C6 - Screen Contents - Crystal Screen Cryo 2

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Supplemental Information

Screen Description

Crystal Screen Cryo 2

Hampton Research / Crystal Screen Cryo 2

Created 2009-12-19.

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Crystal Screen Cryo 2

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Crystal Screen Cryo 2

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Screen Structure

Crystal Screen Cryo 2

Well Count	48
Number of distinct ingredients	46
Internal diversity (score mean dist)	0.845

Screen Composition

More information about screen composition chart.

ingredient type	Crystal Screen Cryo 2
Buffer	46
Cryoprotectant	41
Detergent	1
Organic (non-volatile)	57
Organic (volatile)	6
Polymer	18
Salt	53

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Crystal Screen Cryo 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 1.6 M - Polyethylene glycol 6,000 8 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 160.0 μl of Polyethylene glycol 6,000 (50 w/v) 320.0 μl of Sodium chloride (5 M) 320.0 μl of water (100 v/v)
A2	Sodium chloride 0.3 M - Magnesium chloride 0.006 M - Cetyltrimethylammonium bromide 0.01 mM - Glycerol 40 v/v - 🔍	NA	0.2 μl of Cetyltrimethylammonium bromide (50 mM) 400.0 μl of Glycerol (100 v/v) 3.0 μl of Magnesium chloride (2 M) 60.0 μl of Sodium chloride (5 M) 536.8 μl of water (100 v/v)
A3	Ethylene glycol 21.2 v/v - Glycerol 15 v/v - 🔍	NA	212.5 μl of Ethylene glycol (100 v/v) 150.0 μl of Glycerol (100 v/v) 637.5 μl of water (100 v/v)
A4	1,4-Dioxane 26.2 v/v - Glycerol 25 v/v - 🔍	NA	262.5 μl of 1,4-Dioxane (100 v/v) 250.0 μl of Glycerol (100 v/v) 487.5 μl of water (100 v/v)
A5	Ammonium sulfate 1.5 M - 2-Propanol 3.75 v/v - Glycerol 25 v/v - 🔍	NA	37.5 μl of 2-Propanol (100 v/v) 428.6 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 283.9 μl of water (100 v/v)
A6	Imidazole 0.65 M - Glycerol 35 v/v - 🔍	NA	350.0 μl of Glycerol (100 v/v) 650.0 μl of Imidazole pH 7 (1 M)
A7	Polyethylene glycol 1,000 8 w/v - Polyethylene glycol 8,000 8 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 160.0 μl of Polyethylene glycol 1,000 (50 w/v) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 480.0 μl of water (100 v/v)
A8	Sodium chloride 1.05 M - Ethanol 7 v/v - Glycerol 30 v/v - 🔍	NA	70.0 μl of Ethanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 210.0 μl of Sodium chloride (5 M) 420.0 μl of water (100 v/v)
B1	Sodium acetate 0.08 M pH 4.6 Sodium chloride 1.5 M - Glycerol 25 v/v - 🔍	pH 4.6	250.0 μl of Glycerol (100 v/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 300.0 μl of Sodium chloride (5 M) 370.0 μl of water (100 v/v)
B2	Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 40.0 μl of Sodium chloride (5 M) 560.0 μl of water (100 v/v)
B3	Cobalt (II) chloride 0.01 M - Sodium acetate 0.08 M pH 4.6 1,6-Hexanediol 0.8 M - Glycerol 20 v/v - 🔍	pH 4.6	133.3 μl of 1,6-Hexanediol (6 M) 10.0 μl of Cobalt (II) chloride (1 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 576.7 μl of water (100 v/v)
B4	Cadmium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 28.5 v/v - Glycerol 5 v/v - 🔍	pH 4.6	100.0 μl of Cadmium chloride (1 M) 50.0 μl of Glycerol (100 v/v) 285.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 465.0 μl of water (100 v/v)
B5	Ammonium sulfate 0.18 M - Sodium acetate 0.09 M pH 4.6 Polyethylene glycol monomethyl ether 2,000 27 w/v - Glycerol 10 v/v - 🔍	pH 4.6	51.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Glycerol (100 v/v) 540.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 90.0 μl of Sodium acetate pH 4.6 (1 M) 218.6 μl of water (100 v/v)
B6	Potassium Sodium tartrate 0.15 M - tri-Sodium citrate 0.08 M pH 5.6 Ammonium sulfate 1.5 M - Glycerol 25 v/v - 🔍	pH 5.6	428.6 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 100.0 μl of Potassium Sodium tartrate (1.5 M) 80.0 μl of tri-Sodium citrate pH 5.6 (1 M) 141.4 μl of water (100 v/v)
