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Supplemental Information

Screen Description

Crystal screen Cryo HT

Hampton Research / Crystal screen Cryo HT
Created 2009-12-19.

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Crystal screen Cryo HT

    Screen Structure

    Crystal screen Cryo HT

    Well Count 96
    Number of distinct ingredients 56
    Internal diversity (score mean dist) 0.843

    Screen Composition

    2025-06-13T21:36:28.918205 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Crystal screen Cryo HT
    Buffer 108
    Cryoprotectant 83
    Detergent 1
    Organic (non-volatile) 107
    Organic (volatile) 13
    Polymer 41
    Salt 116

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Crystal screen Cryo HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    10 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    590 μl of water (100 v/v)
    A2
    • Potassium Sodium tartrate 0.26 M -
    • Glycerol 35 v/v -
    NA
    173 μl of Potassium Sodium tartrate (1.5 M)
    350 μl of Glycerol (100 v/v)
    476 μl of water (100 v/v)
    A3
    • Ammonium di-hydrogen phosphate 0.26 M -
    • Glycerol 35 v/v -
    NA
    350 μl of Glycerol (100 v/v)
    104 μl of Ammonium di-hydrogen phosphate (2.5 M)
    546 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.075 M pH 8.5
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    pH 8.5
    250 μl of Glycerol (100 v/v)
    75 μl of tris chloride BCC pH 8.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    246 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    475 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.16 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Polyethylene glycol 4,000 24 w/v -
    • Glycerol 20 v/v -
    pH 8.5
    80 μl of Magnesium chloride (2 M)
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of water (100 v/v)
    A7
    • Sodium cacodylate 0.07 M pH 6.5
    • Sodium acetate 0.98 M -
    • Glycerol 30 v/v -
    pH 6.5
    326 μl of Sodium acetate (3 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium cacodylate pH 6.5 (1 M)
    303 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.14 M -
    • Sodium cacodylate 0.07 M pH 6.5
    • 2-Propanol 21 v/v -
    • Glycerol 30 v/v -
    pH 6.5
    300 μl of Glycerol (100 v/v)
    210 μl of 2-Propanol (100 v/v)
    70 μl of Sodium cacodylate pH 6.5 (1 M)
    87 μl of tri-Sodium citrate (1.6 M)
    332 μl of water (100 v/v)
    A9
    • Ammonium acetate 0.17 M -
    • tri-Sodium citrate 0.085 M pH 5.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 5.6
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    85 μl of tri-Sodium citrate pH 5.6 (1 M)
    170 μl of Ammonium acetate (1 M)
    85 μl of water (100 v/v)
    A10
    • Ammonium acetate 0.17 M -
    • Sodium acetate 0.085 M pH 4.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 4.6
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    85 μl of Sodium acetate pH 4.6 (1 M)
    170 μl of Ammonium acetate (1 M)
    85 μl of water (100 v/v)
    A11
    • tri-Sodium citrate 0.07 M pH 5.6
    • Ammonium di-hydrogen phosphate 0.7 M -
    • Glycerol 30 v/v -
    pH 5.6
    300 μl of Glycerol (100 v/v)
    280 μl of Ammonium di-hydrogen phosphate (2.5 M)
    70 μl of tri-Sodium citrate pH 5.6 (1 M)
    350 μl of water (100 v/v)
    A12
    • Magnesium chloride 0.18 M -
    • HEPES 0.09 M pH 7.5
    • 2-Propanol 27 v/v -
    • Glycerol 10 v/v -
    pH 7.5
    90 μl of Magnesium chloride (2 M)
