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Supplemental Information

Screen Description

PGA

Created 2009-12-15.

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Barcodes

PGA

No barcodes found.

Screen Structure

PGA

Well Count 96
Number of distinct ingredients 21
Internal diversity (score mean dist) 0.763

Screen Composition

2025-08-16T09:05:43.112169 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PGA
Buffer 129
Organic (non-volatile) 18
Polymer 159
Salt 178

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PGA Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Potassium bromide 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
75.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 5 (1 M)
745.0 μl of water (100 v/v)
A2
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
100.0 μl of Magnesium chloride (2 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
720.0 μl of water (100 v/v)
A3
  • di-Sodium malonate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
88.2 μl of di-Sodium malonate pH 4 (3.4 M)
731.8 μl of water (100 v/v)
A4
  • Sodium formate 0.6 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
85.7 μl of Sodium formate (7 M)
734.3 μl of water (100 v/v)
A5
  • Ammonium formate 1 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
100.0 μl of Ammonium formate (10 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
720.0 μl of water (100 v/v)
A6
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
795.0 μl of water (100 v/v)
A7
  • L-Proline 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
200.0 μl of L-Proline (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
620.0 μl of water (100 v/v)
A8
  • L-Arginine 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 5
200.0 μl of L-Arginine (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
620.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
550.0 μl of water (100 v/v)
A10
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
550.0 μl of water (100 v/v)
A11
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
550.0 μl of water (100 v/v)
A12
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
450.0 μl of water (100 v/v)
B1
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 3,350 20 w/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
450.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 4,000 15 w/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
550.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 8,000 12 w/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
610.0 μl of water (100 v/v)
B4
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 20,000 8 w/v -
pH 5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
583.3 μl of water (100 v/v)
B5
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
495.0 μl of water (100 v/v)
B6
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
595.0 μl of water (100 v/v)
B7
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
595.0 μl of water (100 v/v)
B8
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
595.0 μl of water (100 v/v)
B9
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
695.0 μl of water (100 v/v)
B10
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
695.0 μl of water (100 v/v)
B11
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
695.0 μl of water (100 v/v)
B12
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5 (1 M)
695.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
498.6 μl of water (100 v/v)
C2
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
C3
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
C4
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
C5
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
C6
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
C7
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
C8
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium acetate 0.1 M pH 5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of Sodium acetate pH 5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
C9
  • Potassium bromide 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
75.0 μl of Potassium bromide (4 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
745.0 μl of water (100 v/v)
C10
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
720.0 μl of water (100 v/v)
C11
  • di-Sodium malonate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
88.2 μl of di-Sodium malonate pH 4 (3.4 M)
731.8 μl of water (100 v/v)
C12
  • Sodium formate 0.6 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
85.7 μl of Sodium formate (7 M)
734.3 μl of water (100 v/v)
D1
  • Ammonium formate 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
100.0 μl of Ammonium formate (10 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
720.0 μl of water (100 v/v)
D2
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
795.0 μl of water (100 v/v)
D3
  • L-Proline 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
200.0 μl of L-Proline (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
620.0 μl of water (100 v/v)
D4
  • L-Arginine 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 6.5
200.0 μl of L-Arginine (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
620.0 μl of water (100 v/v)
D5
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
D6
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
D7
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
D8
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
450.0 μl of water (100 v/v)
D9
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 3,350 20 w/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
450.0 μl of water (100 v/v)
D10
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
550.0 μl of water (100 v/v)
D11
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 8,000 12 w/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
610.0 μl of water (100 v/v)
D12
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 20,000 8 w/v -
pH 6.5
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
583.3 μl of water (100 v/v)
E1
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
495.0 μl of water (100 v/v)
E2
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
595.0 μl of water (100 v/v)
E3
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 6.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
595.0 μl of water (100 v/v)
E4
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
595.0 μl of water (100 v/v)
E5
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
695.0 μl of water (100 v/v)
E6
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
695.0 μl of water (100 v/v)
E7
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
695.0 μl of water (100 v/v)
E8
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 6.5
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
695.0 μl of water (100 v/v)
E9
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
498.6 μl of water (100 v/v)
E10
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
E11
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 6.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
E12
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
598.6 μl of water (100 v/v)
F1
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
F2
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
F4
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
42.9 μl of Sodium formate (7 M)
698.6 μl of water (100 v/v)
F5
  • Potassium bromide 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
75.0 μl of Potassium bromide (4 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
745.0 μl of water (100 v/v)
F6
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
100.0 μl of Magnesium chloride (2 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
720.0 μl of water (100 v/v)
F7
  • di-Sodium malonate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
88.2 μl of di-Sodium malonate pH 4 (3.4 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
731.8 μl of water (100 v/v)
F8
  • Sodium formate 0.6 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
85.7 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
734.3 μl of water (100 v/v)
F9
  • Ammonium formate 1 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
100.0 μl of Ammonium formate (10 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
720.0 μl of water (100 v/v)
F10
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
795.0 μl of water (100 v/v)
F11
  • L-Proline 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
200.0 μl of L-Proline (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
620.0 μl of water (100 v/v)
F12
  • L-Arginine 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 8 w/v -
pH 7.8
200.0 μl of L-Arginine (1 M)
80.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
620.0 μl of water (100 v/v)
G1
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G3
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 7.8
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G4
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
450.0 μl of water (100 v/v)
G5
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 3,350 20 w/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
450.0 μl of water (100 v/v)
G6
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 4,000 15 w/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G7
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 8,000 12 w/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
610.0 μl of water (100 v/v)
G8
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 5 w/v -
  • Polyethylene glycol 20,000 8 w/v -
pH 7.8
50.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
583.3 μl of water (100 v/v)
G9
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
495.0 μl of water (100 v/v)
G10
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
595.0 μl of water (100 v/v)
G11
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 7.8
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
595.0 μl of water (100 v/v)
G12
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
595.0 μl of water (100 v/v)
H1
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
695.0 μl of water (100 v/v)
H2
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
695.0 μl of water (100 v/v)
H3
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
695.0 μl of water (100 v/v)
H4
  • Potassium bromide 0.2 M -
  • Potassium thiocyanate 0.2 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 7.8
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
50.0 μl of Potassium bromide (4 M)
25.0 μl of Potassium thiocyanate (8 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
695.0 μl of water (100 v/v)
H5
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 400 30 v/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
498.6 μl of water (100 v/v)
H6
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
598.6 μl of water (100 v/v)
H7
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 7.8
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
598.6 μl of water (100 v/v)
H8
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
598.6 μl of water (100 v/v)
H9
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 3,350 5 w/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
698.6 μl of water (100 v/v)
H10
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 4,000 5 w/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
698.6 μl of water (100 v/v)
H11
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
698.6 μl of water (100 v/v)
H12
  • Ammonium sulfate 0.1 M -
  • Sodium formate 0.3 M -
  • tris chloride BCC 0.1 M pH 7.8
  • Poly-gamma-Glutamic acid 200-400 3 w/v -
  • Polyethylene glycol 20,000 3 w/v -
pH 7.8
28.6 μl of Ammonium sulfate (3.5 M)
30.0 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
100.0 μl of Polyethylene glycol 20,000 (30 w/v)
42.9 μl of Sodium formate (7 M)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
698.6 μl of water (100 v/v)