5 M)
366.7 μl of tri-Sodium citrate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
504.8 μl of water (100 v/v)
G6
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Sodium formate 2.7 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
385.7 μl of Sodium formate (7 M)
414.3 μl of water (100 v/v)
G7
  • Ammonium acetate 0.3 M -
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 0.8 M -
pH 7.5
100.0 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of di-Sodium tartrate (2 M)
400.0 μl of water (100 v/v)
G8
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • di-Sodium tartrate 0.8 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
400.0 μl of di-Sodium tartrate (2 M)
460.0 μl of water (100 v/v)
G9
  • Sodium thiocyanate 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium tartrate 0.9 M -
pH 8
12.5 μl of Sodium thiocyanate (8 M)
450.0 μl of di-Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
437.5 μl of water (100 v/v)
G10
  • Ammonium sulfate 0.5 M -
  • PIPES 0.1 M pH 7
  • di-Sodium tartrate 0.9 M -
pH 7
142.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of PIPES pH 7 (1 M)
450.0 μl of di-Sodium tartrate (2 M)
307.1 μl of water (100 v/v)
G11
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium tartrate 1.1 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
550.0 μl of di-Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
292.9 μl of water (100 v/v)
G12
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 1.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
550.0 μl of di-Sodium tartrate (2 M)
300.0 μl of water (100 v/v)
H1
  • Sodium thiocyanate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Lithium sulfate 0.7 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
350.0 μl of Lithium sulfate (2 M)
25.0 μl of Sodium thiocyanate (8 M)
525.0 μl of water (100 v/v)
H2
  • Ammonium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.8 M -
pH 7.5
33.3 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Lithium sulfate (2 M)
466.7 μl of water (100 v/v)
H3
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1 M -
pH 8.5
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
H4
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
550.0 μl of Lithium sulfate (2 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of water (100 v/v)
H5
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
650.0 μl of Lithium sulfate (2 M)
250.0 μl of water (100 v/v)
H6
  • BIS-TRIS 0.1 M pH 6.5
  • Lithium sulfate 1.4 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
700.0 μl of Lithium sulfate (2 M)
200.0 μl of water (100 v/v)
H7
  • Sodium chloride 0.2 M -
  • PIPES 0.1 M pH 7
  • Ammonium sulfate 1.3 M -
pH 7
371.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of PIPES pH 7 (1 M)
40.0 μl of Sodium chloride (5 M)
488.6 μl of water (100 v/v)
H8
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.4 M -
pH 7.5
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of water (100 v/v)
H9
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.5 M -
pH 8.5
428.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of Magnesium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
421.4 μl of water (100 v/v)
H10
  • Sodium thiocyanate 0.2 M -
  • PIPES 0.1 M pH 7
  • Ammonium sulfate 1.6 M -
pH 7
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of PIPES pH 7 (1 M)
25.0 μl of Sodium thiocyanate (8 M)
417.9 μl of water (100 v/v)
H11
  • Sodium chloride 0.3 M -
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 1.7 M -
pH 8
485.7 μl of Ammonium sulfate (3.5 M)
60.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
354.3 μl of water (100 v/v)
H12
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 2 M -
pH 8
571.4 μl of Ammonium sulfate (3.5 M)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
288.6 μl of water (100 v/v)