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Supplemental Information

Screen Description

NR-LBD

Created 2009-11-16.

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NR-LBD

    Screen Structure

    NR-LBD

    Well Count 96
    Number of distinct ingredients 21
    Internal diversity (score mean dist) 0.887

    Screen Composition

    2025-06-14T13:26:06.245672 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type NR-LBD
    Buffer 147
    Organic (non-volatile) 3
    Polymer 51
    Salt 119

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    NR-LBD Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    A2
    • Sodium chloride 0.9 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 6 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    120 μl of Polyethylene glycol 8,000 (50 w/v)
    180 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    A3
    • Sodium chloride 0.5 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 19 w/v -
    pH 6.5
    100 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    420 μl of water (100 v/v)
    A4
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 13 w/v -
    pH 8
    200 μl of Ammonium acetate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    260 μl of Polyethylene glycol 6,000 (50 w/v)
    440 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 9 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    720 μl of water (100 v/v)
    A6
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 24 w/v -
    pH 7.5
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    380 μl of water (100 v/v)
    A7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 12 w/v -
    pH 7.5
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    660 μl of water (100 v/v)
    A8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 18 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    360 μl of Polyethylene glycol 6,000 (50 w/v)
    540 μl of water (100 v/v)
    A9
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 8,000 7 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    140 μl of Polyethylene glycol 8,000 (50 w/v)
    760 μl of water (100 v/v)
    A10
    • Ammonium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 18 w/v -
    pH 7.5
    100 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    360 μl of Polyethylene glycol 4,000 (50 w/v)
    440 μl of water (100 v/v)
    A11
    • Ammonium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 7.5
    100 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    640 μl of water (100 v/v)
    A12
    • Sodium chloride 0.3 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 27 w/v -
    pH 6.5
    60 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    540 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    B1
    • Sodium chloride 0.3 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 6.5
    60 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    520 μl of water (100 v/v)
    B2
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 23 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    460 μl of Polyethylene glycol 4,000 (50 w/v)
    440 μl of water (100 v/v)
    B3
    • Ammonium acetate 1.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 14 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ammonium acetate (3 M)
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    220 μl of water (100 v/v)
    B4
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 19 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Sodium chloride (5 M)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    440 μl of water (100 v/v)
    B5
    • Sodium chloride 0.6 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 7 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    120 μl of Sodium chloride (5 M)
    140 μl of Polyethylene glycol 4,000 (50 w/v)
    640 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 15 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    B7
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 8 w/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    740 μl of water (100 v/v)
    B8
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 22 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    460 μl of water (100 v/v)
    B9
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 8.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    660 μl of water (100 v/v)
    B10
    • Sodium chloride 0.5 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 6,000 14 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    100 μl of Sodium chloride (5 M)
    280 μl of Polyethylene glycol 6,000 (50 w/v)
    520 μl of water (100 v/v)
    B11
    • Sodium chloride 0.4 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    80 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    420 μl of water (100 v/v)
    B12
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 17 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    560 μl of water (100 v/v)
    C1
    • PIPES 0.1 M pH 7
    • Sodium acetate 1 M -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    333 μl of Sodium acetate (3 M)
    566 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.3 M -
    pH 7.5
    812 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    87 μl of water (100 v/v)
    C3
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 3 M -
    pH 8.5
    428 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of Ammonium acetate (3 M)
    404 μl of water (100 v/v)
    C4
    • Ammonium acetate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 1 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    33 μl of Ammonium acetate (3 M)
    241 μl of water (100 v/v)
    C5
    • Ammonium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1.6 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Sodium acetate (3 M)
    33 μl of Ammonium acetate (3 M)
    333 μl of water (100 v/v)
    C6
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.2 M -
    pH 7.5
    750 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Ammonium acetate (3 M)
    83 μl of water (100 v/v)
    C7
    • Sodium chloride 0.4 M -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.3 M -
    pH 7.5
    80 μl of Sodium chloride (5 M)
    812 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    7 μl of water (100 v/v)
    C8
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1.6 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Sodium acetate (3 M)
    366 μl of water (100 v/v)
    C9
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8
    • Sodium acetate 0.9 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Sodium chloride (5 M)