B7	Ammonium sulfate 0.38 M - tri-Sodium citrate 0.08 M pH 5.6 Lithium sulfate 0.75 M - Glycerol 25 v/v - 🔍	pH 5.6	108.6 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 375.0 μl of Lithium sulfate (2 M) 80.0 μl of tri-Sodium citrate pH 5.6 (1 M) 186.4 μl of water (100 v/v)
B8	Sodium chloride 0.3 M - tri-Sodium citrate 0.06 M pH 5.6 Ethylene imine polymer 1.2 v/v - Glycerol 40 v/v - 🔍	pH 5.6	24.0 μl of Ethylene imine polymer (50 v/v) 400.0 μl of Glycerol (100 v/v) 60.0 μl of Sodium chloride (5 M) 60.0 μl of tri-Sodium citrate pH 5.6 (1 M) 456.0 μl of water (100 v/v)
C1	tri-Sodium citrate 0.08 M pH 5.6 2-Methyl-2-propanol 28 v/v - Glycerol 20 v/v - 🔍	pH 5.6	700.0 μl of 2-Methyl-2-propanol (40 v/v) 200.0 μl of Glycerol (100 v/v) 80.0 μl of tri-Sodium citrate pH 5.6 (1 M) 20.0 μl of water (100 v/v)
C2	Iron (III) chloride 0.01 M - tri-Sodium citrate 0.07 M pH 5.6 Jeffamine® M-600 7 v/v - Glycerol 30 v/v - 🔍	pH 5.6	300.0 μl of Glycerol (100 v/v) 10.0 μl of Iron (III) chloride (1 M) 140.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 70.0 μl of tri-Sodium citrate pH 5.6 (1 M) 480.0 μl of water (100 v/v)
C3	tri-Sodium citrate 0.1 M pH 5.6 1,6-Hexanediol 2.38 M - Glycerol 5 v/v - 🔍	pH 5.6	396.7 μl of 1,6-Hexanediol (6 M) 50.0 μl of Glycerol (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 453.3 μl of water (100 v/v)
C4	MES 0.08 M pH 6.5 Magnesium sulfate 1.28 M - Glycerol 20 v/v - 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 640.0 μl of Magnesium sulfate (2 M) 80.0 μl of water (100 v/v)
C5	Sodium di-hydrogen phosphate 0.08 M - Potassium di-hydrogen phosphate 0.075 M - MES 0.08 M pH 6.5 Sodium chloride 1.5 M - Glycerol 25 v/v - 🔍	pH 6.5	250.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 50.0 μl of Potassium di-hydrogen phosphate (1.5 M) 300.0 μl of Sodium chloride (5 M) 20.0 μl of Sodium di-hydrogen phosphate (4 M) 300.0 μl of water (100 v/v)
C6	MES 0.07 M pH 6.5 Polyethylene glycol 20,000 7.8 w/v - Glycerol 35 v/v - 🔍	pH 6.5	350.0 μl of Glycerol (100 v/v) 70.0 μl of MES pH 6.5 (1 M) 260.0 μl of Polyethylene glycol 20,000 (30 w/v) 320.0 μl of water (100 v/v)
C7	Ammonium sulfate 1.2 M - MES 0.08 M pH 6.5 1,4-Dioxane 7.5 v/v - Glycerol 25 v/v - 🔍	pH 6.5	75.0 μl of 1,4-Dioxane (100 v/v) 342.9 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 252.1 μl of water (100 v/v)
C8	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
D1	Cobalt (II) chloride 0.01 M - MES 0.08 M pH 6.5 Ammonium sulfate 1.35 M - Glycerol 25 v/v - 🔍	pH 6.5	385.7 μl of Ammonium sulfate (3.5 M) 10.0 μl of Cobalt (II) chloride (1 M) 250.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 274.3 μl of water (100 v/v)
D2	Ammonium sulfate 0.18 M - MES 0.09 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 27 w/v - Glycerol 10 v/v - 🔍	pH 6.5	51.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Glycerol (100 v/v) 90.0 μl of MES pH 6.5 (1 M) 540.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 218.6 μl of water (100 v/v)
D3	Zinc sulfate 0.01 M - MES 0.09 M pH 6.5 Polyethylene glycol monomethyl ether 550 22.5 v/v - Glycerol 10 v/v - 🔍	pH 6.5	100.0 μl of Glycerol (100 v/v) 90.0 μl of MES pH 6.5 (1 M) 225.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 5.0 μl of Zinc sulfate (2 M) 580.0 μl of water (100 v/v)
D4	tri-Sodium citrate 1.6 M - 🔍	NA	1000.0 μl of tri-Sodium citrate (1.6 M)
D5	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 142.9 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 457.1 μl of water (100 v/v)
D6	HEPES 0.08 M pH 7.5 Polyethylene glycol 6,000 8 w/v - 2-methyl-2,4-pentanediol BCC 4 v/v - Glycerol 20 v/v - 🔍	pH 7.5	40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Glycerol (100 v/v) 80.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol 6,000 (50 w/v) 520.0 μl of water (100 v/v)
D7	HEPES 0.09 M pH 7.5 Jeffamine® M-600 17 v/v - Glycerol 15 v/v - 🔍	pH 7.5	150.0 μl of Glycerol (100 v/v) 90.0 μl of HEPES pH 7.5 (1 M) 340.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 420.0 μl of water (100 v/v)
