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Supplemental Information

Screen Description

Cubic Phase II

Qiagen / Cubic Phase II
Created 2009-11-16.

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Cubic Phase II

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Screen Structure

Cubic Phase II

Well Count 96
Number of distinct ingredients 12
Internal diversity (score mean dist) 0.841

Screen Composition

2025-08-16T09:05:43.916736 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Cubic Phase II
Buffer 128
Polymer 96
Salt 120

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Cubic Phase II Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • Sodium chloride 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
A2
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
740.0 μl of water (100 v/v)
A3
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
A4
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • Sodium chloride 2 M -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
400.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
A5
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • Sodium chloride 0.2 M -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
40.0 μl of Sodium chloride (5 M)
740.0 μl of water (100 v/v)
A6
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
700.0 μl of water (100 v/v)
A7
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • Sodium chloride 2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
400.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
A8
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
40.0 μl of Sodium chloride (5 M)
740.0 μl of water (100 v/v)
A9
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
700.0 μl of water (100 v/v)
A10
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • Sodium chloride 2 M -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
400.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
460.0 μl of water (100 v/v)
A11
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
40.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
740.0 μl of water (100 v/v)
A12
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
B1
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • di-Sodium malonate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
588.2 μl of di-Sodium malonate (3.4 M)
271.8 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • di-Sodium malonate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
58.8 μl of di-Sodium malonate (3.4 M)
721.2 μl of water (100 v/v)
B3
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 0.01 M -
  • Calcium chloride 0.01 M -
pH 4.6
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
691.0 μl of water (100 v/v)
B4
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • di-Sodium malonate 2 M -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
588.2 μl of di-Sodium malonate (3.4 M)
271.8 μl of water (100 v/v)
B5
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • di-Sodium malonate 0.2 M -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
58.8 μl of di-Sodium malonate (3.4 M)
721.2 μl of water (100 v/v)
B6
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • MES 0.1 M pH 5.8
  • Magnesium chloride 0.01 M -
  • Calcium chloride 0.01 M -
pH 5.8
5.0 μl of Calcium chloride (2 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
691.0 μl of water (100 v/v)
B7
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • di-Sodium malonate 2 M -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
588.2 μl of di-Sodium malonate (3.4 M)
271.8 μl of water (100 v/v)
B8
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • di-Sodium malonate 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
58.8 μl of di-Sodium malonate (3.4 M)
721.2 μl of water (100 v/v)
B9
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • HEPES 0.1 M pH 7
  • Magnesium chloride 0.01 M -
  • Calcium chloride 0.01 M -
pH 7
5.0 μl of Calcium chloride (2 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
691.0 μl of water (100 v/v)
B10
  • Polyethylene glycol monomethyl ether 550 4 v/v -
  • di-Sodium malonate 2 M -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
40.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
588.2 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
271.8 μl of water (100 v/v)
B11
  • Polyethylene glycol monomethyl ether 550 12 v/v -
  • di-Sodium malonate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
120.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
58.8 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
721.2 μl of water (100 v/v)
B12
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 8.2
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
691.0 μl of water (100 v/v)
C1
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
C2
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
C3
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
C4
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
C5
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
C6
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
C7
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
C8
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
C9
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
C10
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 2 M -
pH 8.2
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
420.0 μl of water (100 v/v)
C11
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
620.0 μl of water (100 v/v)
C12
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
D1
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 2 M -
pH 4.6
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
588.2 μl of di-Sodium malonate (3.4 M)
231.8 μl of water (100 v/v)
D2
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
D3
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 4.6
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
491.0 μl of water (100 v/v)
D4
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
588.2 μl of di-Sodium malonate (3.4 M)
231.8 μl of water (100 v/v)
D5
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
D6
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • MES 0.1 M pH 5.8
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 5.8
5.0 μl of Calcium chloride (2 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
491.0 μl of water (100 v/v)
D7
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 0.1 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
29.4 μl of di-Sodium malonate (3.4 M)
790.6 μl of water (100 v/v)
D8
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 0.1 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
29.4 μl of di-Sodium malonate (3.4 M)
630.6 μl of water (100 v/v)
D9
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 7
5.0 μl of Calcium chloride (2 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
491.0 μl of water (100 v/v)
D10
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 2 M -
pH 8.2
