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Supplemental Information

Screen Description

ProPlex

Created 2009-11-16.

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ProPlex

    Screen Structure

    ProPlex

    Well Count 96
    Number of distinct ingredients 43
    Internal diversity (score mean dist) 0.919

    Screen Composition

    2025-06-14T14:12:48.066773 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type ProPlex
    Buffer 113
    Organic (non-volatile) 8
    Organic (volatile) 6
    Polymer 68
    Salt 98

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    ProPlex Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 350 25 v/v -
    pH 8
    250 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    A2
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 15 v/v -
    • Calcium acetate 0.1 M -
    pH 6
    117 μl of MES pH 5.2 (0.5 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    100 μl of Calcium acetate (1 M)
    550 μl of water (100 v/v)
    A3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 20 v/v -
    • Lithium chloride 0.1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Lithium chloride (10 M)
    690 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 25 v/v -
    pH 8
    250 μl of Polyethylene glycol 400 (100 v/v)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    A5
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 15 v/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    150 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    650 μl of water (100 v/v)
    A6
    • Sodium Potassium phosphate 0.1 M pH 6.5
    • Polyethylene glycol 1,000 25 w/v -
    • Sodium chloride 0.2 M -
    pH 6.5
    500 μl of Polyethylene glycol 1,000 (50 w/v)
    2 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    30 μl of Sodium Potassium phosphate pH 7 (3 M)
    40 μl of Sodium chloride (5 M)
    426 μl of water (100 v/v)
    A7
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 1,500 20 w/v -
    • Ammonium sulfate 0.1 M -
    pH 7.5
    28 μl of Ammonium sulfate (3.5 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    471 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Ammonium sulfate 0.2 M -
    pH 5.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5.5 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    642 μl of water (100 v/v)
    A9
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • Sodium chloride 0.2 M -
    pH 6
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    A10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • Potassium chloride 0.1 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    25 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    575 μl of water (100 v/v)
    A11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    400 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 5 w/v -
    • Sodium acetate 0.2 M -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    770 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 5 w/v -
    • Lithium sulfate 0.2 M -
    pH 7.5
    100 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    700 μl of water (100 v/v)
    B2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 10 w/v -
    • Calcium acetate 0.1 M -
    pH 4.5
    58 μl of Sodium acetate pH 4 (1 M)
    41 μl of Sodium acetate pH 5 (1 M)
    100 μl of Calcium acetate (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    600 μl of water (100 v/v)
    B3
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 10 w/v -
    • Sodium acetate 0.2 M -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Sodium acetate (3 M)
    670 μl of water (100 v/v)
    B4
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 10 w/v -
    • Sodium chloride 0.2 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    560 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    • Magnesium chloride 0.1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    650 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 10 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    B7
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 4,000 15 w/v -
    • Ammonium acetate 0.2 M -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    200 μl of Ammonium acetate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 4,000 15 w/v -
    • Magnesium chloride 0.1 M -
    pH 5
    50 μl of Magnesium chloride (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    587 μl of water (100 v/v)
    B9
    • Sodium cacodylate 0.1 M pH 6
    • Polyethylene glycol 4,000 15 w/v -
    pH 6
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6 (1 M)
    600 μl of water (100 v/v)
    B10
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 15 w/v -
    • Ammonium sulfate 0.15 M -
    pH 6
    117 μl of MES pH 5.2 (0.5 M)
    42 μl of Ammonium sulfate (3.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    457 μl of water (100 v/v)
    B11
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 4,000 15 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    600 μl of water (100 v/v)
    B12
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 4,000 15 w/v -
    • Magnesium chloride 0.1 M -
    pH 7
    50 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    550 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 15 w/v -
    • Ammonium sulfate 0.15 M -
    pH 8
    42 μl of Ammonium sulfate (3.5 M)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    557 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 4.5
    8 μl of tri-Sodium citrate pH 3 (1.6 M)
    54 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    537 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 5
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium acetate 0.2 M -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    200 μl of Ammonium acetate (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    C4
    • MES 0.1 M pH 6
    • Polyethylene glycol 4,000 20 w/v -
    • Lithium sulfate 0.2 M -
    pH 6
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 20 w/v -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    500 μl of water (100 v/v)
    C6
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium sulfate 0.15 M -
    pH 7