    90 μl of HEPES pH 7.5 (1 M)
    100 μl of Glycerol (100 v/v)
    270 μl of 2-Propanol (100 v/v)
    450 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    125 μl of tri-Sodium citrate (1.6 M)
    475 μl of water (100 v/v)
    B2
    • Calcium chloride 0.19 M -
    • HEPES 0.095 M pH 7.5
    • Polyethylene glycol 400 26.6 v/v -
    • Glycerol 5 v/v -
    pH 7.5
    95 μl of Calcium chloride (2 M)
    95 μl of HEPES pH 7.5 (1 M)
    50 μl of Glycerol (100 v/v)
    266 μl of Polyethylene glycol 400 (100 v/v)
    494 μl of water (100 v/v)
    B3
    • Ammonium sulfate 0.17 M -
    • Sodium cacodylate 0.085 M pH 6.5
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 6.5
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    85 μl of Sodium cacodylate pH 6.5 (1 M)
    48 μl of Ammonium sulfate (3.5 M)
    206 μl of water (100 v/v)
    B4
    • HEPES 0.075 M pH 7.5
    • Lithium sulfate 1.12 M -
    • Glycerol 25 v/v -
    pH 7.5
    75 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    562 μl of Lithium sulfate (2 M)
    112 μl of water (100 v/v)
    B5
    • Lithium sulfate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 8.5
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    85 μl of tris chloride BCC pH 8.5 (1 M)
    85 μl of Lithium sulfate (2 M)
    170 μl of water (100 v/v)
    B6
    • Magnesium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    pH 6.5
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Magnesium acetate (1 M)
    240 μl of water (100 v/v)
    B7
    • Ammonium acetate 0.16 M -
    • tris chloride BCC 0.08 M pH 8.5
    • 2-Propanol 24 v/v -
    • Glycerol 20 v/v -
    pH 8.5
    200 μl of Glycerol (100 v/v)
    240 μl of 2-Propanol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Ammonium acetate (1 M)
    320 μl of water (100 v/v)
    B8
    • Ammonium sulfate 0.16 M -
    • Sodium acetate 0.08 M pH 4.6
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 20 v/v -
    pH 4.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    45 μl of Ammonium sulfate (3.5 M)
    274 μl of water (100 v/v)
    B9
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Magnesium acetate (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    B10
    • Sodium acetate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 8.5
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    56 μl of Sodium acetate (3 M)
    150 μl of Glycerol (100 v/v)
    85 μl of tris chloride BCC pH 8.5 (1 M)
    198 μl of water (100 v/v)
    B11
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    100 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    B12
    • Calcium chloride 0.14 M -
    • Sodium acetate 0.07 M pH 4.6
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    pH 4.6
    70 μl of Calcium chloride (2 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    140 μl of 2-Propanol (100 v/v)
    420 μl of water (100 v/v)
    C1
    • Imidazole 0.07 M pH 6.5
    • Sodium acetate 0.7 M -
    • Glycerol 30 v/v -
    pH 6.5
    233 μl of Sodium acetate (3 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Imidazole pH 6.5 (1 M)
    396 μl of water (100 v/v)
    C2
    • Ammonium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium acetate (1 M)
    400 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.14 M -
    • HEPES 0.07 M pH 7.5
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    pH 7.5
    70 μl of HEPES pH 7.5 (1 M)
    300 μl of Glycerol (100 v/v)