    300 μl of Sodium acetate (3 M)
    500 μl of water (100 v/v)
    C10
    • Ammonium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    pH 6.5
    625 μl of tri-Sodium citrate (1.6 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    33 μl of Ammonium acetate (3 M)
    241 μl of water (100 v/v)
    C11
    • Sodium chloride 0.3 M -
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1.5 M -
    pH 7.5
    60 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Sodium acetate (3 M)
    340 μl of water (100 v/v)
    C12
    • Sodium chloride 0.3 M -
    • PIPES 0.1 M pH 7
    • Sodium formate 2.6 M -
    pH 7
    371 μl of Sodium formate (7 M)
    100 μl of PIPES pH 7 (1 M)
    60 μl of Sodium chloride (5 M)
    468 μl of water (100 v/v)
    D1
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    466 μl of Sodium acetate (3 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    433 μl of water (100 v/v)
    D2
    • PIPES 0.1 M pH 7
    • tri-Sodium citrate 1.5 M -
    pH 7
    500 μl of tri-Sodium citrate (3 M)
    100 μl of PIPES pH 7 (1 M)
    400 μl of water (100 v/v)
    D3
    • Sodium chloride 0.8 M -
    • PIPES 0.1 M pH 7
    • tri-Sodium citrate 1.2 M -
    pH 7
    400 μl of tri-Sodium citrate (3 M)
    100 μl of PIPES pH 7 (1 M)
    160 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    D4
    • Ammonium acetate 0.1 M -
    • PIPES 0.1 M pH 7
    • Sodium formate 2.5 M -
    pH 7
    357 μl of Sodium formate (7 M)
    100 μl of PIPES pH 7 (1 M)
    33 μl of Ammonium acetate (3 M)
    509 μl of water (100 v/v)
    D5
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium formate 2 M -
    pH 6.5
    285 μl of Sodium formate (7 M)
    20 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    594 μl of water (100 v/v)
    D6
    • Sodium chloride 0.4 M -
    • BIS-TRIS 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    pH 6.5
    333 μl of tri-Sodium citrate (3 M)
    80 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    486 μl of water (100 v/v)
    D7
    • Ammonium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1 M -
    pH 7.5
    333 μl of tri-Sodium citrate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    33 μl of Ammonium acetate (3 M)
    533 μl of water (100 v/v)
    D8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 1.3 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    433 μl of Sodium acetate (3 M)
    466 μl of water (100 v/v)
    D9
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.8 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    266 μl of Sodium acetate (3 M)
    633 μl of water (100 v/v)
    D10
    • tris chloride BCC 0.1 M pH 8
    • Sodium formate 3.5 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of Sodium formate (7 M)
    400 μl of water (100 v/v)
    D11
    • Ammonium acetate 0.2 M -
    • PIPES 0.1 M pH 7
    • Sodium formate 2.7 M -
    pH 7
    385 μl of Sodium formate (7 M)
    100 μl of PIPES pH 7 (1 M)
    66 μl of Ammonium acetate (3 M)
    447 μl of water (100 v/v)
    D12
    • Sodium chloride 0.5 M -
    • HEPES 0.1 M pH 7.5
    • Sodium formate 2.2 M -
    pH 7.5
    314 μl of Sodium formate (7 M)
    100 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    485 μl of water (100 v/v)
    E1
    • Ammonium acetate 0.1 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 400 21 v/v -
    pH 7
    210 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of PIPES pH 7 (1 M)
    33 μl of Ammonium acetate (3 M)
    656 μl of water (100 v/v)
    E2
    • Sodium chloride 0.3 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 25 v/v -
    pH 7.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    60 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    590 μl of water (100 v/v)
    E3
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 28 v/v -
    pH 6.5
    280 μl of Polyethylene glycol 400 (100 v/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    562 μl of water (100 v/v)
    E4
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Magnesium chloride (2 M)
    400 μl of water (100 v/v)
    E5
    • Sodium chloride 0.1 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 22 w/v -
    pH 7
    440 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of PIPES pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    440 μl of water (100 v/v)
    E6
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 19 w/v -
    pH 6.5
    380 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    480 μl of water (100 v/v)
    E7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 22 w/v -
    pH 7.5
    440 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    E8
    • Ammonium sulfate 0.3 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 11 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    85 μl of Ammonium sulfate (3.5 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    594 μl of water (100 v/v)
    E9
    • Ammonium sulfate 0.5 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 12 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    142 μl of Ammonium sulfate (3.5 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    517 μl of water (100 v/v)
    E10
    • Sodium thiocyanate 0.4 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 13 w/v -
    pH 8.5
    50 μl of Sodium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    260 μl of Polyethylene glycol 4,000 (50 w/v)
    590 μl of water (100 v/v)
    E11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    500 μl of water (100 v/v)
    E12
    • Magnesium chloride 0.2 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 4,000 22 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    360 μl of water (100 v/v)
    F1
    • Sodium thiocyanate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 23 w/v -
    pH 6.5
    25 μl of Sodium thiocyanate (8 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of Polyethylene glycol 4,000 (50 w/v)
    415 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 22 w/v -
    pH 7.5
    440 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.3 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    85 μl of Ammonium sulfate (3.5 M)
    314 μl of water (100 v/v)
    F4
    • Ammonium acetate 0.2 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 5,000 26 w/v -
    pH 7
    520 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of PIPES pH 7 (1 M)
    66 μl of Ammonium acetate (3 M)
    313 μl of water (100 v/v)
    F5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 7 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    140 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    660 μl of water (100 v/v)