    42 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    457 μl of water (100 v/v)
    C7
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    59 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    337 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 20 w/v -
    • Sodium chloride 0.2 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    C9
    • Sodium cacodylate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium cacodylate pH 5.5 (1 M)
    400 μl of water (100 v/v)
    C10
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 4,000 25 w/v -
    • Ammonium sulfate 0.15 M -
    pH 5.5
    177 μl of MES pH 5.2 (0.5 M)
    42 μl of Ammonium sulfate (3.5 M)
    22 μl of MES pH 7.1 (0.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    257 μl of water (100 v/v)
    C11
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    C12
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 25 w/v -
    • Potassium iodide 0.2 M -
    pH 6.5
    200 μl of Potassium iodide (1 M)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium chloride 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    D2
    • MES 0.1 M pH 6.5
    • 1-Propanol 12 v/v -
    • Polyethylene glycol monomethyl ether 5,000 10 w/v -
    pH 6.5
    300 μl of 1-Propanol (40 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    300 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 5,000 15 w/v -
    • Potassium chloride 0.1 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    575 μl of water (100 v/v)
    D4
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    • Ammonium sulfate 0.2 M -
    pH 7.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    442 μl of water (100 v/v)
    D5
    • MES 0.1 M pH 6
    • Polyethylene glycol 6,000 8 w/v -
    • Magnesium chloride 0.1 M -
    pH 6
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    590 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 8 w/v -
    • Sodium chloride 0.15 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    30 μl of Sodium chloride (5 M)
    710 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 6,000 15 w/v -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    637 μl of water (100 v/v)
    D8
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 6,000 15 w/v -
    • Magnesium acetate 0.1 M -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Magnesium acetate (1 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    D9
    • MES 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Polyethylene glycol 6,000 15 w/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    450 μl of water (100 v/v)
    D10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 15 w/v -
    • Potassium chloride 0.1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    575 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 6,000 15 w/v -
    pH 7.5
    100 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    600 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    E1
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 8,000 8 w/v -
    • Magnesium acetate 0.1 M -
    pH 4.5
    58 μl of Sodium acetate pH 4 (1 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    41 μl of Sodium acetate pH 5 (1 M)
    100 μl of Magnesium acetate (1 M)
    640 μl of water (100 v/v)
    E2
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 8,000 8 w/v -
    pH 5
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    777 μl of water (100 v/v)
    E3
    • Sodium cacodylate 0.1 M pH 6
    • Polyethylene glycol 8,000 8 w/v -
    • Sodium chloride 0.2 M -
    pH 6
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6 (1 M)
    40 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 8,000 8 w/v -
    pH 7
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    740 μl of water (100 v/v)
    E5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 8 w/v -
    pH 8
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    E6
    • Sodium cacodylate 0.1 M pH 5.5
    • Polyethylene glycol 8,000 12 w/v -
    • Calcium acetate 0.1 M -
    pH 5.5
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Calcium acetate (1 M)
    100 μl of Sodium cacodylate pH 5.5 (1 M)
    560 μl of water (100 v/v)
    E7
    • Sodium di-hydrogen phosphate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 12 w/v -
    pH 6.5
    16 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    3 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    740 μl of water (100 v/v)
    E8
    • MOPS 0.1 M pH 7.5
    • Polyethylene glycol 8,000 12 w/v -
    • Magnesium acetate 0.1 M -
    pH 7.5
    25 μl of MOPS pH 6.5 (1 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    74 μl of MOPS pH 7.9 (1 M)
    100 μl of Magnesium acetate (1 M)
    560 μl of water (100 v/v)
    E9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 12 w/v -
    • Sodium chloride 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    620 μl of water (100 v/v)
    E10
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 12 w/v -
    • Ammonium sulfate 0.2 M -
    pH 8.5
    57 μl of Ammonium sulfate (3.5 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    602 μl of water (100 v/v)
    E11
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 8,000 20 w/v -
    pH 5
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    537 μl of water (100 v/v)
    E12
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    • Ammonium sulfate 0.2 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    57 μl of Ammonium sulfate (3.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    151 μl of MES pH 7.1 (0.5 M)
    342 μl of water (100 v/v)
    F1
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 8,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    500 μl of water (100 v/v)
    F2
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 20 w/v -
    • Lithium chloride 0.2 M -
    pH 8
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    20 μl of Lithium chloride (10 M)
    480 μl of water (100 v/v)
    F3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 10,000 10 w/v -
    • Magnesium acetate 0.1 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    151 μl of MES pH 7.1 (0.5 M)
    100 μl of Magnesium acetate (1 M)