    140 μl of 2-Propanol (100 v/v)
    87 μl of tri-Sodium citrate (1.6 M)
    402 μl of water (100 v/v)
    C4
    • Sodium acetate 0.17 M -
    • Sodium cacodylate 0.085 M pH 6.5
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 6.5
    56 μl of Sodium acetate (3 M)
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    85 μl of Sodium cacodylate pH 6.5 (1 M)
    198 μl of water (100 v/v)
    C5
    • HEPES 0.065 M pH 7.5
    • Potassium Sodium tartrate 0.52 M -
    • Glycerol 35 v/v -
    pH 7.5
    346 μl of Potassium Sodium tartrate (1.5 M)
    65 μl of HEPES pH 7.5 (1 M)
    350 μl of Glycerol (100 v/v)
    238 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 8,000 25.5 w/v -
    • Glycerol 15 v/v -
    NA
    510 μl of Polyethylene glycol 8,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    48 μl of Ammonium sulfate (3.5 M)
    291 μl of water (100 v/v)
    C7
    • Ammonium sulfate 0.17 M -
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    NA
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    48 μl of Ammonium sulfate (3.5 M)
    291 μl of water (100 v/v)
    C8
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    NA
    250 μl of Glycerol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    321 μl of water (100 v/v)
    C9
    • Sodium formate 3.6 M -
    • Glycerol 10 v/v -
    NA
    514 μl of Sodium formate (7 M)
    100 μl of Glycerol (100 v/v)
    385 μl of water (100 v/v)
    C10
    • Sodium formate 1.4 M -
    • Sodium acetate 0.07 M pH 4.6
    • Glycerol 30 v/v -
    pH 4.6
    200 μl of Sodium formate (7 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    430 μl of water (100 v/v)
    C11
    • HEPES 0.075 M pH 7.5
    • Sodium di-hydrogen phosphate 0.6 M -
    • Potassium di-hydrogen phosphate 0.6 M -
    • Glycerol 25 v/v -
    pH 7.5
    75 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    400 μl of Potassium di-hydrogen phosphate (1.5 M)
    150 μl of Sodium di-hydrogen phosphate (4 M)
    125 μl of water (100 v/v)
    C12
    • tris chloride BCC 0.065 M pH 8.5
    • Polyethylene glycol 8,000 5.2 w/v -
    • Glycerol 35 v/v -
    pH 8.5
    104 μl of Polyethylene glycol 8,000 (50 w/v)
    350 μl of Glycerol (100 v/v)
    65 μl of tris chloride BCC pH 8.5 (1 M)
    481 μl of water (100 v/v)
    D1
    • Sodium acetate 0.07 M pH 4.6
    • Polyethylene glycol 4,000 5.6 w/v -
    • Glycerol 30 v/v -
    pH 4.6
    112 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    518 μl of water (100 v/v)
    D2
    • HEPES 0.09 M pH 7.5
    • tri-Sodium citrate 1.26 M -
    • Glycerol 10 v/v -
    pH 7.5
    90 μl of HEPES pH 7.5 (1 M)
    100 μl of Glycerol (100 v/v)
    787 μl of tri-Sodium citrate (1.6 M)
    22 μl of water (100 v/v)
    D3
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 400 1.7 v/v -
    • Ammonium sulfate 1.7 M -
    • Glycerol 15 v/v -
    pH 7.5
    85 μl of HEPES pH 7.5 (1 M)
    150 μl of Glycerol (100 v/v)
    485 μl of Ammonium sulfate (3.5 M)
    17 μl of Polyethylene glycol 400 (100 v/v)
    262 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.095 M pH 5.6
    • 2-Propanol 19 v/v -
    • Polyethylene glycol 4,000 19 w/v -
    • Glycerol 5 v/v -
    pH 5.6
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Glycerol (100 v/v)
    190 μl of 2-Propanol (100 v/v)
    95 μl of tri-Sodium citrate pH 5.6 (1 M)
    285 μl of water (100 v/v)
    D5
    • HEPES 0.085 M pH 7.5
    • 2-Propanol 8.5 v/v -
    • Polyethylene glycol 4,000 17 w/v -
