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Supplemental Information

Screen Description

Cubic Phase I

Qiagen / Cubic Phase I
Created 2009-11-16.

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Cubic Phase I

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Cubic Phase I

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Screen Structure

Cubic Phase I

Well Count 96
Number of distinct ingredients 7
Internal diversity (score mean dist) 0.789

Screen Composition

More information about screen composition chart.
2025-08-11T20:31:47.116464 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Cubic Phase I
Buffer 96
Salt 104

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Cubic Phase I Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium Potassium phosphate 0.6 M pH 4.6
pH 4.6
200.0 μl of Sodium Potassium phosphate pH 4.6 (3 M)
800.0 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.8 M -
pH 4.6
200.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
A3
  • Ammonium di-hydrogen phosphate 1 M pH 4.6
pH 4.6
357.1 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
642.9 μl of water (100 v/v)
A4
  • Sodium Potassium phosphate 1.2 M pH 4.6
pH 4.6
400.0 μl of Sodium Potassium phosphate pH 4.6 (3 M)
600.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 1.4 M -
pH 4.6
350.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
A6
  • Ammonium di-hydrogen phosphate 1.6 M pH 4.6
pH 4.6
571.4 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
428.6 μl of water (100 v/v)
A7
  • Sodium Potassium phosphate 1.8 M pH 4.6
pH 4.6
600.0 μl of Sodium Potassium phosphate pH 4.6 (3 M)
400.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
A9
  • Ammonium di-hydrogen phosphate 2.8 M pH 4.6
pH 4.6
1000.0 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
A10
  • Sodium Potassium phosphate 2.4 M pH 4.6
pH 4.6
800.0 μl of Sodium Potassium phosphate pH 4.6 (3 M)
200.0 μl of water (100 v/v)
A11
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2.6 M -
pH 4.6
650.0 μl of Ammonium sulfate (4 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
250.0 μl of water (100 v/v)
A12
  • Ammonium di-hydrogen phosphate 2.8 M pH 4.6
pH 4.6
1000.0 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
B1
  • Sodium Potassium phosphate 0.6 M pH 5.2
pH 5.2
162.9 μl of Sodium Potassium phosphate pH 4.6 (3 M)
37.1 μl of Sodium Potassium phosphate pH 6.2 (3 M)
800.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 5.2
  • Ammonium sulfate 0.8 M -
pH 5.2
200.0 μl of Ammonium sulfate (4 M)
59.2 μl of Sodium acetate pH 5 (1 M)
40.8 μl of Sodium acetate pH 5.6 (1 M)
700.0 μl of water (100 v/v)
B3
  • Ammonium di-hydrogen phosphate 1 M pH 5.2
pH 5.2
350.3 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
6.9 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
642.9 μl of water (100 v/v)
B4
  • Sodium Potassium phosphate 1.2 M pH 5.2
pH 5.2
325.9 μl of Sodium Potassium phosphate pH 4.6 (3 M)
74.1 μl of Sodium Potassium phosphate pH 6.2 (3 M)
600.0 μl of water (100 v/v)
B5
  • Sodium acetate 0.1 M pH 5.2
  • Ammonium sulfate 1.4 M -
pH 5.2
350.0 μl of Ammonium sulfate (4 M)
59.2 μl of Sodium acetate pH 5 (1 M)
40.8 μl of Sodium acetate pH 5.6 (1 M)
550.0 μl of water (100 v/v)
B6
  • Ammonium di-hydrogen phosphate 1.6 M pH 5.2
pH 5.2
560.4 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
11.0 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
428.6 μl of water (100 v/v)
B7
  • Sodium Potassium phosphate 1.8 M pH 5.2
pH 5.2
