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Supplemental Information

Screen Description

Wizard I&II

Rigaku / Wizard I&II
Created 2010-05-28.

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Wizard I&II

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Wizard I&II

    Screen Structure

    Wizard I&II

    Well Count 96
    Number of distinct ingredients 32
    Internal diversity (score mean dist) 0.933

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:22:19.372657 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Wizard I&II
    Buffer 114
    Organic (non-volatile) 12
    Organic (volatile) 16
    Polymer 41
    Salt 130

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Wizard I&II Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 8,000 20 w/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    A2
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    40 μl of Sodium chloride (5 M)
    100 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    760 μl of water (100 v/v)
    A3
    • Ethanol 15 v/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    150 μl of Ethanol (100 v/v)
    750 μl of water (100 v/v)
    A4
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Imidazole 0.1 M pH 8
    • Magnesium chloride 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Magnesium chloride (2 M)
    450 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 30 v/v -
    • CAPS 0.1 M pH 10
    		pH 10
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of CAPS pH 10 (1 M)
    600 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    25 μl of tri-Sodium citrate pH 5.5 (4 M)
    575 μl of water (100 v/v)
    A7
    • Polyethylene glycol 8,000 10 w/v -
    • MES 0.1 M pH 6
    • Zinc acetate 0.2 M -
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    200 μl of Zinc acetate (1 M)
    117 μl of MES pH 5.2 (0.5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of water (100 v/v)
    A8
    • Ammonium sulfate 2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    571 μl of Ammonium sulfate (3.5 M)
    25 μl of tri-Sodium citrate pH 5.5 (4 M)
    403 μl of water (100 v/v)
    A9
    • di-Ammonium hydrogen phosphate 1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    681 μl of water (100 v/v)
    A10
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    A11
    • 1,4-Butanediol 20 v/v -
    • MES 0.1 M pH 6
    • Lithium sulfate 0.2 M -
    		pH 6
    200 μl of 1,4-Butanediol (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    80 μl of Lithium sulfate (2.5 M)
    520 μl of water (100 v/v)
    A12
    • Polyethylene glycol 1,000 20 w/v -
    • Imidazole 0.1 M pH 8
    • Calcium acetate 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    B1
    • Ammonium sulfate 1.26 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    360 μl of Ammonium sulfate (3.5 M)
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    540 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 1 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,000 10 w/v -
    • Imidazole 0.1 M pH 8
    • Lithium sulfate 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    620 μl of water (100 v/v)
    B4
    • Sodium chloride 2.5 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    		pH 6.2
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    500 μl of Sodium chloride (5 M)
    475 μl of water (100 v/v)
    B5
    • Polyethylene glycol 8,000 30 w/v -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    		pH 4.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    286 μl of water (100 v/v)
    B6
    • Potassium Sodium tartrate 1 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    666 μl of Potassium Sodium tartrate (1.5 M)
    193 μl of water (100 v/v)
    B7
    • Polyethylene glycol 1,000 20 w/v -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    500 μl of water (100 v/v)
    B8
    • Sodium di-hydrogen phosphate 0.4 M -
    • di-Potassium hydrogen phosphate 1.6 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    80 μl of Sodium di-hydrogen phosphate (5 M)
    533 μl of di-Potassium hydrogen phosphate (3 M)
    246 μl of water (100 v/v)
    B9
    • Polyethylene glycol 8,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    B10
    • 2-Propanol 10 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of water (100 v/v)
    B11
    • Ethanol 15 v/v -
    • Imidazole 0.1 M pH 8
    • Magnesium chloride 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    100 μl of Magnesium chloride (2 M)
    150 μl of Ethanol (100 v/v)
    650 μl of water (100 v/v)
    B12
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • tris chloride BCC 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    510 μl of water (100 v/v)