    • Glycerol 15 v/v -
    pH 7.5
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    85 μl of HEPES pH 7.5 (1 M)
    150 μl of Glycerol (100 v/v)
    85 μl of 2-Propanol (100 v/v)
    340 μl of water (100 v/v)
    D6
    • Potassium di-hydrogen phosphate 0.04 M -
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    NA
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    26 μl of Potassium di-hydrogen phosphate (1.5 M)
    453 μl of water (100 v/v)
    D7
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    NA
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    D8
    • Magnesium formate 0.1 M -
    • Glycerol 50 v/v -
    NA
    100 μl of Magnesium formate (1 M)
    500 μl of Glycerol (100 v/v)
    400 μl of water (100 v/v)
    D9
    • Zinc acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    pH 6.5
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Zinc acetate (1 M)
    272 μl of water (100 v/v)
    D10
    • Calcium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    pH 6.5
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Calcium acetate (1 M)
    272 μl of water (100 v/v)
    D11
    • Ammonium sulfate 1.6 M -
    • Sodium acetate 0.08 M pH 4.6
    • Glycerol 20 v/v -
    pH 4.6
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    262 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.08 M pH 8.5
    • Ammonium di-hydrogen phosphate 1.6 M -
    • Glycerol 20 v/v -
    pH 8.5
    200 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    640 μl of Ammonium di-hydrogen phosphate (2.5 M)
    80 μl of water (100 v/v)
    E1
    • Sodium chloride 1.6 M -
    • Polyethylene glycol 6,000 8 w/v -
    • Glycerol 20 v/v -
    NA
    200 μl of Glycerol (100 v/v)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    320 μl of Sodium chloride (5 M)
    320 μl of water (100 v/v)
    E2
    • Sodium chloride 0.3 M -
    • Magnesium chloride 0.006 M -
    • Cetyltrimethylammonium bromide 10 mM -
    • Glycerol 40 v/v -
    NA
    3 μl of Magnesium chloride (2 M)
    400 μl of Glycerol (100 v/v)
    200 μl of Cetyltrimethylammonium bromide (50 mM)
    60 μl of Sodium chloride (5 M)
    337 μl of water (100 v/v)
    E3
    • Ethylene glycol 21.2 v/v -
    • Glycerol 15 v/v -
    NA
    150 μl of Glycerol (100 v/v)
    212 μl of Ethylene glycol (100 v/v)
    637 μl of water (100 v/v)
    E4
    • 1,4-Dioxane 26.2 v/v -
    • Glycerol 25 v/v -
    NA
    250 μl of Glycerol (100 v/v)
    262 μl of 1,4-Dioxane (100 v/v)
    487 μl of water (100 v/v)
    E5
    • Ammonium sulfate 1.5 M -
    • 2-Propanol 3.75 v/v -
    • Glycerol 25 v/v -
    NA
    250 μl of Glycerol (100 v/v)
    37 μl of 2-Propanol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    283 μl of water (100 v/v)
    E6
    • Imidazole 0.65 M -
    • Glycerol 35 v/v -
    NA
    650 μl of Imidazole pH 7 (1 M)
    350 μl of Glycerol (100 v/v)
    E7
    • Polyethylene glycol 1,000 8 w/v -
    • Polyethylene glycol 8,000 8 w/v -
    • Glycerol 20 v/v -
    NA
    160 μl of Polyethylene glycol 1,000 (50 w/v)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    480 μl of water (100 v/v)
    E8
    • Sodium chloride 1.05 M -
    • Ethanol 7 v/v -
    • Glycerol 30 v/v -
    NA
    300 μl of Glycerol (100 v/v)
    70 μl of Ethanol (100 v/v)
    210 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    E9
    • Sodium acetate 0.08 M pH 4.6
    • Sodium chloride 1.5 M -
    • Glycerol 25 v/v -
    pH 4.6
