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Supplemental Information

Screen Description

ProPlex

Created 2009-11-16.

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ProPlex

No barcodes found.

Screen Structure

ProPlex

Well Count 96
Number of distinct ingredients 43
Internal diversity (score mean dist) 0.919

Screen Composition

2025-08-16T01:43:05.877480 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type ProPlex
Buffer 113
Organic (non-volatile) 8
Organic (volatile) 6
Polymer 68
Salt 98

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
ProPlex Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 350 25 v/v -
pH 8
250.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
A2
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 15 v/v -
  • Calcium acetate 0.1 M -
pH 6
100.0 μl of Calcium acetate (1 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
A3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 20 v/v -
  • Lithium chloride 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
690.0 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 25 v/v -
pH 8
250.0 μl of Polyethylene glycol 400 (100 v/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
A5
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 15 v/v -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
150.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
650.0 μl of water (100 v/v)
A6
  • Sodium Potassium phosphate 0.1 M pH 6.5
  • Polyethylene glycol 1,000 25 w/v -
  • Sodium chloride 0.2 M -
pH 6.5
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
15.6 μl of Sodium Potassium phosphate pH 6.2 (3 M)
17.8 μl of Sodium Potassium phosphate pH 7 (3 M)
40.0 μl of Sodium chloride (5 M)
426.7 μl of water (100 v/v)
A7
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 1,500 20 w/v -
  • Ammonium sulfate 0.1 M -
pH 7.5
28.6 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
471.4 μl of water (100 v/v)
A8
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
  • Ammonium sulfate 0.2 M -
pH 5.5
57.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
642.9 μl of water (100 v/v)
A9
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Sodium chloride 0.2 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
A10
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • Potassium chloride 0.1 M -
pH 8
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
575.0 μl of water (100 v/v)
A11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
400.0 μl of water (100 v/v)
A12
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 5 w/v -
  • Sodium acetate 0.2 M -
pH 5.5
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
770.8 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 4,000 5 w/v -
  • Lithium sulfate 0.2 M -
pH 7.5
100.0 μl of Lithium sulfate (2 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
700.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 10 w/v -
  • Calcium acetate 0.1 M -
pH 4.5
100.0 μl of Calcium acetate (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
B3
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 10 w/v -
  • Sodium acetate 0.2 M -
pH 5.5
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
670.8 μl of water (100 v/v)
B4
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 10 w/v -
  • Sodium chloride 0.2 M -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 10 w/v -
  • Magnesium chloride 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 10 w/v -
pH 7
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
B7
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 4,000 15 w/v -
  • Ammonium acetate 0.2 M -
pH 4
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
400.0 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 4,000 15 w/v -
  • Magnesium chloride 0.1 M -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
587.5 μl of water (100 v/v)
B9
  • Sodium cacodylate 0.1 M pH 6
  • Polyethylene glycol 4,000 15 w/v -
pH 6
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6 (1 M)
600.0 μl of water (100 v/v)
B10
  • MES 0.1 M pH 6
  • Polyethylene glycol 4,000 15 w/v -
  • Ammonium sulfate 0.15 M -
pH 6
42.9 μl of Ammonium sulfate (3.5 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
457.1 μl of water (100 v/v)
B11
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 4,000 15 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
B12
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 4,000 15 w/v -
  • Magnesium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
550.0 μl of water (100 v/v)
C1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 15 w/v -
  • Ammonium sulfate 0.15 M -
pH 8
42.9 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
557.1 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 20 w/v -
pH 4.5
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
35.3 μl of tri-Sodium citrate pH 3 (1.6 M)
27.2 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 5
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium acetate 0.2 M -
pH 5
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
300.0 μl of water (100 v/v)
C4
  • MES 0.1 M pH 6
  • Polyethylene glycol 4,000 20 w/v -
  • Lithium sulfate 0.2 M -
pH 6
100.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
C5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 20 w/v -
pH 8
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C6
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.15 M -
pH 7
42.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
457.1 μl of water (100 v/v)
C7
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
21.1 μl of tri-Sodium citrate pH 3 (1.6 M)
41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
337.5 μl of water (100 v/v)
C8
