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Supplemental Information

Screen Description

Cubic Phase I

Qiagen / Cubic Phase I
Created 2009-11-16.

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Cubic Phase I

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Cubic Phase I

    Screen Structure

    Cubic Phase I

    Well Count 96
    Number of distinct ingredients 7
    Internal diversity (score mean dist) 0.789

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:09:37.452123 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Cubic Phase I
    Buffer 96
    Salt 104

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Cubic Phase I Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium Potassium phosphate 0.6 M pH 4.6
    		pH 4.6
    200 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    800 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.8 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    A3
    • Ammonium di-hydrogen phosphate 1 M pH 4.6
    		pH 4.6
    357 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    642 μl of water (100 v/v)
    A4
    • Sodium Potassium phosphate 1.2 M pH 4.6
    		pH 4.6
    400 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    600 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 1.4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    350 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    A6
    • Ammonium di-hydrogen phosphate 1.6 M pH 4.6
    		pH 4.6
    571 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    428 μl of water (100 v/v)
    A7
    • Sodium Potassium phosphate 1.8 M pH 4.6
    		pH 4.6
    600 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    400 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    A9
    • Ammonium di-hydrogen phosphate 2.8 M pH 4.6
    		pH 4.6
    1000 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    A10
    • Sodium Potassium phosphate 2.4 M pH 4.6
    		pH 4.6
    800 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    200 μl of water (100 v/v)
    A11
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2.6 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    650 μl of Ammonium sulfate (4 M)
    250 μl of water (100 v/v)
    A12
    • Ammonium di-hydrogen phosphate 2.8 M pH 4.6
    		pH 4.6
    1000 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    B1
    • Sodium Potassium phosphate 0.6 M pH 5.2
    		pH 5.2
    112 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    87 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    800 μl of water (100 v/v)
    B2
    • Sodium acetate 0.1 M pH 5.2
    • Ammonium sulfate 0.8 M -
    		pH 5.2
    40 μl of Sodium acetate pH 5.6 (1 M)
    59 μl of Sodium acetate pH 5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    B3
    • Ammonium di-hydrogen phosphate 1 M pH 5.2
    		pH 5.2
    350 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    6 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    B4
    • Sodium Potassium phosphate 1.2 M pH 5.2
    		pH 5.2
    224 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    175 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    600 μl of water (100 v/v)
    B5
    • Sodium acetate 0.1 M pH 5.2
    • Ammonium sulfate 1.4 M -
    		pH 5.2
    40 μl of Sodium acetate pH 5.6 (1 M)
    59 μl of Sodium acetate pH 5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    B6
    • Ammonium di-hydrogen phosphate 1.6 M pH 5.2
    		pH 5.2
    560 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    11 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    B7
    • Sodium Potassium phosphate 1.8 M pH 5.2
    		pH 5.2
    336 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    263 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    400 μl of water (100 v/v)
    B8
    • Sodium acetate 0.1 M pH 5.2
    • Ammonium sulfate 2 M -
    		pH 5.2
    40 μl of Sodium acetate pH 5.6 (1 M)
    59 μl of Sodium acetate pH 5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    B9
    • Ammonium di-hydrogen phosphate 2.2 M pH 5.2
    		pH 5.2
    770 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    15 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    B10
    • Sodium Potassium phosphate 2.4 M pH 5.2
    		pH 5.2
    448 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    351 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    200 μl of water (100 v/v)
    B11
    • Sodium acetate 0.1 M pH 5.2
    • Ammonium sulfate 2.6 M -
    		pH 5.2
    40 μl of Sodium acetate pH 5.6 (1 M)
    59 μl of Sodium acetate pH 5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    250 μl of water (100 v/v)
    B12
    • Ammonium di-hydrogen phosphate 2.8 M pH 5.2
    		pH 5.2
    980 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    19 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    C1
    • Sodium Potassium phosphate 0.6 M pH 5.8
    		pH 5.8
    136 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    64 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    800 μl of water (100 v/v)
    C2
    • MES 0.1 M pH 5.8
    • Ammonium sulfate 0.8 M -
    		pH 5.8
    40 μl of MES pH 6.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    59 μl of MES pH 5 (1 M)
    700 μl of water (100 v/v)
    C3
    • Ammonium di-hydrogen phosphate 1 M pH 5.8
    		pH 5.8
    323 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    33 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    C4
    • Sodium Potassium phosphate 1.2 M pH 5.8
    		pH 5.8
    272 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    127 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    600 μl of water (100 v/v)
    C5
    • MES 0.1 M pH 5.8
    • Ammonium sulfate 1.4 M -
    		pH 5.8
    40 μl of MES pH 6.5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    59 μl of MES pH 5 (1 M)
    550 μl of water (100 v/v)
    C6
    • Ammonium di-hydrogen phosphate 1.6 M pH 5.8
    		pH 5.8
    518 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    53 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    C7
    • Sodium Potassium phosphate 1.8 M pH 5.8
    		pH 5.8
