C6 - Screen Contents - Natrix Screen 2

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Supplemental Information

Screen Description

Natrix Screen 2

Hampton Research / Natrix Screen 2

Created 2010-04-29.

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Natrix Screen 2

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Natrix Screen 2

Screen Structure

Natrix Screen 2

Well Count	48
Number of distinct ingredients	30
Internal diversity (score mean dist)	0.768

Screen Composition

More information about screen composition chart.

ingredient type	Natrix Screen 2
Buffer	64
Complex ingredient	3
Organic (non-volatile)	31
Organic (volatile)	6
Polymer	5
Salt	122

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Natrix Screen 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Lithium chloride 40 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - Cobalt (III) Hexamine chloride 20 mM - 🔍	pH 6.5	200 μl of Cobalt (III) Hexamine chloride (100 mM) 40 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80 μl of Lithium chloride (500 mM) 40 μl of Magnesium chloride (500 mM) 340 μl of water (100 v/v)
A2	Sodium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 35 v/v - Cobalt (III) Hexamine chloride 20 mM - 🔍	pH 6.5	200 μl of Cobalt (III) Hexamine chloride (100 mM) 40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Magnesium chloride (500 mM) 210 μl of water (100 v/v)
A3	Sodium chloride 12 mM - Potassium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 45 v/v - Cobalt (III) Hexamine chloride 20 mM - 🔍	pH 6.5	200 μl of Cobalt (III) Hexamine chloride (100 mM) 40 μl of Sodium cacodylate pH 6.5 (1 M) 24 μl of Sodium chloride (500 mM) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 126 μl of water (100 v/v)
A4	Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Cobalt (III) Hexamine chloride 20 mM - 🔍	pH 6.5	200 μl of Cobalt (III) Hexamine chloride (100 mM) 40 μl of Sodium cacodylate pH 6.5 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Magnesium chloride (500 mM) 320 μl of water (100 v/v)
A5	Calcium chloride 2 mM - Sodium cacodylate 0.05 M pH 6.5 Ammonium sulfate 1.8 M - Spermine 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 5 μl of Spermine (100 mM) 20 μl of Calcium chloride (100 mM) 514 μl of Ammonium sulfate (3.5 M) 410 μl of water (100 v/v)
A6	Sodium cacodylate 0.05 M pH 6.5 Tacsimate 35 v/v - Spermine 1 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 10 μl of Spermine (100 mM) 350 μl of Tacsimate pH 7 (100 v/v) 590 μl of water (100 v/v)
A7	Sodium chloride 0.1 M - Sodium cacodylate 0.05 M pH 6.5 Polyethylene glycol 4,000 10 w/v - Spermine 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 5 μl of Spermine (100 mM) 20 μl of Sodium chloride (5 M) 725 μl of water (100 v/v)
A8	Potassium chloride 50 mM - Sodium cacodylate 0.05 M pH 6.5 Polyethylene glycol 8,000 10 w/v - Spermine 0.5 mM - L-Argininamide dihydrochloride 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 5 μl of L-Argininamide dihydrochloride (100 mM) 5 μl of Spermine (100 mM) 100 μl of Potassium chloride (500 mM) 200 μl of Polyethylene glycol 8,000 (50 w/v) 640 μl of water (100 v/v)
B1	Potassium chloride 0.1 M - Sodium cacodylate 0.05 M pH 6.5 Polyethylene glycol 1,000 16 w/v - Spermine 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 320 μl of Polyethylene glycol 1,000 (50 w/v) 5 μl of Spermine (100 mM) 25 μl of Potassium chloride (4 M) 600 μl of water (100 v/v)
B2	Magnesium chloride 5 mM - Calcium chloride 2 mM - Sodium cacodylate 0.05 M pH 6.5 2-Propanol 15 v/v - Spermine 1 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 10 μl of Spermine (100 mM) 20 μl of Calcium chloride (100 mM) 150 μl of 2-Propanol (100 v/v) 10 μl of Magnesium chloride (500 mM) 760 μl of water (100 v/v)
B3	Sodium chloride 75 mM - Calcium chloride 2 mM - Sodium cacodylate 0.05 M pH 6.5 1,6-Hexanediol 30 w/v - Spermine 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 150 μl of Sodium chloride (500 mM) 5 μl of Spermine (100 mM) 300 μl of 1,6-Hexanediol (100 w/v) 20 μl of Calcium chloride (100 mM) 475 μl of water (100 v/v)
B4	Magnesium sulfate 20 mM - Cobalt (II) chloride 2 mM - Sodium cacodylate 0.05 M pH 6.5 2-methyl-2,4-pentanediol BCC 25 v/v - Spermine 0.5 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 100 μl of Magnesium sulfate (200 mM) 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 5 μl of Spermine (100 mM) 10 μl of Cobalt (II) chloride (200 mM) 585 μl of water (100 v/v)
