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Supplemental Information

Screen Description

PGA

Created 2009-12-15.

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PGA

    Screen Structure

    PGA

    Well Count 96
    Number of distinct ingredients 21
    Internal diversity (score mean dist) 0.763

    Screen Composition

    2025-06-14T13:46:52.900662 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PGA
    Buffer 129
    Organic (non-volatile) 18
    Polymer 159
    Salt 178

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PGA Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Potassium bromide 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    75 μl of Potassium bromide (4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    745 μl of water (100 v/v)
    A2
    • Magnesium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    100 μl of Magnesium chloride (2 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    A3
    • di-Sodium malonate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    88 μl of di-Sodium malonate pH 4 (3.4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    731 μl of water (100 v/v)
    A4
    • Sodium formate 0.6 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    85 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    734 μl of water (100 v/v)
    A5
    • Ammonium formate 1 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    100 μl of Ammonium formate (10 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    A6
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    795 μl of water (100 v/v)
    A7
    • L-Proline 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    200 μl of L-Proline (1 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    A8
    • L-Arginine 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 5
    200 μl of L-Arginine (1 M)
    100 μl of Sodium acetate pH 5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    A9
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    A10
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 5
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    A11
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    A12
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    B1
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 3,350 20 w/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    B2
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 4,000 15 w/v -
    pH 5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    B3
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 8,000 12 w/v -
    pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    610 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 20,000 8 w/v -
    pH 5
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    583 μl of water (100 v/v)
    B5
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    495 μl of water (100 v/v)
    B6
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 5
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    B7
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    B8
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    B9
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    B10
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    B11
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    695 μl of water (100 v/v)
    B12
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 5
    50 μl of Potassium bromide (4 M)
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of Sodium acetate pH 5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    C1
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 5
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    498 μl of water (100 v/v)
    C2
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 5
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    C3
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    C4
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 5
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    C5
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 5
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 5
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    C7
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 5
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    698 μl of water (100 v/v)
    C8
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium acetate 0.1 M pH 5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 5
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    42 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    C9
    • Potassium bromide 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    75 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    745 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    C11
    • di-Sodium malonate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    88 μl of di-Sodium malonate pH 4 (3.4 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    731 μl of water (100 v/v)
    C12
    • Sodium formate 0.6 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    85 μl of Sodium formate (7 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    734 μl of water (100 v/v)
    D1
    • Ammonium formate 1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Ammonium formate (10 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    D2
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    795 μl of water (100 v/v)
    D3
    • L-Proline 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of L-Proline (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    D4
    • L-Arginine 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 6.5
    200 μl of L-Arginine (1 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    D5
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    D6
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    D7
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    D8
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    D9
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 3,350 20 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    D10
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    D11
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 8,000 12 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    610 μl of water (100 v/v)
    D12
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 20,000 8 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    583 μl of water (100 v/v)
    E1
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    495 μl of water (100 v/v)
    E2
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 6.5
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    E3
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    E4
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    E5
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    E6
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    E7
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    695 μl of water (100 v/v)
    E8
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 6.5
    50 μl of Potassium bromide (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    E9
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    42 μl of Sodium formate (7 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    498 μl of water (100 v/v)
    E10
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 6.5
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    E11
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    E12
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    F1
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    42 μl of Sodium formate (7 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    F2
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    698 μl of water (100 v/v)
    F4
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    42 μl of Sodium formate (7 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    F5
    • Potassium bromide 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    75 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    745 μl of water (100 v/v)
    F6
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    F7
    • di-Sodium malonate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    88 μl of di-Sodium malonate pH 4 (3.4 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    731 μl of water (100 v/v)
    F8
    • Sodium formate 0.6 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    85 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    734 μl of water (100 v/v)
    F9
    • Ammonium formate 1 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Ammonium formate (10 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    720 μl of water (100 v/v)
    F10
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    795 μl of water (100 v/v)
    F11
    • L-Proline 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    200 μl of L-Proline (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    F12
    • L-Arginine 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 8 w/v -
    pH 7.8
    200 μl of L-Arginine (1 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    80 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    620 μl of water (100 v/v)
    G1
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    G2
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 7.8
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    G3
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    G4
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 3,350 20 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    450 μl of water (100 v/v)
    G6
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    550 μl of water (100 v/v)
    G7
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 8,000 12 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    610 μl of water (100 v/v)
    G8
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 5 w/v -
    • Polyethylene glycol 20,000 8 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    583 μl of water (100 v/v)
    G9
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    495 μl of water (100 v/v)
    G10
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 7.8
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    G11
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    G12
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    595 μl of water (100 v/v)
    H1
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    H2
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    H3
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    695 μl of water (100 v/v)
    H4
    • Potassium bromide 0.2 M -
    • Potassium thiocyanate 0.2 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 7.8
    50 μl of Potassium bromide (4 M)
    55 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    25 μl of Potassium thiocyanate (8 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    695 μl of water (100 v/v)
    H5
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 400 30 v/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    498 μl of water (100 v/v)
    H6
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 7.8
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    55 μl of tris chloride BCC pH 8 (1 M)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    H7
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    H8
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    598 μl of water (100 v/v)
    H9
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 3,350 5 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    H10
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)
    H11
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 8,000 5 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    698 μl of water (100 v/v)
    H12
    • Ammonium sulfate 0.1 M -
    • Sodium formate 0.3 M -
    • tris chloride BCC 0.1 M pH 7.8
    • Poly-gamma-Glutamic acid 200-400 3 w/v -
    • Polyethylene glycol 20,000 3 w/v -
    pH 7.8
    55 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Polyethylene glycol 20,000 (30 w/v)
    42 μl of Sodium formate (7 M)
    45 μl of tris chloride BCC pH 7.5 (1 M)
    30 μl of Poly-gamma-Glutamic acid 200-400 (100 w/v)
    28 μl of Ammonium sulfate (3.5 M)
    698 μl of water (100 v/v)