    C1
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    C2
    • Polyethylene glycol 3,000 10 w/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    700 μl of water (100 v/v)
    C3
    • Sodium di-hydrogen phosphate 1.2 M -
    • di-Potassium hydrogen phosphate 0.8 M -
    • CAPS 0.1 M pH 10
    • Lithium sulfate 0.2 M -
    		pH 10
    80 μl of Lithium sulfate (2.5 M)
    240 μl of Sodium di-hydrogen phosphate (5 M)
    266 μl of di-Potassium hydrogen phosphate (3 M)
    100 μl of CAPS pH 10 (1 M)
    313 μl of water (100 v/v)
    C4
    • Polyethylene glycol 3,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    C5
    • Polyethylene glycol 8,000 10 w/v -
    • CHES 0.1 M pH 9.5
    • Sodium chloride 0.2 M -
    		pH 9.5
    40 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CHES pH 9.5 (1 M)
    660 μl of water (100 v/v)
    C6
    • Ammonium sulfate 1.26 M -
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 0.2 M -
    		pH 4.5
    360 μl of Ammonium sulfate (3.5 M)
    40 μl of Sodium chloride (5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    566 μl of water (100 v/v)
    C7
    • Polyethylene glycol 8,000 20 w/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Sodium chloride 0.2 M -
    		pH 4.2
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    460 μl of water (100 v/v)
    C8
    • Polyethylene glycol 3,000 10 w/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    		pH 6.2
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    775 μl of water (100 v/v)
    C9
    • Ammonium sulfate 2 M -
    • CAPS 0.1 M pH 10
    • Lithium sulfate 0.2 M -
    		pH 10
    571 μl of Ammonium sulfate (3.5 M)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of CAPS pH 10 (1 M)
    248 μl of water (100 v/v)
    C10
    • di-Ammonium hydrogen phosphate 1 M -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    614 μl of water (100 v/v)
    C11
    • 1,4-Butanediol 20 v/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    200 μl of 1,4-Butanediol (100 v/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    766 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 1 M -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    D1
    • Sodium chloride 2.5 M -
    • Imidazole 0.1 M pH 8
    		pH 8
    500 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    400 μl of water (100 v/v)
    D2
    • Potassium Sodium tartrate 1 M -
    • CHES 0.1 M pH 9.5
    • Lithium sulfate 0.2 M -
    		pH 9.5
    666 μl of Potassium Sodium tartrate (1.5 M)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of CHES pH 9.5 (1 M)
    153 μl of water (100 v/v)
    D3
    • Polyethylene glycol 1,000 20 w/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Lithium sulfate 0.2 M -
    		pH 4.2
    80 μl of Lithium sulfate (2.5 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    420 μl of water (100 v/v)
    D4
    • 2-Propanol 10 v/v -
    • MES 0.1 M pH 6
    • Calcium acetate 0.2 M -
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    100 μl of 2-Propanol (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    200 μl of Calcium acetate (1 M)
    500 μl of water (100 v/v)
    D5
    • Polyethylene glycol 3,000 30 w/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    300 μl of water (100 v/v)
    D6
    • Ethanol 15 v/v -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    150 μl of Ethanol (100 v/v)
    750 μl of water (100 v/v)
    D7
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    		pH 6.2
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    625 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 30 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Calcium acetate 0.2 M -
    		pH 4.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    200 μl of Calcium acetate (1 M)
    466 μl of water (100 v/v)
    D9
    • Polyethylene glycol 3,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    566 μl of water (100 v/v)
    D10
    • Polyethylene glycol 8,000 10 w/v -
    • Imidazole 0.1 M pH 8
    • Calcium acetate 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    500 μl of water (100 v/v)
    D11
    • Ammonium sulfate 1.26 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    360 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    460 μl of water (100 v/v)
    D12
    • Polyethylene glycol 1,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.5
    • Zinc acetate 0.2 M -
    		pH 4.5
    200 μl of Zinc acetate (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    366 μl of water (100 v/v)
    E1
    • Polyethylene glycol 3,000 10 w/v -
    • Sodium acetate 0.1 M pH 4.5