488.8 μl of Sodium Potassium phosphate pH 4.6 (3 M)
111.2 μl of Sodium Potassium phosphate pH 6.2 (3 M)
400.0 μl of water (100 v/v)
B8
  • Sodium acetate 0.1 M pH 5.2
  • Ammonium sulfate 2 M -
pH 5.2
500.0 μl of Ammonium sulfate (4 M)
59.2 μl of Sodium acetate pH 5 (1 M)
40.8 μl of Sodium acetate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
B9
  • Ammonium di-hydrogen phosphate 2.2 M pH 5.2
pH 5.2
770.6 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
15.1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
214.3 μl of water (100 v/v)
B10
  • Sodium Potassium phosphate 2.4 M pH 5.2
pH 5.2
651.8 μl of Sodium Potassium phosphate pH 4.6 (3 M)
148.2 μl of Sodium Potassium phosphate pH 6.2 (3 M)
200.0 μl of water (100 v/v)
B11
  • Sodium acetate 0.1 M pH 5.2
  • Ammonium sulfate 2.6 M -
pH 5.2
650.0 μl of Ammonium sulfate (4 M)
59.2 μl of Sodium acetate pH 5 (1 M)
40.8 μl of Sodium acetate pH 5.6 (1 M)
250.0 μl of water (100 v/v)
B12
  • Ammonium di-hydrogen phosphate 2.8 M pH 5.2
pH 5.2
980.8 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
19.2 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
C1
  • Sodium Potassium phosphate 0.6 M pH 5.8
pH 5.8
152.4 μl of Sodium Potassium phosphate pH 4.6 (3 M)
47.6 μl of Sodium Potassium phosphate pH 6.2 (3 M)
800.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 5.8
  • Ammonium sulfate 0.8 M -
pH 5.8
200.0 μl of Ammonium sulfate (4 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
700.0 μl of water (100 v/v)
C3
  • Ammonium di-hydrogen phosphate 1 M pH 5.8
pH 5.8
323.9 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
33.2 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
642.9 μl of water (100 v/v)
C4
  • Sodium Potassium phosphate 1.2 M pH 5.8
pH 5.8
304.7 μl of Sodium Potassium phosphate pH 4.6 (3 M)
95.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
600.0 μl of water (100 v/v)
C5
  • MES 0.1 M pH 5.8
  • Ammonium sulfate 1.4 M -
pH 5.8
350.0 μl of Ammonium sulfate (4 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
550.0 μl of water (100 v/v)
C6
  • Ammonium di-hydrogen phosphate 1.6 M pH 5.8
pH 5.8
518.2 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
53.2 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
428.6 μl of water (100 v/v)
C7
  • Sodium Potassium phosphate 1.8 M pH 5.8
pH 5.8
457.1 μl of Sodium Potassium phosphate pH 4.6 (3 M)
142.9 μl of Sodium Potassium phosphate pH 6.2 (3 M)
400.0 μl of water (100 v/v)
C8
  • MES 0.1 M pH 5.8
  • Ammonium sulfate 2 M -
pH 5.8
500.0 μl of Ammonium sulfate (4 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
400.0 μl of water (100 v/v)
C9
  • Ammonium di-hydrogen phosphate 2.2 M pH 5.8
pH 5.8
712.6 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
73.1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
214.3 μl of water (100 v/v)
C10
  • Sodium Potassium phosphate 2.4 M pH 5.8
pH 5.8
609.4 μl of Sodium Potassium phosphate pH 4.6 (3 M)
190.6 μl of Sodium Potassium phosphate pH 6.2 (3 M)
200.0 μl of water (100 v/v)
C11
  • MES 0.1 M pH 5.8
  • Ammonium sulfate 2.6 M -
pH 5.8
650.0 μl of Ammonium sulfate (4 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
250.0 μl of water (100 v/v)
C12
  • Ammonium di-hydrogen phosphate 2.8 M pH 5.8
pH 5.8
906.9 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
93.1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
D1
  • Sodium Potassium phosphate 0.6 M pH 6.4
pH 6.4
104.7 μl of Sodium Potassium phosphate pH 6.2 (3 M)