B5	Sodium cacodylate 0.05 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 650 μl of water (100 v/v)
B6	Sodium chloride 80 mM - Potassium chloride 12 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 24 μl of Potassium chloride (500 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 376 μl of water (100 v/v)
B7	Sodium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 35 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 350 μl of water (100 v/v)
B8	Strontium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 35 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of Strontium chloride (200 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 150 μl of water (100 v/v)
C1	Potassium chloride 80 mM - Barium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 200 μl of Barium chloride (100 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 140 μl of water (100 v/v)
C2	Potassium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 45 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 250 μl of water (100 v/v)
C3	Sodium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 45 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 60 μl of Spermine Tetrahydrochloride (200 mM) 290 μl of water (100 v/v)
C4	Sodium chloride 80 mM - Barium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 45 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Barium chloride (100 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 90 μl of water (100 v/v)
C5	Sodium chloride 12 mM - Potassium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 50 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 24 μl of Sodium chloride (500 mM) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 216 μl of water (100 v/v)
C6	Potassium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 55 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 550 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 190 μl of water (100 v/v)
C7	Magnesium chloride 18 mM - Sodium cacodylate 0.05 M pH 6.5 2-Propanol 10 v/v - Spermine 3 mM - 🔍	pH 6.5	50 μl of Sodium cacodylate pH 6.5 (1 M) 30 μl of Spermine (100 mM) 100 μl of 2-Propanol (100 v/v) 36 μl of Magnesium chloride (500 mM) 784 μl of water (100 v/v)
C8	Magnesium chloride 20 mM - MOPS 0.05 M pH 7 Ammonium sulfate 2 M - Spermine 0.5 mM - 🔍	pH 7	50 μl of MOPS pH 7 (1 M) 5 μl of Spermine (100 mM) 40 μl of Magnesium chloride (500 mM) 571 μl of Ammonium sulfate (3.5 M) 333 μl of water (100 v/v)
D1	HEPES 0.05 M pH 7.5 Tacsimate 40 v/v - Spermine 2 mM - Cobalt (III) Hexamine chloride 2 mM - 🔍	pH 7.5	20 μl of Cobalt (III) Hexamine chloride (100 mM) 20 μl of Spermine (100 mM) 50 μl of HEPES pH 7.5 (1 M) 400 μl of Tacsimate pH 7 (100 v/v) 510 μl of water (100 v/v)
D2	Magnesium chloride 20 mM - MOPS 0.05 M pH 7 Tacsimate 55 v/v - Cobalt (III) Hexamine chloride 5 mM - 🔍	pH 7	50 μl of Cobalt (III) Hexamine chloride (100 mM) 50 μl of MOPS pH 7 (1 M) 550 μl of Tacsimate pH 7 (100 v/v) 40 μl of Magnesium chloride (500 mM) 310 μl of water (100 v/v)
D3	Magnesium chloride 20 mM - Sodium cacodylate 0.05 M pH 6.5 2-Propanol 15 v/v - Cobalt (III) Hexamine chloride 1 mM - Spermine 1 mM - 🔍	pH 6.5	10 μl of Cobalt (III) Hexamine chloride (100 mM) 50 μl of Sodium cacodylate pH 6.5 (1 M) 10 μl of Spermine (100 mM) 150 μl of 2-Propanol (100 v/v) 40 μl of Magnesium chloride (500 mM) 740 μl of water (100 v/v)
D4	Magnesium chloride 5 mM - MOPS 0.05 M pH 7 1,4-Dioxane 25 v/v - Spermine 1 mM - 🔍	pH 7	250 μl of 1,4-Dioxane (100 v/v) 50 μl of MOPS pH 7 (1 M) 10 μl of Spermine (100 mM) 10 μl of Magnesium chloride (500 mM) 680 μl of water (100 v/v)
D5	Magnesium chloride 10 mM - Barium chloride 2 mM - MOPS 0.05 M pH 7 1,4-Dioxane 30 v/v - 🔍	pH 7	300 μl of 1,4-Dioxane (100 v/v) 50 μl of MOPS pH 7 (1 M) 20 μl of Barium chloride (100 mM) 20 μl of Magnesium chloride (500 mM) 610 μl of water (100 v/v)
D6	Magnesium chloride 1 mM - Calcium chloride 2 mM - MOPS 0.05 M pH 7 2-methyl-2,4-pentanediol BCC 15 v/v - 🔍	pH 7	150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of MOPS pH 7 (1 M) 20 μl of Calcium chloride (100 mM) 2 μl of Magnesium chloride (500 mM) 778 μl of water (100 v/v)
