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Supplemental Information

Screen Description

Crystal Screen Cryo 2

Hampton Research / Crystal Screen Cryo 2
Created 2009-12-19.

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Crystal Screen Cryo 2

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Crystal Screen Cryo 2

    Screen Structure

    Crystal Screen Cryo 2

    Well Count 48
    Number of distinct ingredients 46
    Internal diversity (score mean dist) 0.845

    Screen Composition

    More information about screen composition chart.
    2025-06-14T14:03:39.294467 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Crystal Screen Cryo 2
    Buffer 46
    Cryoprotectant 41
    Detergent 1
    Organic (non-volatile) 57
    Organic (volatile) 6
    Polymer 18
    Salt 53

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Crystal Screen Cryo 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 1.6 M -
    • Polyethylene glycol 6,000 8 w/v -
    • Glycerol 20 v/v -
    		NA
    320 μl of Sodium chloride (5 M)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    320 μl of water (100 v/v)
    A2
    • Sodium chloride 0.3 M -
    • Magnesium chloride 0.006 M -
    • Cetyltrimethylammonium bromide 0.01 mM -
    • Glycerol 40 v/v -
    		NA
    60 μl of Sodium chloride (5 M)
    400 μl of Glycerol (100 v/v)
    0 μl of Cetyltrimethylammonium bromide (50 mM)
    3 μl of Magnesium chloride (2 M)
    536 μl of water (100 v/v)
    A3
    • Ethylene glycol 21.2 v/v -
    • Glycerol 15 v/v -
    		NA
    212 μl of Ethylene glycol (100 v/v)
    150 μl of Glycerol (100 v/v)
    637 μl of water (100 v/v)
    A4
    • 1,4-Dioxane 26.2 v/v -
    • Glycerol 25 v/v -
    		NA
    250 μl of Glycerol (100 v/v)
    262 μl of 1,4-Dioxane (100 v/v)
    487 μl of water (100 v/v)
    A5
    • Ammonium sulfate 1.5 M -
    • 2-Propanol 3.75 v/v -
    • Glycerol 25 v/v -
    		NA
    428 μl of Ammonium sulfate (3.5 M)
    250 μl of Glycerol (100 v/v)
    37 μl of 2-Propanol (100 v/v)
    283 μl of water (100 v/v)
    A6
    • Imidazole 0.65 M -
    • Glycerol 35 v/v -
    		NA
    350 μl of Glycerol (100 v/v)
    650 μl of Imidazole pH 7 (1 M)
    A7
    • Polyethylene glycol 1,000 8 w/v -
    • Polyethylene glycol 8,000 8 w/v -
    • Glycerol 20 v/v -
    		NA
    160 μl of Polyethylene glycol 1,000 (50 w/v)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    480 μl of water (100 v/v)
    A8
    • Sodium chloride 1.05 M -
    • Ethanol 7 v/v -
    • Glycerol 30 v/v -
    		NA
    70 μl of Ethanol (100 v/v)
    210 μl of Sodium chloride (5 M)
    300 μl of Glycerol (100 v/v)
    420 μl of water (100 v/v)
    B1
    • Sodium acetate 0.08 M pH 4.6
    • Sodium chloride 1.5 M -
    • Glycerol 25 v/v -
    		pH 4.6
    80 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Sodium chloride (5 M)
    250 μl of Glycerol (100 v/v)
    370 μl of water (100 v/v)
    B2
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Sodium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    560 μl of water (100 v/v)
    B3
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.08 M pH 4.6
    • 1,6-Hexanediol 0.8 M -
    • Glycerol 20 v/v -
    		pH 4.6
    10 μl of Cobalt (II) chloride (1 M)
    133 μl of 1,6-Hexanediol (6 M)
    80 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of Glycerol (100 v/v)
    576 μl of water (100 v/v)
    B4
    • Cadmium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 28.5 v/v -
    • Glycerol 5 v/v -
    		pH 4.6
    285 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Cadmium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    50 μl of Glycerol (100 v/v)
    465 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.18 M -
    • Sodium acetate 0.09 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 27 w/v -
    • Glycerol 10 v/v -
    		pH 4.6
    51 μl of Ammonium sulfate (3.5 M)
    540 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    90 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Glycerol (100 v/v)
    218 μl of water (100 v/v)
    B6
    • Potassium Sodium tartrate 0.15 M -
    • tri-Sodium citrate 0.08 M pH 5.6
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    		pH 5.6
    100 μl of Potassium Sodium tartrate (1.5 M)
    428 μl of Ammonium sulfate (3.5 M)
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    250 μl of Glycerol (100 v/v)