    408 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    191 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    400 μl of water (100 v/v)
    C8
    • MES 0.1 M pH 5.8
    • Ammonium sulfate 2 M -
    		pH 5.8
    40 μl of MES pH 6.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    59 μl of MES pH 5 (1 M)
    400 μl of water (100 v/v)
    C9
    • Ammonium di-hydrogen phosphate 2.2 M pH 5.8
    		pH 5.8
    712 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    73 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    C10
    • Sodium Potassium phosphate 2.4 M pH 5.8
    		pH 5.8
    544 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    255 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    200 μl of water (100 v/v)
    C11
    • MES 0.1 M pH 5.8
    • Ammonium sulfate 2.6 M -
    		pH 5.8
    40 μl of MES pH 6.5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    59 μl of MES pH 5 (1 M)
    250 μl of water (100 v/v)
    C12
    • Ammonium di-hydrogen phosphate 2.8 M pH 5.8
    		pH 5.8
    906 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    93 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    D1
    • Sodium Potassium phosphate 0.6 M pH 6.4
    		pH 6.4
    178 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    22 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    800 μl of water (100 v/v)
    D2
    • MES 0.1 M pH 6.4
    • Ammonium sulfate 0.8 M -
    		pH 6.4
    92 μl of MES pH 6.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    7 μl of MES pH 5 (1 M)
    700 μl of water (100 v/v)
    D3
    • Ammonium di-hydrogen phosphate 1 M pH 6.4
    		pH 6.4
    232 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    124 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    D4
    • Sodium Potassium phosphate 1.2 M pH 6.4
    		pH 6.4
    356 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    44 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    600 μl of water (100 v/v)
    D5
    • MES 0.1 M pH 6.4
    • Ammonium sulfate 1.4 M -
    		pH 6.4
    92 μl of MES pH 6.5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    7 μl of MES pH 5 (1 M)
    550 μl of water (100 v/v)
    D6
    • Ammonium di-hydrogen phosphate 1.6 M pH 6.4
    		pH 6.4
    371 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    199 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    D7
    • Sodium Potassium phosphate 1.8 M pH 6.4
    		pH 6.4
    534 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    66 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    400 μl of water (100 v/v)
    D8
    • MES 0.1 M pH 6.4
    • Ammonium sulfate 2 M -
    		pH 6.4
    92 μl of MES pH 6.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    7 μl of MES pH 5 (1 M)
    400 μl of water (100 v/v)
    D9
    • Ammonium di-hydrogen phosphate 2.2 M pH 6.4
    		pH 6.4
    511 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    274 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    D10
    • Sodium Potassium phosphate 2.4 M pH 6.4
    		pH 6.4
    712 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    87 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    200 μl of water (100 v/v)
    D11
    • MES 0.1 M pH 6.4
    • Ammonium sulfate 2.6 M -
    		pH 6.4
    92 μl of MES pH 6.5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    7 μl of MES pH 5 (1 M)
    250 μl of water (100 v/v)
    D12
    • Ammonium di-hydrogen phosphate 2.8 M pH 6.4
    		pH 6.4
    650 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    349 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    E1
    • Sodium Potassium phosphate 0.6 M pH 7
    		pH 7
    192 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    7 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    800 μl of water (100 v/v)
    E2
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 0.8 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    E3
    • Ammonium di-hydrogen phosphate 1 M pH 7
    		pH 7
    357 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    E4
    • Sodium Potassium phosphate 1.2 M pH 7
    		pH 7
    385 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    15 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    600 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1.4 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    E6
    • Ammonium di-hydrogen phosphate 1.6 M pH 7
    		pH 7
    571 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    E7
    • Sodium Potassium phosphate 1.8 M pH 7
    		pH 7
    577 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    22 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    400 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    E9
    • Ammonium di-hydrogen phosphate 2.2 M pH 7
    		pH 7
    785 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    E10
    • Sodium Potassium phosphate 2.4 M pH 7
    		pH 7
    770 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    30 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    200 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2.6 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    250 μl of water (100 v/v)
    E12
    • Ammonium di-hydrogen phosphate 2.8 M pH 7
    		pH 7
    1000 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    F1
    • Sodium Potassium phosphate 0.6 M pH 7.6
    		pH 7.6
    2 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    197 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    800 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.6
    • Ammonium sulfate 0.8 M -
    		pH 7.6
    77 μl of HEPES pH 7.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    F3
    • Ammonium di-hydrogen phosphate 1 M pH 7.6
    		pH 7.6
    199 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    157 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    F4
    • Sodium Potassium phosphate 1.2 M pH 7.6
    		pH 7.6
    4 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    395 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    600 μl of water (100 v/v)
    F5
    • HEPES 0.1 M pH 7.6
    • Ammonium sulfate 1.4 M -
    		pH 7.6