D8	Sodium chloride 0.08 M - HEPES 0.08 M pH 7.5 Ammonium sulfate 1.2 M - Glycerol 25 v/v - 🔍	pH 7.5	342.9 μl of Ammonium sulfate (3.5 M) 250.0 μl of Glycerol (100 v/v) 80.0 μl of HEPES pH 7.5 (1 M) 16.0 μl of Sodium chloride (5 M) 311.1 μl of water (100 v/v)
E1	HEPES 0.07 M pH 7.5 Ammonium formate 1.4 M - Glycerol 30 v/v - 🔍	pH 7.5	140.0 μl of Ammonium formate (10 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of HEPES pH 7.5 (1 M) 490.0 μl of water (100 v/v)
E2	Cadmium sulfate 0.04 M - HEPES 0.08 M pH 7.5 Sodium acetate 0.75 M - Glycerol 25 v/v - 🔍	pH 7.5	40.0 μl of Cadmium sulfate (1 M) 250.0 μl of Glycerol (100 v/v) 80.0 μl of HEPES pH 7.5 (1 M) 250.0 μl of Sodium acetate (3 M) 380.0 μl of water (100 v/v)
E3	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 70 v/v - 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v)
E4	HEPES 0.09 M pH 7.5 Sodium chloride 3.66 M - Glycerol 15 v/v - 🔍	pH 7.5	150.0 μl of Glycerol (100 v/v) 90.0 μl of HEPES pH 7.5 (1 M) 732.0 μl of Sodium chloride (5 M) 28.0 μl of water (100 v/v)
E5	HEPES 0.08 M pH 7.5 Polyethylene glycol 8,000 7.5 w/v - Ethylene glycol 6 v/v - Glycerol 25 v/v - 🔍	pH 7.5	60.0 μl of Ethylene glycol (100 v/v) 250.0 μl of Glycerol (100 v/v) 80.0 μl of HEPES pH 7.5 (1 M) 150.0 μl of Polyethylene glycol 8,000 (50 w/v) 460.0 μl of water (100 v/v)
E6	HEPES 0.08 M pH 7.5 Polyethylene glycol 10,000 15 w/v - Glycerol 25 v/v - 🔍	pH 7.5	250.0 μl of Glycerol (100 v/v) 80.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 370.0 μl of water (100 v/v)
E7	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 1,6-Hexanediol 3.4 M - 🔍	pH 8.5	566.7 μl of 1,6-Hexanediol (6 M) 100.0 μl of Magnesium chloride (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v)
E8	tris chloride BCC 0.08 M pH 8.5 2-Methyl-2-propanol 18.8 v/v - Glycerol 25 v/v - 🔍	pH 8.5	468.8 μl of 2-Methyl-2-propanol (40 v/v) 250.0 μl of Glycerol (100 v/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 201.2 μl of water (100 v/v)
F1	Nickel (II) chloride 0.01 M - tris chloride BCC 0.08 M pH 8.5 Lithium sulfate 0.75 M - Glycerol 25 v/v - 🔍	pH 8.5	250.0 μl of Glycerol (100 v/v) 375.0 μl of Lithium sulfate (2 M) 2.5 μl of Nickel (II) chloride (4 M) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 292.5 μl of water (100 v/v)
F2	Ammonium sulfate 1.28 M - tris chloride BCC 0.09 M pH 8.5 Glycerol 25.2 v/v - 🔍	pH 8.5	365.7 μl of Ammonium sulfate (3.5 M) 252.0 μl of Glycerol (100 v/v) 90.0 μl of tris chloride BCC pH 8.5 (1 M) 292.3 μl of water (100 v/v)
F3	Ammonium di-hydrogen phosphate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
F4	tris chloride BCC 0.08 M pH 8.5 Ethanol 15 v/v - Glycerol 25 v/v - 🔍	pH 8.5	150.0 μl of Ethanol (100 v/v) 250.0 μl of Glycerol (100 v/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v)
F5	Nickel (II) chloride 0.01 M - tris chloride BCC 0.08 M pH 8.5 Polyethylene glycol monomethyl ether 2,000 16 w/v - Glycerol 20 v/v - 🔍	pH 8.5	200.0 μl of Glycerol (100 v/v) 2.5 μl of Nickel (II) chloride (4 M) 320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
F6	Sodium chloride 0.09 M - BICINE 0.09 M pH 9 Polyethylene glycol monomethyl ether 550 17 v/v - Glycerol 15 v/v - 🔍	pH 9	90.0 μl of BICINE pH 9 (1 M) 150.0 μl of Glycerol (100 v/v) 170.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 18.0 μl of Sodium chloride (5 M) 572.0 μl of water (100 v/v)
F7	BICINE 0.1 M pH 9 Magnesium chloride 1.9 M - Glycerol 5 v/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 50.0 μl of Glycerol (100 v/v) 380.0 μl of Magnesium chloride (5 M) 470.0 μl of water (100 v/v)
F8	BICINE 0.07 M pH 9 1,4-Dioxane 1.4 v/v - Polyethylene glycol 20,000 7 w/v - Glycerol 30 v/v - 🔍	pH 9	14.0 μl of 1,4-Dioxane (100 v/v) 70.0 μl of BICINE pH 9 (1 M) 300.0 μl of Glycerol (100 v/v) 233.3 μl of Polyethylene glycol 20,000 (30 w/v) 382.7 μl of water (100 v/v)