D7	Strontium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 20 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of Strontium chloride (200 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 260 μl of water (100 v/v)
D8	Sodium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 60 μl of Spermine Tetrahydrochloride (200 mM) 440 μl of water (100 v/v)
E1	Lithium chloride 40 mM - Strontium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80 μl of Lithium chloride (500 mM) 400 μl of Strontium chloride (200 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 120 μl of water (100 v/v)
E2	Lithium chloride 0.04 M - Strontium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 4 μl of Lithium chloride (10 M) 400 μl of Strontium chloride (200 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 156 μl of water (100 v/v)
E3	Sodium chloride 80 mM - Potassium chloride 12 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 35 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 24 μl of Potassium chloride (500 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 326 μl of water (100 v/v)
E4	Sodium chloride 12 mM - Potassium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 24 μl of Sodium chloride (500 mM) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 316 μl of water (100 v/v)
E5	Sodium chloride 80 mM - Barium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Barium chloride (100 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 140 μl of water (100 v/v)
E6	Sodium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Sodium chloride (500 mM) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 300 μl of water (100 v/v)
E7	Potassium chloride 80 mM - Barium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 200 μl of Barium chloride (100 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 140 μl of water (100 v/v)
E8	Potassium chloride 80 mM - Magnesium chloride 20 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 50 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 40 μl of Magnesium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 200 μl of water (100 v/v)
F1	Potassium chloride 80 mM - Sodium cacodylate 0.04 M pH 6.5 2-methyl-2,4-pentanediol BCC 60 v/v - Spermine Tetrahydrochloride 12 mM - 🔍	pH 6.5	40 μl of Sodium cacodylate pH 6.5 (1 M) 600 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Potassium chloride (500 mM) 60 μl of Spermine Tetrahydrochloride (200 mM) 140 μl of water (100 v/v)
F2	Magnesium chloride 20 mM - Cobalt (II) chloride 2 mM - HEPES 0.05 M pH 7.5 Ammonium sulfate 2 M - Spermine 1 mM - 🔍	pH 7.5	10 μl of Spermine (100 mM) 50 μl of HEPES pH 7.5 (1 M) 10 μl of Cobalt (II) chloride (200 mM) 40 μl of Magnesium chloride (500 mM) 571 μl of Ammonium sulfate (3.5 M) 318 μl of water (100 v/v)
F3	Magnesium chloride 20 mM - PIPES 0.05 M pH 7.5 Polyethylene glycol 8,000 4 w/v - Spermine 1 mM - 🔍	pH 7.5	10 μl of Spermine (100 mM) 50 μl of PIPES pH 7.5 (1 M) 40 μl of Magnesium chloride (500 mM) 80 μl of Polyethylene glycol 8,000 (50 w/v) 820 μl of water (100 v/v)
F4	Magnesium chloride 15 mM - Barium chloride 2 mM - PIPES 0.05 M pH 7.5 2-Propanol 7 v/v - Spermine 0.5 mM - 🔍	pH 7.5	5 μl of Spermine (100 mM) 50 μl of PIPES pH 7.5 (1 M) 70 μl of 2-Propanol (100 v/v) 20 μl of Barium chloride (100 mM) 30 μl of Magnesium chloride (500 mM) 825 μl of water (100 v/v)
F5	Magnesium chloride 20 mM - PIPES 0.05 M pH 7.5 1,6-Hexanediol 10 w/v - Spermine 1 mM - 🔍	pH 7.5	10 μl of Spermine (100 mM) 50 μl of PIPES pH 7.5 (1 M) 333 μl of 1,6-Hexanediol (30 w/v) 40 μl of Magnesium chloride (500 mM) 566 μl of water (100 v/v)
F6	Magnesium chloride 10 mM - HEPES 0.05 M pH 7.5 2-methyl-2,4-pentanediol BCC 15 v/v - Spermine 1.5 mM - 🔍	pH 7.5	150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 15 μl of Spermine (100 mM) 50 μl of HEPES pH 7.5 (1 M) 20 μl of Magnesium chloride (500 mM) 765 μl of water (100 v/v)
F7	Calcium chloride 0.2 mM - HEPES 0.05 M pH 7.5 Polyethylene glycol 400 28 v/v - Spermine 2 mM - 🔍	pH 7.5	280 μl of Polyethylene glycol 400 (100 v/v) 20 μl of Spermine (100 mM) 50 μl of HEPES pH 7.5 (1 M) 2 μl of Calcium chloride (100 mM) 648 μl of water (100 v/v)
F8	Copper (II) chloride 2 mM - tris chloride BCC 0.05 M pH 8.5 Lithium sulfate 1.8 M - Spermine 0.5 mM - 🔍	pH 8.5	5 μl of Spermine (100 mM) 20 μl of Copper (II) chloride (100 mM) 50 μl of tris chloride BCC pH 8.5 (1 M) 900 μl of Lithium sulfate (2 M) 25 μl of water (100 v/v)