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Supplemental Information

Screen Description

PurePegs

Microlytic-Anatrace / PurePegs

Created 2010-04-29.

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PurePegs

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PurePegs

Screen Structure

PurePegs

Well Count	48
Number of distinct ingredients	57
Internal diversity (score mean dist)	0.769

Screen Composition

More information about screen composition chart.

ingredient type	PurePegs
Buffer	60
Organic (non-volatile)	1
Polymer	49
Salt	62

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

PurePegs Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	PurePEGs 0.3-8 kD 22.5 w/v - Ammonium chloride 0.3 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 60 μl of Ammonium chloride (5 M) 390 μl of water (100 v/v)
A2	PurePEGs 0.3-8 kD 22.5 w/v - Ammonium formate 0.3 M pH 6.6 Potassium nitrate 0.1 M - 🔍	pH 6.6	30 μl of Ammonium formate pH 6.6 (10 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 33 μl of Potassium nitrate (3 M) 486 μl of water (100 v/v)
A3	PurePEGs 0.3-8 kD 22.5 w/v - Ammonium nitrate 0.3 M - tri-Sodium citrate 0.1 M pH 5.9 🔍	pH 5.9	30 μl of Ammonium nitrate (10 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of tri-Sodium citrate pH 5.9 (1 M) 420 μl of water (100 v/v)
A4	PurePEGs 0.3-8 kD 22.5 w/v - Ammonium di-hydrogen phosphate 0.3 M - MES 0.1 M pH 5.7 🔍	pH 5.7	450 μl of PurePEGs 0.3-8 kD (50 w/v) 120 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of MES pH 5.7 (1 M) 330 μl of water (100 v/v)
A5	PurePEGs 0.3-8 kD 22.5 w/v - BIS-TRIS 0.1 M pH 6.8 Ammonium sulfate 0.3 M - 🔍	pH 6.8	85 μl of Ammonium sulfate (3.5 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of BIS-TRIS pH 6.8 (1 M) 364 μl of water (100 v/v)
A6	PurePEGs 0.3-8 kD 22.5 w/v - Sodium acetate 0.1 M pH 5.8 di-Ammonium tartrate 0.3 M - 🔍	pH 5.8	450 μl of PurePEGs 0.3-8 kD (50 w/v) 150 μl of di-Ammonium tartrate (2 M) 15 μl of Sodium acetate pH 5 (1 M) 84 μl of Sodium acetate pH 6.2 (1 M) 300 μl of water (100 v/v)
A7	PurePEGs 0.3-8 kD 22.5 w/v - Cadmium chloride 0.3 M - Sodium acetate 0.1 M pH 3.7 🔍	pH 3.7	450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of Cadmium chloride (1 M) 100 μl of Sodium acetate pH 3.7 (1 M) 150 μl of water (100 v/v)
A8	PurePEGs 0.3-8 kD 22.5 w/v - Imidazole 0.1 M pH 4.6 Cadmium sulfate 0.3 M - 🔍	pH 4.6	450 μl of PurePEGs 0.3-8 kD (50 w/v) 97 μl of Imidazole pH 4.5 (1 M) 3 μl of Imidazole pH 5.5 (1 M) 300 μl of Cadmium sulfate (1 M) 150 μl of water (100 v/v)
B1	PurePEGs 0.3-8 kD 22.5 w/v - HEPES 0.1 M pH 7.6 Cesium chloride 0.3 M - 🔍	pH 7.6	450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of Cesium chloride (1 M) 100 μl of HEPES pH 7.6 (1 M) 150 μl of water (100 v/v)