    77 μl of HEPES pH 7.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    350 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    F6
    • Ammonium di-hydrogen phosphate 1.6 M pH 7.6
    		pH 7.6
    318 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    252 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    F7
    • Sodium Potassium phosphate 1.8 M pH 7.6
    		pH 7.6
    6 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    593 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    400 μl of water (100 v/v)
    F8
    • HEPES 0.1 M pH 7.6
    • Ammonium sulfate 2 M -
    		pH 7.6
    77 μl of HEPES pH 7.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    F9
    • Ammonium di-hydrogen phosphate 2.2 M pH 7.6
    		pH 7.6
    438 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    347 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    F10
    • Sodium Potassium phosphate 2.4 M pH 7.6
    		pH 7.6
    8 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    791 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    200 μl of water (100 v/v)
    F11
    • HEPES 0.1 M pH 7.6
    • Ammonium sulfate 2.6 M -
    		pH 7.6
    77 μl of HEPES pH 7.5 (1 M)
    22 μl of HEPES pH 8 (1 M)
    650 μl of Ammonium sulfate (4 M)
    250 μl of water (100 v/v)
    F12
    • Ammonium di-hydrogen phosphate 2.8 M pH 7.6
    		pH 7.6
    557 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    442 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    G1
    • Sodium Potassium phosphate 0.6 M pH 8.2
    		pH 8.2
    0 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    199 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    800 μl of water (100 v/v)
    G2
    • tris chloride BCC 0.1 M pH 8.2
    • Ammonium sulfate 0.8 M -
    		pH 8.2
    57 μl of tris chloride BCC pH 8 (1 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    700 μl of water (100 v/v)
    G3
    • Ammonium di-hydrogen phosphate 1 M pH 8.2
    		pH 8.2
    316 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    40 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    642 μl of water (100 v/v)
    G4
    • Sodium Potassium phosphate 1.2 M pH 8.2
    		pH 8.2
    1 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    398 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    600 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 8.2
    • Ammonium sulfate 1.4 M -
    		pH 8.2
    57 μl of tris chloride BCC pH 8 (1 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    550 μl of water (100 v/v)
    G6
    • Ammonium di-hydrogen phosphate 1.6 M pH 8.2
    		pH 8.2
    507 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    64 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    428 μl of water (100 v/v)
    G7
    • Sodium Potassium phosphate 1.8 M pH 8.2
    		pH 8.2
    2 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    597 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    400 μl of water (100 v/v)
    G8
    • tris chloride BCC 0.1 M pH 8.2
    • Ammonium sulfate 2 M -
    		pH 8.2
    57 μl of tris chloride BCC pH 8 (1 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    G9
    • Ammonium di-hydrogen phosphate 2.2 M pH 8.2
    		pH 8.2
    697 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    88 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    214 μl of water (100 v/v)
    G10
    • Sodium Potassium phosphate 2.4 M pH 8.2
    		pH 8.2
    2 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    797 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    200 μl of water (100 v/v)
    G11
    • tris chloride BCC 0.1 M pH 8.2
    • Ammonium sulfate 2.6 M -
    		pH 8.2
    57 μl of tris chloride BCC pH 8 (1 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    250 μl of water (100 v/v)
    G12
    • Ammonium di-hydrogen phosphate 2.8 M pH 8.2
    		pH 8.2
    887 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    112 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    H1
    • Sodium Potassium phosphate 0.6 M pH 8.8
    		pH 8.8
    200 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    800 μl of water (100 v/v)
    H2
    • tris chloride BCC 0.1 M pH 8.8
    • Ammonium sulfate 0.8 M -
    		pH 8.8
    31 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium sulfate (4 M)
    68 μl of tris chloride BCC pH 9 (1 M)
    700 μl of water (100 v/v)
    H3
    • Ammonium di-hydrogen phosphate 1 M pH 8.8
    		pH 8.8
    357 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    642 μl of water (100 v/v)
    H4
    • Sodium Potassium phosphate 1.2 M pH 8.8
    		pH 8.8
    400 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    600 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8.8
    • Ammonium sulfate 1.4 M -
    		pH 8.8
    31 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of Ammonium sulfate (4 M)
    68 μl of tris chloride BCC pH 9 (1 M)
    550 μl of water (100 v/v)
    H6
    • Ammonium di-hydrogen phosphate 1.6 M pH 8.8
    		pH 8.8
    571 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    428 μl of water (100 v/v)
    H7
    • Sodium Potassium phosphate 1.8 M pH 8.8
    		pH 8.8
    600 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    400 μl of water (100 v/v)
    H8
    • tris chloride BCC 0.1 M pH 8.8
    • Ammonium sulfate 2 M -
    		pH 8.8
    31 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (4 M)
    68 μl of tris chloride BCC pH 9 (1 M)
    400 μl of water (100 v/v)
    H9
    • Ammonium di-hydrogen phosphate 2.2 M pH 8.8
    		pH 8.8
    785 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
    214 μl of water (100 v/v)
    H10
    • Sodium Potassium phosphate 2.4 M pH 8.8
    		pH 8.8
    800 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    200 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8.8
    • Ammonium sulfate 2.6 M -
    		pH 8.8
    31 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of Ammonium sulfate (4 M)
    68 μl of tris chloride BCC pH 9 (1 M)
    250 μl of water (100 v/v)
    H12
    • Ammonium di-hydrogen phosphate 2.8 M pH 8.8
    		pH 8.8
    1000 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)