    • Zinc acetate 0.2 M -
    		pH 4.5
    200 μl of Zinc acetate (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    566 μl of water (100 v/v)
    E2
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • MES 0.1 M pH 6
    • Lithium sulfate 0.2 M -
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    80 μl of Lithium sulfate (2.5 M)
    370 μl of water (100 v/v)
    E3
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    400 μl of water (100 v/v)
    E4
    • Ammonium sulfate 2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Sodium chloride 0.2 M -
    		pH 6.5
    571 μl of Ammonium sulfate (3.5 M)
    40 μl of Sodium chloride (5 M)
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    288 μl of water (100 v/v)
    E5
    • 1,4-Butanediol 20 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    200 μl of 1,4-Butanediol (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    660 μl of water (100 v/v)
    E6
    • 2-Propanol 10 v/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Lithium sulfate 0.2 M -
    		pH 4.2
    100 μl of 2-Propanol (100 v/v)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    720 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,000 30 w/v -
    • tris chloride BCC 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    260 μl of water (100 v/v)
    E8
    • Polyethylene glycol 8,000 10 w/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Sodium chloride 0.2 M -
    		pH 6.2
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    40 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    735 μl of water (100 v/v)
    E9
    • Ammonium sulfate 2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    		pH 4.2
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    328 μl of water (100 v/v)
    E10
    • di-Ammonium hydrogen phosphate 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    614 μl of water (100 v/v)
    E11
    • 2-Propanol 10 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Zinc acetate 0.2 M -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Zinc acetate (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    100 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    E12
    • Polyethylene glycol 400 30 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Lithium sulfate 0.2 M -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    F1
    • Ethanol 15 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Lithium sulfate 0.2 M -
    		pH 5.5
    80 μl of Lithium sulfate (2.5 M)
    150 μl of Ethanol (100 v/v)
    25 μl of tri-Sodium citrate pH 5.5 (4 M)
    745 μl of water (100 v/v)
    F2
    • Polyethylene glycol 1,000 20 w/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Sodium chloride 0.2 M -
    		pH 6.2
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    535 μl of water (100 v/v)
    F3
    • Ammonium sulfate 1.26 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    360 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    540 μl of water (100 v/v)
    F4
    • tri-Sodium citrate 1 M -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    F5
    • Sodium chloride 2.5 M -
    • tris chloride BCC 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of Sodium chloride (5 M)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    F6
    • Polyethylene glycol 3,000 20 w/v -
    • tris chloride BCC 0.1 M pH 7
    • Calcium acetate 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    F7
    • Sodium di-hydrogen phosphate 1.6 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • di-Potassium hydrogen phosphate 0.4 M -
    		pH 4.2
    320 μl of Sodium di-hydrogen phosphate (5 M)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    133 μl of di-Potassium hydrogen phosphate (3 M)
    446 μl of water (100 v/v)
    F8
    • Ethanol 15 v/v -
    • MES 0.1 M pH 6
    • Zinc acetate 0.2 M -
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    200 μl of Zinc acetate (1 M)
    117 μl of MES pH 5.2 (0.5 M)
    150 μl of Ethanol (100 v/v)
    450 μl of water (100 v/v)
    F9
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    616 μl of water (100 v/v)
    F10
    • 2-Propanol 10 v/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    100 μl of 2-Propanol (100 v/v)
    800 μl of water (100 v/v)
    F11
    • Ethanol 15 v/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    		pH 7.5
    100 μl of Magnesium chloride (2 M)
    150 μl of Ethanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    650 μl of water (100 v/v)
    F12
    • Polyethylene glycol 8,000 30 w/v -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    260 μl of water (100 v/v)
    G1