B2	PurePEGs 0.3-8 kD 22.5 w/v - BICINE 0.1 M pH 6.4 Calcium acetate 0.3 M - 🔍	pH 6.4	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of BICINE pH 6.4 (1 M) 300 μl of Calcium acetate (1 M) 150 μl of water (100 v/v)
B3	PurePEGs 0.3-8 kD 22.5 w/v - BIS-TRIS propane 0.1 M pH 8.5 Calcium chloride 0.3 M - 🔍	pH 8.5	100 μl of BIS-TRIS propane pH 8.5 (1 M) 150 μl of Calcium chloride (2 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of water (100 v/v)
B4	PurePEGs 0.3-8 kD 22.5 w/v - di-Ammonium tartrate 0.1 M pH 3.2 Cobalt (II) chloride 0.3 M - 🔍	pH 3.2	450 μl of PurePEGs 0.3-8 kD (50 w/v) 33 μl of di-Ammonium tartrate pH 3.2 (3 M) 300 μl of Cobalt (II) chloride (1 M) 216 μl of water (100 v/v)
B5	PurePEGs 0.3-8 kD 22.5 w/v - di-Ammonium hydrogen citrate 0.3 M - Magnesium formate 0.1 M - 🔍	NA	450 μl of PurePEGs 0.3-8 kD (50 w/v) 120 μl of di-Ammonium hydrogen citrate pH 4.9 (2.5 M) 100 μl of Magnesium formate (1 M) 330 μl of water (100 v/v)
B6	PurePEGs 0.3-8 kD 22.5 w/v - di-Sodium DL-malate 0.3 M pH 2.9 Sodium cacodylate 0.1 M - 🔍	pH 2.9	100 μl of di-Sodium DL-malate pH 2.9 (3 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Sodium cacodylate (1 M) 350 μl of water (100 v/v)
B7	PurePEGs 0.3-8 kD 22.5 w/v - Iron (III) chloride 0.3 M - Potassium nitrate 0.1 M - 🔍	NA	33 μl of Potassium nitrate pH 1.1 (3 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of Iron (III) chloride (1 M) 216 μl of water (100 v/v)
B8	PurePEGs 0.3-8 kD 22.5 w/v - Lithium chloride 0.3 M - Potassium formate 0.1 M pH 6.3 🔍	pH 6.3	100 μl of Potassium formate pH 6.3 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 30 μl of Lithium chloride (10 M) 420 μl of water (100 v/v)
C1	PurePEGs 0.3-8 kD 22.5 w/v - CHES 0.1 M pH 9.5 Lithium sulfate 0.3 M - 🔍	pH 9.5	450 μl of PurePEGs 0.3-8 kD (50 w/v) 150 μl of Lithium sulfate (2 M) 100 μl of CHES pH 9.5 (1 M) 300 μl of water (100 v/v)
C2	PurePEGs 0.3-8 kD 22.5 w/v - Magnesium acetate 0.3 M - tri-Sodium citrate 0.1 M pH 4.7 🔍	pH 4.7	300 μl of Magnesium acetate (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of tri-Sodium citrate pH 4.7 (1 M) 150 μl of water (100 v/v)
C3	PurePEGs 0.3-8 kD 22.5 w/v - Sodium cacodylate 0.1 M pH 5.8 Magnesium chloride 0.3 M - 🔍	pH 5.8	100 μl of Sodium cacodylate pH 5.8 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 150 μl of Magnesium chloride (2 M) 300 μl of water (100 v/v)
C4	PurePEGs 0.3-8 kD 22.5 w/v - HEPES 0.1 M pH 7.4 Magnesium nitrate 0.3 M - 🔍	pH 7.4	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Magnesium nitrate (3 M) 100 μl of HEPES pH 7.4 (1 M) 350 μl of water (100 v/v)
C5	PurePEGs 0.3-8 kD 22.5 w/v - Potassium formate 0.1 M pH 6.1 Magnesium sulfate 0.1 M - 🔍	pH 6.1	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Potassium formate pH 6.1 (1 M) 50 μl of Magnesium sulfate (2 M) 400 μl of water (100 v/v)
C6	PurePEGs 0.3-8 kD 22.5 w/v - MES 0.1 M pH 5.7 Nickel (II) chloride 0.3 M - 🔍	pH 5.7	75 μl of Nickel (II) chloride (4 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of MES pH 5.7 (1 M) 375 μl of water (100 v/v)