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 20 w/v -
  • Sodium chloride 0.2 M -
pH 8
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
460.0 μl of water (100 v/v)
C9
  • Sodium cacodylate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 25 w/v -
pH 5.5
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
C10
  • MES 0.1 M pH 5.5
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.15 M -
pH 5.5
42.9 μl of Ammonium sulfate (3.5 M)
177.7 μl of MES pH 5.2 (0.5 M)
22.3 μl of MES pH 7.1 (0.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
257.1 μl of water (100 v/v)
C11
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 4,000 25 w/v -
pH 6.5
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
C12
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 25 w/v -
  • Potassium iodide 0.2 M -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Potassium iodide (1 M)
100.0 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
D2
  • MES 0.1 M pH 6.5
  • 1-Propanol 12 v/v -
  • Polyethylene glycol monomethyl ether 5,000 10 w/v -
pH 6.5
300.0 μl of 1-Propanol (40 v/v)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
300.0 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 5,000 15 w/v -
  • Potassium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
575.0 μl of water (100 v/v)
D4
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • Ammonium sulfate 0.2 M -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
442.9 μl of water (100 v/v)
D5
  • MES 0.1 M pH 6
  • Polyethylene glycol 6,000 8 w/v -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
160.0 μl of Polyethylene glycol 6,000 (50 w/v)
590.0 μl of water (100 v/v)
D6
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 6,000 8 w/v -
  • Sodium chloride 0.15 M -
pH 8
160.0 μl of Polyethylene glycol 6,000 (50 w/v)
30.0 μl of Sodium chloride (5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
710.0 μl of water (100 v/v)
D7
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 6,000 15 w/v -
pH 5.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
D8
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 6,000 15 w/v -
  • Magnesium acetate 0.1 M -
pH 6.5
100.0 μl of Magnesium acetate (1 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
D9
  • MES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • Polyethylene glycol 6,000 15 w/v -
pH 6.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
450.0 μl of water (100 v/v)
D10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 15 w/v -
  • Potassium chloride 0.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
575.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 6,000 15 w/v -
pH 7.5
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
600.0 μl of water (100 v/v)
D12
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
E1
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 8,000 8 w/v -
  • Magnesium acetate 0.1 M -
pH 4.5
100.0 μl of Magnesium acetate (1 M)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
640.0 μl of water (100 v/v)
E2
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 8,000 8 w/v -
pH 5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
777.5 μl of water (100 v/v)
E3
  • Sodium cacodylate 0.1 M pH 6
  • Polyethylene glycol 8,000 8 w/v -
  • Sodium chloride 0.2 M -
pH 6
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6 (1 M)
40.0 μl of Sodium chloride (5 M)
700.0 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 8,000 8 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
740.0 μl of water (100 v/v)
E5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 8,000 8 w/v -
pH 8
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
E6
  • Sodium cacodylate 0.1 M pH 5.5
  • Polyethylene glycol 8,000 12 w/v -
  • Calcium acetate 0.1 M -
pH 5.5
100.0 μl of Calcium acetate (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 5.5 (1 M)
560.0 μl of water (100 v/v)
E7
  • Sodium di-hydrogen phosphate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 12 w/v -
pH 6.5
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
16.9 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
3.1 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
740.0 μl of water (100 v/v)
E8
  • MOPS 0.1 M pH 7.5
  • Polyethylene glycol 8,000 12 w/v -
  • Magnesium acetate 0.1 M -
pH 7.5
25.1 μl of MOPS pH 6.5 (1 M)
74.9 μl of MOPS pH 7.9 (1 M)
100.0 μl of Magnesium acetate (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
560.0 μl of water (100 v/v)
E9
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 12 w/v -
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
E10
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 12 w/v -
  • Ammonium sulfate 0.2 M -
pH 8.5
57.1 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
602.9 μl of water (100 v/v)
E11
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 8,000 20 w/v -
pH 5
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
E12
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
  • Ammonium sulfate 0.2 M -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.9 μl of water (100 v/v)
F1
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 8,000 20 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
F2
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 8,000 20 w/v -
  • Lithium chloride 0.2 M -
pH 8
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
480.0 μl of water (100 v/v)
F3
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 10,000 10 w/v -
  • Magnesium acetate 0.1 M -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
100.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 12,000 18 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Polyethylene glycol 12,000 (30 w/v)
300.0 μl of water (100 v/v)
