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Supplemental Information

Screen Description

Salt Screen HT

Hampton Research / Salt Screen HT
Created 2010-04-29.

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Salt Screen HT

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Salt Screen HT

    Screen Structure

    Salt Screen HT

    Well Count 96
    Number of distinct ingredients 10
    Internal diversity (score mean dist) 0.815

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:44:14.655283 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Salt Screen HT
    Buffer 120
    Salt 136

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Salt Screen HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 0.8 M -
    		pH 5
    228 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    671 μl of water (100 v/v)
    A3
    • MES 0.1 M pH 6
    • Ammonium sulfate 0.8 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 0.8 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.8 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 0.8 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 1.6 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 1.6 M -
    		pH 5
    457 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    442 μl of water (100 v/v)
    A9
    • MES 0.1 M pH 6
    • Ammonium sulfate 1.6 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    A10
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 1.6 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 1.6 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    A12
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 1.6 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 2.4 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 2.4 M -
    		pH 5
    685 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    214 μl of water (100 v/v)
    B3
    • MES 0.1 M pH 6
    • Ammonium sulfate 2.4 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 2.4 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 2.4 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    B6
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    B7
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 3 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 3 M -
    		pH 5
    857 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    42 μl of water (100 v/v)
    B9
    • MES 0.1 M pH 6
    • Ammonium sulfate 3 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    B10
    • HEPES 0.1 M pH 7
    • Ammonium sulfate 3 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    B11
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 3 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    B12
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 3 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    857 μl of Ammonium sulfate (3.5 M)
    42 μl of water (100 v/v)
    C1
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 4 (3.4 M)
    705 μl of water (100 v/v)
    C2
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 4 (3.4 M)
    558 μl of water (100 v/v)
    C3
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 4 (3.4 M)
    441 μl of water (100 v/v)
    C4
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 4 (3.4 M)
    294 μl of water (100 v/v)
    C5
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 4 (3.4 M)
    147 μl of water (100 v/v)
    C6
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 4 (3.4 M)
    C7
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 5 (3.4 M)
    705 μl of water (100 v/v)
    C8
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 5 (3.4 M)
    558 μl of water (100 v/v)
    C9
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 5 (3.4 M)
    441 μl of water (100 v/v)
    C10
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 5 (3.4 M)
    294 μl of water (100 v/v)
    C11
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 5 (3.4 M)
    147 μl of water (100 v/v)
    C12
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 5 (3.4 M)
    D1
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 6 (3.4 M)
    705 μl of water (100 v/v)
    D2
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 6 (3.4 M)
    558 μl of water (100 v/v)
    D3
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 6 (3.4 M)
    441 μl of water (100 v/v)
    D4
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 6 (3.4 M)
    294 μl of water (100 v/v)
    D5
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 6 (3.4 M)
    147 μl of water (100 v/v)
    D6
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 6 (3.4 M)
    D7
    • di-Sodium malonate 1 M -
    		NA
    294 μl of di-Sodium malonate pH 7 (3.4 M)
    705 μl of water (100 v/v)
    D8
    • di-Sodium malonate 1.5 M -
    		NA
    441 μl of di-Sodium malonate pH 7 (3.4 M)
    558 μl of water (100 v/v)
    D9
    • di-Sodium malonate 1.9 M -
    		NA
    558 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    D10
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    D11
    • di-Sodium malonate 2.9 M -
    		NA
    852 μl of di-Sodium malonate pH 7 (3.4 M)
    147 μl of water (100 v/v)
    D12
    • di-Sodium malonate 3.4 M -
    		NA
    1000 μl of di-Sodium malonate pH 7 (3.4 M)
    E1
    • Sodium di-hydrogen phosphate 0.784 M -
    • di-Potassium hydrogen phosphate 0.016 M -
    		NA
    4 μl of di-Potassium hydrogen phosphate (4 M)
    196 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    E2
    • Sodium di-hydrogen phosphate 0.98 M -
    • di-Potassium hydrogen phosphate 0.02 M -
    		NA
    5 μl of di-Potassium hydrogen phosphate (4 M)
    245 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E3
    • Sodium di-hydrogen phosphate 1.37 M -
    • di-Potassium hydrogen phosphate 0.028 M -
    		NA
    7 μl of di-Potassium hydrogen phosphate (4 M)
    343 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    E4
    • Sodium di-hydrogen phosphate 1.76 M -
    • di-Potassium hydrogen phosphate 0.036 M -
    		NA
    9 μl of di-Potassium hydrogen phosphate (4 M)
    441 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    E5
    • Sodium di-hydrogen phosphate 0.72 M -
    • di-Potassium hydrogen phosphate 0.08 M -
    		NA
    20 μl of di-Potassium hydrogen phosphate (4 M)
    180 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    E6
    • Sodium di-hydrogen phosphate 0.9 M -
    • di-Potassium hydrogen phosphate 0.1 M -
    		NA
    25 μl of di-Potassium hydrogen phosphate (4 M)
    225 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E7
    • Sodium di-hydrogen phosphate 1.26 M -
    • di-Potassium hydrogen phosphate 0.14 M -
    		NA