    141 μl of water (100 v/v)
    B7
    • Ammonium sulfate 0.38 M -
    • tri-Sodium citrate 0.08 M pH 5.6
    • Lithium sulfate 0.75 M -
    • Glycerol 25 v/v -
    		pH 5.6
    108 μl of Ammonium sulfate (3.5 M)
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    250 μl of Glycerol (100 v/v)
    375 μl of Lithium sulfate (2 M)
    186 μl of water (100 v/v)
    B8
    • Sodium chloride 0.3 M -
    • tri-Sodium citrate 0.06 M pH 5.6
    • Ethylene imine polymer 1.2 v/v -
    • Glycerol 40 v/v -
    		pH 5.6
    60 μl of tri-Sodium citrate pH 5.6 (1 M)
    60 μl of Sodium chloride (5 M)
    400 μl of Glycerol (100 v/v)
    24 μl of Ethylene imine polymer (50 v/v)
    456 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.08 M pH 5.6
    • 2-Methyl-2-propanol 28 v/v -
    • Glycerol 20 v/v -
    		pH 5.6
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Glycerol (100 v/v)
    700 μl of 2-Methyl-2-propanol (40 v/v)
    20 μl of water (100 v/v)
    C2
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.07 M pH 5.6
    • Jeffamine® M-600 7 v/v -
    • Glycerol 30 v/v -
    		pH 5.6
    70 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of Glycerol (100 v/v)
    10 μl of Iron (III) chloride (1 M)
    140 μl of Jeffamine® M-600 pH 7 (50 v/v)
    480 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.38 M -
    • Glycerol 5 v/v -
    		pH 5.6
    396 μl of 1,6-Hexanediol (6 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Glycerol (100 v/v)
    453 μl of water (100 v/v)
    C4
    • MES 0.08 M pH 6.5
    • Magnesium sulfate 1.28 M -
    • Glycerol 20 v/v -
    		pH 6.5
    640 μl of Magnesium sulfate (2 M)
    80 μl of MES pH 6.5 (1 M)
    200 μl of Glycerol (100 v/v)
    80 μl of water (100 v/v)
    C5
    • Sodium di-hydrogen phosphate 0.08 M -
    • Potassium di-hydrogen phosphate 0.075 M -
    • MES 0.08 M pH 6.5
    • Sodium chloride 1.5 M -
    • Glycerol 25 v/v -
    		pH 6.5
    80 μl of MES pH 6.5 (1 M)
    20 μl of Sodium di-hydrogen phosphate (4 M)
    300 μl of Sodium chloride (5 M)
    250 μl of Glycerol (100 v/v)
    50 μl of Potassium di-hydrogen phosphate (1.5 M)
    300 μl of water (100 v/v)
    C6
    • MES 0.07 M pH 6.5
    • Polyethylene glycol 20,000 7.8 w/v -
    • Glycerol 35 v/v -
    		pH 6.5
    70 μl of MES pH 6.5 (1 M)
    350 μl of Glycerol (100 v/v)
    260 μl of Polyethylene glycol 20,000 (30 w/v)
    320 μl of water (100 v/v)
    C7
    • Ammonium sulfate 1.2 M -
    • MES 0.08 M pH 6.5
    • 1,4-Dioxane 7.5 v/v -
    • Glycerol 25 v/v -
    		pH 6.5
    342 μl of Ammonium sulfate (3.5 M)
    80 μl of MES pH 6.5 (1 M)
    250 μl of Glycerol (100 v/v)
    75 μl of 1,4-Dioxane (100 v/v)
    252 μl of water (100 v/v)
    C8
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    250 μl of water (100 v/v)
    D1
    • Cobalt (II) chloride 0.01 M -
    • MES 0.08 M pH 6.5
    • Ammonium sulfate 1.35 M -
    • Glycerol 25 v/v -
    		pH 6.5
    10 μl of Cobalt (II) chloride (1 M)
    385 μl of Ammonium sulfate (3.5 M)
    80 μl of MES pH 6.5 (1 M)
    250 μl of Glycerol (100 v/v)
    274 μl of water (100 v/v)
    D2
    • Ammonium sulfate 0.18 M -
    • MES 0.09 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 27 w/v -
    • Glycerol 10 v/v -
    		pH 6.5
    51 μl of Ammonium sulfate (3.5 M)
    90 μl of MES pH 6.5 (1 M)
    100 μl of Glycerol (100 v/v)
    540 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    218 μl of water (100 v/v)
    D3
    • Zinc sulfate 0.01 M -
    • MES 0.09 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 22.5 v/v -
    • Glycerol 10 v/v -
    		pH 6.5
    90 μl of MES pH 6.5 (1 M)
    100 μl of Glycerol (100 v/v)
    5 μl of Zinc sulfate (2 M)
    225 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    580 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 1.6 M -
    		NA
    1000 μl of tri-Sodium citrate (1.6 M)
    D5
    • Ammonium sulfate 0.5 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 7.5
    142 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    457 μl of water (100 v/v)
    D6
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 6,000 8 w/v -
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • Glycerol 20 v/v -
    		pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    520 μl of water (100 v/v)
    D7