95.3 μl of Sodium Potassium phosphate pH 7.5 (3 M)
800.0 μl of water (100 v/v)
D2
  • MES 0.1 M pH 6.4
  • Ammonium sulfate 0.8 M -
pH 6.4
200.0 μl of Ammonium sulfate (4 M)
92.3 μl of MES pH 6.5 (1 M)
7.7 μl of MES pH 5 (1 M)
700.0 μl of water (100 v/v)
D3
  • Ammonium di-hydrogen phosphate 1 M pH 6.4
pH 6.4
232.4 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
124.8 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
642.9 μl of water (100 v/v)
D4
  • Sodium Potassium phosphate 1.2 M pH 6.4
pH 6.4
209.5 μl of Sodium Potassium phosphate pH 6.2 (3 M)
190.5 μl of Sodium Potassium phosphate pH 7.5 (3 M)
600.0 μl of water (100 v/v)
D5
  • MES 0.1 M pH 6.4
  • Ammonium sulfate 1.4 M -
pH 6.4
350.0 μl of Ammonium sulfate (4 M)
92.3 μl of MES pH 6.5 (1 M)
7.7 μl of MES pH 5 (1 M)
550.0 μl of water (100 v/v)
D6
  • Ammonium di-hydrogen phosphate 1.6 M pH 6.4
pH 6.4
371.8 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
199.7 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
428.6 μl of water (100 v/v)
D7
  • Sodium Potassium phosphate 1.8 M pH 6.4
pH 6.4
314.2 μl of Sodium Potassium phosphate pH 6.2 (3 M)
285.8 μl of Sodium Potassium phosphate pH 7.5 (3 M)
400.0 μl of water (100 v/v)
D8
  • MES 0.1 M pH 6.4
  • Ammonium sulfate 2 M -
pH 6.4
500.0 μl of Ammonium sulfate (4 M)
92.3 μl of MES pH 6.5 (1 M)
7.7 μl of MES pH 5 (1 M)
400.0 μl of water (100 v/v)
D9
  • Ammonium di-hydrogen phosphate 2.2 M pH 6.4
pH 6.4
511.2 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
274.5 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
214.3 μl of water (100 v/v)
D10
  • Sodium Potassium phosphate 2.4 M pH 6.4
pH 6.4
418.9 μl of Sodium Potassium phosphate pH 6.2 (3 M)
381.1 μl of Sodium Potassium phosphate pH 7.5 (3 M)
200.0 μl of water (100 v/v)
D11
  • MES 0.1 M pH 6.4
  • Ammonium sulfate 2.6 M -
pH 6.4
650.0 μl of Ammonium sulfate (4 M)
92.3 μl of MES pH 6.5 (1 M)
7.7 μl of MES pH 5 (1 M)
250.0 μl of water (100 v/v)
D12
  • Ammonium di-hydrogen phosphate 2.8 M pH 6.4
pH 6.4
650.6 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
349.4 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
E1
  • Sodium Potassium phosphate 0.6 M pH 7
pH 7
54.5 μl of Sodium Potassium phosphate pH 6.2 (3 M)
145.5 μl of Sodium Potassium phosphate pH 7.5 (3 M)
800.0 μl of water (100 v/v)
E2
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 0.8 M -
pH 7
200.0 μl of Ammonium sulfate (4 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
E3
  • Ammonium di-hydrogen phosphate 1 M pH 7
pH 7
357.1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
642.9 μl of water (100 v/v)
E4
  • Sodium Potassium phosphate 1.2 M pH 7
pH 7
109.0 μl of Sodium Potassium phosphate pH 6.2 (3 M)
291.0 μl of Sodium Potassium phosphate pH 7.5 (3 M)
600.0 μl of water (100 v/v)
E5
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 1.4 M -
pH 7
350.0 μl of Ammonium sulfate (4 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
550.0 μl of water (100 v/v)
E6
  • Ammonium di-hydrogen phosphate 1.6 M pH 7
pH 7
571.4 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
428.6 μl of water (100 v/v)
E7
  • Sodium Potassium phosphate 1.8 M pH 7
pH 7
163.6 μl of Sodium Potassium phosphate pH 6.2 (3 M)
436.4 μl of Sodium Potassium phosphate pH 7.5 (3 M)
400.0 μl of water (100 v/v)
E8
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 2 M -
pH 7
500.0 μl of Ammonium sulfate (4 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
400.0 μl of water (100 v/v)
E9
  • Ammonium di-hydrogen phosphate 2.2 M pH 7