C7	PurePEGs 0.3-8 kD 22.5 w/v - BIS-TRIS 0.1 M pH 6.6 Potassium bromide 0.3 M - 🔍	pH 6.6	450 μl of PurePEGs 0.3-8 kD (50 w/v) 75 μl of Potassium bromide (4 M) 100 μl of BIS-TRIS pH 6.6 (1 M) 375 μl of water (100 v/v)
C8	PurePEGs 0.3-8 kD 22.5 w/v - tris chloride BCC 0.1 M pH 6.1 Potassium di-hydrogen phosphate 0.3 M - 🔍	pH 6.1	450 μl of PurePEGs 0.3-8 kD (50 w/v) 24 μl of tris chloride BCC pH 6 (3 M) 200 μl of Potassium di-hydrogen phosphate (1.5 M) 8 μl of tris chloride BCC pH 6.3 (3 M) 316 μl of water (100 v/v)
D1	PurePEGs 0.3-8 kD 22.5 w/v - BICINE 0.1 M pH 8.8 Potassium Sodium tartrate 0.3 M - 🔍	pH 8.8	200 μl of Potassium Sodium tartrate (1.5 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of BICINE pH 8.8 (1 M) 250 μl of water (100 v/v)
D2	PurePEGs 0.3-8 kD 22.5 w/v - Imidazole 0.1 M pH 7.3 di-Potassium tartrate 0.3 M - 🔍	pH 7.3	300 μl of di-Potassium tartrate (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Imidazole pH 7.3 (1 M) 150 μl of water (100 v/v)
D3	PurePEGs 0.3-8 kD 22.5 w/v - di-Ammonium tartrate 0.1 M pH 5.8 🔍	pH 5.8	450 μl of PurePEGs 0.3-8 kD (50 w/v) 25 μl of di-Ammonium tartrate pH 5.5 (2 M) 24 μl of di-Ammonium tartrate pH 8.5 (2 M) 500 μl of water (100 v/v)
D4	PurePEGs 0.3-8 kD 22.5 w/v - Sodium bromide 0.3 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 60 μl of Sodium bromide (5 M) 390 μl of water (100 v/v)
D5	PurePEGs 0.3-8 kD 22.5 w/v - tris chloride BCC 0.1 M pH 8.1 Sodium chloride 0.3 M - 🔍	pH 8.1	60 μl of Sodium chloride (5 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of tris chloride BCC pH 8.1 (1 M) 390 μl of water (100 v/v)
D6	PurePEGs 0.3-8 kD 22.5 w/v - CHES 0.1 M pH 9.5 Sodium fluoride 0.3 M - 🔍	pH 9.5	450 μl of PurePEGs 0.3-8 kD (50 w/v) 375 μl of Sodium fluoride (0.8 M) 100 μl of CHES pH 9.5 (1 M) 75 μl of water (100 v/v)
D7	PurePEGs 0.3-8 kD 22.5 w/v - tri-Sodium citrate 0.1 M pH 4.5 Sodium formate 0.3 M - 🔍	pH 4.5	100 μl of tri-Sodium citrate pH 4.5 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 42 μl of Sodium formate (7 M) 407 μl of water (100 v/v)
D8	PurePEGs 0.3-8 kD 22.5 w/v - Sodium acetate 0.1 M pH 4.7 Sodium iodide 0.3 M - 🔍	pH 4.7	100 μl of Sodium acetate pH 4.7 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of Sodium iodide (1 M) 150 μl of water (100 v/v)
E1	PurePEGs 0.3-8 kD 22.5 w/v - tri-Sodium citrate 0.1 M pH 6.8 di-Sodium malonate 0.3 M - 🔍	pH 6.8	450 μl of PurePEGs 0.3-8 kD (50 w/v) 0 μl of tri-Sodium citrate pH 5.8 (1 M) 99 μl of tri-Sodium citrate pH 7 (1 M) 88 μl of di-Sodium malonate pH 4 (3.4 M) 361 μl of water (100 v/v)
E2	PurePEGs 0.3-8 kD 22.5 w/v - MES 0.1 M pH 6.1 Sodium nitrate 0.3 M - 🔍	pH 6.1	42 μl of Sodium nitrate (7 M) 100 μl of MES pH 6.1 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 407 μl of water (100 v/v)