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
588.2 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
231.8 μl of water (100 v/v)
D11
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
601.2 μl of water (100 v/v)
D12
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 8.2
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
491.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 3,350 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 3,350 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 3,350 4 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 3,350 12 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 3,350 20 w/v -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 3,350 4 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 3,350 12 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 3,350 20 w/v -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 3,350 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 2 M -
pH 8.2
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
420.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 3,350 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
620.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 3,350 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 2 M -
pH 4.6
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
588.2 μl of di-Sodium malonate (3.4 M)
231.8 μl of water (100 v/v)
F2
  • Polyethylene glycol 3,350 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 2 M -
pH 4.6
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
588.2 μl of di-Sodium malonate (3.4 M)
71.8 μl of water (100 v/v)
F3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 4.6
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
491.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 3,350 4 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 1 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
294.1 μl of di-Sodium malonate (3.4 M)
525.9 μl of water (100 v/v)
F5
  • Polyethylene glycol 3,350 12 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
F6
  • Polyethylene glycol 3,350 20 w/v -
  • MES 0.1 M pH 5.8
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 5.8
5.0 μl of Calcium chloride (2 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
491.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 3,350 4 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 1 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
294.1 μl of di-Sodium malonate (3.4 M)
525.9 μl of water (100 v/v)
F8
  • Polyethylene glycol 3,350 12 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 0.2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
F9
  • Polyethylene glycol 3,350 20 w/v -
  • HEPES 0.1 M pH 7
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 7
5.0 μl of Calcium chloride (2 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
491.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 3,350 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 1 M -
pH 8.2
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
294.1 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
525.9 μl of water (100 v/v)
F11
  • Polyethylene glycol 3,350 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
601.2 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,350 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 8.2
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
491.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 6,000 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 6,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 6,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 6,000 4 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 6,000 12 w/v -
  • MES 0.1 M pH 5.8
  • Sodium chloride 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 5.8
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 6,000 4 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 6,000 12 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 6,000 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 2 M -
pH 8.2
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
420.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 6,000 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Sodium chloride 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
620.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
pH 8.2
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 6,000 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 1 M -
pH 4.6
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
294.1 μl of di-Sodium malonate (3.4 M)
525.9 μl of water (100 v/v)
H2
  • Polyethylene glycol 6,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
H3
  • Polyethylene glycol 6,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 4.6
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
491.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 6,000 4 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 0.5 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
147.1 μl of di-Sodium malonate (3.4 M)
672.9 μl of water (100 v/v)
H5
  • Polyethylene glycol 6,000 12 w/v -
  • MES 0.1 M pH 5.8
  • di-Sodium malonate 0.2 M -
pH 5.8
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
H6
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 5.8
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 5.8
5.0 μl of Calcium chloride (2 M)
40.6 μl of MES pH 6.5 (1 M)
59.4 μl of MES pH 5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
491.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 6,000 4 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 0.5 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
147.1 μl of di-Sodium malonate (3.4 M)
672.9 μl of water (100 v/v)
H8
  • Polyethylene glycol 6,000 12 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium malonate 0.2 M -
pH 7
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
601.2 μl of water (100 v/v)
H9
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 7
5.0 μl of Calcium chloride (2 M)
36.5 μl of HEPES pH 7.5 (1 M)
63.5 μl of HEPES pH 6.5 (1 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
491.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 6,000 4 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 2 M -
pH 8.2
80.0 μl of Polyethylene glycol 6,000 (50 w/v)
588.2 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
231.8 μl of water (100 v/v)
H11
  • Polyethylene glycol 6,000 12 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • di-Sodium malonate 0.2 M -
pH 8.2
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
58.8 μl of di-Sodium malonate (3.4 M)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
601.2 μl of water (100 v/v)
H12
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.2
  • Calcium chloride 0.01 M -
  • Magnesium chloride 0.01 M -
pH 8.2
5.0 μl of Calcium chloride (2 M)
4.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
42.1 μl of tris chloride BCC pH 8.5 (1 M)
57.9 μl of tris chloride BCC pH 8 (1 M)
491.0 μl of water (100 v/v)