    F6
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 11 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    220 μl of Polyethylene glycol 6,000 (50 w/v)
    622 μl of water (100 v/v)
    F7
    • Sodium thiocyanate 0.4 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 6,000 17 w/v -
    pH 6.5
    50 μl of Sodium thiocyanate (8 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    340 μl of Polyethylene glycol 6,000 (50 w/v)
    510 μl of water (100 v/v)
    F8
    • Sodium thiocyanate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.5
    25 μl of Sodium thiocyanate (8 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    675 μl of water (100 v/v)
    F9
    • Magnesium chloride 0.2 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 8,000 12 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    560 μl of water (100 v/v)
    F10
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 8,000 14 w/v -
    pH 6.5
    280 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    562 μl of water (100 v/v)
    F11
    • Sodium thiocyanate 0.1 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 8,000 15 w/v -
    pH 7
    12 μl of Sodium thiocyanate (8 M)
    100 μl of PIPES pH 7 (1 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    587 μl of water (100 v/v)
    F12
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 7.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    490 μl of water (100 v/v)
    G1
    • Sodium thiocyanate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 10,000 9 w/v -
    pH 8.5
    12 μl of Sodium thiocyanate (8 M)
    180 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    707 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 12 w/v -
    pH 7.5
    240 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    631 μl of water (100 v/v)
    G3
    • Ammonium acetate 0.2 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 10,000 16 w/v -
    pH 7
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of PIPES pH 7 (1 M)
    66 μl of Ammonium acetate (3 M)
    513 μl of water (100 v/v)
    G4
    • Sodium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 1 M -
    pH 8.5
    25 μl of Sodium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Sodium acetate (3 M)
    541 μl of water (100 v/v)
    G5
    • Ammonium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 1.1 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    366 μl of tri-Sodium citrate (3 M)
    28 μl of Ammonium sulfate (3.5 M)
    504 μl of water (100 v/v)
    G6
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Sodium formate 2.7 M -
    pH 7.5
    385 μl of Sodium formate (7 M)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    414 μl of water (100 v/v)
    G7
    • Ammonium acetate 0.3 M -
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 0.8 M -
    pH 7.5
    400 μl of di-Sodium tartrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Ammonium acetate (3 M)
    400 μl of water (100 v/v)
    G8
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • di-Sodium tartrate 0.8 M -
    pH 6.5
    400 μl of di-Sodium tartrate (2 M)
    40 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of water (100 v/v)
    G9
    • Sodium thiocyanate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium tartrate 0.9 M -
    pH 8
    12 μl of Sodium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    450 μl of di-Sodium tartrate (2 M)
    437 μl of water (100 v/v)
    G10
    • Ammonium sulfate 0.5 M -
    • PIPES 0.1 M pH 7
    • di-Sodium tartrate 0.9 M -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    450 μl of di-Sodium tartrate (2 M)
    142 μl of Ammonium sulfate (3.5 M)
    307 μl of water (100 v/v)
    G11
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium tartrate 1.1 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    550 μl of di-Sodium tartrate (2 M)
    57 μl of Ammonium sulfate (3.5 M)
    292 μl of water (100 v/v)
    G12
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 1.1 M -
    pH 7.5
    550 μl of di-Sodium tartrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    H1
    • Sodium thiocyanate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Lithium sulfate 0.7 M -
    pH 6.5
    25 μl of Sodium thiocyanate (8 M)
    350 μl of Lithium sulfate (2 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    525 μl of water (100 v/v)
    H2
    • Ammonium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 0.8 M -
    pH 7.5
    400 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    33 μl of Ammonium acetate (3 M)
    466 μl of water (100 v/v)
    H3
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1 M -
    pH 8.5
    500 μl of Lithium sulfate (2 M)
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    360 μl of water (100 v/v)
    H4
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.1 M -
    pH 7.5
    550 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    H5
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.3 M -
    pH 7.5
    650 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    250 μl of water (100 v/v)
    H6
    • BIS-TRIS 0.1 M pH 6.5
    • Lithium sulfate 1.4 M -
    pH 6.5
    700 μl of Lithium sulfate (2 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of water (100 v/v)
    H7
    • Sodium chloride 0.2 M -
    • PIPES 0.1 M pH 7
    • Ammonium sulfate 1.3 M -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    371 μl of Ammonium sulfate (3.5 M)
    488 μl of water (100 v/v)
    H8
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.4 M -
    pH 7.5
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Ammonium sulfate (3.5 M)
    300 μl of water (100 v/v)
    H9
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.5 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    50 μl of Magnesium chloride (2 M)
    421 μl of water (100 v/v)
    H10
    • Sodium thiocyanate 0.2 M -
    • PIPES 0.1 M pH 7
    • Ammonium sulfate 1.6 M -
    pH 7
    25 μl of Sodium thiocyanate (8 M)
    100 μl of PIPES pH 7 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    417 μl of water (100 v/v)
    H11
    • Sodium chloride 0.3 M -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1.7 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    60 μl of Sodium chloride (5 M)
    485 μl of Ammonium sulfate (3.5 M)
    354 μl of water (100 v/v)
    H12
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 2 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    40 μl of Sodium chloride (5 M)
    571 μl of Ammonium sulfate (3.5 M)
    288 μl of water (100 v/v)