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Supplemental Information

Screen Description

Cubic Phase II

Qiagen / Cubic Phase II
Created 2009-11-16.

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Cubic Phase II

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Cubic Phase II

    Screen Structure

    Cubic Phase II

    Well Count 96
    Number of distinct ingredients 12
    Internal diversity (score mean dist) 0.841

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:44:28.871031 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Cubic Phase II
    Buffer 128
    Polymer 96
    Salt 120

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Cubic Phase II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • Sodium chloride 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    460 μl of water (100 v/v)
    A2
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    A3
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    700 μl of water (100 v/v)
    A4
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • Sodium chloride 2 M -
    • MES 0.1 M pH 5.8
    		pH 5.8
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    A5
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • Sodium chloride 0.2 M -
    • MES 0.1 M pH 5.8
    		pH 5.8
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    740 μl of water (100 v/v)
    A6
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • MES 0.1 M pH 5.8
    		pH 5.8
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    700 μl of water (100 v/v)
    A7
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • Sodium chloride 2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    400 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    460 μl of water (100 v/v)
    A8
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    740 μl of water (100 v/v)
    A9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • HEPES 0.1 M pH 7
    		pH 7
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    A10
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • Sodium chloride 2 M -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    460 μl of water (100 v/v)
    A11
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    740 μl of water (100 v/v)
    A12
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    700 μl of water (100 v/v)
    B1
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • di-Sodium malonate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    588 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    271 μl of water (100 v/v)
    B2
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • di-Sodium malonate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    58 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    721 μl of water (100 v/v)
    B3
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium chloride 0.01 M -
    • Calcium chloride 0.01 M -
    		pH 4.6
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    5 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    691 μl of water (100 v/v)
    B4
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • di-Sodium malonate 2 M -
    • MES 0.1 M pH 5.8
    		pH 5.8
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    588 μl of di-Sodium malonate (3.4 M)
    271 μl of water (100 v/v)
    B5
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • di-Sodium malonate 0.2 M -
    • MES 0.1 M pH 5.8
    		pH 5.8
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    721 μl of water (100 v/v)
    B6
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • MES 0.1 M pH 5.8
    • Magnesium chloride 0.01 M -
    • Calcium chloride 0.01 M -
    		pH 5.8
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    59 μl of MES pH 5 (1 M)
    5 μl of Calcium chloride (2 M)
    40 μl of MES pH 6.5 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    691 μl of water (100 v/v)
    B7
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • di-Sodium malonate 2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    588 μl of di-Sodium malonate (3.4 M)
    271 μl of water (100 v/v)
    B8
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • di-Sodium malonate 0.2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    721 μl of water (100 v/v)
    B9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.01 M -
    • Calcium chloride 0.01 M -
    		pH 7
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    5 μl of Calcium chloride (2 M)
    4 μl of Magnesium chloride (2.5 M)
    691 μl of water (100 v/v)
    B10
    • Polyethylene glycol monomethyl ether 550 4 v/v -
    • di-Sodium malonate 2 M -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    40 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    588 μl of di-Sodium malonate (3.4 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    271 μl of water (100 v/v)
    B11
    • Polyethylene glycol monomethyl ether 550 12 v/v -
    • di-Sodium malonate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    120 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    721 μl of water (100 v/v)
    B12
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 8.2
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    5 μl of Calcium chloride (2 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    691 μl of water (100 v/v)
    C1
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    		pH 4.6
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    420 μl of water (100 v/v)
    C2
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 0.2 M -
    		pH 4.6
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    620 μl of water (100 v/v)
    C3
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C4
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Sodium chloride (5 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    420 μl of water (100 v/v)
    C5
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    620 μl of water (100 v/v)
    C6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • MES 0.1 M pH 5.8
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C7
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    400 μl of Sodium chloride (5 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    420 μl of water (100 v/v)
    C8
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    40 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    620 μl of water (100 v/v)
    C9
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    C10
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Sodium chloride (5 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    420 μl of water (100 v/v)
    C11
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    620 μl of water (100 v/v)
    C12
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    D1
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 2 M -
    		pH 4.6
    588 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    231 μl of water (100 v/v)
    D2
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 0.2 M -
    		pH 4.6
    58 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    601 μl of water (100 v/v)
    D3
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 4.6
    5 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    D4
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    588 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    231 μl of water (100 v/v)
    D5
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    601 μl of water (100 v/v)
    D6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • MES 0.1 M pH 5.8
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    5 μl of Calcium chloride (2 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    D7
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 0.1 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    29 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    790 μl of water (100 v/v)
    D8
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 0.1 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    29 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    630 μl of water (100 v/v)
    D9
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    D10
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    588 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    231 μl of water (100 v/v)
    D11
