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Supplemental Information

Screen Description

SmartScreen

Microlytic-Anatrace / SmartScreen
Created 2010-04-29.

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SmartScreen

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Screen Structure

SmartScreen

Well Count 48
Number of distinct ingredients 42
Internal diversity (score mean dist) 0.953

Screen Composition

2025-08-16T06:25:41.808915 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type SmartScreen
Buffer 54
Organic (non-volatile) 9
Organic (volatile) 2
Polymer 36
Salt 57

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
SmartScreen Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 50 v/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 5.1
pH 5.1
40.0 μl of Lithium sulfate (5 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
-0.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of Sodium acetate pH 5.1 (1 M)
360.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 4,000 30 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
66.7 μl of Ammonium acetate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Ammonium citrate 0.2 M pH 7
pH 7
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 7 (3 M)
533.3 μl of water (100 v/v)
A6
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
300.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 1.4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
350.0 μl of tri-Sodium citrate (4 M)
550.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M pH 5.9
pH 5.9
200.0 μl of Magnesium formate pH 5.9 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 400 2 w/v -
  • Ammonium sulfate 2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 400 (50 w/v)
288.6 μl of water (100 v/v)
B3
  • Polyethylene glycol 1,000 20 w/v -
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 4.2
pH 4.2
40.0 μl of Lithium sulfate (5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
-0.0 μl of CHES pH 9 (1 M)
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
B6
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
555.6 μl of Ammonium sulfate (3.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
344.4 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M pH 6.6
pH 6.6
20.0 μl of Ammonium formate pH 6.6 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40.0 μl of Lithium sulfate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
260.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M pH 6.3
pH 6.3
200.0 μl of Ammonium chloride pH 6.3 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.9 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M pH 7.3
pH 7.3
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium formate pH 7.3 (1 M)
400.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 8,000 8 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
740.0 μl of water (100 v/v)
C5
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 20 w/v -
  • HEPES 0.1 M pH 7.5
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M pH 6.9
pH 6.9
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate pH 6.9 (3 M)
533.3 μl of water (100 v/v)
C8
  • Polyethylene glycol 8,000 18 w/v -
  • Zinc acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
100.0 μl of Zinc acetate (2 M)
440.0 μl of water (100 v/v)
D1
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
671.4 μl of water (100 v/v)
D2
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
D4
  • Ammonium di-hydrogen phosphate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
566.7 μl of water (100 v/v)
D5
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 400 30 w/v -
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 400 (50 w/v)
50.0 μl of tri-Sodium citrate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 8 v/v -
pH 7.5
160.0 μl of Ethylene glycol (50 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
540.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 400 28 w/v -
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 400 (50 w/v)
240.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 8,000 5 w/v -
  • Sodium cacodylate 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 0.4 v/v -
pH 7
4.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
-0.0 μl of Sodium cacodylate pH 6.5 (1 M)
100.0 μl of Sodium cacodylate pH 7 (1 M)
796.0 μl of water (100 v/v)
E2
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Lithium sulfate (5 M)
600.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 1,000 5 w/v -
  • tri-Sodium citrate 0.1 M pH 5.2
  • Ethanol 0.4 v/v -
pH 5.2
4.0 μl of Ethanol (100 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.2 (1 M)
796.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
342.9 μl of water (100 v/v)
E5
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
E6
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Magnesium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 8,000 10 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
600.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
F2
  • Sodium formate 4 M -
NA
1000.0 μl of Sodium formate (4 M)
F3
  • Polyethylene glycol 200 50 v/v -
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.6
pH 6.6
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
-0.0 μl of Sodium cacodylate pH 6.5 (1 M)
100.0 μl of Sodium cacodylate pH 6.6 (1 M)
300.0 μl of water (100 v/v)
F4
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
F5
  • tri-Sodium citrate 1.6 M pH 6.5
pH 6.5
1000.0 μl of tri-Sodium citrate pH 6.5 (1.6 M)
F6
  • Sodium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
  • Potassium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Potassium di-hydrogen phosphate (4 M)
266.7 μl of Sodium di-hydrogen phosphate (3 M)
433.3 μl of water (100 v/v)
F7
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M pH 8.3
pH 8.3
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of tri-Potassium citrate pH 8.3 (5 M)
560.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 8,000 20 w/v -
  • Potassium di-hydrogen phosphate 0.05 M -
NA
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate (1 M)
550.0 μl of water (100 v/v)