E3	PurePEGs 0.3-8 kD 22.5 w/v - BIS-TRIS 0.1 M pH 5.8 Sodium di-hydrogen phosphate 0.3 M - 🔍	pH 5.8	100 μl of BIS-TRIS pH 5.8 (1 M) 75 μl of Sodium di-hydrogen phosphate (4 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 375 μl of water (100 v/v)
E4	PurePEGs 0.3-8 kD 22.5 w/v - BIS-TRIS propane 0.1 M pH 9.3 Sodium sulfate 0.3 M - 🔍	pH 9.3	300 μl of Sodium sulfate (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of BIS-TRIS propane pH 9.3 (1 M) 150 μl of water (100 v/v)
E5	PurePEGs 0.3-8 kD 22.5 w/v - HEPES 0.1 M pH 7.8 Sodium thiocyanate 0.3 M - 🔍	pH 7.8	100 μl of HEPES pH 7.8 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 37 μl of Sodium thiocyanate (8 M) 412 μl of water (100 v/v)
E6	PurePEGs 0.3-8 kD 22.5 w/v - di-Sodium succinate 0.3 M - Imidazole 0.1 M - 🔍	NA	450 μl of PurePEGs 0.3-8 kD (50 w/v) 300 μl of di-Sodium succinate pH 3.4 (1 M) 100 μl of Imidazole (1 M) 150 μl of water (100 v/v)
E7	PurePEGs 0.3-8 kD 22.5 w/v - BICINE 0.1 M pH 8.6 Trimethylamine N-oxide 0.3 M - 🔍	pH 8.6	300 μl of Trimethylamine N-oxide (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of BICINE pH 8.6 (1 M) 150 μl of water (100 v/v)
E8	PurePEGs 0.3-8 kD 22.5 w/v - tri-Potassium citrate 0.3 M - BIS-TRIS propane 0.1 M pH 9.8 🔍	pH 9.8	450 μl of PurePEGs 0.3-8 kD (50 w/v) 120 μl of tri-Potassium citrate (2.5 M) 100 μl of BIS-TRIS propane pH 9.8 (1 M) 330 μl of water (100 v/v)
F1	PurePEGs 0.3-8 kD 22.5 w/v - HEPES 0.1 M pH 7.5 Jeffamine® M-600 10 v/v - 🔍	pH 7.5	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Jeffamine® M-600 pH 7 (50 v/v) 250 μl of water (100 v/v)
F2	PurePEGs 0.3-8 kD 22.5 w/v - Magnesium formate 0.1 M pH 5.7 Zinc chloride 0.3 M - 🔍	pH 5.7	450 μl of PurePEGs 0.3-8 kD (50 w/v) 150 μl of Zinc chloride (2 M) 100 μl of Magnesium formate pH 5.7 (1 M) 300 μl of water (100 v/v)
F3	PurePEGs 0.3-8 kD 22.5 w/v - Zinc sulfate 0.3 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 150 μl of Zinc sulfate (2 M) 300 μl of water (100 v/v)
F4	PurePEGs 0.3-8 kD 22.5 w/v - CHES 0.1 M pH 9.9 🔍	pH 9.9	450 μl of PurePEGs 0.3-8 kD (50 w/v) 52 μl of CHES pH 10 (1 M) 47 μl of CHES pH 9.5 (1 M) 450 μl of water (100 v/v)
F5	PurePEGs 0.3-8 kD 22.5 w/v - Imidazole 0.1 M pH 6.6 🔍	pH 6.6	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Imidazole pH 6.6 (1 M) 450 μl of water (100 v/v)
F6	PurePEGs 0.3-8 kD 22.5 w/v - Magnesium formate 0.1 M pH 5.9 🔍	pH 5.9	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of Magnesium formate pH 5.9 (1 M) 450 μl of water (100 v/v)
F7	PurePEGs 0.3-8 kD 22.5 w/v - Sodium acetate 0.1 M pH 4.7 🔍	pH 4.7	100 μl of Sodium acetate pH 4.7 (1 M) 450 μl of PurePEGs 0.3-8 kD (50 w/v) 450 μl of water (100 v/v)
F8	PurePEGs 0.3-8 kD 22.5 w/v - tris chloride BCC 0.1 M pH 8.1 🔍	pH 8.1	450 μl of PurePEGs 0.3-8 kD (50 w/v) 100 μl of tris chloride BCC pH 8.1 (1 M) 450 μl of water (100 v/v)