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Supplemental Information

Screen Description

SmartScreen

Microlytic-Anatrace / SmartScreen
Created 2010-04-29.

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SmartScreen

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SmartScreen

    Screen Structure

    SmartScreen

    Well Count 48
    Number of distinct ingredients 42
    Internal diversity (score mean dist) 0.953

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:30:06.862784 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type SmartScreen
    Buffer 54
    Organic (non-volatile) 9
    Organic (volatile) 2
    Polymer 36
    Salt 57

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    SmartScreen Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 400 50 v/v -
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 5.1
    		pH 5.1
    500 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 5.1 (1 M)
    40 μl of Lithium sulfate (5 M)
    0 μl of Sodium acetate pH 4.6 (1 M)
    360 μl of water (100 v/v)
    A2
    • Polyethylene glycol 4,000 30 w/v -
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride pH 7 (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    A3
    • Polyethylene glycol 3,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    500 μl of water (100 v/v)
    A4
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium acetate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    233 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Ammonium citrate 0.2 M pH 7
    		pH 7
    66 μl of tri-Ammonium citrate pH 7 (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    A6
    • Polyethylene glycol 8,000 20 w/v -
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    400 μl of water (100 v/v)
    A7
    • Polyethylene glycol 6,000 20 w/v -
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    		pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of Lithium chloride (5 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    300 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 1.4 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    350 μl of tri-Sodium citrate (4 M)
    550 μl of water (100 v/v)
    B1
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium formate 0.2 M pH 5.9
    		pH 5.9
    200 μl of Magnesium formate pH 5.9 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    B2
    • Polyethylene glycol 400 2 w/v -
    • Ammonium sulfate 2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    40 μl of Polyethylene glycol 400 (50 w/v)
    288 μl of water (100 v/v)
    B3
    • Polyethylene glycol 1,000 20 w/v -
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 4.2
    		pH 4.2
    100 μl of tri-Sodium citrate pH 4.2 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    40 μl of Lithium sulfate (5 M)
    460 μl of water (100 v/v)
    B4
    • Polyethylene glycol 1,500 30 w/v -
    		NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    B5
    • Polyethylene glycol 8,000 20 w/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    0 μl of CHES pH 9 (1 M)
    500 μl of water (100 v/v)
    B6
    • Ammonium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    555 μl of Ammonium sulfate (3.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    344 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium formate 0.2 M pH 6.6
    		pH 6.6
    20 μl of Ammonium formate pH 6.6 (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B8
    • Polyethylene glycol 4,000 30 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of Lithium sulfate (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    260 μl of water (100 v/v)
    C1
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium chloride 0.2 M pH 6.3
    		pH 6.3
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Ammonium chloride pH 6.3 (1 M)
    400 μl of water (100 v/v)
    C2
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    57 μl of Ammonium sulfate (3.5 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    342 μl of water (100 v/v)
    C3
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium formate 0.2 M pH 7.3
    		pH 7.3
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Potassium formate pH 7.3 (1 M)
    400 μl of water (100 v/v)
    C4
    • Polyethylene glycol 8,000 8 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    740 μl of water (100 v/v)
    C5
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    		pH 8.5
    66 μl of Ammonium di-hydrogen phosphate (3 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of water (100 v/v)
    C6
    • Polyethylene glycol 4,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 v/v -
    		pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    C7
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium nitrate 0.2 M pH 6.9
    		pH 6.9
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Potassium nitrate pH 6.9 (3 M)
    533 μl of water (100 v/v)
    C8
    • Polyethylene glycol 8,000 18 w/v -
    • Zinc acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Zinc acetate (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    440 μl of water (100 v/v)
    D1
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    		pH 4
    228 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    671 μl of water (100 v/v)
    D2
    • Calcium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 4.6
    10 μl of Calcium chloride (2 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    590 μl of water (100 v/v)
    D3
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium thiocyanate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate (8 M)
    575 μl of water (100 v/v)
    D4
    • Ammonium di-hydrogen phosphate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    		pH 5.6
    333 μl of Ammonium di-hydrogen phosphate (3 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    566 μl of water (100 v/v)
    D5
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 9
    		pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    D6
    • Polyethylene glycol 400 30 w/v -
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    50 μl of tri-Sodium citrate (4 M)
    600 μl of Polyethylene glycol 400 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of water (100 v/v)
    D7
    • Polyethylene glycol 8,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 8 v/v -
    		pH 7.5
    160 μl of Ethylene glycol (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    540 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 28 w/v -
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium chloride (2 M)
    560 μl of Polyethylene glycol 400 (50 w/v)
    240 μl of water (100 v/v)
    E1
    • Polyethylene glycol 8,000 5 w/v -
    • Sodium cacodylate 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 0.4 v/v -
    		pH 7
    4 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    0 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Sodium cacodylate pH 7 (1 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    796 μl of water (100 v/v)
    E2
    • Lithium sulfate 1.5 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Lithium sulfate (5 M)
    600 μl of water (100 v/v)
    E3
    • Polyethylene glycol 1,000 5 w/v -
    • tri-Sodium citrate 0.1 M pH 5.2
    • Ethanol 0.4 v/v -
    		pH 5.2
    100 μl of tri-Sodium citrate pH 5.2 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    4 μl of Ethanol (100 v/v)
    796 μl of water (100 v/v)
    E4
    • Polyethylene glycol 4,000 25 w/v -
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    342 μl of water (100 v/v)
    E5
    • Polyethylene glycol 4,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    740 μl of water (100 v/v)
    E6
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    		pH 6.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Magnesium acetate (2 M)
    500 μl of water (100 v/v)
    E7
    • Polyethylene glycol 8,000 10 w/v -
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    600 μl of water (100 v/v)
    E8
    • Polyethylene glycol 8,000 30 w/v -
    • Sodium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium acetate (2 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of water (100 v/v)
    F1
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    F2
    • Sodium formate 4 M -
    		NA
    1000 μl of Sodium formate (4 M)
    F3
    • Polyethylene glycol 200 50 v/v -
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.6
    		pH 6.6
    100 μl of Magnesium chloride (2 M)
    100 μl of Sodium cacodylate pH 6.6 (1 M)
    0 μl of Sodium cacodylate pH 6.5 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    300 μl of water (100 v/v)
    F4
    • Sodium formate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    285 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    F5
    • tri-Sodium citrate 1.6 M pH 6.5
    		pH 6.5
    1000 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    F6
    • Sodium di-hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 0.8 M -
    		pH 7.5
    200 μl of Potassium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    266 μl of Sodium di-hydrogen phosphate (3 M)
    433 μl of water (100 v/v)
    F7
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Potassium citrate 0.2 M pH 8.3
    		pH 8.3
    40 μl of tri-Potassium citrate pH 8.3 (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    F8
    • Polyethylene glycol 8,000 20 w/v -
    • Potassium di-hydrogen phosphate 0.05 M -
    		NA
    50 μl of Potassium di-hydrogen phosphate (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    550 μl of water (100 v/v)