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    40 μl of Sodium chloride (5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    510 μl of water (100 v/v)
    G2
    • Polyethylene glycol 400 30 v/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    G3
    • Polyethylene glycol 3,000 10 w/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    600 μl of water (100 v/v)
    G4
    • Polyethylene glycol 8,000 20 w/v -
    • MES 0.1 M pH 6
    • Calcium acetate 0.2 M -
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    200 μl of water (100 v/v)
    G5
    • Ammonium sulfate 1.26 M -
    • CHES 0.1 M pH 9.5
    • Sodium chloride 0.2 M -
    		pH 9.5
    360 μl of Ammonium sulfate (3.5 M)
    40 μl of Sodium chloride (5 M)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    G6
    • 1,4-Butanediol 20 v/v -
    • Imidazole 0.1 M pH 8
    • Zinc acetate 0.2 M -
    		pH 8
    200 μl of 1,4-Butanediol (100 v/v)
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Zinc acetate (1 M)
    500 μl of water (100 v/v)
    G7
    • tri-Sodium citrate 1 M -
    • tris chloride BCC 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    625 μl of tri-Sodium citrate (1.6 M)
    235 μl of water (100 v/v)
    G8
    • Polyethylene glycol 1,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    500 μl of water (100 v/v)
    G9
    • di-Ammonium hydrogen phosphate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.2 M -
    		pH 5.5
    40 μl of Sodium chloride (5 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    25 μl of tri-Sodium citrate pH 5.5 (4 M)
    649 μl of water (100 v/v)
    G10
    • Polyethylene glycol 8,000 10 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    700 μl of water (100 v/v)
    G11
    • Sodium di-hydrogen phosphate 0.8 M -
    • Potassium di-hydrogen phosphate 1.2 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    800 μl of Potassium di-hydrogen phosphate (1.5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    160 μl of Sodium di-hydrogen phosphate (5 M)
    6 μl of water (100 v/v)
    G12
    • Polyethylene glycol 3,000 10 w/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Sodium chloride 0.2 M -
    		pH 4.2
    40 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    660 μl of water (100 v/v)
    H1
    • Potassium Sodium tartrate 1 M -
    • tris chloride BCC 0.1 M pH 7
    • Lithium sulfate 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    666 μl of Potassium Sodium tartrate (1.5 M)
    80 μl of Lithium sulfate (2.5 M)
    153 μl of water (100 v/v)
    H2
    • Sodium chloride 2.5 M -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    		pH 4.5
    500 μl of Sodium chloride (5 M)
    80 μl of Lithium sulfate (2.5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    386 μl of water (100 v/v)
    H3
    • Polyethylene glycol 8,000 20 w/v -
    • CAPS 0.1 M pH 10
    • Sodium chloride 0.2 M -
    		pH 10
    40 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CAPS pH 10 (1 M)
    460 μl of water (100 v/v)
    H4
    • Polyethylene glycol 3,000 20 w/v -
    • Imidazole 0.1 M pH 8
    • Zinc acetate 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Zinc acetate (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    300 μl of water (100 v/v)
    H5
    • Ammonium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 7
    • Lithium sulfate 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    80 μl of Lithium sulfate (2.5 M)
    248 μl of water (100 v/v)
    H6
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    40 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of water (100 v/v)
    H7
    • Polyethylene glycol 8,000 10 w/v -
    • tris chloride BCC 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    600 μl of water (100 v/v)
    H8
    • Polyethylene glycol 1,000 20 w/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    400 μl of water (100 v/v)
    H9
    • Ammonium sulfate 1.26 M -
    • MES 0.1 M pH 6
    		pH 6
    360 μl of Ammonium sulfate (3.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    440 μl of water (100 v/v)
    H10
    • di-Ammonium hydrogen phosphate 1 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 0.2 M -
    		pH 8
    40 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    574 μl of water (100 v/v)
    H11
    • Sodium chloride 2.5 M -
    • Imidazole 0.1 M pH 8
    • Zinc acetate 0.2 M -
    		pH 8
    500 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Zinc acetate (1 M)
    200 μl of water (100 v/v)
    H12
    • Potassium Sodium tartrate 1 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    666 μl of Potassium Sodium tartrate (1.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    133 μl of water (100 v/v)