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Supplemental Information

Screen Description

Stura Footprint

Molecular Dimensions / Stura Footprint
Created 2009-11-16.

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Screen Structure

Stura Footprint

Well Count 48
Number of distinct ingredients 16
Internal diversity (score mean dist) 0.896

Screen Composition

2025-08-16T09:05:39.776185 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Stura Footprint
Buffer 52
Organic (non-volatile) 8
Polymer 36
Salt 28

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Stura Footprint Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 15 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
150.0 μl of Polyethylene glycol 600 (100 v/v)
750.0 μl of water (100 v/v)
A2
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 24 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
240.0 μl of Polyethylene glycol 600 (100 v/v)
660.0 μl of water (100 v/v)
A3
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 33 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
330.0 μl of Polyethylene glycol 600 (100 v/v)
570.0 μl of water (100 v/v)
A4
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 42 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
420.0 μl of Polyethylene glycol 600 (100 v/v)
480.0 μl of water (100 v/v)
A5
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 10 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
700.0 μl of water (100 v/v)
A6
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 15 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
A7
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 25 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
B1
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 7.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
150.0 μl of Polyethylene glycol 10,000 (50 w/v)
750.0 μl of water (100 v/v)
B2
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 12.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
250.0 μl of Polyethylene glycol 10,000 (50 w/v)
650.0 μl of water (100 v/v)
B3
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 17.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
350.0 μl of Polyethylene glycol 10,000 (50 w/v)
550.0 μl of water (100 v/v)
B4
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 22.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
450.0 μl of Polyethylene glycol 10,000 (50 w/v)
450.0 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 0.75 M -
pH 5.5
214.3 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
692.0 μl of water (100 v/v)
B6
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 1 M -
pH 5.5
285.7 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
620.5 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 1.5 M -
pH 5.5
428.6 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
477.7 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
334.8 μl of water (100 v/v)
C1
  • Sodium Potassium phosphate 0.8 M pH 7
pH 7
266.7 μl of Sodium Potassium phosphate pH 7 (3 M)
733.3 μl of water (100 v/v)
C2
  • Sodium Potassium phosphate 1.32 M pH 7
pH 7
440.0 μl of Sodium Potassium phosphate pH 7 (3 M)
560.0 μl of water (100 v/v)
C3
  • Sodium Potassium phosphate 1.6 M pH 7
pH 7
533.3 μl of Sodium Potassium phosphate pH 7 (3 M)
466.7 μl of water (100 v/v)
C4
  • Sodium Potassium phosphate 2 M pH 7
pH 7
666.7 μl of Sodium Potassium phosphate pH 7 (3 M)
333.3 μl of water (100 v/v)
C5
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 0.75 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
468.8 μl of tri-Sodium citrate (1.6 M)
521.2 μl of water (100 v/v)
C6
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
365.0 μl of water (100 v/v)
C7
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1.2 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
750.0 μl of tri-Sodium citrate (1.6 M)
240.0 μl of water (100 v/v)
C8
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1.5 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
937.5 μl of tri-Sodium citrate (1.6 M)
52.5 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
600.0 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 40 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
500.0 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 50 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
500.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
400.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 60 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
300.0 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 18 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
180.0 μl of Polyethylene glycol 600 (100 v/v)
720.0 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 27 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
270.0 μl of Polyethylene glycol 600 (100 v/v)
630.0 μl of water (100 v/v)
D7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 36 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 600 (100 v/v)
540.0 μl of water (100 v/v)
D8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 45 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
450.0 μl of Polyethylene glycol 600 (100 v/v)
450.0 μl of water (100 v/v)
E1
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
540.0 μl of water (100 v/v)
E2
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 27 w/v -
pH 6.5
540.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
360.0 μl of water (100 v/v)
E3
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 36 w/v -
pH 6.5
720.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
180.0 μl of water (100 v/v)
E4
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 45 w/v -
pH 6.5
900.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
E5
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 8 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
740.0 μl of water (100 v/v)
E6
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 15 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
E7
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 20 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
E8
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 30 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
F1
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 12 w/v -
pH 5.5
240.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
660.0 μl of water (100 v/v)
F2
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 5.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
540.0 μl of water (100 v/v)
F3
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 24 w/v -
pH 5.5
480.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
420.0 μl of water (100 v/v)
F4
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 36 w/v -
pH 5.5
720.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
180.0 μl of water (100 v/v)
F5
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 9 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
180.0 μl of Polyethylene glycol 10,000 (50 w/v)
720.0 μl of water (100 v/v)
F6
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 15 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
600.0 μl of water (100 v/v)
F7
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 22.5 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
450.0 μl of Polyethylene glycol 10,000 (50 w/v)
450.0 μl of water (100 v/v)
F8
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 27 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
540.0 μl of Polyethylene glycol 10,000 (50 w/v)
360.0 μl of water (100 v/v)