    500 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 12,000 18 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Polyethylene glycol 12,000 (30 w/v)
    300 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 20,000 8 w/v -
    • Sodium chloride 0.1 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    20 μl of Sodium chloride (5 M)
    613 μl of water (100 v/v)
    F6
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 20,000 15 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    500 μl of Polyethylene glycol 20,000 (30 w/v)
    400 μl of water (100 v/v)
    F7
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.5 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    142 μl of Ammonium sulfate (3.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    657 μl of water (100 v/v)
    F8
    • Sodium acetate 0.1 M pH 5
    • Ammonium sulfate 1 M -
    pH 5
    285 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5 (1 M)
    614 μl of water (100 v/v)
    F9
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    285 μl of Ammonium sulfate (3.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    514 μl of water (100 v/v)
    F10
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1 M -
    pH 8
    285 μl of Ammonium sulfate (3.5 M)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    614 μl of water (100 v/v)
    F11
    • Sodium acetate 0.1 M pH 5
    • Ammonium sulfate 1.5 M -
    pH 5
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5 (1 M)
    471 μl of water (100 v/v)
    F12
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1.5 M -
    pH 7
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7 (1 M)
    471 μl of water (100 v/v)
    G1
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1.5 M -
    pH 8
    428 μl of Ammonium sulfate (3.5 M)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    471 μl of water (100 v/v)
    G2
    • Sodium acetate 0.1 M pH 5
    • Ammonium sulfate 2 M -
    pH 5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 5 (1 M)
    328 μl of water (100 v/v)
    G3
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2 M -
    pH 7
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7 (1 M)
    328 μl of water (100 v/v)
    G4
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 2 M -
    pH 8
    571 μl of Ammonium sulfate (3.5 M)
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    G5
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1 M -
    • Potassium chloride 1 M -
    pH 7
    285 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7 (1 M)
    250 μl of Potassium chloride (4 M)
    364 μl of water (100 v/v)
    G6
    • Sodium acetate 0.1 M pH 5
    • Sodium formate 2 M -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    285 μl of Sodium formate (7 M)
    614 μl of water (100 v/v)
    G7
    • tris chloride BCC 0.1 M pH 7.5
    • Sodium formate 3 M -
    pH 7.5
    100 μl of tris chloride BCC pH 7.5 (1 M)
    428 μl of Sodium formate (7 M)
    471 μl of water (100 v/v)
    G8
    • Sodium Potassium phosphate 0.8 M pH 7.5
    pH 7.5
    266 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    733 μl of water (100 v/v)
    G9
    • Sodium Potassium phosphate 1.3 M pH 7
    pH 7
    433 μl of Sodium Potassium phosphate pH 7 (3 M)
    566 μl of water (100 v/v)
    G10
    • Sodium Potassium phosphate 1.6 M pH 6.5
    pH 6.5
    46 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    486 μl of Sodium Potassium phosphate pH 7 (3 M)
    466 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of Sodium acetate (3 M)
    566 μl of water (100 v/v)
    G12
    • HEPES 0.1 M pH 7
    • tri-Sodium citrate 1 M -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    333 μl of tri-Sodium citrate (3 M)
    566 μl of water (100 v/v)
    H1
    • tri-Sodium citrate 0.1 M pH 6
    • Sodium chloride 2 M -
    pH 6
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Sodium chloride (5 M)
    537 μl of water (100 v/v)
    H2
    • MES 0.1 M pH 6.5
    • Lithium sulfate 1 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    500 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    H3
    • tris chloride BCC 0.1 M pH 8
    • Lithium sulfate 1.6 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of Lithium sulfate (2 M)
    100 μl of water (100 v/v)
    H4
    • di-Sodium malonate 1.4 M pH 6
    pH 6
    411 μl of di-Sodium malonate pH 6 (3.4 M)
    588 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8
    • Potassium Sodium tartrate 1.2 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of water (100 v/v)
    H6
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    640 μl of Magnesium sulfate (2.5 M)
    160 μl of water (100 v/v)
    H7
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • Polyethylene glycol 4,000 2 w/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    40 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    710 μl of water (100 v/v)
    H8
    • Sodium cacodylate 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 25 v/v -
    • Calcium acetate 0.05 M -
    pH 6
    50 μl of Calcium acetate (1 M)
    100 μl of Sodium cacodylate pH 6 (1 M)
    250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    600 μl of water (100 v/v)
    H9
    • Imidazole 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 7
    47 μl of Imidazole pH 8 (1 M)
    53 μl of Imidazole pH 6.2 (1 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    H10
    • MES 0.1 M pH 6.5
    • 2-Propanol 10 v/v -
    • Polyethylene glycol 4,000 5 w/v -
    • Magnesium chloride 0.05 M -
    pH 6.5
    25 μl of Magnesium chloride (2 M)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    575 μl of water (100 v/v)
    H11
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 25 v/v -
    • Ammonium acetate 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    250 μl of 2-Propanol (100 v/v)
    450 μl of water (100 v/v)
    H12
    • tris chloride BCC 0.1 M pH 8
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Ethanol 15 v/v -
    • Sodium chloride 0.1 M -
    pH 8
    53 μl of tris chloride BCC pH 7.5 (1 M)
    47 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Ethanol (100 v/v)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    20 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)