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Supplemental Information

Screen Description

Stura+MacroSol HT

Molecular Dimensions / Stura+MacroSol HT
Created 2009-11-16.

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Stura+MacroSol HT

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Screen Structure

Stura+MacroSol HT

Well Count 96
Number of distinct ingredients 30
Internal diversity (score mean dist) 0.933

Screen Composition

2025-08-16T06:25:42.032565 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Stura+MacroSol HT
Buffer 106
Organic (non-volatile) 16
Organic (volatile) 8
Polymer 65
Salt 98

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Stura+MacroSol HT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 15 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
150.0 μl of Polyethylene glycol 600 (100 v/v)
750.0 μl of water (100 v/v)
A2
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 24 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
240.0 μl of Polyethylene glycol 600 (100 v/v)
660.0 μl of water (100 v/v)
A3
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 33 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
330.0 μl of Polyethylene glycol 600 (100 v/v)
570.0 μl of water (100 v/v)
A4
  • Imidazole-Malate 0.2 M pH 5.5
  • Polyethylene glycol 600 42 v/v -
pH 5.5
100.0 μl of Imidazole-Malate pH 5.5 (2 M)
420.0 μl of Polyethylene glycol 600 (100 v/v)
480.0 μl of water (100 v/v)
A5
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 10 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
700.0 μl of water (100 v/v)
A6
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 15 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
A7
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Imidazole-Malate 0.2 M pH 7
  • Polyethylene glycol 4,000 25 w/v -
pH 7
2.0 μl of Imidazole-Malate pH 5.5 (2 M)
98.0 μl of Imidazole-Malate pH 7.5 (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
A9
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 7.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
150.0 μl of Polyethylene glycol 10,000 (50 w/v)
750.0 μl of water (100 v/v)
A10
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 12.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
250.0 μl of Polyethylene glycol 10,000 (50 w/v)
650.0 μl of water (100 v/v)
A11
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 17.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
350.0 μl of Polyethylene glycol 10,000 (50 w/v)
550.0 μl of water (100 v/v)
A12
  • Imidazole-Malate 0.2 M pH 8
  • Polyethylene glycol 10,000 22.5 w/v -
pH 8
2.0 μl of Imidazole-Malate pH 7.5 (2 M)
98.0 μl of Imidazole-Malate pH 8.5 (2 M)
450.0 μl of Polyethylene glycol 10,000 (50 w/v)
450.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 0.75 M -
pH 5.5
214.3 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
692.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 1 M -
pH 5.5
285.7 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
620.5 μl of water (100 v/v)
B3
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 1.5 M -
pH 5.5
428.6 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
477.7 μl of water (100 v/v)
B4
  • tri-Sodium citrate 0.15 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
34.0 μl of tri-Sodium citrate pH 3 (1.6 M)
59.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
334.8 μl of water (100 v/v)
B5
  • Sodium Potassium phosphate 0.8 M pH 7
pH 7
266.7 μl of Sodium Potassium phosphate pH 7 (3 M)
733.3 μl of water (100 v/v)
B6
  • Sodium Potassium phosphate 1.32 M pH 7
pH 7
440.0 μl of Sodium Potassium phosphate pH 7 (3 M)
560.0 μl of water (100 v/v)
B7
  • Sodium Potassium phosphate 1.6 M pH 7
pH 7
533.3 μl of Sodium Potassium phosphate pH 7 (3 M)
466.7 μl of water (100 v/v)
B8
  • Sodium Potassium phosphate 2 M pH 7
pH 7
666.7 μl of Sodium Potassium phosphate pH 7 (3 M)
333.3 μl of water (100 v/v)
B9
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 0.75 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
468.8 μl of tri-Sodium citrate (1.6 M)
521.2 μl of water (100 v/v)
B10
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
365.0 μl of water (100 v/v)
B11
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1.2 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
750.0 μl of tri-Sodium citrate (1.6 M)
240.0 μl of water (100 v/v)
B12
  • di-Sodium tetraborate 0.01 M pH 8.5
  • tri-Sodium citrate 1.5 M -
pH 8.5
10.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
937.5 μl of tri-Sodium citrate (1.6 M)
52.5 μl of water (100 v/v)
C1
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
600.0 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 40 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
500.0 μl of water (100 v/v)
C3
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 50 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
500.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
400.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 8.2
  • Polyethylene glycol monomethyl ether 550 60 v/v -
pH 8.2
31.0 μl of HEPES pH 7.8 (1 M)