    35 μl of di-Potassium hydrogen phosphate (4 M)
    315 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    E8
    • Sodium di-hydrogen phosphate 1.62 M -
    • di-Potassium hydrogen phosphate 0.18 M -
    		NA
    45 μl of di-Potassium hydrogen phosphate (4 M)
    405 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    E9
    • Sodium di-hydrogen phosphate 0.52 M -
    • di-Potassium hydrogen phosphate 0.28 M -
    		NA
    70 μl of di-Potassium hydrogen phosphate (4 M)
    130 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    E10
    • Sodium di-hydrogen phosphate 0.65 M -
    • di-Potassium hydrogen phosphate 0.35 M -
    		NA
    87 μl of di-Potassium hydrogen phosphate (4 M)
    162 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E11
    • Sodium di-hydrogen phosphate 0.91 M -
    • di-Potassium hydrogen phosphate 0.49 M -
    		NA
    122 μl of di-Potassium hydrogen phosphate (4 M)
    227 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    E12
    • Sodium di-hydrogen phosphate 1.17 M -
    • di-Potassium hydrogen phosphate 0.63 M -
    		NA
    157 μl of di-Potassium hydrogen phosphate (4 M)
    292 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    F1
    • Sodium di-hydrogen phosphate 0.28 M -
    • di-Potassium hydrogen phosphate 0.52 M -
    		NA
    130 μl of di-Potassium hydrogen phosphate (4 M)
    70 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    F2
    • Sodium di-hydrogen phosphate 0.35 M -
    • di-Potassium hydrogen phosphate 0.65 M -
    		NA
    162 μl of di-Potassium hydrogen phosphate (4 M)
    87 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    F3
    • Sodium di-hydrogen phosphate 0.49 M -
    • di-Potassium hydrogen phosphate 0.91 M -
    		NA
    227 μl of di-Potassium hydrogen phosphate (4 M)
    122 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    F4
    • Sodium di-hydrogen phosphate 0.63 M -
    • di-Potassium hydrogen phosphate 1.17 M -
    		NA
    292 μl of di-Potassium hydrogen phosphate (4 M)
    157 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    F5
    • Sodium di-hydrogen phosphate 0.12 M -
    • di-Potassium hydrogen phosphate 0.68 M -
    		NA
    170 μl of di-Potassium hydrogen phosphate (4 M)
    30 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    F6
    • Sodium di-hydrogen phosphate 0.15 M -
    • di-Potassium hydrogen phosphate 0.85 M -
    		NA
    212 μl of di-Potassium hydrogen phosphate (4 M)
    37 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    F7
    • Sodium di-hydrogen phosphate 0.21 M -
    • di-Potassium hydrogen phosphate 1.19 M -
    		NA
    297 μl of di-Potassium hydrogen phosphate (4 M)
    52 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    F8
    • Sodium di-hydrogen phosphate 0.27 M -
    • di-Potassium hydrogen phosphate 1.53 M -
    		NA
    382 μl of di-Potassium hydrogen phosphate (4 M)
    67 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    F9
    • Sodium di-hydrogen phosphate 0.032 M -
    • di-Potassium hydrogen phosphate 0.768 M -
    		NA
    192 μl of di-Potassium hydrogen phosphate (4 M)
    8 μl of Sodium di-hydrogen phosphate (4 M)
    800 μl of water (100 v/v)
    F10
    • Sodium di-hydrogen phosphate 0.04 M -
    • di-Potassium hydrogen phosphate 0.96 M -
    		NA
    240 μl of di-Potassium hydrogen phosphate (4 M)
    10 μl of Sodium di-hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    F11
    • Sodium di-hydrogen phosphate 0.056 M -
    • di-Potassium hydrogen phosphate 1.34 M -
    		NA
    336 μl of di-Potassium hydrogen phosphate (4 M)
    14 μl of Sodium di-hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    F12
    • Sodium di-hydrogen phosphate 0.072 M -
    • di-Potassium hydrogen phosphate 1.73 M -
    		NA
    432 μl of di-Potassium hydrogen phosphate (4 M)
    18 μl of Sodium di-hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    G1
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 1 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    G2
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 1 M -
    		pH 5
    200 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    700 μl of water (100 v/v)
    G3
    • MES 0.1 M pH 6
    • Sodium chloride 1 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    G4
    • HEPES 0.1 M pH 7
    • Sodium chloride 1 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 1 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    G6
    • BICINE 0.1 M pH 9
    • Sodium chloride 1 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    200 μl of Sodium chloride (5 M)
    700 μl of water (100 v/v)
    G7
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 2 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    G8
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 2 M -
    		pH 5
    400 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    G9
    • MES 0.1 M pH 6
    • Sodium chloride 2 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    G10
    • HEPES 0.1 M pH 7
    • Sodium chloride 2 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    G11
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    G12
    • BICINE 0.1 M pH 9
    • Sodium chloride 2 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    400 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    H1
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 3 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H2
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 3 M -
    		pH 5
    600 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    300 μl of water (100 v/v)
    H3
    • MES 0.1 M pH 6
    • Sodium chloride 3 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H4
    • HEPES 0.1 M pH 7
    • Sodium chloride 3 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H5
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 3 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H6
    • BICINE 0.1 M pH 9
    • Sodium chloride 3 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    H7
    • tri-Sodium citrate 0.1 M pH 4
    • Sodium chloride 4 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    H8
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 4 M -
    		pH 5
    800 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of water (100 v/v)
    H9
    • MES 0.1 M pH 6
    • Sodium chloride 4 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    H10
    • HEPES 0.1 M pH 7
    • Sodium chloride 4 M -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 4 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    H12
    • BICINE 0.1 M pH 9
    • Sodium chloride 4 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)