    250 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Sodium chloride (5 M)
    370 μl of water (100 v/v)
    E10
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Sodium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    560 μl of water (100 v/v)
    E11
    • Cobalt (II) chloride 0.01 M -
    • Sodium acetate 0.08 M pH 4.6
    • 1,6-Hexanediol 0.8 M -
    • Glycerol 20 v/v -
    pH 4.6
    133 μl of 1,6-Hexanediol (6 M)
    10 μl of Cobalt (II) chloride (1 M)
    200 μl of Glycerol (100 v/v)
    80 μl of Sodium acetate pH 4.6 (1 M)
    576 μl of water (100 v/v)
    E12
    • Cadmium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 28.5 v/v -
    • Glycerol 5 v/v -
    pH 4.6
    50 μl of Glycerol (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Cadmium chloride (1 M)
    285 μl of Polyethylene glycol 400 (100 v/v)
    465 μl of water (100 v/v)
    F1
    • Ammonium sulfate 0.18 M -
    • Sodium acetate 0.09 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 27 w/v -
    • Glycerol 10 v/v -
    pH 4.6
    100 μl of Glycerol (100 v/v)
    90 μl of Sodium acetate pH 4.6 (1 M)
    51 μl of Ammonium sulfate (3.5 M)
    540 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    218 μl of water (100 v/v)
    F2
    • Potassium Sodium tartrate 0.15 M -
    • tri-Sodium citrate 0.08 M pH 5.6
    • Ammonium sulfate 1.5 M -
    • Glycerol 25 v/v -
    pH 5.6
    100 μl of Potassium Sodium tartrate (1.5 M)
    250 μl of Glycerol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    141 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.38 M -
    • tri-Sodium citrate 0.08 M pH 5.6
    • Lithium sulfate 0.75 M -
    • Glycerol 25 v/v -
    pH 5.6
    250 μl of Glycerol (100 v/v)
    375 μl of Lithium sulfate (2 M)
    108 μl of Ammonium sulfate (3.5 M)
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    186 μl of water (100 v/v)
    F4
    • Sodium chloride 0.3 M -
    • tri-Sodium citrate 0.06 M pH 5.6
    • Ethylene imine polymer 1.2 v/v -
    • Glycerol 40 v/v -
    pH 5.6
    400 μl of Glycerol (100 v/v)
    60 μl of Sodium chloride (5 M)
    60 μl of tri-Sodium citrate pH 5.6 (1 M)
    24 μl of Ethylene imine polymer (50 v/v)
    456 μl of water (100 v/v)
    F5
    • tri-Sodium citrate 0.08 M pH 5.6
    • 2-Methyl-2-propanol 28 v/v -
    • Glycerol 20 v/v -
    pH 5.6
    200 μl of Glycerol (100 v/v)
    700 μl of 2-Methyl-2-propanol (40 v/v)
    80 μl of tri-Sodium citrate pH 5.6 (1 M)
    20 μl of water (100 v/v)
    F6
    • Iron (III) chloride 0.01 M -
    • tri-Sodium citrate 0.07 M pH 5.6
    • Jeffamine® M-600 7 v/v -
    • Glycerol 30 v/v -
    pH 5.6
    300 μl of Glycerol (100 v/v)
    10 μl of Iron (III) chloride (1 M)
    140 μl of Jeffamine® M-600 pH 7 (50 v/v)
    70 μl of tri-Sodium citrate pH 5.6 (1 M)
    480 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 0.1 M pH 5.6
    • 1,6-Hexanediol 2.38 M -
    • Glycerol 5 v/v -
    pH 5.6
    396 μl of 1,6-Hexanediol (6 M)
    50 μl of Glycerol (100 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    453 μl of water (100 v/v)
    F8
    • MES 0.08 M pH 6.5
    • Magnesium sulfate 1.28 M -
    • Glycerol 20 v/v -
    pH 6.5
    80 μl of MES pH 6.5 (1 M)
    200 μl of Glycerol (100 v/v)
    640 μl of Magnesium sulfate (2 M)
    80 μl of water (100 v/v)
    F9
    • Sodium di-hydrogen phosphate 0.08 M -
    • Potassium di-hydrogen phosphate 0.075 M -
    • MES 0.08 M pH 6.5
    • Sodium chloride 1.5 M -
    • Glycerol 25 v/v -