pH 7
785.7 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
214.3 μl of water (100 v/v)
E10
  • Sodium Potassium phosphate 2.4 M pH 7
pH 7
218.1 μl of Sodium Potassium phosphate pH 6.2 (3 M)
581.9 μl of Sodium Potassium phosphate pH 7.5 (3 M)
200.0 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7
  • Ammonium sulfate 2.6 M -
pH 7
650.0 μl of Ammonium sulfate (4 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
E12
  • Ammonium di-hydrogen phosphate 2.8 M pH 7
pH 7
1000.0 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
F1
  • Sodium Potassium phosphate 0.6 M pH 7.6
pH 7.6
180.2 μl of Sodium Potassium phosphate pH 8.8 (3 M)
19.8 μl of Sodium Potassium phosphate pH 7.5 (3 M)
800.0 μl of water (100 v/v)
F2
  • HEPES 0.1 M pH 7.6
  • Ammonium sulfate 0.8 M -
pH 7.6
200.0 μl of Ammonium sulfate (4 M)
77.9 μl of HEPES pH 7.5 (1 M)
22.1 μl of HEPES pH 8 (1 M)
700.0 μl of water (100 v/v)
F3
  • Ammonium di-hydrogen phosphate 1 M pH 7.6
pH 7.6
157.9 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
199.2 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
642.9 μl of water (100 v/v)
F4
  • Sodium Potassium phosphate 1.2 M pH 7.6
pH 7.6
360.4 μl of Sodium Potassium phosphate pH 8.8 (3 M)
39.6 μl of Sodium Potassium phosphate pH 7.5 (3 M)
600.0 μl of water (100 v/v)
F5
  • HEPES 0.1 M pH 7.6
  • Ammonium sulfate 1.4 M -
pH 7.6
350.0 μl of Ammonium sulfate (4 M)
77.9 μl of HEPES pH 7.5 (1 M)
22.1 μl of HEPES pH 8 (1 M)
550.0 μl of water (100 v/v)
F6
  • Ammonium di-hydrogen phosphate 1.6 M pH 7.6
pH 7.6
252.6 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
318.8 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
428.6 μl of water (100 v/v)
F7
  • Sodium Potassium phosphate 1.8 M pH 7.6
pH 7.6
540.6 μl of Sodium Potassium phosphate pH 8.8 (3 M)
59.4 μl of Sodium Potassium phosphate pH 7.5 (3 M)
400.0 μl of water (100 v/v)
F8
  • HEPES 0.1 M pH 7.6
  • Ammonium sulfate 2 M -
pH 7.6
500.0 μl of Ammonium sulfate (4 M)
77.9 μl of HEPES pH 7.5 (1 M)
22.1 μl of HEPES pH 8 (1 M)
400.0 μl of water (100 v/v)
F9
  • Ammonium di-hydrogen phosphate 2.2 M pH 7.6
pH 7.6
347.4 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
438.3 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
214.3 μl of water (100 v/v)
F10
  • Sodium Potassium phosphate 2.4 M pH 7.6
pH 7.6
720.8 μl of Sodium Potassium phosphate pH 8.8 (3 M)
79.2 μl of Sodium Potassium phosphate pH 7.5 (3 M)
200.0 μl of water (100 v/v)
F11
  • HEPES 0.1 M pH 7.6
  • Ammonium sulfate 2.6 M -
pH 7.6
650.0 μl of Ammonium sulfate (4 M)
77.9 μl of HEPES pH 7.5 (1 M)
22.1 μl of HEPES pH 8 (1 M)
250.0 μl of water (100 v/v)
F12
  • Ammonium di-hydrogen phosphate 2.8 M pH 7.6
pH 7.6
442.1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
557.9 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
G1
  • Sodium Potassium phosphate 0.6 M pH 8.2
pH 8.2
193.2 μl of Sodium Potassium phosphate pH 8.8 (3 M)
6.8 μl of Sodium Potassium phosphate pH 7.5 (3 M)
800.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.1 M pH 8.2
  • Ammonium sulfate 0.8 M -
pH 8.2
200.0 μl of Ammonium sulfate (4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
G3
  • Ammonium di-hydrogen phosphate 1 M pH 8.2
pH 8.2
40.3 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
316.9 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
642.9 μl of water (100 v/v)
G4
  • Sodium Potassium phosphate 1.2 M pH 8.2
pH 8.2
386.5 μl of Sodium Potassium phosphate pH 8.8 (3 M)