    • HEPES 0.09 M pH 7.5
    • Jeffamine® M-600 17 v/v -
    • Glycerol 15 v/v -
    		pH 7.5
    90 μl of HEPES pH 7.5 (1 M)
    150 μl of Glycerol (100 v/v)
    340 μl of Jeffamine® M-600 pH 7 (50 v/v)
    420 μl of water (100 v/v)
    D8
    • Sodium chloride 0.08 M -
    • HEPES 0.08 M pH 7.5
    • Ammonium sulfate 1.2 M -
    • Glycerol 25 v/v -
    		pH 7.5
    342 μl of Ammonium sulfate (3.5 M)
    80 μl of HEPES pH 7.5 (1 M)
    16 μl of Sodium chloride (5 M)
    250 μl of Glycerol (100 v/v)
    311 μl of water (100 v/v)
    E1
    • HEPES 0.07 M pH 7.5
    • Ammonium formate 1.4 M -
    • Glycerol 30 v/v -
    		pH 7.5
    70 μl of HEPES pH 7.5 (1 M)
    140 μl of Ammonium formate (10 M)
    300 μl of Glycerol (100 v/v)
    490 μl of water (100 v/v)
    E2
    • Cadmium sulfate 0.04 M -
    • HEPES 0.08 M pH 7.5
    • Sodium acetate 0.75 M -
    • Glycerol 25 v/v -
    		pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    40 μl of Cadmium sulfate (1 M)
    250 μl of Sodium acetate (3 M)
    380 μl of water (100 v/v)
    E3
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of water (100 v/v)
    E4
    • HEPES 0.09 M pH 7.5
    • Sodium chloride 3.66 M -
    • Glycerol 15 v/v -
    		pH 7.5
    90 μl of HEPES pH 7.5 (1 M)
    732 μl of Sodium chloride (5 M)
    150 μl of Glycerol (100 v/v)
    28 μl of water (100 v/v)
    E5
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 8,000 7.5 w/v -
    • Ethylene glycol 6 v/v -
    • Glycerol 25 v/v -
    		pH 7.5
    60 μl of Ethylene glycol (100 v/v)
    80 μl of HEPES pH 7.5 (1 M)
    150 μl of Polyethylene glycol 8,000 (50 w/v)
    250 μl of Glycerol (100 v/v)
    460 μl of water (100 v/v)
    E6
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 10,000 15 w/v -
    • Glycerol 25 v/v -
    		pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    370 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 M -
    		pH 8.5
    566 μl of 1,6-Hexanediol (6 M)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    E8
    • tris chloride BCC 0.08 M pH 8.5
    • 2-Methyl-2-propanol 18.8 v/v -
    • Glycerol 25 v/v -
    		pH 8.5
    250 μl of Glycerol (100 v/v)
    468 μl of 2-Methyl-2-propanol (40 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    201 μl of water (100 v/v)
    F1
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Lithium sulfate 0.75 M -
    • Glycerol 25 v/v -
    		pH 8.5
    250 μl of Glycerol (100 v/v)
    375 μl of Lithium sulfate (2 M)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    2 μl of Nickel (II) chloride (4 M)
    292 μl of water (100 v/v)
    F2
    • Ammonium sulfate 1.28 M -
    • tris chloride BCC 0.09 M pH 8.5
    • Glycerol 25.2 v/v -
    		pH 8.5
    365 μl of Ammonium sulfate (3.5 M)
    252 μl of Glycerol (100 v/v)
    90 μl of tris chloride BCC pH 8.5 (1 M)
    292 μl of water (100 v/v)
    F3
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    		pH 8.5
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    320 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.08 M pH 8.5
    • Ethanol 15 v/v -
    • Glycerol 25 v/v -
    		pH 8.5
    150 μl of Ethanol (100 v/v)
    250 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    520 μl of water (100 v/v)
    F5
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    • Glycerol 20 v/v -
    		pH 8.5
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    2 μl of Nickel (II) chloride (4 M)
    397 μl of water (100 v/v)
    F6
    • Sodium chloride 0.09 M -
    • BICINE 0.09 M pH 9
    • Polyethylene glycol monomethyl ether 550 17 v/v -
    • Glycerol 15 v/v -
    		pH 9
    90 μl of BICINE pH 9 (1 M)
    18 μl of Sodium chloride (5 M)
    150 μl of Glycerol (100 v/v)
    170 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    572 μl of water (100 v/v)
    F7
    • BICINE 0.1 M pH 9
    • Magnesium chloride 1.9 M -
    • Glycerol 5 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    380 μl of Magnesium chloride (5 M)
    50 μl of Glycerol (100 v/v)
    470 μl of water (100 v/v)
    F8
    • BICINE 0.07 M pH 9
    • 1,4-Dioxane 1.4 v/v -
    • Polyethylene glycol 20,000 7 w/v -
    • Glycerol 30 v/v -
    		pH 9
    70 μl of BICINE pH 9 (1 M)
    300 μl of Glycerol (100 v/v)
    233 μl of Polyethylene glycol 20,000 (30 w/v)
    14 μl of 1,4-Dioxane (100 v/v)
    382 μl of water (100 v/v)