    pH 6.5
    80 μl of MES pH 6.5 (1 M)
    250 μl of Glycerol (100 v/v)
    50 μl of Potassium di-hydrogen phosphate (1.5 M)
    300 μl of Sodium chloride (5 M)
    20 μl of Sodium di-hydrogen phosphate (4 M)
    300 μl of water (100 v/v)
    F10
    • MES 0.07 M pH 6.5
    • Polyethylene glycol 20,000 7.8 w/v -
    • Glycerol 35 v/v -
    pH 6.5
    70 μl of MES pH 6.5 (1 M)
    350 μl of Glycerol (100 v/v)
    260 μl of Polyethylene glycol 20,000 (30 w/v)
    320 μl of water (100 v/v)
    F11
    • Ammonium sulfate 1.2 M -
    • MES 0.08 M pH 6.5
    • 1,4-Dioxane 7.5 v/v -
    • Glycerol 25 v/v -
    pH 6.5
    80 μl of MES pH 6.5 (1 M)
    250 μl of Glycerol (100 v/v)
    75 μl of 1,4-Dioxane (100 v/v)
    342 μl of Ammonium sulfate (3.5 M)
    252 μl of water (100 v/v)
    F12
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    50 μl of Cesium chloride (1 M)
    250 μl of water (100 v/v)
    G1
    • Cobalt (II) chloride 0.01 M -
    • MES 0.08 M pH 6.5
    • Ammonium sulfate 1.35 M -
    • Glycerol 25 v/v -
    pH 6.5
    10 μl of Cobalt (II) chloride (1 M)
    80 μl of MES pH 6.5 (1 M)
    250 μl of Glycerol (100 v/v)
    385 μl of Ammonium sulfate (3.5 M)
    274 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.18 M -
    • MES 0.09 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 27 w/v -
    • Glycerol 10 v/v -
    pH 6.5
    540 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    90 μl of MES pH 6.5 (1 M)
    100 μl of Glycerol (100 v/v)
    51 μl of Ammonium sulfate (3.5 M)
    218 μl of water (100 v/v)
    G3
    • Zinc sulfate 0.01 M -
    • MES 0.09 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 22.5 v/v -
    • Glycerol 10 v/v -
    pH 6.5
    90 μl of MES pH 6.5 (1 M)
    100 μl of Glycerol (100 v/v)
    225 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    5 μl of Zinc sulfate (2 M)
    580 μl of water (100 v/v)
    G4
    • tri-Sodium citrate 1.6 M -
    NA
    1000 μl of tri-Sodium citrate (1.6 M)
    G5
    • Ammonium sulfate 0.5 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    142 μl of Ammonium sulfate (3.5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    457 μl of water (100 v/v)
    G6
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 6,000 8 w/v -
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • Glycerol 20 v/v -
    pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    200 μl of Glycerol (100 v/v)
    160 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    520 μl of water (100 v/v)
    G7
    • HEPES 0.09 M pH 7.5
    • Jeffamine® M-600 17 v/v -
    • Glycerol 15 v/v -
    pH 7.5
    90 μl of HEPES pH 7.5 (1 M)
    150 μl of Glycerol (100 v/v)
    340 μl of Jeffamine® M-600 pH 7 (50 v/v)
    420 μl of water (100 v/v)
    G8
    • Sodium chloride 0.08 M -
    • HEPES 0.08 M pH 7.5
    • Ammonium sulfate 1.2 M -
    • Glycerol 25 v/v -
    pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    342 μl of Ammonium sulfate (3.5 M)
    16 μl of Sodium chloride (5 M)
    311 μl of water (100 v/v)
    G9
    • HEPES 0.07 M pH 7.5
    • Ammonium formate 1.4 M -
    • Glycerol 30 v/v -
    pH 7.5
    70 μl of HEPES pH 7.5 (1 M)
    300 μl of Glycerol (100 v/v)
    140 μl of Ammonium formate (10 M)
    490 μl of water (100 v/v)
    G10
    • Cadmium sulfate 0.04 M -
    • HEPES 0.08 M pH 7.5
    • Sodium acetate 0.75 M -
    • Glycerol 25 v/v -
    pH 7.5
    40 μl of Cadmium sulfate (1 M)
    250 μl of Sodium acetate (3 M)