69.0 μl of HEPES pH 8.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
300.0 μl of water (100 v/v)
C5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 18 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
180.0 μl of Polyethylene glycol 600 (100 v/v)
720.0 μl of water (100 v/v)
C6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 27 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
270.0 μl of Polyethylene glycol 600 (100 v/v)
630.0 μl of water (100 v/v)
C7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 36 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Polyethylene glycol 600 (100 v/v)
540.0 μl of water (100 v/v)
C8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 600 45 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
450.0 μl of Polyethylene glycol 600 (100 v/v)
450.0 μl of water (100 v/v)
C9
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
540.0 μl of water (100 v/v)
C10
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 27 w/v -
pH 6.5
540.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
360.0 μl of water (100 v/v)
C11
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 36 w/v -
pH 6.5
720.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
180.0 μl of water (100 v/v)
C12
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 45 w/v -
pH 6.5
900.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
D1
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 8 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
740.0 μl of water (100 v/v)
D2
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 15 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
D3
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 20 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
D4
  • Imidazole-Malate 0.2 M pH 6
  • Polyethylene glycol 4,000 30 w/v -
pH 6
90.9 μl of Imidazole-Malate pH 5.5 (2 M)
9.1 μl of Imidazole-Malate pH 7.5 (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
D5
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 12 w/v -
pH 5.5
240.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
660.0 μl of water (100 v/v)
D6
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 5.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
540.0 μl of water (100 v/v)
D7
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 24 w/v -
pH 5.5
480.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
420.0 μl of water (100 v/v)
D8
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 36 w/v -
pH 5.5
720.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
180.0 μl of water (100 v/v)
D9
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 9 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
180.0 μl of Polyethylene glycol 10,000 (50 w/v)
720.0 μl of water (100 v/v)
D10
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 15 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
600.0 μl of water (100 v/v)
D11
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 22.5 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
450.0 μl of Polyethylene glycol 10,000 (50 w/v)
450.0 μl of water (100 v/v)
D12
  • Ammonium acetate 0.1 M pH 4.5
  • Polyethylene glycol 10,000 27 w/v -
pH 4.5
90.9 μl of Ammonium acetate pH 4 (1 M)
9.1 μl of Ammonium acetate pH 10 (1 M)
540.0 μl of Polyethylene glycol 10,000 (50 w/v)
360.0 μl of water (100 v/v)
E1
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
790.0 μl of water (100 v/v)
E2
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
690.0 μl of water (100 v/v)
E3
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
590.0 μl of water (100 v/v)
E5
  • Sodium acetate 1 M -
  • Sodium di-hydrogen phosphate 0.1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
333.3 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
20.0 μl of Sodium di-hydrogen phosphate (5 M)
546.7 μl of water (100 v/v)
E6
  • Sodium acetate 1.4 M -
  • Sodium di-hydrogen phosphate 0.1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
466.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
20.0 μl of Sodium di-hydrogen phosphate (5 M)
413.3 μl of water (100 v/v)
E7
  • Sodium acetate 1.7 M -
  • Sodium di-hydrogen phosphate 0.1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
566.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
20.0 μl of Sodium di-hydrogen phosphate (5 M)
313.3 μl of water (100 v/v)
E8
  • Sodium acetate 1.7 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
566.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
333.3 μl of water (100 v/v)
E9
  • Polyethylene glycol 3,350 8 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Ammonium acetate (1 M)
160.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
540.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 3,350 15 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
400.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
300.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 3,350 30 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
100.0 μl of water (100 v/v)
F1
  • Ammonium di-hydrogen phosphate 0.75 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