F5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 20,000 8 w/v -
  • Sodium chloride 0.1 M -
pH 8
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
20.0 μl of Sodium chloride (5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
613.3 μl of water (100 v/v)
F6
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 20,000 15 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
500.0 μl of Polyethylene glycol 20,000 (30 w/v)
400.0 μl of water (100 v/v)
F7
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.5 M -
pH 6.5
142.9 μl of Ammonium sulfate (3.5 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
657.1 μl of water (100 v/v)
F8
  • Sodium acetate 0.1 M pH 5
  • Ammonium sulfate 1 M -
pH 5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 5 (1 M)
614.3 μl of water (100 v/v)
F9
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
285.7 μl of Ammonium sulfate (3.5 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
514.3 μl of water (100 v/v)
F10
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 1 M -
pH 8
285.7 μl of Ammonium sulfate (3.5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
614.3 μl of water (100 v/v)
F11
  • Sodium acetate 0.1 M pH 5
  • Ammonium sulfate 1.5 M -
pH 5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 5 (1 M)
471.4 μl of water (100 v/v)
F12
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 1.5 M -
pH 7
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7 (1 M)
471.4 μl of water (100 v/v)
G1
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 1.5 M -
pH 8
428.6 μl of Ammonium sulfate (3.5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
G2
  • Sodium acetate 0.1 M pH 5
  • Ammonium sulfate 2 M -
pH 5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 5 (1 M)
328.6 μl of water (100 v/v)
G3
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 2 M -
pH 7
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7 (1 M)
328.6 μl of water (100 v/v)
G4
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 2 M -
pH 8
571.4 μl of Ammonium sulfate (3.5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
G5
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 1 M -
  • Potassium chloride 1 M -
pH 7
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7 (1 M)
250.0 μl of Potassium chloride (4 M)
364.3 μl of water (100 v/v)
G6
  • Sodium acetate 0.1 M pH 5
  • Sodium formate 2 M -
pH 5
100.0 μl of Sodium acetate pH 5 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
G7
  • tris chloride BCC 0.1 M pH 7.5
  • Sodium formate 3 M -
pH 7.5
428.6 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
471.4 μl of water (100 v/v)
G8
  • Sodium Potassium phosphate 0.8 M pH 7.5
pH 7.5
266.7 μl of Sodium Potassium phosphate pH 7.5 (3 M)
733.3 μl of water (100 v/v)
G9
  • Sodium Potassium phosphate 1.3 M pH 7
pH 7
433.3 μl of Sodium Potassium phosphate pH 7 (3 M)
566.7 μl of water (100 v/v)
G10
  • Sodium Potassium phosphate 1.6 M pH 6.5
pH 6.5
248.8 μl of Sodium Potassium phosphate pH 6.2 (3 M)
284.5 μl of Sodium Potassium phosphate pH 7 (3 M)
466.7 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
333.3 μl of Sodium acetate (3 M)
566.7 μl of water (100 v/v)
G12
  • HEPES 0.1 M pH 7
  • tri-Sodium citrate 1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
333.3 μl of tri-Sodium citrate (3 M)
566.7 μl of water (100 v/v)
H1
  • tri-Sodium citrate 0.1 M pH 6
  • Sodium chloride 2 M -
pH 6
400.0 μl of Sodium chloride (5 M)
14.1 μl of tri-Sodium citrate pH 3 (1.6 M)
48.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
H2
  • MES 0.1 M pH 6.5
  • Lithium sulfate 1 M -
pH 6.5
500.0 μl of Lithium sulfate (2 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
300.0 μl of water (100 v/v)
H3
  • tris chloride BCC 0.1 M pH 8
  • Lithium sulfate 1.6 M -
pH 8
800.0 μl of Lithium sulfate (2 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
H4
  • di-Sodium malonate 1.4 M pH 6
pH 6
411.8 μl of di-Sodium malonate pH 6 (3.4 M)
588.2 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8
  • Potassium Sodium tartrate 1.2 M -
pH 8
800.0 μl of Potassium Sodium tartrate (1.5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
H6
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
640.0 μl of Magnesium sulfate (2.5 M)
160.0 μl of water (100 v/v)
H7
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • Polyethylene glycol 4,000 2 w/v -
pH 5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
710.0 μl of water (100 v/v)
H8
  • Sodium cacodylate 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • Calcium acetate 0.05 M -
pH 6
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Calcium acetate (1 M)
100.0 μl of Sodium cacodylate pH 6 (1 M)
600.0 μl of water (100 v/v)
H9
  • Imidazole 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 7
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
53.0 μl of Imidazole pH 6.2 (1 M)
47.0 μl of Imidazole pH 8 (1 M)
400.0 μl of water (100 v/v)
H10
  • MES 0.1 M pH 6.5
  • 2-Propanol 10 v/v -
  • Polyethylene glycol 4,000 5 w/v -
  • Magnesium chloride 0.05 M -
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
25.0 μl of Magnesium chloride (2 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
575.0 μl of water (100 v/v)
H11
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 25 v/v -
  • Ammonium acetate 0.2 M -
pH 7.5
250.0 μl of 2-Propanol (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
450.0 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • Ethanol 15 v/v -
  • Sodium chloride 0.1 M -
pH 8
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
150.0 μl of Ethanol (100 v/v)
20.0 μl of Sodium chloride (5 M)
53.0 μl of tris chloride BCC pH 7.5 (1 M)
47.0 μl of tris chloride BCC pH 8.5 (1 M)
680.0 μl of water (100 v/v)