    80 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    380 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of water (100 v/v)
    G12
    • HEPES 0.09 M pH 7.5
    • Sodium chloride 3.66 M -
    • Glycerol 15 v/v -
    pH 7.5
    90 μl of HEPES pH 7.5 (1 M)
    150 μl of Glycerol (100 v/v)
    732 μl of Sodium chloride (5 M)
    28 μl of water (100 v/v)
    H1
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 8,000 7.5 w/v -
    • Ethylene glycol 6 v/v -
    • Glycerol 25 v/v -
    pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    150 μl of Polyethylene glycol 8,000 (50 w/v)
    250 μl of Glycerol (100 v/v)
    60 μl of Ethylene glycol (100 v/v)
    460 μl of water (100 v/v)
    H2
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 10,000 15 w/v -
    • Glycerol 25 v/v -
    pH 7.5
    80 μl of HEPES pH 7.5 (1 M)
    250 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    370 μl of water (100 v/v)
    H3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 1,6-Hexanediol 3.4 M -
    pH 8.5
    100 μl of Magnesium chloride (2 M)
    566 μl of 1,6-Hexanediol (6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    233 μl of water (100 v/v)
    H4
    • tris chloride BCC 0.08 M pH 8.5
    • 2-Methyl-2-propanol 18.8 v/v -
    • Glycerol 25 v/v -
    pH 8.5
    250 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    468 μl of 2-Methyl-2-propanol (40 v/v)
    201 μl of water (100 v/v)
    H5
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Lithium sulfate 0.75 M -
    • Glycerol 25 v/v -
    pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    250 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    375 μl of Lithium sulfate (2 M)
    292 μl of water (100 v/v)
    H6
    • Ammonium sulfate 1.28 M -
    • tris chloride BCC 0.09 M pH 8.5
    • Glycerol 25.2 v/v -
    pH 8.5
    252 μl of Glycerol (100 v/v)
    90 μl of tris chloride BCC pH 8.5 (1 M)
    365 μl of Ammonium sulfate (3.5 M)
    292 μl of water (100 v/v)
    H7
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    320 μl of water (100 v/v)
    H8
    • tris chloride BCC 0.08 M pH 8.5
    • Ethanol 15 v/v -
    • Glycerol 25 v/v -
    pH 8.5
    250 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Ethanol (100 v/v)
    520 μl of water (100 v/v)
    H9
    • Nickel (II) chloride 0.01 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    • Glycerol 20 v/v -
    pH 8.5
    2 μl of Nickel (II) chloride (4 M)
    200 μl of Glycerol (100 v/v)
    80 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    397 μl of water (100 v/v)
    H10
    • Sodium chloride 0.09 M -
    • BICINE 0.09 M pH 9
    • Polyethylene glycol monomethyl ether 550 17 v/v -
    • Glycerol 15 v/v -
    pH 9
    150 μl of Glycerol (100 v/v)
    170 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    90 μl of BICINE pH 9 (1 M)
    18 μl of Sodium chloride (5 M)
    572 μl of water (100 v/v)
    H11
    • BICINE 0.1 M pH 9
    • Magnesium chloride 1.9 M -
    • Glycerol 5 v/v -
    pH 9
    50 μl of Glycerol (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    380 μl of Magnesium chloride (5 M)
    470 μl of water (100 v/v)
    H12
    • BICINE 0.07 M pH 9
    • 1,4-Dioxane 1.4 v/v -
    • Polyethylene glycol 20,000 7 w/v -
    • Glycerol 30 v/v -
    pH 9
    300 μl of Glycerol (100 v/v)
    14 μl of 1,4-Dioxane (100 v/v)
    70 μl of BICINE pH 9 (1 M)
    233 μl of Polyethylene glycol 20,000 (30 w/v)
    382 μl of water (100 v/v)