13.5 μl of Sodium Potassium phosphate pH 7.5 (3 M)
600.0 μl of water (100 v/v)
G5
  • tris chloride BCC 0.1 M pH 8.2
  • Ammonium sulfate 1.4 M -
pH 8.2
350.0 μl of Ammonium sulfate (4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G6
  • Ammonium di-hydrogen phosphate 1.6 M pH 8.2
pH 8.2
64.5 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
507.0 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
428.6 μl of water (100 v/v)
G7
  • Sodium Potassium phosphate 1.8 M pH 8.2
pH 8.2
579.7 μl of Sodium Potassium phosphate pH 8.8 (3 M)
20.3 μl of Sodium Potassium phosphate pH 7.5 (3 M)
400.0 μl of water (100 v/v)
G8
  • tris chloride BCC 0.1 M pH 8.2
  • Ammonium sulfate 2 M -
pH 8.2
500.0 μl of Ammonium sulfate (4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
G9
  • Ammonium di-hydrogen phosphate 2.2 M pH 8.2
pH 8.2
88.6 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
697.1 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
214.3 μl of water (100 v/v)
G10
  • Sodium Potassium phosphate 2.4 M pH 8.2
pH 8.2
773.0 μl of Sodium Potassium phosphate pH 8.8 (3 M)
27.0 μl of Sodium Potassium phosphate pH 7.5 (3 M)
200.0 μl of water (100 v/v)
G11
  • tris chloride BCC 0.1 M pH 8.2
  • Ammonium sulfate 2.6 M -
pH 8.2
650.0 μl of Ammonium sulfate (4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
250.0 μl of water (100 v/v)
G12
  • Ammonium di-hydrogen phosphate 2.8 M pH 8.2
pH 8.2
112.8 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
887.2 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
H1
  • Sodium Potassium phosphate 0.6 M pH 8.8
pH 8.8
200.0 μl of Sodium Potassium phosphate pH 8.8 (3 M)
800.0 μl of water (100 v/v)
H2
  • tris chloride BCC 0.1 M pH 8.8
  • Ammonium sulfate 0.8 M -
pH 8.8
200.0 μl of Ammonium sulfate (4 M)
31.5 μl of tris chloride BCC pH 8.5 (1 M)
68.5 μl of tris chloride BCC pH 9 (1 M)
700.0 μl of water (100 v/v)
H3
  • Ammonium di-hydrogen phosphate 1 M pH 8.8
pH 8.8
357.1 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
642.9 μl of water (100 v/v)
H4
  • Sodium Potassium phosphate 1.2 M pH 8.8
pH 8.8
400.0 μl of Sodium Potassium phosphate pH 8.8 (3 M)
600.0 μl of water (100 v/v)
H5
  • tris chloride BCC 0.1 M pH 8.8
  • Ammonium sulfate 1.4 M -
pH 8.8
350.0 μl of Ammonium sulfate (4 M)
31.5 μl of tris chloride BCC pH 8.5 (1 M)
68.5 μl of tris chloride BCC pH 9 (1 M)
550.0 μl of water (100 v/v)
H6
  • Ammonium di-hydrogen phosphate 1.6 M pH 8.8
pH 8.8
571.4 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
428.6 μl of water (100 v/v)
H7
  • Sodium Potassium phosphate 1.8 M pH 8.8
pH 8.8
600.0 μl of Sodium Potassium phosphate pH 8.8 (3 M)
400.0 μl of water (100 v/v)
H8
  • tris chloride BCC 0.1 M pH 8.8
  • Ammonium sulfate 2 M -
pH 8.8
500.0 μl of Ammonium sulfate (4 M)
31.5 μl of tris chloride BCC pH 8.5 (1 M)
68.5 μl of tris chloride BCC pH 9 (1 M)
400.0 μl of water (100 v/v)
H9
  • Ammonium di-hydrogen phosphate 2.2 M pH 8.8
pH 8.8
785.7 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)
214.3 μl of water (100 v/v)
H10
  • Sodium Potassium phosphate 2.4 M pH 8.8
pH 8.8
800.0 μl of Sodium Potassium phosphate pH 8.8 (3 M)
200.0 μl of water (100 v/v)
H11
  • tris chloride BCC 0.1 M pH 8.8
  • Ammonium sulfate 2.6 M -
pH 8.8
650.0 μl of Ammonium sulfate (4 M)
31.5 μl of tris chloride BCC pH 8.5 (1 M)
68.5 μl of tris chloride BCC pH 9 (1 M)
250.0 μl of water (100 v/v)
H12
  • Ammonium di-hydrogen phosphate 2.8 M pH 8.8
pH 8.8
1000.0 μl of Ammonium di-hydrogen phosphate pH 8.8 (2.8 M)