300.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
F2
  • Ammonium di-hydrogen phosphate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
F3
  • Ammonium di-hydrogen phosphate 1.5 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
600.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
337.5 μl of water (100 v/v)
F4
  • Ammonium di-hydrogen phosphate 2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
137.5 μl of water (100 v/v)
F5
  • 2-Propanol 10 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
700.0 μl of water (100 v/v)
F6
  • 2-Propanol 15 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
150.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
650.0 μl of water (100 v/v)
F7
  • 2-Propanol 20 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
600.0 μl of water (100 v/v)
F8
  • 2-Propanol 30 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 3,350 16 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
480.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 3,350 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
250.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
NA
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
460.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
  • Imidazole-Malate 0.1 M pH 5.5
pH 5.5
50.0 μl of Imidazole-Malate pH 5.5 (2 M)
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
410.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
  • Imidazole-Malate 0.1 M pH 6.5
pH 6.5
45.5 μl of Imidazole-Malate pH 5.5 (2 M)
4.5 μl of Imidazole-Malate pH 7.5 (2 M)
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
410.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
  • Imidazole-Malate 0.1 M pH 7.5
pH 7.5
50.0 μl of Imidazole-Malate pH 7.5 (2 M)
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
410.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 8,000 12 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
702.9 μl of water (100 v/v)
G6
  • Polyethylene glycol 8,000 18 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
582.9 μl of water (100 v/v)
G7
  • Polyethylene glycol 8,000 24 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
480.0 μl of Polyethylene glycol 8,000 (50 w/v)
462.9 μl of water (100 v/v)
G8
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.9 μl of water (100 v/v)
G9
  • Polyethylene glycol 4,000 10 w/v -
  • Ammonium sulfate 0.5 M -
NA
142.9 μl of Ammonium sulfate (3.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
657.1 μl of water (100 v/v)
G10
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.3 M -
NA
85.7 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
514.3 μl of water (100 v/v)
G11
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
342.9 μl of water (100 v/v)
G12
  • Polyethylene glycol 4,000 36 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
720.0 μl of Polyethylene glycol 4,000 (50 w/v)
222.9 μl of water (100 v/v)
H1
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 400 2 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
594.3 μl of water (100 v/v)
H2
  • Ammonium sulfate 1.5 M -
  • Polypropylene glycol 400 2 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polypropylene glycol 400 (100 v/v)
451.4 μl of water (100 v/v)
H3
  • Ammonium sulfate 2 M -
  • Polypropylene glycol 400 2 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polypropylene glycol 400 (100 v/v)
308.6 μl of water (100 v/v)
H4
  • Ammonium sulfate 2 M -
  • Polypropylene glycol 400 5 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Polypropylene glycol 400 (100 v/v)
278.6 μl of water (100 v/v)
H5
  • Polyethylene glycol 4,000 12 w/v -
  • 2-Propanol 5 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
50.0 μl of 2-Propanol (100 v/v)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
647.5 μl of water (100 v/v)
H6
  • Polyethylene glycol 4,000 16 w/v -
  • 2-Propanol 10 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
100.0 μl of 2-Propanol (100 v/v)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
517.5 μl of water (100 v/v)
H7
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 15 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
150.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
387.5 μl of water (100 v/v)
H8
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
337.5 μl of water (100 v/v)
H9
  • Polyethylene glycol 8,000 9 w/v -
  • Zinc acetate 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
180.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
5.0 μl of Zinc acetate (1 M)
715.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 8,000 12 w/v -
  • Zinc acetate 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
5.0 μl of Zinc acetate (1 M)
655.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 18 w/v -
  • Zinc acetate 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
5.0 μl of Zinc acetate (1 M)
535.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 8,000 24 w/v -
  • Zinc acetate 0.005 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
480.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
5.0 μl of Zinc acetate (1 M)
415.0 μl of water (100 v/v)