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    601 μl of water (100 v/v)
    D12
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 8.2
    5 μl of Calcium chloride (2 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,350 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    		pH 4.6
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    420 μl of water (100 v/v)
    E2
    • Polyethylene glycol 3,350 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 0.2 M -
    		pH 4.6
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    620 μl of water (100 v/v)
    E3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    E4
    • Polyethylene glycol 3,350 4 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    E5
    • Polyethylene glycol 3,350 12 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    620 μl of water (100 v/v)
    E6
    • Polyethylene glycol 3,350 20 w/v -
    • MES 0.1 M pH 5.8
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,350 4 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    E8
    • Polyethylene glycol 3,350 12 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    620 μl of water (100 v/v)
    E9
    • Polyethylene glycol 3,350 20 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E10
    • Polyethylene glycol 3,350 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    420 μl of water (100 v/v)
    E11
    • Polyethylene glycol 3,350 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    620 μl of water (100 v/v)
    E12
    • Polyethylene glycol 3,350 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    F1
    • Polyethylene glycol 3,350 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 2 M -
    		pH 4.6
    588 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    231 μl of water (100 v/v)
    F2
    • Polyethylene glycol 3,350 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 2 M -
    		pH 4.6
    588 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    71 μl of water (100 v/v)
    F3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 4.6
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    F4
    • Polyethylene glycol 3,350 4 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 1 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    294 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    525 μl of water (100 v/v)
    F5
    • Polyethylene glycol 3,350 12 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    40 μl of MES pH 6.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    601 μl of water (100 v/v)
    F6
    • Polyethylene glycol 3,350 20 w/v -
    • MES 0.1 M pH 5.8
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    5 μl of Calcium chloride (2 M)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    F7
    • Polyethylene glycol 3,350 4 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 1 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    294 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    525 μl of water (100 v/v)
    F8
    • Polyethylene glycol 3,350 12 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 0.2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    601 μl of water (100 v/v)
    F9
    • Polyethylene glycol 3,350 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    F10
    • Polyethylene glycol 3,350 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 1 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    294 μl of di-Sodium malonate (3.4 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    525 μl of water (100 v/v)
    F11
    • Polyethylene glycol 3,350 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    601 μl of water (100 v/v)
    F12
    • Polyethylene glycol 3,350 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 8.2
    5 μl of Calcium chloride (2 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    G1
    • Polyethylene glycol 6,000 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    		pH 4.6
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    420 μl of water (100 v/v)
    G2
    • Polyethylene glycol 6,000 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 0.2 M -
    		pH 4.6
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    620 μl of water (100 v/v)
    G3
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    G4
    • Polyethylene glycol 6,000 4 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    400 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    G5
    • Polyethylene glycol 6,000 12 w/v -
    • MES 0.1 M pH 5.8
    • Sodium chloride 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    620 μl of water (100 v/v)
    G6
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 5.8
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    G7
    • Polyethylene glycol 6,000 4 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    G8
    • Polyethylene glycol 6,000 12 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    620 μl of water (100 v/v)
    G9
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    G10
    • Polyethylene glycol 6,000 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    420 μl of water (100 v/v)
    G11
    • Polyethylene glycol 6,000 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Sodium chloride 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    620 μl of water (100 v/v)
    G12
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    H1
    • Polyethylene glycol 6,000 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 1 M -
    		pH 4.6
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    294 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    525 μl of water (100 v/v)
    H2
    • Polyethylene glycol 6,000 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 0.2 M -
    		pH 4.6
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    58 μl of di-Sodium malonate (3.4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    601 μl of water (100 v/v)
    H3
    • Polyethylene glycol 6,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 4.6
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    H4
    • Polyethylene glycol 6,000 4 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 0.5 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    147 μl of di-Sodium malonate (3.4 M)
    672 μl of water (100 v/v)
    H5
    • Polyethylene glycol 6,000 12 w/v -
    • MES 0.1 M pH 5.8
    • di-Sodium malonate 0.2 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    58 μl of di-Sodium malonate (3.4 M)
    601 μl of water (100 v/v)
    H6
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 5.8
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 5.8
    59 μl of MES pH 5 (1 M)
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of MES pH 6.5 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    H7
    • Polyethylene glycol 6,000 4 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 0.5 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    147 μl of di-Sodium malonate (3.4 M)
    672 μl of water (100 v/v)
    H8
    • Polyethylene glycol 6,000 12 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium malonate 0.2 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    58 μl of di-Sodium malonate (3.4 M)
    601 μl of water (100 v/v)
    H9
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 7
    63 μl of HEPES pH 6.5 (1 M)
    36 μl of HEPES pH 7.5 (1 M)
    5 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)
    H10
    • Polyethylene glycol 6,000 4 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Polyethylene glycol 6,000 (50 w/v)
    588 μl of di-Sodium malonate (3.4 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    231 μl of water (100 v/v)
    H11
    • Polyethylene glycol 6,000 12 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • di-Sodium malonate 0.2 M -
    		pH 8.2
    42 μl of tris chloride BCC pH 8.5 (1 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    58 μl of di-Sodium malonate (3.4 M)
    57 μl of tris chloride BCC pH 8 (1 M)
    601 μl of water (100 v/v)
    H12
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.2
    • Calcium chloride 0.01 M -
    • Magnesium chloride 0.01 M -
    		pH 8.2
    5 μl of Calcium chloride (2 M)
    42 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    57 μl of tris chloride BCC pH 8 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    491 μl of water (100 v/v)