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Supplemental Information

Screen Description

Molecular Dimensions / Mini screen
Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count 24
Number of distinct ingredients 24
Internal diversity (Score mean dist)0.94
Internal diversity (Ion mean dist)0.92
Internal diversity (PEG mean dist)0.91
Internal diversity (Known pH mean dist)0.85
Internal diversity (Penalty mean dist)0.92

Screen Composition

More information about screen composition chart.
Chemical classes bar chart
ingredient type Mini screen
Buffer 29
Organic (non-volatile) 5
Organic (volatile) 1
Polymer 15
Salt 29

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Mini screen Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
 pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
590.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
 pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
100.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
 pH 4.6
57.14 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
342.86 μl of water (100 v/v)
A4
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
 pH 4.6
47.5 μl of Sodium acetate pH 4 (1 M)
52.5 μl of Sodium acetate pH 5 (1 M)
285.71 μl of Sodium formate (7 M)
614.29 μl of water (100 v/v)
A5
  • Ammonium di-hydrogen phosphate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
 pH 5.6
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
11.61 μl of tri-Sodium citrate pH 3 (1.6 M)
50.89 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
A6
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
 pH 6.5
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
 pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Magnesium acetate (1 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
 pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.67 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
233.33 μl of water (100 v/v)
B3
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 8,000 18 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
 pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of Zinc acetate (1 M)
340.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
 pH 7.5
100.0 μl of Calcium chloride (2 M)
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
B5
  • Potassium Sodium phosphate 1.6 M -
  • HEPES 0.1 M pH 7.5
 pH 7.5
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
533.33 μl of Potassium Sodium phosphate pH 7 (3 M)
366.67 μl of water (100 v/v)
B6
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
 pH 7.5
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
C1
  • tri-Sodium citrate 1.4 M -
  • HEPES 0.1 M pH 7.5
 pH 7.5
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
466.67 μl of tri-Sodium citrate (3 M)
433.33 μl of water (100 v/v)
C2
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 2 v/v -
  • HEPES 0.1 M pH 7.5
 pH 7.5
571.43 μl of Ammonium sulfate (3.5 M)
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
308.57 μl of water (100 v/v)
C3
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
 pH 7.5
100.0 μl of 2-Propanol (100 v/v)
0.0 μl of HEPES pH 5 (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
C4
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
 pH 8.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.57 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 30 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
 pH 8.5
100.0 μl of Magnesium chloride (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
 pH 8.5
300.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
475.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
 pH 8.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 8,000 8 w/v -
  • tris chloride BCC 0.1 M pH 8.5
 pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
 NA
57.14 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.86 μl of water (100 v/v)
D4
  • Sodium formate 4 M -
 NA
571.43 μl of Sodium formate (7 M)
428.57 μl of water (100 v/v)
D5
  • Polyethylene glycol 8,000 20 w/v -
  • Potassium di-hydrogen phosphate 0.05 M -
 NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium di-hydrogen phosphate (2 M)
575.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 1,500 30 w/v -
 NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)